39 results on '"Jangpatarapongsa K"'
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2. Immune response to Plasmodium vivax has a potential to reduce malaria severity
- Author
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Chuangchaiya, S, Jangpatarapongsa, K, Chootong, P, Sirichaisinthop, J, Sattabongkot, J, Pattanapanyasat, K, Chotivanich, K, Troye-Blomberg, Marita, Cui, L, Udomsangpetch, R, Chuangchaiya, S, Jangpatarapongsa, K, Chootong, P, Sirichaisinthop, J, Sattabongkot, J, Pattanapanyasat, K, Chotivanich, K, Troye-Blomberg, Marita, Cui, L, and Udomsangpetch, R
- Abstract
Summary Plasmodium falciparum infection causes transient immunosuppression during the parasitaemic stage. However, the immune response during simultaneous infections with both P. vivax and P. falciparum has been investigated rarely. In particular, it is not clear whether the host's immune response to malaria will be different when infected with a single or mixed malaria species. Phenotypes of T cells from mixed P. vivax-P. falciparum (PV-PF) infection were characterized by flow cytometry, and anti-malarial antibodies in the plasma were determined by an enzyme-linked immunosorbent assay. We found the percentage of CD3(+)delta2(+)-T cell receptor (TCR) T cells in the acute-mixed PV-PF infection and single P. vivax infection three times higher than in the single P. falciparum infection. This implied that P. vivax might lead to the host immune response to the production of effector T killer cells. During the parasitaemic stage, the mixed PV-PF infection had the highest number of plasma antibodies against both P. vivax and P. falciparum. Interestingly, plasma from the group of single P. vivax or P. falciparum malaria infections had both anti-P. vivax and anti-P. falciparum antibodies. In addition, antigenic cross-reactivity of P. vivax or P. falciparum resulting in antibodies against both malaria species was shown in the supernatant of lymphocyte cultures cross-stimulated with either antigen of P. vivax or P. falciparum. The role of delta2 +/- TCR T cells and the antibodies against both species during acute mixed malaria infection could have an impact on the immunity to malaria infection., authorCount :10
- Published
- 2010
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3. Composite Nanoparticles on the Natural Rubber Latex Glove for Reduction of Surface Friction and Cytotoxicity
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Kanjanathaworn, N., primary, Kaewsaneha, C., additional, Polpanich, D., additional, Jangpatarapongsa, K., additional, and Tangboriboonrat, P., additional
- Published
- 2012
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4. Immune response to Plasmodium vivax has a potential to reduce malaria severity
- Author
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Chuangchaiya, S., primary, Jangpatarapongsa, K., additional, Chootong, P., additional, Sirichaisinthop, J., additional, Sattabongkot, J., additional, Pattanapanyasat, K., additional, Chotivanich, K., additional, Troye-Blomberg, M., additional, Cui, L., additional, and Udomsangpetch, R., additional
- Published
- 2009
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5. Quantum dots as a fluorescent labeling tool for live-cell imaging of Leptospira .
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Tantiapibalkun Y, Nuchpun S, Mekseriwattana W, Limsampan S, Doungchawee G, Jangpatarapongsa K, Srikhirin T, and Katewongsa KP
- Subjects
- Fluorescent Dyes chemistry, Cadmium Compounds chemistry, Tellurium chemistry, Concanavalin A chemistry, Canavalia chemistry, Biocompatible Materials chemistry, Microscopy, Fluorescence, Quantum Dots chemistry, Leptospira
- Abstract
Leptospirosis is a global public health problem caused by Gram-negative pathogenic bacteria belonging to the genus Leptospira . The disease is transmitted through the urine of infected animals, which contaminates water and soil, leading to the infection of other animals and humans. Currently, several approaches exist to detect these bacteria; however, a new sensitive method for the live-cell imaging of Leptospira is required. In this study, we report the green synthesis of cadmium telluride quantum dots (CdTe QDs) which are unique fluorescent nanocrystals with a high fluorescence quantum yield capable of modifying cell surfaces and are biocompatible with cells. The fabrication of QDs with concanavalin A (ConA), a carbohydrate-binding lectin and known biological probe for Gram-negative bacteria, produced ConA-QDs which can effectively bind on Leptospira and exhibit strong fluorescence under simple fluorescence microscopy, allowing the live-cell imaging of the bacteria. Overall, we performed the simple synthesis of ConA-QDs and demonstrated their potential use as versatile fluorescent probes for the live-cell imaging of Leptospira . This technique could be further applied to track leptospiral cells and study the infection mechanism, contributing to a more thorough understanding of leptospirosis and how to control it in the future.
- Published
- 2024
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6. Human Vγ9Vδ2 T cell expansion and their cytotoxic responses against cholangiocarcinoma.
- Author
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Sawaisorn P, Gaballa A, Saimuang K, Leepiyasakulchai C, Lertjuthaporn S, Hongeng S, Uhlin M, and Jangpatarapongsa K
- Subjects
- Humans, Interleukin-15 pharmacology, Interleukin-18, Leukocytes, Mononuclear metabolism, Receptors, Antigen, T-Cell, gamma-delta metabolism, T-Lymphocytes, Lymphocyte Activation, Antineoplastic Agents, Cholangiocarcinoma
- Abstract
Human Vγ9Vδ2 T lymphocytes are regarded as promising effector cells for cancer immunotherapy since they have the ability to eliminate several tumor cells through non-peptide antigen recognition. However, the cytotoxic function and the mechanism of Vγ9Vδ2 T cells leading to specific killing of cholangiocarcinoma cells are yet to be confirmed. In this study, we established a protocol for ex vivo expansion of Vγ9Vδ2 T cells from healthy donors' peripheral blood mononuclear cells by culture with zoledronate and addition of IL-2, and IL-15 or IL-18 or neither. Testing the cytotoxic capacity of cultured Vγ9Vδ2 T cells against cholangiocarcinoma cell lines showed higher reactivity than against control cells. Surface expression of CD107 was detected on the Vγ9Vδ2 T cells, suggesting that these cells limit in vitro growth of cholangiocarcinoma cells via degranulation of the perforin and granzyme pathway. Analysis of molecular signaling was used to demonstrate expression of pro- and anti-survival genes and a panel of cytokine genes in Vγ9Vδ2 T cells. We found that in the presence of either IL-15 or IL-18, levels of caspase 3 were significantly reduced. Also, IL-15 and IL-18 stimulated cells contained cytotoxicity against cholangiocarcinoma cells, suggesting that stimulated Vγ9Vδ2 T cells may provide a feasible therapy for cholangiocarcinoma., (© 2024. The Author(s).)
- Published
- 2024
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7. Enhancing leptospirosis control with nanosensing technology: A critical analysis.
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Suwannin P, Jangpatarapongsa K, Polpanich D, Alhibshi A, Errachid A, and Elaissari A
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- Humans, Animals, Latex Fixation Tests veterinary, Leptospirosis diagnosis, Leptospirosis prevention & control, Leptospirosis microbiology, Leptospirosis veterinary, Leptospira, Nanoparticles
- Abstract
Leptospirosis is a serious health problem in tropical areas; thus, animals shed leptospires in the environment. Humans are accidental hosts infected through exposure to contaminating bacteria in the environment. One health strategy can be applied to protect and eliminate leptospirosis because this cooperates and coordinates activities between doctors, veterinarians, and ecologists. However, conventional methods still have limitations. Therefore, the main challenges of leptospirosis control are the high sensing of detection methods to screen and control the pathogens. Interestingly, nano sensing combined with a leptospirosis detection approach can increase the sensitivity and eliminate some limitations. This article reviews nanomaterial development for an advanced leptospirosis detection method, e.g., latex beads-based agglutination test, magnetic nanoparticles enrichment, and gold-nanoparticles-based immunochromatographic assay. Thus, nanomaterials can be functionalized with biomolecules or sensing molecules utilized in various mechanisms such as biosensors. Over the last decade, many biosensors have been developed for Leptospira spp. pathogen and others. The evolution of biosensors for leptospirosis detection was designed for high efficiency and might be an alternative tool. In addition, the high-sensing fabrications are useful for leptospires screening in very low levels, for example, soil or water from the environment., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)
- Published
- 2024
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8. Solvent-sensitive nanoparticle-enhanced PCR assay for the detection of enterotoxigenic Escherichia coli.
