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The purpose of this preclinical study was to evaluate the influence of tamoxifen (TAM) on the peri-implant bone remodeling of osseointegrated titanium implants in ovariectomized female rats.Seventy-two female rats underwent bilateral ovariectomy 20 weeks before implants placement. One titanium implant was inserted in each tibia of the animals. Six weeks following the implant surgery, animals were randomly divided into two experimental groups (n = 36), which received either saline solution (SS) or tamoxifen citrate (TAM) via gavage until euthanasia. Euthanasia was performed at 30, 60, and 90 days after the first gavage. Assessments of bone to implant contact (BIC), bone ingrowth percentage (BIN), morphological description of cellular and tissue reactions, immunohistochemistry for the detection of bone morphogenetic protein 2/4 (BMP2/4), runt-related transcription factor 2 (RUNX-2), osteocalcin (OCN) and tartrate-resistant acid phosphatase (TRAP), and bone chemical composition through scanning electron microscopy with energy-dispersive x-ray spectroscopy were performed.Tamoxifen group presented higher BIC, higher BIN, higher RUNX-2 and OCN, lower TRAP-positive cells/mmTamoxifen enhanced the remodeling of the bone surrounding titanium implants in ovariectomized rats.

A recessão gengival é caracterizada como o deslocamento apical da margem gengival em relação à junção cemento-esmalte, com consequente exposição da superfície radicular podendo promover desconforto estético e funcional. O tratamento das recessões gengivais consiste em técnicas cirúrgicas de recobrimento radicular, geralmente associadas ao enxerto de tecido conjuntivo autógeno, contudo, para a sua obtenção existe a necessidade de um segundo sítio cirúrgico, aumentando a morbidade no pós-operatório do paciente. O propósito deste relato de caso é apresentar a matriz de colágeno suína associada ao retalho posicionado coronal como alternativa ao enxerto autógeno de tecido conjuntivo. Paciente do gênero feminino, 45 anos, compareceu ao atendimento na clínica de periodontia do Unifunec (Centro Universitário de Santa Fé do Sul), queixando-se da estética do sorriso devido a recessões gengivais dos dentes 22 a 24. Após a avaliação clínica, a paciente foi diagnosticada com recessão gengival Classe RT1 dos dentes 22 a 24. A paciente foi submetida a cirurgia de recobrimento radicular por meio da técnica do retalho posicionado coronal associado à matriz de colágeno suína. Foi realizado acompanhamento de 30 dias, 12 e 24 meses. Após acompanhamento dos referidos períodos, notou-se um recobrimento satisfatório da superfície radicular, ganho de tecido gengival em altura e espessura evidenciado clinicamente, ausência de recidiva e trazendo satisfação funcional e estética para o paciente. Conclui-se que a utilização da matriz de colágeno suína, reduziu a morbidade do paciente, promoveu ganho de espessura do tecido gengival na área receptora, favorecendo a estabilidade tecidual. PORCINE COLLAGEN MATRIX AND CORONALLY DISPLACED FLAP IN THE TREATMENT OF MULTIPLE RECESSIONS: A CASE REPORT ABSTRACT Gingival recession is characterized as the apical displacement of the gingival margin in relation to the cement enamel junction, with consequent exposure of the root surface, which may cause aesthetic and functional discomfort. The treatment of gingival recessions consists of surgical techniques for root coverage, which are usually associated with autogenous connective tissue grafting; however, in order to obtain it, a second surgical procedure is required, increasing the patient's postoperative morbidity. The aim of this case report is to present the porcine collagen matrix associated with the coronally positioned flap as an alternative to autogenous connective tissue grafting. A 45-year-old female patient came to the periodontics clinic of the Unifunec (University Center of Santa Fé do Sul) complaining about the aesthetics of her smile due to gingival recession on teeth 22 to 24. After clinical evaluation, the patient was diagnosed with Class RT1 gingival recession on teeth 22 to 24. The patient underwent root coverage surgery using the coronally positioned flap technique associated with porcine collagen matrix. Follow-up was performed for 30 days, 12 and 24 months. After follow-up of the mentioned periods, it was noted a satisfactory covering of the root surface, gain of gingival tissue in height and thickness clinically evidenced, absence of relapse and bringing functional and aesthetic satisfaction to the patient. It can be concluded that the use of porcine collagen matrix reduced the morbidity of the patient, promoted thickness gain of the gingival tissue in the recipient area, favoring tissue stability. Keywords: Gingival recession. Periodontics. Gingiva. Soft tissue management. Collagen matrix. Mucogingival surgery.

Objective The aim of this study was to evaluate the effectiveness of hand debridement (HD) versus ultrasonic dental scaler (UDS) for the treatment of experimental periodontitis (EP) in rats. Material and methods Thirty 3‐month‐old male rats were used. EP was induced around the mandibular first molars (right and left). Seven days after induction, the treatments with either HD (n = 30) or UDS (n = 30) were randomly performed in each molar. Euthanasia were performed at 7, 15, and 30 days after treatment. Histometric (percentage of bone in the furcation [PBF]), histopathological, and immunohistochemical (for detection of tartrate-resistant acid phosphatase [TRAP] and osteocalcin [OCN]). Parametric data (PBF and TRAP) was analyzed by One-way ANOVA followed by Tukey’s post-test. OCN was analyzed by Kruskal-Wallis followed by Student-Newman-Keuls post-test. The level of significance was 5%. Results Group HD presented higher PBF and lower TRAP-immunolabeling at 30 days as compared with UDS in the same period (p≤0.05). Group HD presented higher OCN immunolabeling at 30 days as compared with 7 and 15 days (p≤0.05). Persistent and exacerbated inflammatory process was observed in some specimens from group UDS at 30 days, as well as the bone trabeculae presented irregular contour, surrounded by many active osteoclasts. Conclusion Nonsurgical periodontal therapy with HD resulted in higher PBF and lower expression of TRAP as compared with UDS. Also, HD increased the expression of OCN over time.

Terapias coadjuvantes ao tratamento convencional da periodontite têm sido sugeridas para otimizar os resultados terapêuticos, sendo que o chá verde tem sido proposto por apresentar propriedades benéficas ao tratamento periodontal. Nesse contexto, o objetivo desta revisão integrativa foi avaliar se o uso do chá verde é eficaz no tratamento e controle da periodontite. Para isso, foi realizado um levantamento bibliográfico nas bases de dados PubMed, Lilacs e Cochrane Library, com os descritores green tea, periodontal disease, Camellia sinensis e periodontitis. Foram selecionados artigos na língua inglesa publicados nos últimos 10 anos em que o chá verde deveria ser obrigatoriamente empregado como uma conduta terapêutica e incluídos estudos clínicos randomizados controlados e revisões sistemáticas. Foram excluídos ensaios pré-clínicos, relato de caso clínico, série de relatos clínicos, estudos epidemiológicos, editoriais, opiniões de especialistas, estudo piloto, revisões de literatura, pesquisas com resultados inconcludentes e estudos in-vitro. A busca resultou em 450 artigos dos quais, após aplicação dos critérios de inclusão e exclusão, selecionaram-se 10 artigos, sendo 8 ensaios randomizados controlados e 2 revisões sistemáticas com meta-análise. 6 artigos avaliaram a efetividade do chá verde como colutório oral demonstrando redução da inflamação e do acúmulo de placa bacteriana, 3 estudos associaram o chá verde a raspagem e alisamento radicular, demonstrando diminuição da profundidade, sangramento a sondagem e redução do quadro inflamatório e 1 estudo avaliou a ingestão sistêmica, demonstrando redução na profundidade de sondagem e sangramento. Dessa forma, podemos concluir que o chá verde é eficaz como terapia coadjuvante no tratamento e controle da periodontite. Quando associado à raspagem e ao alisamento radicular e como colutório oral, apresentam-se resultados mais satisfatórios que na forma de ingestão oral. ASSESSMENT OF GREEN TEA (CAMELLIA SINENSIS) USED AS AN ADJUNCT TO TREATMENT AND CONTROL OF PERIODONTAL DISEASE: INTEGRATIVE REVIEW Adjunct therapies to conventional treatment for periodontitis have been suggested to optimize therapeutic results; green tea has been suggested because it presents beneficial properties for periodontal treatment. In this context, this integrative review aims at assessing the effectiveness of green tea for periodontitis treatment and control. Thus, bibliographic research was carried out in PubMed, LILACS, and Cochrane Library databases, using the words green tea, periodontal disease, Camelliasinensis, and periodontitis as keywords. Articles in English language issued in the last 10 years were screened, in which green tea was imperatively used as a therapeutic approach and included randomized controlled trials and systematic reviews. Pre-clinical trials, clinical case reports, series of case reports, epidemiological studies, editorials, expert opinions, pilot study, literature review, researches with inconclusive results, and in-vitro studies were excluded. The search resulted in 450 articles in which, after applying inclusion and exclusion criteria, 10 articles were screened, being 10 articles, 8 randomized controlled trials, and 2 systemic reviews and meta-analysis. 6 articles assessed the effectiveness of green tea as mouthwash and presenting a reduction either in inflammation and the accumulation of bacterial plaque, 3 studies associated green tea to scaling and root planing, indicating a decrease in the depth, bleeding when drilling, and reduction in the inflammatory process, 1 study assessed the systemic intake, indicating a reduction in drilling depth and bleeding. Thus, we can conclude that green tea is efficient as adjuvant therapy to the treatment and control of periodontitis. When associated with scaling and root planing, and used as a mouthwash, the results were more satisfactory when it was used as a mouthwash than when it was ingested. Keywords: Tea. Camelliasinensis. Periodontal Diseases. Periodontology.

