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Abstract Aortic dissection (AD) is a life-threatening condition with a high mortality rate and without effective pharmacological therapies. Our previous study illustrated that leukocyte immunoglobulin-like receptor B4 (LILRB4) knockdown promoted the contractile phenotypic switch and apoptosis of AD cells. This study aimed to further investigate the role of LILRB4 in animal models of AD and elucidate its underlying molecular mechanisms. Animal models of AD were established using 0.1% beta-aminopropionitrile and angiotensin II and an in vitro model was developed using platelet-derived growth factor BB (PDGF-BB). The effects of LILRB4 knockdown on histopathological changes, pyroptosis, phenotype transition, extracellular matrix (ECM), and Janus kinase 2 (JAK2)/signal transducers and activators of transcription 3 (STAT3) pathways were assessed using a series of in vivo and in vitro assays. The effects of the JAK2 inhibitor AG490 on AD cell function, phenotypic transition, and ECM were explored. LILRB4 was highly expressed in AD and its knockdown increased survival rate, reduced AD incidence, and alleviated histopathological changes in the AD mouse model. Furthermore, LILRB4 knockdown promoted contractile phenotype switch, stabilized the ECM, and inhibited pyroptosis. Mechanistically, LILRB4 knockdown inhibited the JAK2/STAT3 signaling pathway. JAK2 inhibitor AG490 inhibited cell viability and migration, enhanced apoptosis, induced G0/G1 cell cycle arrest, and suppressed S-phase progression in PDGF-BB-stimulated human aortic smooth muscle cells. LILRB4 knockdown suppresses AD development by inhibiting pyroptosis and the JAK2/STAT3 signaling pathway.

Abstract Rhenium (Re) and uranium (U) are essential proxies in reconstructing past oceanic oxygenation evolution. However, their removal in continental shelf sediments, hotspots of early diagenesis, were previously treated as quantitatively unimportant sinks in the ocean. Here we examine the sedimentary reductive removal of Re and U and their coupling with organic carbon decomposition, utilizing the 224Ra/228Th disequilibria within the East China Sea shelf. We identified positive correlations between their removal fluxes and the rates of sediment oxygen consumption or organic carbon decomposition. These correlations enable an evaluation of global shelf reductive sinks that are comparable to (for Re) or higher than (~4-fold for U) previously established suboxic/anoxic sinks. These findings suggest potential imbalances in the modern budgets of Re and U, or perhaps a substantial underestimation of their sources. Our study thus highlights shelf sedimentary reductive removal as critical yet overlooked sinks for Re and U in the modern ocean.

Abstract Acute type A aortic dissection is a severe cardiovascular disease characterized by rapid onset and high mortality. Traditionally, urgent open aortic repair is performed after admission to prevent aortic rupture and death. However, when combined with malperfusion syndrome, the low perfusion of the superior mesenteric artery can further lead to intestinal necrosis, significantly impacting the surgery’s prognosis and potentially resulting in adverse consequences, bringing. This presents great significant challenges in treatment. Based on recent domestic and international research literature, this paper reviews the mechanism, current treatment approaches, and selection of surgical methods for poor organ perfusion caused by acute type A aortic dissection. The literature review findings suggest that central aortic repair can be employed for the treatment of acute type A aortic dissection with inadequate perfusion of the superior mesenteric artery. The superior mesenteric artery can be windowed and (/or) stented, followed by delayed aortic repair. Priority should be given to revascularization of the superior mesenteric artery, followed by central aortic repair. During central aortic repair, direct blood perfusion should be performed on the distal true lumen of the superior mesenteric artery, leading to resulting in favorable therapeutic outcomes. The research results indicate that even after surgical aortic repair, intestinal ischemic necrosis may still occur. In such cases, prompt laparotomy and necessary necrotic bowel resection are crucial for saving the patient’s life.

Abstract Acute type A aortic dissection is a life-threatening cardiovascular disease characterized by rapid onset and high mortality. Emergency surgery is the preferred and reliable treatment option. However, postoperative complications significantly impact patient prognosis. Hypoxemia, a common complication, poses challenges in clinical treatment, negatively affecting patient outcomes and increasing the risk of mortality. Therefore, it is crucial to study and comprehend the risk factors and treatment strategies for hypoxemia following acute type A aortic dissection to facilitate early intervention.

It is crucial to clarify the nonlinear effects of urban multidimensional characteristics on land surface temperature (LST). However, the combined consideration of the urban green space (UGS), water bodies, buildings, and socio-economic factors is limited. And the diurnal differences in their thermal effects have been less considered. In this study, central Beijing was taken as study area. Local climate zones (LCZ) were firstly applied to reveal spatiotemporal heterogeneity of LST. Then, the interpretable machine learning methods were utilized to quantitatively reveal nonlinear thermal effects of urban multidimensional characteristics, i.e., the UGS, water bodies, and building landscape features, and socio-economic features. The results indicated that built type LCZs have a higher average LST compared to natural type LCZs. And the LST of built type LCZs is simultaneously influenced by buildings’ density and height characteristics. Daytime LST is mainly affected by the landscape proportions of UGS, buildings, and trees, while nighttime LST is more influenced by socio-economic and building characteristics. The thermal effects of key factors exhibit nonlinear characteristics. Whether during the day or night, the impact of building coverage on LST is greater than that of building height, consistently exhibiting a warming effect. While, the building height and water body edge density factors both exhibited a reversal trend in their thermal impact between day and night. Our study also emphasized the importance of trees type in UGS and provided recommendations for UGS planning based on sensitivity and contribution considerations. These findings can help to regulate urban LST and promote sustainable urban development.

Land surface temperature (LST) captures fundamental information on the spatiotemporal variation of energy balance at the surface. The trade-off between spatial and temporal resolutions of remote sensing images (retrieved LSTs), however, restricts fine-scale thermal environmental investigations. In this context, a novel dual-layer composite framework (DCF) for LST downscaling coupling spatial autocorrelation and spatial heterogeneity was developed based on the two fundamental laws of geography and used to improve existing kernel-driven methods. Besides, a new non-parametric kernel-driven LST downscaling method (N-DLST) was also proposed under the DCF, in which Bayesian non-parametric general regression (BNGR) was applied to predict the high-resolution LSTs with auto-selected kernels. In the experiment of downscaling Landsat 8 LST from 300 m to 30 m over the highly heterogeneous urban area, the N-DLST method significantly outperformed the original kernel-driven methods, with the highest coefficient of determination (R2 = 0.93) and lowest root mean square error (RMSE = 0.85). Moreover, the enhanced effects of DCF in downscaling LST were demonstrated by comparing the accuracy of the disaggregation of radiometric surface temperature (DisTrad), the geographically weighted regression-based method (GWR), and random forest (RF) method before and after their improvements. Visual interpretation and quantitative assessments revealed that the DCF could improve the accuracy of DisTrad, GWR, and RF methods with an increase in R2 by approximately 0.09 and a decrease in RMSE by more than 0.4 °C. In the cases of LST downscaling over highly heterogeneous contexts and water bodies, N-DLST effectively preserved the textures and large-scale variations, yielding the most consistent spatial pattern with the reference LST. Given the simplicity of the modelling process and absence of auxiliary data, the DCF could strengthen the performance of both linear and nonlinear LST downscaling methods, while the N-DLST method could serve as an effective tool for high-resolution LST prediction.

