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The present study aimed to evaluate the inflammatory response, oxidative status and fatty acid deposition in reproductive tissues of cats supplemented with the dried microalgae Schizochytrium spp. (Thraustochytriaceae) as a DHA source. Thirty-seven cats (males, n 21; females, n 16; 11·5 (sd 0·5) months of age) were divided by sex into five groups. Treatment diets contained algae biomass at 4·0, 8·0, 12·0 or 16·0 g/kg replacing poultry fat (n-6 source). Cats were fed the respective diet for 62 d and neutered on day 58. Blood samples were collected at the beginning of the experiment (day 1), before neutering (day 58) and 4 d after surgery (day 62) for analysis of inflammation and oxidative markers. Acute-phase protein levels were altered (P < 0·01) in the postoperative period, without any treatment effect (P > 0·05). PGE2 concentrations after surgery were reduced linearly (R2 0·8706; P = 0·002) with microalgal inclusion. Blood platelet count was reduced (P = 0·001) after the surgery regardless treatment, but it was higher in the DHA group compared with control (P < 0·001). The DHA deposition (testicles, R2 0·846; ovaries, R2 0·869) and the n-6:n-3 ratio (testicles, R2 0·859; ovaries, R2 0·955) in gonads had a pattern which fitted a quadratic model. DHA from Schizochytrium spp. modifies PGE2 response after the surgery in cats. The physiological roles of the DHA in the reproduction of cats were not investigated, but its gonadal deposition after supplementation was observed.

This work was carried out to study the nutritional quality of milk of cows fed palm oil (PAL) or coconut fat (COC), and the use of that milk as raw material for ice cream production. Three treatments were tested with 23 healthy cows: control (CON), PAL, and COC. The milk was collected at d 21 and 36 of the experimental diet. Proximate composition (moisture, ash, fat, protein, and carbohydrates) and fatty acid composition were evaluated on milk and ice cream, and sensorial analysis, color (lightness, green/red, and blue/yellow), overrun, and texture were evaluated on the ice cream. Fatty acids present in milk and ice cream were determined by gas chromatography. Sensory analysis results showed that the ice cream acceptability index was above 70%. No difference was observed for proximate composition in milk and ice cream. Chromatographic analysis showed an increase in saturated fatty acid concentration in CON and lower levels in PAL; polyunsaturated fatty acid concentration was higher in PAL and lower in CON, in milk and ice cream; monounsaturated fatty acid concentration in milk was higher in PAL and lower in CON but no difference was found in ice cream. Comparing n-3 content in milk and ice cream, we observed that PAL had higher levels than CON and COC. The results indicate that it is feasible to add sources of fat to the animal feed for fatty acid composition modulation of milk and ice cream.

O presente estudo avaliou a composição centesimal e o teor de ácidos graxos do tecido muscular do salmão (Salmo salar L.) sob duas formas: in natura e grelhado. Os resultados das análises físico-químicas dos fi lés de salmão (Salmo salar L.), em ambas as formas, mostraram que esses peixes apresentaram bom teor de proteínas e alto teor de gordura. Com relação ao perfi l de ácidos graxos, o salmão apresentou considerável índice de AGPI e, em especial os da série ômega-3 (n-3), mais especifi camente EPA e DHA. Apesar de os resultados da composição físico-química terem mostrado diferenças signifi cativas entre as formas analisadas, o aquecimento não interferiu nos percentuais de ácidos graxos. Assim, o salmão (salmo salar L.) pode ser favorável ao consumo humano, tanto na forma in natura quanto grelhado, contribuindo para uma dieta protéica, calórica e saudável.

This study evaluated the omega-3 (n-3) fatty acids and the proximate composition of muscle tissue of adult Nile tilapias to select the best feeding time length with a diet containing 70 (g kg−1 wt) flaxseed oil (FO). The results showed that dietary complementation with FO for 45 days is suitable for obtaining high levels of protein (164 g kg−1), total lipids (94 g kg−1), and ash (18 g kg−1). Furthermore, there was a significant difference (P

