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1. Comparative analysis of the perception of nuclear risk in two populations (expert/non-expert) in France

Author: Christophe Den Auwer, Sandra Russo, Sandra Perez, Nicolas Pottier, Hervé Michel, Cyril Drouot, Maria Rosa Beccia, Damienne Provitolo, Geoges Carle, Denis Josse, Audrey Leriche, Gaëlle Creff, Nicolas Glaichenhaus, Fréderic Castagnola, Franck Fiorelli, Pascale Steichen, Thierry Pourcher, Études des Structures, des Processus d’Adaptation et des Changements de l’Espace (ESPACE), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Avignon Université (AU)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Institut de Chimie de Nice (ICN), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Transporteurs et Imagerie, Radiothérapie en Oncologie et Mécanismes biologiques des Altérations du Tissu Osseux (TIRO-MATOs - UMR E4320), Service Hospitalier Frédéric Joliot (SHFJ), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-UMR E4320 (TIRO-MATOs), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA), Groupe de Recherche en Droit, Economie et Gestion (GREDEG), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Transitions : savoirs, médias, territoires (URE) (//Transitions), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Dipartimento di Chimica e Chimica Industriale, Università di Pisa, Service départemental d’incendie et de secours de Loir-et-Cher (SDIS), Service Départemental d’Incendie et de Secours 50, Service Départemental d’Incendie et de Secours des Alpes-Maritimes, Service de Santé et de Secours Médical, Nice, Centre expert MADoPA, Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), SDIS06-Service Départemental d'Incendies et de Secours des Alpes Maritimes, Université Côte d'Azur (UCA), Géoazur (GEOAZUR 7329), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire de la Côte d'Azur, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), CODIREM, ANR-15-IDEX-0001,UCA JEDI,Idex UCA JEDI(2015), Université Côte d'Azur (UCA)-Avignon Université (AU)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Université Nice Sophia Antipolis (... - 2019) (UNS), UMR E4320 (TIRO-MATOs), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Côte d'Azur (UCA)-Service Hospitalier Frédéric Joliot (SHFJ), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Perez, Sandra, Idex UCA JEDI - - UCA JEDI2015 - ANR-15-IDEX-0001 - IDEX - VALID, Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)
Subjects: Risk, Forms of energy, [SHS.GEO] Humanities and Social Sciences/Geography, 020209 energy, Energy (esotericism), media_common.quotation_subject, Population, 02 engineering and technology, 7. Clean energy, Training (civil), 020401 chemical engineering, Order (exchange), Perception, 0202 electrical engineering, electronic engineering, information engineering, Social media, 0204 chemical engineering, Marketing, education, media_common, Nuclear risk, education.field_of_study, [SHS.SOCIO]Humanities and Social Sciences/Sociology, Perspective (graphical), Nuclear energy, [SHS.GEO]Humanities and Social Sciences/Geography, [SDE.ES]Environmental Sciences/Environmental and Society, Ecological transition, General Energy, Geography, [SDE.ES] Environmental Sciences/Environmental and Society, lcsh:Electrical engineering. Electronics. Nuclear engineering, [CHIM.RADIO]Chemical Sciences/Radiochemistry, lcsh:TK1-9971
Abstract: Presentation of the article in video : https://vimeo.com/465124099; International audience; In an effort to reduce CO2 emissions, many countries throughout the world are initiating plans to transition to more sustainable forms of energy. Nuclear energy would appear to be a powerful contender to replace fossil fuels, or at least be an unavoidable option, from an energy-mix perspective. However, nuclear energy suffers from a poor image among certain populations, especially the young, who favor the development of renewable energies. We wanted to get a more accurate read of what was happening in France, one of the most nuclearized countries in the world, where the share of nuclear energy is greater than 70%. Since plans are underway to reduce this level to 50% by 2035, understanding popular perceptions on this matter is even more important. In order to gain a better understanding, we interviewed an “expert” population and compared the results obtained with a so-called young “non-expert” student population. The first group is composed of firefighters, about half of whom have attended training in radiological risks, and the other, a non-expert population, composed of students from Université Côte d’Azur (UCA), who are potentially influenced (for better or worse) by social media. The aim of this study is to compare and contrast any differences in perception that these two distinct populations may have on the subject of nuclear energy.
