The influence of antineoplastic agents on the peri-implant bone around osseointegrated titanium implants: an in vivo histomorphometric and immunohistochemical study

Made available in DSpace on 2022-05-01T09:47:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background and objective: The interaction between antineoplastic drugs used for treating cancer and non-affected tissues remains poorly assessed and may be critical for maintaining the quality of life for patients during and after treatment. This pre-clinical study evaluated the effects of cisplatin (CIS) and 5-fluorouracil (5-FU) on the peri-implant repair process around osseointegrated titanium implants installed in the tibiae of rats. Material and methods: Were used 90 male rats, randomly divided into three groups (n = 30): physiological saline solution (PSS), CIS, and 5-FU. Titanium implants (4.0 × 2.2 mm) were inserted in both tibiae of all animals at day 0. The animals received either PSS, CIS, or 5-FU at 35 and 37 days. Euthanasia was performed at 50, 65, and 95 days after surgery. Histometric (bone/implant contact [BIC]) and bone area fraction occupancy (% BAFO), histological, and immunohistochemical (for bone morphogenetic protein 2/4 [BMP2/4], Runt-related transcription factor 2 [RUNX2], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analyses were performed. Data were statistically analyzed. Results: Groups CIS and 5-FU presented lower BIC and lower BAFO as compared with PSS in all time points. The imbalance in bone turnover was observed by the lower number of BMP2/4-, RUNX2-, and OCN-positive cells/mm2 and the higher number of TRAP-positive cells/mm in groups CIS and 5-FU as compared with PSS in all time points. Persistent and exacerbated inflammation was observed in groups CIS and 5-FU. Conclusions: Both antineoplastic agents interfered negatively in the bone turnover around osseointegrated titanium implants. Clinical relevance: Closer and more careful follow-up of patients with osseointegrated implants that will undergo chemotherapy with either CIS or 5-FU shall be performed. Department of Diagnosis and Surgery–Periodontics Division São Paulo State University (Unesp), St. José Bonifácio 1193 - Vila Mendonça, SP Nucleus of Study and Research in Periodontics and Implantology (NEPPI) School of Dentistry São Paulo State University (Unesp), SP Department of Basic Science School of Dentistry São Paulo State University (Unesp), SP Department of Diagnosis and Surgery–Periodontics Division São Paulo State University (Unesp), St. José Bonifácio 1193 - Vila Mendonça, SP Nucleus of Study and Research in Periodontics and Implantology (NEPPI) School of Dentistry São Paulo State University (Unesp), SP Department of Basic Science School of Dentistry São Paulo State University (Unesp), SP FAPESP: 2014/11427-8 FAPESP: 2017/11805-0