71 results on '"Jing-Han, Wang"'
Search Results
2. π-Electron-Extended Triazine-Based Covalent Organic Framework as Photocatalyst for Organic Pollution Degradation and H2 Production from Water
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Jing Han Wang, Taher A. Gaber, Shiao-Wei Kuo, and Ahmed F. M. EL-Mahdy
- Subjects
covalent organic framework ,rhodamine B ,dye removal ,photodegradation ,photocatalysis H2 evolution ,Organic chemistry ,QD241-441 - Abstract
Herein, we report the efficient preparation of π-electron-extended triazine-based covalent organic framework (TFP-TPTPh COF) for photocatalysis and adsorption of the rhodamine B (RhB) dye molecule, as well as for photocatalytic hydrogen generation from water. The resultant TFP-TPTPh COF exhibited remarkable porosity, excellent crystallinity, high surface area of 724 m2 g−1, and massive thermal stability with a char yield of 63.41%. The TFP-TPTPh COF demonstrated an excellent removal efficiency of RhB from water in 60 min when used as an adsorbent, and its maximum adsorption capacity (Qm) of 480 mg g−1 is among the highest Qm values for porous polymers ever to be recorded. In addition, the TFP-TPTPh COF showed a remarkable photocatalytic degradation of RhB dye molecules with a reaction rate constant of 4.1 × 10−2 min−1 and an efficiency of 97.02% under ultraviolet–visible light irradiation. Furthermore, without additional co-catalysts, the TFP-TPTPh COF displayed an excellent photocatalytic capacity for reducing water to generate H2 with a hydrogen evolution rate (HER) of 2712 μmol g−1 h−1. This highly active COF-based photocatalyst appears to be a useful material for dye removal from water, as well as solar energy processing and conversion.
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- 2023
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3. Donor–acceptor hetero[6]radialene-based three-dimensional covalent organic frameworks for organic pollutant adsorption, photocatalytic degradation, and hydrogen production activity.
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Jing Han Wang, Hassan, Ahmed E., Elewa, Ahmed M., and EL-Mahdy, Ahmed F. M.
- Abstract
Covalent organic frameworks (COFs) are promising photocatalysts for treating organic pollutants and solar-to-hydrogen conversion; however, their suboptimal energy band structure and rapid charge recombination limit their photocatalytic performance. Herein, we synthesized two donor–acceptor hetero[6]radialene-based TFP 3D COFs, TFP-Py and TFP-BF, with a unique [3,4]-linked ffc topological structure for the adsorptive and photocatalytic degradation of the rhodamine B (RhB) dye and photocatalytic hydrogen production from water. The acceptor is hetero[6]radialene and the donors are planar pyrene (Py) and twisted bifluorenylidene (BF). TFP 3D COFs surpass previously reported COFs in removing RhB from water in 10 min with a maximum adsorption capacity (Q
m ) of 840 mg g−1 . Interestingly, the incorporation of the twisted BF structure into the hetero[6]radialene-based 3D COF backbone improved the band gap structure, charge transport, and photoinduced electron and hole isolation. The TFP-BF 3D COF degrades organic dye (RhB) with 1.4-fold greater catalytic activity (1.5 × 10−2 min−1 ) than the planar pyrene donor-derived TFP-Py 3D COF (1.1 × 10−2 min−1 ). It demonstrates a hydrogen evolution (HER) rate of 21.04 mmol g−1 h−1 , obviating the need for a Pt cocatalyst, the highest COF-specific capacity ever recorded. This study illuminates how electron donor topology and hetero[6]radialene 3D structure affect photocatalysis. [ABSTRACT FROM AUTHOR]- Published
- 2024
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4. Research on the Pressure drop Characteristics of Spiral flow in Horizontal Straight Pipe.
- Author
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Xiao-Qian Huo, Ying Xu, Tao Zhang 0072, Jing-Han Wang, and Huaxiang Wang
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- 2019
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5. Water volume fraction measurement with hydrocyclone-capacitor sensor in horizontal gas-liquid swirling flow.
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Ying Xu, Chen Bian, Tao Zhang 0072, Jing-Han Wang, and Yi-Guang Yang
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- 2019
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6. Whole-Transcriptome Sequencing Identifies Key Differentially Expressed mRNAs, miRNAs, lncRNAs, and circRNAs Associated with CHOL
- Author
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Kai-Jian Chu, Yu-Shui Ma, Xiao-Hui Jiang, Ting-Miao Wu, Zhi-Jun Wu, Zhi-Zhen Li, Jing-Han Wang, Qing-Xiang Gao, Bin Yi, Yi Shi, Hui-Min Wang, Li-Peng Gu, Su-Qing Zhang, Gao-Ren Wang, Ji-Bin Liu, Da Fu, and Xiao-Qing Jiang
- Subjects
CHOL ,DEG ,ceRNA ,spliceosome ,miR-144-3p ,Therapeutics. Pharmacology ,RM1-950 - Abstract
To systematically evaluate the whole-transcriptome sequencing data of cholangiocarcinoma (CHOL) to gain more insights into the transcriptomic landscape and molecular mechanism of this cancer, we performed whole-transcriptome sequencing based on the tumorous (C) and their corresponding non-tumorous adjacent to the tumors (CP) from eight CHOL patients. Subsequently, differential expression analysis was performed on the C and CP groups, followed by functional interaction prediction analysis to investigate gene-regulatory circuits in CHOL. In addition, The Cancer Genome Atlas (TCGA) for CHOL data was used to validate the results. In total, 2,895 differentially expressed messenger RNAs (dif-mRNAs), 56 differentially expressed microRNAs (dif-miRNAs), 151 differentially expressed long non-coding RNAs (dif-lncRNAs), and 110 differentially expressed circular RNAs (dif-circRNAs) were found in CHOL samples compared with controls. Enrichment analysis on those differentially expressed genes (DEGs) related to miRNA, lncRNA, and circRNA also identified the function of spliceosome. The downregulated hsa-miR-144-3p were significantly enriched in the competing endogenous RNA (ceRNA) complex network, which also included 7 upregulated and 13 downregulated circRNAs, 7 upregulated lncRNAs, and 90 upregulated and 40 downregulated mRNAs. Moreover, most of the DEGs and a few of the miRNAs (such as hsa-miR-144-3p) were successfully validated by TCGA data. The genes involved in RNA splicing and protein degradation processes and miR-144-3p may play fundamental roles in the pathogenesis of CHOL.
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- 2020
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7. Long Noncoding RNA OIP5-AS1 Promotes the Progression of Liver Hepatocellular Carcinoma via Regulating the hsa-miR-26a-3p/EPHA2 Axis
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Yu-Shui Ma, Kai-Jian Chu, Chang-Chun Ling, Ting-Miao Wu, Xu-Chao Zhu, Ji-Bin Liu, Fei Yu, Zhi-Zhen Li, Jing-Han Wang, Qing-Xiang Gao, Bin Yi, Hui-Min Wang, Li-Peng Gu, Liu Li, Lin-Lin Tian, Yi Shi, Xiao-Qing Jiang, Da Fu, and Xiong-Wen Zhang
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LIHC ,OIP5-AS1 ,hsa-miR-26a-3p ,EPHA2 ,prognosis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Numerous studies have suggested that dysregulated long noncoding RNAs (lncRNAs) contributed to the development and progression of many cancers. lncRNA OIP5 antisense RNA 1 (OIP5-AS1) has been reported to be increased in several cancers. However, the roles of OIP5-AS1 in liver hepatocellular carcinoma (LIHC) remain to be investigated. In this study, we demonstrated that OIP5-AS1 was upregulated in LIHC tissue specimens and its overexpression was associated with the poor survival of patients with LIHC. Furthermore, loss-of function experiments indicated that OIP5-AS1 promoted cell proliferation and inhibited cell apoptosis both in vitro and in vivo. Moreover, binding sites between OIP5-AS1 and hsa-miR-26a-3p as well as between hsa-miR-26a-3p and EPHA2 were confirmed by luciferase assays. Finally, a rescue assay was performed to prove the effect of the OIP5-AS1/hsa-miR-26a-3p/EPHA2 axis on LIHC cell biological behaviors. Based on all of the above findings, our results suggested that OIP5-AS1 promoted LIHC cell proliferation and invasion via regulating the hsa-miR-26a-3p/EPHA2 axis.
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- 2020
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8. CPT1B, a metabolic molecule, is also an independent risk factor in CN-AML
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Fenglin Li, Shujuan Huang, Jie Jin, Wenle Ye, Yu Qian, Shihui Mao, Qing Ling, Jiansong Huang, Wenwen Wei, Xiangjie Lin, Jing-Han Wang, and Jiajia Pan
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Cancer Research ,Oncology ,business.industry ,Cancer research ,Genetics ,Medicine ,Molecule ,General Medicine ,Risk factor (computing) ,business - Abstract
Background: In recent years, fatty acid oxidation has been considered as an important energy source for tumorigenesis and development. There are extensive studies on CPT1A, a kind of fatty acid oxidation rate-limiting enzyme. However, prognostic value and regulatory network of another subtype CPT1B in AML remains elusive. This submission aims to clarify the independent prognostic role of the metabolic molecule CPT1B in CN-AML from cilinical data and molecule levels including mRNA, miRNA and lncRNA. Method: First, we analysed the CPT1B expression in AML conhort via online database “GEPIA”. Following the assessments on differential mRNA, miRNA, lncRNA expression analysis on the paired CPT1B high and low expression groups, there were abnormal changes at different molecular levels. Moreover, we determine the preliminary predictions on the regulatory network of CPT1B in AML by constructing miRNA-mRNA and ceRNA networks. The study had yielded a series of molecules that potentially proved the prognostic prediction and metabolic function of CPT1B from the side. Finally, we analysed the CPT1B expression in our own conhort to make a verification. Result: Notably, there was a significantly high expression of CPT1B in AML patients than normal people and was associated with the poor outcome. Molecules from different levels verified our finding and revealed the possible regulatory mechanism of CPT1B in AML. Conclusion: CPT1B is a potential prognostic factor as well as a therapeutic target of AML.
