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The prevalence of per- and polyfluoroalkyl substances (PFASs) in agricultural soils has raised concerns regarding the health risks associated with the consumption of PFAS-contaminated agricultural products. The present study investigated the uptake and translocation of nine PFASs in lettuce using a hydroponic setting. During the uptake experiments, long-chain PFASs (≥ C8) exhibited greater accumulations in lettuce roots, while short-chain PFASs (≤ C7) manifested preferential transport to the shoots. The average root concentration factors of PFASs were positively correlated with their log K

Microplastics are suspected to deliver hydrophobic organic chemicals (HOCs) to marine organisms as it has high sorption capacity towards HOCs. In this study, the roles of microplastics ingestion in the overall uptake of HOCs by fish and seabird were evaluated quantitatively using mass-balance models including their biological features and possible HOCs intake routes (i.e., air, seawater, food, and microplastics). HOCs having wide range of partitioning properties (n = 203) were chosen and the contribution of microplastics was compared with other intake routes and further visualized using 2-dimensional contour diagrams. For most of the non-additives (n = 170), the contribution of microplastics was observed to be negligible (

Permethrin is one of the most widely used active ingredients in spray-type home insecticides. However, indoor permethrin exposure resulting from the use of home insecticides is not well-characterized, as measured permethrin concentrations in indoor environmental and biological media with a known application rate are scarce. We conducted an intervention study with four participants for seven days. We conducted personal air monitoring and collected 24-h urine samples in which we quantified time-weighted average (TWA) permethrin concentrations in indoor air (C

Interactions between microbes and microplastics are important as of emerging plastic loads in the global environment. Although diverse plastic additives are used in large amounts, there are very few studies on a quantitative comparison of plastisphere on plastics with different plastic additives. We studied the effects of two widely used UV stabilizers (benzotriazole-type UV-327 and benzophenone-type UV-531 were selected based on their persistence and toxicity) in low-density polyethylene (LDPE) on freshwater microbes. This is the first study on the sole effects of UV stabilizers used as plastic additives on freshwater in situ plastisphere biofilm development. Confocal laser scanning microscopy, assisted with proper differentiating fluorochromes and threshold-based 3D segmentation of data, was used to visualize and quantify biofilm. On the first week of biofilm growth, there was very little biovolume and a negligible amount of phototrophs on pristine LDPE contrasting other substrates. Biovolumes were significantly higher on LDPE with UV stabilizers (up to 159% higher than pristine LDPE), although the biomass was mostly dead due to toxicity (100% higher dead biovolume than live biovolume in LDPE with UV stabilizers). After the fourth week, marginally higher biovolumes along with a revival of the biomass on LDPE with UV stabilizers were observed. The ability to induce microorganismic intracellular reactive oxygen species by UV stabilizers was detected, which may stimulate biofilm growth during the primary phase of biofilm development. Atomic force microscopy analysis denoted that LDPE with UV stabilizers exhibit considerably stronger adhesion force than pristine LDPE. These observations suggest that UV stabilizers can foster the early attachment of microbes to microplastics while killing the surface contacting layer. An alive upper layer of microbes can get developed on the dead biofilm without much disruption due to the toxicity of UV stabilizers. This occurrence can eventually boost the early development of biofilms on plastics.

Microplastic particles have recently attracted much attention owing to their potential adverse effects on marine and terrestrial environments. Although several studies have been conducted on this topic, one of the prominent existing challenges is developing analytical methods to precisely characterize isolated microplastics. Specifically, a systematic method that determines both the size and shape of irregular micron-sized particles is required because conventional optical methods provide only two-dimensional images of microplastics and cannot easily handle cases of tilting or aggregation of particles. In this study, we demonstrate that previously developed microfluidic technologies can be successfully applied to measure the size and shape of oblate microparticles utilizing viscoelastic particle focusing. Furthermore, this technique is also applicable for irregular microplastic fragments that are predominantly found in environmental samples.

