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The presence of methicillin-resistant Staphylococcus aureus (MRSA) strains in food products is a major issue for food safety. The present study was conducted to evaluate the occurrence and antimicrobial resistance profile of S. aureus, focusing on MRSA isolates, in ready-to-eat sashimi from Japanese restaurants in Salvador, Brazil. A total of 127 sashimi samples were collected directly from the take-out service in 16 restaurants. The staphylococcal isolates were identified morphologically and biochemically with standard laboratory procedures. S. aureus isolates were tested with a disk diffusion assay against seven antibiotics, and the cefoxitin and oxacillin were used to identify MRSA strains. Isolates with the MRSA phenotype were confirmed with a PCR assay. S. aureus was found in 73% of the sashimi samples, including sashimi from tuna (75.5% of samples) and salmon (72.5% of samples). Among those positive samples, 37% were contaminated with MRSA strains, found among 38.8% of salmon sashimi and 34.0% of tuna sashimi. Penicillin resistance was the most common type of antimicrobial resistance, found in 65.5% of the sashimi samples, followed by resistance to tetracycline (22.5%), erythromycin (16.0%), and ciprofloxacin (3.2%). Only two S. aureus isolates collected from different fish samples and restaurants had presumed resistance to vancomycin. The high prevalence of S. aureus and MRSA in these sashimi samples indicates a potential risk for foodborne disease, especially MRSA, spreading in the community. HIGHLIGHTS

The aims of this study were to evaluate the presence of methicillin-resistant Staphylococcus aureus on the hands and anterior nares of food handlers in public hospitals in the city of Salvador, Bahia, in northeastern Brazil and to evaluate the effectiveness of antiseptics for controlling contamination. Swabs from the nose and hands were collected from 140 food handlers in ten public hospitals, and methicillin-resistant S aureus (MRSA) isolates were confirmed by assessing their growth on selective media, coagulase testing and evaluating their antibiotic susceptibilities. Seventy (50.0%) food handlers were colonized with coagulase-positive Staphylococci on their hands and/or nares, and 40 (28.6%) food handlers were colonized with MRSA. The evaluation of susceptibility to the most commonly used anti-MRSA drugs demonstrated that 72.9% of the isolates from the handlers' hands and 82.5% of the isolates from the anterior nares showed resistance to vancomycin. The presence of MRSA was not correlated with the specific job function of the food handlers (p > 0.05). The logistic regression analysis of the antimicrobial activity of antiseptics against MRSA isolates indicated that 2% chlorhexidine had a significantly higher removal rate than those of alcohol gel and 10% PVP-I (iodophor) (p

This study investigated the presence of methicillin-resistant Staphylococcus aureus (MRSA) in raw meat and fish and foods prepared from them for patient consumption in public hospitals in Salvador, Bahia, in northeastern Brazil. A total of 114 samples of raw meat and fish (chicken, n = 30; beef, n = 30; pork, n = 24; and fish, n = 30) and 63 samples of prepared foods (made with chicken, n = 15; beef, n = 15; pork, n = 15; and fish, n = 18) were collected from the kitchens of 10 different hospitals. Of the 114 investigated raw meat and fish samples, 28.1% were positive for MRSA, which comprised 23.3% beef, 23.3% chicken, 37.5% pork, and 30% fish samples. Of the prepared foods, 9.5% were positive for MRSA, which comprised 5.6% chicken products, 6.7% pork products, and 22.2% fish products. MRSA contamination was not detected in prepared beef dishes. A statistical analysis showed no association between the presence of MRSA and the type of raw food (P0.05). The high prevalence of MRSA among the raw foods tested and the presence of the microorganism in prepared foods emphasizes the necessity of enforcing hygienic practices within hospital kitchens.

