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1. Anthropogenic releases of radiation are of ongoing importance for environmental protection, but the radiation doses at which natural systems begin to show effects are controversial. More certainty is required in this area to achieve optimal regulation for radioactive substances. We recently carried out a large survey (268 sampled animals and 20 sites) of the association between environmental radiation exposures and small mammal gut-associated microbiomes (fungal and bacterial) in the Chornobyl Exclusion zone (CEZ). Using individual measurements of total absorbed dose rates and a study design and analyses that accounted for spatial non-independence, we found no, or only limited, association. 2. Watts et al. have criticised our study: for not filtering candidate non-resident components prior to our fungal microbiome analyses, for our qualified speculations on the relative merits of faecal and gut samples, and for the design of our study which they felt lacked sufficient replication. 3. The advantage of filtering non-resident-fungal taxa is not clear and it would not have changed the null (spatially adjusted) association we found between radioactive dose and mycobiome composition because the most discriminatory fungal taxa with regard to dose were non-resident taxa. 4. We maintain that it was legitimate for us to make qualified discussion comments on the differences in results between our faecal and gut microbiome analyses and on the relative merits of these sample types. 5. Most importantly, the criticism of our study design by Watts et al. and the designs and analysis of their recent studies in the CEZ show a misunderstanding of the true nature of independent replication in field studies. Recognising the importance of spatial non-independence is essential in the design and analysis of radioecological field surveys.

Seasonal patterns in immunity are frequently observed in vertebrates but are poorly understood. Here we focussed on a natural piscine model, the three-spined stickleback (Gasterosteus aculeatus), and asked how seasonal immune allocation is driven by physical variables (time, light and heat). Using functionally-relevant gene expression metrics as a reporter of seasonal immune allocation we synchronously sampled fish monthly from the wild (two habitats), and from semi-natural outdoors mesocosms (stocked from one of the wild habitats). This was repeated across two annual cycles, with continuous within-habitat monitoring of environmental temperature and implementing a manipulation of temperature in the mesocosms. We also conducted a long-term laboratory experiment, subjecting acclimated wild fish to natural and accelerated (× 2) photoperiodic change at 7 and 15°C. The laboratory experiment demonstrated that immune allocation was independent of photoperiod and only a very modest effect, at most, was controlled by a tentative endogenous circannual rhythm. On the other hand, experimentally-determined thermal effects were able to quantitatively predict much of the summer-winter fluctuation observed in the field and mesocosms. Importantly, however, temperature was insufficient to fully predict, and occasionally was a poor predictor of, natural patterns. Thermal effects can thus be over-ridden by other (unidentified) natural environmental variation and do not take the form of an unavoidable constraint due to cold-blooded physiology. This is consistent with a context-dependent strategic control of immunity in response to temperature variation, and points to the existence of temperature-sensitive regulatory circuits that might be conserved in other vertebrates.

Transcriptomic methods are set to revolutionize the study of\ud the immune system in naturally occurring nonmodel organisms.\ud With this in mind, the present article focuses on ways in which the use of ‘nonmodel’ rodents (not the familiar laboratory species) can advance studies into the classical, but ever relevant, epidemiologic triad of immune defence, infectious disease and environment. For example, naturally occurring rodents are an interesting system in which to study the environmental stimuli that drive the development and homeostasis of the immune system and, by extension, to\ud identify where these stimuli are altered in anthropogenic\ud environments leading to the formation of immunopathological\ud phenotypes. Measurement of immune expression may help define individual heterogeneity in infectious disease susceptibility and transmission and facilitate our understanding of infection dynamics and risk in the natural environment; furthermore, it may provide a means of surveillance that can filter individuals carrying previously unknown acute infections of potential ecological or zoonotic importance. Finally, the study of immunology in wild animals may reveal interactions within the immune system and between immunity and other organismal traits that are not observable under restricted laboratory conditions. Potentiating much of this is the possibility of combining gene expression profiles with analytical tools derived from ecology and systems biology to reverse engineer interaction networks between immune responses, other organismal traits and the environment (including symbiont exposures), revealing regulatory architecture.\ud Such holistic studies promise to link ecology, epidemiology\ud and immunology in natural systems in a unified approach that can illuminate important problems relevant to\ud human health and animal welfare and production.

We report a PCR survey of hantavirus infection in the extensive field vole (Microtus agrestis) populations occurring in the Kielder Forest, northern England. A Tatenale virus-like lineage was frequently detected (~ 15% prevalence) in liver tissue. Such lineages are likely to be endemic in northern England.

