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Occurrence of Methicillin-Resistant Staphylococcus aureus in Ready-to-Eat Raw Fish from Japanese Cuisine Restaurants in Salvador, Brazil
- Source :
- Journal of food protection. 83(6)
- Publication Year :
- 2019
-
Abstract
- The presence of methicillin-resistant Staphylococcus aureus (MRSA) strains in food products is a major issue for food safety. The present study was conducted to evaluate the occurrence and antimicrobial resistance profile of S. aureus, focusing on MRSA isolates, in ready-to-eat sashimi from Japanese restaurants in Salvador, Brazil. A total of 127 sashimi samples were collected directly from the take-out service in 16 restaurants. The staphylococcal isolates were identified morphologically and biochemically with standard laboratory procedures. S. aureus isolates were tested with a disk diffusion assay against seven antibiotics, and the cefoxitin and oxacillin were used to identify MRSA strains. Isolates with the MRSA phenotype were confirmed with a PCR assay. S. aureus was found in 73% of the sashimi samples, including sashimi from tuna (75.5% of samples) and salmon (72.5% of samples). Among those positive samples, 37% were contaminated with MRSA strains, found among 38.8% of salmon sashimi and 34.0% of tuna sashimi. Penicillin resistance was the most common type of antimicrobial resistance, found in 65.5% of the sashimi samples, followed by resistance to tetracycline (22.5%), erythromycin (16.0%), and ciprofloxacin (3.2%). Only two S. aureus isolates collected from different fish samples and restaurants had presumed resistance to vancomycin. The high prevalence of S. aureus and MRSA in these sashimi samples indicates a potential risk for foodborne disease, especially MRSA, spreading in the community. HIGHLIGHTS
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
Veterinary medicine
Staphylococcus aureus
Restaurants
medicine.drug_class
Antibiotics
Erythromycin
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Microbiology
03 medical and health sciences
Antibiotic resistance
Japan
medicine
Animals
Cefoxitin
030304 developmental biology
0303 health sciences
030306 microbiology
biochemical phenomena, metabolism, and nutrition
Staphylococcal Infections
Methicillin-resistant Staphylococcus aureus
Anti-Bacterial Agents
Ciprofloxacin
Vancomycin
Brazil
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 19449097
- Volume :
- 83
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of food protection
- Accession number :
- edsair.doi.dedup.....21ae24d0d2d698e9d53c6733dd1fa26a