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This paper reviews the basic research means for oilfield development and also the researches and tests of enhanced oil recovery (EOR) methods for mature oilfields and continental shale oil development, analyzes the problems of EOR methods, and proposes the relevant research prospects. The basic research means for oilfield development include in-situ acquisition of formation rock/fluid samples and non-destructive testing. The EOR methods for conventional and shale oil development are classified as improved water flooding (e.g. nano-water flooding), chemical flooding (e.g. low-concentration middle-phase micro-emulsion flooding), gas flooding (e.g. micro/nano bubble flooding), thermal recovery (e.g. air injection thermal-aided miscible flooding), and multi-cluster uniform fracturing/water-free fracturing, which are discussed in this paper for their mechanisms, approaches, and key technique researches and field tests. These methods have been studied with remarkable progress, and some achieved ideal results in field tests. Nonetheless, some problems still exist, such as inadequate research on mechanisms, imperfect matching technologies, and incomplete industrial chains. It is proposed to further strengthen the basic researches and expand the field tests, thereby driving the formation, promotion and application of new technologies.

Abstract Facial palsy (FP) profoundly influences interpersonal communication and emotional expression, necessitating precise diagnostic and monitoring tools for optimal care. However, current electromyography (EMG) systems are limited by their bulky nature, complex setups, and dependence on skilled technicians. Here we report an innovative biosensing approach that utilizes a PEDOT:PSS-modified flexible microneedle electrode array (P-FMNEA) to overcome the limitations of existing EMG devices. Supple system-level mechanics ensure excellent conformality to the facial curvilinear regions, enabling the detection of targeted muscular ensemble movements for facial paralysis assessment. Moreover, our apparatus adeptly captures each electrical impulse in response to real-time direct nerve stimulation during neurosurgical procedures. The wireless conveyance of EMG signals to medical facilities via a server augments access to patient follow-up evaluation data, fostering prompt treatment suggestions and enabling the access of multiple facial EMG datasets during typical 6-month follow-ups. Furthermore, the device’s soft mechanics alleviate issues of spatial intricacy, diminish pain, and minimize soft tissue hematomas associated with traditional needle electrode positioning. This groundbreaking biosensing strategy has the potential to transform FP management by providing an efficient, user-friendly, and less invasive alternative to the prevailing EMG devices. This pioneering technology enables more informed decision-making in FP-management and therapeutic intervention.

Water has the disadvantages of low viscosity, poor lubrication, and easy leakage, which leads to many problems in water hydraulic flow control valves, such as low working pressure and large flow fluctuations. To address these issues, this paper proposes a novel digital flow control valve. The valve uses a linear stepper motor as the driving device. Compared to proportional electromagnets, the thrust and stroke of the linear stepper motor are larger, making the valve more suitable for high-pressure working conditions. Simultaneously, the valve innovatively incorporates a set of pilot valve spool strings at the front end of the pilot valve damping hole. Through controlling the two pilot valves to regulate the pressure difference before and after the damping hole, the flow passing through the pilot valve is maintained stable, thereby making the pressure of the upper chamber of the master valve spool more stable. In comparison to a single pilot valve structure, this design ensures a more stable main valve core position and reduces flow fluctuation. A mathematical and simulation model of the valve has been established, confirming the performance advantages of the new structure. The impact of structural parameters (such as valve core diameter, spring stiffness, and diameter of damping hole) on the stability of flow regulation has been investigated. A genetic algorithm has been employed to optimize the key parameters that influence valve flow stability, resulting in the identification of optimal parameters. The simulation results indicate that the optimized parameters lead to a reduction of approximately 45% in the maximum overshoot oscillation amplitude of the valve flow regulation. A prototype of the new flow control valve was developed, and a test system was established for conducting tests. The test results also confirmed the performance advantages of the valve and the accuracy of the optimal design.