- Author
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Teawprasong P, Wongngam Y, Tangchaikeeree T, Elaissari A, Tangboriboonrat P, Polpanich D, and Jangpatarapongsa K
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- Solvents, Polymerase Chain Reaction, Polymers, Enterotoxigenic Escherichia coli genetics, Nanoparticles
- Abstract
Stimulus-responsive nanoparticles are among the most utilized nanoscale materials in biomedical applications. As these nanoparticles exhibit a manipulable response to a particular stimulus, such as pH, heat, and organic solvent, they are potential signalling units in diagnostic assays. This study aims to enhance the limit of detection and reduce the turnaround time of magnetic nanoparticle polymerase chain reaction (PCR) enzyme-linked gene assay (MELGA), an advanced PCR-based technique termed the solvent-sensitive nanoparticle (SSNP)-enhanced PCR assay. This technique was proposed to detect pathogenic enterotoxigenic Escherichia coli (ETEC) through applying stimulus-responsive nanoparticles. The SSNPs were elaborated with three main components, including mesoporous silica nanoparticles as a structural unit, organic dye (Nile red) as a payload, and the corresponding organic solvent-sensitive polymer shell as "gatekeeper" (poly(maleic anhydride-alt-methyl vinyl ether, PMAMVE). A suitable organic solvent capable of inducing polymer swelling and dye dissolution was investigated by considering a solubility parameter. Using ethanol, the encapsulated Nile red can diffuse out of the SSNPs faster than other solvents and reach a constant concentration within 15 min. For the PCR inhibition study, various SSNPs concentrations (10-30 μg/reaction) were mixed with the ETEC gene and PCR reagent. The results showed that the particles in this concentration range did not inhibit PCR. By comparing the efficacy of conventional PCR, MELGA, and SSNP-enhanced PCR assay, the proposed technique showed a better detection limit than that of PCR, whereas that of MELGA was the lowest. Moreover, compared to MELGA or conventional PCR, this technique provided remarkably faster results in the postamplification process., (© 2022. The Author(s).)
- Published
- 2022
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9. A deep learning model (FociRad) for automated detection of γ-H2AX foci and radiation dose estimation.
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Wanotayan R, Chousangsuntorn K, Petisiwaveth P, Anuttra T, Lertchanyaphan W, Jaikuna T, Jangpatarapongsa K, Uttayarat P, Tongloy T, Chousangsuntorn C, and Boonsang S
- Subjects
- DNA Breaks, Double-Stranded, Microscopy, Confocal, Radiation Dosage, X-Rays, Deep Learning
- Abstract
DNA double-strand breaks (DSBs) are the most lethal form of damage to cells from irradiation. γ-H2AX (phosphorylated form of H2AX histone variant) has become one of the most reliable and sensitive biomarkers of DNA DSBs. However, the γ-H2AX foci assay still has limitations in the time consumed for manual scoring and possible variability between scorers. This study proposed a novel automated foci scoring method using a deep convolutional neural network based on a You-Only-Look-Once (YOLO) algorithm to quantify γ-H2AX foci in peripheral blood samples. FociRad, a two-stage deep learning approach, consisted of mononuclear cell (MNC) and γ-H2AX foci detections. Whole blood samples were irradiated with X-rays from a 6 MV linear accelerator at 1, 2, 4 or 6 Gy. Images were captured using confocal microscopy. Then, dose-response calibration curves were established and implemented with unseen dataset. The results of the FociRad model were comparable with manual scoring. MNC detection yielded 96.6% accuracy, 96.7% sensitivity and 96.5% specificity. γ-H2AX foci detection showed very good F1 scores (> 0.9). Implementation of calibration curve in the range of 0-4 Gy gave mean absolute difference of estimated doses less than 1 Gy compared to actual doses. In addition, the evaluation times of FociRad were very short (< 0.5 min per 100 images), while the time for manual scoring increased with the number of foci. In conclusion, FociRad was the first automated foci scoring method to use a YOLO algorithm with high detection performance and fast evaluation time, which opens the door for large-scale applications in radiation triage., (© 2022. The Author(s).)
- Published
- 2022
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10. Quantification of histone H2AX phosphorylation in white blood cells induced by ex vivo gamma irradiation of whole blood by both flow cytometry and foci counting as a dose estimation in rapid triage.
- Author
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Wanotayan R, Wongsanit S, Boonsirichai K, Sukapirom K, Buppaungkul S, Charoenphun P, Songprakhon P, Jangpatarapongsa K, and Uttayarat P
- Subjects
- Dose-Response Relationship, Radiation, Flow Cytometry, Humans, Leukocytes metabolism, Lymphocytes metabolism, Phosphorylation radiation effects, Histones metabolism, Triage methods
- Abstract
A quick, reliable, and reproducible biological assay to distinguish individuals with possible life-threatening risk following radiological or nuclear incidents remains a quest in biodosimetry. In this paper, we examined the use of a γ-H2AX assay as an early dose estimation for rapid triage based on both flow cytometry and image analyses. In the experiment, whole blood from 11 donors was irradiated ex vivo inside a water phantom by gamma rays from Co-60 at 0.51 Gy/min. After the lysis of red blood cells, the white blood cells were collected for immunofluorescence labeling of γ-H2AX, CD45, and nuclear stained for signal collection and visualization. Analysis by flow cytometry showed that the relative γ-H2AX intensities of lymphocytes and granulocytes increased linearly with absorbed doses from 0 to 6 Gy with a large variation among individuals observed above 2 Gy. The relative γ-H2AX intensities of lymphocytes assessed by two different laboratories were highly correlated (ICC = 0.979). Using confocal microscopic images, γ-H2AX foci were observed to be discretely distributed inside the nuclei and to increase proportionally with doses from 0 to 2 Gy, whereas large plagues of merged foci appeared at 4 and 6 Gy, resulting in the saturation of foci counts above 4 Gy. The number of total foci per cell as well as the number of foci per plane were significantly different at 0 vs 1 and 2 vs 4 Gy doses (p < 0.01). Blind tests at 0.5 Gy and 1 Gy doses showed that dose estimation by flow cytometry had a mean absolute difference of less than 0.5 Gy from the actual value. In conclusion, while flow cytometry can provide a dose estimation with an uncertainty of 0.5 Gy at doses ≤ 1 Gy, foci counting can identify merged foci that are prominent at doses ≥ 4 Gy., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2022
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11. Automated segmentation of lung, liver, and liver tumors from Tc-99m MAA SPECT/CT images for Y-90 radioembolization using convolutional neural networks.
- Author
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Chaichana A, Frey EC, Teyateeti A, Rhoongsittichai K, Tocharoenchai C, Pusuwan P, and Jangpatarapongsa K
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- Humans, Lung, Microspheres, Neural Networks, Computer, Retrospective Studies, Single Photon Emission Computed Tomography Computed Tomography, Yttrium Radioisotopes therapeutic use, Embolization, Therapeutic, Liver Neoplasms diagnostic imaging, Liver Neoplasms radiotherapy
- Abstract
Purpose:
90 Y selective internal radiation therapy (SIRT) has become a safe and effective treatment option for liver cancer. However, segmentation of target and organ-at-risks is labor-intensive and time-consuming in90 Y SIRT planning. In this study, we developed a convolutional neural network (CNN)-based method for automated lungs, liver, and tumor segmentation on99m Tc-MAA SPECT/CT images for90 Y SIRT planning., Methods:99m Tc-MAA SPECT/CT images and corresponding clinical segmentations were retrospectively collected from 56 patients who underwent90 Y SIRT. The collected data were used to train three CNN-based segmentation algorithms for lungs, liver, and tumor segmentation. Segmentation performance was evaluated using the Dice similarity coefficient (DSC), surface DSC, and average symmetric surface distance (ASSD). Dosimetric parameters (volume, counts, and lung shunt fraction) were measured from the segmentation results and were compared with clinical reference segmentations., Results: The evaluation results show that the method can accurately segment lungs, liver, and tumor with median [interquartile range] DSCs of 0.98 [0.97-0.98], 0.91 [0.83-0.93], and 0.85 [0.71-0.88]; surface DSCs of 0.99 [0.97-0.99], 0.86 [0.77-0.93], and 0.85 [0.62-0.93], and ASSDs of 0.91 [0.69-1.5], 4.8 [2.6-8.4], and 4.7 [3.5-9.2] mm, respectively. Dosimetric parameters from the three segmentation networks show relationship with those from the reference segmentations. The overall segmentation took about 1 min per patient on an NVIDIA RTX-2080Ti GPU., Conclusion: This work presents CNN-based algorithms to segment lungs, liver, and tumor from99m Tc-MAA SPECT/CT images. The results demonstrated the potential of the proposed CNN-based segmentation method for assisting90 Y SIRT planning while drastically reducing operator time., (© 2021 The Authors. Medical Physics published by Wiley Periodicals LLC on behalf of American Association of Physicists in Medicine.)- Published
- 2021
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12. Increased sensitivity of enterotoxigenic Escherichia coli detection in stool samples using oligonucleotide immobilized-magnetic nanoparticles.
