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The effect of interspecific oocytes on demethylation of sperm DNA
- Source :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2004, 101 (20), pp.7636-7640. ⟨10.1073/pnas.0400730101⟩
- Publication Year :
- 2004
- Publisher :
- Proceedings of the National Academy of Sciences, 2004.
-
Abstract
- International audience; In contrast to mice, in sheep no genome-wide demethylation of the paternal genome occurs within the first postfertilization cell cycle. This difference could be due either to an absence of a sheep demethylase activity that is present in mouse ooplasm or to an increased protection of methylated cytosine residues in sheep sperm. Here, we use interspecies intracytoplasmic sperm injection to demonstrate that sheep sperm DNA can be demethylated in mouse oocytes. Surprisingly, mouse sperm can also be demethylated to a limited extent in sheep oocytes. Our results suggest that the murine demethylation process is facilitated either by a sperm-derived factor or by male pronuclear chromatin composition.
- Subjects :
- Male
[SDV]Life Sciences [q-bio]
medicine.medical_treatment
Intracytoplasmic sperm injection
Mice
chemistry.chemical_compound
0302 clinical medicine
Demethylase activity
[SDV.BDD]Life Sciences [q-bio]/Development Biology
reproductive and urinary physiology
GENE-EXPRESSION
0303 health sciences
Microscopy, Confocal
030219 obstetrics & reproductive medicine
Multidisciplinary
PATERNAL GENOME
Gene Expression Regulation, Developmental
CELL NUCLEAR TRANSFER
Biological Sciences
Cell cycle
Spermatozoa
Chromatin
Cell biology
DNA methylation
MAMMALIAN DEVELOPMENT
Female
endocrine system
Biology
DONOR GENOME
03 medical and health sciences
medicine
Animals
Sperm Injections, Intracytoplasmic
030304 developmental biology
Demethylation
METHYLATION PATTERNS
Sheep
urogenital system
CLONED BOVINE EMBRYOS
ABERRANT METHYLATION
MOUSE EMBRYO
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
DNA
DNA Methylation
Molecular biology
Sperm
chemistry
PREIMPLANTATION DEVELOPMENT
Oocytes
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 101
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....29663ec5d27890c10a77b093e09da5a4