Made available in DSpace on 2022-05-01T09:47:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background and objective: The interaction between antineoplastic drugs used for treating cancer and non-affected tissues remains poorly assessed and may be critical for maintaining the quality of life for patients during and after treatment. This pre-clinical study evaluated the effects of cisplatin (CIS) and 5-fluorouracil (5-FU) on the peri-implant repair process around osseointegrated titanium implants installed in the tibiae of rats. Material and methods: Were used 90 male rats, randomly divided into three groups (n = 30): physiological saline solution (PSS), CIS, and 5-FU. Titanium implants (4.0 × 2.2 mm) were inserted in both tibiae of all animals at day 0. The animals received either PSS, CIS, or 5-FU at 35 and 37 days. Euthanasia was performed at 50, 65, and 95 days after surgery. Histometric (bone/implant contact [BIC]) and bone area fraction occupancy (% BAFO), histological, and immunohistochemical (for bone morphogenetic protein 2/4 [BMP2/4], Runt-related transcription factor 2 [RUNX2], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analyses were performed. Data were statistically analyzed. Results: Groups CIS and 5-FU presented lower BIC and lower BAFO as compared with PSS in all time points. The imbalance in bone turnover was observed by the lower number of BMP2/4-, RUNX2-, and OCN-positive cells/mm2 and the higher number of TRAP-positive cells/mm in groups CIS and 5-FU as compared with PSS in all time points. Persistent and exacerbated inflammation was observed in groups CIS and 5-FU. Conclusions: Both antineoplastic agents interfered negatively in the bone turnover around osseointegrated titanium implants. Clinical relevance: Closer and more careful follow-up of patients with osseointegrated implants that will undergo chemotherapy with either CIS or 5-FU shall be performed. Department of Diagnosis and Surgery–Periodontics Division São Paulo State University (Unesp), St. José Bonifácio 1193 - Vila Mendonça, SP Nucleus of Study and Research in Periodontics and Implantology (NEPPI) School of Dentistry São Paulo State University (Unesp), SP Department of Basic Science School of Dentistry São Paulo State University (Unesp), SP Department of Diagnosis and Surgery–Periodontics Division São Paulo State University (Unesp), St. José Bonifácio 1193 - Vila Mendonça, SP Nucleus of Study and Research in Periodontics and Implantology (NEPPI) School of Dentistry São Paulo State University (Unesp), SP Department of Basic Science School of Dentistry São Paulo State University (Unesp), SP FAPESP: 2014/11427-8 FAPESP: 2017/11805-0

Made available in DSpace on 2022-04-29T08:35:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Purpose: The determination on how antineoplastic agents interfere on the progression of periodontitis is critical for improvement and even development of novel therapeutic approaches for periodontal management. This study evaluated the influence of chemotherapy with 5-fluorouracil (5-FU) or cisplatin (CIS) on healthy periodontal tissues and on the progression of experimental periodontitis (EP). Methods: One hundred forty-four male rats were divided into six groups (n = 24). Each group was treated with physiological saline solution (PSS) 0.9%, 5-FU, or CIS. Experimental periodontitis (EP) was induced by ligature placement. Animals were euthanized at 7, 15, and 30 days after treatment. Data were statistically analyzed (p ≤ 0.05). Results: The groups with EP and treated with 5-FU or CIS showed lower percentage of bone volume in the furcation region and higher percentage of alveolar bone loss, higher number of TRAP-positive cells, and lower number of PCNA-positive cells when compared group with EP and treated with PSS (p ≤ 0.05). Groups with EP and treated with 5-FU or CIS showed high immunolabelling pattern of RANKL, TNF-α, and IL-1β, moderate of BAX, and low of HIF-1α. Histological analysis showed severe tissue breakdown in the groups with EP and treated with 5-FU or CIS. Conclusions: Chemotherapy with antineoplastic agents 5-FU and CIS increased the intensity and duration of the inflammation and compromised tissue repair by reduction in cellular and vascular turnover. The more severe periodontal breakdown was caused by 5-FU. Department of Diagnosis and Surgery Division of Periodontics School of Dentistry of Araçatuba São Paulo State University (UNESP), St. José Bonifácio 1193 - Vila Mendonça, SP Department of Basic Science Histology Division School of Dentistry of Araçatuba São Paulo State University (UNESP), SP Department of Diagnosis and Surgery Division of Periodontics School of Dentistry of Araçatuba São Paulo State University (UNESP), St. José Bonifácio 1193 - Vila Mendonça, SP Department of Basic Science Histology Division School of Dentistry of Araçatuba São Paulo State University (UNESP), SP FAPESP: #2014/11427-8

To assess the interaction between chemotherapy and normal tissues is critical to assure quality of life during and after the treatment of cancer. This study evaluated the influence of cisplatin (CIS) and 5-fluorouracil (5-FU) over the peri-implant tissues around osseointegrated titanium implants in animals previously exposed to nicotine. Materials and methods One hundred twenty male rats were divided into two groups, receiving via subcutaneous injection, either physiological saline solution (PSS) (n = 30) or nicotine hemissulfate (NIC) (n = 90) for 30 days prior to implants' placement. One titanium implant (4.0 × 2.2 mm) was installed in each tibia of all animals. PSS and NIC were continued for 30 days after surgery. Five days after cessation, rats were subdivided into three subgroups in accordance with systemic treatments with either PSS, CIS, or 5-FU. Euthanasia was performed at 50, 65, and 95 days post-surgery. Histometric, histopathological, and immunohistochemical analyses were performed.NIC-CIS and NIC-5FU presented lower BIC (50, 65, and 95 days) and bone area fraction occupancy (BAFO) (65 and 95 days) than group NIC. Intense inflammatory infiltration, severe tissue breakdown, reduced expression of bone formation biomarkers, and upregulation of TRAP were observed in NIC-CIS and NIC-5FU when compared with group NIC. TRAP expression was significantly higher in NIC-5FU as compared with NIC-CIS at 50 and 95 days. Groups NIC, NIC-CIS, and NIC-5FU presented statistically significant negative impact in all outcome parameters than group PSS.CIS and 5-FU severely disrupted the peri-implant tissues around osseointegrated implants in animals previously exposed to nicotine.Assessing the interaction between chemotherapy and normal tissues is critical to assure quality of life during and after the cancer treatment.

