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It is well established that the herpesvirus nuclear egress complex (NEC) has an intrinsic ability to deform membranes. During viral infection, the membrane-deformation activity of the NEC must be precisely regulated to ensure efficient nuclear egress of capsids. One viral protein known to regulate herpes simplex virus type 2 (HSV-2) NEC activity is the tegument protein pUL21. Cells infected with an HSV-2 mutant lacking pUL21 (ΔUL21) produced a slower migrating species of the viral serine/threonine kinase pUs3 that was shown to be a hyperphosphorylated form of the enzyme. Investigation of the pUs3 substrate profile in ΔUL21-infected cells revealed a prominent band with a molecular weight consistent with that of the NEC components pUL31 and pUL34. Phosphatase sensitivity and retarded mobility in phos-tag SDS-PAGE confirmed that both pUL31 and pUL34 were hyperphosphorylated by pUs3 in the absence of pUL21. To gain insight into the consequences of increased phosphorylation of NEC components, the architecture of the nuclear envelope in cells producing the HSV-2 NEC in the presence or absence of pUs3 was examined. In cells with robust NEC production, invaginations of the inner nuclear membrane were observed that contained budded vesicles of uniform size. By contrast, nuclear envelope deformations protruding outwards from the nucleus, were observed when pUs3 was included in transfections with the HSV-2 NEC. Finally, when pUL21 was included in transfections with the HSV-2 NEC and pUs3, decreased phosphorylation of NEC components was observed in comparison to transfections lacking pUL21. These results demonstrate that pUL21 influences the phosphorylation status of pUs3 and the HSV-2 NEC and that this has consequences for the architecture of the nuclear envelope.

This paper describes the development of a new geoid-based vertical datum from airborne gravity data, by the Department of Survey and Mapping Malaysia, on land and in the South China Sea out of the coast of East Malaysia region, covering an area of about 610,000 square kilometres. More than 107,000 km flight line of airborne gravity data over land and marine areas of East Malaysia has been combined to provide a seamless land-to-sea gravity field coverage; with an estimated accuracy of better than 2.0 mGal. The iMAR-IMU processed gravity anomaly data has been used during a 2014-2016 airborne survey to extend a composite gravity solution across a number of minor gaps on selected lines, using a draping technique. The geoid computations were all done with the GRAVSOFT suite of programs from DTU-Space. EGM2008 augmented with GOCE spherical harmonic model has been used to spherical harmonic degree N = 720. The gravimetric geoid first was tied at one tide-gauge (in Kota Kinabalu, KK2019) to produce a fitted geoid, my_geoid2017_fit_kk. The fitted geoid was offset from the gravimetric geoid by +0.852 m, based on the comparison at the tide-gauge benchmark KK2019. Consequently, orthometric height at the six other tide gauge stations was computed from HGPS Lev = hGPS - Nmy_geoid2017_.t_kk. Comparison of the conventional (HLev) and GPS-levelling heights (HGPS Lev) at the six tide gauge locations indicate RMS height difference of 2.6 cm. The final gravimetric geoidwas fitted to the seven tide gauge stations and is known as my_geoid2017_fit_east. The accuracy of the gravimetric geoid is estimated to be better than 5 cm across most of East Malaysia land and marine areas

Abstract Introduction This paper focused on the process of urbanization in Lahore and its correlation with the loss of urban vegetation and perceived environmental impacts based on local population’s perception through administration of a questionnaire survey. Lahore is known as a city of gardens and green spaces in Pakistan and always possessed rich green character in its long history. For the past few decades due to rapid urbanization, the city has lost its aesthetically important urban vegetation, tress, and greenery. The field studies and remote sensing data revealed that the loss of vegetation has been experienced at an astonishing rate in many union councils of the city. Methods This study was conceived to probe importance and value of the urban tress and vegetation of Lahore in an environmental context and its general importance for the residents of the city. The objectives were to analyze the role of vegetation in the life of citizens; the specific objective was to reveal the attitudes and responses of city dwellers about loss of urban trees/vegetation and its role in the overall environment, process of urbanization, and maintenance strategies. In the present paper, perception of local people (n = 2080) regarding the socio-environmental impacts due to the ongoing process of urbanization and detriments of vegetation loss has been probed through administration of a household questionnaire. The collected data has been tested with respondent’s age, gender, marital status, education, monthly income, and occupation. Results This study was an attempt to document and explore the change in historical transformation in vegetation and perception of the sample population of Lahore. This perception survey has revealed that urbanization is the major cause of loss of vegetation and has caused a number of perceived environmental problems in the city. Conclusions The study underlined an analysis of people’s perceptions regarding the loss of trees and vegetation . The perceived socio-environmental findings revealed that the urbanization and population growth are the fundamental reasons for trees/vegetation loss in the city.

