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Blood oxygen saturation meter is a tool used to monitor the state of oxygen saturation in the blood and also the patient's heart rate (BPM) and to assist in the physical assessment of the patient without going through blood gas analysis. Oxygen saturation measuring devices usually use the difference in the wavelengths of red and infrared led light that will be captured by the photodiode. The purpose of this research is to make a pulse oximeter equipped with a display of SPO2, BPM values ​​and an additional SP02 signal. The design of this measuring instrument uses the MAX30100 sensor, the minimum system circuit of Arduino ATmega328p and OLED (Organic Light-Emitting Diode). Data from the MAX30100 sensor enters the I2C pin on the minimum Arduino system, then the microcontroller is processed to produce the percentage of SPO2 value, BPM value, and SPO2 signal which is then displayed on the OLED. The test is done by comparing the module with standard measuring instruments which produces the largest % error of 0.81% for Spo2 and 0.87% for BPM. The error presentation is obtained from factor measurements, if there is finger movement it will cause a large error. From the results obtained, the tool is still feasible to use because in the "Guidelines for Testing and Calibrating Medical Devices" Ministry of Health RI 2001, the maximum limit in the pulse oximeter error tolerance is for Spo2 1% and BPM 5%.

Aims Our previous study demonstrated that Ca2+ influx through the Orai1 store-operated Ca2+ channel in macrophages contributes to foam cell formation and atherosclerosis via the calcineurin–ASK1 pathway, not the classical calcineurin–nuclear factor of activated T-cell (NFAT) pathway. Moreover, up-regulation of NFATc3 in macrophages inhibits foam cell formation, suggesting that macrophage NFATc3 is a negative regulator of atherogenesis. Hence, this study investigated the precise role of macrophage NFATc3 in atherogenesis. Methods and results Macrophage-specific NFATc3 knockout mice were generated to determine the effect of NFATc3 on atherosclerosis in a mouse model of adeno-associated virus-mutant PCSK9-induced atherosclerosis. NFATc3 expression was decreased in macrophages within human and mouse atherosclerotic lesions. Moreover, NFATc3 levels in peripheral blood mononuclear cells from atherosclerotic patients were negatively associated with plaque instability. Furthermore, macrophage-specific ablation of NFATc3 in mice led to the atherosclerotic plaque formation, whereas macrophage-specific NFATc3 transgenic mice exhibited the opposite phenotype. NFATc3 deficiency in macrophages promoted foam cell formation by potentiating SR-A- and CD36-meditated lipid uptake. NFATc3 directly targeted and transcriptionally up-regulated miR-204 levels. Mature miR-204-5p suppressed SR-A expression via canonical regulation. Unexpectedly, miR-204-3p localized in the nucleus and inhibited CD36 transcription. Restoration of miR-204 abolished the proatherogenic phenotype observed in the macrophage-specific NFATc3 knockout mice, and blockade of miR-204 function reversed the beneficial effects of NFATc3 in macrophages. Conclusion Macrophage NFATc3 up-regulates miR-204 to reduce SR-A and CD36 levels, thereby preventing foam cell formation and atherosclerosis, indicating that the NFATc3/miR-204 axis may be a potential therapeutic target against atherosclerosis.

The aim of this paper is to study class number relations over function fields and the intersections of Hirzebruch-Zagier type divisors on the Drinfeld-Stuhler modular surfaces. The main bridge is a particular "harmonic" theta series with nebentypus. Using the strong approximation theorem, the Fourier coefficients of this series are expressed in two ways; one comes from modified Hurwitz class numbers and another gives the intersection numbers in question., DOCUMENTA MATHEMATICA, Vol 27 (2022), p. 1321-1368

A superconducting nanowire single-photon imager (SNSPI) uses a time-multiplexing method to reduce the readout complexity. However, due to the serial connection, the nanowire should be uniform so that a common bias can set all segments of the nanowire to their maximum detection efficiency, which becomes more challenging as the scalability (i.e., the length of the nanowire) increases. Here, we have developed a 64-pixel SNSPI based on amorphous Mo

