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1. Comparison of the response of mammary gland tissue from two divergent lines of goat with high and low milk somatic cell scores to an experimental Staphylococcus aureus infection

Author

Rachel Rupp, Rossana Capoferri, Alessandra Stella, Federica Riva, John L. Williams, Giuliano Pisoni, Paolo Moroni, J. Filipe, Paola Cremonesi, Paola Roccabianca, Bianca Castiglioni, Marcello Del Corvo, Istituto Spallanzani, National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Università degli Studi di Milano = University of Milan (UNIMI), Università cattolica del Sacro Cuore [Piacenza e Cremona] (Unicatt), University of Adelaide, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Cornell University [New York], SELMOL, UE0332, OSMOY, European Project: 506416,FOOD-CT- 2004- 506416, Consiglio Nazionale delle Ricerche (CNR), University of Milan, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Spallanzani Istituto, and Partenaires INRAE

Subjects
Somatic cell count, Staphylococcus aureus, Neutrophils, 040301 veterinary sciences, Somatic cell, Immunology, Mammary gland, Cell Count, Mastitis, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Culling, Breeding, Biology, medicine.disease_cause, Cell Line, 0403 veterinary science, 03 medical and health sciences, Mammary Glands, Animal, Immune system, medicine, Animals, Mammary tissue, Udder, 030304 developmental biology, Inflammation, 2. Zero hunger, 0303 health sciences, Goat Diseases, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, General Veterinary, Gene Expression Profiling, Goats, Inflammatory response, 04 agricultural and veterinary sciences, Lipid Metabolism, medicine.disease, Immunity, Innate, Milk, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, medicine.anatomical_structure, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Goat, Female, Transcriptome
Abstract

International audience; Mastitis represents one of the major economic and health threats to the livestock sector associated with reduction in milk quality, loss of production and is a major reason for culling. Somatic cell score (SCS) is used as a criterion in breeding programmes to select cows genetically less susceptible to mastitis. The relevance of SCS as a predictor of udder health and susceptibility to mastitis is still untested in goats. In this study, two lines of French Alpine goats selected for extreme breeding values for somatic cell scores, one line with high SCS (HSCS) and the other with low SCS (LSCS), were used to test the hypothesis that the mammary response and function differed between the lines. The aim of the present study was to investigate differences in the early immune response in caprine mammary gland tissues challenged with Staphylococcus aureus, one of the main pathogens responsible for the intra-mammary infection in small ruminants, using transcriptomic and histopathology analyses.The comparison between HSCS and LSCS goat lines, showed differences in the response at the histological level for inflammation, presence of neutrophils and micro-abscess formation, and at the molecular level in the expression of CXCL8, IL-6, NFKBIZ and IL-1 beta. CXCL8 and CXCL2 genes, which showed a higher level of expression in the experimentally infected HSCS line. The molecular data and histopathology both suggested that following S. aureus infection, mobilization, recruitment, infiltration, and chemotaxis of neutrophil, leads to a more severe inflammation in the HSCS compared to LSCS animals. Our results represent an initial basis for further studies to unravel the genetic basis of early mastitis inflammatory responses and the selection of dairy animals more resistant to bacterial mastitis.

Published
2021

2. Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E coli LPS challenge

Author

Gilles Charpigny, M. Chanrot, Luigi Bonizzi, Alessio Soggiu, Patrice Humblot, Cristian Piras, Andrea Urbani, Viviana Greco, Paola Roncada, Yongzhi Guo, Dipartimento di Medicina Veterinaria, Università degli studi di Milano [Milano], Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Rajamangala University of Technology, Proteomics and Metabonomics Unit, IRCCS Santa Lucia Foundation, Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Istituto Sperimentale Italiano Lazzaro Spallanzani, Rajamangala University of Te chnology Srivijaya (RMUTSV, Thailand), European Project: 311776, Dipartimento di Medicina Veterinaria (DIMEVET), Università degli Studi di Milano [Milano] (UNIMI), Università cattolica del Sacro Cuore [Milano] (Unicatt), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Istituto Spallanzani, Università degli Studi di Milano = University of Milan (UNIMI), Rajamangala University of Technology Thanyaburi (RMUTT), and École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)