Abstract Identifying new targets for overcoming radioresistance is crucial for improving the efficacy of lung cancer radiotherapy, given that tumor cell resistance is a leading cause of treatment failure. Recent research has spotlighted the significance of Musashi2 (MSI2) in cancer biology. In this study, we first demonstrated that MSI2 plays a key function in regulating the radiosensitivity of lung cancer. The expression of MSI2 is negatively correlated with overall survival in cancer patients, and the knockdown of MSI2 inhibits tumorigenesis and increases radiosensitivity of lung cancer cells. Cellular radiosensitivity, which is closely linked to DNA damage, is influenced by MSI2 interaction with ataxia telangiectasia mutated and Rad3‐related kinase (ATR) and checkpoint kinase 1 (CHK1) post‐irradiation; moreover, knockdown of MSI2 inhibits the ATR‐mediated DNA damage response pathway. RNA‐binding motif protein 17 (RBM17), which is implicated in DNA damage repair, exhibits increased interaction with MSI2 post‐irradiation. We found that knockdown of RBM17 disrupted the interaction between MSI2 and ATR post‐irradiation and increased the radiosensitivity of lung cancer cells. Furthermore, we revealed the potential mechanism of MSI2 recruitment into the nucleus with the assistance of RBM17 to activate ATR to promote radioresistance. This study provides novel insights into the potential application of MSI2 as a new target in lung cancer radiotherapy.

To address the issue of insufficient transverse connectivity in prestressed concrete box girder (PCB) bridges, this study investigates two transverse strengthening methods—installing diaphragms and utilizing concrete-filled steel tube trusses (CFSTTs). A finite element model was developed for a typical 30 m PCB bridge and was validated by on-site load test results for reliability. Based on the deflection and load distribution of PCB bridges before and after reinforcement, as well as the maximum stress and strain of the diaphragms and the CFSTTs, comparative analyses were conducted on diaphragms of different thicknesses and materials, as well as on CFSTTs of various strength grades. The results show that the addition of a transverse partition and CFSTTs can effectively improve the load distribution of the PCB bridge and reduce the maximum deflection of the girder, especially when using the CFSTT reinforcement method. The unique structural design improves the reinforcement effect of the material in the post-elastic stage. When using CFSTTs, increasing the steel tube wall strength significantly reduces the maximum deflection of the main girder. For example, using steel tubes with yield strengths of 235 MPa and 420 MPa filled with concrete of 50 MPa compressive strength reduced the maximum deflections by 15.32% and 24.55%, respectively, and improved the load distribution coefficients by up to 7.31% and 11.57%. Additionally, steel diaphragms demonstrated better reinforcement effects compared with concrete diaphragms. The load transverse distribution coefficients for the CFSTT-reinforced PCB bridge were calculated using the hinge plate (beam) and the rigid plate (beam) methods, showing minimal differences between the two approaches. The findings of this study provide valuable insights into the design of diaphragm and CFSTT reinforcement in PCB bridges, aiding in the selection of optimal reinforcement strategies.

Aiming at the four issues of underground storage state, exploitation mechanism, crude oil flow and efficient recovery, the key theoretical and technical issues and countermeasures for effective development of Gulong shale oil are put forward. Through key exploration and research on fluid occurrence, fluid phase change, exploitation mechanism, oil start-up mechanism, flow regime/pattern, exploitation mode and enhanced oil recovery (EOR) of shale reservoirs with different storage spaces, multi-scale occurrence states of shale oil and phase behavior of fluid in nano confined space were provided, the multi-phase, multi-scale flow mode and production mechanism with hydraulic fracture-shale bedding fracture-matrix infiltration as the core was clarified, and a multi-scale flow mathematical model and recoverable reserves evaluation method were preliminarily established. The feasibility of development mode with early energy replenishment and recovery factor of 30% was discussed. Based on these, the researches of key theories and technologies for effective development of Gulong shale oil are proposed to focus on: (1) in-situ sampling and non-destructive testing of core and fluid; (2) high-temperature, high-pressure, nano-scale laboratory simulation experiment; (3) fusion of multi-scale multi-flow regime numerical simulation technology and large-scale application software; (4) waterless (CO2) fracturing technique and the fracturing technique for increasing the vertical fracture height; (5) early energy replenishment to enhance oil recovery; (6) lifecycle technical and economic evaluation. Moreover, a series of exploitation tests should be performed on site as soon as possible to verify the theoretical understanding, optimize the exploitation mode, form supporting technologies, and provide a generalizable development model, thereby supporting and guiding the effective development and production of Gulong shale oil.