Abnormally increased reactive oxygen species (ROS) are intimately related to the development and metastasis of cancer. Since hydrogen peroxide (H2O2) is a major component of ROS, molecular imaging and selective treatment in response to high H2O2 are intriguing for the management of cancers. Herein, we report novel self-assembly luminescent nanoparticles, which can be activated by H2O2, thereby serving as an effective nanotheranostics for luminescence imaging and in situ photodynamic therapy (PDT) of tumors with high H2O2. This functional nanomedicine was assembled from an amphiphilic conjugate (defined as CLP) based on chlorin e6 (Ce6) simultaneously conjugated with luminol and poly(ethylene glycol), exhibiting a well-defined core-shell nanostructure. Upon triggering by pathologically relevant levels of H2O2, CLP nanoparticles produced luminescence due to the luminol unit and simultaneous excitation of Ce6 by chemiluminescence resonance energy transfer, enabling in vitro and in vivo imaging of tumors with highly expressed H2O2. In addition, excited Ce6 can produce singlet oxygen (1O2) for in situ PDT of H2O2-high tumors and inhibiting lung metastasis, which was demonstrated by in vitro and in vivo experiments. Furthermore, preliminary studies revealed the biosafety of CLP nanoparticles. Consequently, the self-illuminating nanoparticles are promising for noninvasive imaging and therapy of tumors with high expression of H2O2.

A fan-out circuit is a basic block for scaling up digital circuits for overcoming the limited driving capability of a single logic gate. It is particularly important for superconducting digital circuits as the driving power is typically weak for having high energy efficiency. Here, we design and fabricate a fan-out circuit for a superconducting nanowire cryotron (nTron) digital circuit. A classic splitter tree architecture is adopted. To transmit switching signal and avoid crosstalk among nTrons, we introduced an “R–L–R” interface circuit. Experimentally, a two-stage splitter tree of a fan-out number of four was demonstrated. Correct operation was observed with a minimum bit error rate (BER) of 10−6. The bias margin was 10% at BER of 10−4. The average time jitter was 82 ps. Moreover, crosstalk was not observed. Based on these results, we envision that the fan-out circuit can be used in future development of superconducting-nanowire-based circuits.

Designing a spectrometer without the need for wavelength multiplexing optics can effectively reduce the complexity and physical footprint. On the basis of the computational spectroscopic strategy and combining a broadband-responsive dynamic detector, we successfully demonstrate an optics-free single-detector spectrometer that maps the tunable quantum efficiency of a superconducting nanowire into a matrix to build a solvable mathematical equation. Such a spectrometer can realize a broadband spectral responsivity ranging from 660 to 1900 nm. The spectral resolution at the telecom is sub-10 nm, exceeding the energy resolving capacity of existing infrared single-photon detectors. Meanwhile, benefiting from the optics-free setup, precise time-of-flight measurements can be simultaneously achieved. We have demonstrated a spectral LiDAR with eight spectral channels. This spectrometer scheme paves the way for applying superconducting nanowire detectors in multifunctional spectroscopy and represents a conceptual advancement for on-chip spectroscopy and spectral imaging.

As an important ecological barrier for the North China Plain, research on the spatial variations of soil nutrients in the southern foot of the Taihang Mountains is of great significance for the forestry ecological construction in this rocky mountainous area. With the typical slopes (the artificial forestland and the natural wild slope) of the southern foot of the Taihang Mountains as the research objects, we used the grid method to arrange sampling points, and combined classical statistics, geostatistics and constrained sorting methods to analyze the spatial variations of soil nutrients. The results showed that: 1) soil total carbon (TC) contents were 6.80-57.05 g·kg太行山南麓是我国华北平原的重要生态屏障,研究该区域土壤养分的空间变异性对土石山区林业生态建设具有重要意义。本研究以太行山南麓典型坡面(人工林坡地和自然荒坡地)为对象,采用网格法布设采样点,运用经典统计学、地统计学和约束性排序相结合的方法对土壤养分的空间变异性进行分析。结果表明: 1)太行山南麓的土壤全碳(TC)含量为6.80～57.05 g·kg