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- 2023
9. Differences between attached and suspended microalgal cells in ssPBR from the perspective of physiological properties
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Lin-Lan, Zhuang, Jing-Han, Wang, and Hong-Ying, Hu
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- 2018
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10. MiR-362-5p as a novel prognostic predictor of cytogenetically normal acute myeloid leukemia
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Qiu-Ling Ma, Jing-Han Wang, Min Yang, Huan-ping Wang, and Jie Jin
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miR-362-5p ,Acute myeloid leukemia ,Gene expression ,Prognosis ,Medicine - Abstract
Abstract Background MicroRNAs are of special interest in cancer research and hold significant promise as diagnostic and prognostic biomarkers for malignant disease. MiR-362-5p have been found to exert both oncogenic and tumor suppressive effects depending highly on the cellular context. The aim of this study was to determine whether the expression of miR-362-5p can be served as a prognostic factor for patients with cytogentically normal acute myeloid leukemia (CN-AML). Methods We enrolled 224 patients with CN-AML and measured the expression of miR-362-5p by quantitative real time PCR analysis. We classified patients into high and low expression based on the median value. The Cox regression analyses were carried out to assess the prognostic significance of miR-362-5p expression in the context of the well-established predictors. Additionally, microRNA expression profiling were conducted to identify the biological insights between high and low group. Results High expressers had older age. High expressers obtained shorter overall survival in the univariate analysis. The independent prognostic value of miR-362-5p remained in the context of the well-established clinical and cytogenetic predictors. Moreover, the prognostic value of miR-362-5p was also validated in an independent cohort of CN-AML. Notably, numerous oncomiRs were also high expressed in high miR-362-5p group. Conclusion High miR-362-5p expression was associated with poorer overall survival implicating the oncogenic function in AML development.
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- 2018
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11. Transition from isotropic to polar state of self-driven eccentric disks
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Jing-han Wang, Tian-liang Xu, Jing-xi He, Kang Chen, and Wen-de Tian
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General Physics and Astronomy - Abstract
Inspired by the eccentricity design of self-driven disks, we propose a computational model to study the remarkable behavior of this kind of active matter via Langevin dynamics simulations. We pay attention to the effect of rotational friction coefficient and rotational noise on the phase behavior. A homogeneous system without rotational noise exhibits a sharp discontinuous transition of orientational order from an isotropic to a polar state with the increase of rotational friction coefficient. When there is rotational noise, the transition becomes continuous. The formation of polar state originates from the effective alignment effect due to the mutual coupling of the positional and orientational degrees of freedom of each disk. The rotational noise could weaken the alignment effect and cause the large spatial density inhomogeneity, while the translational noise homogenizes the system. Our model makes further conceptual progress on how the microscopic interaction among self-driven agents yields effective alignment.
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- 2023
12. Facile metal-free synthesis of pyrrolo[3,2-b]pyrrolyl-based conjugated microporous polymers for high-performance photocatalytic degradation of organic pollutants
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Jing Han Wang, Chih-Ling Chang, Zhe Wei Zhang, and Ahmed F. M. EL-Mahdy
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Polymers and Plastics ,Organic Chemistry ,Bioengineering ,Biochemistry - Abstract
An efficient and metal-free approach to the synthesis of new kinds of CMPs (pyrrolo[3,2-b]pyrrolyl-based CMPs) on a gram scale within a short time has been developed for remarkable adsorbent and photocatalytic degradation of organic pollutants.
- Published
- 2022
13. An injectable, dual crosslinkable hybrid pectin methacrylate (PECMA)/gelatin methacryloyl (GelMA) hydrogel for skin hemostasis applications
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Jing-Han Wang, Yi-Chen Ethan Li, Rúben Pereira, Ru-Sin Lin, Nian-Yun Tsai, Ching-Wen Tsai, and Chao-Ying Chiang
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food.ingredient ,Swine ,macromolecular substances ,02 engineering and technology ,Absorption (skin) ,Methacrylate ,Biochemistry ,Gelatin ,Cell Line ,Injections ,Physical Phenomena ,Mice ,03 medical and health sciences ,food ,Structural Biology ,medicine ,Animals ,Interpenetrating polymer network ,Molecular Biology ,030304 developmental biology ,chemistry.chemical_classification ,Hemostasis ,Wound Healing ,0303 health sciences ,Chemistry ,technology, industry, and agriculture ,General Medicine ,Polymer ,021001 nanoscience & nanotechnology ,Self-healing hydrogels ,Methacrylates ,Pectins ,Calcium ,Swelling ,medicine.symptom ,0210 nano-technology ,Porosity ,Biomedical engineering - Abstract
Biomaterials for effective hemorrhage control are urgently needed in clinics as uncontrolled bleeding is associated with high mortality. Herein, we developed an injectable and in situ photo-crosslinkable hybrid hemostatic hydrogel by combining pectin methacrylate (PECMA) and gelatin methacryloyl (GelMA). This modular material system combines ionic- and photo-crosslinking chemistries to design interpenetrating networks (IPN) exhibiting tunable rheology, highly porous structure, and controllable swelling and mechanical properties. By simply changing the calcium (0–15 mM) and polymer (1.5–7%) content used for the sequential crosslinking of hydrogels via calcium gelation and UV-photopolymerization, it was possible to precisely modulate the injectability, degradation, and swelling ratio. Moreover, it is demonstrated that PECMA/GelMA hydrogels present good cytocompatibility and uniquely synergize the hemostatic properties of calcium ions on PECMA, the amine residues on GelMA, and the highly porous network toward rapid blood absorption and fast coagulation effect. An in vitro porcine skin bleeding model confirmed that the hydrogel could be directly injected into the wound and rapidly photo-crosslinked, circumventing the bleeding and decreasing the coagulation time by 39%. Importantly, the crosslinked hydrogel could be easily removed to prevent secondary wound injury. Overall, this injectable hybrid PECMA/GelMA hydrogel stands as a promising hemostatic material.
- Published
- 2021
14. eEF1A Interacts with the NS5A Protein and Inhibits the Growth of Classical Swine Fever Virus
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Su Li, Shuo Feng, Jing-Han Wang, Wen-Rui He, Hua-Yang Qin, Hong Dong, Lian-Feng Li, Shao-Xiong Yu, Yongfeng Li, and Hua-Ji Qiu
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classical swine fever virus ,virus–host interactions ,NS5A ,eukaryotic elongation factor 1A ,internal ribosome entry site ,Microbiology ,QR1-502 - Abstract
The NS5A protein of classical swine fever virus (CSFV) is involved in the RNA synthesis and viral replication. However, the NS5A-interacting cellular proteins engaged in the CSFV replication are poorly defined. Using yeast two-hybrid screen, the eukaryotic elongation factor 1A (eEF1A) was identified to be an NS5A-binding partner. The NS5A–eEF1A interaction was confirmed by coimmunoprecipitation, glutathione S-transferase (GST) pulldown and laser confocal microscopy assays. The domain I of eEF1A was shown to be critical for the NS5A–eEF1A interaction. Overexpression of eEF1A suppressed the CSFV growth markedly, and conversely, knockdown of eEF1A enhanced the CSFV replication significantly. Furthermore, eEF1A, as well as NS5A, was found to reduce the translation efficiency of the internal ribosome entry site (IRES) of CSFV in a dose-dependent manner, as demonstrated by luciferase reporter assay. Streptavidin pulldown assay revealed that eEF1A could bind to the CSFV IRES. Collectively, our results suggest that eEF1A interacts with NS5A and negatively regulates the growth of CSFV.