Dental agenesis consists of an anomaly characterized by the numerical reduction of dental elements and, according to the number of missing teeth, it may receive other names, such as hypodontia, oligodontia, anodontia or congenital absence. Several treatments may be recommended depending on the patient's needs and conditions, such as surgery for the installation of implants and fixed prostheses, and early diagnosis is one of the key parts for proper treatment. The rehabilitation of agenesis of the upper lateral incisor is a great challenge for the professional since several factors can influence the aesthetic result and, in addition, the absence of the dental element can bring unbalance of the occlusion and phonetic problems. It is essential to carry out an adequate planning and a treatment plan with multidisciplinary action so that the final result is effective and meets the expectations of each patient. The present study aimed to report a clinical case of dental agenesis of the upper lateral incisor, where the treatment of choice was the installation of an implant-supported prosthesis and filling of the buccal bone plate with biomaterial. La agenesia dental consiste en una anomalía caracterizada por la reducción numérica de elementos dentarios y, según el número de dientes faltantes, puede recibir otros nombres, como hipodoncia, oligodoncia, anodoncia o ausencia congénita. Se pueden recomendar varios tratamientos en función de las necesidades y condiciones del paciente, como la cirugía para la instalación de implantes y prótesis fijas, y el diagnóstico precoz es una de las piezas clave para un tratamiento adecuado. La rehabilitación de la agenesia del incisivo lateral superior es un gran reto para el profesional ya que varios factores pueden influir en el resultado estético y, además, la ausencia del elemento dental puede traer desequilibrio de la oclusión y problemas fonéticos. Es fundamental llevar a cabo una adecuada planificación y un plan de tratamiento con acción multidisciplinar para que el resultado final sea efectivo y cumpla con las expectativas de cada paciente. El presente estudio tuvo como objetivo reportar un caso clínico de agenesia dental del incisivo lateral superior, donde el tratamiento de elección fue la instalación de una prótesis implantosoportada y el relleno de la placa ósea vestibular con biomaterial. A agenesia dentária consiste em uma anomalia caracterizada pela redução numérica dos elementos dentários e de acordo com o número de dentes faltantes pode receber outras denominações, como hipodontia, oligodontia, anodontia ou ausência congênita. Diversos tratamentos podem ser preconizados dependendo da necessidade e condições do paciente, como cirurgia para instalação de implantes e próteses fixas, sendo que o diagnóstico precoce é uma das peças-chaves para o tratamento adequado. A reabilitação de agenesia de incisivo lateral superior é um grande desafio para o profissional visto que diversos fatores podem influenciar no resultado estético e além disso, a ausência do elemento dental pode trazer desequilíbrio da oclusão e problemas fonéticos. É fundamental a realização de um planejamento adequado e um plano de tratamento com ação multidisciplinar para que o resultado final seja efetivo e supra as expectativas de cada paciente. O presente trabalho objetivou relatar um caso clínico de agenesia dental de incisivo lateral superior, onde o tratamento de escolha foi a instalação de uma prótese implantossuportada e preenchimento da tábua óssea vestibular com biomaterial.

Made available in DSpace on 2019-10-06T17:07:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-06-01 Aim: This study evaluated the effects of topical green tea extract solution (GTE) as adjuvant therapy to mechanical debridement for the treatment of experimental periodontitis (EP). Material and methods: We used 120 male rats (Rattus norvegicus albinus – Wistar), divided into the following four groups: NEP (sham) (n = 30): no experimental periodontitis (NEP), only simulation of EP by installation and removal of a ligature; EP (n = 30): EP induction by ligature; SRP (n = 30): EP, scaling and root planing (SRP), and irrigation with physiological saline solution; SRP/GT (n = 30): EP, SRP, and irrigation with GTE. Histologic analysis and immunohistochemistry were performed for detection of interleukin (IL)1ß, tumor necrosis factor-alpha (TNF-α), IL-10, and anti-tartrate resistant acid phosphatase (TRAP) in the furcation area. The percentage of bone in the furcation (PBF) was considered the primary variable and evaluated at 14, 22, and 37 days. The data from the histological analysis and the IL- 1B, TNF- A, and IL-10 were submitted to a Kruskal-Wallis variance test and Dunn's posttest (p ≤ 0.05). The histometric data and TRAP were submitted to analysis of variance (ANOVA) and Tukey's posttest (p ≤ 0.05). Results: The SRP/GT group showed lower inflammatory process, lower immunolabeling pattern of IL-1ß and TNF-α, and greater immunolabeling pattern of IL-10 compared with the EP and SRP groups at 22 days. Fewer TRAP-positive multinucleated osteoclasts were observed in all periods in the SRP/GT group (5.22 ± 0.65; 7.33 ± 0.80; 8.55 ± 1.15) compared with the SRP group (30.67 ± 8.55; 13.22 ± 0.77; 13.87 ± 0.77). Higher PBF was observed in all periods in the SRP/GT group (74.65 ± 7.14; 76.61 ± 5.36; 79.24 ± 3.75) compared with the SRP group (61.60 ± 9.48; 54.84 ± 9.06; 53.25 ± 9.66). Conclusion: GTE adjuvant to SRP reduced inflammation, osteoclastic activity, and alveolar bone loss in EP. São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic – Periodontics Division São Paulo State University (UNESP) School of Dentistry Department of Basic Science – Histology Division São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic – Periodontics Division São Paulo State University (UNESP) School of Dentistry Department of Basic Science – Histology Division

Made available in DSpace on 2019-10-06T16:53:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) in the alveolar repair of rats with major risk factors for bisphosphonate-related osteonecrosis of the jaws (BRONJ). Senile rats received 0.45 ml of vehicle (VEH and VEH/aPDT) or 0.45 ml of zoledronate (ZOL and ZOL/aPDT) every three days for seven weeks. After three weeks of treatment, the first lower left molar was extracted. VEH/aPDT and ZOL/aPDT were submitted to aPDT on the extraction site at 0, 2 and 4 days postoperatively. Euthanasia was performed 28 days postoperatively and the extraction site was evaluated by clinical, histological, histometric, histochemical and immunohistochemical analysis. ZOL showed tissue repair impairment; lower percentage of newly formed bone tissue (NFBT); higher percentage of non-vital bone tissue (NVBT); fewer mature collagen fibers and increased immunolabeling for tumor necrosis factor (TNFα), interleukin (IL)-1β and IL-6. ZOL/aPDT showed clinical and histological characteristics of the extraction site, percentage of NFBT and percentage of mature collagen fiber similar to VEH. Percentage of NVBT and immunolabeling for inflammatory cytokines in ZOL/aPDT was lower than in ZOL. Immunolabeling for tartarato-resistant acid phosphatase (TRAP) was lower in ZOL and ZOL/aPDT. aPDT in the dental extraction site improves tissue repair process and prevents the occurrence of BRONJ-like lesions after tooth extraction. São Paulo State University (UNESP) School of Dentistry Department of Basic Sciences, Rua José Bonifácio, 1193 São Paulo State University (UNESP) Institute of Biosciences, Rua Prof. Dr. Antônio Celso Wagner Zanin, 250 São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic, Rua José Bonifácio, 1193 São Paulo State University (UNESP) School of Dentistry Department of Pediatric Dentistry and Public Health, Rua José Bonifácio, 1193 São Paulo University (USP) School of Dentistry Department of Morphology Physiology and Basic Pathology, Avenida do Café s/n Group for the Research and Study of Laser in Dentistry São Paulo State University (UNESP) School of Dentistry, Rua José Bonifácio, 1193 São Paulo State University (UNESP) School of Dentistry Department of Basic Sciences, Rua José Bonifácio, 1193 São Paulo State University (UNESP) Institute of Biosciences, Rua Prof. Dr. Antônio Celso Wagner Zanin, 250 São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic, Rua José Bonifácio, 1193 São Paulo State University (UNESP) School of Dentistry Department of Pediatric Dentistry and Public Health, Rua José Bonifácio, 1193 Group for the Research and Study of Laser in Dentistry São Paulo State University (UNESP) School of Dentistry, Rua José Bonifácio, 1193 FAPESP: 2012/03067-6 FAPESP: 2013/25367-4 FAPESP: 2013/26779-4 FAPESP: 2014/02199-1 FAPESP: 2015/22395-2 CNPq: 28040-2013 CNPq: 30699-2014

Objetivo: O objetivo deste trabalho é descrever uma revisão narrativa de literatura sobre os efeitos dos agentes quimioterápicos no periodonto e salientar sobre a importância clínica dos cirurgiões dentistas durante a terapia multidisciplinar em pacientes oncológicos. Metodologia: Uma pesquisa bibliográfica exploratória foi realizada nas bases de dados eletrônicas Pubmed/Medline, Embase, Scopus e Scielo, utilizando os termos de busca “chemotherapy and periodontitis”, “chemotherapy and gingivitis” e “chemotherapy and periodontal disease”, sendo considerado os artigos publicados até junho de 2022. Resultados e discussão: Os quimioterápicos não atuam sobre células específicas, eles afetam células de rápida proliferação celular e causam citotoxicidade várias células do tecido conjuntivo. Desta forma, os quimioterápicos causam destruição do epitélio juncional e exacerbam o processo inflamatório do tecido conjuntivo gengival. Quando a periodontite que ocorre pela interação placa bacteriana e o hospedeiro se instala, os quimioterápicos agravam sua progressão. Além disso, quimioterápicos podem afetar os tecidos periodontais de maneira indireta por causar xerostomia e aumentar a quantidade de bactérias da boca. As doenças periodontais são fatores de risco para doenças sistêmicas como: doenças cardiovasculares, doenças do trato respiratório, diabetes, doença de Alzheimer e resultados adversos da gravidez e cabe ao cirurgião dentista da equipe multidisciplinar acompanhar e identificar possíveis riscos no tratamento bucal de pacientes oncológicos. Conclusão: Dentro dos limites da presente revisão de literatura, pode-se concluir que os tecidos periodontais são agredidos pela utilização dos quimioterápicos e medidas de prevenção ou formas de tratamento devem ser levadas em consideração pelo cirurgião dentista da equipe multidisciplinar oncológica.