Subjects
Lipopolysaccharides, Proteomics, 0301 basic medicine, mammary gland, bovin, Proteome, Lipopolysaccharide, Workflow, chemistry.chemical_compound, Protein Interaction Mapping, Gene expression, Protein Interaction Maps, endometrium, [SDV.BDD]Life Sciences [q-bio]/Development Biology, 2. Zero hunger, uterine disease, Biologie du développement, cellule épithéliale, Development Biology, 3. Good health, endomètre, Female, medicine.symptom, escherichia coli, Bacterial outer membrane, Metabolic Networks and Pathways, Biotechnology, gene expression, ovarian function, Cell Survival, Inflammation, Biology, 03 medical and health sciences, medicine, Matrix-Assisted Laser Desorption-Ionization, Animals, Settore BIO/10 - BIOCHIMICA, Molecular Biology, Spectrometry, Cell growth, epithelial cell, Epithelial Cells, Mass, Molecular biology, In vitro, 030104 developmental biology, chemistry, Apoptosis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Immunology, Cattle, Energy Metabolism
Abstract

E. coli is one of the most frequently involved bacteria in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria involved in pathogenic processes leading to post-partum metritis and endometritis in cattle. It also causes inflammation of the endometrium. The increase of cell proliferation by LPS is part of the inflammatory process. The aim of this study was to investigate possible changes in protein expression in relation to the proliferative response of bEECs after challenge with E. coli-LPS. In vitro culture of bEECs was performed from cow genital tracts collected at a slaughterhouse. In passage 5, bEECs from each of 9 cows (3 series of 3 cows) were exposed to 0, 8, and 16 μg ml(-1) LPS for 72 h. At time 0 and 72 h later, attached cells/living cells were counted and for each time and LPS dosage, cells were frozen for proteomic analyses. All samples from the 3 series were analyzed by 2-D gel electrophoresis coupled to MALDI-TOF/TOF mass spectrometry. The samples from the first series were subjected to shotgun nLC-MS/MS analysis. From the whole differential proteomics analysis, 38 proteins were differentially expressed (p < 0.05 to p < 0.001) following exposure to LPS. Among them, twenty-eight were found to be up-regulated in the LPS groups in comparison to control groups and ten were down-regulated. Differentially expressed proteins were associated with cell proliferation and apoptosis, transcription, destabilization of cell structure, oxidative stress, regulation of histones, allergy and general cell metabolism pathways. The de-regulations induced by LPS were consistent with the proliferative phenotype and indicated strong alterations of several cell functions. In addition, some of the differentially expressed proteins relates to pathways activated at the time of implantation. The specific changes induced through those signals may have negative consequences for the establishment of pregnancy.

Published
2017

3. An alternative experimental case-control design for genetic association studies on bovine mastitis

Author

John L. Williams, Giulietta Minozzi, A. Pedretti, Stefano Biffani, Giulio Pagnacco, Francis Minvielle, Mario Luini, M. Del Corvo, Rossana Capoferri, Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche [Roma] (CNR), Parco Tecnologico Padano Srl, Insituto Sperimentale Italiano ‘Lazzaro Spallanzani’, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, School of Animal and Veterinary Sciences, Faculty of Sciences, University of Adelaide, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna 'Bruno Ubertini' (IZSLER), and Istituto Spallanzani

Subjects
0301 basic medicine, experimental design, [SDV]Life Sciences [q-bio], Genome-wide association study, Single-nucleotide polymorphism, association study, mastitis, SF1-1100, Polymorphism, Single Nucleotide, 03 medical and health sciences, Animals, Medicine, Mastitis, Bovine, Genetic Association Studies, Genetic association, Genetics, business.industry, Sire, 0402 animal and dairy science, 04 agricultural and veterinary sciences, medicine.disease, 040201 dairy & animal science, Animal culture, Mastitis, 030104 developmental biology, Italy, Genetic marker, cattle, Case-Control Studies, Cohort, genetic markers, Herd, Female, Animal Science and Zoology, genetic marker, business
Abstract

The possibility of using genetic control strategies to increase disease resistance to infectious diseases relies on the identification of markers to include in the breeding plans. Possible incomplete exposure of mastitis-free (control) animals, however, is a major issue to find relevant markers in genetic association studies for infectious diseases. Usually, designs based on elite dairy sires are used in association studies, but an epidemiological case-control strategy, based on cows repeatedly field-tested could be an alternative for disease traits. To test this hypothesis, genetic association results obtained in the present work from a cohort of Italian Holstein cows tested for mastitis over time were compared with those from a previous genome-wide scan on Italian Holstein sires genotyped with 50k single nucleotide polymorphisms for de-regressed estimated breeding values for somatic cell counts (SCCs) on Bos taurus autosome (BTA6) and BTA14. A total of 1121 cows were selected for the case-control approach (cases=550, controls=571), on a combination of herd level of SCC incidence and of within herd individual level of SCC. The association study was conducted on nine previously identified markers, six on BTA6 and four on BTA14, using the R statistical environment with the 'qtscore' function of the GenABEL package, on high/low adjusted linear score as a binomial trait. The results obtained in the cow cohort selected on epidemiological information were in agreement with those obtained from the previous sire genome-wide association study (GWAS). Six out of the nine markers showed significant association, four on BTA14 (rs109146371, rs109234250, rs109421300, rs109162116) and two on BTA6 (rs110527224 and rs42766480). Most importantly, using mastitis as a case study, the current work further validated the alternative use of historical field disease data in case-control designs for genetic analysis of infectious diseases in livestock.