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- 2015
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15. Insights into aggregation dynamics of NACore peptides from coarse-grained simulations
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Rui‐jing Huang, Ran Tang, Xiang‐yan Song, Jing‐han Wang, Kang Chen, and Wen‐de Tian
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Structural Biology ,Molecular Biology ,Biochemistry - Abstract
Alpha(α)-synuclein is closely related to the pathogenesis of Parkinson's disease (PD). The NACore, a fragment of α-synuclein, is considered to be the key region of α-synuclein that causes PD. The aggregation dynamics of NACores are studied via coarse-grained molecular dynamics simulations. We find that NACores can self-assemble into a large cluster at high concentrations. The aggregation dynamics can be divided into three stages. The growth kinetics for the first and second stages follows the power law, S
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- 2022
16. Effects of environment-relevant concentrations of antibiotics on seawater Chlorella sp. biofilm in artificial mariculture effluent
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Chong Yu, Chi Li, Ying Zhang, Xiang Du, Jing-Han Wang, Zhan-You Chi, and Qian Zhang
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Agronomy and Crop Science - Published
- 2023
17. Transcriptome-wide subtyping of pediatric and adult T cell acute lymphoblastic leukemia in an international study of 707 cases
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Yu-Ting Dai, Fan Zhang, Hai Fang, Jian-Feng Li, Gang Lu, Lu Jiang, Bing Chen, Dong-Dong Mao, Yuan-Fang Liu, Jin Wang, Li-Jun Peng, Chong Feng, Hai-Feng Chen, Jun-Xi Mu, Qun-Ling Zhang, Hao Wang, Hany Ariffin, Tan Ah Moy, Jing-Han Wang, Yin-Jun Lou, Su-Ning Chen, Qian Wang, Hong Liu, Zhe Shan, Itaru Matsumura, Yasushi Miyazaki, Takahiko Yasuda, Li-Ping Dou, Xiao-Jing Yan, Jin-Song Yan, Allen Eng-Juh Yeoh, De-Pei Wu, Hitoshi Kiyoi, Fumihiko Hayakawa, Jie Jin, Sheng-Yue Wang, Xiao-Jian Sun, Jian-Qing Mi, Zhu Chen, Jin-Yan Huang, and Sai-Juan Chen
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Multidisciplinary ,Mutation ,Humans ,Child ,Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ,Transcriptome - Abstract
T cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy of T cell progenitors, known to be a heterogeneous disease in pediatric and adult patients. Here we attempted to better understand the disease at the molecular level based on the transcriptomic landscape of 707 T-ALL patients (510 pediatric, 190 adult patients, and 7 with unknown age; 599 from published cohorts and 108 newly investigated). Leveraging the information of gene expression enabled us to identify 10 subtypes (G1–G10), including the previously undescribed one characterized by GATA3 mutations, with GATA3R276Q capable of affecting lymphocyte development in zebrafish. Through associating with T cell differentiation stages, we found that high expression of LYL1/LMO2/SPI1/HOXA (G1–G6) might represent the early T cell progenitor, pro/precortical/cortical stage with a relatively high age of disease onset, and lymphoblasts with TLX3/TLX1 high expression (G7–G8) could be blocked at the cortical/postcortical stage, while those with high expression of NKX2-1/TAL1/LMO1 (G9–G10) might correspond to cortical/postcortical/mature stages of T cell development. Notably, adult patients harbored more cooperative mutations among epigenetic regulators, and genes involved in JAK-STAT and RAS signaling pathways, with 44% of patients aged 40 y or above in G1 bearing DNMT3A/IDH2 mutations usually seen in acute myeloid leukemia, suggesting the nature of mixed phenotype acute leukemia.
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- 2022
18. Whole-Transcriptome Sequencing Identifies Key Differentially Expressed mRNAs, miRNAs, lncRNAs, and circRNAs Associated with CHOL
- Author
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Li-Peng Gu, Xiaoqing Jiang, Jing-Han Wang, Yu-Shui Ma, Ting-Miao Wu, Kaijian Chu, Xiao-Hui Jiang, Zhi-Jun Wu, Gao-Ren Wang, Qingxiang Gao, Yi Shi, Da Fu, Ji-Bin Liu, Su-Qing Zhang, Hui-Min Wang, Bin Yi, and Zhi-Zhen Li
- Subjects
0301 basic medicine ,Spliceosome ,miR-144-3p ,Competing endogenous RNA ,lcsh:RM1-950 ,ceRNA ,Computational biology ,Protein degradation ,Biology ,Article ,Transcriptome ,03 medical and health sciences ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,0302 clinical medicine ,Downregulation and upregulation ,030220 oncology & carcinogenesis ,Drug Discovery ,microRNA ,RNA splicing ,Molecular Medicine ,DEG ,CHOL ,spliceosome ,Gene - Abstract
To systematically evaluate the whole-transcriptome sequencing data of cholangiocarcinoma (CHOL) to gain more insights into the transcriptomic landscape and molecular mechanism of this cancer, we performed whole-transcriptome sequencing based on the tumorous (C) and their corresponding non-tumorous adjacent to the tumors (CP) from eight CHOL patients. Subsequently, differential expression analysis was performed on the C and CP groups, followed by functional interaction prediction analysis to investigate gene-regulatory circuits in CHOL. In addition, The Cancer Genome Atlas (TCGA) for CHOL data was used to validate the results. In total, 2,895 differentially expressed messenger RNAs (dif-mRNAs), 56 differentially expressed microRNAs (dif-miRNAs), 151 differentially expressed long non-coding RNAs (dif-lncRNAs), and 110 differentially expressed circular RNAs (dif-circRNAs) were found in CHOL samples compared with controls. Enrichment analysis on those differentially expressed genes (DEGs) related to miRNA, lncRNA, and circRNA also identified the function of spliceosome. The downregulated hsa-miR-144-3p were significantly enriched in the competing endogenous RNA (ceRNA) complex network, which also included 7 upregulated and 13 downregulated circRNAs, 7 upregulated lncRNAs, and 90 upregulated and 40 downregulated mRNAs. Moreover, most of the DEGs and a few of the miRNAs (such as hsa-miR-144-3p) were successfully validated by TCGA data. The genes involved in RNA splicing and protein degradation processes and miR-144-3p may play fundamental roles in the pathogenesis of CHOL., Graphical Abstract, The whole-transcriptome sequencing data of cholangiocarcinoma (CHOL) were systematically evaluated to gain more insights into the transcriptomic landscape and molecular mechanism of this cancer.
- Published
- 2020
19. Long Noncoding RNA OIP5-AS1 Promotes the Progression of Liver Hepatocellular Carcinoma via Regulating the hsa-miR-26a-3p/EPHA2 Axis
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Yi Shi, Xiongwen Zhang, Ting-Miao Wu, Liu Li, Chang-Chun Ling, Li-Peng Gu, Jing-Han Wang, Da Fu, Zhi-Zhen Li, Xuchao Zhu, Fei Yu, Yu-Shui Ma, Kaijian Chu, Qingxiang Gao, Lin-Lin Tian, Ji-Bin Liu, Hui-Min Wang, Xiaoqing Jiang, and Bin Yi
- Subjects
0301 basic medicine ,Cell ,Biology ,Article ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Drug Discovery ,medicine ,Luciferase ,OIP5-AS1 ,Cell growth ,lcsh:RM1-950 ,medicine.disease ,EPH receptor A2 ,Long non-coding RNA ,030104 developmental biology ,medicine.anatomical_structure ,lcsh:Therapeutics. Pharmacology ,Apoptosis ,LIHC ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,hsa-miR-26a-3p ,Cancer research ,Molecular Medicine ,EPHA2 ,prognosis - Abstract
Numerous studies have suggested that dysregulated long noncoding RNAs (lncRNAs) contributed to the development and progression of many cancers. lncRNA OIP5 antisense RNA 1 (OIP5-AS1) has been reported to be increased in several cancers. However, the roles of OIP5-AS1 in liver hepatocellular carcinoma (LIHC) remain to be investigated. In this study, we demonstrated that OIP5-AS1 was upregulated in LIHC tissue specimens and its overexpression was associated with the poor survival of patients with LIHC. Furthermore, loss-of function experiments indicated that OIP5-AS1 promoted cell proliferation and inhibited cell apoptosis both in vitro and in vivo. Moreover, binding sites between OIP5-AS1 and hsa-miR-26a-3p as well as between hsa-miR-26a-3p and EPHA2 were confirmed by luciferase assays. Finally, a rescue assay was performed to prove the effect of the OIP5-AS1/hsa-miR-26a-3p/EPHA2 axis on LIHC cell biological behaviors. Based on all of the above findings, our results suggested that OIP5-AS1 promoted LIHC cell proliferation and invasion via regulating the hsa-miR-26a-3p/EPHA2 axis., Graphical Abstract, Upregulated OIP5-AS1 promoted LIHC cell proliferation and invasion via regulating the hsa-miR-26a-3p/EPHA2 axis both in vitro and in vivo.
- Published
- 2020
20. The long overlooked microalgal nitrous oxide emission: Characteristics, mechanisms, and influencing factors in microalgae-based wastewater treatment scenarios
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Ying, Zhang, Jing-Han, Wang, Jing-Tian, Zhang, Zhan-You, Chi, Fan-Tao, Kong, and Qian, Zhang
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Environmental Engineering ,Bacteria ,Nitrogen ,Nitrous Oxide ,Nitrification ,Pollution ,Carbon ,Water Purification ,Greenhouse Gases ,Bioreactors ,Ammonia ,Microalgae ,Denitrification ,Environmental Chemistry ,Waste Management and Disposal ,Nitrites - Abstract
Microalgae-based wastewater treatment is particularly advantageous in simultaneous CO
- Published
- 2023
21. Study on liquid sloshing characteristics of a swaying rectangular tank with a rolling baffle
- Author
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Jing-Han Wang and Shi-Li Sun
- Subjects
Slosh dynamics ,General Mathematics ,General Engineering ,Phase (waves) ,Baffle ,Mechanics ,01 natural sciences ,010305 fluids & plasmas ,Constant linear velocity ,Free surface ,0103 physical sciences ,Point (geometry) ,Boundary value problem ,Free surface elevation ,010301 acoustics ,Geology - Abstract
This study addresses the sloshing characteristics of a liquid contained in a tank with a vertical baffle mounted at the bottom of the tank. Liquid sloshing characteristics are studied through an analytical solution procedure based on the linear velocity potential theory. The tank is forced to sway horizontally and periodically, while the baffle is fixed to the tank or rolling around a hinged point. The rectangular tank flow field is divided into a few sub-domains. The potentials are solved by a separate variable method, and the boundary conditions and matching requirements between adjacent sub-domains are used to determine the sole solution. The free surface elevations with no baffle or a low fixed baffle are compared with those in published data, and the correctness and reliability of the present method are verified. Then the baffle is forced to rotate around the bottom-mounted point. It is found that the baffle’s motion, including the magnitude and the phase together, can be adjusted to suppress the free surface elevation, and even the sloshing wave can be almost eliminated.