Although hydrophobic ultraviolet (UV) stabilizers are an emerging environmental concern because of their widespread occurrence, persistence, and bioaccumulation potential, experimental values of their partitioning properties required for risk assessment are scarce. In this study, n-octanol-water partition (Kow) and lipid–water partition constants (Klipw), which are key parameters for environmental risk assessment, were experimentally determined for five selected hydrophobic UV stabilizers (UV326, UV327, UV328, UV329, and UV531) based on third-phase partitioning among polydimethylsiloxane (PDMS), water, and n-octanol/lipid. The partition constants between PDMS and water (KPDMSw), obtained using the dynamic permeation method were used to derive Kow and Klipw. The obtained log Kow and log Klipw values were in the ranges of 7.08–7.94 and 7.50–8.34, respectively, indicating that the UV stabilizers exhibited a high bioaccumulation potential in aquatic environments. The experimental Kow and Klipw values obtained in this study provide valuable information for the evaluation of the fate, distribution, bioavailability, and toxicity of the UV stabilizers in aquatic environments.

Micro- and nanoplastics (MNPs) are present in almost all environmental compartments. Terrestrial soils are major environmental reservoirs for MNPs, but the ecotoxicological effects of MNPs on terrestrial biota remain relatively understudied. In this review, we collated findings of previous research on the uptake and impact of MNPs in terrestrial organisms, including flora, fauna, and human beings. Terrestrial plants can take up MNPs via the roots or leaves and translocate them to other parts. MNPs have been detected in the gastrointestinal tracts or feces of many terrestrial animals, including some high trophic-level predators, indicating the incidence of direct ingestion or trophic transfer of MNPs. The presence of MNPs in food items and human feces combines to verify human intake of MNPs via the dietary pathway. Exposure to MNPs can cause diverse effects on terrestrial organisms, including alterations in growth performance, oxidative stress, metabolic disturbance, cytotoxicity, genotoxicity, and mortality. The biological internalization and impact of MNPs are influenced by the physicochemical properties of MNPs (e.g., particle size, polymer type, surface chemistry, and exposure concentrations) and the physiology of the species. MNPs can also affect the bioavailability of co-occurring intrinsic or extrinsic contaminants to terrestrial biota, but their specific role is under dispute. Finally, we underlined the current research gaps and proposed several priorities for future studies.

A novel method for the volumetric detection of microplastics in various environmental (soil, water) and food (fish, meat) matrices was developed. The method is based on the Nile Red staining of microplastics while eliminating probable interference by other organic polymers such as lignin, chitin, cellulosic materials, and other organic substances using a mixture of three water-based dyes (Calcofluor White, Evans Blue, and 4,6-diamidino-2-phenylindole [DAPI]). The excitation/emission 'sweet spot' was determined for water based blue dyes to detect them in a single channel for effective elimination of probable contaminations. Detection of microplastic particles using the Nile Red method was validated by comparing with traditional detection of microplastics via Fourier transform infrared spectroscopy (FTIR). Volumetric measurements of the microplastics present in environmental samples were made possible using Z-stack confocal microscopy images backed by threshold-based 3D segmentation. Regularly shaped microplastic materials were used to validate the volumetric measurement method. The proposed volumetric determination method will be very useful for screening microplastics in diverse media and improving the prevailing method using FTIR.

Large amounts of plasticizers, such as di(2-ethylhexyl) phthalate (DEHP) and dioctyl terephthalate (DOTP), are added to various polyvinyl chloride (PVC) products. To assess the human exposure to these plasticizers on using PVC products, it is important to know their migration rate. However, conventional migration tests conducted at a fixed time and temperature are often insufficient for determining possible variations in migration rates with respect to time, temperature, and sebum layer. In this study, the migration rates of DEHP and DOTP from five PVC products were measured using a polydimethylsiloxane (PDMS) sampler at different times and temperatures, in the presence and absence of artificial sebum. Although the migrated mass of the plasticizers increased over time, the average migration rate decreased. The average migration rates increased with increasing temperature and in the presence of an artificial sebum layer between the product and the PDMS sampler. When the artificial sebum layer was added, the average migration rate increased considerably by a factor of 1.5-14, suggesting that sebum should be considered to avoid the underestimation of dermal exposure to highly hydrophobic plasticizers, such as DEHP and DOTP. Based on the measured values, a conceptual analysis was conducted to quantitatively assess the difference in the migration rate of plasticizers caused by the difference between the time set for the migration test and the exposure time when the product is used. To reduce uncertainties and the potential underestimation of dermal exposure, an appropriate time for the experiment should be set to simulate the exposure scenario of a given product.

