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Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
- Source :
- Nature Biotechnology. 25:803-816
- Publication Year :
- 2007
- Publisher :
- Springer Science and Business Media LLC, 2007.
-
Abstract
- The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.
- Subjects :
- Homeobox protein NANOG
Genotype
Cellular differentiation
Biomedical Engineering
Bioengineering
Biology
Stem cell marker
Applied Microbiology and Biotechnology
Tetraspanin 29
03 medical and health sciences
0302 clinical medicine
Antigens, CD
Cluster Analysis
Humans
Cell Lineage
CD90
Cells, Cultured
Embryonic Stem Cells
030304 developmental biology
0303 health sciences
Membrane Glycoproteins
Gene Expression Profiling
Lineage markers
Cell Membrane
Gene Expression Regulation, Developmental
Cell Differentiation
Alkaline Phosphatase
Molecular biology
Embryonic stem cell
3. Good health
030220 oncology & carcinogenesis
embryonic structures
Molecular Medicine
Female
XIST
Glycolipids
Stem cell
Biotechnology
Subjects
Details
- ISSN :
- 15461696 and 10870156
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Nature Biotechnology
- Accession number :
- edsair.doi.dedup.....867a067cc25cab7204dd3712d4e89593