- Published
- 2019
22. Donor–acceptor conjugated microporous polymers based on Thiazolo[5,4-d]thiazole building block for high-performance visible-light-induced H2 production
- Author
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Chih-Ling Chang, Ahmed M. Elewa, Jing Han Wang, Ho-Hsiu Chou, and Ahmed F.M. EL-Mahdy
- Subjects
Mechanics of Materials ,General Materials Science ,General Chemistry ,Condensed Matter Physics - Published
- 2022
23. Impact of Chemotherapy Delay on Overall Survival for AML with IDH1/2 Mutations: A Study in Adult Chinese Patients.
- Author
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Jing-Han Wang, Qi Guo, Zhi-Xin Ma, Qiu-Ling Ma, Meng-Xia Yu, Xiu-Feng Yin, Sha-Sha Lu, Hong-Qiong Xie, Yue-Hong Jiang, Dan Shen, Li-Ya Ma, Hui Shi, Wen-Juan Yu, Ye-Jiang Lou, Ying Li, Min Yang, Gai-Xiang Xu, Li-Ping Mao, Jian-Hu Li, Huan-Ping Wang, Dong-Mei Wang, Ju-Ying Wei, Hong-Yan Tong, Jian Huang, and Jie Jin
- Subjects
Medicine ,Science - Abstract
The effect of time from diagnosis to treatment (TDT) on overall survival of patients with acute myeloid leukemia (AML) remains obscure. Furthermore, whether chemotherapy delay impacts overall survival (OS) of patients with a special molecular subtype has not been investigated. Here, we enrolled 364 cases of AML to assess the effect of TDT on OS by fractional polynomial regression in the context of clinical parameters and genes of FLT3ITD, NPM1, CEBPA, DNMT3a, and IDH1/2 mutations. Results of the current study show IDH1/2 mutations are associated with older age, M0 morphology, an intermediate cytogenetic risk group, and NPM1 mutations. TDT associates with OS for AML patients in a nonlinear pattern with a J shape. Moreover, adverse effect of delayed treatment on OS was observed in patients with IDH1/2 mutations, but not in those with IDH1/2 wildtype. Therefore, initiating chemotherapy as soon as possible after diagnosis might be a potential strategy to improve OS in AML patients with IDH1/2 mutations.
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- 2015
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24. High robustness of attached Chlorella sp. on semi-continuous low strength effluent polishing under axenic and xenic conditions
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Ya-Nan Wang, Jing-Tian Zhang, Jing-Han Wang, Zhan-You Chi, and Qian Zhang
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Agronomy and Crop Science - Published
- 2022
25. Does splenectomy significantly improve the prognosis of hepatocellular carcinoma patients with hypersplenism? A systematic review and meta-analysis
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Jing-Han Wang, Xiaoqing Jiang, Jin-Kai Feng, and Xue-Bing Shi
- Subjects
medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Splenectomy ,Hazard ratio ,Retrospective cohort study ,General Medicine ,Perioperative ,Odds ratio ,Publication bias ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Internal medicine ,Concomitant ,Medicine ,Original Article ,030211 gastroenterology & hepatology ,Hepatectomy ,business - Abstract
BACKGROUND: Whether hepatocellular carcinoma (HCC) patients with hypersplenism can benefit from splenectomy is unclear. This study aimed at exploring the efficacy and safety of concurrent splenectomy for HCC patients with hypersplenism. METHODS: PubMed, EMBASE and Web of Science databases were systematically searched to compare data on the combination of hepatectomy or transhepatic arterial infusion (TAI) with splenectomy (the splenectomy group) with data on hepatectomy or TAI alone (the non-splenectomy group) for the treatment of HCC with hypersplenism. Prospective clinical trials or retrospective cohort studies from inception to May 10, 2020 were considered eligible for this analysis. The relevant outcomes, including patients’ demographics, clinicopathologic characteristics, perioperative indices and long-term outcomes, were independently extracted by two investigators. Publication bias for overall survival (OS) and disease-free survival (DFS) was qualitatively assessed by funnel plots and quantitatively evaluated by Begg’s and Egger’s tests. RESULTS: Nine retrospective studies including 1,650 patients were analyzed. Short-term outcomes suggested that the incidence rate of postoperative complications, including portal or splenic vein thrombosis [odds ratio (OR) =26.28, P
- Published
- 2021
26. The Modified Rectangle Flap Epicanthoplasty: A Novel and Individualized Design
- Author
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Yong-Qiang Guo, Xiaoqin Liang, Shengjian Tang, Chunlei Miao, Shen-Xing Tan, Jing-Han Wang, Zhe Cao, and Changying Niu
- Subjects
medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,030230 surgery ,medicine.disease ,Lacrimal apparatus ,Surgery ,Lacrimal caruncle ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Hypertrophic scar ,Epicanthoplasty ,Plastic surgery ,0302 clinical medicine ,medicine.anatomical_structure ,Otorhinolaryngology ,medicine ,Canthus ,Interpupillary distance ,business - Abstract
The epicanthal fold is ordinary in the eyelids of Asians, and the aesthetic appearance of eyelid surgery could be reduced and undermined; thus, medial epicanthoplasty is commonly performed to eliminate the effect of the epicanthal fold with less scarring. At present, there are a lot of techniques that have been described for the treatment of epicanthal fold. The potential problems, however, such as visible scar or under correction in the medial canthus area are challenges to surgeons. The purpose of our study was to explore a novel and individualized design using a modified rectangle flap with acceptable functional and aesthetic outcomes. From January 2017 to January 2018, epicanthoplasty was performed for 40 patients by using a modified rectangle flap. All patients underwent double-eyelid surgery at the same time when they needed it. The evaluation criteria included the intercanthal distance (ICD), interpupillary distance (IPD), the ratio of ICD to IPD (ICD ratio), scar visibility, and cosmetic results. From January 2017 to January 2018, the modified rectangle flap method was carried out on 40 patients, who were evaluated at follow-up from 7 to 15 months. The average intercanthal length was 36.9 ± 2.2 mm preoperatively and decreased significantly to 31.5 ± 1.8 mm postoperatively, 7 months after the surgery (P
- Published
- 2019
27. Metallocycle‐Supported POM‐Based Metal‐Organic Frameworks Assembled from Isomeric N‐Rich Ligands: Structures and Selective Adsorption and Separation of Cationic Dyes
- Author
-
Haijun Wang, Hai-Yan Zhang, Jing-Han Wang, Song Chen, Lei Liu, and Jingwen Sun
- Subjects
Inorganic Chemistry ,Crystal ,Chemistry ,Selective adsorption ,Polymer chemistry ,Cationic polymerization ,Metal-organic framework ,Metallacycle - Published
- 2019
28. A novel prognostic scoring model for newly diagnosed FLT3-ITD-positive acute myeloid leukemia
- Author
-
Yi, Zhang, Bi-De, Zhao, Cheng-Cheng, Wang, Yun-Gui, Wang, Hua-Feng, Wang, Jing-Han, Wang, Li-Xia, Liu, Feng, Lou, Shan-Bo, Cao, Xiao-Xia, Hu, Ai-Jie, Huang, Jian-Min, Yang, Hai-Tao, Meng, Wen-Juan, Yu, Hong-Yan, Tong, Jian-Min, Wang, and Jie, Jin
- Subjects
body regions ,Original Article ,psychological phenomena and processes - Abstract
FMS-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) is one of the most common somatic mutations in acute myeloid leukemia (AML). However, the molecular structure characteristics and widely accepted prognostic factors for FLT3-ITD are still not well described. This study aimed to retrospectively examine 81 patients with FLT3-ITD-positive AML diagnosed and treated at the First Affiliated Hospital of Zhejiang University from December 2013 to March 2018 using the next-generation sequencing 185-gene platform. High variant allele frequency (VAF) [> 0.48, P = 0.0089 for overall survival (OS), P = 0.13 for relapse-free survival (RFS)], multiple ITDs (> 1 ITDs, P = 0.011 for OS, P = 0.033 for RFS) and longer insertion length (> 69 bp, P = 0.14 for OS, P = 0.0078 for RFS) predicted poor survival. The study further proposed an easily applicable scoring model for OS using the Least Absolute Shrinkage and Selector Operation (LASSO) Cox regression model. Also, an independent cohort of 30 patients was used for external model validation. The mode was expressed as follows: 0.659 × FLT3-ITD VAF + 0.375 × FLT3-ITD number + 0.807 × Age + 0.688 × DNMT3A + 1.939 × U2AF1 (FLT3-ITD VAF > 0.48 scored 1; FLT3-ITD number scored 1 if carried 1 ITD, 2 if carried ≥ 2 ITDs; age > 44 years scored 1, the presence of DNMT3A or U2AF1 scored 1; 0 for other conditions). It categorized patients into low-risk (L-R, score < 1, n = 20) and high-risk (H-R, score ≥ 1, n = 61) groups based on the risk score with a significant difference in survival (3-year OS, P < 0.0001; 3-year RFS, P = 0.0005). A prognostic nomogram that integrated these five factors was developed with a concordance index calculation [OS: 0.68, 95% CI (0.64-0.72)].