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Jangpatarapongsa K, Saimuang K, Polpanich D, Thiramanas R, Techakasikornpanich M, Yudech P, Paripurana V, Leepiyasakulchai C, and Tangboriboonrat P
- Abstract
PCR detection of enterotoxigenic Escherichia-coli (ETEC) can be used directly on stool sample. However, it still has limitations due to presence of PCR inhibitors and interferences. This study, oligonucleotide primer specific to ETEC was immobilized onto MNPs and applied for separation and enrichment of ETEC-DNA from contaminants in stool after boiling. DNA separation efficiency was evaluated using conventional PCR and magneto-PCR-enzyme linked-gene-assay (MELGA). Due to high specificity of primer and efficiency of nanoparticles to bring down PCR inhibitors, DNA separation using primer-immobilized-MNPs exhibited 100-fold increase of sensitivity compared to that using simple boiling. Moreover, the sensitivities in stool were increased from 10
8 to 106 CFU/mL and 104 to 102 CFU/mL when PCR products were detected by gel electrophoresis and MELGA, respectively. Results suggested that oligonucleotide-immobilized-MNPs combined with boiling DNA extraction method was successfully used to separate the DNA of ETEC in stool with high sensitivity using MELGA., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2021 The Authors.)- Published
- 2021
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13. Heat-enhancing aggregation of gold nanoparticles combined with loop-mediated isothermal amplification (HAG-LAMP) for Plasmodium falciparum detection.
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Suwannin P, Polpanich D, Leelayoova S, Mungthin M, Tangboriboonrat P, Elaissari A, Jangpatarapongsa K, Ruang-Areerate T, and Tangchaikeeree T
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- Gold, Hot Temperature, Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques, Sensitivity and Specificity, Metal Nanoparticles, Plasmodium falciparum genetics
- Abstract
Malaria infection represents a major public health and economic issue that leads to morbidity and mortality globally. A highly effective and uncomplicated detection tool is required for malaria control in geographical hotspots of transmission. We developed a simple and more sensitive novel approach for the detection of the 18S rRNA gene of Plasmodium falciparum based on loop-mediated isothermal amplification (LAMP) and visualization using colorimetric, streptavidin-functionalized gold nanoparticles (SA-GNPs). Two loop primers of LAMP were biotinylated to produce biotin-containing products during amplification. After the addition of SA-GNPs, clusters of avidin-biotin complexes were established in the LAMP structure. While the positive reactions remained wine red, the negative reactions became colorless with partial aggregations induced by hydrochloric acid (HCl) under heat enhancement (60 °C). All steps of the assay were completed within 50 min, its detection limit was 1 parasite/μL, and it was highly specific for P. falciparum. This effortless detection system with high sensitivity and specificity could provide an alternative choice for malaria diagnostics in resource-limited regions., Competing Interests: Declaration of Competing Interest The authors report no declarations of interest., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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14. A model of modified meta -iodobenzylguanidine conjugated gold nanoparticles for neuroblastoma treatment.
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Saimuang K, Suttisintong K, Kaewchangwat N, Thanayupong E, Wongngam Y, Charoenphun P, Wanotayan R, Elaissari A, Hongeng S, Polpanich D, and Jangpatarapongsa K
- Abstract
Iodine-131 meta -iodobenzylguanidine (
131 I- m IBG) has been utilized as a standard treatment to minimize adverse side effects by targeting therapies to bind to the norepinephrine transporter (NET) expressed on 90% of neuroblastoma cells. However, only a minority of patients who receive131 I- m IBG radiotherapy have clinical responses, and these are usually not curative. In this study, novel ligand-conjugated gold nanoparticles (GNPs) based on m IBG were synthesized and evaluated biologically with neuroblastoma cells in vitro . To induce specific internalization to the tumor cells and utilize it as a model for radioenhancement,127 I-modified m IBG was successfully synthesized and grafted covalently to the surface of carboxylated PEG-GNPs. 49.28% of the novel m IBG derivative was grafted on carboxylated PEG-GNPs. The particles were stable and not toxic to the normal fibroblast cell line, L929, even at the highest concentration tested (1013 NPs per mL) at 24, 48, and 72 h. Moreover, the cellular uptake of the model was decreased significantly in the presence of a NET inhibitor, suggesting that there was specific internalization into neuroblastoma cells line (SH-SY5Y) via the NET. Therefore, this model provides useful guidance toward the design of gold nanomaterials to enhance the efficiency of131 I- m IBG treatment in neuroblastoma patients. However, the investigation of radio-therapeutic efficiency after radioisotope131 I substitution will be further conducted in a radiation safety laboratory using an animal model., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)- Published
- 2021
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15. Development of loop-mediated isothermal amplification (LAMP) assay using SYBR safe and gold-nanoparticle probe for detection of Leishmania in HIV patients.
- Author
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Ruang-Areerate T, Sukphattanaudomchoke C, Thita T, Leelayoova S, Piyaraj P, Mungthin M, Suwannin P, Polpanich D, Tangchaikeeree T, Jangpatarapongsa K, Choowongkomon K, and Siripattanapipong S
- Subjects
- Adolescent, Colorimetry, DNA, Protozoan genetics, DNA, Protozoan metabolism, HIV Infections virology, Humans, Leishmaniasis etiology, Leishmaniasis pathology, Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques, DNA, Protozoan analysis, Gold chemistry, HIV isolation & purification, HIV Infections complications, Leishmania isolation & purification, Leishmaniasis diagnosis, Metal Nanoparticles chemistry
- Abstract
Asymptomatic leishmaniasis cases have continuously increased, especially among patients with HIV who are at risk to develop further symptoms of cutaneous and visceral leishmaniasis. Thus, early diagnosis using a simple, sensitive and reliable diagnostic assay is important because populations at risk mostly reside in rural communities where laboratory equipment is limited. In this study, the highly sensitive and selective determination of Leishmania infection in asymptomatic HIV patients was achieved using dual indicators (SYBR safe and gold-nanoparticle probe; AuNP-probe) in one-step LAMP method based on basic instruments. The assay can be simply evaluated under the naked eye due to clear interpretation of fluorescent emission of LAMP-SYBR safe dye-complex and colorimetric precipitate of specific AuNP-probes. The sensitivities and specificities of fluorescent SYBR safe dye and AuNP-probe indicators were equal, which were as high as 94.1 and 97.1%, respectively. Additionally, detection limits were 10
2 parasites/mL (0.0147 ng/µL), ten times more sensitivity than other related studies. To empower leishmaniasis surveillance, this inexpensive one-step SYBR safe and AuNP-LAMP assay is reliably fast and simple for field diagnostics to point-of-care settings, which can be set up in all levels of health care facilities including resource limited areas, especially in low to middle income countries.- Published
- 2021
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16. Near-infrared polyfluorene encapsulated in poly(ε-caprolactone) nanoparticles with remarkable large Stokes shift.