The Peripheral Ossifying Fibroma is a benign tumor that develops from a hyperplastic tissue reaction, usually related to traumatic stimulus that are responsible for triggering inflammatory reactions of the connective tissue. Histologically, it is a nodular mass characterized by a dense connective tissue, surrounded by stratified squamous epithelium. Surgical removal in these cases is indicated, and for reconstruction of soft tissue in the region, some periodontal surgical techniques are recommended, such as free gingival grafting. Thus, the present study aims to report a clinical case submitted to the free gingival graft technique for tissue reconstruction after the surgical removal of a fibroma. A total excision of the lesion was performed, later sent to a histopathological report where it was diagnosed as Peripheral Ossifying Fibroma, after the removal of the lesion the region was left with the periosteum exposed and then the free gingival graft was performed to cover the region and promote keratinized gum augmentation. This technique proved to be efficient for reconstruction of soft tissue in the region after surgical removal of the Peripheral Ossifying Fibroma, returning aesthetics, function and periodontal health. El fibroma osificante periférico es un tumor benigno que se desarrolla a partir de una reacción tisular hiperplásica, generalmente relacionada con estímulos traumáticos que son responsables de desencadenar reacciones inflamatorias del tejido conectivo. Histológicamente, es una masa nodular caracterizada por un tejido conectivo denso, rodeado de epitelio escamoso estratificado. La extirpación quirúrgica en estos casos está indicada, y para la reconstrucción de los tejidos blandos de la región, están indicadas algunas técnicas quirúrgicas periodontales, como el injerto gingival libre. Así, el presente estudio tiene como objetivo informar de un caso clínico sometido a la técnica del injerto gingival libre para la reconstrucción de tejidos tras la extirpación quirúrgica de un fibroma osificante periférico. Se realizó una escisión total de la lesión, posteriormente se envió a informe histopatológico donde se diagnosticó como fibroma, tras la extirpación de la lesión se dejó la región con el periostio expuesto y posteriormente se realizó el injerto gingival libre para cubrir la región y promover el agrandamiento gingival queratinizado. Esta técnica demostró ser eficaz para la reconstrucción de los tejidos blandos de la región tras la extirpación quirúrgica del fibroma, com la devolución de la estética, la función y la salud periodontal. O Fibroma Ossificante Periférico é tumor benigno que se desenvolve a partir de uma reação hiperplásica tecidual, habitualmente relacionada a estímulos traumáticos que são responsáveis por desencadear reações inflamatórias do tecido conjuntivo. Histologicamente, é uma massa nodular caracterizada por um tecido conjuntivo denso, circundado por epitélio escamoso estratificado. A remoção cirúrgica nestes casos é indicada, e para reconstrução de tecido mole na região, algumas técnicas cirúrgicas periodontais são indicadas, como o enxerto gengival livre. Assim, o presente estudo tem como objetivo relatar um caso clínico submetido à técnica de enxerto gengival livre para reconstrução tecidual após a remoção cirúrgica de um fibroma. Foi realizada a excisão total da lesão, posteriormente enviado para laudo histopatológico onde foi diagnosticada como fibroma, após a remoção da lesão a região ficou com o periósteo exposto e então foi realizado o enxerto gengival livre para recobrir a região e promover aumento de gengiva queratinizada. Esta técnica se mostrou eficiente para reconstrução do tecido mole na região após a remoção cirúrgica do fibroma. Devolvendo estética, função e saúde periodontal.

Made available in DSpace on 2022-05-01T11:39:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Universidade Estadual Paulista Background: Strontium Ranelate (SR) presents overlapping osteoanabolic and anti-resorptive activity. However, the effects of SR on the progression of periodontitis through the alveolar bone and its potential applicability as adjunctive therapy to scaling and root planning remain poorly accessed. The aim of this study was to evaluate the effects of systemic (SR) both on the progression of experimental periodontitis (EP) and as adjunctive therapy to SRP. Material and Methods: Eighty male rats were divided into four groups (n=20): EP-PSS: EP induction and systemic administration of physiological saline solution (PSS); EP-SR: EP induction and systemic administration of SR; EPSRP/ PSS: EP induction, SRP and systemic administration of PSS; EP-SRP/SR: EP induction, SRP and systemic administration of SR. Seven days after ligature placement, SRP was performed in EP-SRP/PSS and EP-SRP/SR, as well as the systemic administration of either PSS or SR were initiated and continued until euthanasia in all groups. Animals were euthanized at 7 and 30 days after the beginning of the systemic treatments. Histological, histometric (percentage of bone in the furcation [PBF]) and immunohistochemical (tartrate-resistant acid phosphatase [TRAP], Osteocalcin [OCN] and leukocyte common antigen [CD 45]) analyses were performed. Data were statistically analyzed. Results: EP-SRP/PSS showed a significantly more organized pattern of the connective tissue and alveolar bone structure than EP-SRP/SR. EP-SR showed significantly higher PBF than EP-PSS, however, EP-SRP/PSS showed no difference with EP-SRP/SR at 30 days. Conclusions: SR reduced the alveolar bone loss in non-treated animals and presented no standout benefits over the conventional forms of treating EP. Department of Diagnosis and Surgery-Division of Periodontics Sao Paulo State University (UNESP) School of Dentistry Nucleus of Study and Research in Periodontics and Implantology (NEPPI) São Paulo State University (Unesp) School of Dentistry Department of periodontics University of Western Sao Paulo (UNOESTE) Presidente Prudente Department of Basic Sciences School of Dentistry Sao Paulo State University (UNESP) Department of Diagnosis and Surgery-Division of Periodontics Sao Paulo State University (UNESP) School of Dentistry Nucleus of Study and Research in Periodontics and Implantology (NEPPI) São Paulo State University (Unesp) School of Dentistry Department of Basic Sciences School of Dentistry Sao Paulo State University (UNESP)

Purpose The aim of this study was to evaluate the effects of locally delivered 1% alendronate (ALN) gel used as an adjunct to non-invasive periodontal therapy. Methods Ligature-induced periodontitis was performed in 96 rats. The ligature was tied in the cervical area of the mandibular left first molar. The animals were randomly divided into 4 groups: 1) NT, no treatment; 2) SRP, scaling and root planning; 3) SRP/PLA, SRP followed by filling the periodontal pocket with placebo gel (PLA); and 4) SRP/ALN, SRP followed by filling the periodontal pockets with 1% ALN gel. Histomorphometric (percentage of bone in the furcation region [PBF]) and immunohistochemical (receptor activator of nuclear factor-κB ligand, osteoprotegerin, and tartrate-resistant acid phosphatase) analyses were performed. Data were statistically analyzed, with the threshold of statistical significance set at P≤0.05. Results The SRP, SRP/PLA, and SRP/ALN groups presented a higher PBF than the NT group (P≤0.01) at 7, 15, and 30 days. The SRP/ALN group presented a higher PBF than the SRP/PLA group in all experimental periods, as well as a higher PBF than the SRP group at 15 and 30 days. No differences were observed in the immunohistochemical analyses (P>0.05 for all). Conclusions Locally delivered 1% ALN gel used as an adjunct to SRP enhanced bone regeneration in the furcation region in a rat model of experimental periodontitis., Graphical Abstract

