1. The selection of a standard STR panel for DNA profiling of the African elephant (Loxodonta africana) in Kenya
- Author
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Mercy W. Mwaniki, Francis Gakuya, Julius Kinuthia, Amos Alakonya, Shadrack Muya, Cindy Kim Harper, Charles Kimwele, Erastus Gatebe, and Anne W. T. Muigai
- Subjects
education.field_of_study ,Population ,Zoology ,Poaching ,Biology ,African elephant ,DNA profiling ,biology.animal ,Multiplex polymerase chain reaction ,Genetics ,Microsatellite ,education ,Genotyping ,Ecology, Evolution, Behavior and Systematics ,Selection (genetic algorithm) - Abstract
The African elephant (Loxodonta africana) population in Kenya is declining at an alarming rate due to habitat destruction, human-elephant conflicts and the cur- rent escalation of poaching for ivory. This study estab- lished a standard protocol for forensic analysis of L. africana and their products such as ivory. Three multiplex PCR panels consisting of 17 Short Tandem Repeat markers were selected from 40 markers using bioinformatics tools, amplification and polymorphism. Genotyping was suc- cessful and reproducible. This method is efficient, accurate and cost effective and has potential for application in conservation of L. africana.
- Published
- 2014
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