Published: 2020
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2. Correction: Stratification and prediction of remission in first-episode psychosis patients: the OPTiMiSE cohort study

Author: Richard Drake, Nicolas Glaichenhaus, Susana Barbosa, Laetitia Davidovic, René S. Kahn, Lionel Fillatre, Marion Leboyer, Dan Rujescu, Shôn Lewis, Stefan Leucht, Celso Arango, Stéphane Jamain, Olfa Khalfallah, Douglas Daoudlarian, Rejane Troudet, Guillaume Fond, Iris E. C. Sommer, Cyprien Gilet, Birte Glenthøj, Robert H. Yolken, Philip McGuire, W. Wolfgang Fleischhacker, Paola Dazzan, Emanuela Martinuzzi, Covadonga M. Díaz-Caneja, Wafa Bel Haj Ali, Inge Winter, Diabète de Type 1 : mécanismes et traitements immunologiques, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), Laboratoire Modélisation et Sûreté des Systèmes (LM2S), Institut Charles Delaunay (ICD), Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Aix Marseille Université (AMU), Assistance Publique - Hôpitaux de Marseille (APHM), Brain Centre Rudolf Magnus [Utrecht], University Medical Center [Utrecht], Psychological Medicine, Dept. of Psychiatry, Adolescent Unit, Hospital General Universitario Gregorio Marañón, Division of Psychiatric Genomics, Icahn School of Medicine at Mount Sinai [New York] (MSSM), Stanley Laboratory of Developmental Neurovirology, Johns Hopkins University Medical Center, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Université Nice Sophia Antipolis (... - 2019) (UNS), and Assistance Publique-Hôpitaux de Marseille (AP-HM)
Subjects: Pediatrics, medicine.medical_specialty, business.industry, 05 social sciences, Stratification (mathematics), 03 medical and health sciences, Cellular and Molecular Neuroscience, Psychiatry and Mental health, 0302 clinical medicine, First episode psychosis, [SDV.MHEP.PSM]Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, 0502 economics and business, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, Medicine, 050211 marketing, business, 030217 neurology & neurosurgery, Biological Psychiatry, ComputingMilieux_MISCELLANEOUS, Cohort study
Abstract: The original Article did not feature the list of collaborators. This has now been corrected in the PDF and HTML versions of this Article.
Published: 2019
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3. The Patient-Reported Experience Measure for Improving qUality of care in Mental health (PREMIUM) project in France: study protocol for the development and implementation strategy

Author: Martinuzzi, Emanuela, Barbosa, Susana, Daoudlarian, Douglas, Bel Haj Ali, Wafa, Gilet, Cyprien, Fillatre, Lionel, Khalfallah, Olfa, Troudet, Réjane, Jamain, Stéphane, Fond, Guillaume, Sommer, Iris, Leucht, Stefan, Dazzan, Paola, McGuire, Philip, Arango, Celso, Diaz-Caneja, Covadonga, Fleischhacker, Wolfgang, Rujescu, Dan, Glenthøj, Birte, Winter, Inge, Kahn, René Sylvain, Yolken, Robert, Lewis, Shon, Drake, Richard, Davidovic, Laetitia, Leboyer, Marion, Glaichenhaus, Nicolas, Laboratoire de Santé Publique, Aix Marseille Université (AMU), Assistance Publique - Hôpitaux de Marseille (APHM), Service d'Hépato-Gastroentérologie [CHU Rouen], Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Aix-Marseille Sciences Economiques (AMSE), École des hautes études en sciences sociales (EHESS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Pôle de psychiatrie, Assistance Publique - Hôpitaux de Marseille (APHM)-Hôpital Sainte-Marguerite [CHU - APHM] (Hôpitaux Sud )-CHU Marseille, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg (UNISTRA), Fondation FondaMental [Créteil], Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Neuro-Psycho Pharmacologie des Systèmes Dopimanégiques sous-corticaux (NPsy-Sydo), CHU Clermont-Ferrand-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Géographie-cités (GC (UMR_8504)), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Centre d'études et de recherche sur les services de santé et la qualité de vie (CEReSS), Diabète de Type 1 : mécanismes et traitements immunologiques, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Laboratoire d'Informatique, Signaux, et Systèmes de Sophia-Antipolis (I3S) / Equipe IMAGES-CREATIVE, Signal, Images et Systèmes (Laboratoire I3S - SIS), Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), Laboratoire Modélisation et Sûreté des Systèmes (LM2S), Institut Charles Delaunay (ICD), Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Troyes (UTT), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Assistance Publique-Hôpitaux de Marseille (AP-HM), Brain Centre Rudolf Magnus [Utrecht], University Medical Center [Utrecht], Psychological Medicine, Dept. of Psychiatry, Adolescent Unit, Hospital General Universitario Gregorio Marañón, Division of Psychiatric Genomics, Icahn School of Medicine at Mount Sinai [New York] (MSSM), Stanley Laboratory of Developmental Neurovirology, Johns Hopkins University Medical Center, COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA), Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Charles Nicolle [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Institut de Recherche et Documentation en Economie de la Santé (IRDES), Institut de la Recherche et Documentation en Economie de la Santé, Hôpital Charles Nicolle [Rouen]-Université de Rouen Normandie (UNIROUEN), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), Université Côte d'Azur (UCA), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS), Montpellier 1 - UFR de Médecine (UM1 Médecine), Université Montpellier 1 (UM1), Santé Publique et maladies Chroniques : Qualité de vie Concepts, Usages et Limites, Déterminants (SPMC), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM), and CHU Clermont-Ferrand-Université d'Auvergne - Clermont-Ferrand I (UdA)
Subjects: computerized adaptive testing, media_common.quotation_subject, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Item bank, Medicine (miscellaneous), PARIS team, Study Protocol, 03 medical and health sciences, 0302 clinical medicine, item bank, Nursing, quality of care, Patient experience, Health care, Medicine, Quality (business), 030212 general & internal medicine, COM, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), ComputingMilieux_MISCELLANEOUS, media_common, Protocol (science), [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, business.industry, Health Policy, [SCCO.NEUR]Cognitive science/Neuroscience, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, AD, Mental health, psychiatry, 3. Good health, 030227 psychiatry, Patient Preference and Adherence, [SDV.MHEP.PSM]Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, patient-reported experience measures, Computerized adaptive testing, business, Social Sciences (miscellaneous), Qualitative research
Abstract: Sara Fernandes,1 Guillaume Fond,1 Xavier Zendjidjian,1 Pierre Michel,1 Karine Baumstarck,1 Christophe Lancon,1 Fabrice Berna,2 Franck Schurhoff,2 Bruno Aouizerate,2 Chantal Henry,2 Bruno Etain,2 Ludovic Samalin,2 Marion Leboyer,2 Pierre-Michel Llorca,2 Magali Coldefy,3 Pascal Auquier,1 LaurentBoyer1 On behalf of the French PREMIUM Group 1Aix-Marseille University, School of Medicine, CEReSS – Health Service Research and Quality of Life Center – EA 3279 Research Unit, Marseille, France; 2FondaMental Foundation, Créteil, France; 3Institute for Research and Information in Health Economics (IRDES), Paris, France Background: Measuring the quality and performance of health care is a major challenge in improving the efficiency of a health system. Patient experience is one important measure of the quality of health care, and the use of patient-reported experience measures (PREMs) is recommended. The aims of this project are 1) to develop item banks of PREMs that assess the quality of health care for adult patients with psychiatric disorders (schizophrenia, bipolar disorder, and depression) and to validate computerized adaptive testing (CAT) to support the routine use of PREMs; and 2) to analyze the implementation and acceptability of the CAT among patients, professionals, and health authorities.Methods: This multicenter and cross-sectional study is based on a mixed method approach, integrating qualitative and quantitative methodologies in two main phases: 1) item bank and CAT development based on a standardized procedure, including conceptual work and definition of the domain mapping, item selection, calibration of the item bank and CAT simulations to elaborate the administration algorithm, and CAT validation; and 2) a qualitative study exploring the implementation and acceptability of the CAT among patients, professionals, and health authorities.Discussion: The development of a set of PREMs on quality of care in mental health that overcomes the limitations of previous works (ie, allowing national comparisons regardless of the characteristics of patients and care and based on modern testing using item banks and CAT) could help health care professionals and health system policymakers to identify strategies to improve the quality and efficiency of mental health care.Trial registration: NCT02491866. Keywords: patient-reported experience measures, quality of care, item bank, computerized adaptive testing, psychiatry