Made available in DSpace on 2021-06-25T11:09:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Background: This study is designed to evaluate the potential of different formulations of hyaluronic acid (HA) to improve new bone formation in critical-size calvaria defect (CSD) when combined with a deproteinized bovine graft (DBG) material. Methods: Thirty male rats were used. A 5-mm-diameter CSD was created and three experimental groups (n = 10) were randomly assigned based on the treatments performed. Group DBG: CSD filled with a DBG; group DBG/LV: CSD filled by the combination of DBG and HA in a low-viscosity crosslinking agent; group DBG/HV: CSD filled by the combination of DBG and HA in a high-viscosity crosslinking agent. Animals were euthanized 30 days postoperatively. Histological, histometric (percentage of newly formed bone [PNFB], percentage of remaining graft particles, histochemical, and immunohistochemical (bone morphogenetic protein 2/4 [BMP2/4], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analyses were performed. Results: The highest PNFB was observed in DBG/HV when compared with the other groups (P ≤0.05). DBG/LV and DBG/HV presented almost no inflammatory cells. In contrast, inflammation was observed in group DBG. Extensive resorption of graft particles was observed in group DBG, which was not present in DBG/LV and DBG/HV as confirmed by the larger size of the particles (P ≤0.05). BMP2/4 and OCN immunolabeling were higher in DBG/HV when compared with group DBG (P ≤0.05). Increased number of TRAP-positive cells was observed in DBG/LV and DBG/HV (P ≤0.05). Lower percentage of mature collagen fibers was observed in DBG/HV (P ≤0.05). Conclusion: The combination of HA in a high-viscosity crosslinking agent with DBG improves the bone repair process and increases the amount of newly formed bone towards CSDs in rat calvaria. Department of Diagnosis and Surgery‒Periodontics Division School of Dentistry São Paulo State University (UNESP) Department of Basic Science School of Dentistry São Paulo State University (UNESP) Private practice. Director of the Pro-clinic Nucleus of Orofacial Harmonization Department of Diagnosis and Surgery‒Periodontics Division School of Dentistry São Paulo State University (UNESP) Department of Basic Science School of Dentistry São Paulo State University (UNESP)

Made available in DSpace on 2022-04-28T19:46:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Background. The potent anti-inflammatory and immunosuppressive properties of glucocorticoids (GCs) might influence the progression of some disorders, such as periodontitis. Hence, this study aimed to investigate the influence of dexamethasone (DEX) on the alveolar bone loss (ABL) of healthy and periodontally compromised molars in rats. Methods. Thirty male rats were randomly assigned to two groups: physiological saline solution (PSS) and DEX. The animals received subcutaneous injections of either 0.5 mL of PSS) (group PSS) or 2 mg/kg of DEX (group DEX) from one day before experimental periodontitis (EP) induction until euthanasia. EP was induced through ligature placement around the mandibular lower first molars at day 0. Contralateral molars remained unligated. Ten animals per period were euthanized on days 3, 7, and 14. Morphometric analysis was performed to access the ABL. Data were statistically analyzed with ANOVA followed by post hoc Tukey tests (P ≤ 0.05). Results. Higher ABL was observed in both groups on days 7 and 14 than on day 3 (P ≤ 0.05). Concerning periodontitis, higher ABL was observed in group DEX on days 3, 7, and 14 days than group PSS at the same time intervals (P ≤ 0.05). Also, even in the contralateral unligated molars, group DEX exhibited higher ABL on days 3, 7, and 14 days than group PSS at the same time intervals (P ≤ 0.05). Conclusions. Collectively, it can be concluded that DEX aggravates EP and induces spontaneous ABL in the healthy periodontium. Department of Diagnosis and Surgery São Paulo State University (UNESP) School of Dentistry Department of Diagnosis and Surgery São Paulo State University (UNESP) School of Dentistry

Made available in DSpace on 2021-06-25T10:22:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Background: Platelet-rich fibrin (PRF) has been referred to as a second-generation platelet concentrate, associated with improvements on the healing of palatal wounds followed by FGG harvesting. The aim of this systematic review and meta-analysis was to assess the complete wound epithelialization and postoperative pain when PRF was used in palatal wounds following free gingival graft (FGG) harvesting. Material and Methods: PubMed (Medline), EMBASE and Scopus were searched by two independent individuals up to and including March 2020 in order to identify controlled and randomized controlled clinical trials on the use of PRF at palatal donor sites of FGG. The outcomes assessed were epithelialization and postoperative pain. The risk of bias of the included studies was evaluated using Cochrane Collaboration's domain-based two-part tool. Random effects meta-analyses were conducted with 95% confidence intervals. Results: The search strategy identified 555 potentially eligible articles, of which 6 randomized controlled clinical trials were included. In the qualitative analysis, most studies (83.3%) reported lower postoperative pain in treatment groups, while all studies accessing epithelialization demonstrated earlier complete wound closure in groups treated with PRF. The discomfort and complete re-epithelialization were more favorable in groups PRF when compared to control groups (P

BackgroundThis study evaluated the effects of the chronic consumption of different concentrations of alcohol on the experimental periodontitis (EP).Methods160 rats were divided into 4 groups: (EP-NT) rats with EP and no alcohol exposure; (EP-A14) rats with EP exposed to 14% alcohol; (EP-A25) rats with EP exposed to 25% alcohol; (EP-A36) rats with EP exposed to 36% alcohol. The animals from the EP-A14, EP-A25 and EP-A36 groups were subjected to different concentrations of alcohol 30 days before EP induction. The histological characteristics, percentage of bone in the furcation (PBF) and bone metabolism in the furcation region were evaluated. The PBF and tartrate-resistant acid phosphatase (TRAP) data were subjected to statistical analysis.ResultsThe EP-A14, EP-A25 and EP-A36 groups had lower PBFs compared with the EP-NT group. A more severe inflammatory process and a greater number of TRAP+ cells were also observed. In the EP-A14, EP-A25 and EP-A36 groups, the inflammatory process became more severe as the ingested alcoholic concentration increased. An increase in RANKL immunostaining and a significantly higher number of TRAP+ cells were also observed.ConclusionWe conclude that chronic alcohol consumption increases the severity of experimental periodontitis in a dose-dependent manner by increasing the magnitude of local inflammatory responses and stimulating alveolar bone resorption.

Made available in DSpace on 2018-12-11T17:37:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-07-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The aim of this study was to evaluate the effects of photobiomodulation (PBM) with multiple sessions of low-level laser on the alveolar repair process of rats with major risk factors for medication-related osteonecrosis of the jaws (MRONJ). Senile rats received 0.45 mL of vehicle (VEH and VEH-PBM) or 0.45 mL of 100 μg/kg zoledronate (ZOL and ZOL-PBM) administrated intraperitoneally every two days during seven weeks. After three weeks of initiation of drug treatment the first lower left molar was extracted. No local treatment was performed in VEH and ZOL. VEH-PBM and ZOL-PBM were submitted to laser irradiation (660 ± 10 nm; 0.035 W; 2.1 J; 60 s) on the extraction site at 0, 2 and 4 days postoperatively. Euthanasia was performed 28 days after tooth extraction. Histological sections of the hemimandible were submitted to histopathological and histomorphometric analysis, as well as to histochemistry for collagen fiber maturation and immunohistochemistry for pro-inflammatory cytokines. In ZOL, general impairment of tissue repair, areas with osteonecrosis, lower newly formed bone tissue (NFBT), smaller amount of mature collagen fibers and increased immunoreactivity for TNFα IL-1β and IL-6 were observed when compared to VEH and VEH-PBM. ZOL-PBM showed significant improvement in some parameters compared to ZOL, such as positive repair tissue, higher NFBT, greater amount of mature collagen fibers, besides TNFα and IL-1β immunoreactivity decrease. Zoledronate treatment severely compromised the tissue repair process of the tooth extraction site in rats with major risk factors for MRONJ. Based on parameters employed in the present study, PBM in multiple sessions can improve the alveolar repair process, constituting a promising preventive therapy to avoid the onset of post-extraction MRONJ. Group for the Research and Study of Laser in Dentistry School of Dentistry of Araçatuba São Paulo State University (UNESP) Department of Surgery and Integrated Clinic School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, CEP Department of Basic Science School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, CEP Institute of Biosciences São Paulo State University (UNESP), Rua Prof. Dr. Antônio Celso Wagner Zanin, 250, CEP Department of Morphology Physiology and Basic Pathology School of Dentistry São Paulo University (USP), Avenida do Café s/n, CEP Department of Restorative Dentistry School of Dentistry São Paulo StateUniversity (UNESP), Rua José Bonifácio, 1193, CEP Group for the Research and Study of Laser in Dentistry School of Dentistry of Araçatuba São Paulo State University (UNESP) Department of Surgery and Integrated Clinic School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, CEP Department of Basic Science School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, CEP Institute of Biosciences São Paulo State University (UNESP), Rua Prof. Dr. Antônio Celso Wagner Zanin, 250, CEP Department of Restorative Dentistry School of Dentistry São Paulo StateUniversity (UNESP), Rua José Bonifácio, 1193, CEP FAPESP: #2012/03067-6 FAPESP: #2013/26779-4 FAPESP: #2014/02199-1 FAPESP: #2014/14118-6 CNPq: #28040-2013 CNPq: #30699-2014

Despite clinical trials and reviews attempt to assess a possible relationship between dementia and periodontal disease, no meta-analysis has been performed and this issue remains undetermined. The aim of this study is to conduct a systematic review and meta-analysis to assess severity of periodontitis in subjects with dementia.The search was conducted in Pubmed, Embase/MEDLINE. Two independent reviewers extracted data and assessed the risk bias (Newcastle-Ottawa scale). Meta-analyses were performed using the means of probing depth (PD) and clinical attachment loss (CAL) in patients with or without dementia. The mean difference were analyzed (P ≤ 0.05).Fourteen studies were included in the systematic review. In the qualitative analysis, most studies reported higher prevalence of periodontal disease in dementia patients. The studies had low risk of bias and two meta-analyses were performed for each parameter, including or not a cross-sectional study. The meta-analyses including the cross-sectional study demonstrated significant association between dementia and periodontal disease (mean difference: PD = 1.41; CAL = 1.40, P 0.05), however, it wasn't confirmed when the cross-sectional study was removed (1.25 mm, P 0.22) and CAL (1.20 mm, P 0.22).Although the qualitative analysis have suggested worse periodontal conditions in dementia patients, due to different study types and the high heterogeneity among them, the meta-analysis does not support the association between dementia and severity of periodontal disease.

