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A new flow injection procedure for an assay of Fe(III) by using salicylate obtained from antipyretic powder, which is a cheap and easily available reagent, is proposed. A red complex was continuously monitored by a laboratory-made green LED colorimeter. A linear calibration was obtained in the range of 1–20 mg Fe l-1 with a detection limit of 0.5 mg Fe l-1 and R.S.D.s of 1.4–5.4% (n=3, for 1–20 mg Fe l-1). The new procedure was applied to assay iron contents in pharmaceutical preparations. The results were in good agreement with those of the USP standard method. [Copyright &y& Elsevier]

A flow injection analysis (FIA) system is described for the determination of uranium. The system consists of a microcolumn packed with. U/TEVA.SpecTM, chromatographic resin for on-line sample separation. The eluate is mixed with 4-(2-pyridylazo) resorcinol (PAR). The colored product is continuously monitored spectrophotometrically. [ABSTRACT FROM AUTHOR]

A simple gravitational field-flow fractionation (GrFFF) system was used for size separation of micron sized silica particles coated with hydrous iron oxide (geothite). The amount of iron on the particles was monitored either on-line by reverse-flow injection analysis (r-FIA) with chemiluminescence detection using luminol or off-line by electrothermal atomic absorption spectrophotometry (ETAAS). The combination of GrFFF with reverse FIA or with ETAAS has been demonstrated to be a cost-effective tool for size based iron speciation of particles. [Copyright &y& Elsevier]

A simple and low cost flow injection (FI) system with bead injection (BI) was developed for determination of low concentration (μmol l−1) of iron in water samples. Chelex-100 chelating resin beads, trapped in a jet ring cell, were employed. The intensity of red complex of 1,10-phenanthroline with Fe2+ was monitored using colorimetric detector with a LED green light source. Amount of total Fe (Fe2+ and Fe3+) and Fe2+ can be evaluated by with and without reduction of Fe3+ using ascorbic acid. Lowest detectable levels of Fe2+ were 0.90 and 0.45 μmol l−1 for sample loading time of 3 and 5 min, respectively. Working range was up to 3.90 μmol l−1 using 0.3% w/v 1, 10-phenanthroline. Percent recoveries of spiked water samples (0.90–2.33 μmol l−1 of Fe2+) were 100–110%. [Copyright &y& Elsevier]

Three flow injection (FI) systems were investigated for the determination of trace iron in beer: an FI-in-valve column-flame atomic absorption spectrophotometry (FI-FAAS) system, a spectrophotometric FI system with a column placed at the detection point, and an FI-spectrophotometric system with bead injection (FI-BI). Cationic exchange resin Dowex 50W X8 and iminodiacetate chelating resin, Chelex-100, were employed for the FI-spectrophotometric and FI-FAAS systems, respectively. The FI-in-valve column, packed with the resin, enhances the FAAS performance. The spectrophotometric FI system with a column (packed with Chelex-100) placed at the detection point (in a cell holder of a spectrophotometer) is based on the formation of iron (II)–1,10-phenanthroline complex sorbed onto the resin. No eluent has been found to be suitable. The FI-BI for renewable microcolumn has been proven to be an alternative. The FI-FAAS and FI-BI procedures provide online sample preseparation and preconcentration for the determination of iron in beer. Both are simple, rapid, and economical. The procedures also involve sample preparation (decarbonation and suppression of tannin interference by adding ascorbic acid) and standard addition. The results obtained by FI-FAAS and FI-BI agree with those of AOAC spectrophotometric method. [Copyright &y& Elsevier]

Kinetic information for the phosphate–molybdate–ascorbic acid reaction can be obtained by making use of a very simple manually operated stopped-flow injection (FI) system. Various parameters (concentrations of reagents, flow rate, mixing coils, and volume of flow cell) were investigated for determination of phosphate. A stopped-FI system should be arranged for low degree of mixing (of reactants) and low dispersion so that good signals of rate changes will be observed. Simultaneous determination of phosphate and silicate by the stopped-FI technique is proposed, using a laboratory-made semi-automatic stopped-FI Analyzer with LED-based photometer. It is based on kinetic separation of phosphate and silicate using molybdenum blue. The proposed procedure has been demonstrated for the application to water samples. The results obtained agree with that of a standard method. [Copyright &y& Elsevier]