- Published
- 2020
29. Growth and nutrients removal characteristics of attached Chlorella sp. using synthetic municipal secondary effluent with varied hydraulic retention times and biomass harvest intervals
- Author
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Ya-Nan Wang, Hao Pang, Chong Yu, Chi Li, Jing-Han Wang, Zhan-You Chi, Yong-Ping Xu, Shu-Ying Li, Qian Zhang, and Jian Che
- Subjects
Agronomy and Crop Science - Published
- 2022
30. Prognosis for intrahepatic cholangiocarcinoma patients treated with postoperative adjuvant transcatheter hepatic artery chemoembolization
- Author
-
Kai-Jian Chu, Yi Shi, Yu-Shui Ma, Gao-Ren Wang, Zhi-Jun Wu, Jing-Han Wang, Hui-Min Wang, Chang-Chun Ling, Da Fu, Xiao-Qing Jiang, Ting-Miao Wu, Zhi-Zhen Li, and Ji-Bin Liu
- Subjects
0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,medicine.medical_treatment ,Gastroenterology ,Cholangiocarcinoma ,03 medical and health sciences ,0302 clinical medicine ,Hepatic Artery ,Internal medicine ,medicine ,Humans ,Infusions, Intra-Arterial ,Chemoembolization, Therapeutic ,Intrahepatic Cholangiocarcinoma ,business.industry ,Hazard ratio ,Publication bias ,Odds ratio ,Prognosis ,Confidence interval ,Clinical trial ,030104 developmental biology ,Oncology ,Bile Duct Neoplasms ,Chemotherapy, Adjuvant ,030220 oncology & carcinogenesis ,Meta-analysis ,business ,Adjuvant - Abstract
Objective: We used meta-analysis to evaluate the efficacy of transcatheter hepatic arterial chemoembolization (TACE) for the treatment of intrahepatic cholangiocarcinoma (ICC). Methods: We performed the meta-analysis using the R 3.12 software and the quality evaluation of data using the Newcastle-Ottawa Scale. The main outcomes were recorded as 1-year overall survival (OS), 3-year OS, 5-year OS, and hazard ratio (HR) of TACE treatment or non-TACE treatment. The heterogeneity test was performed using the Q-test based on chi-square and I2 statistics. Egger's test was used to test the publication bias. The odds ratio or HR and 95% confidence interval (CI) were used to represent the effect index. Results: Nine controlled clinical trials involving 1724 participants were included in this study; patients came mainly from China, Italy, South Korea, and Germany. In the OS meta-analysis, the 1-year and 3-year OS showed significant heterogeneity, but not the 5-year OS. TACE increased the 1-year OS (odds ratio = 2.66, 95% CI: 1.10-6.46) of the patients with ICC, but the 3- and 5-year OS rates were not significantly increased. The results had no publication bias, but the stability was weak. The HR had significant heterogeneity (I2 = 0%, P= 0.54). TACE significantly decreased the HR of ICC patients (HR = 0.59, 95% CI: 0.48-0.73). The results had no publication bias, and the stability was good. Conclusions: Treatment with TACE is effective for patients with ICC. Regular updating and further research and analysis still need to be carried out.
- Published
- 2019
31. Keggin type of polyoxometalate templated assembly of a silver-organic framework with silver-organic cages and helical structures
- Author
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Jingwen Sun, Hai-Yan Zhang, Hai-Jun Wang, Lei Liu, Song Chen, and Jing-Han Wang
- Subjects
Inorganic Chemistry ,Crystallography ,Nanocages ,010405 organic chemistry ,Chemistry ,Polyoxometalate ,Materials Chemistry ,Physical and Theoretical Chemistry ,010402 general chemistry ,Photocatalytic degradation ,01 natural sciences ,Hydrothermal circulation ,0104 chemical sciences - Abstract
A new POM-templated metal-organic framework [Ag8L4][PMo12O40]·OH {HL = 5-[4-(1,2,4-triazol-4-yl)phenyl]-1H-tetrazole} containing metal-organic nanocage and left- and right-handed helixes has been isolated under hydrothermal conditions. The Keggin POM plays a template role in the formation of macrocycle and metal-organic nanocage, as shown by X-ray diffraction analysis. The new compound represents a scarce example of POM-encapsulated metal-organic nanocage. Additionally, its photocatalytic degradation of RhB was also investigated.
- Published
- 2018
32. Change of lipids in whelks (Neptunea arthritica cumingi Crosse and Neverita didyma) during cold storage
- Author
-
Yu-Xin Liu, Kai-Qi Gang, Beiwei Zhu, Jing-Han Wang, Da-Yong Zhou, Man-Man Yu, and Chuan Li
- Subjects
030309 nutrition & dietetics ,Thiobarbituric acid ,Cold storage ,03 medical and health sciences ,Lipoxygenase ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Lipid oxidation ,TBARS ,Food science ,chemistry.chemical_classification ,0303 health sciences ,biology ,Chemistry ,Hydrolysis ,Fatty acid ,04 agricultural and veterinary sciences ,Lipase ,040401 food science ,Monoacylglycerol lipase ,Seafood ,biology.protein ,Phosphatidylcholines ,lipids (amino acids, peptides, and proteins) ,Oxidation-Reduction ,Food Science ,Polyunsaturated fatty acid - Abstract
The objective of this study was to evaluate the change of lipids in two whelk samples during cold storage. Results showed that the peroxide value (PV) and thiobarbituric acid reactive substance (TBARS) increased while the percentage of polyunsaturated fatty acid decreased, indicating that lipid oxidation occurred. The cold storage significantly reduced the levels of triacylglycerol (TAG), polar lipid (PoL), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) but increased the levels of acid value (AV), free fatty acid (FFA) and monoacylglycerol, suggesting the hydrolysis of lipids. Moreover, the results showed that the lipoxygenase, acid lipase and phospholipase contributed to the hydrolysis and oxidation of lipids in the two whelks. Additionally, partial least squares discriminant analysis showed PC was positively correlated with PE, PoL and TAG, but negatively correlated with AV, FFA, PV and TBARS, indicating there is a close relationship between hydrolysis and oxidation of lipids.
- Published
- 2019
33. The Modified Rectangle Flap Epicanthoplasty: A Novel and Individualized Design
- Author
-
Zhe, Cao, Yong-Qiang, Guo, Shen-Xing, Tan, Chang-Ying, Niu, Jing-Han, Wang, Chun-Lei, Miao, Sheng-Jian, Tang, and Xiao-Qin, Liang
- Subjects
Blepharoplasty ,Cohort Studies ,Treatment Outcome ,Asian People ,Eyelids ,Humans ,Retrospective Studies - Abstract
The epicanthal fold is ordinary in the eyelids of Asians, and the aesthetic appearance of eyelid surgery could be reduced and undermined; thus, medial epicanthoplasty is commonly performed to eliminate the effect of the epicanthal fold with less scarring. At present, there are a lot of techniques that have been described for the treatment of epicanthal fold. The potential problems, however, such as visible scar or under correction in the medial canthus area are challenges to surgeons. The purpose of our study was to explore a novel and individualized design using a modified rectangle flap with acceptable functional and aesthetic outcomes.From January 2017 to January 2018, epicanthoplasty was performed for 40 patients by using a modified rectangle flap. All patients underwent double-eyelid surgery at the same time when they needed it. The evaluation criteria included the intercanthal distance (ICD), interpupillary distance (IPD), the ratio of ICD to IPD (ICD ratio), scar visibility, and cosmetic results.From January 2017 to January 2018, the modified rectangle flap method was carried out on 40 patients, who were evaluated at follow-up from 7 to 15 months. The average intercanthal length was 36.9 ± 2.2 mm preoperatively and decreased significantly to 31.5 ± 1.8 mm postoperatively, 7 months after the surgery (P 0.01). The excellent cosmetic results, in terms of an open medial canthus, were observed during follow-up periods, with no definite recurrence, hypertrophic scar, or injury of the lacrimal apparatus. The inner canthus and lacrimal caruncle are fully exposed with an invisible scar. Both the patients and the surgeon judged that the aesthetic outcomes were excellent or good.This modified rectangular flap is an effective and personalized method of correcting the medial folds that leave no additional scar in the medial canthal area, and the procedure meets the patient's aesthetic expectations.This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
- Published
- 2019
34. Percutaneous biliary stenting combined with radiotherapy as a treatment for unresectable hilar cholangiocarcinoma
- Author
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Nian‑Xin Xia, Yong Tan, Jing‑Han Wang, Bao‑An Qiu, and Jian‑Yong Zhu
- Subjects
Cancer Research ,medicine.medical_specialty ,Percutaneous ,business.industry ,Mortality rate ,medicine.medical_treatment ,Cancer ,Articles ,Klatskin's tumor ,Biliary Stenting ,equipment and supplies ,medicine.disease ,Surgery ,Radiation therapy ,Drainage rate ,surgical procedures, operative ,Oncology ,medicine ,In patient ,Radiology ,business - Abstract
Hilar cholangiocarcinoma is often unresectable at the time of the initial diagnosis, and the provision of a definite palliative benefit is important in patients with unresectable hilar cholangiocarcinoma. The aim of the present study was to evaluate the safety of percutaneous biliary stenting and to analyze whether percutaneous biliary stenting combined with radiotherapy (RT) prolonged the stent patency and survival time of patients. In total, the cases of 38 patients with unresectable hilar cholangiocarcinoma that underwent percutaneous biliary stenting at the Navy General Hospital were retrospectively reviewed in the present study. Uncovered metallic stenting (UMS) combined with RT was administered to 25 patients, and UMS alone was administered to 13 patients. The records of early complications subsequent to percutaneous biliary stenting were collected, and the stent patency and survival times of patients were analyzed and compared between the two groups. The technical success rate of the procedure was 100% and the successful drainage rate was 86.8%. The overall early complication rate was 15.8% and the procedure-associated mortality rate was 2.6%. The median stent patency was 326 days in the UMS+RT group and 196 days in the UMS group (P=0.022). The UMS+RT group (median, 367 days) demonstrated a longer survival time compared with the UMS group (median, 267 days; P=0.025). Percutaneous biliary stenting offers a safe and effective method for the palliative treatment of patients with unresectable hilar cholangiocarcinoma, and percutaneous biliary stenting combined with RT may prolong stent patency and patient survival time.