In the original publication of this article (Shirazi et al., 2016) the author figured out that in the Fig. 1, the upper left corner Map of Pakistan was inappropriate as per the national policy. In map of Pakistan, Jammu & Kashmir (occupied by India) must be shown annexed with Pakistan while mentioning Azad Jammu & Kashmir as disputed territory.

The importance of the research is drawn from the fact that it treats a rhetorical concept. Alliteration is an important science for understanding the meaning of the Quranic verses, and Alliteration Derivation presents an aspect of the rhetorical miracles of the Quran as well as reveals the stylistic beauty of the Arabic language. The research aims at highlighting the beauty of the Verses containing Derivation Alliteration and what results from them such an increase in the semantic sense of the Quran. The researchers have applied in this article the inductive approach by inducing the versus that have this type of Alliteration as well as we followed the analytical method by analyzing and interpreting the verses so giving significant expressions, creativity and innovation to the Holy Quran. We have reached by the end of the research to several important results: Alliteration is one of the most important sciences to understand the rhetorical verses of the Quran and extract ideas from it. Alliteration Derivation is also not advisable unless stated in an appropriate location, and it will be bothered if it were artificial. Moreover Alliteration Derivation gives the expressions of the Quran feature innovation, creation and innovation in finding different connotations. Additionally, it is not considered only as verbal ornaments and colorful decorations that have an eloquent impact attracting the listener, making him listen to and making it easy to hear, but also it affects the psychological aspect of the human being. As a matter of fact, the alliterated form can deceive the listener, whereas it shows you that it will repeat the meaning, and echo the pronunciation. However, it really fakes the listener by giving a new sense which is completely different from the proceeding one. At that moment, one feels extremely happy and pleased.

Abstract Capnocytophaga is an opportunistic gram-negative anaerobic bacillus found in the oropharyngeal cavity of mammals and is associated with periodontal disease in humans. Sepsis, osteomyelitis, lung abscess, endocarditis, and meningitis have been reported in humans following animal bites. Perinatal infection with Capnocytophaga is infrequent and is generally considered to have a low risk of morbidity to the mother and fetus. We report a case of neonatal Capnocytophaga sepsis associated with the development of severe cystic periventricular leukomalacia.

The multifunctional tegument protein pUL21 of HSV-2 is phosphorylated in infected cells. We have identified two residues in the unstructured linker region of pUL21, serine 251 and serine 253, as phosphorylation sites. Both phosphorylation sites are absent in HSV-1 pUL21, which likely explains why phosphorylated pUL21 was not detected in cells infected with HSV-1. Cells infected with HSV-2 strain 186 viruses deficient in pUL21 phosphorylation exhibited reductions in both cell-cell spread of virus infection and virus replication. Defects in secondary envelopment of cytoplasmic nucleocapsids were also observed in cells infected with viruses deficient in pUL21 phosphorylation as well as in cells infected with multiple strains of HSV-2 and HSV-1 deleted for pUL21. These results confirm a role for HSV pUL21 in the secondary envelopment of cytoplasmic nucleocapsids and indicate that phosphorylation of HSV-2 pUL21 is required for this activity. Phosphorylation of pUL21 was substantially reduced in cells infected with HSV-2 strain 186 mutants lacking the viral serine/threonine kinase pUL13, indicating a requirement for pUL13 in pUL21 phosphorylation. IMPORTANCE It is well known that post-translational modification of proteins by phosphorylation can regulate protein function. Here, we determined that phosphorylation of the multifunctional HSV-2 tegument protein pUL21 requires the viral serine/threonine kinase pUL13. In addition, we identified serine residues within HSV-2 pUL21 that can be phosphorylated. Phenotypic analysis of mutant HSV-2 strains with deficiencies in pUL21 phosphorylation revealed reductions in both cell-cell spread of virus infection and virus replication. Deficiencies in pUL21 phosphorylation also compromised the secondary envelopment of cytoplasmic nucleocapsids, a critical final step in the maturation of all herpes virions. Unlike HSV-2 pUL21, phosphorylation of HSV-1 pUL21 was not detected. This fundamental difference between HSV-2 and HSV-1 may underlie our previous observations that the requirements for pUL21 differ between HSV species. [ABSTRACT FROM AUTHOR]