Published: 2019
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4. Une approche bioculturelle du premier aliment du nouveau-né. Le colostrum

Author: Joël Candau, Eve Bureau-Point, Karine Durand, Céline Geffroy, Véronique Ginouvès, Marie-Luce Gélard, Arnaud Halloy, Estelle Herrscher, Hicham Khabbache, Hans Hahn, P., Ludovic Kibora, Kathrin Knödel, Maria-Leticia Mazzucchi-Ferreira, Thierry Rosso, Benoist Schaal, Leang Kruy Sim, Isabelle Séguy, Celine Verguet, Valérie Verhasselt, Jean-Pierre Zirotti, Laboratoire d'Anthropologie et de Psychologie Cliniques, Cognitives et Sociales (LAPCOS), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Maison méditerranéenne des sciences de l'Homme (MMSH), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Centre d'anthropologie culturelle (CANTHEL - EA 4545), Université Paris Descartes - Paris 5 (UPD5), Laboratoire méditerranéen de préhistoire Europe-Afrique (LAMPEA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), Goethe-University Frankfurt am Main, Centre National de la Recherche Scientifique et Technique, Goethe-Universität Frankfurt am Main, Universidade Federal de Pelotas = Federal University of Pelotas (UFPel), Service Gynécologie-Obstétrique, Calmette Hospital [Phnom Penh], Centre d'Études Préhistoire, Antiquité, Moyen-Age (CEPAM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Immunologie des maladies infectieuses allergiques et autoimmunes, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), ANR-12-BSH1-0008, Estelle Herrscher, Isabelle Séguy, ANR-12-BSH1-0008,COLOSTRUM,L'alimentation pré-lactée (don et consommation néonatale du colostrum) : pratiques, représentations et enjeux de santé publique.(2012), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS), Centre Norbert Elias (CNELIAS), École des hautes études en sciences sociales (EHESS)-Avignon Université (AU)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA), Calmette Hospital, Phnom Penh, Cambodge, Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)
Subjects: nourrisson, colostrum, open science, pluridisciplinarité, ANR, health, pre-milk newborn feeding, [SHS.ANTHRO-SE]Humanities and Social Sciences/Social Anthropology and ethnology, santé, anthropologie
Abstract: International audience; The "Colostrum" project, sponsored by the National Research Agency (ANR) from 2013 to 2016, is an interdisciplinary and international programme entirely dedicated to pre-milk newborn feeding. It concerns both the representations and practices around the donation of colostrum by the mother and its consumption by the child. It brought together 12 partners in 7 countries (Germany, Bolivia, Brazil, Burkina Faso, Cambodia, France, Morocco) spread over 4 continents, and the research itself includes four complementary components: anthropological, immunological, psychobiological, as well as an open science component for the restitution of results. The first part of the article displays the context of this research program, while the second part reveals the problem and the anthropological, immunological and psychobiological aspects of the program. The third and last part presents the first results, followed by general observations.; Le projet « Colostrum » soutenu par l’Agence Nationale de la Recherche (ANR) de 2013 à 2016 est un programme interdisciplinaire et international entièrement consacré à l’alimentation pré-lactée du nouveau-né. Il concerne aussi bien les représentations et pratiques autour du don de colostrum par la mère que sa consommation par l’enfant. Il a réuni 12 partenaires dans 7 pays (Allemagne, Bolivie, Brésil, Burkina Faso, Cambodge, France, Maroc) répartis sur 4 continents.La recherche proprement dite comprend quatre volets complémentaires : anthropologique, immunologique, psychobiologique, ainsi qu’un volet open science pour la restitution des résultats. La première partie de l'article présente le contexte dans lequel s’inscrit ce programme de recherche tandis que la deuxième détaille la problématique et les volets anthropologique, immunologique et psychobiologique du programme. La troisième et dernière partie livre les premiers résultats, puis conclue par des remarques de portée générale.