The objective of this study was to evaluate the influence of corn silage (CS) or sugarcane (SC) additions to finishing diets on voluntary intake, apparent digestibility, animal performance, and LM fatty acid (FA) composition in crossbred Holstein × Zebu bulls. Forty-two bulls (initial BW 328 ± 39.2 kg and an initial age of 23.5 mo) were used in this experiment. A completely randomized design was used to examine roughage source (CS vs. SC) and ratio of concentrate (CO) on nutrient utilization and production. The experimental diets consisted of 1) 60% CS and 40% CO on DM basis fed for 84 d (CS60:40), 2) 60% CS and 40% CO on DM basis fed for 42 d and 40% CS and 60% CO on DM basis fed for 42 d (CS reverse diet [CSR]), 3) 40% CS and 60% CO on DM basis fed for 84 d (CS40:60), 4) 60% SC and 40% CO on DM basis fed for 84 d (SC60:40), 5) 60% SC and 40% CO on DM basis fed for 42 d and 40% SC and 60% CO on DM basis fed for 42 d (SC reverse diet [SCR]), and 6) 40% SC and 60% CO on DM basis fed for 84 d (SC40:60). Dry matter and NDF intakes were greater (P0.01) in bulls fed CS-based diets than bulls fed SC-based diets. The greatest (P0.01) DM and CP digestibility were observed in bulls fed SC-based diets. Bulls fed CSR and CS40:60 had greater (P0.01) ADG than bulls fed SC-based diets. However, ADG was similar (P = 0.52) in bulls fed CS60:40, CSR, and SC40:60 diets. The percentage of C18:3 n-3 and C20:5 n-3 were greater (P0.01) in LM of bulls fed SC-based diets. The percentage of CLA was greater (P0.01) in LM of bulls fed SC60:40 than those fed CS-based diets. The findings of the present study indicate that SC40:60 can replace CS60:40 and CSR in finishing diets and, moreover, roughage source significantly altered the FA composition of crossbred Holstein × Zebu bulls LM.

The aim of this study was to investigate the effects on meat quality of crossbred bulls finished in a feedlot when some corn is replaced by glycerin and essential oils (EO) are added to the diet. Thirty bulls weighing 311 kg ± 28.8 kg (22 ± 2 months old) were randomly assigned to one of three diets: CON – Control (diet without glycerin and EO); GLY – 16.1% glycerin in dry matter (DM); and GEO – 16.1% glycerin in DM and EO (cashew: Anacardium occidentale; castor: Ricinus communis) at 442 mg/kg DM consumed. The bulls were kept in a feedlot for 115 days and slaughtered at an average weight of 467 ± 27.9 kg (25 ± 2 months old). The bulls were fed on sorghum silage, cracked corn, glycerin, soybean meal, urea, limestone, and mineral salt. Replacing corn with glycerin plus EO adding to the diet had no effect on the fat thickness, Longissimus muscle (LM) area, marbling, texture, colour, Warner–Bratzler shear force, or chemical composition of the LM. The fatty acid composition in the LM was similar among the diets, except for percentages of myristic and margaric acids. The diets had no effect on the percentage of saturated and polyunsaturated fatty acids. Animals fed on glycerin had a lower ratio of n-6 to n-3 in the LM when compared with the CON diet. Partial replacing of corn with glycerin and EO improved meat flavour, tenderness, and the preference of consumers. Corn may be replaced by glycerin to a 15% level of DM without any detrimental effect on the carcass characteristics or the chemical composition of the LM.

Camu-camu (Myrciaria dubia), a native fruit of the Amazon region, is one of the richest sources of vitamin C (2.4 to 3.0 g/100 g in the pulp) found in Brazil. The purpose of this work was the physical-chemical characterization of some nutrients and the valuation of vitamin C stability in stored camu-camu pulp, produced by the Agronomic Institute of Paraná (IAPAR), Paraná State, Brazil. The vitamin C determination was made by titration with potassium iodate. The fruit produced in Paraná State, presented a lower content of vitamin C than the one native of the amazon region, possibly due to the different development conditions of the plant, and consequently of the fruit, as well as the climatic variation, the humidity and the characteristics of the soil. Regarding the vitamin C stability in stored (-18 degrees C) camu-camu pulp, a considerable decrease in its concentration until the 28th day was observed lost 23% (from 1.57 to 1.21 g/100 g), staying approximately the same until the end of the experiment. After 335 days of storage, the content found was of approximately 1.16 g/100 g of pulp, the ascorbic acid losses amounted to 26%. This content was still higher than the one found for most fruits that are good sources of this vitamin.

The analyzed milk samples were collected from cross Saanen goats of different ages and different cross breeding types, throughout the lactation period, from September 1996 to December 1997. For the physical-chemical characteristics measured in this experiment, the following average values were obtained, followed by their respective standard deviations: pH (6.69 +/- 0.20); acidity (12.96 +/- 3.64 degrees D); density (1.030 +/- 0.009 mg.cm-3); fat (3.83 +/- 1.04%); crude protein (3.34 +/- 0.73%) and total solids (12.25 +/- 1.94 g.100 g-1). The lactation period influenced the values of acidity, fat, crude protein and total solids; these values decreased during the initial months and increased at the end of the lactation. The correlations were analyzed among the studied characteristics during the lactation, resulting in positive (p0.05) acidity/density correlation (r = 0.2115), stand out also the positive correlations (p0.01) among fat/total solids (r = 0.7715) and crude protein/total solids (r = 0.6228).