- Published
- 2015
35. Favorable Areas Prediction in Jixi Basin
- Author
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Xun Liu, Yu Ting Zhou, Xue Rui Ma, Jing Han Wang, and Liu Wang
- Subjects
Petroleum engineering ,Coalbed methane ,Lithology ,business.industry ,Oil drilling ,Metamorphic rock ,Geochemistry ,Magnetic dip ,Coal ,General Medicine ,Structural basin ,business ,Geology - Abstract
Based on coalbed methane geology theory, make use of coal and oil drilling data, from both of the coal reservoir characteristics and conservation conditions, Through comparative analysis of a number of geological factors of coalbed thickness, burial depth, metamorphic grade, gas content, roof and floor lithology, dip angle, etc, and found that there are many similarities between Jixi Basin and Black Warrior Basin in the United States of coalbed methane reservoir conditions. Furthermore, referred the domestic and abroad coalbed methane mining experience, a number of geological factors of Jixi Basin were made grading evaluation, optimized favorable blocks, and we found that the favorable coalbed methane target area are the F and H in the south of the basin, and the F block can be used as the preferred favorable coal exploration area.
- Published
- 2015
36. [Therapeutic Efficacy of VICP+L-ASP/TKI on Adult Patients with B-ALL]
- Author
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Ming-Fang, Shen, Ju-Ying, Wei, Wen-Juan, Yu, Jing-Han, Wang, Hong-Yan, Tong, Hai-Tao, Meng, Wen-Yuan, Mai, Wen-Bin, Qian, and Jie, Jin
- Subjects
Adult ,China ,Leukocyte Count ,Neoplasm, Residual ,Risk Factors ,Antineoplastic Combined Chemotherapy Protocols ,Remission Induction ,Hematopoietic Stem Cell Transplantation ,Humans ,Induction Chemotherapy ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Prognosis ,Disease-Free Survival - Abstract
To evaluate the therapeutic efficacy of VICP+L-ASP/TKI on adult patients with B-ALL and to explore the influence factors.Forty-one adult B-ALL patients treated with VICP+L-ASP/TKI from August 2008 to June 2014 were following-up. The complete remission(CR) rate, toxicity, overall survival(OS) and event free survival(EFS) after induction treatment were analyzed, the therapeutic outcome of patients between different risk stratification subgroups was compared, the influence of standardized consolidatory and maintaining treatment as well as allogeneic hematopoietic stem cell transplantation(allo-HSCT) on survival time was analyzed.The early death not occurred in 41 patients with B-ALL including 37 cases with CR; the CR rate of 1 course treatment was 90.2%. The follow-up time lasted to March 17, 2015, the median follow-up time was 25(9-79) months; the 1 year OS rate was 75.3%, the EFS rate was 58.3%. Analysis of risk factors showed that the initial WBC count over 30×10Induction chemotherapy based on VICP+L-ASP/TKI and standardized consolidatory after remission according to the "2012 China adult acute lymphoblastic leukemia diagnosis and treatment expert consensus" can improve the therapeutic efficacy. The allo-HSCT should be actively performed for B-ALL paients with high risk(elevated initial WBC count and LDH level); at some time, the regularly monitoring MRD and adjusting therapeutic protocol according to monitoring result can promote the prognosis of adult B-ALL patients.
- Published
- 2016
37. Rapid Diagnosis and Prognosis of de novo Acute Myeloid Leukemia by Serum Metabonomic Analysis
- Author
-
Sai-Juan Chen, Jie Jin, Yihuang Wang, Wenlian Chen, Huiru Tang, Yungui Wang, He Huang, Ning Li, Yang Li, Junmin Li, Wei-Na Zhang, Jian-Qing Mi, Song-Fang Wu, Jing-Han Wang, Zhu Chen, and Limin Zhang
- Subjects
Adult ,Male ,Adolescent ,Metabolite ,Disease ,Biology ,Biochemistry ,Pathogenesis ,Young Adult ,chemistry.chemical_compound ,hemic and lymphatic diseases ,Biomarkers, Tumor ,medicine ,Humans ,Metabolomics ,Choline ,Glycolysis ,Aged ,Aged, 80 and over ,Myeloid leukemia ,General Chemistry ,Middle Aged ,Prognosis ,medicine.disease ,Leukemia, Myeloid, Acute ,Metabolic pathway ,Leukemia ,chemistry ,Case-Control Studies ,Multivariate Analysis ,Immunology ,Metabolome ,Cancer research ,Female ,Metabolic Networks and Pathways - Abstract
Acute myeloid leukemia (AML) is a life-threatening hematological disease. Novel diagnostic and prognostic markers will be essential for new therapeutics and for significantly improving the disease prognosis. To characterize the metabolic features associated with AML and search for potential diagnostic and prognostic methods, here we analyzed the phenotypic characteristics of serum metabolite composition (metabonome) in a cohort of 183 patients with de novo acute myeloid leukemia together with 232 age- and gender-matched healthy controls using (1)H NMR spectroscopy in conjunction with multivariate data analysis. We observed significant serum metabonomic differences between AML patients and healthy controls and between AML patients with favorable and intermediate cytogenetic risks. Such differences were highlighted by systems differentiations in multiple metabolic pathways including glycolysis/gluconeogenesis, TCA cycle, biosynthesis of proteins and lipoproteins, and metabolism of fatty acids and cell membrane components, especially choline and its phosphorylated derivatives. This demonstrated the NMR-based metabonomics as a rapid and less invasive method for potential AML diagnosis and prognosis. The serum metabolic phenotypes observed here indicated that integration of metabonomics with other techniques will be useful for better understanding the biochemistry of pathogenesis and progression of leukemia.
- Published
- 2013
38. Potential of Mixotrophic Cultivation of Chlorella sorokiniana for Biodiesel Production
- Author
-
Jing Han Wang, Feng Wang, and Hai Zhen Yang
- Subjects
Biodiesel ,Chlorella sorokiniana ,Productivity (ecology) ,Chemistry ,Biofuel ,Biodiesel production ,General Engineering ,Heterotroph ,Biomass ,Food science ,Mixotroph - Abstract
Biodiesel from microalgae provides a promising alternative for biofuel production. Microalgal biodiesel can be produced under three major cultivation modes, namely, photoautotrophic, heterotrophic, and mixotrophic cultivation. Studies of biodiesel production from microalgae have been reported mostly focusing on photoautotrophic cultivation, mixotrophic cultivation has rarely been researched. This paper compared the biomass productivity, lipid content, and lipid productivity ofChlorella sorokinianaunder photoautotrophic, heterotrophic, and mixotrophic cultivation. Glucose was adopted as organic carbon source at five concentrations (0.1, 0.5, 1.0, 2.0, 5.0% glucose w/v). Results displayed that microalgal growth was significantly improved in glucose supplied cultures. Synergetic effect of photoautotrophy and heterotrophy existed in mixotrophic cultivation except for 5.0% culture. Highest biomass productivity of 1.178 g·L-1·d-1and highest lipid productivity of 582 mg·L-1·d-1was observed under mixotrophic cultivation with 2.0% (w/v) glucose addition. Lipid content ofC. sorokinianawas mostly higher in stationary phase than in exponential phase. Highest lipid content of 49.37% was observed in 2.0% mixotrophic culture, followed by 47.09% in 2.0% heterotrophic culture.
- Published
- 2013
39. Mixotrophic Cultivation of Scenedesmus sp. as Biodiesel Feedstock
- Author
-
Hai Zhen Yang, Jing Han Wang, and Feng Wang
- Subjects
Biodiesel ,biology ,Productivity (ecology) ,Chemistry ,Biodiesel production ,General Engineering ,Heterotroph ,Biomass ,Food science ,Raw material ,biology.organism_classification ,Scenedesmus ,Mixotroph - Abstract
Microalgae are a promising feedstock for biodiesel production. Microalgal biodiesel can be obtained under three major cultivation modes, namely, photoautotrophic, heterotrophic, and mixotrophic cultivation. Reported studies of microalgal biodiesel production are mainly based on photoautotrophic cultivation, mixotrophic cultivation has rarely been researched. This paper compared the biomass productivity, lipid content, and lipid productivity of Scenedesmus sp. under photoautotrophic, heterotrophic, and mixotrophic cultivation. Glucose was added as organic carbon source at five concentrations (0.1, 0.5, 1.0, 2.0, 5.0% glucose w/v). Results displayed that microalgal growth was significantly improved in glucose supplied cultures. Synergetic effect of photoautotrophy and heterotrophy existed in all mixotrophic cultures. Highest biomass productivity of 1.307 g·L-1·d-1 and highest lipid productivity of 316 mg·L-1·d-1 was respectively observed under mixotrophic cultivation with 5.0% and 1.0% (w/v) glucose addition. Lipid content of Scenedesmus sp. under mixotrophic cultivation was mostly higher in stationary phase than in exponential phase. Highest lipid content of 27.73% was observed in 1.0% mixotrophic culture, followed by 24.66% in 1.0% heterotrophic culture.