Published: 2019

5. In The Quest For Stokes V - Science Cases And Technical Challenges

Author: Wiesemeyer, Helmut, Agudo, I., Baudry, Alain, Downes, D., Güsten, R., Herpin, Fabrice, Houde, M., Kreysa, E., Maury, A., Menten, K., Morris, D., Navarro, S., Paubert, G., Pillai, T., Thum, C., Weiß, A., Institut de RadioAstronomie Millimétrique (IRAM), Centre National de la Recherche Scientifique (CNRS), FORMATION STELLAIRE 2017, Laboratoire d'Astrophysique de Bordeaux [Pessac] (LAB), Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Max Planck Institute for Radio Astronomy, Aquatic Contaminant Research Division, Environment and Climate Change Canada, Max-Planck-Institut für Radioastronomie (MPIFR), Astrophysique Interprétation Modélisation (AIM (UMR_7158 / UMR_E_9005 / UM_112)), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7), RedZinc Services Ltd.[Dublin], Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Physique, Albert-Ludwigs-Universität Freiburg, Astrophysique Interprétation Modélisation (AIM (UMR7158 / UMR_E_9005 / UM_112)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Astrophysics::Solar and Stellar Astrophysics, [SDU.ASTR.GA]Sciences of the Universe [physics]/Astrophysics [astro-ph]/Galactic Astrophysics [astro-ph.GA], Astrophysics::Galaxy Astrophysics
Abstract: International audience; Circular polarization is produced either in the emission process through the Zeeman effect, or through the transfer of polarized radiation in a magneto-active medium. While the former mechanism provides a direct access to the strength of the magnetic field along the sightline, the conversion of linear to circular polarization reveals particular magnetic field structures or magnetospheric events. In the first part I will present selected science cases. While Stokes V may be strong in OH masers forming in supernova remnants, the Zeeman effect becomes weaker in masers associated with star-forming environments or circumstellar envelopes. As masers probe restricted physical conditions, the Zeeman effect in thermal lines is a valuable analysis tool, but even more difficult to measure. In particular, the instrumental leakage from linear to circular polarization frequently exceeds the intrinsic Stokes V. This statement also holds for the Faraday conversion in AGN. A correction for instrumental polarization across the field of view is thus essential, but hindered by the lack of suitable calibrators. In the second part I will propose calibration schemes for both the image and UV plane.
