1. Vitamin D metabolites in human milk
- Author
-
Zipora Eisenberg, Zvi Spirer, Yosef Weisman, and Joseph C. Bawnik
- Subjects
Chromatography ,Milk, Human ,Hydroxycholecalciferols ,Ultraviolet Rays ,business.industry ,Metabolism ,High-performance liquid chromatography ,Biological materials ,Radioligand Assay ,Vitamin D+Metabolites ,Biochemistry ,Sephadex ,Pediatrics, Perinatology and Child Health ,Dihydroxycholecalciferols ,Vitamin D and neurology ,Humans ,Medicine ,Uv detection ,business ,Chromatography, High Pressure Liquid - Abstract
The concentrations of unconjugated 25-OHD, 24, 25(OH)2D, and 1,25(OH)2D were measured in human milk by competitive protein-binding radioassays following successive preparative Sephadex LH-20 chromatography and HPLC. The mean (+/- SE) concentration of 25-OHD was 0.37 +/- 0.03 ng/ml, of 24,25(OH)2D was 24.8 +/- 1.9 pg/ml, and of 1,25(OH)2D was 2.2 +/-0.1 pg/ml. The concentration of 25-OHD3 in milk as determined by HPLC and UV detection at 254 nm was 0.27 +/- 0.08 ng/ml. The milk concentrations of vitamin D metabolites did not correlate with the maternal serum 25-OHD levels. The total amounts of unconjugated vitamin D metabolites correspond to the known low bioassayable vitamin D antirachitic activity in human milk.
- Published
- 1982