This study describes a novel dual-mode immunosensor that combines electrochemical (EC) and surface-enhanced Raman scattering (SERS) techniques for the detection of prostate-specific antigen (PSA), a biomarker associated with prostate cancer. The sensor consists of a nanocomposite of gold nanoparticles (AuNPs) deposited on two-dimensional (2D) molybdenum disulfide (Au@MoS 2 ) modified on a working carbon electrode of a screen-printed electrode (SPE). Subsequently, the primary antibody (Ab1) is immobilized on the modified electrode, creating Ab1/Au@MoS 2 /SPE for specific recognition of the target PSA. In parallel, AuNPs are conjugated with a secondary antibody (Ab2) and a probe molecule, 3,3',5,5'-tetramethylbenzidine (TMB), leading nanotags (TMB/Ab2/AuNPs) formation exhibiting strong SERS and EC responses. Upon the presence of the target, sandwich immunocomplexes can be formed through antigen-antibody interactions (Ab1-PSA-Ab2). The differential pulse voltammetry (DPV) technique is employed for EC detection mode, while a handheld Raman spectrometer with a 785 nm excitation laser is utilized to collect SERS signals. The developed system demonstrates excellent selectivity and sensitivity, with low limits of detection (LODs) of 3.58 pg mL -1 and 4.83 pg mL -1 for EC and SERS sensing, respectively. Importantly, the dual-mode immunosensor proves effective quantifying PSA protein in human serum samples with good recovery. Given its high sensitivity and proficiency in analyzing biological samples, this proposed immunosensor holds promise as an alternative tool for the early diagnosis of cancers., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper, (Copyright © 2024 Elsevier B.V. All rights reserved.)

Metal-Organic Frameworks (MOFs) have garnered significant attention in the development of electrochemical glucose sensors due to their unique and advantageous properties. The highly tunable pore channels of MOFs facilitate optimal diffusion of glucose molecules, while their large specific surface area provides abundant active sites for electrochemical reactions. Furthermore, the well-dispersed metallic active sites within MOFs enhance electrocatalytic activity, thereby improving the sensitivity and selectivity of glucose detection. These features make MOF-based nanoarchitectures promising candidates for the development of efficient and sensitive glucose sensors, which are crucial for diabetes management and monitoring. The integration of enzymatic biosensors with nanotechnology continues to drive advancements in glucose monitoring, offering the potential for more accurate, convenient, and user-friendly tools for diabetes management. Current research explores non-invasive glucose monitoring methods, such as using sweat, saliva, or interstitial fluid instead of blood, aiming to reduce the discomfort and inconvenience associated with frequent blood sampling. A review of the advancements and applications of MOF-based enzyme-mimic electrochemical sensors for glucose monitoring can provide valuable insights for young researchers, inspiring future research in biomedical device fabrication. Such reviews not only offer a comprehensive understanding of the current state of the art but also highlight existing challenges and future opportunities in the field of enzyme-less glucose sensing, particularly in the surface modification techniques of highly porous MOFs. This fosters innovation and new research directions. By understanding the advantages, challenges, and opportunities, researchers can contribute to the development of more effective and innovative enzyme-mimic glucose sensing transducers, which are essential for advancing biomedical devices.

Free iron in human serum or non-transferrin-bound iron (NTBI) can generate free radicals and lead to oxidative damage. Moreover, it is highly toxic to various tissues and a vital biomarker related to the iron-loading status of thalassemia and Alzheimer's patients. In NTBI in healthy individuals, NTBI levels are typically less than 1 µM; current NTBI analysis usually requires advanced instrumentation and many-step sample pretreatment. To address this issue, we employed our invented BODIPY derivative, BODIPY-PH, as a fluorescence probe and trapped it onto the microcentrifuge tube lid using tapioca starch. The fluorescence intensity of BODIPY-PH increased with increasing NTBI concentration (turn-on). The developed portable reaction chamber facilitates rapid analysis (∼5 min) using small sample volumes (10 μL sample in a total volume of 600 μL). Under optimum conditions, using the sample-developed portable fluorescence device and fluorescence spectrometer, we achieved impressive limits of detection (LOD) of 0.003 and 0.0015 μM, respectively. Furthermore, the developed sensors show relatively high selectivity toward Fe 3+ over other metal ions and biomolecules (i.e., Fe 2+ , Cr 3+ , Cu 2+ , and glucose). The sensor performance in serum samples of thalassemia patients exhibited no significant difference compared to the labeled value (obtained from standard methods). Overall, the developed fluorescence sensor is suitable for determining NTBI and offers high sensitivity, high selectivity, and a short incubation time (5 min). Moreover, the method requires a limited number of reagents, is simple to use, and uses low-cost equipment to determine NTBI in human serum samples., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)