- Published
- 2013
40. Oncometabolite d-2-hydroxyglutarate impairs α-ketoglutarate dehydrogenase and contractile function in rodent heart
- Author
-
Heidi Vitrac, Xiaotian Zhang, Margaret A. Goodell, Heinrich Taegtmeyer, Jing Han Wang, Anja Karlstaedt, Hernan G. Vasquez, and Romain Harmancey
- Subjects
0301 basic medicine ,ATP citrate lyase ,030204 cardiovascular system & hematology ,IDH2 ,Methylation ,Glutarates ,Histones ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Histone methylation ,Animals ,Humans ,Ketoglutarate Dehydrogenase Complex ,Epigenetics ,Oxidative decarboxylation ,Multidisciplinary ,biology ,Myocardium ,Acetylation ,Biological Sciences ,Isocitrate Dehydrogenase ,Cell biology ,Citric acid cycle ,030104 developmental biology ,Histone ,Isocitrate dehydrogenase ,Biochemistry ,Hematologic Neoplasms ,Mutation ,biology.protein ,ATP Citrate (pro-S)-Lyase ,Cardiomyopathies - Abstract
Hematologic malignancies are frequently associated with cardiac pathologies. Mutations of isocitrate dehydrogenase 1 and 2 (IDH1/2) occur in a subset of acute myeloid leukemia patients, causing metabolic and epigenetic derangements. We have now discovered that altered metabolism in leukemic cells has a profound effect on cardiac metabolism. Combining mathematical modeling and in vivo as well as ex vivo studies, we found that increased amounts of the oncometabolite d-2-hydroxyglutarate (D2-HG), produced by IDH2 mutant leukemic cells, cause contractile dysfunction in the heart. This contractile dysfunction is associated with impaired oxidative decarboxylation of α-ketoglutarate, a redirection of Krebs cycle intermediates, and increased ATP citrate lyase (ACL) activity. Increased availability of D2-HG also leads to altered histone methylation and acetylation in the heart. We propose that D2-HG promotes cardiac dysfunction by impairing α-ketoglutarate dehydrogenase and induces histone modifications in an ACL-dependent manner. Collectively, our results highlight the impact of cancer cell metabolism on function and metabolism of the heart.
- Published
- 2016
41. Vitamin D serum level is associated with Child–Pugh score and metabolic enzyme imbalances, but not viral load in chronic hepatitis B patients
- Author
-
Jing-han Wang, Xinyan Zhao, Jidong Jia, Shu-jie Sun, Wei Wei, Henry W. Strobel, Jia Li, and Sohail Habib
- Subjects
Liver Cirrhosis ,Male ,medicine.medical_specialty ,Cirrhosis ,hydroxylase ,Observational Study ,Gastroenterology ,vitamin D deficiency ,03 medical and health sciences ,0302 clinical medicine ,Hepatitis B, Chronic ,Internal medicine ,medicine ,Vitamin D and neurology ,Humans ,chronic hepatitis B ,Vitamin D ,Hepatitis ,business.industry ,liver dysfunction ,25-hydroxyvitamin D3 ,cirrhosis ,General Medicine ,Odds ratio ,Hepatitis B ,Middle Aged ,Viral Load ,medicine.disease ,Cross-Sectional Studies ,HBeAg ,030220 oncology & carcinogenesis ,Immunology ,030211 gastroenterology & hepatology ,Female ,business ,Viral load ,Research Article - Abstract
Vitamin D deficiency is common in patients with chronic liver diseases. However, vitamin D status in persons with chronic hepatitis B virus (HBV) infection is not consistently reported. Specifically, the impact of liver dysfunction on vitamin D status has not been well addressed. We recruited a group of patients (n = 345) with chronic hepatitis B (n = 115), hepatitis B related cirrhosis (n = 115), and age- and gender-matched healthy controls (n = 115). Serum 25-hydroxyvitamin D3 [25(OH)D3], its related metabolic enzymes, intact parathyroid hormone were measured. Calcium, magnesium, and phosphorus were obtained from medical record. Serum 25(OH)D3 levels in chronic hepatitis B patients (7.83 ± 3.47 ng/mL) were significantly lower than that in healthy controls (9.76 ± 4.36 ng/mL, P
- Published
- 2016
42. Reprogramming of adipose-derived stem cells of hepatic cancer patients into pluripotent stem cells
- Author
-
Lin-fang Li, Yan Chen, Jing-han Wang, Li Yu, Xiu-mei Zhou, Yu-hua Fu, Qi-jun Qian, Tao Liu, Li Fan, and Huan Wang
- Subjects
Endothelial stem cell ,Induced stem cells ,Cancer stem cell ,Cancer research ,General Medicine ,Stem cell ,Biology ,Induced pluripotent stem cell ,Reprogramming ,Embryonic stem cell ,Adult stem cell - Published
- 2011
43. Novel Behavior of Heat of Micellization of Pluronics F68 and F88 in Aqueous Solutions
- Author
-
Hung-Wei Tsui, Li-Jen Chen, Ya-Huei Hsu, and Jing-Han Wang
- Subjects
Aqueous solution ,Ethylene oxide ,Enthalpy ,Surfaces and Interfaces ,Poloxamer ,Condensed Matter Physics ,Krafft temperature ,Micelle ,chemistry.chemical_compound ,Differential scanning calorimetry ,Chemical engineering ,chemistry ,Polymer chemistry ,Electrochemistry ,General Materials Science ,Propylene oxide ,Spectroscopy - Abstract
It is well understood that the heat of micellization for surfactants is monotonically decreased along with an increase in temperature. However, this behavior for polymeric surfactants has never been carefully examined. In this study, the heat of micellization of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymers (Pluronics F68 and F88) in water as a function of temperature is carefully examined by using a high-sensitivity differential scanning calorimeter (HSDSC). The critical micelle temperature (CMT) decreases along with an increase in the concentration of Pluronic F68 (or F88). The heat of micellization decreases along with an increase in the temperature, as expected, when the CMT is higher than 55 and 42 degrees C for Pluronics F68 and F88, respectively. It is interesting to observe that the heat of micellization increases along with the temperature while the temperature is below 55 and 42 degrees C for Pluronics F68 and F88, respectively. The enthalpy-entropy compensation phenomenon for the micellization of Pluronics F68 and F88 in connection with the hydrophobicity is discussed.
- Published
- 2008
44. A distinct glucose metabolism signature of acute myeloid leukemia with prognostic value
- Author
-
Jin-Song Yan, Wei Jia, Xiao-Jun Huang, Yue-Ying Wang, He Huang, Yan Li, Yihuang Wang, Tianlu Chen, Yong-Mei Zhu, Junmin Li, Jing-Han Wang, Aihua Zhao, Yuan-Fang Liu, Wenlian Chen, Zhu Chen, Sai-Juan Chen, Jie Jin, Xin Xu, Shuai Wang, Jian-Yong Li, Jian-Qing Mi, Bing-Shun Wang, Xiaojing Yan, and Depei Wu
- Subjects
Adult ,Male ,Myeloid ,Adolescent ,Metabolite ,Immunology ,HL-60 Cells ,Biology ,Biochemistry ,Antileukemic agent ,Transcriptome ,chemistry.chemical_compound ,Young Adult ,Predictive Value of Tests ,hemic and lymphatic diseases ,Cell Line, Tumor ,medicine ,Humans ,neoplasms ,Aged ,Aged, 80 and over ,Myeloid Neoplasia ,Myeloid leukemia ,Cell Biology ,Hematology ,U937 Cells ,Middle Aged ,medicine.disease ,Prognosis ,Metabolic pathway ,Leukemia ,Leukemia, Myeloid, Acute ,medicine.anatomical_structure ,Glucose ,HEK293 Cells ,chemistry ,Case-Control Studies ,Cancer research ,Cytarabine ,Female ,Metabolic Networks and Pathways ,medicine.drug - Abstract
Acute myeloid leukemia (AML) is a group of hematological malignancies with high heterogeneity. There is an increasing need to improve the risk stratification of AML patients, including those with normal cytogenetics, using molecular biomarkers. Here, we report a metabolomics study that identified a distinct glucose metabolism signature with 400 AML patients and 446 healthy controls. The glucose metabolism signature comprises a panel of 6 serum metabolite markers, which demonstrated prognostic value in cytogenetically normal AML patients. We generated a prognosis risk score (PRS) with 6 metabolite markers for each patient using principal component analysis. A low PRS was able to predict patients with poor survival independently of well-established markers. We further compared the gene expression patterns of AML blast cells between low and high PRS groups, which correlated well to the metabolic pathways involving the 6 metabolite markers, with enhanced glycolysis and tricarboxylic [corrected] acid cycle at gene expression level in low PRS group. In vitro results demonstrated enhanced glycolysis contributed to decreased sensitivity to antileukemic agent arabinofuranosyl cytidine (Ara-C), whereas inhibition of glycolysis suppressed AML cell proliferation and potentiated cytotoxicity of Ara-C. Our study provides strong evidence for the use of serum metabolites and metabolic pathways as novel prognostic markers and potential therapeutic targets for AML.
- Published
- 2014
45. High IDH1 expression is associated with a poor prognosis in cytogenetically normal acute myeloid leukemia
- Author
-
Qiu-Ling, Ma, Jing-Han, Wang, Yun-Gui, Wang, Chao, Hu, Qi-Tian, Mu, Meng-Xia, Yu, Lei, Wang, Dong-Mei, Wang, Min, Yang, Xiu-Feng, Yin, Fei-Fei, Chen, Sha-Sha, Lu, Jian, Chen, Zhi-Juan, Zhu, Sai-Juan, Chen, and Jie, Jin
- Subjects
Adult ,Aged, 80 and over ,Male ,Adolescent ,Gene Expression Regulation, Leukemic ,Gene Expression Profiling ,Karyotype ,Middle Aged ,Prognosis ,Survival Analysis ,Isocitrate Dehydrogenase ,Up-Regulation ,Leukemia, Myeloid, Acute ,Young Adult ,Mutation ,Humans ,Female ,Nucleophosmin ,Aged - Abstract
The prognostic value of IDH1 mutations has been systematically evaluated in acute myeloid leukemia (AML) patients recently. However, the role of IDH1 expression in AML is still under exploration. To investigate the clinical significance, we analyzed the IDH1/2 expression in 320 patients with cytogenetically normal AML (CN-AML) by quantitative real-time reverse-transcription polymerase chain reaction. High expression of IDH1 was predominant in patients with FLT3-ITD and DNMT3A mutations and less prevalent in cases with CEBPA double allele mutations. Strong association was observed between high IDH1 expression and low expression of microRNA 181 family. Prognosis was adversely affected by high IDH1 expression, with shorter overall survival and event-free survival in the context of clinical characteristics, including age, WBC count, and gene mutations of NPM1, FLT3-ITD, CEBPA, IDH1, IDH2 and DNMT3A in CN-AML. Moreover, the clinical outcome of IDH1 expression in terms of overall survival, event-free survival and complete remission rate still remained in multivariate models in CN-AML. Importantly, the prognostic value was validated using the published microarray data from 79 adult patients treated according to the German AMLCG-1999 protocol. Our results demonstrated that high IDH1 expression is associated with a poor prognosis of CN-AML.