Published
2017

4. Metapopulation patterns of additive and nonadditive genetic variance in the sea bass (Dicentrarchus labrax)

Author

Mathilde Dupont-Nivet, Béatrice Chatain, Pierrick Haffray, Hervé Chavanne, Marc Vandeputte, Bruno Guinand, Alain Vergnet, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD [France-Ouest]), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Istituto Sperimentale Italiano Lazzaro Spallanzani, Guinand, Bruno, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Syndicats des sélectionneurs avicoles et aquacoles français (SYSAAF), Istituto Spallanzani, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), and École Pratique des Hautes Études (EPHE)

Subjects
0106 biological sciences, 0301 basic medicine, additive and nonadditive variance, mito-nuclear epistasis, mito‐nuclear epistasis, Darwin's corollary, [SDV]Life Sciences [q-bio], Population, Introgression, Metapopulation, Biology, 010603 evolutionary biology, 01 natural sciences, Diallel cross, 03 medical and health sciences, Hybrid zone, Genetic variation, pleiotropy, heterosis, 14. Life underwater, Sea bass, education, Ecology, Evolution, Behavior and Systematics, Original Research, Nature and Landscape Conservation, education.field_of_study, Ecology, Reproductive isolation, 030104 developmental biology, Evolutionary biology
Abstract

Ajouter clé UT WOS; Describing and explaining the geographic within-species variation in phenotypes ("phenogeography") in the sea over a species distribution range is central to our understanding of a variety of eco-evolutionary topics. However, phenogeographic studies that have a large potential to investigate adaptive variation are overcome by phylogeographic studies, still mainly focusing on neutral markers. How genotypic and phenotypic data could covary over large geographic scales remains poorly understood in marine species. We crossed 75 noninbred sires (five origins) and 26 dams (two origins; each side of a hybrid zone) in a factorial diallel cross in order to investigate geographic variation for early survival and sex ratio in the metapopulation of the European sea bass (Dicentrarchus labrax), a highly prized marine fish species. Full-sib families (N = 1,950) were produced and reared in a common environment. Parentage assignment of 7,200 individuals was performed with seven microsatellite markers. Generalized linear models showed significant additive effects for both traits and pleiotropy between traits. A significant nonadditive genetic effect was detected. Different expression of traits and distinct relative performances were found for reciprocal crosses involving populations located on each side of the main hybrid zone located at the Almeria-Oran front, illustrating asymmetric reproductive isolation. The poor fitness performance observed for the Western Mediterranean population of sea bass is discussed as it represents the main source of seed hatchery production, but also because it potentially illustrates nonadaptive introgression and maladaptation.

Published
2017

5. Changes in protein expression profiles in bovine endometrial epithelial cells (bEEC) following E. Coli lipopolysaccharide challenge

Author

Guo, Y. Z., Piras, C., Soggiu, A., Chanrot, M., Båge, R., Andersson, G., Reinaud, Pierrette, Charpigny, Gilles, Sandra, Olivier, Valarcher, J.F., Roncada, P., HUMBLOT, P., Department of clinical sciences, Swedish University of Agricultural Sciences (SLU), Università degli Studi di Milano [Milano] (UNIMI), Rajamangala University of Technology Lanna, Partenaires INRAE, Department of Animal Bredding and Genetics, Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Department of Virology Immunobiology and Parasitology, National Veterinary Institute, Istituto Spallanzani, Research was partially funded by RUMSTV (Thailand), International Embryo Transfer Society (IETS). USA., and Istituto Sperimentale Italiano Lazzaro Spallanzani