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Joothamongkon J, Asawapirom U, Thiramanas R, Jangpatarapongsa K, and Polpanich D
- Abstract
Near-infrared (NIR) fluorescent dyes have attracted increasing attention as fluorescent probes in biomedical applications due to their low biological autofluorescence as well as high tissue penetration depth. However, their being hydrophobic in nature limits their clinical use as they are prone to aggregate in the physiological environment. Herein, we have designed and synthesized a novel polymeric NIR fluorescent dye and then encapsulated it into a poly(ε-caprolactone) (PCL) matrix by way of an emulsion-diffusion technique. The effect of the structure of the surfactant on the nanoparticle properties is investigated. Results show that polymeric surfactant, Kolliphor® P188, allows the formation of a high fluorescence intensity of the nanoparticles with the highest level homogeneity and stability. The synthesized nanoparticles show significant advantages in terms of a remarkable large stokes shift (276 nm) in the aqueous solution and excellent biocompatibility. The fabrication process is not limited to encapsulation of polymeric fluorescent dye. The synthesized NIR polymeric nanoparticles would be potentially applicable for biomedical applications., Competing Interests: The authors declare no conflict of interest., (This journal is © The Royal Society of Chemistry.)
- Published
- 2020
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17. Fabrication of functional hollow magnetic polymeric nanoparticles with controllable magnetic location.
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Wichaita W, Polpanich D, Kaewsaneha C, Jangpatarapongsa K, and Tangboriboonrat P
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- HeLa Cells, Humans, Magnetic Phenomena, Optical Imaging, Particle Size, Polymers chemical synthesis, Porosity, Surface Properties, Magnetite Nanoparticles chemistry, Polymers chemistry
- Abstract
Hollow magnetic polymeric particles (HoMPs) with controllable location of magnetic nanoparticles and functionality of polymeric double shell were fabricated by means of the facile soft templating method in one-pot. During the in situ miniemulsion polymerization, hexadecane, the Ostwald suppressing agent, acted as a soft template, which later formed a controllable void size. Adjusting ratio and polarity of monomers caused the difference in polymerization kinetics and phase separation, which resulted in HoMPs with two internal architectures, i.e., HoMPs-I with magnetic at the inner shell/void interface and HoMPs-II with magnetic-embedded shell. Both HoMPs-I and II contained high magnetic content (30-50%wt) with sufficient saturation magnetization (16-30 emu/g). With large void volume (>50%) and distinct shell polarity, either hydrophilic Rhodamine B or hydrophobic fluorescein isothiocyanate dye was selectively loaded. After functionalization with a cancer cell targeting ligand, folic acid (FA), the cellular uptake of HoMPs-FA in HeLa cell was improved with 100% cell viability and without cell destruction. This fabrication method provides a facile mean to tailor surface chemistry and architectures of hollow hybrid particles, which would be potentially used for efficient treatment of cancer tumors., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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18. PMMA particles coated with chitosan-silver nanoparticles as a dual antibacterial modifier for natural rubber latex films.
- Author
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Suteewong T, Wongpreecha J, Polpanich D, Jangpatarapongsa K, Kaewsaneha C, and Tangboriboonrat P
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- Animals, Anti-Bacterial Agents chemistry, Cell Proliferation drug effects, Cells, Cultured, Escherichia coli drug effects, Fibroblasts cytology, Fibroblasts drug effects, Metal Nanoparticles chemistry, Mice, Staphylococcus aureus drug effects, Surface Properties, Anti-Bacterial Agents pharmacology, Chitosan chemistry, Latex chemistry, Metal Nanoparticles administration & dosage, Polymethyl Methacrylate chemistry, Rubber chemistry, Silver chemistry
- Abstract
The antibacterial activity in sulphur prevulcanized natural rubber (SPNR) latex film was effectively improved by deposition of poly(methyl methacrylate) (PMMA) particles encircled with chitosan-coated silver nanoparticles (AgNPs-CS). With the focus on a green process, CS was selected as a safe reducing and stabilizing agent for the one-step synthesis of AgNPs-CS (38 nm, +40.4 mV) in an autoclave. The adsorption of small-sized AgNPs-CS directly onto rubber film did not provide an inhibitory effect on S. aureus. It also had a low antibacterial effect on E. coli. This is because of the particles becoming completely/partially submerged into the soft rubber matrix upon drying. Hence, the AgNPs-CS were fabricated as a shell surrounding a rigid PMMA core (496 nm, -30.9 mV). This was done using a heterocoagulation technique prior to coating on SPNR film. The presence of PMMA/AgNPs-CS on the surface of SPNR film effectively increased the surface roughness from ca. 44 to 150 nm. This substantially promoted the antibacterial activity against E. coli and S. aureus by way of contact killing and repelling mechanisms. The cytotoxicity on L-929 fibroblasts was also suppressed. This study would be, therefore, applicable to the development of antibacterial SPNR film with high surface roughness, low cytotoxicity. It could also be applied for other soft substrates., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2019
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19. Antigen-Presenting Cell Characteristics of Human γδ T Lymphocytes in Chronic Myeloid Leukemia.
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Sawaisorn P, Tangchaikeeree T, Chan-On W, Leepiyasakulchai C, Udomsangpetch R, Hongeng S, and Jangpatarapongsa K
- Subjects
- Antigen Presentation, Antigen-Presenting Cells transplantation, Antigens, Neoplasm immunology, Cell Differentiation, Cell Line, Humans, Leukemia, Myelogenous, Chronic, BCR-ABL Positive immunology, Leukocytes, Mononuclear, Lymphocyte Activation, Receptors, Antigen, T-Cell, gamma-delta metabolism, T-Lymphocytes transplantation, Antigen-Presenting Cells immunology, Cancer Vaccines immunology, Immunotherapy, Adoptive methods, Leukemia, Myelogenous, Chronic, BCR-ABL Positive therapy, T-Lymphocytes immunology
- Abstract
Human γδ T lymphocytes play a role in the immune system defense against cancer. Their broad anti-cancer activity against different types of cancers makes them outstanding candidates for cancer immunotherapy. An issue of recent interest is whether their antigen presentation features are similar to mature dendritic cells. The antigen-presenting cell (APC)-like phenotype and function of γδ T lymphocytes have been confirmed in many clinical trials. In this study, to support the strong role played by Vγ9Vδ2 T cells against cancer, we provide evidence that Vγ9Vδ2 T cells activated with chronic myeloid leukemia (CML) cell lysate antigens can efficiently express an APC phenotype and function. Vγ9Vδ2 T cells derived from normal peripheral blood mononuclear cells were activated with tumor cell lysate, and the tumor-activated Vγ9Vδ2 T cells could recognize and kill CML through their cytotoxic activity. In conclusion, the Vγ9Vδ2 T cells activated by cancer cell lysate showed APC characteristics, and this may greatly increase interest in investigating their therapeutic potential in hematologic malignancies. Abbreviations: CML: chronic myeloid leukemia; APC: antigen-presenting cell; TCR: T cell receptor; MHC: major histocompatibility complex; N-BPs: nitrogen-containing bisphosphonates; IPP: isopentenyl pyrophosphate; PBMC: peripheral blood mononuclear cells; NKG2D: natural killer receptor group 2, member D; TRAIL: tumor necrosis factor-related apoptosis-inducing ligand.
- Published
- 2019
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20. Sensitive detection of the IS 6110 sequence of Mycobacterium tuberculosis complex based on PCR-magnetic bead ELISA.