- Published
- 2014
46. Reply to Struys: Role of biomarker of 2-hydroxyglutarate in acute myeloid leukemia
- Author
-
Wei Jia, Yang Shen, Wenlian Chen, Zhu Chen, Jing-Han Wang, and Sai-Juan Chen
- Subjects
Male ,2-Hydroxyglutarate ,China ,Multidisciplinary ,IDH1 ,Gene Expression Regulation, Leukemic ,Myeloid leukemia ,Endogeny ,Biology ,DNA Methylation ,Middle Aged ,Disease-Free Survival ,Isocitrate Dehydrogenase ,Glutarates ,Survival Rate ,Leukemia, Myeloid, Acute ,Immunology ,Mutation ,Cancer research ,Biomarker (medicine) ,Humans ,Female ,Letters - Abstract
The 2-hydroxyglutarate (2-HG) has been reported to result from mutations of isocitrate dehydrogenase 1 and 2 (IDH1 and IDH2) genes and to function as an "oncometabolite." To evaluate the clinical significance of serum 2-HG levels in hematologic malignancies, acute myeloid leukemia (AML) in particular, we analyzed this metabolite in distinct types of human leukemia and lymphoma and established the range of serum 2-HG in appropriate normal control individuals by using gas chromatograph-time-of-flight mass spectrometry. Aberrant serum 2-HG pattern was detected in the multicenter group of AML, with 62 of 367 (17%) patients having 2-HG levels above the cutoff value (2.01, log2-transformed from 4.03 μg/mL). IDH1/2 mutations occurred in 27 of 31 (87%) AML cases with very high 2-HG, but were observed only in 9 of 31 (29%) patients with moderately high 2-HG, suggesting other genetic or biochemical events may exist in causing 2-HG elevation. Indeed, glutamine-related metabolites exhibited a pattern in favor of 2-HG synthesis in the high 2-HG group. In AML patients with cytogenetically normal AML (n = 234), high 2-HG represented a negative prognostic factor in both overall survival and event-free survival. Univariate and multivariate analyses confirmed high serum 2-HG as a strong prognostic predictor independent of other clinical and molecular features. We also demonstrated distinct gene-expression/DNA methylation profiles in AML blasts with high 2-HG compared with those with normal ones, supporting a role that 2-HG plays in leukemogenesis.
- Published
- 2013
47. Impact of Chemotherapy Delay on Overall Survival for AML with I DH1/2 Mutations: A Study in Adult Chinese Patients
- Author
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Wen Juan Yu, Ying Li, Meng Xia Yu, Hui Shi, Li Ping Mao, Xiu Feng Yin, Dongmei Wang, Fan Ping Wang, Sha Sha Lu, Hong Yan Tong, Jie Jin, Qiong Hong Xie, Ye Jiang Lou, Gai Xiang Xu, Qiu Ling Ma, Jing Han Wang, Min Yang, Dan Shen, li Ya Ma, Jian Huang, Yue Hong Jiang, Ju Ying Wei, Qi Guo, Jian Hu Li, and Zhi Xin Ma
- Subjects
Oncology ,medicine.medical_specialty ,NPM1 ,Chemotherapy ,IDH1 ,business.industry ,medicine.medical_treatment ,Immunology ,Myeloid leukemia ,Context (language use) ,Cell Biology ,Hematology ,Biochemistry ,Chemotherapy regimen ,Internal medicine ,CEBPA ,medicine ,Adverse effect ,business - Abstract
The effect of time from diagnosis to treatment (TDT) on overall survival of patients with acute myeloid leukemia (AML) remain obscure. Furthermore, whether chemotherapy delay impacts overall survival (OS) of patients with a special molecular subtype has not been investigated. Here, we enrolled 364 cases of AML to assess the effect of TDT on OS by fractional polynomial regression in the context of clinical parameters and genes of FLT3 ITD, NPM1, CEBPA, DNMT3a, and IDH1/2 mutations. Results of the current study show IDH1/2 mutations are associated with older age, M0 morphology, an intermediate cytogenetic risk group, and NPM1 mutations. TDT associates with OS for AML patients in a nonlinear pattern with a J shape. Moreover, adverse effect of delayed treatment on OS was observed in patients with IDH1/2 mutations, but not in those with IDH1/2 wild type. Therefore, initiating chemotherapy as soon as possible after diagnosis might be a potential strategy to improve OS in AML patients with IDH1/2 mutations. Disclosures No relevant conflicts of interest to declare.
- Published
- 2015
48. [Establishment and characterization of a human gallbladder carcinoma cell line EH-GB1 originated from a metastatic tumor]
- Author
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Lin-fang, Li, Huan-zhang, Hu, Chen, Liu, Jing-han, Wang, Hong-ping, Wu, Hua-jun, Jin, Chang-qing, Su, Xiao-qing, Jiang, Jia, Liu, Jian-zhong, Gu, Meng-chao, Wu, and Qi-jun, Qian
- Subjects
CA-19-9 Antigen ,Abdominal Wall ,Green Fluorescent Proteins ,Mice, Nude ,Mice, SCID ,Adenocarcinoma ,Middle Aged ,Mice ,Genes, Reporter ,Abdominal Neoplasms ,Cell Line, Tumor ,Animals ,Humans ,Female ,Gallbladder Neoplasms ,Neoplasm Transplantation - Abstract
To establish a human gallbladder carcinoma cell line derived from a metastatic gallbladder carcinoma and identify its biological characteristics.Tissue samples were separated from the surgical specimen obtained from a patient with metastatic carcinoma and single-cell suspension was prepared. Then the cells were cultured in DMEM medium supplemented with 15% fetal bovine serum. The morphology of tumor cells was observed under an electron microscope. The cell growth curve was plotted. The tumorigenicity of the cell line was studied by subcutaneous inoculation in SCID mice. The cells were infected by lentiviral vector carrying fluorescent report genes (lenti-GFP and lenti-Red2) separately for expressions of GFP and Red2, respectively.A novel metastatic gallbladder carcinoma cell line was successfully established and named "EH-GB1". It could be passaged for over 20 generations with typical malignant epithelial morphology and a stable growth cycle of 24 h. Tumors were formed in all of the 10 SCID mice inoculated with EH-GB1 cells subcutaneously, and the tumor cells were tumor marker CA19-9-positive. Continuous expressions of fluorescent report genes were observed in EH-GB1 cells infected by lenti-GFP and lenti-Red2.EH-GB1 cells might be the first stable cell line of human gallbladder carcinoma established from a metastatic focus of gallbladder carcinoma. This cell line with continuous expressions of GFP and Red2 might be a novel and perfect experimental model for clinical and basic research on gallbladder carcinoma.
- Published
- 2010
49. [Study on analysis of copy paper by Fourier transform infrared spectroscopy]
- Author
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Ji-Min, Li, Yan-Ji, Wang, Jing-Han, Wang, Li-Juan, Yao, and Biao, Zhang
- Subjects
Paper ,Quality Control ,Hot Temperature ,Time Factors ,Inorganic Chemicals ,Spectroscopy, Fourier Transform Infrared ,Desiccation ,Cellulose ,Copying Processes - Abstract
A new method of fast identification of copy papers by Fourier transform infrared spectroscopy (FTIR) was developed. The kinds of filler and the cellulosic degree of crystallinity were analyzed by FTIR, and the ageing curves of cellulosic paper were studied with heating and ultraviolet light. The cellulosic degree of crystallinity was showed by the ratio of absorbance at 1 429 cm(-1) to that at 893 cm(-1), the standard deviation of different brands of copy papers was 0.010 7-0.016 0, and the standard deviation of the same brands of copy papers was 0.014 8. The kinds of filler and the cellulosic degree of crystallinity were different in copy papers from different brands of different manufacturing plants, different brands of same manufacturing plants and different manufacturing times of the same brands from the same manufacturing plants, and the curves of ageing were different with heating and ultraviolet light. The results of fast identification of copy papers by FTIR are satisfactory.
- Published
- 2009
50. [Determination of writing age of blue ballpoint pen inks by high performance liquid chromatography]
- Author
-
Xiao-Fan, Shi, Xin-Qian, Li, Ying-Jian, Xu, Jing-Han, Wang, and Yan-Ji, Wang
- Subjects
Time Factors ,Models, Chemical ,Molecular Structure ,Writing ,Forensic Sciences ,Reproducibility of Results ,Gentian Violet ,Ink ,Coloring Agents ,Chromatography, High Pressure Liquid - Abstract
As it is a frequently encountered problem in the laboratory of forensic science nowadays to distinguish whether the questioned documents, such as deeds, contracts, and receipts, written in ballpoint pen inks are true or not, and identify the writing age of them, it is very essential to establish a simple, sensitive and accurate method to examine the similarities and differences of the ballpoint pen inks and identify the writing age. The present paper introduces a technique that allows identifying the kind and the writing age of the blue ballpoint pen inks. The technique is based on using a high performance liquid chromatographic method for distinguishing the similarities and differences in dyes of blue ballpoint pen inks and determining changes in dyes of blue ballpoint pen inks developed with age, and these changes can be evaluated by the ratio of peak areas.
- Published
- 2006
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