Cancer risk can be associated with exposure to polycyclic aromatic hydrocarbons (PAHs) in playground dust and soil. This study investigated the profiles and sources of PAHs from poured rubber-surfaced playground dust and uncovered playground surface soil, by applying an ex-situ equilibrium passive sampling technique. Surface dust and soil samples were collected from 15 different playgrounds in Seoul, Republic of Korea. The total 16 EPA PAHs concentrations in surface dust and soil varied from 198 to 919 μg kg−1 dw and 68–169 μg kg−1 dw, respectively. 4- to 6-ring PAHs were dominant, accounting for approximately 53.8%–94.5% of the total PAHs in surface dust and soil. The diagnostic ratios and principal component analysis suggested that a mixed coal combustion and vehicular emission was likely the main source of PAHs in the surface dust and soil. The higher total organic carbon content can explain the higher PAH accumulation and lower fugacities of PAHs. The fugacity comparison of phenanthrene and pyrene in dust, soil, air, and playground surface material indicated that atmospheric deposition is the main source of PAHs in the dust and soil on rubber-surfaced and uncovered surfaced playgrounds. This study contributes to the understanding of PAHs sources in dust and soil samples in children's playground and helps policymaker determine the right contamination sources for risk management.

Polyhexamethylene guanidine (PHMG) is a cationic antimicrobial oligomer that has been used prevalently over the past few decades. However, due to the lack of inhalation toxicity assessment of PHMG, it has caused severe health damage, including fatal lung fibrosis, after being used as one of the major active ingredients of humidifier disinfectants in Korea. Because the first step of the entry of PHMG into airway is its association with cell membranes, the distribution of PHMG between lipid membranes and water is very important to know the depositional flux in the respiratory systems and related toxic mechanisms. We developed a quantitative method to determine the distribution constant (Klipw) of PHMG between solid supported lipid membranes and water and evaluated the effects of lipid membrane compositions on the Klipw of PHMG. PHMG accumulated into anionic lipid membranes rapidly compared to into cationic or zwitterionic lipid membranes, suggesting fast adsorption of PHMG onto anionic lipid head groups. Klipw values with anionic/zwitterionic lipid mixtures were higher than Klipw values with anionic lipids only, potentially due to the later phase separation after preferential interaction between PHMG and anionic lipids in lipid mixtures. In addition, Klipw values increased with increasing single acyl chain lipid content in unsaturated lipids and decreasing cholesterol content. These results imply that changes in lipid spontaneous curvature and lipid bilayer packing density also affect the membrane distribution of PHMG.

In the standard ISO soil toxicity test using Collembola, adult survival and juvenile production are the only endpoints that can be attainable. The information on egg production and egg hatching cannot be investigated in the ISO test. To overcome this limitation, in this study, the effects of teflubenzuron on life history parameters of Yuukianura szeptyckii (Collembola) were investigated with a compressed soil test. Teflubenzuron is an insect growth regulator and has a negative effect on egg production, and egg hatching process of arthropods. LC50 decreased with increases in exposure period from 6.97 mg/kg in the third week to 3.60 mg/kg in the fourth week. The EC50 for egg and juvenile production was 0.57 mg/kg and 0.26 mg/kg, respectively. The hatching rate decreased significantly from 46 to 7% as the concentration increased from 0.25 to 1.00 mg/kg, respectively, and the molting frequency was significantly affected only at > 4 mg/kg. The toxic contribution rate (TCR) was defined as the ratio of juvenile production at an exposure concentration compared with the control, and a simple life history model was developed for TCR estimations. At the lower concentrations ( 2.0 mg/kg). The contribution of egg production reduction remained relatively constant. Since collembolan populations in the soil can be composed of various developmental stages, the differences in the sensitivity to chemicals depending on the developmental stages should be included in the assessment of the toxic impact on soil ecosystems.