- Author
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Kyaw SP, Hanthamrongwit J, Jangpatarapongsa K, Khaenam P, and Leepiyasakulchai C
- Abstract
Tuberculosis (TB) is ranked as the top killer among infectious diseases worldwide. Early and accurate diagnosis of the disease is crucial to end the global TB epidemic. The current commercially available molecular tests are still unaffordable by most TB affected communities. Herein, we developed a novel rapid and sensitive diagnostic method to detect the IS 6110 sequence of Mycobacterium tuberculosis ( M. tuberculosis ) complex using PCR-magnetic bead ELISA. PCR amplification ofa 123 bp repetitive sequence of the IS 6110 gene was performed by using digoxigenin (DIG) and biotin-labelled primers. Streptavidin-conjugated magnetic beads were used to collect the dual-labelled amplicons and subsequently, colourimetric detection was done by using horseradish peroxidase (HRP)-conjugated anti-DIG antibody. This method is able to detect M. tuberculosis DNA down to 0.5 fg per reaction within 3 hours. The sensitivity of IS 6110 PCR detection by magnetic bead ELISA is 100 times higher than that of conventional agarose gel electrophoresis. The assay specificity was determined using a panel of DNA extracted from 10 common bacteria causing lower respiratory tract infections. No cross-reactivity was detected from those bacteria by IS 6110 PCR-magnetic bead ELISA. Thus, the novel highly sensitive and specific, reduced assay time and simplicity of this PCR-magnetic bead ELISA for the detection of the specific gene of M. tuberculosis complex makes it an attractive diagnostic tool for large-scale screening of tuberculosis in standard clinical laboratories., Competing Interests: The authors have no conflicts of interest to declare., (This journal is © The Royal Society of Chemistry.)
- Published
- 2018
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21. Enrichment of human Vγ9Vδ2 T lymphocytes by magnetic poly(divinylbenzene- co -glycidyl methacrylate) colloidal particles conjugated with specific antibody.
- Author
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Sawaisorn P, Tangchaikeeree T, Polpanich D, Midoeng P, Udomsangpetch R, Elaissari A, and Jangpatarapongsa K
- Abstract
γδ T cells play a significant role in protection against cancer. Purification of γδ T cells is needed for insight when studying their anti-cancer functionality and their utilization in adoptive cell therapy. To improve the purification of γδ T cells, in this work, a composite material based on magnetic nanoparticles was developed for purification of Vγ9Vδ2 T cells, the predominant subset of γδ T lymphocytes in human peripheral blood. The epoxy-functionalized magnetic poly(divinylbenzene- co -glycidyl methacrylate) particles (mPDGs) were bio-conjugated with anti-human Vδ2 antibody to provide specific recognition sites for T cell receptors of Vγ9Vδ2 T cells. Using fluorescence-activated cell sorting (FACS) analysis, separation of Vγ9Vδ2 T cells from peripheral blood mononuclear cells of healthy donors was confirmed with high purity [89.77% (range 87.00-91.80, n = 3)]. More interestingly, the immobilized particles did not affect the viability of purified cells as high cell viability was indicated (>90%). By combining the properties of magnetic nanoparticles with specific antibodies, these immobilized particles were shown to be used as a cell-friendly purification tool of Vγ9Vδ2 T lymphocytes without any limits for the further use of cells. The purified Vγ9Vδ2 T cells using the antibody-immobilized epoxy-functionalized mPDGs could be used directly without a detachment step for further cultivation and expansion. This highlights the advantages of this method in allowing the study of cell function and further investigation of such rare T cell populations in immunotherapy., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)
- Published
- 2018
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22. Inhibitory effect of oxidative damage on cardiomyocyte differentiation from Wharton's jelly-derived mesenchymal stem cells.
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Nimsanor N, Phetfong J, Plabplueng C, Jangpatarapongsa K, Prachayasittikul V, and Supokawej A
- Abstract
Ischemic heart diseases are a serious health problem worldwide. The transplantation of mesenchymal stem cells (MSCs) has been investigated in numerous clinical trials on various other diseases due to the self-renewal capacity of these cells and their potential to differentiate into a variety of cell types. The presence of excess reactive oxygen species in injured myocardium causes cardiac dysfunction and leads to inefficient repair of the heart. The poor outcomes of stem cell transplantation have been suggested to result from residual oxidative damage affecting the transplanted cells. The aim of the present study was to compare the effects of hydrogen peroxide (H
2 O2 ) on Wharton's jelly-derived MSCs (WJ-MSCs) and bone marrow-derived MSCs (BM-MSCs) in vitro , in order to provide information useful for the future selection of MSC types for cardiac differentiation and transplantation. H2 O2 at concentrations of 200, 500 and 1,000 µM was applied to WJ-MSCs and BM-MSCs under cardiogenic differentiation conditions. The morphology of MSCs treated with H2 O2 was similar to that of untreated cells, whereas the cell density decreased in direct association with the dose of H2 O2 . Cardiac differentiation markers were then evaluated by immunofluorescence analysis of GATA4 and cardiac troponin T (cTnT). The fluorescence intensity levels of the two markers were identified to be diminished by increasing doses of H2 O2 from 500 to 1,000 µM. The expression levels of homeobox protein Nkx2.5, cTnT and cardiac α-actin were also examined, and were identified to be low in the WJ-MSCs treated with 1,000 µM H2 O2 , which was similar to the findings observed in BM-MSCs. These results suggested that oxidative stress affects cardiomyocyte differentiation via the downregulation of cardiac genes and cardiac proteins. Furthermore, it should be noted that there was a marked difference in the effect depending on the source of MSCs. This evidence provided supportive information for the use of stem cells in transplantation.- Published
- 2017
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23. Combination of PCR and dual nanoparticles for detection of Plasmodium falciparum.
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Tangchaikeeree T, Polpanich D, Bentaher A, Baraket A, Errachid A, Agusti G, Elaissari A, and Jangpatarapongsa K
- Subjects
- Nucleic Acids chemistry, Plasmodium falciparum, Nanoparticles chemistry, Polymerase Chain Reaction methods, Polymers chemistry
- Abstract
Highly reactive particle-based DNA amplification was developed for the detection of the Pfg377 gene from P. falciparum gametocytes using functional magnetic latex particles (MLPs) and quantum dots encapsulated polymer particles (QDs-PPs). Firstly, MLPs were prepared from the precipitation of iron oxide, polymerization using initiator, and adsorption of aminodextran (AMD) so as to provide amino-functionalized MLPs. Furthermore, amino-containing polymer particles (PPs) were prepared by emulsifier-free polymerization and encapsulated with fluorescent quantum dots (QDs) for use as a signaling support. Subsequently, poly(maleic anhydride-alt-methyl vinyl ether) (PMAMVE) copolymer was effectively used for rapid and simple grafting of amino-modified DNA primers onto the surface of amino-functionalized particles thereby providing a promising method for particle immobilization. Herein, primer-grafted particles were applied in the amplification of the Pfg377 gene using the PCR approach. After amplification, PCR products containing PMAMVE-grafted MLPs and QDs-PPs were separated using a magnet and examined via a fluorescence microscope. PMAMVE-grafted particles were not found to inhibit the PCR reaction while facilitating efficient fluorescent detection of the PCR product. Results showed high sensitivity and specificity for the detection of amplified Pfg377 gene within only a few steps. This procedure represents a novel improvement to the post-amplification analysis., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
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24. Magnetic particles for in vitro molecular diagnosis: From sample preparation to integration into microsystems.
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Tangchaikeeree T, Polpanich D, Elaissari A, and Jangpatarapongsa K
- Subjects
- Lab-On-A-Chip Devices, Magnetics methods, Microfluidic Analytical Techniques methods, Biosensing Techniques methods
- Abstract
Colloidal magnetic particles (MPs) have been developed in association with molecular diagnosis for several decades. MPs have the great advantage of easy manipulation using a magnet. In nucleic acid detection, these particles can act as a capture support for rapid and simple biomolecule separation. The surfaces of MPs can be modified by coating with various polymer materials to provide functionalization for different applications. The use of MPs enhances the sensitivity and specificity of detection due to the specific activity on the surface of the particles. Practical applications of MPs demonstrate greater efficiency than conventional methods. Beyond traditional detection, MPs have been successfully adopted as a smart carrier in microfluidic and lab-on-a-chip biosensors. The versatility of MPs has enabled their integration into small single detection units. MPs-based biosensors can facilitate rapid and highly sensitive detection of very small amounts of a sample. In this review, the application of MPs to the detection of nucleic acids, from sample preparation to analytical readout systems, is described. State-of-the-art integrated microsystems containing microfluidic and lab-on-a-chip biosensors for the nucleic acid detection are also addressed., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
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25. Enhanced Sensitivity for Detection of Plasmodium falciparum gametocytes by magnetic nanoparticles combined with enzyme substrate system.