The effects of climate warming on boreal wetland plant structure and carbon (C) sequestration on local scale may be overestimated. An in situ passive warming experiment manipulated by open-top chambers and artificial nitrogen (N) addition was deployed in a lacustrine wetland of Xingkai Lake for 3 consecutive years. The annual changes and allocations of the aboveground biomass of Glyceria spiculosa, and decomposition dynamics of the total litter and cotton strips as standard references were observed. Results showed that the aboveground biomass was significantly affected by warming and increased from 99.43 ± 10.59 g m−2 (ambient) to 112.02 ± 8.08 g m−2 (ca. +1 °C) and 117.21 ± 9.92 g m−2 (ca. +2 °C). N addition had a more positive effect on the annual aboveground biomass accumulations than warming, for the relative importance weights of N addition were 2.60 and 1.49 times greater than warming in 2011 and 2013 respectively. Their main effects on the allocations had significant interannual variations, and their interaction effects were dependent on organ and year. The decomposition constant (k-value) of the litter and cotton strips were 0.747 yr−1 and 2.057 yr−1, respectively. Compared to warming and N addition, the internal quality characterized by Lignocellulose index and soil organisms reflected by litterbag size played overwhelming role in decomposition dynamics, with 2 orders greater of relative importance weights in 2011 and 2012. Our results highlight the importance of interannual variation for differentiating the contributions of external and internal factors to boreal wetland plant biomass accumulation and decomposition. Given the asymmetric responses of accumulation and decomposition, the C storage in the litter would increase in long term.

Multilayer superconducting nanowire single-photon detectors (SNSPDs) have exhibited several advantages, such as increased detection efficiency, reduced polarization sensitivity, and scaling up to large arrays. However, monolithic fabrication of such multilayer devices is challenging. In this work, a hybrid integration method has been introduced by etching SNSPDs into the membrane microchips, followed by the pick and place transferring process. This method has been verified by stacking two SNSPDs orthogonally. Both detectors show near saturated detection efficiencies and low timing jitters. Furthermore, thermal coupling effects have been observed between the two SNSPDs. The photon detection pulses from either detector can trigger the other one almost deterministically with a latency of several nanoseconds. This method offers a flexible way to fabricate multilayer SNSPDs or integrate them with other heterogeneous devices.

A superconducting nanowire single-photon imager (SNSPI) uses a time-multiplexing method to reduce the readout complexity. However, due to the serial connection, the nanowire should be uniform so that a common bias can set all segments of the nanowire to their maximum detection efficiency, which becomes more challenging as the scalability (i.e., the length of the nanowire) increases. Here, we have developed a 64-pixel SNSPI based on amorphous Mo80Si20 film, which yielded a uniform nanowire and slow transmission line. Adjacent detectors were separated by delay lines, giving an imaging field of 270 µm × 240 µm. Benefiting from the high kinetic inductance of Mo80Si20 films, the delay line gave a phase velocity as low as 4.6 µm/ps. The positions of all pixels can be read out with a negligible electrical cross talk of 0.02% by using cryogenic amplifiers. The timing jitter was 100.8 ps. Saturated internal quantum efficiency was observed at a wavelength of 1550 nm. These results demonstrate that amorphous film is a promising material for achieving SNSPIs with large scalability and high efficiency.