Objective Data-driven methodologies in healthcare necessitate labeled data for effective decision-making. However, medical data, particularly in unstructured formats, such as clinical notes, often lack explicit labels, making manual annotation challenging and tedious. Methods This paper introduces a novel deep active learning framework designed to facilitate the annotation process for multiclass text classification, specifically using the SOAP (subjective, objective, assessment, plan) framework, a widely recognized medical protocol. Our methodology leverages transformer-based deep learning techniques to automatically annotate clinical notes, significantly easing the manual labor involved and enhancing classification performance. Transformer-based deep learning models, with their ability to capture complex patterns in large datasets, represent a cutting-edge approach for advancing natural language processing tasks. Results We validate our approach through experiments on a diverse set of clinical notes from publicly available datasets, comprising over 426 documents. Our model demonstrates superior classification accuracy, with an F1 score improvement of 4.8% over existing methods but also provides a practical tool for healthcare professionals, potentially improving clinical documentation practices and patient care. Conclusions The research underscores the synergy between active learning and advanced deep learning, paving the way for future exploration of automatic text annotation and its implications for clinical informatics. Future studies will aim to integrate multimodal data and large language models to enhance the richness and accuracy of clinical text analysis, opening new pathways for comprehensive healthcare insights.

Rapid developments in the Internet of Things (IoT) have opened the door for game-changing applications in numerous sectors, especially the vehicle industry. There is a rising demand for efficient assessment and decision-making methodologies to pinpoint the most promising choices for the vehicle sector with the introduction of IoT-based maritime vehicles. To overcome this issue, the integrated multi-criteria decision-making analysis (MCDA) paradigm proposed in this research combines the additive ratio assessment (ARAS) and analytic hierarchy process (AHP) approaches to evaluate and choose IoT-based maritime vehicles based on their performance- and authenticity-related criteria in the vehicle sector. The selection issue is hierarchically organized, and the assessment criteria are prioritized using the AHP approach. There are seven performance and authentication related criteria are selected that might aid in the selection procedure. Using the AHP, we are assigned these criteria proportionate weights that reflect their respective significance and interrelationships. AHP, however, falls short of offering a thorough analysis of the alternatives that exist. To overcome these restrictions, this research presents the integration of AHP with the ARAS approach for the ranking of alternatives according to how well they perform against the set criteria. By using the ARAS technique, it is possible to get over the restrictions of AHP and achieve a more thorough assessment of maritime IoT-based vehicles. The efficiency of the framework is proven using empirical data and professional judgment. The findings show that the hybrid method successfully encapsulates the intricate relationships between the factors being evaluated and objectively appraises the potential of IoT-based maritime vehicles for the automotive sector. This study extends to the area by providing an organized and thorough method for assessing and choosing IoT-based maritime vehicles. Considering several factors and their mutual dependence, the hybrid AHP and ARAS technique gives decision-makers a powerful tool for evaluating the potential of IoT-based maritime vehicles in the automotive sector. Smart decisions on the deployment of IoT-based marine vehicles and maximizing the potential they present may be made by beneficiaries in the automotive sector using the study’s results.

The purpose of this research was to examine the interaction between metabolites of Lactobacillus plantarum YW11, characterized through GC–mass spectra (MS) analysis, and the FN1 protein in cases of familial pulmonary fibrosis, found from hub genes analysis. GC–MS analysis was performed to identify metabolites in L. plantarum. Then, gene expression analysis and functional annotations were conducted to investigate the hub genes. A network of hub genes and transcription factors (TFs) was constructed, highlighting the significance of FN1 in the disease’s etiology. Molecular docking was employed to explore the interaction between the characterized metabolites and the FN1 protein. Toxicity analysis was also carried out. Thirty-two active compounds of L. plantarum YW11 were characterized by GC–MS. The gene expression analysis identified 295 differentially expressed genes, including 10 hub genes and 6 TFs, providing further support for the involvement of FN1 protein in the disease. The results of the molecular docking studies suggest the therapeutic potential of targeting FN1, with the best docking result observed for the interaction between FN1 and the 2,4-di-tert-butylphenol metabolite (energy of −6.9 kcal/mol). The toxicity analysis and molecular dynamic simulations support the suitability of 2,4-di-tert-butylphenol as a candidate for targeting FN1.

