1. ‘Plantibodies’: a flexible approach to design resistance against pathogens
- Author
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Fred J. Gommers, Arjen Schots, J. F. Zil Verentant, H. Pomp, L. Bouwman-Smits, Jaap Bakker, J.M. de Boer, Jan Roosien, A. Schouten, Bertanne Visser, Willem J. Stiekema, and Hein Overmars
- Subjects
biology ,medicine.drug_class ,Plant Science ,Horticulture ,Monoclonal antibody ,scFv ,single chain antibodies ,Microbiology ,Antigen ,Immunology ,biology.protein ,medicine ,Plantibody ,Monoclonal antibodies ,Laboratory of Nematology ,Antibody ,potato cyst nematodes ,Laboratorium voor Nematologie ,Agronomy and Crop Science ,Gene ,Pathogen ,Function (biology) ,Single-Chain Antibodies - Abstract
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.
- Published
- 1992