Background: Feature extraction from photoplethysmography (PPG) signals is an essential step to analyze vascular and hemodynamic information. Different morphologies of PPG waveforms from different measurement sites appear. Various phenomena of missing or ambiguous features exist, which limit subsequent signal processing. Methods: The reasons that cause missing or ambiguous features of finger and wrist PPG pulses are analyzed based on the concept of component waves from pulse decomposition. Then, a systematic approach for missing-feature imputation and ambiguous-feature resolution is proposed. Results: From the experimental results, with the imputation and ambiguity resolution technique, features from 35,036 (98.7%) of 35,502 finger PPG cycles and 36307 (99.1%) of 36,652 wrist PPG cycles can be successfully identified. The extracted features became more stable and the standard deviations of their distributions were reduced. Furthermore, significant correlations up to 0.92 were shown between the finger and wrist PPG waveforms regarding the positions and widths of the third to fifth component waves. Conclusion: The proposed missing-feature imputation and ambiguous-feature resolution solve the problems encountered during PPG feature extraction and expand the feature availability for further processing. More intrinsic properties of finger and wrist PPG are revealed. The coherence between the finger and wrist PPG waveforms enhances the applicability of the wrist PPG.

Combining organic fertilizer with chemical fertilizer may affect the microbial processes related to nitrous oxide (N

A weighted pulse decomposition analysis is proposed for cuffless blood pressure assessment. Five Gaussian waves are used for decomposition. The weighted least squares criterion is adopted for optimization. Instead of applying weights to certain crest and trough points of the pulse signal from fingertip photoplethysmogram, we apply the weights to the pulse segment that is recognized informative for vascular age, vessel stiffness, and blood pressure. In addition, the boundary constraints of the Gaussian parameters are carefully set so that the normalized root mean square error (NRMSE) between the original PPG and synthesized PPG can be kept acceptable. From the results, we can see that weighting makes the decomposition stable and the variances of Gaussian parameters reduced. Besides, the modified boundary constraints improve the NRMSE. Furthermore, the correlation between the blood pressures and the features from weighted pulse decomposition analysis is enhanced and thus the proposed approach is helpful to blood pressure assessment.

Liver injury can result in different hepatic diseases such as fatty liver, liver fibrosis, hepatitis, and liver failure, which are mainly responsible for global mortality and morbidity. Early diagnosis is critical for the treatment of liver diseases. Herein we report luminescence imaging of neutrophil-mediated acute liver injury, including alcoholic liver injury (ALI) and acute liver failure (ALF). To this purpose, a biodegradable luminescent material was developed by chemical functionalization of a cyclic oligosaccharide, which can be produced into nanoprobes (defined as LaCD NPs). Luminescence of LaCD NPs was dependent on the level of reactive oxygen species and myeloperoxidase (MPO). Correspondingly, activated neutrophils could be specifically imaged by LaCD NPs, and the luminescent signal was positively associated with the neutrophil count. In mouse models of ALI and ALF, LaCD NPs enabled precise quantification and tracking of neutrophils in livers. In both cases, changes in the luminescence intensity are consistent with time-dependent profiles of neutrophils, MPO, and other parameters relevant to the pathogenesis of liver injury. Moreover, the luminescence imaging capacity of LaCD NPs can be additionally improved by surface functionalization with a neutrophil-targeting peptide. In addition, preliminary in vitro and in vivo studies demonstrated good safety of LaCD NPs. Consequently, LaCD NPs can be further developed as an effective and biocompatible luminescent nanoprobe for in vivo dynamic detection of the development of neutrophil-mediated acute liver injury. It is also promising for diagnosis of other neutrophil-associated liver diseases.