Abstract Background Ganciclovir (GCV) is widely used in solid organ and haematopoietic stem cell transplant patients for prophylaxis and treatment of cytomegalovirus. It has long been considered a mutagen and carcinogen. However, the contribution of GCV to cancer incidence and other factors that influence its mutagenicity remains unknown. Methods This retrospective cohort study analysed genomics data for 121,771 patients who had undergone targeted sequencing compiled by the Genomics Evidence Neoplasia Information Exchange (GENIE) or Foundation Medicine (FM). A statistical approach was developed to identify patients with GCV-associated mutational signature (GCVsig) from targeted sequenced data of tumour samples. Cell line exposure models were further used to quantify mutation burden and DNA damage caused by GCV and other antiviral and immunosuppressive drugs. Results Mutational profiles from 22 of 121,771 patient samples in the GENIE and FM cohorts showed evidence of GCVsig. A diverse range of cancers was represented. All patients with detailed clinical history available had previously undergone solid organ transplantation and received GCV and mycophenolate treatment. RAS hotspot mutations associated with GCVsig were present in 9 of the 22 samples, with all samples harbouring multiple GCV-associated protein-altering mutations in cancer driver genes. In vitro testing in cell lines showed that elevated DNA damage response and GCVsig are uniquely associated with GCV but not acyclovir, a structurally similar antiviral. Combination treatment of GCV with the immunosuppressant, mycophenolate mofetil (MMF), increased the misincorporation of GCV in genomic DNA and mutations attributed to GCVsig in cell lines and organoids. Conclusions In summary, GCV can cause a diverse range of cancers. Its mutagenicity may be potentiated by other therapies, such as mycophenolate, commonly co-prescribed with GCV for post-transplant patients. Further investigation of the optimal use of these drugs could help reduce GCV-associated mutagenesis in post-transplant patients.

Abstract Microneedles, especially hollow microneedles (HMNs), play an important role in drug delivery, but most of the current HMNs are manufactured based on silicon microfabrication (lithography, etching, etc.), which are slightly conservative due to the lack of low‐cost, batch‐scale and customized preparation approach, especially for the HMNs with flexible substrate. For the first time, we propose the use of a high‐precision 3D printed master mold followed by a dual‐molding process for the preparation of HMNs with different shapes, heights, and inner and outer diameters to satisfy different drug delivery needs. The 3D printed master mold and negative mold can be reused, thereby significantly reducing the cost. HMNs are based on biocompatible materials, such as heat‐curing polymers or light‐curing resins. The thickness and rigidity/flexibility characteristics of the substrate can be customized for different applications. The drug delivery efficiency of the fabricated HMNs was verified by the in situ treatment of psoriasis on the backs of mice, which required only a 0.1‐fold oral dose to achieve similar efficacy, and the associated side effects and drug toxicity were reduced. Thus, this dual‐molding process can reinvigorate HMNs development.

Highlights Polyimide-based flexible microneedle array (PI-MNA) electrodes realize high electrical/mechanical performance and are compatible with wearable wireless recording systems. The normalized electrode–skin interface impedance (EII) of the PI-MNA electrodes reaches 0.98 kΩ cm2 at 1 kHz and 1.50 kΩ cm2 at 10 Hz, approximately 1/250 of clinical standard electrodes. This is the first report on the clinical study of microneedle electrodes. The PI-MNA electrodes are applied to clinical long-term continuous monitoring for polysomnography. Abstract Microneedle array (MNA) electrodes are an effective solution to achieve high-quality surface biopotential recording without the coordination of conductive gel and are thus very suitable for long-term wearable applications. Existing schemes are limited by flexibility, biosafety, and manufacturing costs, which create large barriers for wider applications. Here, we present a novel flexible MNA electrode that can simultaneously achieve flexibility of the substrate to fit a curved body surface, robustness of microneedles to penetrate the skin without fracture, and a simplified process to allow mass production. The compatibility with wearable wireless systems and the short preparation time of the electrodes significantly improves the comfort and convenience of electrophysiological recording. The normalized electrode–skin contact impedance reaches 0.98 kΩ cm2 at 1 kHz and 1.50 kΩ cm2 at 10 Hz, a record low value compared to previous reports and approximately 1/250 of the standard electrodes. The morphology, biosafety, and electrical/mechanical properties are fully characterized, and wearable recordings with a high signal-to-noise ratio and low motion artifacts are realized. The first reported clinical study of microneedle electrodes for surface electrophysiological monitoring was conducted in tens of healthy and sleep-disordered subjects with 44 nights of recording (over 8 h per night), providing substantial evidence that the electrodes can be leveraged to substitute for clinical standard electrodes.