A large-scale field study in naturally occurring vole populations identified gene expression changes over time and demonstrates how wild mammals exhibit tolerance to chronic parasite infections., Hosts are likely to respond to parasitic infections by a combination of resistance (expulsion of pathogens) and tolerance (active mitigation of pathology). Of these strategies, the basis of tolerance in animal hosts is relatively poorly understood, with especially little known about how tolerance is manifested in natural populations. We monitored a natural population of field voles using longitudinal and cross-sectional sampling modes and taking measurements on body condition, infection, immune gene expression, and survival. Using analyses stratified by life history stage, we demonstrate a pattern of tolerance to macroparasites in mature compared to immature males. In comparison to immature males, mature males resisted infection less and instead increased investment in body condition in response to accumulating burdens, but at the expense of reduced reproductive effort. We identified expression of the transcription factor Gata3 (a mediator of Th2 immunity) as an immunological biomarker of this tolerance response. Time series data for individual animals suggested that macroparasite infections gave rise to increased expression of Gata3, which gave rise to improved body condition and enhanced survival as hosts aged. These findings provide a clear and unexpected insight into tolerance responses (and their life history sequelae) in a natural vertebrate population. The demonstration that such responses (potentially promoting parasite transmission) can move from resistance to tolerance through the course of an individual's lifetime emphasises the need to incorporate them into our understanding of the dynamics and risk of infection in the natural environment. Moreover, the identification of Gata3 as a marker of tolerance to macroparasites raises important new questions regarding the role of Th2 immunity and the mechanistic nature of the tolerance response itself. A more manipulative, experimental approach is likely to be valuable in elaborating this further., Author Summary Hosts do not always resist parasites. And once infection establishes, relatively little is known of how naturally occurring hosts tolerate (mitigate for) adverse effects, or what the life history consequences of this tolerance may be. In this article we demonstrate a pattern of tolerance to parasitic worms and arthropods in wild voles and link this to increased expression of an immunological biomarker. The biomarker, Gata3, is triggered by infection, precedes significant changes in body condition, and impacts on fecundity and survival. These results point to the considerable ecological importance of tolerance in wild vertebrates and to how poorly it is understood, while at the same time giving a new perspective on the natural function of immune response pathways involving Gata3.

Research on the interactions between the field voles (Microtus agrestis) of Kielder Forest and their natural parasites dates back to the 1930s. These early studies were primarily concerned with understanding how parasites shape the characteristic cyclic population dynamics of their hosts. However, since the early 2000s, research on the Kielder field voles has expanded considerably and the system has now been utilized for the study of host–parasite biology across many levels, including genetics, evolutionary ecology, immunology and epidemiology. The Kielder field voles therefore represent one of the most intensely and broadly studied natural host–parasite systems, bridging theoretical and empirical approaches to better understand the biology of infectious disease in the real world. This article synthesizes the body of work published on this system and summarizes some important insights and general messages provided by the integrated and multidisciplinary study of host–parasite interactions in the natural environment. Fil: Turner, A. K.. University of Liverpool; Reino Unido Fil: Beldomenico, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. University of Liverpool; Reino Unido Fil: Bown, K.. University of Liverpool; Reino Unido. University of Salford; Reino Unido Fil: Burthe, S. J.. University of Liverpool; Reino Unido. Natural Environmental Research Council. Centre for Ecology & Hydrology; Reino Unido Fil: Jackson, J. A.. University of Liverpool; Reino Unido. University of Aberystwyth. Institute of Biological, Environmental and Rural Sciences; Reino Unido Fil: Lambin, X.. University Of Aberdeen; Reino Unido Fil: Begon, M.. University of Liverpool; Reino Unido

SUMMARYEven the most generalist parasites usually occur in only a subset of potential host species, a tendency which reflects overriding environmental constraints on their distributions in nature. The periodic shifting of these limitations represented by host-switches may have been an important process in the evolution of many host-parasite assemblages. To study such events, however, it must first be established where and when they have occurred. Past host-switches within a group of parasites are usually inferred from a comparison of the parasite phylogeny with that of the hosts. Congruence between the phylogenies is often attributed to a history of association by descent with cospeciation, and incongruence to host-switching or extinction in ‘duplicated’ parasite lineages (which diverged without a corresponding branching of the host tree). The inference of host-switching from incongrucnt patterns is discussed. Difficulties arise because incongruence can frequently be explained by different combinations of biologically distinct events whose relative probabilities are uncertain. Also, the models of host parasite relationships implicit in historical reconstructions may often not allow for plausible sources of incongruence other than host-switching or duplication/extinction, or for the possibility that colonization could, in some circumstances, be disguised by ‘false’ congruence.