Background: Surgery is the preferred treatment for acute Stanford type A aortic dissection (STAAD); however, due to the complexity of the procedure, cardiac ischaemia and cardiopulmonary bypass (CPB) time are longer than general heart surgery, leading to complications. In this present study, we used an integrated tetra-furcate graft for both modified aortic root and distal arch anastomoses (frozen elephant trunk technique, [FET]), and investigated postoperative outcomes associated with this technique in patients with STAAD. Methods: We included a total of 140 patients who underwent total arch replacement and FET between January 2019 and June 2022 in the present study, 41 patients who underwent the modified technique, and 99 who underwent the graft eversion technique. We subsequently analyzed the perioperative outcomes to compare the differences between the two techniques. Results: There were no statistically significant differences between the two groups in regards to the preoperative characteristics; however, the intraoperative CPB, cardiac ischaemia, and operation times of the modified technique group were significantly shorter than those of the eversion technique group (P = 0.02, P = 0.01, and P = 0.04, respectively), as were postoperative hypoxaemia, intensive care unit (ICU) stay, and ventilation times (P = 0.04, P = 0.03, and P = 0.04, respectively). Additionally, the degree of postoperative bilirubin elevation was milder in the modified technique group (P = 0.002 for direct bilirubin and P = 0.01 for indirect bilirubin). Conclusions: The modified anastomosis technique can significantly shorten CPB, cardiac ischemia, and operation times, and reduce the intraoperative FFP transfusion and postoperative hypoxemia times. This modified technique, therefore, is worth utilizing for patients with STAAD.

Abstract Background Prolonged postoperative ileus (PPOI) is a common complication after colorectal surgery that increases patient discomfort, hospital stay, and financial burden. However, predictive tools to assess the risk of PPOI in patients undergoing laparoscopic low anterior resection have not been developed. Thus, the purpose of this study was to develop a nomogram to predict PPOI after laparoscopic low anterior resection for rectal cancer. Methods A total of 548 consecutive patients who underwent laparoscopic low anterior resection for mid­low rectal cancer at a single tertiary medical center were retrospectively enrolled between January 2019 and January 2023. Univariate and multivariate logistic regression analysis was performed to analyze potential predictors of PPOI. The nomogram was constructed using the filtered variables and internally verified by bootstrap resampling. Model performance was evaluated by receiver operating characteristic curve and calibration curve, and the clinical usefulness was evaluated by the decision curve. Results Among 548 consecutive patients, 72 patients (13.1%) presented with PPOI. Multivariate logistic analysis showed that advantage age, hypoalbuminemia, high surgical difficulty, and postoperative use of opioid analgesic were independent prognostic factors for PPOI. These variables were used to construct the nomogram model to predict PPOI. Internal validation, conducted through bootstrap resampling, confirmed the great discrimination of the nomogram with an area under the curve of 0.738 (95%CI 0.736–0.741). Conclusions We created a novel nomogram for predicting PPOI after laparoscopic low anterior resection. This nomogram can assist surgeons in identifying patients at a heightened risk of PPOI.

Cr(VI) is known to be seriously toxic and carcinogenic. Hypoxia-inducible factor-1α (HIF-1α) is a crucial regulator to promote tumor development. In this study, we found that Cr(VI) significantly increased the expression of HIF-1α in A549 cells and in lung of BALB/c mice but not in HELF cells. Treatment with Lificiguat (YC-1), HIF-1α inhibitor, or CoCl2, HIF-1α inducer, could alter Cr(VI)-induced autophagy, glycolysis, and cell growth in A549 cells but not in HELF cells, validating the involvement of HIF-1α in these effects of Cr(VI) in A549 cells. Co-treatments of pcATG4B with YC-1, or siATG4B with CoCl2 demonstrated the role of HIF-1α / autophagy axis in inducing glycolysis and cell growth in A549 cells. In HELF cells, however, only autophagy but not HIF-1α played a role in inducing glycolysis. The protein level of p53 was significantly lower in A549 cells than in HELF cells. RITA, a p53 inducer, attenuated Cr(VI)-induced HIF-1α and LC3-II in A549 cells, suggesting that p53 might be the mechanism underlying the different effects of Cr(VI) on HIF-1α in A549 and HELF cells. Thus, p53-dependent HIF-1α / autophagy-mediated glycolysis plays a role in facilitating Cr(VI)-induced carcinogenesis.

Abstract Recent large language models (LLMs), such as ChatGPT, have demonstrated remarkable prediction performance for a growing array of tasks. However, their proliferation into high-stakes domains and compute-limited settings has created a burgeoning need for interpretability and efficiency. We address this need by proposing Aug-imodels, a framework for leveraging the knowledge learned by LLMs to build extremely efficient and interpretable prediction models. Aug-imodels use LLMs during fitting but not during inference, allowing complete transparency and often a speed/memory improvement of greater than 1000x for inference compared to LLMs. We explore two instantiations of Aug-imodels in natural-language processing: Aug-Linear, which augments a linear model with decoupled embeddings from an LLM and Aug-Tree, which augments a decision tree with LLM feature expansions. Across a variety of text-classification datasets, both outperform their non-augmented, interpretable counterparts. Aug-Linear can even outperform much larger models, e.g. a 6-billion parameter GPT-J model, despite having 10,000x fewer parameters and being fully transparent. We further explore Aug-imodels in a natural-language fMRI study, where they generate interesting interpretations from scientific data.

LncRNA is a major factor in the occurrence and development of many diseases. However, its mechanism in cerebral ischemia/reperfusion injury (CIRI) is yet unknown. In this study, the transcriptional level and methylation modification level of LncRNAs before and after mechanical thrombectomy were compared by high-throughput sequencing. Venn diagram, Spearman correlation analysis, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, TargetScan, and miRanda were used to analyze the experimental data. The results showed that four key LncRNAs changed at both transcription and methylation levels. Specifically, LncRNA FAR2, LINC02431, and AL357060.1 were downregulated and hypomethylated, while LncRNA FOXD2-AS1 was upregulated and hypomethylated. Moreover, positive regulation of angiogenesis, protein domain–specific binding, autophagy pathway, PPAR signaling pathway, and MAPK signaling pathway were co-enriched between LncRNAs with different expression levels and different methylation levels. Finally, a LncRNA-miRNA-mRNA network was constructed. Therefore, this study explored the potential key LncRNAs and regulatory mechanisms of CIRI.

