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Vitamin D metabolites in human milk
- Source :
- The Journal of Pediatrics. 100:745-748
- Publication Year :
- 1982
- Publisher :
- Elsevier BV, 1982.
-
Abstract
- The concentrations of unconjugated 25-OHD, 24, 25(OH)2D, and 1,25(OH)2D were measured in human milk by competitive protein-binding radioassays following successive preparative Sephadex LH-20 chromatography and HPLC. The mean (+/- SE) concentration of 25-OHD was 0.37 +/- 0.03 ng/ml, of 24,25(OH)2D was 24.8 +/- 1.9 pg/ml, and of 1,25(OH)2D was 2.2 +/-0.1 pg/ml. The concentration of 25-OHD3 in milk as determined by HPLC and UV detection at 254 nm was 0.27 +/- 0.08 ng/ml. The milk concentrations of vitamin D metabolites did not correlate with the maternal serum 25-OHD levels. The total amounts of unconjugated vitamin D metabolites correspond to the known low bioassayable vitamin D antirachitic activity in human milk.
- Subjects :
- Chromatography
Milk, Human
Hydroxycholecalciferols
Ultraviolet Rays
business.industry
Metabolism
High-performance liquid chromatography
Biological materials
Radioligand Assay
Vitamin D+Metabolites
Biochemistry
Sephadex
Pediatrics, Perinatology and Child Health
Dihydroxycholecalciferols
Vitamin D and neurology
Humans
Medicine
Uv detection
business
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 00223476
- Volume :
- 100
- Database :
- OpenAIRE
- Journal :
- The Journal of Pediatrics
- Accession number :
- edsair.doi.dedup.....ced6a6950655b24c2e767c3430eb911a