AIM The purpose of this study in animals was to evaluate the peri-implant bone repair against systemic administration of the antineoplastic agent. MATERIAL AND METHODS We used 84 male rats (Rattus norvegicus, albinus, Wistar), divided into two groups: cisplatin (CIS) and saline solution (SS). The titanium implants were inserted into the right tibia at day 0 in all animals from both groups. Group SS received SS intraperitoneally at 15 and 17 days postoperatively. Group CIS received 5 and 2.5 mg/kg of CIS intraperitoneally at 15 and 17 days postoperatively, respectively. Euthanasia was performed at 22, 30 and 60 days postoperatively. Twenty-four undecalcified specimens were prepared for histometric analysis of bone/implant contact (BIC). Sixty specimens were selected to bone area (BA) measurement, histological analysis and immunohistochemical analysis of RUNX-2, osteocalcin (OCN) and tartrate-resistant acid phosphatase (TRAP). BIC and BA were considered to be the primary outcome parameters. RESULTS Group CIS showed lower BIC (11.87 ± 0.97 mm; 19.19 ± 0.8 mm; 17.69 ± 1.05 mm; p ≤ .05) and BA (3.68 ± 1.29 mm2 ; 3.05 ± 0.88 mm2 ; 3.23 ± 0.67 mm2 ; p ≤ .05), as well as decreased number of RUNX-2 (102.8 ± 27.35 cells/mm2 ; 100.04 ± 8.61 cells/mm2 ; 118.82 ± 21.38 cells/mm2 ; p ≤ .05)- and OCN-positive cells (120 ± 24.5 cells/mm2 ; 102 ± 27.73 cells/mm2 ; 100 ± 14.23 cells/mm2 ; p ≤ .05) at 22, 30 and 60 days, respectively. The animals in group CIS also showed increased number of TRAP-positive cells (86.8 ± 6.37 cells/mm2 ; 71.5 ± 4.72 cells/mm2 ; 92.8 ± 9.52 cells/mm2 ; p ≤ .05) and a persistent and exacerbated inflammatory response in all experimental periods. CONCLUSION Within the limits of this study, it was concluded that the chemotherapeutic CIS negatively affects the bone repair at peri-implant areas, jeopardizing the osseointegration of titanium implants.

Made available in DSpace on 2018-12-11T17:05:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective To histomorphometrically analyze the effect of low-level laser therapy (LLLT) on bone formation process in surgically created critical-size defects (CSDs) treated with bovine bone graft (BBG) and its influence over particles' resorption of BBG. Methods A 10-mm diameter CSD was surgically created in the calvaria of 64 male rats, which were distributed into 4 experimental groups: the C group (control), only blood clot; the LLLT group, LLLT (GaAlAs, 660 nm) and blood clot; the BBG group, CSD filled with BBG; the BBG/LLLT group, LLLT and CSD filled with BBG. Animals were euthanized at either 30 or 60 days post-operation. A histological analysis was performed. Additionally, the percentage of newly formed bone area (NFBA) and remaining particles areas (RPA) of BBG were histometrically evaluated and data statistically analyzed. Results The LLLT (5.82 ± 2.05; 7.34 ± 1.01) group presented significantly greater NFBA when compared to the C group (1.61 ± 0.30; 5.59 ± 0.94) at 30 and 60 days post-operation (p

Made available in DSpace on 2019-10-06T17:00:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study evaluated the effects of local application of autologous platelet-rich plasma (PRP) on the tooth extraction site of rats presenting the main risk factors for medication-related osteonecrosis of the jaw (MRONJ). For seven weeks, senile rats were submitted to systemic treatment with vehicle (VEH and VEH-PRP) or 100 μg/Kg of zoledronate (ZOL and ZOL-PRP) every three days. After three weeks, the first lower molar was extracted. VEH-PRP and ZOL-PRP received PRP at the tooth extraction site. Euthanasia was performed at 28 days postoperatively. Clinical, histopathological, histometric and immunohistochemical analyses were carried out in histological sections from the tooth extraction site. ZOL showed lower percentage of newly formed bone tissue (NFBT), higher percentage of non-vital bone tissue (NVBT), as well as higher immunolabeling for TNFα and IL-1β. In addition, ZOL presented lower immunolabeling for PCNA, VEGF, BMP2/4, OCN and TRAP. VEH and ZOL-PRP showed improvement in the tooth extraction site wound healing and comparable percentage of NFBT, VEGF, BMP2/4 and OCN. Local application of autologous PRP proved a viable preventive therapy, which is safe and effective to restore tissue repair capacity of the tooth extraction site and prevent the occurrence of MRONJ following tooth extraction. São Paulo State University (UNESP) School of Dentistry Department of Basic Sciences Rua José Bonifácio 1193 CEP São Paulo State University (UNESP) Institute of Biosciences Rua Prof. Dr. Antônio Celso Wagner Zanin 250 CEP São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic Rua José Bonifácio 1193 CEP São Paulo State University (UNESP) School of Dentistry Department of Restorative Dentistry Rua José Bonifácio 1193 CEP São Paulo University (USP) School of Dentistry Department of Morphology Physiology and Basic Pathology Avenida do Café s/n CEP São Paulo State University (UNESP) School of Dentistry Department of Basic Sciences Rua José Bonifácio 1193 CEP São Paulo State University (UNESP) Institute of Biosciences Rua Prof. Dr. Antônio Celso Wagner Zanin 250 CEP São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic Rua José Bonifácio 1193 CEP São Paulo State University (UNESP) School of Dentistry Department of Restorative Dentistry Rua José Bonifácio 1193 CEP FAPESP: 2013/26779-4

Aim This study evaluated the effects of 5-fluorouracil (5-FU) and cisplatin (CIS) in healthy periodontal tissues and in the early stages of experimental periodontitis (EP) in rats. Methods One hundred and eighty male rats were divided into three groups, which were submitted to the following systemic treatments: physiological saline solution (PSS); CIS and 5FU. Each group was subdivided into two subgroups: without (NEP) and with (EP) induction of EP. Animals were euthanized at 3, 5 and 7 days post-treatment. Histological, histometric (percentage of bone in the furcation [PBF]) and immunohistochemical (for tumour necrosis factor-α, interleukin-1β and receptor activator of nuclear factor-κB ligand) analyses were performed. Data were statistically analysed. Results CIS-NEP and 5FU-NEP showed more inflammation than PSS-NEP at 3, 5 and 7 days. CIS-EP and 5FU-EP showed more inflammation and lower PBF than PSS-EP at all periods of evaluation. 5FU-EP showed lower PBF than CIS-EP at 5 and 7 days. Conclusion 5-FU and CIS exacerbated periodontal inflammation and aggravated the progression of EP in its early stages.

This study assesses the effects of topical sodium alendronate (SA) as an adjuvant to the mechanical treatment of ligature-induced periodontitis in rats.Ninety animals were subjected to the induction of periodontitis via the installation of a ligature around the mandibular left first molar. After 7 days, the ligature was removed, and the animals were distributed into the following groups: 1) NT group (n = 30), no treatment; 2) SRP group (n = 30), scaling and root planing (SRP) and local irrigation with physiologic saline solution; and 3) SRP/SA group (n = 30), SRP and local irrigation with SA (10(-5) M). Ten animals from each group were euthanized at 7, 15, and 30 days after treatment. Histologic and histometric analyses were performed in the furcation region. The percentage of bone in the furcation (PBF) was measured. Immunohistochemical analyses for detecting the receptor activator of nuclear factor-κB ligand (RANKL), osteoprotegerin (OPG), tartrate-resistant acid phosphatase (TRAP), and activated caspase-3 were performed at the furcation region.Compared with the other groups, the SRP/SA group showed less local inflammation and better tissue reparation during the entire experiment. There was more PBF in the SRP/SA group than in the other groups at days 7 and 15. Stronger OPG immunolabeling and weaker RANKL immunolabeling were observed in the SRP/SA group at 15 and 30 days. There were fewer TRAP-positive cells in the SRP/SA group than in the NT group at all of the time points. There was no difference in the number of activated caspase-3-positive osteocytes among groups and time points.It can be concluded that topical use of SA as an adjuvant to SRP is effective in the treatment of experimental periodontitis.