BackgroundTotal mesorectal excision (TME), represents a key technique in radical surgery for rectal cancer. This study aimed to construct a preoperative nomogram for predicting the surgical difficulty of laparoscopic total mesorectal excision (L-TME) and to investigate whether there were potential benefits of robotic TME (R-TME) for patients with technically challenging rectal cancer.MethodsConsecutive mid­low rectal cancer patients receiving total mesorectal excision were included. A preoperative nomogram to predict the surgical difficulty of L-TME was established and validated. Patients with technically challenging rectal cancer were screened by calculating the prediction score of the nomogram. Then patients with technically challenging rectal cancer who underwent different types of surgery, R-TME or L-TME, were analyzed for comparison.ResultsA total of 533 consecutive patients with mid­low rectal cancer who underwent TME at a single tertiary medical center between January 2018 and January 2021 were retrospectively enrolled. Multivariable analysis demonstrated that mesorectal fat area, intertuberous distance, tumor size, and tumor height were independent risk factors for surgical difficulty. Subsequently, these variables were used to construct the nomogram model to predict the surgical difficulty of L-TME. The area under the receiver operating characteristic curve of the nomogram was 0.827 (95% CI 0.745 - 0.909) and 0.809 (95% CI 0.674- 0.944) in the training and validation cohort, respectively. For patients with technically challenging rectal cancer, R-TME was associated with a lower diverting ileostomy rate (p = 0.003), less estimated blood loss (p < 0.043), shorter procedure time (p = 0.009) and shorter postoperative hospital stay (p = 0.037).ConclusionIn this study, we established a preoperative nomogram to predict the surgical difficulty of L-TME. Furthermore, this study also indicated that R-TME has potential technical advantages for patients with technically challenging rectal cancer.

BackgroundAcute Stanford type A aortic dissection (STAAD) is a fatal condition requiring urgent surgical intervention. Owing to the complexity of the surgical process, various complications, such as neurological disorders, are common. In this study, we prioritized the reconstruction of aortic arch branches during surgery and investigated the association between prioritizing the branches and the postoperative outcomes of patients with STAAD.MethodsNinety-seven patients were included in the observational study and underwent total arch replacement and frozen elephant trunk technique between January 2018 and June 2021. Of these, 35 patients underwent the branch-priority technique, and 62 patients underwent the classic technique. By analyzing the perioperative outcomes, we compared the differences between the two techniques.ResultsThe branch priority group had significantly shorter cardiopulmonary bypass and ventilator times and earlier postoperative wake-up times than the classic group. Additionally, the ICU stay time was shorter, with a significant decrease in neurological complications and 24 h drainage in the branch priority group compared to the classic group.ConclusionThe branch priority technique can effectively provide better brain protection, resulting in earlier awakening of patients after surgery, reduced neurological complications, shorter ventilation time and decreased ICU hospitalization time. Therefore, it is recommended for use in aortic dissection surgeries.

After more than five decades, Moore’s Law for transistors is approaching the end of the international technology roadmap of semiconductors (ITRS). The fate of complementary metal oxide semiconductor (CMOS) architecture has become increasingly unknown. In this era, 3D transistors in the form of gate-all-around (GAA) transistors are being considered as an excellent solution to scaling down beyond the 5 nm technology node, which solves the difficulties of carrier transport in the channel region which are mainly rooted in short channel effects (SCEs). In parallel to Moore, during the last two decades, transistors with a fully depleted SOI (FDSOI) design have also been processed for low-power electronics. Among all the possible designs, there are also tunneling field-effect transistors (TFETs), which offer very low power consumption and decent electrical characteristics. This review article presents new transistor designs, along with the integration of electronics and photonics, simulation methods, and continuation of CMOS process technology to the 5 nm technology node and beyond. The content highlights the innovative methods, challenges, and difficulties in device processing and design, as well as how to apply suitable metrology techniques as a tool to find out the imperfections and lattice distortions, strain status, and composition in the device structures.

The saline, alkaline environment of arid soils is conducive to the diffusion of the metalloid arsenic (As). Desert plants in this area are of great ecological importance and practical value. However, there are few studies on the mechanism of arsenic action in desert plants. Therefore, in this study, Eremostachys moluccelloides Bunge was treated with different concentrations of As2O5 [As(V)] to analyze the physiological, biochemical, and transcriptomic changes of its roots and leaves and to explore the molecular mechanism of its response to As(Ⅴ) stress. The activities of catalase, superoxidase, peroxidase, and the contents of malondialdehyde and proline in roots and leaves first increased and then decreased under the As(Ⅴ) stress of different concentrations. The content of As was higher in roots than in leaves, and the As content was positively correlated with As(Ⅴ) stress concentration. In the differentially expressed gene analysis, the key enzymes of the oxidative stress response in roots and leaves were significantly enriched in the GO classification. In the KEGG pathway, genes related to the abscisic acid signal transduction pathway were co-enriched and up-regulated in roots and leaves. The related genes in the phenylpropanoid biosynthesis pathway were significantly enriched and down-regulated only in roots. In addition, the transcription factors NAC, HB-HD-ZIP, and NF-Y were up-regulated in roots and leaves. These results suggest that the higher the As(V) stress concentration, the more As is taken up by roots and leaves of E. molucelloides Bunge. In addition to causing greater oxidative damage, this may interfere with the production of secondary metabolites. Moreover, it may improve As(V) tolerance by regulating abscisic acid and transcription factors. The results will deepen our understanding of the molecular mechanism of As(Ⅴ) response in E. moluccelloides Bunge, lay the foundation for developing and applying desert plants, and provide new ideas for the phytoremediation of As pollution in arid areas.

Abstract Chlorella sp. TLD 6B, a microalgae growing in the Taklamakan Desert, Xinjiang of China, is a good model material for studying the physiological and environmental adaptation mechanisms of plants in their arid habitats, as its adaptation to the harsh desert environment has led to its strong resistance. However, when using real-time quantitative polymerase chain reaction (RT-qPCR) to analyze the gene expression of this algae under abiotic stress, it is essential to find the suitable endogenous reference genes so to obtain reliable results. This study assessed the expression stability of 9 endogenous reference genes of Chlorella sp. TLD 6B under four abiotic stresses (drought, salt, cold and heat). These genes were selected based on the analysis results calculated by the three algorithmic procedures of geNorm, NormFinder, and BestKeeper, which were ranked by refinder. Our research showed that 18S and GTP under drought stress, 18S and IDH under salt stress, CYP and 18S under cold stress, GTP and IDH under heat stress were the most stable endogenous reference genes. Moreover, UBC and 18S were the most suitable endogenous reference gene combinations for all samples. In contrast, GAPDH and α-TUB were the two least stable endogenous reference genes in all experimental samples. Additionally, the selected genes have been verified to be durable and reliable by detecting POD and PXG3 genes using above endogenous reference genes. The identification of reliable endogenous reference genes guarantees more accurate RT-qPCR quantification for Chlorella sp. TLD 6B, facilitating functional genomics studies of deserts Chlorella as well as the mining of resistance genes.