Published: 2017
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6. Engraftment and in vivo proliferation advantage of gene-corrected mobilized CD34(+) cells from Fanconi anemia patients

Author: Parinda A. Mehta, Julián Sevilla, Maria Roser Pujol, José A. Casado, Anne Galy, Sabine Charrier, María L. Lamana, Rebeca Sanchez-Dominguez, Cristina Díaz de Heredia, Jordi Surrallés, Rosa Yañez, Guillermo Guenechea, José C. Segovia, Susana Navarro, Juan A. Bueren, Paula Río, Independent, Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Indiana University Cyclotron Facility (IUCF), Indiana University [Bloomington], Indiana University System-Indiana University System, Barenbrug, Approches génétiques intégrées et nouvelles thérapies pour les maladies rares (INTEGRARE), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Généthon, Universidad Mayor de San Andrés (UMSA), Université Nice Sophia Antipolis (... - 2019) (UNS), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Généthon-École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)
Subjects: 0301 basic medicine, Myeloid, [SDV]Life Sciences [q-bio], Immunology, CD34, Genetic Therapy/*methods, Biology, Biochemistry, 03 medical and health sciences, Mice, Transduction, 0302 clinical medicine, Genetic Vectors, CD34/immunology, Fanconi Anemia/pathology/*therapy, medicine, Autologous transplantation, Humans, Animals, Progenitor cell, Antigens, Child, Preschool, Hematopoietic Stem Cell Mobilization, Hematopoietic Stem Cells/pathology, Lentivirus/genetics, Genetic/*methods, Graft Survival, Hematopoietic stem cell, Cell Biology, Hematology, 3. Good health, Transplantation, Fanconi Anemia Complementation Group C Protein/*genetics, Haematopoiesis, 030104 developmental biology, medicine.anatomical_structure, Hematopoietic Stem Cell Transplantation/*methods, 030220 oncology & carcinogenesis, Heterografts
Abstract: International audience; Previous Fanconi anemia (FA) gene therapy studies have failed to demonstrate engraftment of gene-corrected hematopoietic stem and progenitor cells (HSPCs) from FA patients, either after autologous transplantation or infusion into immunodeficient mice. In this study, we demonstrate that a validated short transduction protocol of G-CSF plus plerixafor-mobilized CD34(+) cells from FA-A patients with a therapeutic FANCA-lentiviral vector corrects the phenotype of in vitro cultured hematopoietic progenitor cells. Transplantation of transduced FA CD34(+) cells into immunodeficient mice resulted in reproducible engraftment of myeloid, lymphoid, and CD34(+) cells. Importantly, a marked increase in the proportion of phenotypically corrected, patient-derived hematopoietic cells was observed after transplantation with respect to the infused CD34(+) graft, indicating the proliferative advantage of corrected FA-A hematopoietic repopulating cells. Our data demonstrate for the first time that optimized protocols of hematopoietic stem cell collection from FA patients, followed by the short and clinically validated transduction of these cells with a therapeutic lentiviral vector, results in the generation of phenotypically corrected HSPCs capable of repopulating and developing proliferation advantage in immunodeficient mice. Our results suggest that clinical approaches for FA gene therapy similar to those used in this study will facilitate hematopoietic repopulation in FA patients with gene corrected HSPCs, opening new prospects for gene therapy of FA patients.
Published: 2017
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7. Splenic CD8α+ dendritic cells undergo rapid programming by cytosolic bacteria and inflammation to induce protective CD8+ T-cell memory

Author: Saidi M.Homa Soudja, Grégoire Lauvau, Laura Campisi, Nicolas Glaichenhaus, Frédéric Brau, Anne Lazzari, Frederic Geissmann, Emilie Narni-Mancinelli, Julie Cazareth, Delphine Bassand, Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Centre for Cellular and Molecular Biology of Inflammation, King‘s College London, Institut de pharmacologie moléculaire et cellulaire (IPMC), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Department of Microbiology and Immunology, and Albert Einstein College of Medicine [New York]