Texto completo: Acesso restrito. p. 26 - 213 Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-01-16T20:12:56Z No. of bitstreams: 1 aaa5959.pdf: 282927 bytes, checksum: c49f686f030ca8a05cde6dabcd49fb2e (MD5) Made available in DSpace on 2013-01-16T20:12:56Z (GMT). No. of bitstreams: 1 aaa5959.pdf: 282927 bytes, checksum: c49f686f030ca8a05cde6dabcd49fb2e (MD5) Previous issue date: 2012 The aim of this study was to evaluate the level of knowledge, attitudes and practices in food safety and the presence of coagulase-positive staphylococci species on the hands of food handlers in the municipal schools of Camaçari, Bahia in northeast Brazil. This study was conducted in two stages. In the first stage, 166 food handlers were interviewed via a questionnaire to assess their food safety knowledge, attitudes and practices. In the second phase, the presence of coagulase-positive staphylococci species on the hands of food handlers was determined. The results indicated that most of the handlers had been trained (92.2%), but the level of knowledge was insufficient; the average proficiency score was

Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2017-06-05T16:41:22Z No. of bitstreams: 1 3_v.9_3.pdf: 439530 bytes, checksum: b92959215ab6fcc52a84cfab8f2a719c (MD5) Made available in DSpace on 2017-06-05T16:41:22Z (GMT). No. of bitstreams: 1 3_v.9_3.pdf: 439530 bytes, checksum: b92959215ab6fcc52a84cfab8f2a719c (MD5) Previous issue date: 2010-09 O consumo de hortaliças folhosas, em especial a Lactuca sativa (alface), tem aumentado devido à crescente preocupação em se obter uma alimentação mais saudável. Entretanto, quando contaminadas, elas são responsáveis pela transmissão de grande número de doenças infecciosas. Este trabalho tem como objetivo avaliar as condições sanitárias ambientais de hortaliças comercializadas nos municípios de Catu e Alagoinhas (Bahia, Brasil). Foi realizada uma avaliação ambiental para detectar possíveis fontes de contaminação das hortas. Após serem coletadas as amostras de alfaces e da água de irrigação, elas foram levadas para análises parasitológicas (técnica de sedimentação) e análises microbiológicas (número mais provável) na Universidade do Estado da Bahia (Campus II). As águas de irrigação obtidas eram provenientes de rios, nascentes e poços, sendo utilizados, como material de adubação, esterco de galinha e bovino, não existindo esgotamento sanitário nas hortas estudadas. As análises parasitológicas da alface mostraram 20% de contaminação por ancilostomídeos. Quando avaliado o grau microbiológico, os resultados mostram que 100% são positivos para coliformes na alface e 88,9% positivos para coliformes na água de irrigação, em desacordo, portanto, com a legislação vigente. Salvador

Foodborne diseases have been increasing in recent years, with a greater impact on the health and economy of the developing countries than on the developed countries. The aim of this study was to evaluate the level of knowledge, attitudes and practices in food safety of food handlers in public hospitals of Salvador, Bahia. This is a descriptive, exploratory and transversal study, conducted with 237 food handlers from ten public hospitals of Salvador, Bahia. The data were collected through a structured questionnaire to assess the food handlers safety knowledge, attitudes and practices. The results indicated that most of the handlers had been trained (92.2%), but their level of knowledge was insufficient. Attitude received the highest scores, 98.3% accuracy and practices were sufficient 73.4% of the handlers. Logistic regression analysis showed association between knowledge and the level of education (p 0.05). It seems that more specific training courses should be planned for food handlers.

As bactérias do grupo coliformes são indicadoras de contaminação fecal, sendo empregadas como parâmetro para caracterização da qualidade das águas em geral, bem como monitoramento daquelas destinadas ao consumo humano. O objetivo deste trabalho foi avaliar a qualidade bacteriológica de água mineral. Foram analisadas sete diferentes marcas comercializadas no município de Alagoinhas (BA), no período de junho de 2008 a junho de 2009, pelo método de tubos múltiplos, para a determinação do número mais provável (NMP/100 mL) de coliformes totais e termotolerantes, contagem padrão e coloração de Gram, para análise das características morfotintoriais das bactérias mesofílicas. As análises foram realizadas no Laboratório de Biologia Experimental da Universidade do Estado da Bahia (UNEB), Campus II, Alagoinhas. Nos resultados obtidos, três amostras apresentaram contaminação por coliformes totais e, dentre estas, duas revelaram contaminação por coliformes termotolerantes. Na contagem de colônias, três amostras deram resultados insatisfatórios com mais de 500 UFC/mL. Verificou-se a presença de bactérias Gram positivas e Gram negativas, cocos e bacilos. Estes resultados mostram que 57,1% das amostras encontram-se em desacordo com um ou mais padrões e são contraindicadas para consumo humano.