Local landscape patterns in cities can substantially alter surrounding land surface temperature (LST) and affect the surface urban heat island effect. Previous studies have confirmed that some urban functional areas produce pronounced local heat/cold island effects in cities; however, the mitigating of this effect from the perspective of surrounding landscapes has not been investigated in depth. Additionally, different types of industrial plants have not been individually studied across multiple cities as major contributors to the local heat island effect. Therefore, based on 17 mega plants in various cities in the Northern Hemisphere, this study explores the impact of surrounding landscapes on the surface industrial heat island (SIHI) effect, proposes cooling metrics from a new landscape patch perspective, and quantifies the impact of different patch configurations on the SIHI using Extreme Gradient Boosting regression models and Shapley Additive exPlanations. The primary results are as follows: 1) Regarding to the footprint of the SIHI effect, the coverage of impervious surfaces dominates the ambient LST pattern. Industrial plant types and latitudes are moderately influencing factors. 2) In terms of the cooling effect, landscape patch size and width relative to industrial plant size has a pronounced impact on the LST cooling speed. Besides, various land cover types have distinct relative cooling thresholds for patch area and distance from the plants. The influence of patch attributes on LST cooling speed is determined by the distinct land cover type. 3) What is prominent is that the patches of water bodies with larger relative footprints often exhibit higher cooling speeds. This study aims to provide guidelines for urban planners in assessing the local thermal environment and mitigating further urban warming.

Superior vena cava (SVC) stenosis is rarely caused by iatrogenic trauma. Herein, the case of a 5-year-old boy who underwent radiofrequency ablation for paroxysmal supraventricular tachycardia but developed SVC stenosis and related syndromes is reported. Notably, the child exhibited an enlarged left atrial appendage that had partially breached the pericardium. Subsequent interventions involved successful removal of the stenosis, artificial vascular reconstruction, and comprehensive radiofrequency ablation of the entire right atrium, along with ligation of the left atrial appendage under direct vision. As a result, the child experienced relief from symptoms.

BackgroundAortic dissection (AD) is a severe cardiovascular disease characterized by aortic rupture, aortic valve insufficiency, aortic branch lumen stenosis, and occlusion. Acute ST-segment elevation myocardial infarction may be the primary manifestation when aortic dissection affects the coronary artery, leading to delayed or missed diagnosis of aortic dissection, and preventing patients from receiving timely and comprehensive treatment. Simultaneous aortic repair and coronary artery bypass grafting surgery are controversial because of their high mortality rates. Personalized and optimal treatment plans for patients should be taken seriously based on their different conditions and treatment options.Case presentationA 42-year-old man who experienced 1 h of persistent precordialgia was admitted to a local second-level hospital for emergency treatment. Electrocardiogram (ECG) showed evidence of ST-segment elevation, and myocardial enzyme levels were CK-MB 18.35 ng/ml and troponin 0.42 ng/ml. The patient was treated for acute myocardial infarction (AMI) and urgently sent to the interventional catheter room. Coronary angiography showed stenosis of the starting part of the right coronary artery trunk. Thus, stent implantation was performed, and the stenosis section recovered patency; however, postoperative precordialgia was not alleviated. Computed tomography angiography (CTA) revealed a type A AD. The patient was immediately transferred to a higher-level hospital, underwent emergency surgery with cardiopulmonary bypass (CPB) ascending aorta replacement, SUN's procedure (total arch replacement and stented elephant trunk implantation), and simultaneous implantation of extracorporeal membrane oxygenation (ECMO), and regained consciousness within intensive care unit care. ECMO was discontinued when hemodynamics stabilized. The patient ultimately recovered well and was discharged.ConclusionThis case demonstrated that precordialgia is not limited to myocardial infarction but may also be accompanied by aortic dissection. Percutaneous coronary intervention (PCI) can timely and effectively restore coronary artery perfusion, strive for the opportunity of aortic repair surgery, and can overcome pump failure caused by myocardial infarction, cardiopulmonary bypass, heart block time, and myocardial ischemia-reperfusion injury. Personalized treatment is crucial for patients with complex type A aortic dissection.

IntroductionPancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignancies with poor response to immune checkpoint inhibitors. The mechanism of such poor response is not completely understood.MethodsWe assessed T-cell receptor (TCR) repertoire and RNA expression at the single-cell level using high-dimensional sequencing of peripheral blood immune cells isolated from PDAC patients and from healthy human controls. We validated RNA-sequencing data by performing mass cytometry (CyTOF) and by measuring serum levels of multiple immune checkpoint proteins.ResultsWe found that proportions of T cells (CD45+CD3+) were decreased in PDAC patients compared to healthy controls, while proportion of myeloid cells was increased. The proportion of cytotoxic CD8+ T cells and the level of cytotoxicity per cell were increased in PDAC patients, with reduced TCR clonal diversity. We also found a significantly enriched S100A9+ monocyte population and an increased level of TIM-3 expression in immune cells of peripheral blood in PDAC patients. In addition, the serum level of soluble TIM-3 (sTIM-3) was significantly higher in PDAC patients compared to the non-PDAC participants and correlated with worse survival in two independent PDAC cohorts. Moreover, sTIM-3 exhibited a valuable role in diagnosis of PDAC, with sensitivity and specificity of about 80% in the training and validation groups, respectively. We further established an integrated model by combining sTIM-3 and carbohydrate antigen 19- 9 (CA19-9), which had an area under the curve of 0.974 and 0.992 in training and validation cohorts, respectively.ConclusionOur RNA-seq and proteomic results provide valuable insight for understanding the immune cell composition of peripheral blood of patients with PDAC.

With the rapid development of the automobile industry and increasing demand for automobiles, consumers also have more and more car requirements. They do not only consider energy, safety and price but also expect a good indoor environment and improved thermal comfort. In order to construct and apply a thermal comfort evaluation method suitable for Chinese consumers, a relationship between the equivalent space temperature and the thermal sensation was obtained by collecting characteristic thermal sensation data of the human body in different environments. An evaluation method based on whole-body and local thermal comfort sensation characteristics for adult Chinese males and females was designed, At the same time, the study found that women's tolerance for thermal sensation is lower and the thermal comfort zone is relatively smaller. In order to explore the accuracy of the evaluation method and a typical non-uniform vehicular environment was selected to verify the findings. The local and whole-body equivalent space temperature of the co-driver seat in the vehicle was tested with a warm dummy, through which whole-body and local thermal sensation values were predicted. The local and whole-body thermal sensation was tested with subjects' subjective experience under the same conditions. The predicted thermal sensation obtained through the equivalent space temperature was compared to the subjective experience results, and the correlation reached up to 0.911, while significance (two-tailed) only reached 0.002, it was possible to predict the thermal sensation vote within an accuracy level of 0.3 point, further proving the accuracy of the whole-body and local thermal comfort evaluation method for adult Chinese male thermal sensation characteristics as proposed in this paper. The results obtained in this study can be used to predict environmental thermal comfort.

Multilevel ferroelectric field−effect transistors (FeFETs) integrated with HfO2−based ferroelectric thin films demonstrate tremendous potential in high−speed massive data storage and neuromorphic computing applications. However, few works have focused on the stability of the multiple memory states in the HfO2−based FeFETs. Here we firstly report the write/read disturb effects on the multiple memory states in the Hf0.5Zr0.5O2 (HZO)−based FeFETs. The multiple memory states in HZO−based FeFETs do not show obvious degradation with the write and read disturb cycles. Moreover, the retention characteristics of the intermediate memory states in HZO−based FeFETs with unsaturated ferroelectric polarizations are better than that of the memory state with saturated ferroelectric polarization. Through the deep analysis of the operation principle of in HZO−based FeFETs, we speculate that the better retention properties of intermediate memory states are determined by the less ferroelectric polarization degradation and the weaker ferroelectric polarization shielding. The experimental and theoretical evidences confirm that the long−term stability of the intermediate memory states in HZO−based FeFETs are as robust as that of the saturated memory state, laying a solid foundation for their practical applications.