Nonalcoholic fatty liver disease (NAFLD) is the most prevalent form of chronic liver disease, and the mechanisms underpinning its pathogenesis have not been completely established. Transmembrane member 16A (TMEM16A), a component of the Ca2+‐activated chloride channel (CaCC), has recently been implicated in metabolic events. Herein, TMEM16A is shown to be responsible for CaCC activation in hepatocytes and is increased in liver tissues of mice and patients with NAFLD. Hepatocyte‐specific ablation of TMEM16A in mice ameliorates high‐fat diet‐induced obesity, hepatic glucose metabolic disorder, steatosis, insulin resistance, and inflammation. In contrast, hepatocyte‐specific TMEM16A transgenic mice exhibit the opposite phenotype. Mechanistically, hepatocyte TMEM16A interacts with vesicle‐associated membrane protein 3 (VAMP3) to induce its degradation, suppressing the formation of the VAMP3/syntaxin 4 and VAMP3/synaptosome‐associated protein 23 complexes. This leads to the impairment of hepatic glucose transporter 2 (GLUT2) translocation and glucose uptake. Notably, VAMP3 overexpression restrains the functions of hepatocyte TMEM16A in blocking GLUT2 translocation and promoting lipid deposition, insulin resistance, and inflammation. In contrast, VAMP3 knockdown reverses the beneficial effects of TMEM16A downregulation. This study demonstrates a role for TMEM16A in NAFLD and suggests that inhibition of hepatic TMEM16A or disruption of TMEM16A/VAMP3 interaction may provide a new potential therapeutic strategy for NAFLD., Transmembrane member 16A (TMEM16A) is an essential component of the hepatocyte calcium‐activated chloride channel and is increased in livers with hepatic steatosis. It interacts with vesicle‐associated membrane protein 3 to disrupt soluble Nethylmaleimide‐sensitive factor attachment protein receptors formation, thereby limiting glucose transporter 2 translocation and hepatic glucose uptake. Liver‐specific deficiency of TMEM16A ameliorates glucose metabolic disorder and nonalcoholic fatty liver disease.

Photon number resolving (PNR) is an important capacity for detectors working in quantum and classical applications. Although a conventional superconducting nanowire single-photon detector (SNSPD) is not a PNR detector, by arranging nanowires in a series array and multiplexing photons over space, such series PNR-SNSPD can gain quasi-PNR capacity. However, the accuracy and maximum resolved photon number are both limited by the signal-to-noise (SNR) ratio of the output pulses. Here, we introduce a matched filter, which is an optimal filter in terms of SNR for SNSPD pulses. Experimentally, compared to conventional readout using a room-temperature amplifier, the normalized spacing between pulse amplitudes from adjacent photon number detections increased by a maximum factor of 2.1 after the matched filter. Combining with a cryogenic amplifier to increase SNR further, such spacing increased by a maximum factor of 5.3. In contrast to a low pass filter, the matched filter gave better SNRs while maintaining good timing jitters. Minimum timing jitter of 55 ps was obtained experimentally. Our results suggest that the matched filter is a useful tool for improving the performance of the series PNR-SNSPD and the maximum resolved photon number can be expected to reach 65 or even large.

The late Paleozoic Tuwu porphyry Cu deposit is located in the Dananhu–Tousuquan arc in Eastern Tianshan, northwest China. In this study, we present new apatite fission track data of the intermediate-felsic intrusions (i.e., diorite porphyry, tonalite porphyry, quartz porphyry, and quartz albite porphyry) from Tuwu, together with inverse thermal modeling and previous study, to reveal exhumation and cooling history. Apatite fission track length analyses indicate that the track annealing type belongs to a transition from the undisturbed basement distribution to the mixed distribution. As a whole, two phases of cooling were recognized from inverse thermal modeling including a protracted slow cooling period with an average cooling rate of 0.32 ℃/Ma during late Cretaceous to Miocene and moderate slow cooling with a cooling rate of 1.89 ℃/Ma from Miocene to the present day. The first phase of cooling can be mainly attributed to a tectonically quiet and arid to semiarid climate whereas the later phases of cooling related to structural activation occurred at ~25–15 Ma in response to a distant effect of the India-Eurasia convergence. In addition, the exhumation rates of Tuwu intrusions from the two stages are 10.7 m/Ma and 63 m/Ma, respectively. The calculated total exhumation thickness at Tuwu is 1.2–2.3 km since late Cretaceous. Based on the integration of stratigraphic sequence, tectonic setting, paleogeographic, geochronology, and low temperature thermochronology study, we suggest that the thick deposition of coal-bearing stratum during the early and middle Jurassic, the extended duration slow cooling and exhumation controlled by arid to semiarid climate, and the differential lifting movement of fault blocks are key factors for preservation of the early Carboniferous Tuwu Cu deposit.