To advance cervical cancer diagnostics, we propose a state-of-the-art label-free electrochemical immunosensor designed for the simultaneous detection of multiple biomarker proteins (p16 INK4a , p53, and Ki67). This immunosensor is constructed using a polyethyleneimine-coated gold nanoparticles/2D tungsten disulfide/graphene oxide (PEI-AuNPs/2D WS 2 /GO) composite-modified three-screen-printed carbon electrode (3SPCE) array. The 2D WS 2 /GO hybrid provides a large specific surface area for supporting well-dispersed PEI-AuNPs and adsorbed redox-active species, enhancing overall performance. The PEI-AuNPs-decorated 2D WS 2 /GO composite not only improves electrode conductivity but also increases the antibody loading capacity. Redox-active species, including Cd 2+ ions, 2,3-diaminophenazine (DAP), and methylene blue (MB), serve as distinct signaling compounds to quantitatively detect the cervical cancer biomarkers p16 INK4a , p53, and Ki67, respectively. Additionally, the immunosensor demonstrates the detection with high sensitivity, good storage stability, high selectivity, and acceptable reproducibility. This immunosensor demonstrates a good linear relationship with the logarithm of protein concentrations. Additionally, the immunosensor also demonstrates high sensitivity, good storage stability, high selectivity, and acceptable reproducibility. Our promising results and the successful application of the immunosensor in detecting three tumor markers in human serum highlight its potential for clinical diagnosis of cervical cancer., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

A label-free electrochemical immunosensor using a toluidine blue (TB)/porous organic polymer (POP)/two-dimensional molybdenum diselenide (2D MoSe 2 ) nanocomposite is developed for highly sensitive detection of aflatoxin B1 (AFB1) in selected crops. A POP/2D MoSe 2 composite material is employed to modify the surface of a screen-printed carbon electrode (SPCE). Subsequently, TB is adsorbed on the modified SPCE surface, and the resulting TB/POP/2D MoSe 2 composite is then used to construct a biosensor. The new POP/2D MoSe 2 nanocomposite offers a high surface-to-volume area and is a good electroactive and biocompatible adsorbent for loading TB probe and capture antibodies. Adsorbed TB onto the POP/2D MoSe 2 nanocomposite is utilized as a redox probe for the signal amplification unit. This TB/POP/2D MoSe 2 nanocomposite provides good electron transfer properties of TB redox probe, good electrical conductivity, good biocompatibility, and likable adsorption ability, thus obtaining a sufficient immobilization quantity of antibodies for the sensor construction. After immobilization of the anti-AFB1 antibody and blocking with BSA on the composite surface, the immunosensor is obtained for the detection of AFB1. Under optimum conditions, the sensor shows a linear logarithmic range of 2.5-40 ng mL -1 with a limit of detection (LOD) of 0.40 ng mL -1 . The developed sensor provides several advantages in terms of simplicity, low cost, short analysis time, high selectivity, stability, and reproducibility. Additionally, the proposed immunosensor is successfully validated by the detection of AFB1 in rice, corn, and peanut samples. Utilizing the TB/POP/2D MoSe 2 nanocomposite, this label-free electrochemical immunosensor demonstrates outstanding sensitivity and selectivity in detecting AFB1, making it a valuable tool for ensuring the safety of agricultural products and enhancing food security., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

A cutting-edge electrochemical method is presented for precise quantification of amitraz (AMZ), a commonly used acaricide in veterinary medicine and agriculture. Leveraging a lab-made screen-printed carbon electrode modified with a synergistic blend of perylene tetracarboxylic acid (PTCA), mesoporous carbon (MC), and Nafion, the sensor's sensitivity was significantly improved. Fine-tuning of PTCA, MC, and Nafion ratios, alongside optimization of the pH of the supporting electrolyte and accumulation time, resulted in remarkable sensitivity enhancements. The sensor exhibited a linear response within the concentration range 0.01 to 0.70 μg mL -1 , boasting an exceptionally low limit of detection of 0.002 μg mL -1 and a limit of quantification of 0.10 μg mL -1 , surpassing maximum residue levels permitted in honey, tomato, and longan samples. Validation with real samples demonstrated high recoveries ranging from 80.8 to 104.8%, with a relative standard deviation below 10%, affirming the method's robustness and precision. The modified PTCA/MC/Nafion@SPCE-based electrochemical sensor not only offers superior sensitivity but also simplicity and cost-effectiveness, making it a pivotal tool for accurate AMZ detection in food samples. Furthermore, beyond the scope of this study, the sensor presents promising prospects for wider application across various electrochemical analytical fields, thereby significantly contributing to food safety and advancing agricultural practices., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

Thin-film nano-architecting is a promising approach that controls the properties of nanoscale surfaces to increase their interdisciplinary applications in a variety of fields. In this context, zinc oxide (ZnO)-based various nano-architectures (0-3D) such as quantum dots, nanorods/nanotubes, nanothin films, tetrapods, nanoflowers, hollow structures, etc. have been extensively researched by the scientific community in the past decade. Owing to its unique surface charge transport properties, optoelectronic properties and reported biomedical applications, ZnO has been considered as one of the most important futuristic bio-nanomaterials. This review is focused on the design/synthesis and engineering of 0-3D nano-architecture ZnO-based thin films and coatings with tunable characteristics for multifunctional biomedical applications. Although ZnO has been extensively researched, ZnO thin films composed of 0-3D nanoarchitectures with promising thin film device bio-nanotechnology applications have rarely been reviewed. The current review focuses on important details about the technologies used to make ZnO-based thin films, as well as the customization of properties related to bioactivities, characterization, and device fabrication for modern biomedical uses that are relevant. It features biosensing, tissue engineering/wound healing, antibacterial, antiviral, and anticancer activity, as well as biomedical diagnosis and therapy with an emphasis on a better understanding of the mechanisms of action. Eventually, key issues, experimental parameters and factors, open challenges, etc. in thin film device fabrications and applications, and future prospects will be discussed, followed by a summary and conclusion.