Children can get affected by polycyclic aromatic hydrocarbons (PAHs) while they interact with play area soil/rubber surfacing and exposed to PAHs by dermal contact, inhalation and hand-to-mouth activity. A comparative study has been conducted on PAHs profiling and probable cancer risk of children from PAHs present in uncovered playground surface soil and poured rubber surfaced playground dust. Surface soil and dust samples have been collected from 14 different children parks around the Korea University campus, Seoul, Republic of Korea. Concentrations of 16 PAHs in the soils/dust were found to be in a range of 2.82–57.93 μg g−1. Profiling of the PAHs from the playground soils/dust reveals 3-ring PAHs are dominating with 79.9% of total PAHs content, on an average. The diagnostic ratio analysis confirms that vehicular exhaust and fossil fuel burning are likely the main sources of high molecular weight carcinogenic PAHs, whereas low molecular weight PAHs have pyrogenic origin. The probabilistic health risk assessment using Monte Carlo simulations for the estimation of the 95% cancer risk exposed to the PAHs from the surfaced playgrounds shows a little higher value than the USEPA safety standard (1.3 × 10−5). Sensitivity analysis revealed exposure duration and relative skin adherence factor for soil as the most influential parameters of the assessment. Noticeably, cancer risk is approximately 10 times higher in poured rubber surfaced playgrounds than in uncovered soil playgrounds.

The settling of microplastics (MPs) is crucial for their removal from municipal wastewater treatment plants (WWTPs) and sedimentation in static waterbodies, where they can accumulate in bottom sediments. Biofilm formation on MPs enhances their aggregation with other particles, thereby changing their density and size and altering their settling rates. However, only a few studies have investigated the settling of MPs of different sizes and materials. Specifically, the settling of small-sized MPs (150 μm) has been poorly documented. In this study, cylindrical and fragmented particles of four polymer types (high-density polyethylene (HDPE), polypropylene (PP), polystyrene (PS), and poly(ethylene terephthalate) (PET)) were used to investigate the settling or floating of reference MPs (20-130 μm) in a custom-made column that simulated a primary sedimentation tank in a typical WWTP before and after incubation in wastewater influent. The settling velocity of the reference MP particles was strongly influenced by the particle size and density. The settled fractions of all the cylindrical reference MPs increased significantly (up to 5 times) due to biofilm formation at overflow velocities of 0.15, 0.26, and 0.40 mm s

Various chlorine-based disinfectants are being used during the COVID-19 pandemic, however, only a few studies on exposure to harmful gases resulting from the use of these disinfectants exist. Previously, we developed a personal passive air sampler (PPAS) to estimate the exposure level to chlorine gas while using chlorinated disinfectants. Herein, we investigated the color development of the passive sampler corresponding to chlorine exposure concentration and time, which allows the general population to easily estimate their gas exposure levels. The uptake and reaction rate of PPAS are also explained, and the maximum capacity of the sampler was determined as 1.8 mol of chlorine per unit volume (m3) of the passive sampler. Additionally, the effects of disinfectant types on the gas exposure level were successfully assessed using passive samplers deployed in a closed chamber. It is noteworthy that the same level of chlorine gas is generated from liquid household bleach regardless of dilution ratios, and we confirmed that the chlorine gas can diffuse out from a gel-type disinfectant. Considering that this PPAS reflects reactive gas removal, individual working patterns, and environmental conditions, this sampler can be successfully used to estimate personal exposure levels of chlorinated gases generated from disinfectants.

