1. Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
- Author
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Nissim Benvenisty, Minal Patel, Glyn Stacey, Lesley Young, Sanna Vuoristo, Barak Blum, Robert Passier, Steve Oh, Rebecca S. Hamilton, Miodrag Stojkovic, Barbara B. Knowles, Benjamin Reubinoff, Kehkooi Kee, Barbara S. Mallon, Andras Nagy, Michal Amit, Janet Rossant, Paul J. Gokhale, Franck Lebrin, Henrike Siemen, Simon Bevan, Weidong Zhang, Milla Mikkola, Stephen L. Minger, Norio Nakatsuji, Peter W. Andrews, Eric S Sherrer, Paul Bello, Tikva Turetsky, Lorraine S. Berry, Behrouz Aflatoonian, Christine L. Mummery, Masha Mileikovsky, Yoav Mayshar, Angela Ford, Harry Moore, Mark Jones, Joseph Itskovitz-Eldor, Jackie Johnson, Lars Ährlund-Richter, Roland A. Fleck, Oluseun Adewumi, Jamie P. Jackson, Alex Hampl, Andre Bh Choo, Petr Dvorak, Timo Otonkoski, Daisy Manning, Henrik Semb, Marta P. Imreh, Justin Brooking, Marina Gertsenstein, Dorien Ward, Jin P. Szatkiewicz, Timo Tuuri, Thomas C Schulz, Marian S Piekarczyk, Kevin G. Chen, Karin Astrid Maria Gertow, Renee A. Reijo Pera, Thomas A Weaver, Gemma Beighton, Kye-Yoon Park, Majlinda Lako, Ronald D.G. McKay, Katarina Emanuelsson, Cia Olsson, Carmel M. O’Brien, Johan Hyllner, Hema Patel, Outi Hovatta, Allan J. Robins, Hirofumi Suemori, Peter J. Rugg-Gunn, Martin F. Pera, Marie Corbel, Gary A. Churchill, Ivan Damjanov, John S Draper, Benjamin L. King, Lyn Healy, Steineke van den Brink, Roger A. Pedersen, Anna E. Michalska, and Kristina Vintersten
- Subjects
Homeobox protein NANOG ,Genotype ,Cellular differentiation ,Biomedical Engineering ,Bioengineering ,Biology ,Stem cell marker ,Applied Microbiology and Biotechnology ,Tetraspanin 29 ,03 medical and health sciences ,0302 clinical medicine ,Antigens, CD ,Cluster Analysis ,Humans ,Cell Lineage ,CD90 ,Cells, Cultured ,Embryonic Stem Cells ,030304 developmental biology ,0303 health sciences ,Membrane Glycoproteins ,Gene Expression Profiling ,Lineage markers ,Cell Membrane ,Gene Expression Regulation, Developmental ,Cell Differentiation ,Alkaline Phosphatase ,Molecular biology ,Embryonic stem cell ,3. Good health ,030220 oncology & carcinogenesis ,embryonic structures ,Molecular Medicine ,Female ,XIST ,Glycolipids ,Stem cell ,Biotechnology - Abstract
The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.
- Published
- 2007