This study presents a novel approach for the rapid and reliable electrochemical determination of 5-hydroxymethylfurfural (5-HMF) in honey using a screen-printed carbon electrode modified with nickel oxide nanoparticles (NiONPs/SPCE). The NiONPs were synthesized using a simple method and characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The NiONPs/SPCE demonstrated enhanced sensitivity and selectivity for 5-HMF detection. The electrochemical behavior of 5-HMF on the NiONPs/SPCE was investigated using techniques such as cyclic voltammetry (CV) and square wave voltammetry (SWV). The optimum experimental conditions were obtained including a 5 μL of 5.0 mg/mL NiONPs modifier, the voltammetric response of step potential 15 mV, amplitude 50 mV and frequency 50 Hz in 0.1 M BR buffer pH 13 as supporting electrolyte. The proposed method exhibited a linear relationship between the cathodic peak current and the concentration of 5-HMF in the concentration ranges of 0.5-5.0 ppm, with a limit of detection (LOD) of 0.24 ppm. The selectivity of the NiONPs/SPCE was evaluated by studying potential interferences commonly found in honey samples, and the results demonstrated excellent selectivity for 5-HMF detection. The reproducibility and stability of the NiONPs/SPCE were also assessed, with low relative standard deviations (RSD) obtained for both the cathodic peak current (2.94 %) and long-term stability (3.14 %). The developed NiONPs/SPCE method was successfully applied to the determination of 5-HMF in real honey samples, yielding comparable results to the standard HPLC method. This work showcases the potential of the NiONPs/SPCE as a practical and cost-effective electrochemical sensor for the accurate analysis of 5-HMF in honey samples., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Fucosylated alpha-fetoprotein (AFP-L3) is a more specific and sensitive biomarker for early diagnosis of hepatocellular carcinoma (HCC) than only the alpha-fetoprotein (AFP) level. Rapid and simple detection of AFP-L3 level greatly facilitates the early detection as well as the treatment of HCC, resulting in the reduction of mortality. Here, we developed a rapid and sensitive lateral flow assay (LFA) using lectin Lens culinaris agglutinin (LCA), which has a specific affinity to AFP-L3 fraction of AFP, as a biorecognition element for determination of the fucosylation of AFP. The assay is based on a sandwich format performed on a lateral flow test strip. LCA was immobilized on the membrane as a test line (T). Quantitative detection of AFP-L3 was achieved by measuring the green color intensity of captured gold nanoparticle conjugates on the T and control line (C) utilizing an in-house test strip reader. The calculated absorbance obtained by the green color intensity signals proportionally increased with AFP concentrations. The developed lectin-based LFA provided a detection limit of 0.8 ng/mL for AFP with a linear range between 1.5 and 160.0 ng/mL within an assay time of 10 min. Recoveries between 74.5% and 113.2% with relative standard deviations of 5.2%-8.7% for measuring spiked human serum were also achieved. The results reveal that the proposed assay offers a rapid, sensitive, and specific method, which is useful for development in point-of-care testing for early detection and treatment of HCC., (© 2023 Wiley Periodicals LLC.)

We developed a novel, sensitive, and selective platform for the specific determination of aflatoxin B1 (AFB1). Single-walled carbon nanohorns decorated by a cobalt oxide composite and gold nanoparticles were created to provide facile electron transfer and improve the sensor's sensitivity. In addition, we attributed the selectivity of the proposed sensor to the specific binding property of the anti-aflatoxin B1 antibody. We clarified the specific interaction of the proposed immunosensor to AFB1 using homology modeling combined with molecular docking. In the presence of AFB1, the current signal of the modified electrode reduced; this involved specific antibody-antigen binding, including hydrophobic hydrogen bonding and pi-pi stack interactions. The new AFB1 sensor platform showed two linearity ranges of 0.01-1 ng mL -1 and 1-100 ng mL -1 , with the limit of detection at 0.0019 ng mL -1 . We investigated the proposed immunosensor in real samples, including peanuts, certified reference material of a peanut sample (labeled 206 μg kg -1 AFB1), corn, and chicken feed. The sensor's accuracy was 86.1-104.4% recovery, which agrees with the reference HPLC technique using paired t-test analysis. The present work shows excellent performance for AFB1 detection and could be applied for food quality control or modified to detect other mycotoxins., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