We use teleseismic waveform inversion, along with depth phase analysis, to constrain the centroid depths and source parameters of large African earthquakes. The majority of seismic activity is concentrated along the East African Rift System, with additional active regions along stretches of the continental margins in north and east Africa, and in the Congo Basin. We examine variations in the seismogenic thickness across Africa, based on a total of 227 well-determined earthquake depths, 112 of which are new to this study. Seismogenic thickness varies in correspondence with lithospheric thickness, as determined from surface wave tomography, with regions of thick lithosphere being associated with seismogenic thicknesses of up to 40 km. In regions of thin lithosphere, the seismogenic thickness is typically limited to ≤20 km. Larger seismogenic thicknesses also correlate with regions that have dominant tectonothermal ages of ≥1500 Ma, where the East African Rift passes around the Archean cratons of Africa, through the older Proterozoic mobile belts. These correlations are likely to be related to the production, affected by method and age of basement formation, and preservation, affected by lithospheric thickness, of a strong, anhydrous lower crust. The Congo Basin contains the only compressional earthquakes in the continental interior. Simple modelling of the forces induced by convective support of the African plate, based on long-wavelength free-air gravity anomalies, indicates that epeirogenic effects are sufficient to account for the localization and occurrence of both extensional and compressional deformation in Africa. Seismicity along the margins of Africa reflects a mixture between oceanic and continental seismogenic characteristics, with earthquakes in places extending to 40 km depth.

We have reported the case of a 30-year-old woman with Cushing's disease who died of massive pulmonary thromboembolism 5 weeks after successful transsphenoidal hypophysectomy. Glucocorticoid excess appears to cause a hypercoagulable state, and consideration of this thromboembolic propensity and its potential duration after cure is indicated in all patients with Cushing's syndrome during the perioperative period. At the present time, we recommend the routine perioperative use of intermittent pneumatic compression in all patients with Cushing's disease or Cushing's syndrome.

55 male Ss were assigned to 5 groups: control, hypnosis alone, motivation alone, low susceptible hypnosis with motivation, or high susceptible hypnosis with motivation. Ss performed 2 runs on a treadmill to their maximum capacity, as measured by oxygen consumption, blood lactate concentration, and respiratory quotient. Groups involving hypnosis performed in the posthypnotic state. A significant increase in endurance performance was revealed in the motivation alone Ss and in high susceptible hypnosis Ss who were given motivational suggestions. Maximum ventilation was significantly increased in high susceptible hypnosis Ss when compared with control Ss and significant increases in blood lactate concentrations were revealed when the high susceptible hypnosis Ss were compared with low susceptible hypnosis and motivation alone Ss. The reasons for the changes in metabolic variables are discussed. Findings demonstrated that in achieving greater endurance performance, motivational suggestions alone are a...

A 66-year-old man presented with height loss, back pain due to an L4 vertebral compression deformity, and marked osteopenia shown by radial photon absorptiometry. The patient had small testes and a long history of infertility and impotence. Plasma testosterone was low (130 ng/dL; normal, 300 to 1000 ng/dL) with marked elevation of serum prolactin (590 ng/mL; normal, 0 to 15 ng/mL). High-resolution computed tomography showed an invasive pituitary macroadenoma with a secondary empty sella turcica. Bone histomorphometry showed low-normal trabecular bone volume with normal formation and resorption indices. The patient was treated with calcium carbonate supplements, testosterone replacement, and bromocriptine mesylate without subsequent progression of the osteoporosis. This first report of hyperprolactinemic hypogonadism in a man who presented with overt osteoporosis shows the importance of evaluating gonadal function in all osteoporotic men.

Great whales have been detected using very-high-resolution satellite imagery, suggesting this technology could be used to monitor whales in remote areas. However, the application of this method to whale studies is at an early developmental stage and several technical factors need to be addressed, including capacity for species differentiation and the maximum depth of detection in the water column. Both require knowledge of the spectral reflectance of the various whale species just above the sea surface, as when whales bodies break the surface of the water to breath, log or breach, there is, theoretically, no sea water between the whale’s skin and the satellite sensor. Here we tested whether such reflectance could be measured on dead whale tissue. We measured the spectral reflectance of fresh integument collected during the bowhead subsistence harvest, and of thawed integument samples from various species obtained following strandings and stored at -20°C. We show that fresh and thawed samples of whale integument have different spectral properties. The reflectance of fresh samples was higher than the reflectance of thawed samples, as integument appears to darken after death and with time, even under frozen conditions. In this study, we present the first whale reflectance estimates (without the influence of sea water and for dead tissue). These provide a baseline for additional work, needed to advance the use of satellite imagery to monitor whales and facilitate their conservation.