Over the decades, guanidine-based oligomer groups have been one of the most widely used antimicrobial agents. Reportedly, these cationic oligomers cause serious damage to microorganisms but have low toxicity to humans. However, public concerns regarding the guanidine group have rapidly grown after the fatal misuse of these oligomers as humidifier disinfectants, which resulted in thousands of fatalities in South Korea. Herein, we investigated liposome leakage and cellular permeability changes caused by polyhexamethylene guanidine (PHMG) and polyhexamethylene biguanide (PHMB), both representative guanidine-based oligomers. The leakage of zwitterionic liposomes, induced by cationic oligomers, was more extensive than that of negative liposomes, indicating that oligomer adsorption onto lipid head groups via electrostatic interaction cannot fully explain the induced lipid membrane damage. Furthermore, lipid packing parameters, including intrinsic curvature, cholesterol content, and lipid phases, affected liposome leakage, particularly for PHMG. Cellular permeability tests were performed using an A549 cell monolayer model and a respiratory 3D tissue model, revealing that PHMG and PHMB damaged cell membranes and reduced cell barrier function. Furthermore, liposome leakage induced by PHMG and PHMB was higher in human lung surfactant-mimicking liposomes than that observed in Escherichia coli-mimicking liposomes. These results indicated that human cells are susceptible to guanidine-based oligomers. Considering that the interaction of oligomers and cell membranes is a major mechanism of toxicity initiation, this study provides crucial insights into the action of these disinfectants on mammalian cells.

(1) Background: Mathematical exposure modeling of volatile organic compounds (VOCs) in consumer spray products mostly assumes instantaneous mixing in a room. This well-mixed assumption may result in the uncertainty of exposure estimation in terms of spatial resolution. As the inhalation exposure to chemicals from consumer spray products may depend on the spatial heterogeneity, the degree of uncertainty of a well-mixed assumption should be evaluated under specific exposure scenarios. (2) Methods: A room for simulation was divided into eight compartments to simulate inhalation exposure to an ethanol trigger and a propellant product. Real-time measurements of the atmospheric concentration in a room-sized chamber by proton transfer reaction mass spectrometry were compared with mathematical modeling to evaluate the non-homogeneous distribution of chemicals after their application. (3) Results: The well-mixed model overestimated short-term exposure, particularly under the trigger spray scenario. The uncertainty regarding the different chemical proportions in the trigger did not significantly vary in this study. (4) Conclusions: Inhalation exposure to aerosol generating sprays should consider the spatial uncertainty in terms of the estimation of short-term exposure.

Abundant use of plastic materials has increased the amount of microplastics (MPs) and related hazardous chemicals in the marine environment. Hexabromocyclododecanes (HBCDs), brominated flame retardants added to expanded polystyrene (EPS), have been detected in biotic and abiotic samples. In this study, the partition constants of HBCDs between plastics and seawater (KPsw) were determined. Fugacities of HBCDs in EPS, seawater, sediment, and mussels were obtained to determine the directions of the diffusive flux. The fugacities in EPS (fEPS) were greater than those in seawater (fsw), sediment (fsed), and mussels (fswmussel-EPS and fmussel-rock) by three orders of magnitude, indicating that EPS plastics are a significant source of HBCDs. The fmussel-rock of α-HBCD in rock mussels was greater than fsw by factors of 1.7, whereas the fmussel-rock of γ-HBCD was smaller than fsw by factors of 16, indicating the bioisomerization from γ-to α-HBCD. The relatively constant concentration ratio of β-HBCD to the total HBCDs indicated that β-HBCD is a sufficient tracer for determining the diffusive flux. The fsed values of HBCDs were greater than fsw by factors of 17–28, implying a probable advective vertical flow of HBCDs from the EPS plastics, which requires further investigation.