Carbon dioxide capture, EOR-utilization and storage (CCUS-EOR) are the most practical and feasible large-scale carbon reduction technologies, and also the key technologies to greatly improve the recovery of low-permeability oil fields. This paper sorts out the main course of CCUS-EOR technological development abroad and its industrialization progress. The progress of CCUS-EOR technological research and field tests in China are summarized, the development status, problems and challenges of the entire industry chain of CO2 capture, transportation, oil displacement, and storage are analyzed. The results show a huge potential of the large-scale application of CCUS-EOR in China in terms of carbon emission reduction and oil production increase. At present, CCUS-EOR in China is in a critical stage of development, from field pilot tests to industrialization. Aiming at the feature of continental sedimentary oil and gas reservoirs in China, and giving full play to the advantages of the abundant reserves for CO2 flooding, huge underground storage space, surface infrastructure, and wide distribution of wellbore injection channels, by cooperating with carbon emission enterprises, critical technological research and demonstration project construction should be accelerated, including the capture of low-concentration CO2 at low-cost and on large-scale, supercritical CO2 long-distance transportation, greatly enhancing oil recovery and storage rate, and CO2 large-scale and safe storage. CCUS-EOR theoretical and technical standard system should be constructed for the whole industrial chain to support and promote the industrial scale application, leading the rapid and profitable development of CCUS-EOR emerging industrial chain with innovation.

Importance: Brain–computer interface (BCI) decodes and converts brain signals into machine instructions to interoperate with the external world. However, limited by the implantation risks of invasive BCIs and the operational complexity of conventional noninvasive BCIs, applications of BCIs are mainly used in laboratory or clinical environments, which are not conducive to the daily use of BCI devices. With the increasing demand for intelligent medical care, the development of wearable BCI systems is necessary. Highlights: Based on the scalp-electroencephalogram (EEG), forehead-EEG, and ear-EEG, the state-of-the-art wearable BCI devices for disease management and patient assistance are reviewed. This paper focuses on the EEG acquisition equipment of the novel wearable BCI devices and summarizes the development direction of wearable EEG-based BCI devices. Conclusions: BCI devices play an essential role in the medical field. This review briefly summarizes novel wearable EEG-based BCIs applied in the medical field and the latest progress in related technologies, emphasizing its potential to help doctors, patients, and caregivers better understand and utilize BCI devices.

In this paper, three studies on modal bridge expansion joints were conducted through experiments. The advantages and disadvantages of acceleration and fiber optic strain sensors in the tested modal expansion joints were compared. Secondly, the variation in the natural frequency of the modal bridge expansion joints at different concrete curing periods was investigated. Finally, the effect of damage on natural frequency in different parts (the center beam, the support bar, and concrete in the anchorage zone) of the modal bridge expansion joint was analyzed. For this purpose, three specimens were cast, each with six damage states. Manual methods damaged the specimens. An impact hammer was used to excite the corresponding parts of the different components. The results showed that the acceleration sensor is optimal for the modal bridge expansion joint test. The specimen’s natural frequency increased with the curing time’s growth. The natural frequency increased by 10 Hz from day 3 to day 28 of curing. With the gradual increase in damage, the natural frequencies of the center beam and support bar showed a gradual decreasing trend. The damage to the concrete in the anchorage zone caused less significant changes in the natural frequency, but the overall natural frequency still had a decreasing trend. The sensitivity of each frequency to the damage was different in different parts.

A novel method is proposed for the damage identification of modal bridge expansion joints (MBEJs) based on sound signals. Two modal bridge expansion joint specimens were fabricated to simulate healthy and damaged states. A microphone was used to collect the impact signals from different specimens. The wavelet packet energy ratio of the sound signal was used to identify the difference in specimen state. Firstly, the wavelet packet energy ratio was used to establish the feature vectors, which were reduced dimensionality using principal component analysis. Subsequently, a support vector data description model was established to detect the difference in the signals. The identification effects of three parameter optimization methods (particle swarm optimization, genetic algorithm optimization, and Bayesian optimization) were compared. The results showed that the wavelet packet energy ratio of sound signals could effectively distinguish the state of the support bar. The support vector data description of Bayesian optimization worked best, and the proposed method could successfully detect damage to the support bar of MBEJs with an accuracy of 99%.

Monitoring sleep conditions is of importance for sleep quality evaluation and sleep disease diagnosis. Accurate respiration detection provides key information about sleep conditions. Here, we propose a perforated temperature sensor that can be worn below the nasal cavity to monitor breath. The sensing system consists of two perforated temperature sensors, signal conditioning circuits, a transmission module, and a supporting analysis algorithm. The perforated structure effectively enhances the sensitivity of the system and shortens the response time. The sensor’s response time is 0.07 s in air and sensitivity is 1.4‰°C−1. The device can achieve a monitoring respiratory temperature range between normal room temperature and 40 °C. The simple and standard micromachining process ensures low cost and high reproducibility. We achieved the monitoring of different breathing patterns, such as normal breathing, panting, and apnea, which can be applied to sleep breath monitoring and exercise information recording.