Objective To establish a method for detecting human immunoglobulin Fc function based on surface plasmon resonance technology. Methods Based on the characteristic that FcγRI can be binded to the Fc segment of IgG, the affinity constant of the sample was detected by surface plasmon resonance, and its Fc function was the KD ratio of the sample to the standard. The method was validated for specificity/specificity, precision and robustness. The method and the pharmacopoeia method were used to detect the Fc function of 30 human immunoglobulins, and the correlation and consistency of the detection results were analyzed. Results The method validation results showed that this method has strong specificity/specificity (t values were 0.15, 0.22, both P>0.05), good precision (CV value 5.37%~10.69%) and good robustness (CV value 10.06%). The detection results of this method and the pharmacopoeia method have high correlation (r=0.96, P

Spatiotemporal fusion technology effectively improves the spatial and temporal resolution of remote sensing data by fusing data from different sources. Based on the strong time-series correlation of pixels at different scales (average Pearson correlation coefficients > 0.95), a new long time-series spatiotemporal fusion model (LOTSFM) is proposed for land surface temperature data. The model is distinguished by the following attributes: it employs an extended input framework to sidestep selection biases and enhance result stability while also integrating Julian Day for estimating sensor difference term variations at each pixel location. From 2013 to 2022, 79 pairs of Landsat8/9 and MODIS images were collected as extended inputs. Multiple rounds of cross-validation were conducted in Beijing, Shanghai, and Guangzhou with an all-round performance assessment (APA), and the average root-mean-square error (RMSE) was 1.60 °C, 2.16 °C and 1.71 °C, respectively, which proved the regional versatility of LOTSFM. The validity of the sensor difference estimation based on Julian days was verified, and the RMSE accuracy significantly improved (p < 0.05). The accuracy and time consumption of five different fusion models were compared, which proved that LOTSFM has stable accuracy performance and a fast fusion process. Therefore, LOTSFM can provide higher spatiotemporal resolution (30 m) land surface temperature research data for the evolution of urban thermal environments and has great application potential in monitoring anthropogenic heat pollution and extreme thermal phenomena.

A person spends about a third of their life sleeping, and high sleep quality is very important for health. Environmental factors are one of the most important factors affecting sleep quality, and indoor carbon dioxide (CO2) concentration while sleeping has a significant effect on sleep quality. In an indoor bedroom with no open windows and no fresh air system, different numbers of people sleeping will lead to changes in indoor CO2 concentration. In order to study the changes in sleep quality caused by differences in CO2 concentration, experimental research was performed. Objective sleep quality data are collected with polysomnography (PSG) and a subjective questionnaire. The sleep quality of the subjects is tested under three different CO2 concentration levels; the average carbon dioxide concentration of three conditions is 680, 920, and 1350 ppm, which simulate a room with 1, 2, and 3 people sleeping, respectively. Other environment parameters are controlled as follows: test environment temperature is 26 ± 0.5 °C, relative humidity is 50 ± 5%, there is no obvious heat source in the test room, and the radiation temperature and air temperature difference is less than 1 °C. A total of 30 subjective tests were carried out with 10 subjects; the test lasted more than one month. The data subsequently underwent statistical analysis to determine the influence of CO2 concentration on sleep quality. The results show that as the CO2 concentration level increased, the sleep quietness and satisfaction of the subjects gradually decreased, the sleep duration gradually decreased, and symptoms such as throat discomfort, dyspnea, dry and itchy skin, difficulty falling asleep, difficulty waking up, congested nose and bad air smell become more obvious. The PSG test results showed that CO2 concentration has a significant impact on the proportion of the N3 period. According to the group of CO2 concentration conditions, the mean of the N3 period proportion under the conditions of one person, two persons, and three persons is 20.4%, 17.3%, and 14.4%, respectively. Finally, there was also an increase in turning over or awakening during sleep, indicating that sleep quality was reduced under higher CO2 concentrations.

The rapid and effective fabrication of polyvinyl alcohol (PVA) hydrogels with good mechanical properties is of great significance yet remains a huge challenge. The preparation of PVA hydrogels via the conventional cyclic freeze–thaw method is intricate and time-intensive. In this study, a pioneering approach involving the utilization of low-temperature continuous freezing is introduced to produce a novel PVA-ethylene glycol (EG) gel. Fourier transform infrared (FTIR) spectroscopy, X-ray diffractometry (XRD) and scanning electron microscopy (SEM) confirm that with the assistance of EG, PVA molecular chains can self-assemble to generate an abundance of microcrystalline domains at low temperatures, thus improving the mechanical properties of PVA-EG gel. Remarkably, when the mass ratio of H2O/EG is 4:6, the gel’s maximum tensile strength can reach 2.5 MPa, which is much higher than that of PVA gels prepared via the freeze–thaw method. The preparation process of PVA-EG gel is simple, and its properties are excellent, which will promote the wide application of PVA tough gel in many fields.

HfO2-based ferroelectric films are attracting a great deal of attention. Here, the authors conclude that the performance degradation and the possible rejuvenation are ascribed to the reversible transition between polar and antipolar phases.

Decalcified bone matrix has great potential and application prospects in the repair of bone defects due to its good biocompatibility and osteogenic activity. In order to verify whether fish decalcified bone matrix (FDBM) has similar structure and efficacy, this study used the principle of HCl decalcification to prepare the FDBM by using fresh halibut bone as the raw material, and then degreasing, decalcifying, dehydrating and freeze-drying it. Its physicochemical properties were analyzed by scanning electron microscopy and other methods, and then its biocompatibility was tested by in vitro and in vivo experiments. At the same time, an animal model of femoral defect in rats was established, and commercially available bovine decalcified bone matrix (BDBM) was used as the control group, and the area of femoral defect in rats was filled with the two materials respectively. The changes in the implant material and the repair of the defect area were observed by various aspects such as imaging and histology, and its osteoinductive repair capacity and degradation properties were studied. The experiments showed that the FDBM is a form of biomaterial with high bone repair capacity and lower economic cost than other related materials such as bovine decalcified bone matrix. FDBM is simpler to extract and the raw materials are more abundant, which can greatly improve the utilization of marine resources. Our results show that FDBM not only has a good repair effect on bone defects, but also has good physicochemical properties, biosafety and cell adhesion, and is a promising medical biomaterial for the treatment of bone defects, which can basically meet the clinical requirements for bone tissue repair engineering materials.