Subjects
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

6. The effect of interspecific oocytes on demethylation of sperm DNA

Author

Grazyna Ptak, Cesare Galli, Adrian Bird, Jane Taylor, Lorraine E. Young, Richard R. Meehan, John Gardner, Michelle McGarry, Pasqualino Loi, Nathalie Beaujean, Giovanna Lazzari, Ian Wilmut, BEAUJEAN N, TAYLOR JE, MCGARRY M, GARDNER JO, WILMUT I, LOI P, PTAK G, GALLI C., LAZZARI G, BIRD A, YOUNG LE, MEEHAN RR, Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), The Roslin Institute, Universita degli studi di Teramo - University of Teramo [Italie] (UNITE), Istituto Spallanzani, Wellcome Trust Centre for Cell Biology, University of Edinburgh, Institute of Genetics and Division of Obstetrics and Gynaecology ( University of Nottingham), Medical Research Council, School of Chemistry, University of Edinburgh, Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), and Istituto Sperimentale Italiano Lazzaro Spallanzani

Subjects
Male, [SDV]Life Sciences [q-bio], medicine.medical_treatment, Intracytoplasmic sperm injection, Mice, chemistry.chemical_compound, 0302 clinical medicine, Demethylase activity, [SDV.BDD]Life Sciences [q-bio]/Development Biology, reproductive and urinary physiology, GENE-EXPRESSION, 0303 health sciences, Microscopy, Confocal, 030219 obstetrics & reproductive medicine, Multidisciplinary, PATERNAL GENOME, Gene Expression Regulation, Developmental, CELL NUCLEAR TRANSFER, Biological Sciences, Cell cycle, Spermatozoa, Chromatin, Cell biology, DNA methylation, MAMMALIAN DEVELOPMENT, Female, endocrine system, Biology, DONOR GENOME, 03 medical and health sciences, medicine, Animals, Sperm Injections, Intracytoplasmic, 030304 developmental biology, Demethylation, METHYLATION PATTERNS, Sheep, urogenital system, CLONED BOVINE EMBRYOS, ABERRANT METHYLATION, MOUSE EMBRYO, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, DNA, DNA Methylation, Molecular biology, Sperm, chemistry, PREIMPLANTATION DEVELOPMENT, Oocytes
Abstract

International audience; In contrast to mice, in sheep no genome-wide demethylation of the paternal genome occurs within the first postfertilization cell cycle. This difference could be due either to an absence of a sheep demethylase activity that is present in mouse ooplasm or to an increased protection of methylated cytosine residues in sheep sperm. Here, we use interspecies intracytoplasmic sperm injection to demonstrate that sheep sperm DNA can be demethylated in mouse oocytes. Surprisingly, mouse sperm can also be demethylated to a limited extent in sheep oocytes. Our results suggest that the murine demethylation process is facilitated either by a sperm-derived factor or by male pronuclear chromatin composition.

Published
2004

7. Multi-site evaluation of the rearing performances of 5 wild populations of European sea bass (Dicentrarchus labrax)

Author

Mathilde Dupont-Nivet, R. Ricoux, Carlos Mazorra, Pierrick Haffray, Pedro Marques, Stanislas Laureau, Glen Pagelson, Marc Vandeputte, Romain Garouste, Béatrice Chatain, Tetsuzan Benny Ron, Marta Gameiro, Hervé Chavanne, Alain Vergnet, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Intensification raisonnée et écologique pour une pisciculture durable (UMR INTREPID), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Istituto Sperimentale Italiano Lazzaro Spallanzani, Ecloserie Marine de Gravelines, National Center for Mariculture, Israel Oceanographic and Limnological Research, Department of Human Nutrition, Food & Animal Sciences, CTAHR, University of Hawaii, Ardag Red Sea Mariculture (ARDAG), Tinamenor S.A, Les Poissons du Soleil, Foros de Galeado, Viveiro Vilanova S.A., This work is part of the COMPETUS project, funded by Ardag Red Sea Mariculture (Eilat, Israel), Ecloserie Marine de Gravelines (Gravelines, France), Les Poissons du Soleil (Balaruc, France), Tinamenor SA (Pesues, Cantabria, Spain), Viveiro Vila Nova (Vila Nova de Milfontes, Portugal) and the European Union (project COOP-CT-2005-017633). It is also part of the program of the Research Group 'Fish Genetic Improvement' between INRA and Ifremer., Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA)-IFR140, and Istituto Spallanzani

Subjects
Mediterranean climate, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Genotype by environment interaction, Artificial fertilization, growth, Population, european sea bass, Population genetics, Zoology, Growth, Aquatic Science, genotype by environment interaction, Diallel cross, Aquaculture, Dicentrarchus labrax, 14. Life underwater, European sea bass, Sea bass, education, selective breeding, Processing yields, education.field_of_study, biology, business.industry, Ecology, processing yield, biology.organism_classification, Selective breeding, Dicentrarchus, business, dicentrarchus labrax
Abstract