Total lipids from meat portions of breast, thigh, wing, side and back with and without skin from 10 roasted chickens were extracted with chloroform and methanol and gravimetrically determined, and their fatty acids were analysed as methyl esters by gaseous chromatography, using a flame ionization detector and capillary column. The main fatty acids found were: C16:0, C18:1 omega 9, and C18:2 omega 6. The average ratio observed between PUFA/SFA was of 0.98, mainly due to the great concentration of the C18:2 omega 6 fatty acid, with an average of 26.75%. Regarding to the lipids content, the skinless breast showed the lowest content, 0.78 g/100 g, while the back with skin was the one with the highest content, 12.13 g/100 g except for the pure skin, with 26.54 grams of lipids by 100 grams.

Ripe fruits of Barbados cherry Malpighia glabra L. proceeding from the fruit-growing section of Iguatemi Experimental Farm of Universidade Estadual de Maringa (PR), were triturated in a liquefier and hulled in a stainless steel sieve with 25 mesh. The bagasse (seeds and hull) was discarded and the vitamin C content was immediately determined, which was 1.79 g by 100 g of pulp. After that, the integral pulp was packed in glass flasks and submitted to the exhaustion and pasteurization processes and then hermetically closed. After the heat treatment the vitamin C content was 1.54 g by 100 g of pulp. The sealed flasks of Barbados cherry pulp, with and without the aluminum foil protection, were stored for 40 days. The first portion was kept at room temperature, the second in a refrigerator (1 degree C), and the third in a freezer (-18 degrees C). The vitamin C content analysis were realized on the 5th, 10th, 15th, 20th, 30th and 40th day. For the flasks stored without the aluminum foil protection, there was a loss of 22.08%, 7.79% and 1.30% and with aluminum foil the loss was of 10.40%, 3.90% and 1.30% for the storage at room, refrigeration and freezing temperatures, respectively. The results show that freezing method is the best form of vitamin C preservation.

Lipid and fatty acid levels in the edible flesh of 17 baked freshwater fish from Brazil's southern region were determined. Analyses of fatty acids methyl esters were performed by gas chromatography. Palmitic acid (C16:0) was the predominant saturated fatty acid, accouting for 50-70% of total saturated acids. Linoleic acid (C18:2 omega 6), linolenic acid (C18:3 omega 3), and docosahexaenoic acid (C22:6 omega 3) were the predominant polyunsatured fatty acids (PUFA). The data revealed that species such as barbado, corvina, pintado, and truta were good sources of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and that most freshwater fish examined were good sources of PUFA-omega 3.

article i nfo Article history: The impact of two factors, genotype (G) and treatment (raw or roasted peanut) (T), on the chemical composition of peanuts was studied using a chemometric method and Tukey's test. The peanut genotypes evaluated were cultivar cavalo vermelho (CCV), cultivar cavalo rosa (CCR) and cultivar tatu (CTA), in both raw and roasted states. The total lipid contents in the CTA and CCR peanuts were 40 and 45%, respectively. These values did not vary significantly (Pb0.05) after roasting. CCV had the greatest total lipid content, but it decreased significantly after roasting (from 50% to 45%). The variation in the percentage of lipids in the CCV and CCR genotypes was not significant, in contrast to the CTA genotype. The fatty acid (FA) 18:1n−9 predominated in the CCR and CCV samples (50%), without any difference between their raw genotypes. The values for FA 18:1n−9 were lower in the CTA peanut (40%). The second most abundant FA was 18:2n−6 (CCV=28%, CTA=38% and CCR=25%), followed by 16:0 (CCV and CCR=16% and CTA=11%). The other FAs found in the peanuts were 18:0, 20:0, 22:0, 24:0, 20:1n−9 and 18:3n−3. The contents of FAs 18:1n−9, 16:0, 20:0, and 20:1n−9 suffered significant reduction after roasting in all genotypes. ANOVA analysis of the influence of the main factors indicated that the contribution of the T variable for the majority of responses was low, being between 0.2 and 13%, except for FAs 16:0 and 18:3n−3 and for the saturated FA summations, which were 38, 60 and 22%, respectively. There was a significant contribution from the G factor for all responses, with values between 17 and 99%. The contribution of the interaction between the T and G factors was greater for the responses n6/n3 (56.6%) and for the FA 16:0 (23%). The other responses had values between 0.02 and 14%.

This study aimed to determine the fatty acid and chemical composition and cholesterol concentration of horsemeat, and to evaluate its taste acceptability by the Brazilian population. Horsemeat samples (M. longissimus dorsi) were obtained from a Parana State slaughterhouse. The chemical composition revealed a low lipid (2.9%) and high protein content (22.5%). The concentrations of the nutritionally important fatty acids, arachidonic acid (AA, 20:4n-6) and eicosapentaenoic acid (EPA, 20:5n-3), were 2.97% and 0.43%, respectively. The polyunsaturated fatty acid/saturated fatty acid (PUFA/SFA) ratio was 0.97, which is within the recommended range. The cholesterol concentration of 40.5 mg/100 g is lower than that of other meat such as chicken, beef, mutton and pork. The sensory evaluation revealed excellent acceptability. Keywords: Equus caballus, M. longissimus dorsi, PUFA/SFA, health food, meat quality