Gate-all-around (GAA) structures are important for future logic devices and 3D-DRAM. Inner-spacer cavity etching and channel release both require selective etching of Si0.7Ge0.3. Increasing the number of channel-stacking layers is an effective way to improve device current-driving capability and storage density. Previous work investigated ICP selective etching of a three-cycle Si0.7Ge0.3/Si multilayer structure and the related etching effects. This study focuses on the dry etching of a 15-cycle Si0.7Ge0.3/Si multilayer structure and the associated etching effects, using simulation and experimentation. The simulation predicts the random effect of lateral etching depth and the asymmetric effect of silicon nanosheet damage on the edge, both of which are verified by experiments. Furthermore, the study experimentally investigates the influence and mechanism of pressure, power, and other parameters on the etching results. Research on these etching effects and mechanisms will provide important points of reference for the dry selective etching of Si0.7Ge0.3 in GAA structures.

This study investigated the impact of a multiple epoxy chain extender (ADR) on the rheological behavior, crystallization, and mechanical properties of polyglycolic acid (PGA). Tests of the torque and melt mass flow rate and dynamic rheological analysis were conducted to study the rheological behavior of PGA modified with ADR. The rheological results of the modified PGA showed a significantly increased viscosity and storage modulus with an increase in the ADR amount, which could be attributed to the chain extension/branching reactions between PGA and ADR. It was proved that ADR could be used as an efficient chain extender for tailoring the rheological performance of PGA. The Han plot of the modified PGA showed a transition of viscous behavior to elastic behavior, while the ADR content was increased from 0 to 0.9 phr. The formation of long-chain branches (LCBs) was confirmed via the Cole–Cole plot and weighted relaxation spectrum, wherein the LCBs substantially changed the rheological behavior of the modified PGA. The vGP plots predicted a star-type topological structure for the LCBs. The results of non-isothermal crystallization kinetics suggested that the crystallization of the modified PGA was predominantly homogeneous nucleation and three-dimensional growth. The crystallinity decreased slightly with the increase in the ADR amount. Compared to neat PGA, the modified PGA samples exhibited better tensile and flexural performances.

Background: Carotid artery stenosis has long been a critical cause of stroke and death, and it can seriously affect the life quality. Transcarotid artery revascularization (TCAR) and carotid endarterectomy (CEA) are both feasible therapies for this disease. This systematic review and meta-analysis aim to evaluate if the efficacy of the two approaches is comparable.Methods: Clinical studies up to March 2021 were searched through PubMed, Embase, and Scopus from a computer. The screening process was designed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) statement. Newcastle-Ottawa Scale (NOS) was used for methodological quality assessment of works of literature meeting the inclusion criteria, and Review Manager 5.4 was used for data synthesis. The I2 statistic was performed to measure the heterogeneity, and M-H/I-V fixed or random model was utilized depending on the I2 value. The evidence evaluation was accomplished based on grades of recommendation, assessment, development, and evaluation (GRADE) online tool.Results: A total of 14,200 subjects (six comparative studies) were finally included in this pooled study. There is no statistical discrepancy between the two treatments on reducing stroke/death/myocardial infarction (odds ratio [OR] 0.85, 95% CI 0.67–1.07), stroke (OR 1.03, 95% CI 0.77–1.37), or death (OR 1.14, 95% CI 0.67–1.94). Besides, TCAR is associated with a lower incidence of myocardial infarction (P = 0.004), cranial nerve injury (P < 0.00001), and shorter procedure time (P < 0.00001) than CEA among the overall cohort.Conclusions: TCAR is a rapidly developing treatment that reaches a comparable prognosis to CEA and significantly reduces the risk of myocardial infarction under the well-matched condition, which is a dependable choice for patients with carotid stenosis.

Distributed fiber optic sensing (DFS) systems are an effective method for long-distance pipeline safety inspections. Highly accurate vibration signal identification is crucial to DFS. In this paper, we propose an end-to-end high-accuracy fiber optic vibration signal detection and identification algorithm by extracting features from the time domain and frequency domain by a one-dimensional convolutional neural network and two-dimensional convolutional neural network, respectively, and introducing a self-attentive mechanism to fuse the features of multiple modes. First, the raw signal is segmented and normalized according to the statistical characteristics of the vibration signal combined with the distribution of noise. Then, the one-dimensional sequence of vibration signal and its two-dimensional image generated by short-time Fourier transform are input to the one-dimensional convolutional neural network and two-dimensional neural network, respectively, for automatic feature extraction, and the features are combined by long and short-time memory. Finally, the multimodal features generated from the time and frequency domains are fused by a multilayer TransformerEncoder structure with a multiheaded self-attentive mechanism and fed into a multilayer perceptron for classification. Experiments were conducted on an urban field database with complex noise and achieved 98.54% accuracy, which demonstrates the effectiveness of the proposed algorithm.

Mixed obstacles have a great influence on the deflagration process of liquefied petroleum gas (LPG)-air premixed combustible gas with concentration gradient. The arrangement of mixed obstacles may further stimulate overpressure and flame propagation. In this work, based on experimental and numerical simulations, this paper analyzes the flame and overpressure, and mainly studies the coupling relationship among the explosion overpressure characteristics, the structure of flame and the speed of flame propagation. The result shows that when the rectangular obstacle is 100 mm away from the ignition source, not only the speed of flame is the fastest, but also the time required to reach the maximum over-pressure is the shortest. In this configuration, an elongated flame is formed between a rectangular obstacle and a flat obstacle, and an obvious backflow structure appears. In addition, the average growth rate of overpressure has a minimum value, reaching at −35 MPa/s. The existence of rectangular obstacles further stimulates the overpressure. When the rectangular obstacle is 400 mm away from the ignition source, the maximum overpressure value is the highest among the four configurations. Besides, the time when the maximum area of flame appears in the simulation is almost the same as the time when the maximum overpressure is obtained. In addition, the average growth rate of overpressure increases significantly after touching the rectangular obstacle, which coincides with the mutation time of the front tip of the flame, overpressure and area of flame after the flame encounters the rectangular obstacle. This research has an important theoretical guiding significance for preventing LPG leakage and explosion accidents in a long and narrow space.

The arrangement of obstacles can significantly impact the deflagration behavior of combustible gases. In the actual pipeline accident site, liquefied petroleum gas (LPG) and other gases often show non-uniform distribution after leakage owing to diffusion and gravity, and the deflagration mechanism is also more complex. In this paper, based on the non-uniform distribution of combustible gases, the flame behavior and overpressure characteristics of LPG–air combustible gas deflagration are carried out by a combination of experiments and numerical simulations with obstacles arranged in increasing and decreasing blockage height. The results show that in the increasing blockage height arrangement, the flame forms a “straw hat” cavity, finally forming an elliptical region. In the decreasing blockage height arrangement, the flame appears as a “ribbon-shaped” narrow, blank area, which gradually becomes longer with time. By observing the overpressure and the structure of flame propagation in the coupled state, it is found that the explosion overpressure is maximum when the height of the obstacle is consistent, and the moment of the maximum area of flame appears slightly earlier than the appearance of the maximum overpressure peak. At the same time, without considering the change in height of the obstacle, the three arrangements all have an accelerating effect on the flame of deflagration. And the decreasing blockage height arrangement condition has the most obvious effect on the flame acceleration, which makes the peak of area of flame and the overpressure peak appear at first, and finally leads to the formation of a positive feedback mechanism among the speed of flame propagation, the area of flame and overpressure. In addition, in the case of the non-uniform distribution of combustible gases, the acceleration obtained by the flame at the initial stage is very important for the overall acceleration of the flame. The results of this paper can provide a reference for the placement of equipment and facilities in long and narrow spaces such as various pipe galleries, and to make predictions about the impact of the shape of some objects on the explosion and provide a theoretical basis for the prevention and management of gas explosions.