Chantier qualité GA; International audience; Sea bass is a major species in Mediterranean aquaculture, but has a distribution area ranging from North Atlantic to South Mediterranean, with a population structure previously revealed by population genetics. To test the farming performances of wild sea bass populations, we produced a partial diallel cross mating scheme, using sires originating from North Atlantic (NAT), South Atlantic (SAT), West Mediterranean (WEM), North-East Mediterranean (NEM) and South-East Mediterranean (SEM). Fifteen sires per origin were mated in a full-factorial design using artificial fertilization with 9 NAT dams and 17 WEM dams, producing 10 population crosses and 1950 potential full-sib families. All fish were reared together, then tagged at an average weight of 20 g and distributed to four different sites (1800 fish per site). They were grown to an objective of 200 g mean weight, where 737 to 775 fish were slaughtered in each site, and their parentage was recovered using 6 to 7 microsatellite loci, resulting in 98.9% unique assignments. All populations had similar growth rates until tagging size (20 g), but differences appeared later on. No heterosis appeared for growth rate, and genotype by environment interaction (G × E) at the population level was limited, with a significant re-ranking only in one rearing site, while strong G × E for growth rate was observed within populations. Populations were different in shape, muscular fat content, and carcass yield, but not in fillet yield. In general, heterosis was absent and G × E was very limited between populations. No "ideal" population combining all favorable traits was identified. Differences between extreme populations ranged between 3 and 49% of the mean, depending on the traits. Interestingly, in almost all cases, these differences were within the reach of one generation of intense (5%) phenotypic selection.

Published
2014

8. Domestication and selection for growth induce a genetic sex-ratio shift towards females in a European sea bass (Dicentrarchus labrax) population

Author

Vandeputte, Marc, Dupont-Nivet, Mathilde, Chavanne, Hervé, Haffray, Pierrick, Quillet, Edwige, Chatain, Béatrice, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Istituto Sperimentale Lazzaro Spallanzani, Localita La Quercia, Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), and Istituto Spallanzani

Subjects
domestication, sélection génétique, analyse de croissance, [SDV]Life Sciences [q-bio], sex ratio, dicentrarchus labrax, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2012

9. Response of the goat mammary gland to infection with Staphylococcus aureus revealed by gene expression profiling in milk somatic and white blood cells

Author

Rachel Rupp, Rossana Capoferri, Francesco Strozzi, Paola Cremonesi, Giuliano Pisoni, John L. Williams, Marcello Del Corvo, Alessandra Stella, Paola Modesto, Hugues Caillat, Bianca Castiglioni, Paolo Moroni, Consiglio Nazionale delle Ricerche (CNR), Istituto Sperimetnale Italiano L. Spallanzani, Partenaires INRAE, Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, University of Milan, Parco Tecnologico Padano, Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Università degli Studi di Milano [Milano] (UNIMI), Istituto Zooprofilattico Sperimentale del Piemonte, Quality Milk Production Services, Cornell University [New York], Istituto Spallanzani, and Capoferri, Rossana

Subjects
Chemokine, Somatic cell, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Gene Expression, Mastitis, experimental challenge, Gene expression, goat mammary gland, Leukocytes, 0303 health sciences, biology, somatic cell count, Goats, goat, 04 agricultural and veterinary sciences, Staphylococcal Infections, mastitis, transcriptome, Serum Amyloid P-Component, Cytokine, C-Reactive Protein, Milk, Cytokines, Female, Research Article, Biotechnology, Staphylococcus aureus, lcsh:QH426-470, lcsh:Biotechnology, Somatic Cells Score, Microbiology, 03 medical and health sciences, Immune system, Mammary Glands, Animal, lcsh:TP248.13-248.65, medicine, Genetics, gene expression profiling, Animals, Humans, 030304 developmental biology, Goat Diseases, 0402 animal and dairy science, Lipid metabolism, Lipid Metabolism, S. aureus, 040201 dairy & animal science, Fold change, Gene expression profiling, lcsh:Genetics, Immunology, biology.protein
Abstract