This study aimed to evaluate the progression and treatment of experimental periodontitis (EP) in rats treated with 5-fluorouracil (5-FU).One-hundred fifty rats were divided into groups: C--control, EP--EP induction, EP/scaling and root planing (SRP)--EP induction and SRP, 5-FU--systemic treatment with 5-FU, 5-FU/EP--systemic treatment with 5-FU and EP induction, and 5-FU/EP/SRP--systemic treatment with 5-FU, EP induction and SRP. Systemic treatment consisted of the administration of 80 and 40 mg/kg of 5-FU. The animals were euthanised at 7, 15 and 30 days. Histological and immunohistochemical analyses for the detection of the receptor activator of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP) were performed at the furcation region. A histometric analysis was performed to evaluate the percentage of bone in the furcation region (PBF).In the 5-FU/EP and 5-FU/EP/SRP groups, there was less PBF, greater breakdown of periodontal tissues and increased immunostaining for RANKL compared with the EP and EP/SRP groups.It can be concluded that 5-FU aggravates PE progression, impairs the host response to SRP mechanical therapy and does not seem to cause changes in the healthy periodontium.

This study evaluates the influence of platelet-rich plasma derived from bone marrow aspirate (PRP-BMA) on the healing of periodontal fenestration defects in rats.Periodontal fenestration defects were surgically created in the mandibles of 40 rats. The animals were randomly divided into two groups, control and PRP-BMA, in which defects were filled with blood clot or PRP-bma, respectively. Animals were euthanized at either 10 or 30 days post-surgery. Histologic, histometric, and immunohistochemical analyses were performed. Percentage of new bone area (NBA), area of bone trabeculae (ABT), new cementum (NC), and extension of remaining defect were histometrically evaluated. Proliferating cell nuclear antigen (PCNA), bone sialoprotein (BSP), osteocalcin (OCN), and tartrate-resistant acid phosphatase (TRAP) immunohistochemical staining were performed. Immunolabeled cells were quantified. Data were statistically analyzed (analysis of variance; Tukey, P0.05).At 10 days, control and PRP-BMA groups presented similar amounts of NBA and ABT; NC formation was not observed. At 30 days, control and PRP-BMA groups presented similar amounts of NBA and ABT; the PRP-BMA group showed NC formation with collagen fibers inserted obliquely or perpendicularly to the root surface. NC formation was not observed in any control group specimen. PRP- BMA presented higher numbers of PCNA-positive and BSP-positive cells than control at 10 and 30 days post-surgery. No significant differences in the number of either OCN-positive or TRAP-positive cells were observed between groups at 10 or 30 days.PRP-BMA promoted NC formation with a functional periodontal ligament when applied at experimental periodontal fenestration defects.

Made available in DSpace on 2018-12-11T17:32:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-10-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Purpose: The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) and antimicrobial photodynamic therapy (aPDT) as adjuvant to mechanical treatment of experimental periodontitis (EP) in adult rats submitted to 5-fluorouracil (5-FU) chemotherapy. Methods: EP was induced through ligature around the left mandibular first molar for 7 days. The ligature was removed and the animals separated into groups: EP, no treatment; 5FU, systemic administration of 5-FU (80 and 40 mg/kg); 5FU/scaling and root planing (SRP), systemic application of 5-FU and SRP; 5FU/SRP/LLLT, systemic application of 5-FU, SRP, and LLLT (660 nm, 0.035 W; 29.4 J/cm2); and 5FU/SRP/aPDT, systemic application of 5-FU, SRP, and aPDT (methylene blue irrigation and LLLT). The animals were euthanized 7, 15, and 30 days after treatments. Histological sections from mandibles were processed for histomorphometric and immunohistochemical analysis (TRAP, RANKL, OPG, TNF-α, IL-6, IL-10). The alveolar bone loss (BL) area in the furcation region of the mandibular first molar was analyzed histometrically. Results: There was less bone loss in 5FU/SRP/aPDT compared with 5FU at 7 days (p

Made available in DSpace on 2018-12-11T17:18:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01 Background: This study evaluated the antimicrobial photodynamic therapy (aPDT) as an adjunctive therapy to scaling and root planning (SRP) for the treatment of experimental periodontitis (EP) in ovariectomized rats under systemic nicotine. Methods: Female ovariectomized rats (n = 180) were divided into two groups: vehicle administration (Veh) and nicotine administration (Nic). Mini-pumps containing either vehicle or nicotine were inserted in the rats 30 days before the induction of EP, which was induced by placing a ligature around the left mandibular first molar. The rats were randomly divided into three treatment subgroups: SRP, SRP plus low-level laser therapy (LLLT), and SRP plus aPDT. aPDT consisted of the application of a phenothiazine photosensitizer followed by LLLT. Ten animals from each group were euthanized at days 7, 15, and 30 after periodontal treatment. The furcation region was evaluated using histological, histometric analyses and immunolabelling for PCNA, TRAP, RANKL, and OPG. Results: Nicotine administration resulted in greater bone loss (BL). aPDT resulted in lower BL compared to SRP. aPDT showed higher quantities of PCNA-positive cells compared to SRP, regardless of the nicotine status. aPDT resulted in less recruitment of osteoclasts and lower RANKL immunolabelling compared to LLLT and SRP. Conclusion: aPDT was effective in animals treated with nicotine. São Paulo State University (UNESP) Group for the Research and Study of Laser in Dentistry Department of Surgery and Integrated Clinic Division of Periodontics School of Dentistry, Araçatuba, 1193, Jose Bonifácio São Paulo State University (UNESP) Department of Basic Science School of Dentistry, Araçatuba, 1193, José Bonifácio São Paulo State University (UNESP) Group for the Research and Study of Laser in Dentistry Department of Surgery and Integrated Clinic Division of Periodontics School of Dentistry, Araçatuba, 1193, Jose Bonifácio São Paulo State University (UNESP) Department of Basic Science School of Dentistry, Araçatuba, 1193, José Bonifácio

The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) on the bone repair of critical size defects (CSDs) filled with autogenous bone in the calvaria of immunosuppressed rats. A 5 mm-diameter CSD was created in the calvaria of 30 rats. The animals were divided into 5 groups (n = 6): Control (C)--the defect was filled with a blood clot; Dexamethasone (D)--dexamethasone treatment, and the defect was filled with a blood clot; Autogenous bone (AB)--dexamethasone treatment, and the defect was filled with autogenous bone; LLLT--dexamethasone treatment, and the defect received LLLT (660 nm; 35 mW; 24.7 J/cm(2)); and AB + LLLT--dexamethasone treatment, and the defect was filled with autogenous bone and received LLLT. All animals were euthanized at 30 postoperative days. Histometric and histological analyses were performed. The new bone area (NBA) was calculated as the percentage of the total area of the original defect. Data were analysed statistically (an analysis of variance and Tukey's test; P < 0.05). The AB + LLLT group showed the largest NBA of all groups (P < 0.05). The use of LLLT with AB effectively stimulated bone formation in CSDs in the calvaria of immunosuppressed rats.

This study histomorphometrically analyzes the influence of platelet-rich plasma (PRP), low-level laser therapy (LLLT), or their combination on the healing of periodontal fenestration defects (PFDs) in rats.PFDs were surgically created in the mandibles of 80 rats. The animals were randomly divided into four groups: 1) C (control) and 2) PRP, defects were filled with blood clot or PRP, respectively; 3) LLLT and 4) PRP/LLLT, defects received laser irradiation, were filled with blood clot or PRP, respectively, and then irradiated again. Animals were euthanized at either 10 or 30 days post-surgery. Percentage of new bone (NB), density of newly formed bone (DNB), new cementum (NC), and extension of remaining defect (ERD) were histomorphometrically evaluated. Data were statistically analyzed (analysis of variance; Tukey test, P0.05).At 10 days, group PRP presented ERD significantly lower than group C. At 30 days, group PRP presented NB and DNB significantly greater than group C. Groups LLLT, PRP, and PRP/LLLT showed significant NC formation at 30 days, with collagen fibers inserted obliquely or perpendicularly to the root surface. NC formation was not observed in any group C specimen.LLLT, PRP, or their combination all promoted NC formation with a functional periodontal ligament. The combination PRP/LLLT did not show additional positive effects compared to the use of either therapy alone.