© 2018 The Authors. Marine Mammal Science published by Wiley Periodicals, Inc. on behalf of Society for Marine Mammalogy. Large-bodied animals such as baleen whales can now be detected with very high resolution (VHR) satellite imagery, allowing for scientific studies of whales in remote and inaccessible areas where traditional survey methods are limited or impractical. Here we present the first study of baleen whales using the WorldView-3 satellite, which has a maximum spatial resolution of 31 cm in the panchromatic band, the highest currently available to nonmilitary professionals. We manually detected, described, and counted four different mysticete species: fin whales (Balaenoptera physalus) in the Ligurian Sea, humpback whales (Megaptera novaeangliae) off Hawaii, southern right whales (Eubalaena australis) off Península Valdés, and gray whales (Eschrichtius robustus) in Laguna San Ignacio. Visual and spectral analyses were conducted for each species, their surrounding waters, and nonwhale objects (e.g., boats). We found that behavioral and morphological differences made some species more distinguishable than others. Fin and gray whales were the easiest to discern due to their contrasting body coloration with surrounding water, and their prone body position, which is proximal to the sea surface (i.e., body parallel to the sea surface). These results demonstrate the feasibility of using VHR satellite technology for monitoring the great whales.

A bipolar disjunction is an extreme, yet common, biogeographic pattern in non-vascular plants, yet its underlying mechanisms (vicariance or long-distance dispersal), origin and timing remain poorly understood. Here, combining a large-scale population dataset and multiple dating analyses, we examine the biogeography of four bipolar Polytrichales mosses, common to the Holarctic (temperate and polar Northern Hemisphere regions) and the Antarctic region (Antarctic, sub-Antarctic, southern South America) and other Southern Hemisphere (SH) regions. Our data reveal contrasting patterns, for three species were of Holarctic origin, with subsequent dispersal to the SH, while one, currently a particularly common species in the Holarctic (Polytrichum juniperinum), diversified in the Antarctic region and from here colonized both the Holarctic and other SH regions. Our findings suggest long-distance dispersal as the driver of bipolar disjunctions. We find such inter-hemispheric dispersals are rare, occurring on multi-million-year timescales. High-altitude tropical populations did not act as trans-equatorial 'stepping-stones', but rather were derived from later dispersal events. All arrivals to the Antarctic region occurred well before the Last Glacial Maximum and previous glaciations, suggesting that, despite the harsh climate during these past glacial maxima, plants have had a much longer presence in this southern region than previously thought.

© 2018 Biersma, Jackson, Stech, Griffiths, Linse and Convey. From glacial reconstructions it is clear that Antarctic terrestrial life must have been extremely limited throughout Quaternary glacial periods. In contrast, recent biological studies provide clear evidence for long-term in situ persistence throughout glacial times within most extant Antarctic faunal and several microbial groups. However, even now, the evolutionary history of the Antarctic flora-despite playing major role in Antarctic ecosystems-remains poorly studied. We assessed the diversity, richness and relative age divergences within Schistidium (Grimmiaceae, Bryophyta), the most species-rich plant genus in the Antarctic, as well as the plant genus containing most Antarctic endemic species. We applied phylogenetic and molecular dating methods based on nuclear ribosomal Internal Transcribed Spacer sequences, including all known Antarctic Schistidium species with available sample material. We additionally investigated the continent-wide genetic diversity within the most common Antarctic representative of the genus-the endemic species Schistidium antarctici-and performed preliminary phylogeographic analyses of the bipolar species Schistidium rivulare. Most previously described Antarctic Schistidium species were genetically distinct, confirming their specific status. Interspecific divergences of all species took place at least ~1 Mya, suggesting a likely in situ persistence in Antarctica for (at least) all endemic Schistidium species. The widespread endemic species, Schistidium antarctici, diverged from other Antarctic congeners in the late Miocene, thereby revealing the oldest extant plant species currently known in Antarctica, and providing increasing support for the hypothesis of vegetation survival through multiple glacial periods. Within S. antarctici we identified several distinct clades dividing the eastern Antarctic Peninsula and Scotia Arc islands from the western Antarctic Peninsula and all continental locations. This suggests that the mountainous spine on the Antarctic Peninsula forms a strong barrier to gene flow in this species, while increased genetic diversity in the northern Maritime Antarctic indicates likely glacial refugia in this area. This study provides an important first step toward assessing the diversity and evolutionary history of the most speciose moss genus in the Antarctic. The multi-million year presence of several endemic species contributes to studies on their adaptive potential to survive climate change over both historical and contemporary timescales.