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The selection of a standard STR panel for DNA profiling of the African elephant (Loxodonta africana) in Kenya

Authors :
Mercy W. Mwaniki
Francis Gakuya
Julius Kinuthia
Amos Alakonya
Shadrack Muya
Cindy Kim Harper
Charles Kimwele
Erastus Gatebe
Anne W. T. Muigai
Source :
Conservation Genetics Resources. 7:305-307
Publication Year :
2014
Publisher :
Springer Science and Business Media LLC, 2014.

Abstract

The African elephant (Loxodonta africana) population in Kenya is declining at an alarming rate due to habitat destruction, human-elephant conflicts and the cur- rent escalation of poaching for ivory. This study estab- lished a standard protocol for forensic analysis of L. africana and their products such as ivory. Three multiplex PCR panels consisting of 17 Short Tandem Repeat markers were selected from 40 markers using bioinformatics tools, amplification and polymorphism. Genotyping was suc- cessful and reproducible. This method is efficient, accurate and cost effective and has potential for application in conservation of L. africana.

Details

ISSN :
18777260 and 18777252
Volume :
7
Database :
OpenAIRE
Journal :
Conservation Genetics Resources
Accession number :
edsair.doi.dedup.....e1e72fc0d80c4cc5c0561bfacb2a42fa
Full Text :
https://doi.org/10.1007/s12686-014-0366-6