Background S. aureus is one of the main pathogens responsible for the intra-mammary infection in dairy ruminants. Although much work has been carried out to understand the complex physiological and cellular events that occur in the mammary gland in response to S. aureus, the protective mechanisms are still poorly understood. The objectives of the present study were to investigate gene expression during the early response of the goat mammary gland to an experimental challenge with S. aureus, in order to better understand the local and systemic response and to compare them in two divergent lines of goat selected for high and low milk somatic cell scores. Results No differences in gene expression were found between high and low SCS (Somatic Cells Score) selection lines. Analysing the two groups together, an expression of 300 genes were found to change from T0 before infection, and T4 at 24 hours and T5 at 30 hours following challenge. In blood derived white blood cells 8 genes showed increased expression between T0 and T5 and 1 gene has reduced expression. The genes showing the greatest increase in expression following challenge (5.65 to 3.16 fold change) play an important role in (i) immune and inflammatory response (NFKB1, TNFAIP6, BASP1, IRF1, PLEK, BATF3); (ii) the regulation of innate resistance to pathogens (PTX3); and (iii) the regulation of cell metabolism (CYTH4, SLC2A6, ARG2). The genes with reduced expression (−1.5 to −2.5 fold) included genes involved in (i) lipid metabolism (ABCG2, FASN), (ii) chemokine, cytokine and intracellular signalling (SPPI), and (iii) cell cytoskeleton and extracellular matrix (KRT19). Conclusions Analysis of genes with differential expression following infection showed an inverse relationship between immune response and lipid metabolism in the early response of the mammary gland to the S. aureus challenge. PTX3 showed a large change in expression in both milk and blood, and is therefore a candidate for further studies on immune response associated with mastitis.

Published
2012
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10. Genotype x environment interactions for growth rate in European sea bass (Dicentrarchus labrax)

Author

Vandeputte, Marc, Dupont-Nivet, Mathilde, Haffray, Pierrick, Vergnet, Alain, Chavanne, Hervé, Laureau, Stanislas, Chatain, Béatrice, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Istituto Spallanzani, Ecloserie Marine de Gravelines, and Istituto Sperimentale Lazzaro Spallanzani, Localita La Quercia

Subjects
facteur de croissance, [SDV]Life Sciences [q-bio], intéraction génotype environnement, dicentrarchus labrax, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

11. Genetic and environmental influences on shape variation in the European sea bass (Dicentrarchus labrax)

Author

Costa, Corrado, Vandeputte, Marc, Antonucci, Francesca, Boglione, Clara, Menesatti, Paolo, Cenadelli, Silvia, Parati, Katia, Chavanne, Hervé, Chatain, Béatrice, Agricultural Engineering Research Unit, Agricultural Research Council (CRA), Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Station d’aquaculture expérimentale, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratory of Experimental Ecology and Aquaculture, Department. Biology, Università degli Studi di Roma Tor Vergata [Roma], Istituto Sperimentale Italiano Lazzaro Spallanzani, Institut National de la Recherche Agronomique (INRA)-AgroParisTech, and Istituto Spallanzani

Subjects
Dicentrarchus labrax, ecomorphology, genetic variation, geometric morphometry, heritability, image analysis, Settore BIO/07, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], dicentrarchus labrax
Abstract

Chantier qualité GA; This paper demonstrates the contribution of both genetic and environmental effects on cultured European sea bass shape. We used the progeny of five populations of sea bass, in a partly diallel design, to investigate the genetics of shape (estimated with geometric morphometrics) in European sea bass. This was done using a common garden experiment with microsatellite markers assignment to parents and populations to avoid confusion between genetic and environmental effects. Additionally, one of the populations was studied over four different aquaculture facilities to investigate the effects of environment on shape. For the first time in this species, shape-related traits were linked with genetic variation. The first relative warp analysis axis clearly differentiated rearing sites, demonstrating that the main shape/weight effects are related to culturing conditions, thereby accounting for ecomorphologically related differences. The second axis strongly differentiated groups by parental origins; there was a good correlation between shape differences and geographic distances between broodstock sampling locations. High heritabilities of axes scores (0.40–0.55) showed high genetic variation for shape within populations. This study shows that variation in shape has a high genetic component in sea bass, both at the population level and within populations.