Urban environments have a strong influence on the land surface temperature (LST) in urban areas. Understanding the relationship between LST and urban environmental factors can help develop effective strategies to reduce high LSTs in urban areas, which is critical for mitigating the urban heat island effect. Previous studies have focused on the correlation between LST and the environmental factors that drive its formation, without considering the influences of the neighboring environment and the vertical expansion of highly urbanized areas. Notably, the correlation between LST and its neighboring environment in different seasons remains unclear. In this study, we selected central Beijing in China as our study area and employed the moving window method to characterize the environmental factors of the neighboring environment of the central LST cell. We explored eight environmental factors from three layers: normalized difference vegetation index (NDVI), normalized difference built-up index (NDBI), modified normalized difference water index (MNDWI), building density (BD), building height (BH), building volume (BV), sky view factor (SVF), and road density (RD). The Pearson correlation and extreme gradient boosting (XGB) regression methods were applied to measure the correlation between LST and the different factors in moving windows of different sizes. The results indicated that the correlation between NDVI, MNDWI, and LST was considerably different in the winter and other seasons. However, NDBI was positively correlated with LST in all seasons, although the correlation was strongest/weakest in summer/winter. Among building-related factors, BD and BH were more strongly correlated with LST, and the positive/negative correlation between BD/BH and LST was stronger in summer/winter. The correlation between LST and its neighboring environment varied with increasing window size, and this variation differs significantly between winter and other seasons. In spring, summer, and autumn, the strength of the correlation between LST and its neighboring environment showed an “inverted V” pattern with increasing window size. The optimal spatial scales to explore the influence of neighboring environments on the LST of 30-m cells were 210 m and 270 m. This study revealed the seasonal correlation between LST and its neighboring environment while explaining the variation at a spatial scale. Notably, this study can provide a new perspective for understanding the driving mechanism of the urban thermal environment, while contributing to its scientific optimization and management.

Population migration, accompanied by urbanization, has led to an increase in the urban-settled population. However, quantitative studies on the distribution of urban-settled population, especially at fine scale, are limited. This study explored the relationship between characteristics of human perceived environment and the distribution of settled population, and proposed a quantitative method to predict the distribution trend of settled population. Through the semantic segmentation of street view images and accessibility calculation based on traffic isochrone and points-of-interest, we determined human perception factors. The influence of human perception factors was quantified using the geographic detector method, and the settlement intention index (SII) was constructed combining the analytic hierarchy process to predict the distribution trend of settled population. The results indicated the following. (1) Human perception was one of the important factors influencing the distribution of urban-settled population, and the cycling accessibility to traffic facilities was closely related to the distribution of settled population. (2) The accessibility and visibility of green space with low independent influence portrayed a strong enhancement on the interactive effect of other perception factors. (3) The SII mapping of Beijing showed that the SII was reliable. This study analyzes the role of human perception in shaping the environment, and provides reference for population-related urban planning problems.

Chlorella has become an important raw material for biodiesel production in recent years, and Chlorella sp. TLD6B, a species with high lipid concentrations and high salt and drought tolerance, has been cultivated on a large scale. To explore the lipid accumulation of Chlorella sp. TLD6B and its relationship to external NaCl concentrations, we performed physiological measurements and genome-wide gene expression profiling under different levels of salt stress. Chlorella sp. TLD6B was able to tolerate high levels of salt stress (0.8 M NaCl addition). Lipid concentrations initially increased and then decreased as salt stress increased and were highest under the addition of 0.2 M NaCl. Comparative transcriptomic analysis revealed that salt stress enhanced the expression of genes related to sugar metabolism and fatty acid biosynthesis (the ACCases BC and BCCP, KAS II, and GPDHs involved in TAG synthesis), thereby promoting lipid accumulation under the addition of 0.2 M NaCl. However, high salinity inhibited cell growth. Expression of three SADs, whose encoded products function in unsaturated fatty acid biosynthesis, was up-regulated under high salinity (0.8 M NaCl addition). This research clarifies the relationship between salt tolerance and lipid accumulation and promotes the utilization of Chlorella sp. TLD6B.

China has the largest W reserves in the world, which are mainly concentrated in south China. Although previous studies have been carried out on whether mantle material is incorporated in granites associated with W deposits, the conclusions have been inconsistent. However, rare gas isotopes can be used to study the contribution of mantle-to-W mineralization. In this paper, we investigated the He and Ar isotope compositions of fluid inclusions in pyrite and wolframite from the Xingluokeng ultra-large W-Mo deposit to evaluate the origin of ore-forming fluids and discuss the contribution of the mantle-to-tungsten mineralization. The He-Ar isotopic compositions showed that the 3He/4He ratios of the ore-forming fluid of the Xingluokeng deposit ranged from 0.14 to 1.01 Ra (Ra is the 3He/4He ratio of air, 1 Ra = 1.39 × 10−6), with an average of 0.58 Ra, which is between the 3He/4He ratios of mantle fluids and crustal fluids, suggesting that the mantle-derived He was added to the mineralizing fluid, with a mean of 8.7%. The 40Ar/36Ar ratios of these samples ranged from 361 to 817, with an average of 578, between the atmospheric 40Ar/36Ar and the crustal and/or mantle 40Ar/36Ar. The results of the He-Ar isotopes from Xingluokeng W-Mo deposit showed that the ore-forming fluid of the deposit was not the product of the evolution of pure crustal melt. The upwelling mantle plays an important role in the formation of tungsten deposits.

In this work, low-temperature Schottky source/drain (S/D) MOSFETs are investigated as the top-tier devices for 3D sequential integration. Complementary Schottky S/D FinFETs are successfully fabricated with a maximum processing temperature of 500 °C. Through source/drain extension (SDE) engineering, competitive driving capability and switching properties are achieved in comparison to the conventional devices fabricated with a standard high-temperature (≥1000 °C) process flow. Schottky S/D PMOS exhibits an ON-state current (ION) of 76.07 μA/μm and ON-state to OFF-state current ratio (ION/IOFF) of 7 × 105, and those for NMOS are 48.57 μA/μm and 1 × 106. The CMOS inverter shows a voltage gain of 18V/V, a noise margin for high (NMH) of 0.17 V and for low (NML) of 0.43 V, with power consumption less than 0.9 μW at VDD of 0.8 V. Full functionality of CMOS ring oscillators (RO) are further demonstrated.

We analyze theoretically biaxially tensile strained Ge/Si0.18Ge0.82 multiple quantum wells (MQWs) for electro-absorption modulators with low polarization sensitivities. The difference between TE and TM polarized momentum matrix elements and absorption spectra are discussed. Our calculation indicates that polarization independent electroabsorption can be achieved by introducing 0.78% biaxial tensile strain in the buffer layer. The simulation results show that the maximum polarization independent absorption contrast ratio is 7.6 dB under 0 V/2 V operation at 1485 nm. The absorption contrast ratio is over 6 dB for both TE and TM polarizations with the wavelength ranging from 1467 to 1489 nm. The proposed scheme provides a promising approach to design highly efficient polarization independent Ge/SiGe MQWs electroabsorption modulators for on-chip optical transmission applications.