Abstract Uneven rainfall and high temperature cause drought in tropical and subtropical regions which is a major challenge to cultivating summer mung bean. Potassium (K), a major essential nutrient of plants can alleviate water stress (WS) tolerance in plants. A field trial was executed under a rainout shelter with additional K fertilization including recommended K fertilizer (RKF) for relieving the harmful impact of drought in response to water use efficiency (WUE), growth, yield attributes, nutrient content, and yield of mung bean at the Regional Agricultural Research Station, BARI, Ishwardi, Pabna in two successive summer season of 2018 and 2019. Drought-tolerant genotype BMX-08010-2 (G1) and drought-susceptible cultivar BARI Mung-1 (G2) were grown by applying seven K fertilizer levels (KL) using a split-plot design with three replications, where mung bean genotypes were allotted in the main plots, and KL were assigned randomly in the sub-plots. A considerable variation was observed in the measured variables. Depending on the different applied KL and seed yield of mung bean, the water use efficiency (WUE) varied from 4.73 to 8.14 kg ha−1 mm−1. The treatment applying 125% more K with RKF (KL7) under WS gave the maximum WUE (8.14 kg ha−1 mm−1) obtaining a seed yield of 1093.60 kg ha−1. The treatment receiving only RKF under WS (KL2) provided the minimum WUE (4.73 kg ha−1 mm−1) attaining a seed yield of 825.17 kg ha−1. Results showed that various characteristics including nutrients (N, P, K, and S) content in stover and seed, total dry matter (TDM) in different growth stages, leaf area index (LAI), crop growth rate (CGR), root volume (RV), root density (RD), plant height, pod plant−1, pod length, seeds pod−1, seed weight, and seed yield in all pickings increased with increasing K levels, particularly noted with KL7. The highest grain yield (32.52%) was also obtained from KL7 compared to lower K with RKF. Overall, yield varied from 1410.37 kg ha−1 using 281 mm water (KL1; well-watered condition with RKF) to 825.17 kg ha−1 using 175 mm water (KL2). The results exhibited that the application of additional K improves the performance of all traits under WS conditions. Therefore, mung beans cultivating under WS requires additional K to diminish the negative effect of drought, and adequate use of K contributes to accomplishing sustainable productivity.

Abstract The present study was undertaken to investigate the extent of pollution load at Port Qasim coastal area, the second largest port in the country. The samples of sea water were collected from 23 locations, including the samples from Bundal Island situated in the Port Qasim area. Samples were analyzed for physico-chemical characteristics to determine the extent of pollution load. Samples were also analyzed for heavy metals including As, Cr, Cu, Cd, Pb and Ni. The investigation revealed that the Port Qasim Area is grossly polluted from inorganic and organic loads mainly coming from the nearby industrial setup. Extensively high organic load was found at the site receiving the wastewater from the Landhi Cattle colony. PQ-6 receives untreated wastewater from oil industries as exhibited by its high oil content. PQ-14 is receiving untreated industrial discharges from the nearby chemical industries and represents a very high pollution load. The sites closed to Bundal Island are relatively less polluted. The mangroves in the area are constantly under threat owing to the indiscriminate discharge of both domestic and industrial effluents. The concentration of DO although not on higher side but at least prevents the anoxic condition in the area. Out of all the heavy metals the concentration of Cr and Pb was exceptionally higher. The distribution of heavy metals demonstrated a typical diffusion pattern from the land to the sea. Principal component analysis and cluster analysis of the data showed the major gradients, similarities of localities and the variables of importance in terms of overall variability in the pollution characteristics. It is concluded that the level of pollution is increasing at an alarming rate, which is detrimental to the biodiversity of the coastal area.