Published
2010

12. Effets de la domestication et de la sélection chez le bar

Author

Chatain, Béatrice, Dupont-Nivet, Mathilde, Porte, Jean-Denis, Aupérin, Benoît, Vergnet, Alain, Vidal, Marie-Odile, Chavanne, Hervé, Laureau, Stanislas, Haffray, Pierrick, Bégout, Marie-Laure, Vandeputte, Marc, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Istituto Spallanzani, Ecloserie Marine de Gravelines, Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Centre de Recherche sur les Ecosystèmes Littoraux Anthropisés (CRELA), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Istituto Sperimentale Lazzaro Spallanzani, Localita La Quercia, Syndicats des sélectionneurs avicoles et aquacoles français (SYSAAF), and Centre National de la Recherche Scientifique (CNRS)-Université de La Rochelle (ULR)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
Domestication, [SDV]Life Sciences [q-bio], Poisson, Selection, Bar
Abstract

absent

Published
2009

13. Processing quality of selected fish: quasi-absence of correlated responses to selection for growth in the brown trout [i]Salmo trutta[/i] and in the sea bass [i]Dicentrarchus labrax[/i]

Author

Vandeputte, Marc, Bugeon, Jérôme, Dupont-Nivet, Mathilde, Chavanne, H., Haffray, Pierrick, Lefèvre, Florence, Labbé, Laurent, Vergnet, A., Chatain, B., Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Chemin de Maguelone, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), Localita La Quercia, Istituto Sperimentale Italiano Lazzaro Spallanzani, INRA SCRIBE Campus de Beaulieu, Syndicats des sélectionneurs avicoles et aquacoles français (SYSAAF), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Istituto Spallanzani, and Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF)

Subjects
salmonid, genetics, [SDV.BDD]Life Sciences [q-bio]/Development Biology
Abstract

absent

Published
2009

14. Pas de modification de qualité technologique chez les poissons sélectionnés pour la croissance : exemples de la truite fario et du bar

Author

Vandeputte, Marc, Bugeon, Jérôme, Dupont-Nivet, Mathilde, Chavanne, H., Haffray, Pierrick, Lefèvre, Florence, Labbé, Laurent, Vergnet, A., Chatain, B., Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Chemin de Maguelone, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Istituto Spallanzani, Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Ouest Genopole, Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), Istituto Sperimentale Lazzaro Spallanzani, Localita La Quercia, Station INRA SCRIBE Campus de Beaulieu, Syndicats des sélectionneurs avicoles et aquacoles français (SYSAAF), and AgroParisTech-Institut National de la Recherche Agronomique (INRA)

Subjects
GENETIQUE ANIMALE, TRANSFORMATION DES POISSONS, [SDV]Life Sciences [q-bio], SLECTION, CROISSANCE, BAR COMMUN, SALMONIDE
Abstract

absent

Published
2009

15. Amélioration génétique du bar : performances en élevage de souches sauvages, réponses directes et corrélées à la sélection croissance

Author

Vandeputte, Marc, Dupont-Nivet, Mathilde, Porte, Jean-Denis, Garouste, Romain, Millot, Sandie, Aupérin, Benoît, Bégout, Marie-Laure, Vergnet, Alain, Vidal, Marie-Odile, Chavanne, Hervé, Ricoux, Rémi, Pagelson, Glen, Marques, Pedro, Mazzora, Carlos, Laureau, Stanislas, Haffray, Pierrick, Chatain, Béatrice, Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Centre de Recherche sur les Ecosystèmes Littoraux Anthropisés (CRELA), Centre National de la Recherche Scientifique (CNRS)-Université de La Rochelle (ULR)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Istituto Sperimentale Lazzaro Spallanzani, Localita La Quercia, Les Poissons du Soleil, Ardag Red Sea Mariculture (ARDAG), Viveiro Vilanova S.A., Tinamenor S.A, Ecloserie Marine de Gravelines, Syndicats des sélectionneurs avicoles et aquacoles français (SYSAAF), UE projet CRAFT Competus, Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Istituto Spallanzani, and Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF)

Subjects
[SDV]Life Sciences [q-bio], Sélection, Amélioration génétique, Poisson, Croissance, Bar
Abstract

absent

Published
2009

16. Investigating fish reproduction in Europe Reprofish

Author

Maybank, Miranda, Canario, Adelino, Carnevali, Oliana, Carrillo, Manuel, Cerda, Joan, Chavanne, Hervé, Fostier, Alexis, Guiguen, Yann, Hall, Timothy J., Harache, Yves, Kah, Olivier, Labbé, Catherine, Lareyre, Jean-Jacques, Linhart, Otomar, Mananos Sanchez, Evaristo, Migaud, Hervé, Muñoz-Cueto, José Antonio, Murgat, Vincent, Mylonas, Dinos, Pottinger, Tom, Schulz, Rüdiger, Taranger, Geir Lasse, Tyler, Charles, van den Thillart, Guido, Williot, Patrick, Zanuy, Silvia, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), University of Algrave, Partenaires INRAE, Università Politecnica delle Marche, Institute of Aquaculture Torre la Sal (IATS), Institute of Agrifood Research and Technology (IRTA), Spallanzani Istituto, DG-Research, European Central Bank, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), CNRS UMR6026 Interactions cellulaires et moléculaires (ICM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), University of South Bohemia, Institute of Aquaculture, University of Cadiz, Pisciculture Charles Murgat, Hellenic Center for Marine Research (HCMR), Natural Environment Research Council (NERC), Utrecht University [Utrecht], Norwegian Institute of Marine Research, University of Exeter, Leiden University, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), European Commission. Sixth Framework Programme, Istituto Spallanzani, Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology (IRTA), Interactions cellulaires et moléculaires (ICM), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Hellenic Centre for Marine Research (HCMR), and Universiteit Leiden