Guinea pig represents a highly suitable animal model for study of Mycobacterium tuberculosis(Mtb)infection, as it demonstrates similarities to Mtb pulmonary infection in humans. It is known that guinea pigs can be efficiently infected by the respiratory or subcutaneous exposure to Mtb. However, information on Mtb infection through oral route is almost absent in the literature. Here, we examined whether guinea pigs can be infected by drinking Mtb containing water. Our findings confirmed that the guinea pigs could be infected with Mtb via drinking virulent water. The infected guinea pigs developed uniform oval-craterform ulcers at the 30th day after infection. Bacterial cultures showed Mtb growth in the lungs and spleens from the guinea pigs infected with high dose of Mtb. In addition, the infected animals had histopathological granulomatous lesions in lungs, spleens and mesenteric lymph nodes. We provide the compelling evidence that the guinea pigs could be infected by drinking Mtb containing water. The clinical and pathological observations in the infected animals were similar to those found in guinea pigs infected via the respiratory or subcutaneous routes.

Clinical mastitis in sheep has gravely restrained production performance for a long time. Knowledge of mechanisms of its pathogenesis and resistance in meat sheep mammary gland with clinical mastitis are not yet understood, especially for clinical mastitis caused by natural infection. In this work, RNA-sequencing was firstly used to screen the differentially expressed genes (DEGs) in clinical mastitic mammary tissues (CMMTs) when compared with healthy mammary tissues (HMTs) from meat sheep flocks. We identified 420 DEGs including 316 upregulated and 104 downregulated genes in CMMTs. Gene ontology annotation revealed these DEGs were mainly engaged in immune response and inflammation response. Pathway enrichment showed they were primarily enriched in pathways relevant to inflammation, immune response and metabolism. Alternative splicing analysis showed most common differential splicing genes in CMMTs and HMTs were implicated in immune response. Immunostaining for three immune response-related proteins encoded by DEGs were mainly observed in mammary epithelium from both CMMTs and HMTs, and their positive signals were more intensive in CMMTs than those in HMTs. These findings provide experimental basis and reference for further researching the molecular genetic mechanisms, particularly immune defence mechanisms, of sheep mammary gland during clinical mastitis.

Panels using solar power require high reliability, and the residual stress in the solar panel has an important effect on its reliability and lifetime. The finite element method was adopted to simulate the impacts of the rectangular solar panel encapsulation process parameters, such as the elastic modulus, the thickness of adhesive, and the curing temperature on the residual stress in the solar cell module. The results show that the residual stress in the solar cell module increases linearly with the increase in these three factors. The residual strain is consistent with that of the stress. The generation mechanism and distribution evolution of stress are discussed in detail. Both the thickness and the elastic modulus of the silicone rubber have significant impact on the residual stress. However, the influence of the curing temperature is less observable.

The Chuankou tungsten ore field is situated in the central area of the Xuefeng Uplift Belt in South China. The deposit is characterized by two types of tungsten mineralization: quartz-scheelite veins in both the Neoproterozoic Banxi Group and Devonian Yanglin’ao Formation and quartz-wolframite (scheelite) veins in the Chuankou granite. The host rocks of the Chuankou tungsten Deposit of South China are similar to the stratigraphic sequence of Au-Sb-W deposits in the Xuefeng Uplift Belt. It is thus an appropriate location for the study of scheelite mineralization in the belt, especially the relative contributions of surrounding rocks, magma and hydrothermal fluids. Optical Microscope-Cathodoluminescene (OM-CL) and Laser Ablation Inductively Coupled Mass Spectrometers (LA ICPMS) were used to examine scheelite textures and trace element concentrations in the Chuankou deposits. Scheelite in quartz-scheelite veins was formed over three generations. In situ LA-ICPMS trace elemental analyses of scheelite I show light rare earth element (LREE)-rich REE patterns and negative Eu anomalies, suggesting a relatively close fluid system. Significantly positive Eu anomalies of scheelite II and III indicate variable degrees of addition of meteoric water during scheelite precipitation. Therefore, ore-forming fluids of the Chuankou deposit were dominantly magma-derived, with different contributions of recycled meteoric water in the surrounding strata.

Heavily doped polysilicon layers have been widely used in the fabrication of microelectromechanical systems (MEMS). However, the investigation of high selectivity, anisotropy, and excellent uniformity of heavily doped polysilicon etching is limited. In this work, reactive ion etching of undoped and heavily doped polysilicon-based hydrogen bromide (HBr) plasmas have been compared. The mechanism of etching of heavily doped polysilicon is studied in detail. The final results demonstrate that the anisotropy profile of heavily doped polysilicon can be obtained based on a HBr plasma process. An excellent uniformity of resistance of the thermocouples reached ± 2.11%. This technology provides an effective way for thermopile and other MEMS devices fabrication.

The international technology roadmap of semiconductors (ITRS) is approaching the historical end point and we observe that the semiconductor industry is driving complementary metal oxide semiconductor (CMOS) further towards unknown zones. Today’s transistors with 3D structure and integrated advanced strain engineering differ radically from the original planar 2D ones due to the scaling down of the gate and source/drain regions according to Moore’s law. This article presents a review of new architectures, simulation methods, and process technology for nano-scale transistors on the approach to the end of ITRS technology. The discussions cover innovative methods, challenges and difficulties in device processing, as well as new metrology techniques that may appear in the near future.

Stacked SiGe/Si structures are widely used as the units for gate-all-around nanowire transistors (GAA NWTs) which are a promising candidate beyond fin field effective transistors (FinFETs) technologies in near future. These structures deal with a several challenges brought by the shrinking of device dimensions. The preparation of inner spacers is one of the most critical processes for GAA nano-scale transistors. This study focuses on two key processes: inner spacer film conformal deposition and accurate etching. The results show that low pressure chemical vapor deposition (LPCVD) silicon nitride has a good film filling effect; a precise and controllable silicon nitride inner spacer structure is prepared by using an inductively coupled plasma (ICP) tool and a new gas mixtures of CH2F2/CH4/O2/Ar. Silicon nitride inner spacer etch has a high etch selectivity ratio, exceeding 100:1 to Si and more than 30:1 to SiO2. High anisotropy with an excellent vertical/lateral etch ratio exceeding 80:1 is successfully demonstrated. It also provides a solution to the key process challenges of nano-transistors beyond 5 nm node.

Semiconductor nanowires have great application prospects in field effect transistors and sensors. In this study, the process and challenges of manufacturing vertical SiGe/Si nanowire array by using the conventional lithography and novel dry atomic layer etching technology. The final results demonstrate that vertical nanowires with a diameter less than 20 nm can be obtained. The diameter of nanowires is adjustable with an accuracy error less than 0.3 nm. This technology provides a new way for advanced 3D transistors and sensors.