The quantification of alpha-fetoprotein (AFP) as a potential liver cancer biomarker which is generally found in ultratrace level is of significance in biomedical diagnostics. Therefore, it is challenging to find a strategy to fabricate a highly sensitive electrochemical device towards AFP detection through electrode modification for signal generation and amplification. This work shows the construction of a simple, reliable, highly sensitive, and label-free aptasensor based on polyethyleneimine-coated gold nanoparticles (PEI-AuNPs). A disposable ItalSens screen-printed electrode (SPE) is employed for fabricating the sensor by successive modifying with PEI-AuNPs, aptamer, bovine serum albumin (BSA), and toluidine blue (TB), respectively. The AFP assay is easily performed when the electrode is inserted into a small Sensit/Smart potentiostat connected to a smartphone. The readout signal of the aptasensor derives from the electrochemical response of TB intercalating into the aptamer-modified electrode after binding with the target. The decrease in current response of the proposed sensor is proportional to the AFP concentration due to the restriction of the electron transfer pathway of TB by a number of insulating AFP/aptamer complexes on the electrode surface. PEI-AuNPs improve SPE's reactivity and provide a large surface area for aptamer immobilization whereas aptamer provides selectivity to the target AFP. Consequently, this electrochemical biosensor is highly sensitive and selective for AFP analysis. The developed assay reveals a linear range of detection from 10 to 50000 pg mL -1 with R 2 = 0.9977 and provided a limit of detection (LOD) of 9.5 pg mL -1 in human serum. With its simplicity and robustness, it is anticipated that this electrochemical-based aptasensor will be a benefit for the clinical diagnosis of liver cancer and further developed for other biomarkers analysis., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Yaiwong, Anuthum, Sangthong, Jakmunee, Bamrungsap and Ounnunkad.)

The study aimed to investigate the effects of two different plasma systems, including pinhole plasma jet and gliding arc (GA) plasma, for the degradation of herbicide, diuron, in plasma activated solutions (PAS). In the GA plasma system, air was used to generate plasma, however, Ar, oxygen and nitrogen at different gas compositions were compared in the pinhole plasma jet system. The Taguchi design model was used to study the effects of gas compositions. Results revealed that the pinhole plasma jet system was able to degrade over 50% of the diuron in 60 minutes. The optimal plasma generation condition for the highest degradation of diuron used pure Ar gas. The highest degradation percentage of herbicide in PAS corresponded to the lowest hydrogen peroxide (H 2 O 2 ) content, nitrite concentration and electrical conductivity (EC) of the PAS. The diuron degradation products were identified as 3,4-dichloro-benzenamine, 1-chloro-3-isocyanato-benzene and 1-chloro-4-isocyanato-benzene via gas chromatography-mass spectrometry (GC-MS). The GA plasma system was not adequate for the degradation of herbicide in PAS., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Human immunodeficiency virus (HIV) causing acquired immune deficiency syndrome (AIDS) is still a global issue. Long-term drug treatment and nonadherence to medication increase the spread of drug-resistant HIV strains. Therefore, the identification of new lead compounds is being investigated and is highly desirable. Nevertheless, a process generally necessitates a significant budget and human resources. In this study, a simple biosensor platform for semi-quantification and verification of the potency of HIV protease inhibitors (PIs) based on electrochemically detecting the cleavage activity of the HIV-1 subtype C-PR (C-SA HIV-1 PR) was proposed. An electrochemical biosensor was fabricated by immobilizing His6-matrix-capsid (H 6 MA-CA) on the electrode surface via the chelation to Ni 2+ -nitrilotriacetic acid (NTA) functionalized GO. The functional groups and the characteristics of modified screen-printed carbon electrodes (SPCE) were characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS). C-SA HIV-1 PR activity and the effect of PIs were validated by recording changes in electrical current signals of the ferri/ferrocyanide redox probe. The detection of PIs, i.e., lopinavir (LPV) and indinavir (IDV), toward the HIV protease was confirmed by the decrease in the current signals in a dose-dependent manner. In addition, our developed biosensor demonstrates the ability to distinguish the potency of two PIs to inhibit C-SA HIV-1 PR activities. We anticipated that this low-cost electrochemical biosensor would increase the efficiency of the lead compound screening process and accelerate the discovery and development of new HIV drugs., Competing Interests: The authors declare no competing financial interest., (© 2023 The Authors. Published by American Chemical Society.)