The aim of the present study was to evaluate the antimicrobial effect of antimicrobial photodynamic therapy (aPDT) in alveolar treatment of areas with induced periodon- titis. Thirty male Wistar rats were subjectedto ligature-induced periodontal disease (PD) in the first left inferior molars, while the right side molars did not receive ligatures. After 7 days of PD evolution, ligatures were removed from the left side, and the first left and right mandibular molars were extracted. Afterwards, animals were divided into groups according to the following treatments: control (C)—no treatment; mechan- ical debridement (MD)—mechanical debridement and irriga- tion with saline solution; and aPDT—mechanical debridement, irrigation with toluidine blue O (TBO), and 1 min of laser irradiation (GaAlAs, 660 nm, 30 mW, 32 J/cm 2 ,6 0 s). Liga- tures were removed and samples of the alveolar content after extraction and after each treatment were collected for microbial processing by real-time polymerase chain reaction with specific primers for Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis ,a ndTreponema denticola .D ata were submitted to statistical analysis by multiple comparison tests (McNemar test; p

Made available in DSpace on 2018-12-11T17:12:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01 Objective: The aim of this study was to investigate the biocompatibility of methylene blue at different pH levels through the method of implantation in subcutaneous tissue. Materials and methods: Eighty-four sterilized polyethylene tubes were allocated in the subcutaneous tissue of 28 rats, each one receiving four tubes, set into four groups: group tube (G-T)—empty tube, fibrin group (G-F)—tube filled with fibrin sponge, group methylene blue pH 7 (G-MB/pH 7)—tube filled with fibrin sponge soaked by methylene blue (100 μg/ml) at pH 7.0, and group methylene blue pH 1 (G-MB/pH 1)—tube filled with fibrin sponge and soaked by methylene blue (100 μg/ml) at pH 1.0. After 7, 15, and 30 days, seven animals from each group were euthanized, and the tubes involved by the surrounding tissue were removed and fixed with 4% buffered formaldehyde solution. The collected pieces were processed and histological sections (4 μm) were stained with hematoxylin and eosin and analyzed by light microscopy. Scores were assigned to analysis of histopathologic parameters. The results were statistically analyzed by the Kruskal–Wallis test (p ≤ 0.05). Results: At 7 and 30 days, the G-MB/pH 1 group showed no significant difference in the G-T control group, while G-MB/pH 7 had a significant increase on tissue reaction, also when compared to G-T. At 15 days, there was no statistical difference between the groups. Conclusion: Within the limits of this study, it is concluded that methylene blue at pH 1.0 provides better biocompatibility than at pH 7.0. Department of Surgery and Integrated Clinic Division of Periodontics São Paulo State University (Unesp) School of Dentistry, Araçatuba - R. José Bonifácio, 1193 Department of Endodontics São Paulo State University (Unesp) School of Dentistry, Araçatuba - R. José Bonifácio, 1193 Department of Surgery and Integrated Clinic Division of Periodontics São Paulo State University (Unesp) School of Dentistry, Araçatuba - R. José Bonifácio, 1193 Department of Endodontics São Paulo State University (Unesp) School of Dentistry, Araçatuba - R. José Bonifácio, 1193

Made available in DSpace on 2018-12-11T17:08:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-03-01 Objective To analyze the influence of low-level laser therapy (LLLT) on the bone healing process of autogenous bone block grafts installed in nicotine systemically modified rats. Methods Seventy-two rats (Wistar) were randomly assigned into 4 groups (n = 18). SS-BG: saline application + bone graft. SS-BG/LLLT: saline application + bone graft + LLLT. NIC-BG: nicotine application + bone graft. NIC-BG/LLLT: nicotine application + bone graft + LLLT. After 30 days of application of solutions, all animals received autogenous bone block graft in the jaw, with the donation from the parietal bone's calvarial area. Treatment with LLLT was in bed-graft interface, after accommodation of the graft. The animals in each group were sacrificed at 7, 14, and 28 days after graft surgery. Results The histologic analyses of NIC-BG group depicted a delay of osteogenic activity in the recipient bed-graft interface and the irradiation of tissue with LLLT provided better bone healing. The histometric analysis revealed that SS-BG/LLLT and NIC-BG/LLLT groups showed increased bone formation compared to BG-SS and NIC-BG groups, after 14 days (SS-BG 24.94% ± 13.06% versus SS-BG/LLLT 27.53% ± 19.07% and NIC-BG 14.27% ± 2.22% versus NIC-BG/LLLT 24.37% ± 11.93%) and 28 days (SS-BG 50.31% ± 2.69% versus SS-BG/LLLT 58 19% ± 12.32% and NIC-BG 36.89% ± 8.40% versus NIC-BG/LLLT 45.81% ± 6.03%). Conclusion Nicotine harms bone formation in the bed-graft interface and LLLT action can mitigate this. Univ. Estadual Paulista-UNESP Division of Periodontics Department of Surgery and Integrated Clinic Dental School of Araçatuba, Rua José Bonifácio, n° 1193, São Paulo Univ. Estadual Paulista-UNESP Division of Periodontics Department of Surgery and Integrated Clinic Dental School of Araçatuba, Rua José Bonifácio, n° 1193, São Paulo

Low-level laser (LLL) has been used on peri-implant tissues for accelerating bone formation. However, the effect of one session of LLL in the strength of bone-implant interface during early healing process remains unclear. The present study aims to evaluate the removal torque of titanium implants irradiated with LLL during surgical preparation of implant bed, in comparison to non-irradiation. Sixty-four Wistar rats were used. Half of the animals were included in LLL group, while the other half remained as control. All animals had the tibia prepared with a 2 mm drill, and a titanium implant (2.2 × 4 mm) was inserted. Animals from LLL group were irradiated with laser (gallium aluminum arsenide), with a wavelength of 808 nm, a measured power output of 50 mW, to emit radiation in collimated beams (0.4 cm(2)), for 1 min and 23 s, and an energy density of 11 J/cm(2). Two applications (22 J/cm(2)) were performed immediately after bed preparation for implant installation. Flaps were sutured, and animals from both groups were sacrificed 7, 15, 30, and 45 days after implant installation, when load necessary for removing implant from bone was evaluated by using a torquimeter. In both groups, torque values tended to increase overtime; and at 30 and 45 days periods, values were statistically higher for LLL group in comparison to control (ANOVA test, p0.0001). Thus, it could be suggested that a single session of irradiation with LLL was beneficial to improve bone-implant interface strength, contributing to the osseointegration process.

The purpose of this study was to analyze histologically the effect of low-level laser therapy (LLLT) in combination with bisphosphonate on bone healing in surgically created critical size defects (CSD) in rat calvaria. One hundred Wistar female rats sham operated (sham) and ovariectomized (Ovx) were maintained untreated for 1 month to allow for the development of osteopenia in the Ovx animals. A CSD was made in the calvarium of each rat, and the animals were divided into five groups according to following treatments: (1) sham rats (control), (2) Ovx rats, (3) Ovx rats treated with LLLT, (4) Ovx rats treated with bisphosphonate, and (5) Ovx rats treated with bisphosphonate and LLLT. Groups 4 and 5 were irrigated with 1 ml of bisphosphonate, and groups 3 and 5 were submitted to LLLT (GaAlAs), 660 nm, 24 J, and 0.4285 W/cm(2) on the CSD. Ten animals of each treatment were killed at 30 and 60 days. Histomorphometric assessments, using image analysis software, and histological analyses were performed. No defect was completely regenerated with the bone. Histometrically, it can be observed that groups 3 (37.49 ± 1.94%, 43.11 ± 2.39%) and 5 (35.05 ± 1.57%, 41.07 ± 1.89%) showed a significant bone neoformation when compared to groups 1 (16.81 ± 1.57%, 27.54 ± 1.49%), 2 (11.68 ± 0.98%, 22.51 ± 1.05%), and 4 (14.62 ± 1.70%, 25.67 ± 1.41%) in all experimental periods (P 0.05). It was possible to conclude that the LLLT associated or not with bisphosphonate treatment was effective for stimulating bone formation in CSD in the calvaria of rats submitted to ovariectomy.