This study aimed to quantify both chloromethylisothiazolinone (CMIT) and methylisothiazolinone (MIT) dissolved in different product brands and to characterize the exposure to these chemicals among humidifier disinfectant-associated lung injury (HDLI) patients. Both CMIT and MIT dissolved in different humidifier disinfectant (HD) products were quantified using gas chromatography&ndash, mass spectrometry. The inhalation level of CMIT and MIT was estimated based on HD-associated factors as reported by HDLI patients. A total of eleven HD products marketed until the end of 2011 were found to contain CMIT and/or MIT. The level of combined CMIT and/or MIT dissolved in these HD products ranged from 12 to 353 ppm. The level varied among HD products and the year of manufacture. The average inhalation levels were estimated to be 7.5, 4.1, and 3.2 &mu, g/m3 for the definite, probable, and possible groups, respectively. If probable and possible groups were collapsed together, the inhalation level of the collapsed group was significantly different from that of the definite group (p &lt, 0.001). All HDLI patients responded as having used HD not only while sleeping, but also as having a humidifier treated with HD within close proximity every day in insufficiently ventilated spaces. These HD use characteristics of patients may be directly/indirectly linked to the HDLI development.

Plasticizers are added to diverse consumer products including children’s products. Owing to their potential for endocrine disruption, the use of phthalate plasticizers is restricted in many children’s products. In this study, exposure to five phthalate esters (dibutylphthalate, di(2-ethylhexyl) phthalate (DEHP), diethyl phthalate, di-isobutyl phthalate, and diisononyl phthalate (DINP)) and an alternative (di-ethylhexyl adipate) was assessed by the use of children’s products based on chemical analysis of 3345 products purchased during 2017 and 2019 in Korea. Plasticizers were found above the detection limits in 387 products, and DEHP and DINP were the two most predominantly detected plasticizers. Deterministic and probabilistic estimation of the margin of exposure at a screening level revealed that the use of children’s products might be an important risk factor. However, it is also highly likely that the exposure could be overestimated, because the migration rate was estimated based solely on the content of plasticizers in children’s products. Chemical migration is a key process determining the absorption of plasticizers from products, thus, further refinements in experimental determination or model estimation of the migration rate are required.

With an increasing use of indoor disinfectants such as chlorine (Cl2 ) and hypochlorous acid, a convenient sampler for estimating exposure to oxidants, such as effective chlorine, is necessary. Here, we developed a personal passive air sampler (PPAS) composed of a redox dye, o-dianisidine, in a polydimethylsiloxane (PDMS) sheet. o-Dianisidine readily reacts with gaseous oxidants generated by bleach usage, and its color changes as the reaction progresses; hence, personal exposure to effective chlorine could be easily detected by the naked eye, while cumulative exposure could be determined by measuring concentrations of o-dianisidine reacting with it. The PPAS was calibrated, and a sampling rate of 0.00253 m3 /h was obtained using a small test chamber. The PPAS was tested with the help of ten volunteers whose personal exposure to Cl2 -equivalent gas was estimated after bathrooms were cleaned using spray and liquid-type household disinfection products, and the accumulated exposure-gas concentrations ranged from 69 to 408 ppbv and 148 to 435 ppbv, respectively. These PPAS-derived exposure concentrations were approximately two orders lower than those estimated using ConsExpo, suggesting a significant overestimation by prevailing screening models, possibly due to the ignorance of transformation reactions.

Human exposure to microplastics contained in food has become a significant concern owing to the increasing accumulation of microplastics in the environment. In this paper, we summarize the presence of microplastics in food and the analytical methods used for isolation and identification of microplastics. Although a large number of studies on seafood such as fish and shellfish exist, estimating the overall human exposure to microplastics via food consumption is difficult owing to the lack of studies on other food items. Analytical methods still need to be optimized for appropriate recovery of microplastics in various food matrices, rendering a quantitative comparison of different studies challenging. In addition, microplastics could be added or removed from ingredients during processing or cooking. Thus, research on processed food is crucial to estimate the contribution of food to overall human microplastic consumption and to mitigate this exposure in the future.