The miniaturization of analytical systems and the utilization of nontoxic natural extract from plants play significant roles for green analytical chemistry methodology. In this work, the microfluidic hydrodynamic sequential injection (HSI) with the LED-phototransistor colorimetric detection system has been proposed to create an ecofriendly and low-cost miniaturized analytical system for online determination of iron in water samples using Curcuma putii Maknoi & Jenjitt. extracts as high stability and good selectivity of a natural reagent. The proposed method was designed for online solution mixing and colorimetric detection on a microfluidic platform. The Curcuma putii Maknoi & Jenjitt. extracts and standard/samples were sequentially aspirated to fill the channel before entering the built-in flow cell. The intensity of iron- Curcuma putii Maknoi & Jenjitt. extract complex was monitored under the optimum conditions of flow rate, sample volume, mixing zone length, and aspiration sequences, by altering the gain control of the colorimetric detector to achieve good sensitivity. The results demonstrated a good performance of the green analytical systems. A linear calibration graph in the range of 0.5-6.0 mg L -1 was obtained with a limit of detection at an adequate level of 0.11 mg L -1 for water samples with a sample throughput of 30 h -1 . The precise and accurate measurement results were achieved with relative standard deviations in the range of 1.61-1.72%, and percent recoveries were found in the range of 90.6-113.4. The proposed method offers cost-effective, easy operation over an appropriate analysis time (2 min/injection) with good sensitivity and is environmentally friendly with low consumption of solutions and the use of high stability and good selectivity of nontoxic reagents. The achieved method was demonstrated to be a good choice for routine analysis., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2023 Maneerat Namjan et al.)

Fabrication of a new three-dimensional Cu(II) metal-organic framework, {[Cu 4 (4,4'-bipy) 3 (OH) 2 (mal) 3 ]·4H 2 O} n ( 1a ; 4,4'-bipy = 4,4'-bipyridine, H 2 mal = malonic acid; P 2 1 / m ), that undergoes an unprecedented redox-versatile ligand-substitution-induced single-crystal to single-crystal transformation, for smartphone-based detection of iodide was studied. The Cu-MOF 1a has been effortlessly synthesized by the microwave-heating technique. Phase formation of the Cu-MOF 1a depended on counter-anions. The transformations can be triggered by halides to corresponding coordination polymers through both non-redox and redox-associated pathways. The changes in the local structure and oxidation state of copper during the transformation were studied by ex situ and in situ synchrotron X-ray absorption spectroscopies. The selectivity of the halide-triggered transformation was investigated. A study on smartphone-based colorimetric detection of iodide was found to be linearly proportional to the iodide concentration in the range 10-1500 mg/L with a limit of detection of 5 mg/L and good precision relative standard deviation of 1.9% ( n = 11), possibly to construct the iodide test kit.

Liver cancer is one of the most common global health problems that features a high mortality rate. Alpha-fetoprotein (AFP) is a potential liver cancer biomarker for the diagnosis of liver cancer. The quantitative detection of AFP at an ultratrace level has important medical significance. Using the reaction of the antibody-antigen pair in an immunosensor enables the sensitive and selective AFP assay. Finding a strategy in signal generation and amplification is challenging to fabricate new sensitive electrochemical immunosensors for AFP detection. This study demonstrates the construction of a simple, reliable, and label-free immunosensor for the detection of AFP on a smart phone. Exfoliated two-dimensional (2D) molybdenum diselenide (MoSe 2 ) and 2D tungsten diselenide (WSe 2 ) were employed to modify the disposable screen-printed carbon electrode (SPCE) to use as the electrochemical platform, which is affixed to a small potentiostat connected to a smart phone. The modified electrode offers antibody immobilization and allows detection of AFP via an immunocomplex forming a sandwich-like configuration with the AFP-corresponding aptamer. A heterojunction 2D MoSe 2 /2D WSe 2 composite improves the SPCE's reactivity and provides a large surface area and good adsorption capacity for the immobilizing antibodies. The signal generation for the immunosensor is from the electrochemical response of methylene blue (MB) intercalating into the aptamer bound on the electrode. The response for the proposed sandwich-like immunosensor is proportional to the AFP concentration (1.0-50,000 pg ml -1 ). The biosensor has potential for the development of a simple and robust point-of-care diagnostic platform for the clinical diagnosis of liver cancer, achieving a low limit of detection (0.85 pg ml -1 ), high sensitivity, high selectivity, good stability, and excellent reproducibility., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Chanarsa, Jakmunee and Ounnunkad.)