Worldwide practices have proven that gas-assisted gravity drainage can obviously enhance oil recovery, and this technology can be especially effective for reservoirs with a thick formation and large inclination angle. For the successful implementation of this process, a key technology is the stable control of gas–oil interface during gas injection. For a detailed exploration of this technique, a three-stage permeable visual model was designed and manufactured, with permeability decreasing from top to bottom, thus, a reverse rhythm reservoir was effectively modeled. Then the experiment concerning CO2-assisted gravity drainage was carried out with the adoption of a self-developed micro visual displacement device. This study mainly focused on the micro migration law of gas–oil interface and the development effects of CO2-assisted gravity drainage. According to the experiments, CO2 fingering somewhat happens in the same permeable layer from the beginning of gas injection. However, phenomena of “wait” and “gas–oil interface self-adjustment” occur instead of flowing into the next layer when the injected CO2 reaches the boundary of the next lower permeability layer through the dominant channel. By the “gas–oil interface self-adjustment”, the injected CO2 first enters into the pores of the relative higher permeability layer to the greatest extent, and thus expands the sweep volume. Futhermore, in the process of CO2 injection, obvious gas channeling occurs in the low permeability layer directly connected to the outlet, resulting in low sweep efficiency and poor development effect. After connecting the core with lower permeability at the outlet, the development indexes of the model, such as the producing degree of the low permeability layer, the oil recovery before and after gas breakthrough, are significantly improved, and the recovery degrees of the medium permeability layer and the high permeability layer are also improved, and the overall recovery factor is increased by 12.38%. This “gas–oil interface self-adjustment” phenomenon is explained reasonably from the two scales of macroscopic flow resistance and microscopic capillary force. Finally, the enlightenments of the new phenomenon are expounded on the application of gas-assisted gravity drainage on site and the treatment of producers with gas breakthrough in gas injection development.

To explore the methodology for improving ultralow permeability reservoir recovery, cores of ultralow permeability reservoirs in China’s Ordos Basin were selected to study the dynamic imbibition micromechanism of crude oil in nanopore throat through core-flooding laboratory experiment and nuclear magnetic resonance (NMR) observation. In the meantime, the microimbibition characteristics and dynamic discharge of oil between matrix and fracture in partially closed boundary reservoirs were simulated to utmostly reflect the actual reservoir conditions. Our findings suggest that dynamic imbibition between fracture and matrix serves the core technology for improving the recovery of ultralow permeability reservoirs, while the main factors affecting dynamic imbibition efficiency include wettability, permeability, injection rate, fracture, water huff and puff cycles, and soaking time. Wettability, in particular, weighs the most, and imbibition can take place either on water-wet rocks or transformed oil-wet rocks with an imbibition agent added in during the waterflooding process. Meanwhile, the higher the permeability is in place, the greater the dynamic imbibition recovery might achieve. The experiments indicate that the dynamic imbibition recovery of a fractured core is 16.26% higher than that of a nonfractured core. Additionally, fractures can not only enhance imbibition recovery but also accelerate the occurrence of dynamic imbibition. The optimal water injection rate of dynamic imbibition is 0.1 mL/min; the reasonable huff and puff cycle of the ultralow permeability reservoirs tends to be two to three cycles; the optimal soaking time of ultralow permeability reservoir is speculated to be 30 days. Finally, the field practice shows that after Stimulated Reservoir Volume (SRV) and dynamic imbibition in 5 horizontal wells in An83 oilfield, there is a remarkable drop in water cut and a noticeable rise in oil production. This research underpins the significance of a dynamic imbibition effect in the development of ultralow permeability oilfield.