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]
Abstract

The Reprofish working group has published a special booklet for the benefit of the aquaculture sector (stakeholder, fish farmers…), for the students, and for the public. This booklet provides a simplified overview of the knowledge on the reproductive biology of the major farmed fish species in Europe. In addition, it illustrates how basic and applied researches are used to improve the profit of the fish farms and to maintain the quality of products. Particularly, the use of biotechnological tools that allow one to control the sex ratio, to induce sterility, to delay/advance puberty, or to induce spawning, is introduced and discussed. Finally, the booklet presents other issues such as the sustainability of the aquaculture or the fish as a model animal for human frontier research.

Published
2009

17. Response to domestication and selection for growth in the European sea bass (Dicentrarchus labrax) in separate and mixed tanks

Author

Katia Parati, Béatrice Chatain, Silvia Cenadelli, Alain Vergnet, Mathilde Dupont-Nivet, Pierrick Haffray, Marie-Odile Vidal, Hervé Chavanne, Marc Vandeputte, Unité de recherche Génétique des Poissons (UGP), Institut National de la Recherche Agronomique (INRA), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Istituto Spallanzani, Instituto Sperimentale Lazzaro Spallanzani, and Partenaires INRAE

Subjects
Serranidae, Rearing, [SDV]Life Sciences [q-bio], animal diseases, Parentage assignment, Growth, Domestication, Realized heritability, realized heritability, fluids and secretions, Aquaculture, groupers and fairy basslets] [Serranidae [Sea basses], selective breeding, media_common, 0303 health sciences, biology, parentage assignment, food and beverages, 04 agricultural and veterinary sciences, Nephrocalcinosis, Dicentrarchus labrax [Bass], Dicentrarchus, dicentrarchus labrax, competition, microsatellite, growth, media_common.quotation_subject, european sea bass, Aquatic Science, Selective breeding, Competition (biology), 03 medical and health sciences, Animal science, nephrocalcinosis, Dicentrarchus labrax, Selection response, 14. Life underwater, European sea bass, Sea bass, Microsatellites, 030304 developmental biology, Competition, Growth rate, business.industry, Sire, technology, industry, and agriculture, Heritability, biology.organism_classification, Fishery, Fertilization, 040102 fisheries, 0401 agriculture, forestry, and fisheries, business, selection response
Abstract

Selective breeding of European sea bass (Dicentrarchus labrax) receives a growing interest, as the estimated heritability of growth is medium to high. In this study, we compared the offspring of four groups of sea bass sires, mated with the same wild dams: wild (W), first generation of domestication (D), first generation of mass selection for length (M), first generation of PROSPER-like selection for length (P). The comparison was done both in replicated tanks (separate rearing) and in mixed tanks (mixed rearing) where sire origins were recovered by genotyping of eight microsatellite markers. Weight, length and growth rate were measured from day 238 post-fertilization (69 g mean weight) to day 611 post-fertilization (390 g mean weight). Both in mixed and separate tanks. both selected groups (P, M) were larger than unselected groups (W D). No difference was seen at any time between Wand D, nor between M and P. The selection response estimate on weight was larger in mixed tanks when compared to separate tanks (+42% in mixed tanks, +23% in separate tanks at day 611), yielding realized heritability estimates of 0.60 and 0.34, respectively, and confirming the excellent potential of the species for growth improvement through selective breeding. Both selection response and the amplification effect between mixed and separate tanks decreased as rearing density increased. Our hypothesis is that selection response is magnified by competition in mixed tanks, while suboptimal rearing conditions lower the observed selection response, more in separate tanks (where selected thus larger fish are at a higher density than unselected ones) than in mixed tanks (where all fish experience the same density effects). (c) 2008 Elsevier B.V. All rights reserved.

Published
2009

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