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Tangchaikeeree T, Sawaisorn P, Somsri S, Polpanich D, Putaporntip C, Tangboriboonrat P, Udomsangpetch R, and Jangpatarapongsa K
- Subjects
- DNA Primers genetics, Genes, Protozoan genetics, Biosensing Techniques methods, Horseradish Peroxidase metabolism, Limit of Detection, Magnets chemistry, Nanoparticles chemistry, Plasmodium falciparum cytology, Plasmodium falciparum genetics
- Abstract
The highly sensitive and specific detection of Pfg377 gene of Plasmodium falciparum gametocyte using Magnetic Nanoparticles PCR Enzyme-Linked Gene Assay (MELGA) was successfully developed. The MELGA included amplification of the Pfg377 gene by polymerase chain reaction (PCR) using magnetic nanoparticles (MNPs)-conjugated forward primer and biotinylated reverse primer, followed by post-analytical process using horseradish peroxidase (HRP)-conjugated streptavidin (SA). The complexes of MELGA product were incubated with the peroxidase substrate and hydrogen peroxide to produce the signal for colorimetric measurement. Altogether, the MELGA technique provided a highly sensitive and specific detection at 1 P. falciparum gametocyte/µL, which was more efficient than that of microscopic examination and rapid diagnostic tests (RDTs). Additionally, the MELGA could detect target gene at femtogram level, which was greater sensitive than the conventional PCR, nested PCR and loop-mediated isothermal amplification (LAMP). The MELGA technique could become a novel and practical method that overcome limitation of traditional gametocyte detection., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2017
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26. Magnetic Nanoparticles PCR Enzyme-Linked Gene Assay for Quantitative Detection of BCR/ABL Fusion Gene in Chronic Myelogenous Leukemia.
- Author
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Manthawornsiri Y, Polpanich D, Yamkamon V, Thiramanas R, Hongeng S, Rerkamnuaychoke B, Jootar S, Tangboriboonrat P, and Jangpatarapongsa K
- Subjects
- Adult, Animals, Cell Line, Tumor, Female, Fusion Proteins, bcr-abl genetics, Humans, Leukemia, Myelogenous, Chronic, BCR-ABL Positive metabolism, Male, Mice, Middle Aged, Polymerase Chain Reaction methods, RNA, Messenger metabolism, Sex Factors, Young Adult, Enzyme Assays methods, Fusion Proteins, bcr-abl metabolism, Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics, Magnetite Nanoparticles
- Abstract
Background: Magnetic nanoparticles (MNPs) have been widely used in medical diagnostic research. In this work, two technologies, MNPs and polymerase chain reaction (PCR), were combined to increase detection sensitivity and specificity. A novel technique based on the MNPs-PCR enzyme-linked gene assay (MELGA) was developed for detection of the BCR/ABL abnormal gene in chronic myelogenous leukemia (CML) patients., Methods: An MNPs-labeled BCR forward primer and a biotin-labeled ABL reverse primer were used to specifically amplify the target gene. After magnetic separation, the PCR product bound to MNPs labeled with streptavidin-conjugated horseradish peroxidase was incubated with the peroxidase substrate and hydrogen peroxide to generate the colorimetric signal., Results: When compared with real-time quantitative-PCR (RQ-PCR), the MELGA technique exhibited an increased sensitivity of <1 fg with high specificity for the BCR/ABL fusion gene in CML patients. In addition, MELGA colorimetric results correlated well with the number of copies obtained from RQ-PCR., Conclusion: This simple and cost-effective technique is suitable for monitoring CML patients during targeted therapy (tyrosine kinase inhibitors) especially in rural hospitals., (© 2015 Wiley Periodicals, Inc.)
- Published
- 2016
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27. Detection of Campylobacter DNA using magnetic nanoparticles coupled with PCR and a colorimetric end-point system.
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Jansaento W, Jangpatarapongsa K, Polpanich D, and Wonglumsom W
- Abstract
Campylobacter is an important food-borne pathogen causing acute gastroenteritis worldwide. Magnetic nanoparticle-based PCR coupled with streptavidin-horseradish peroxidase and a substrate was used for colorimetric detection. Forward primers conjugated to magnetic nanoparticles facilitated separation and concentration of Campylobacter DNA in a sample matrix. After PCR, a green color developed and was observed using the unaided eye, or detected using a spectrophotometer. High specificity and sensitivity of the 100 fg DNA/PCR reaction were achieved in pure culture experiments. The technique was applied for detection of Campylobacter on naturally contaminated chicken skin. All positive results were in agreement with results achieved using a conventional culture method. The magnetic nanoparticle-PCR-enzyme linked gene assay was practical and useful for detection of Campylobacter in complex matrices with PCR-interfering substances.
- Published
- 2016
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28. Mesenchymal stem cell in vitro labeling by hybrid fluorescent magnetic polymeric particles for application in cell tracking.
- Author
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Supokawej A, Nimsanor N, Sanvoranart T, Kaewsaneha C, Hongeng S, Tangboriboonrat P, and Jangpatarapongsa K
- Subjects
- Cell Differentiation, Cell Proliferation, Cell Survival, Cells, Cultured, Cellular Microenvironment, Chitosan chemistry, Humans, Imaging, Three-Dimensional, Microscopy, Fluorescence, Staining and Labeling methods, Wharton Jelly cytology, Cell Tracking methods, Magnetite Nanoparticles, Mesenchymal Stem Cells
- Abstract
Mesenchymal stem cells (MSCs) are a type of adult stem cell that contains multi-differentiation and proliferative properties and that shows high treatment implications for many clinical problems. The outcome of stem cell transplantation is still limited due to many factors, especially their survival and their interaction with the microenvironment after transplantation. Molecular imaging is a challenging technique that has been used to overcome this limitation and is based on the concept of labeling cells with tractable, visible, and non-toxic materials to track the cells after transplantation. In this study, magnetic polymeric nanoparticles (MPNPs) were used to directly label Wharton's jelly-derived MSCs (WJ-MSCs). After labeling, the growth rate and the viability of the MSCs as well as the time of exposure were determined. The 3D images of WJ-MSCs labeled with MPNPs for 24 h were created using confocal microscopy. The results showed that, after incubation with fluorescent MPNPs for over 8 h, the growth rate and cell viability of the WJ-MSCs was similar to those of the control. Three-dimensional imaging revealed that the fluorescent MPNPs could infiltrate into the cells and spread into the cytoplasm, which suggests that the synthesized fluorescent MPNPs could possibly label MSCs for cell tracking study and be further developed for in vivo applications.
- Published
- 2015
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29. A comparative study of natural immune responses against Plasmodium vivax C-terminal merozoite surface protein-1 (PvMSP-1) and apical membrane antigen-1 (PvAMA-1) in two endemic settings.
- Author
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Xia H, Fang Q, Jangpatarapongsa K, Zhiyong T, Cui L, Li B, and Udomsangpetch R
- Abstract
The mechanisms of cellular and humoral immune responses against P. vivax parasite remain poorly understood. Several malaria immunological studies have been conducted in endemic regions where both P. falciparum and P. vivax parasites co-exist. In this study, a comparative analysis of immunity to Plasmodium vivax antigens in different geography and incidence of Plasmodium spp. infection was performed. We characterised antibodies against two P. vivax antigens, PvMSP-1 and PvAMA-1, and the cross-reactivity between these antigens using plasma from acute malaria infected patients living in the central region of China and in the western border of Thailand. P. vivax endemicity is found in central China whereas both P. vivax and P. falciparum are endemic in Thailand. There was an increased level of anti-PvMSP-1/anti-PvAMA-1 in both populations. An elevated level of antibodies to total P. vivax proteins and low level of antibodies to total P. falciparum proteins was found in acute P. vivax infected Chinese, suggesting antibody cross-reactivity between the two species. P. vivax infected Thai patients had both anti-P. vivax and anti-P. falciparum antibodies as expected since both species are present in Thailand. More information on humoral and cell mediated immunity during acute P. vivax-infection in the area where only single P. vivax species existed is of great interest in the relation of building up anti-disease severity caused by P. falciparum. This knowledge will support vaccine development in the future.