This work aims to develop a more robust, easy-to-use, low-pressure, and cost-effective nonsuppressed open tubular capillary ion chromatography (NS-OTIC) approach with online dialysis for small ions determination in drinks. The fabricated device was applied for two selected columns, including poly(butadiene-maleic acid) on a 50-μm bore and AS18 Latex on 25-µm bore fused silica capillaries, for the separation of mixed cations (Na + , K + , Ca 2+ , Mg 2+ ) and mixed anions (Cl - , Br - , NO 2 - , NO 3 - ), respectively. High concentrations of ions (up to 100 mM) level can be directly introduced into the NS-OTIC system without an off-line (manual) dilution step. The linear relationship of the peak area and concentration of model ions can be obtained with a resolution > 1.1. The repeatability of the peak area for both OTIC columns was < 6% RSD. Juice and tea samples were successfully analyzed with % recoveries of 77-112 and 90-119 for cation and anion determinations, respectively., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Pd-based catalysts consisting of Pd nanoparticles on nitrogen-doped carbon quantum dots (N-CQDs) modified silica (SiO 2 ) and reduced graphene oxide have been synthesized through reduction for use as catalysts for improved formic acid oxidation. The structure, morphology, chemical composition, functional groups, and porosity of the synthesized catalysts were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR) spectroscopy, Raman spectroscopy, and Brunauer-Emmett-Teller (BET) spectroscopy, respectively. Their electrocatalytic activities were also evaluated by electrochemical measurements. The differences in the average particle sizes found for Pd/N-CQDs-SiO 2 -rGO, Pd/N-CQDs-rGO, and Pd/rGO were 4.81, 5.56, and 6.31 nm, respectively. It was also found that the Pd/ x N-CQDs-SiO 2 - y rGO composite catalysts (where x and y is 1 to 4) can significantly improve the activity and stability toward formic acid electrooxidation compared with Pd/rGO and commercial Pt/C. The mass activities of Pd/N-CQDs-SiO 2 -rGO, Pd/N-CQDs-rGO, and Pd/rGO were 951.4, 607.8, and 157.6 mA g -1 , respectively, which was ca. 6-7 times compared with Pd/rGO and approximately 3-4 times compared with commercial Pt/C. With low potential for CO oxidation and high current intensity, the composites of rGO, SiO 2 , and N-CQDs into Pd-based catalysts improved the catalytic activity of the prepared catalyst for the oxidation of formic acid in acidic media. The value of the Tafel slope designated that the chief path of the prepared catalysts is the dehydrogenation process. These prepared catalysts exhibit promise toward the development of high-performance Pd-based electrocatalysts for formic acid oxidation., Competing Interests: The authors declare no competing financial interest., (© 2022 The Authors. Published by American Chemical Society.)

A portable paper-based electrochemical sensor has been developed to determine 5-hydroxymethylfurfural (5-HMF). A screen-printed carbon electrode (SPCE) was facilely fabricated for the first time on poster paper which showed a very satisfactory electrochemical response. The analytical performance of the electrode was enhanced by electrochemical deposition of silver microdendrites (AgMDs). The cathodic peak of 5-HMF occurred at approximately -1.48 V, lower than that obtained from the bare poster-SPCE. Moreover, the modified electrode showed a higher current response than the bare electrode, revealing that the AgMDs not only exhibited highlighted electrocatalytic features but also improved the electrical conductivity and increased the electrode surface area. Afterward, some influencing conditions were optimized, including scan rate and the number of scan cycles for AgMD deposition, pH, temperature, and square wave voltammetric parameters. Under the optimal conditions, the analytical characteristics of the proposed sensor were evaluated. The cathodic peak current increased linearly according to 5-HMF concentration over the range of 3-100 ppm, and the detection limit was 1.0 ppm. This low-cost, disposable electrochemical sensor provided environmentally friendly, simple and rapid detection, acceptable precision, good stability, and high selectivity. Additionally, this method can be applied to quantify 5-HMF in honey samples with satisfying accuracy.

A dual-mode electrochemical biosensor is successfully developed for simultaneous detection of two different kinds of breast cancer biomarkers, namely cancer antigen 15-3 (CA 15-3) and microRNA-21 (miRNA-21), for the first time. The sensor composes of a poly(3-aminobenzylamine)/two-dimensional (2D) molybdenum selenide/graphene oxide nanocomposite modified two-screen-printed carbon electrode array (dual electrode), functionalized individually with 2,3-diaminophenazine-gold nanoparticles and toluidine blue-gold nanoparticles. Both kinds of the redox probe-gold nanoparticles are employed as signaling molecules and supports for immobilization of anti-CA 15-3 antibodies and capture DNA-21 probes, respectively. Due to the good conductivity and high surface-to-volume ratio of the nanocomposite, high amount of the antibodies and capture probes can be immobilized on the modified dual-electrode, giving the efficient duplex detection. Consequently, the biosensor provides good selectivity, and high sensitivity for the dual target analyte detection. The experimental results show that this label-free biosensor exhibits good linear responses to the concentrations of both target analytes with the limits of detection (LODs) of 0.14 U mL -1 and 1.2 fM for CA 15-3 and miRNA-21, respectively. This assay strategy has a great potential to be further developed for the simultaneous detection of a variety of miRNAs and protein biomarkers for point-of-care (POC) diagnostic applications., (Copyright © 2021 Elsevier B.V. All rights reserved.)

A cost-effective long-path absorption liquid core waveguide (LCW) device was fabricated from a typical Teflon tube, and using a high refractive index (RI) medium of acid. It was incorporated in a flow injection-gas diffusion system for the sensitive determination of sulfide via methylene blue (MB) chemistry. The gas diffusion unit could prevent some interferences in samples. The limit of detection of 0.13 μmol L -1 was achieved without using a preconcentration procedure. The proposed device was successfully applied for sulfide determination in canal water samples with 88.7 - 103% recovery.