Current hydraulic fracture models are mainly based on elastic theories, which fail to give accurate prediction of fracture parameters in plasticity formation. This paper proposes a fluid–solid coupling model for fracture propagation in elastoplastic formations. The rock plastic deformation in the model satisfies the Mohr-Coulomb yield criterion and plastic strain increment theory. The extended finite-element method (XFEM) combined with the cohesive zone method (CZM) is used to solve the coupled model. The accuracy of the model is validated against existing models. The effects of stress difference, friction angle, and dilation angle on fracture shape (length, width), injection pressure, plastic deformation, induced stress, and pore pressure are investigated through the model. The results indicate that compared with elastic formation, fracture propagation in elastoplastic formation is more difficult, the breakdown pressure and extending pressure are greater, and fracture shape is wider and shorter. The plastic deformation causes the fracture tip to become blunt. Under the condition of high stress difference or low friction angle formation, it is prone to occur large plastic deformation zones and form wide and short fracture. Compared with friction angle, dilation angle is less sensitive to plastic deformation, fracture parameters, and fracture geometry. For the formation with high stress difference and friction angle, the effect of plasticity deformation on fracture propagation should not be ignored.

Objective: To explore the effect of fascia iliaca compartment block (FICB) in combination with ropivacaine on postoperative outcomes in elderly patients undergoing hip fracture (HF) repair. Methods: Retrospective analysis included data of 111 elderly patients who underwent HF surgery with FICB in Changxing County People's Hospital from October 2018 to October 2022. Observation group received 0.25% ropivacaine combined with FICB (n=52), and the control group was administered an intravenous injection of parecoxib sodium (n=59). Baseline characteristics of the patients, and indexes such as mean arterial pressure (MAP), heart rate (HR), and visual analogue scale (VAS) pain scores, were collected at one-, six-, 12- and 24-hours past surgery, both at rest and after passive movement. Results: VAS scores, MAP and HR at rest and after a passive movement in both groups were comparable before the surgery. VAS sores were significantly lower in the observation group at one-, six-, 12- and 24-hours after the surgery (P<0.05). Postoperative MAP in the observation group (80.83 ± 8.31) was significantly lower compared to the control group (95.29 ± 8.45 (t = -9.0659, p < 0.0001). Similarly, HR of the observation group was significantly lower one-hour post-surgery both at rest (t = -2.0468, p = 0.0431) and after passive movement (t = -6.0625, p < 0.001), and at all subsequent time intervals after the passive movement (P<0.05). Conclusions: Ropivacaine combined with FICB was associated with improved post-operative outcomes such as lower post-surgery VAS scores, MAP and HR compared to the intravenous injection of parecoxib sodium. [ABSTRACT FROM AUTHOR]

Objective: To compare the analgesic effects and incidence of postoperative adverse events after the erector spine plane (ESP) and transversus abdominis plane (TAP) blocks in patients undergoing laparoscopic cholecystectomy (LC). Methods: In this retrospective observational study, clinical data of 103 patients undergoing LC in Changxing County People's Hospital from October 2020 to October 2022 were retrospectively reviewed, and the patients were divided into ESP-group (n=56) and TAP-group (n=57) based on the block method. The operation time, the change of visual analogue scale (VAS) score of static (sVAS) and dynamic (dVAS) pain after operation, the patient-controlled dose, and the remedial analgesic dose at 24 hours after the operation were compared between the two groups. The occurrence of postoperative adverse reactions in both groups was recorded. Results: The dVAS scores of the ESP-group at one hour, three hours, six hours, and 12 hours after the operation were lower than those of the TAP-group (P<0.05). The patient-controlled dose and remedial analgesia dose of the ESPgroup were significantly lower than those of the TAP-group (P<0.05). There was no difference in the incidence of postoperative nausea and vomiting between the two groups (P>0.05). Conclusions: ESP block and TAP block in LC patients have similar operation time and incidence of postoperative adverse events such as nausea and vomiting. However, short-term postoperative analgesic effect of ESP block is superior to TAP and requires a lower dose of analgesia. [ABSTRACT FROM AUTHOR]

Abstract Diabetes mellitus affected more than 500 million of people globally, with an annual mortality of 1.5 million directly attributable to diabetic complications. Oxidative stress, in particularly in post-prandial state, plays a vital role in the pathogenesis of the diabetic complications. However, oxidative status marker is generally poorly characterized and their mechanisms of action are not well understood. In this work, we proposed a new framework for deep characterization of oxidative stress in erythrocytes (and in urine) using home-built micro-scale NMR system. The dynamic of post-prandial oxidative status (against a wide variety of nutritional load) in individual was assessed based on the proposed oxidative status of the red blood cells, with respect to the traditional risk-factors such as urinary isoprostane, reveals new insights into our understanding of diabetes. This new method can be potentially important in drafting guidelines for sub-stratification of diabetes mellitus for clinical care and management.