- Published
- 2015
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30. Fluorescent chitosan functionalized magnetic polymeric nanoparticles: Cytotoxicity and in vitro evaluation of cellular uptake.
- Author
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Kaewsaneha C, Jangpatarapongsa K, Tangchaikeeree T, Polpanich D, and Tangboriboonrat P
- Subjects
- Cell Survival, Fluorescein-5-isothiocyanate chemistry, HeLa Cells, Hep G2 Cells, Humans, K562 Cells, Magnetics, Microscopy, Confocal, Microscopy, Electron, Transmission, Nanotechnology, Chitosan chemistry, Fluorescent Dyes chemistry, Nanoparticles chemistry, Polymers chemistry
- Abstract
Nanoparticles possessing magnetic and fluorescent properties were fabricated by the covalent attachment of fluorescein isothiocyanate onto magnetic polymeric nanoparticles functionalized by chitosan. The synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate were successfully used for labeling the living organ and blood-related cancer cells, i.e., HeLa, Hep G2, and K562 cells. The cytotoxicity test of nanoparticles at various incubation times indicated the high cell viability (>90%) without morphological change. The confocal microscopy revealed that they could pass through cell membrane within 2 h for K562 cells and 3 h for HeLa and Hep G2 cells and then confine inside cytoplasm of all types of tested cells for at least 24 h. Therefore, the synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate would potentially be used as cell tracking in theranostic applications., (© The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.)
- Published
- 2014
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31. Enrichment of malaria parasites by antibody immobilized magnetic nanoparticles.
- Author
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Tangchaikeeree T, Jangpatarapongsa K, Polpanich D, Thiramanas R, Pornjarone A, Udnaen S, Udomsangpetch R, and Tangboriboonrat P
- Subjects
- Cells, Cultured, Humans, Antibodies, Protozoan immunology, Erythrocytes parasitology, Immunomagnetic Separation methods, Magnetite Nanoparticles chemistry, Plasmodium falciparum immunology, Plasmodium falciparum isolation & purification
- Abstract
The simple and less expensive technique based on magnetic nanoparticles (MNPs) was developed for separation of malaria parasites containing specific antigens. The carboxylated MNPs were chemically bound with anti-P. falciparum IgG antibodies (Ab-MNPs) purified from the plasma of malaria patients and then used for removal of P. falciparum malaria-infected erythrocytes from other non-infected blood cells in malaria culture at a given percent parasitemia. The results from optical microscope showed that all blood stages parasites, i.e., ring, trophozoite and schizont, could be separated from other blood components with high purity (> or = 95%) and yield of 33.5% (the early stages of ring and trophozoite:the schizont stage were 1:1.34). Highly specific interaction between Ab-MNPs and the P. falciparum malaria infected erythrocytes was confirmed by scanning electron microscope. When compared to the centrifugation with Percoll gradient and depletion by sorbitol lysis which are specific to the mature and the ring stages, respectively, our technique would be more useful for production of high quality of parasites to use in malaria pathogenesis or immunological studies, and in detection techniques.
- Published
- 2013
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32. Reduction of cytotoxicity of natural rubber latex film by coating with PMMA-chitosan nanoparticles.
- Author
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Kanjanathaworn N, Polpanich D, Jangpatarapongsa K, and Tangboriboonrat P
- Subjects
- Animals, Cell Line, Tumor, Cell Proliferation drug effects, Hydrogen-Ion Concentration, Mice, Nanoparticles toxicity, Rubber toxicity, Surface Properties, Chitosan chemistry, Nanoparticles chemistry, Polymethyl Methacrylate chemistry, Rubber chemistry
- Abstract
Poly(methyl methacrylate) (PMMA) latex stabilized by chitosan (CS) oligomer was synthesized via the miniemulsion polymerization. By using 1% CS solution (in 0.1M acetic acid), the spherical PMMA-CS particles with an average size of 380 nm were obtained. The positive zeta potentials at pH 2-7 confirmed the presence of CS as the outermost layer of the latex particles. Therefore, these particles directly interacted with the indigenous non-rubbers at the surface of sulphur prevulcanized natural rubber (SPNR) film. The deposition of PMMA-CS particles caused an increase in surface roughness of the coated SPNR film as a function of latex concentration and immersion time. The simple coating of the rubber substrate with PMMA-CS particles effectively reduced the in vitro cytotoxicity on L-929 cells. This study would be, therefore, helpful for development of latex gloves designed for hypersensitive users., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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33. Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection.
- Author
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Thiramanas R, Jangpatarapongsa K, Asawapirom U, Tangboriboonrat P, and Polpanich D
- Subjects
- Acrylates, Humans, Latex Fixation Tests methods, Polystyrenes, Sensitivity and Specificity, Antibodies, Protozoan, Antigens, Protozoan analysis, Clinical Laboratory Techniques methods, Malaria, Falciparum diagnosis, Nanoparticles chemistry, Parasitology methods, Plasmodium falciparum isolation & purification
- Abstract
Polystyrene (PS) nanoparticle (NP) copolymerized with acrylic acid (AA) and coloured monomer, i.e. 2,3,6,7-tetra(2,2'-bithiophene)-1,4,5,8-naphthalenetetracarboxylic-N,N'-di(2-methylallyl)-bisimide (ALN8T), was synthesized via the miniemulsion polymerization. Before applying for malaria antigen detection, the blue NP was conjugated with human polyclonal malaria IgG antibody (Ab) specific to Plasmodium falciparum. For the conjugation, three methods, i.e. physical adsorption, covalent coupling and affinity binding via streptavidin (SA) and biotin interaction, were employed. The optimum ratio of Ab to NPs used in each immobilization procedure and the latex agglutination test based on the reaction between Ab conjugated NPs and malaria patient plasma were investigated. All Ab-latex conjugates provided the high sensitivity for the detection of P. falciparum malaria plasma. The highest specificity to P. falciparum was obtained from using Ab-NPs conjugated via the SA-biotin interaction., (© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.)
- Published
- 2013
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34. Detection of Vibrio cholerae using the intrinsic catalytic activity of a magnetic polymeric nanoparticle.
- Author
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Thiramanas R, Jangpatarapongsa K, Tangboriboonrat P, and Polpanich D
- Subjects
- Catalysis, Colorimetry methods, Vibrio cholerae chemistry, Magnetite Nanoparticles chemistry, Polymerase Chain Reaction methods, Polymers chemistry, Vibrio cholerae isolation & purification
- Abstract
A novel and sensitive magnetic polymeric nanoparticle (MPNP)-polymerase chain reaction-colorimetry (magneto-PCR-colorimetry) technique was developed for detection of Vibrio cholerae ( V. cholerae ). The technique involved an amplification of V. cholerae DNA on the surface of an MPNP and then employed the intrinsic catalytic activity of the MPNP to detect the target gene by colorimetry. An amino-modified forward primer was covalently labeled onto the MPNP surface which would bind to PCR product during PCR cycling. By employing the catalytic activity of the MPNP, the analysis of PCR product bound MPNP yielded a sensitivity of 10(3) CFU/mL of V. cholerae in buffer system within 4 h. The specificity and efficiency of the technique were investigated by using various bacterial DNAs in drinking and tap water.
- Published
- 2013
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35. Immunity to malaria in Plasmodium vivax infection: a study in central China.