Methylene blue (MB) adsorption onto a two-dimensional molybdenum disulfide (2D MoS 2 )/graphene oxide (GO) nanocomposite sitting on a screen-printed carbon electrode (SPCE) is used to develop a new sensitive label-free electrochemical immunosensor for the detection of matrix metalloproteinase-7 (MMP-7) cancer biomarkers. The 2D MoS 2 /GO nanocomposite deposited onto an SPCE provides a large specific surface area, fast electron transfer, and exceptional electrical conductivity. Furthermore, MB adsorbed onto the 2D MoS 2 /GO nanocomposite architecture can be used for signal amplification in electrochemical immunosensors. Moreover, an immunosensor platform was fabricated by the adsorption of anti-MMP-7 capture antibodies onto the MB/2D MoS 2 /GO nanocomposite surface via electrostatic interactions for the detection of the MMP-7 immunocomplex. Under optimum conditions, the label-free immunosensor exhibits a decrease in the current response for MB corresponding to the MMP-7 concentration. The sensor affords a linear logarithmic range of 0.010-75 ng mL -1 with a limit of detection (LOD) of 0.007 ng mL -1 . The developed electrochemical immunosensor provides high selectivity, good reproducibility, and excellent stability. Furthermore, the proposed immunosensor can be applied for the detection of MMP-7 in human serum samples with good recovery. Thus, this device can be applied for the early clinical diagnosis of pancreatic and colorectal cancers., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021. Published by Elsevier B.V.)

A new electrochemical immunosensor is developed for the label-free simultaneous detection of mucin1 (MUC1), cancer antigen 15-3 (CA15-3), and human epidermal growth factor receptor 2 (HER2) early breast cancer biomarkers. The biosensor is simply designed using the deposition of three different systems of redox species-antibody-conjugated polyethylenimine coated-gold nanoparticles (PEI-AuNPs), for the first time. The screen-printed carbon electrode (SPCE) comprising a three-working electrode array is modified with the conjugated PEI-AuNPs. Multiplex sensing is performed by utilizing the distinguishable electrochemical responses of the redox-active species; anthraquinone-2-carboxylic acid (AQ), thionine chloride (TH), and AgNO 3 (Ag + ) on the PEI-AuNPs conjugates for the detection of MUC1, CA15-3, and HER2, respectively. The single-run determination of the biomarkers by the proposed immunosensor is carried out by measuring the decrease (%) in the oxidation peak currents due to the formation of three kinds of antibody-antigen complexes. The decreased currents are logarithmically proportional to the antigen concentrations in the ranges of 0.10-100 U mL -1 CA15-3 and 0.10-100 ng mL -1 MUC1 and HER2 with detection limits of 0.21 U mL -1 , 0.53 ng mL -1 and 0.50 ng mL -1 , respectively, which are significantly lower than the clinically relevant cut-off levels. The sensor reveals high selectivity and satisfactory reproducibility. After storing for two weeks, the sensor retains the responses with ca. 90% of the initial currents. The immunosensor is successfully applied to detect three tumor markers in human serum and can provide a new technological platform for the development of low-cost, highly stable, sensitive, selective, and point-of-care (POC) diagnosis.

A simple, rapid, and cost-effective flow injection amperometric (FI-Amp) sensor for sensitive determination of uric acid (UA) was developed based on a new combination of carbon black (CB) and graphene oxide (GO) modified screen-printed carbon electrode (SPCE). The CB-GO nanocomposites were simply synthesized and modified on the working electrode surface to increase electrode conductivity and enhance the sensitivity of UA determination via the electrocatalytic activity toward UA oxidation. The morphologies and electrochemical properties of the synthesized nanomaterials were investigated through scanning electron microscopy (SEM), transmission electron microscopy (TEM), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV). The modified electrode was incorporated with FI-Amp to improve UA detection's sensitivity, stability, and automation. Some parameters affecting sensitivity were optimized, including pH of the electrolyte solution, applied potential, amount of CB-GO suspension, flow rate, injection volume, and reaction coil length. Using an applied potential of +0.35 V (vs Ag/AgCl), the anodic current was linearly proportional to UA concentration over the range of 0.05-2000 μM with a detection limit of 0.01 μM (3 S/N). Besides, the developed method provides a sample throughput of 25 injections h -1 , excellent sensitivity (0.0191 μA/μM), selectivity, repeatability (RSD 3.1%, n = 7), and stability (RSD 1.08%, n = 50). The proposed system can tolerate potential interferences commonly found in human urine. Furthermore, a good correlation coefficient between the results obtained from the FI-Amp sensor and a hospital laboratory implies that the proposed system is accurate and can be utilized for UA detection in urine samples., (Copyright © 2021 Elsevier B.V. All rights reserved.)