A low-density polyethylene (LDPE) degrading bacterial strain (ATKU1) was isolated (99.86% similar with Bacillus siamensis KCTC 13613T) from a plastic dumping site to study interactions between microplastics (

Surfactants, including quaternary ammonium compounds, are widely used in daily life as part of consumer chemical products and, more recently, in the shale oil industry. Because of their unique amphiphilic properties, surfactants form micelles at concentrations above a certain threshold known as the critical micelle concentration (CMC). A previous electrospray ionization mass spectrometry studies conducted by Siuzdak et al. and others presented indirect evidence regarding micelle formation. Herein, we have used liquid chromatography-tandem mass spectrometry to explore how such micelle formations affect the quantitative analysis of surfactants. Results reveal abnormal behaviors in the calibration plots of a few selected anionic and cationic surfactants, such as sodium decyl sulfate (SDeS), sodium dodecyl sulfate (SDS), myristyltrimethylammonium bromide (MTAB), and benzyldimethyloctadecylammonium chloride (BAC-18). At concentrations close to the respective CMCs of these surfactants, the calibration plot for MTAB flattened, whereas the slopes of the calibration plots for SDeS, SDS, and BAC-18 suddenly changed. These abnormal behaviors can be related to micelle formation. From a practical perspective, the above observations suggest that in the quantitative analysis of surfactants, high micelle concentrations close to the CMC should be avoided to obtain accurate surfactant measurements.

One of the most important concerns about marine microplastics is their role in delivery of chemical contaminants to biota. The contribution of microplastic ingestion to the overall uptake of five hydrophobic organic chemicals (HOCs) [α-, β-, and γ-hexachlorocyclohexanes (HCHs), pentachlorobenzene (PeCB), and hexachlorobenzene (HeCB)] by fish is evaluated in this study. Partition coefficients of all five HOCs between surfactant micelles and simulated intestinal fluid (SIF), as well as between protein and SIF, were experimentally determined. Desorption of model HOCs from a polyethylene film into an artificial gut solution was measured to estimate the fraction of HOCs that can be absorbed from microplastics during their gut retention time. Monte-Carlo simulation (n = 100,000) showed that the uptake via microplastic ingestion will be negligible for HCHs as compared to uptake via other exposure routes, water ventilation and food ingestion. On the other hand, microplastic ingestion might increase the total uptake rate of PeCB and HeCB due to their accelerated desorption from microplastics into the artificial gut solution under the model scenario, assuming an extremely high intake of microplastics. However, the steady-state bioaccumulation factor was predicted to decrease with increasing ingestion of microplastics, showing a dilution effect by microplastic ingestion. Results indicate that HOCs that are close to be at phase equilibrium between microplastics and environmental media are not likely to be further accumulated via ingestion of microplastics; this is true even for cases, where ingestion of microplastics contributes significantly to the total uptake of HOCs. Therefore, future studies need to focus on hydrophobic plastic additives that may exist in microplastics at a concentration higher than their equilibrium concentration with water.

In Korea, biocides, which were used for sterilizing household humidifiers, caused a large number of casualties. The biocides used were either guanidine-containing oligomers, such as polyhexamethylene guanidine (PHMG), or thiazolinone-based small molecules such as methylisothiazolinone/methylchloroisothiazolinone (MIT/CMIT). For the guanidine-containing oligomers, a quantitative analysis method has not yet been developed, as the quantification of the oligomers is very challenging due to their polydispersed molecular weight distribution. In this paper, we describe a new MALDI-TOF mass spectrometry (MS)-based quantitative analysis method for the PHMG oligomers contained in wet wipe products. Quantification was made using internal standard 13C labeled PHMG oligomers that were custom synthesized. To get rid of matrix molecules, for example, polyethylene glycols (PEGs), included in the wet wipes, solid phase extraction (SPE) with an MCX SPE cartridge was carried out. In addition, an ionic liquid matrix (ILM) of 1-methylimadazolium α-cyano-4-hydroxycinnamate (1-MeIm-CHCA) was used to improve the reproducibility of the MALDI-TOF MS measurements. This new quantitative analysis method was validated, and good linearity was obtained in the calibration plot. This approach is expected to be equally applicable to other guanidine-containing oligomers, such as polyhexamethylene biguanide (PHMB) and oligo-(2-(2-ethoxy)-ethoxyethyl)-guanidinium-chloride (PGH) as well as to other consumer products.