- Author
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Jangpatarapongsa K, Xia H, Fang Q, Hu K, Yuan Y, Peng M, Gao Q, Sattabongkot J, Cui L, Li B, and Udomsangpetch R
- Subjects
- Adult, CD4-Positive T-Lymphocytes parasitology, Case-Control Studies, Cell Proliferation, China, Coculture Techniques, Cross-Sectional Studies, Flow Cytometry methods, Humans, Immune System, Interleukin-2 Receptor alpha Subunit biosynthesis, Leukocytes, Mononuclear cytology, Leukocytes, Mononuclear parasitology, Longitudinal Studies, Malaria, Vivax epidemiology, Middle Aged, Phenotype, T-Lymphocytes immunology, Malaria, Vivax immunology, Malaria, Vivax parasitology, Plasmodium vivax metabolism
- Abstract
Background: P. vivax infection is characterised by relapsing fever, indicating reinfection by previously hidden parasites in the host. Relapsed infection can lead to the activation of the memory T cell pool, which may lead to protective immunity. This study aims to characterise immune responses in acute P. vivax-infected patients living in an area of central China characterised by only P. vivax infection., Methodology/principal Findings: We conducted a cross-sectional immune-phenotypic analysis of adults using the following inclusion criteria: acute P. vivax infection (N=37), a history of P. vivax infection (N=17), and no known history of P. vivax infection (N=21). We also conducted a 2-week longitudinal analysis following acute P. vivax infection, in which PBMC proliferation was measured in response to P. vivax and P. falciparum blood stage lysates. Using flow cytometry, we showed elevated memory T cells in the blood during acute P. vivax infection. The levels of γδ T cells were two-fold higher than those measured in naive controls. This result suggested that in the two populations, memory and γδ T cells promptly responded to P. vivax parasites. Interestingly, P. falciparum antigens stimulated T cells obtained from P. vivax-infected patients during a day 14-convalescence, whereas lymphocytes from the naïve control group responded to a lower degree of convalescence., Conclusions/significance: Cell-mediated immunity during the convalescent period of the P. vivax-infected hosts was comprised of T cells that were specifically able to recognise P. falciparum antigens. Although the magnitude of the response was only half that measured after stimulation with P. vivax antigens, the matter of cross-antigenic stimulation is of great interest.
- Published
- 2012
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36. DNA detection of chronic myelogenous leukemia by magnetic nanoparticles.
- Author
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Jangpatarapongsa K, Polpanich D, Yamkamon V, Dittharot Y, Peng-On J, Thiramanas R, Hongeng S, Jootar S, Charoenmak L, and Tangboriboonrat P
- Subjects
- Cell Line, Tumor, DNA isolation & purification, Humans, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Nanoparticles ultrastructure, Sensitivity and Specificity, DNA genetics, Fusion Proteins, bcr-abl genetics, Magnetics, Nanoparticles chemistry, Polymerase Chain Reaction methods
- Abstract
A novel tool for the detection of BCR/ABL fusion gene in chronic myelogenous leukemia (CML) was developed by a magneto-polymerase chain reaction (PCR)-enzyme linked gene technique. The forward primers covalently bound to the surface of magnetic nanoparticles allowed a convenient separation of PCR products with high sensitivity (0.5 pg ml(-1)) and high specificity using K562 cell line and CML patients. The results were obtained when the biotinylated-reverse primer bound to streptavidin-horseradish peroxidase (HRP) and hydrolysed the substrate. This novel readout system was approximately 1000-fold more sensitive than the conventional agarose gel electrophoresis. The present technique is practical and useful for following up CML patients and for providing appropriate treatment, particularly to patients in remote areas.
- Published
- 2011
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37. In vitro cytotoxicity evaluation of natural rubber latex film surface coated with PMMA nanoparticles.
- Author
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Anancharungsuk W, Polpanich D, Jangpatarapongsa K, and Tangboriboonrat P
- Subjects
- Acrylic Resins chemistry, Animals, Cell Survival drug effects, Coated Materials, Biocompatible pharmacology, L Cells, Materials Testing, Mice, Microscopy, Electron, Scanning, Microspheres, Particle Size, Polymethyl Methacrylate pharmacology, Surface Properties, Coated Materials, Biocompatible chemistry, Polymethyl Methacrylate chemistry, Rubber chemistry
- Abstract
In order to increase surface roughness of the sulphur-prevulcanized natural rubber (SPNR) film and, hence, decrease the direct contact between the rubber and skin, the poly(methyl methacrylate) (PMMA) latex particles were deposited onto the SPNR film grafted with polyacrylamide (SPNR-g-PAAm). The surface coverage of PMMA particles on the SPNR-g-PAAm increased with increasing latex immersion time, particle size and concentration. Prior to the in vitro cytotoxicity evaluation on L-929 fibroblasts, the SPNR and SPNR-g-PAAm coated with PMMA particles were extracted by using the culture medium. Results showed that the cytotoxicity effect could be significantly reduced by coating PMMA particles onto the rubber film. At the extract concentrations of < or =12.5% for 24h at 37 degrees C, no toxicity potential was detected. The study will be helpful for development of gloves designed for the hypersensitive person., (2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
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38. Plasmodium vivax parasites alter the balance of myeloid and plasmacytoid dendritic cells and the induction of regulatory T cells.
- Author
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Jangpatarapongsa K, Chootong P, Sattabongkot J, Chotivanich K, Sirichaisinthop J, Tungpradabkul S, Hisaeda H, Troye-Blomberg M, Cui L, and Udomsangpetch R
- Subjects
- Adolescent, Adult, Aged, Animals, Dendritic Cells metabolism, Female, Humans, Interleukin-10 immunology, Lymphocyte Activation, Malaria, Vivax parasitology, Male, Middle Aged, T-Lymphocytes, Regulatory metabolism, Young Adult, Antigens, Protozoan immunology, Dendritic Cells immunology, Interleukin-10 metabolism, Malaria, Vivax immunology, Plasmodium vivax immunology, T-Lymphocytes, Regulatory immunology
- Abstract
Immunity induced by Plasmodium vivax infections leads to memory T-cell recruitment and activation during subsequent infections. Here, we investigated the role of regulatory T cells (Treg) in coordination with the host immune response during P. vivax infection. Our results showed a significant increase in the percentage of FOXP3+ Treg, IL-10-secreting Type I Treg (Tr1) and IL-10 levels in patients with acute P. vivax infection as compared with those found in either naïve or immune controls. The concurrent increase in the Treg population could also be reproduced in vitro using peripheral blood mononuclear cells from naïve controls stimulated with crude antigens extracted from P. vivax-infected red blood cells. Acute P. vivax infections were associated with a significant decrease in the numbers of DC, indicating a general immunosuppression during P. vivax infections. However, unlike P. falciparum infections, we found that the ratio of myeloid DC (MDC) to plasmacytoid DC (PDC) was significantly lower in acute P. vivax patients than that of naïve and immune controls. Moreover, the reduction in PDC may be partly responsible for the poor antibody responses during P. vivax infections. Taken together, these results suggest that P. vivax parasites interact with DC, which alters the MDC/PDC ratio that potentially leads to Treg activation and IL-10 release.
- Published
- 2008
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39. Memory T cells protect against Plasmodium vivax infection.
- Author
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Jangpatarapongsa K, Sirichaisinthop J, Sattabongkot J, Cui L, Montgomery SM, Looareesuwan S, Troye-Blomberg M, and Udomsangpetch R
- Subjects
- Adolescent, Adult, Aged, Animals, Antibodies, Protozoan blood, Female, Humans, Male, Middle Aged, Immunologic Memory, Malaria, Vivax immunology, T-Lymphocytes immunology
- Abstract
Immunity induced by Plasmodium vivax infection leads to memory T cell recruitment activated during "relapse" or "re-infection". This study aims to characterise memory T cells in patients with acute or convalescent P. vivax infection. Lymphocytes were collected from patients infected by P. vivax, immune controls and naive controls. The proportion of immature memory T cells, expressing CD45RO(+)CD27(+), and mature cells lacking CD27 was assessed. A statistically significant increase in the median percentage of memory T cell subsets expressing CD4(+) was observed in material from patients with an acute infection compared with that from either naive or immune controls. The high percentage of memory T cells in infected patients was maintained until 60 days post treatment. The immune controls living in a malaria endemic area had a somewhat increased proportion of memory T cell subsets expressing CD8(+). An approximately three-fold increase of these cell types was shown in patients with an acute infection and the level persisted until 60 days post treatment. Phenotypic characterisation of the peripheral lymphocytes during acute infection revealed that a large fraction of the lymphocytes carried the gammadelta phenotypes suggesting a role for these cells in the early response against P. vivax. Very low levels of P. vivax specific antibody were found. This might suggest that cell-mediated immunity may play a greater role in the development of naturally acquired protection against P. vivax infection than humoral immunity. Our results provide further insight into the mechanism of cell-mediated immunity to P. vivax infection that could be important for the future development of a successful vaccine and anti-malarial drug designation.
- Published
- 2006
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