Subjects: MESH: Inflammation, Adoptive cell transfer, MESH: Spleen, Priming (immunology), Cell Count, MESH: T-Lymphocyte Subsets, MESH: Virulence, CD8-Positive T-Lymphocytes, MESH: Listeria monocytogenes, Lymphocyte Activation, MESH: Membrane Transport Proteins, Mice, Cytosol, 0302 clinical medicine, MESH: Cytosol, T-Lymphocyte Subsets, Immunology and Allergy, Cytotoxic T cell, MESH: Animals, Listeriosis, MESH: Bacterial Proteins, Cells, Cultured, Sequence Deletion, Adenosine Triphosphatases, Mice, Inbred BALB C, 0303 health sciences, MESH: Dendritic Cells, Virulence, biology, MESH: Sequence Deletion, Acquired immune system, MESH: CD8-Positive T-Lymphocytes, Adoptive Transfer, 3. Good health, MESH: Immunologic Memory, [SDV.IMM]Life Sciences [q-bio]/Immunology, medicine.symptom, MESH: Cells, Cultured, CD8 Antigens, Immunology, MESH: Mice, Inbred BALB C, Inflammation, 03 medical and health sciences, Bacterial Proteins, Antigen, MHC class I, MESH: Adenosine Triphosphatases, medicine, Animals, MESH: Lymphocyte Activation, MESH: Mice, 030304 developmental biology, SecA Proteins, MESH: Cell Count, Membrane Transport Proteins, Dendritic Cells, Listeria monocytogenes, MESH: Adoptive Transfer, MESH: Listeriosis, biology.protein, MESH: Antigens, CD8, Immunologic Memory, SEC Translocation Channels, Spleen, CD8, 030215 immunology
Abstract: International audience; Memory CD8(+) T lymphocytes are critical effector cells of the adaptive immune system mediating long-lived pathogen-specific protective immunity. Three signals - antigen, costimulation and inflammation - orchestrate optimal CD8(+) T-cell priming and differentiation into effector and memory cells and shape T-cell functional fate and ability to protect against challenge infections. While among the conventional spleen DCs (cDCs), the CD8α(+) but not the CD8α(-) cDCs most efficiently mediate CD8(+) T-cell priming, it is unclear which subset, irrespective of their capacity to process MHC class I-associated antigens, is most efficient at inducing naïve CD8(+) T-cell differentiation into pathogen-specific protective memory cells in vivo. Moreover, the origin of the required signals is still unclear. Using mice infected with the intracellular bacterium Listeria monocytogenes, we show that splenic CD8α(+) cDCs become endowed with all functional features to optimally prime protective memory CD8(+) T cells in vivo within only a few hours post-immunization. Such programming requires both cytosolic signals resulting from bacterial invasion of the host cells and extracellular inflammatory mediators. Thus, these data designate these cells as the best candidates to facilitate the development of cell-based vaccine therapy.
Published: 2011
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8. Stem Cell Gene Therapy for Fanconi Anemia: Report from the 1st International Fanconi Anemia Gene Therapy Working Group Meeting

Author: Juan A. Bueren, Susana Navarro, Jennifer E. Adair, Paula Río, Michael Antoniou, Cynthia C. Bartholomae, Julián Sevilla, Pamela S. Becker, Anne Galy, Adrian J. Thrasher, Bruce R. Blazar, David A. Williams, Marina Cavazzana-Calvo, Thomas Carroll, Luigi Naldini, Robert Dalgleish, Els Verhoeyen, Helmut Hanenberg, Raffaele Renella, H. Bobby Gaspar, D. Wade Clapp, Dirk Lindeman, Hans-Peter Kiem, Jakub Tolar, John E. Wagner, Manfred Schmidt, Christof von Kalle, Tolar, J, Adair, Je, Antoniou, M, Bartholomae, Cc, Becker, P, Blazar, Br, Bueren, J, Carroll, T, Cavazzana Calvo, M, Clapp, Dw, Dalgleish, R, Galy, A, Gaspar, Hb, Hanenberg, H, Von Kalle, C, Kiem, Hp, Lindeman, D, Naldini, Luigi, Navarro, S, Renella, R, Rio, P, Sevilla, J, Schmidt, M, Verhoeyen, E, Wagner, Je, Williams, Da, Thrasher, Aj, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Immunologie moléculaire et biothérapies innovantes (IMBI), Généthon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Évry-Val-d'Essonne (UEVE)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Virus enveloppés, vecteurs et immunothérapie – Enveloped viruses, Vectors and Immuno-therapy (EVIR), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Généthon, Université Nice Sophia Antipolis (1965 - 2019) (UNS), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Oncology, medicine.medical_specialty, Stem Cell Transplantation/methods, Genetic enhancement, [SDV]Life Sciences [q-bio], Review, Genetic Therapy/*methods, Gene product, 03 medical and health sciences, 0302 clinical medicine, Fanconi anemia, Internal medicine, Drug Discovery, medicine, Genetics, Humans, Gene, Molecular Biology, 030304 developmental biology, Pharmacology, 0303 health sciences, Hematopoietic Stem Cells/cytology, Fanconi Anemia/*therapy, business.industry, Genetic Therapy, Congresses as Topic, medicine.disease, Hematopoietic Stem Cells, 3. Good health, Clinical trial, Transplantation, Fanconi Anemia, 030220 oncology & carcinogenesis, Immunology, Savior sibling, Molecular Medicine, Stem cell, business, Stem Cell Transplantation
Abstract: Survival rates after allogeneic hematopoietic cell transplantation (HCT) for Fanconi anemia (FA) have increased dramatically since 2000. However, the use of autologous stem cell gene therapy, whereby the patient's own blood stem cells are modified to express the wild-type gene product, could potentially avoid the early and late complications of allogeneic HCT. Over the last decades, gene therapy has experienced a high degree of optimism interrupted by periods of diminished expectation. Optimism stems from recent examples of successful gene correction in several congenital immunodeficiencies, whereas diminished expectations come from the realization that gene therapy will not be free of side effects. The goal of the 1st International Fanconi Anemia Gene Therapy Working Group Meeting was to determine the optimal strategy for moving stem cell gene therapy into clinical trials for individuals with FA. To this end, key investigators examined vector design, transduction method, criteria for large-scale clinical-grade vector manufacture, hematopoietic cell preparation, and eligibility criteria for FA patients most likely to benefit. The report summarizes the roadmap for the development of gene therapy for FA.
Published: 2011
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9. A subset of dendritic cells induces CD4+ T cells to produce IFN-γ by an IL-12–independent but CD70-dependent mechanism in vivo

Author: Hideo Yagita, Evelyne Mougneau, Helena Soares, Olga Mizenina, Diana Dudziak, Michel C. Nussenzweig, Nicholas Glaichenhaus, Ralph M. Steinman, HaeNa Waechter, Laboratory of Cellular Physiology and Immunology and Chris Browne Center, Rockefeller University [New York], Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Immunology, Juntendo University School of Medicine, and Laboratory of Molecular Immunology and the Howard Hughes Institute
Subjects: MESH: Interleukin-12, MESH: Signal Transduction, CD4-Positive T-Lymphocytes, Helper T lymphocyte, Protozoan Proteins, MESH: Mice, Knockout, Mice, 0302 clinical medicine, Immunology and Allergy, MESH: Animals, Interferon gamma, MESH: Protozoan Proteins, MESH: Antigens, CD, MESH: Receptors, Cell Surface, Mice, Knockout, Antigen Presentation, Mice, Inbred BALB C, 0303 health sciences, MESH: Dendritic Cells, MESH: CD4-Positive T-Lymphocytes, MESH: Enzyme-Linked Immunosorbent Assay, Cell Differentiation, Articles, Interleukin-12, Cell biology, Interleukin 12, [SDV.IMM]Life Sciences [q-bio]/Immunology, Signal Transduction, medicine.drug, MESH: Cell Differentiation, MESH: Interferon Type II, MESH: Lymphocyte Subsets, Immunology, Antigen presentation, MESH: Mice, Inbred BALB C, Antigens, Protozoan, Enzyme-Linked Immunosorbent Assay, Receptors, Cell Surface, Biology, Article, Minor Histocompatibility Antigens, Interferon-gamma, 03 medical and health sciences, Antigen, Antigens, CD, medicine, Animals, Lectins, C-Type, Antigen-presenting cell, MESH: Mice, 030304 developmental biology, T-cell receptor, Dendritic Cells, Dendritic cell, Molecular biology, Lymphocyte Subsets, MESH: Antigens, CD70, MESH: Antigen Presentation, MESH: Lectins, C-Type, MESH: Antigens, Protozoan, CD27 Ligand, 030215 immunology
Abstract: Interferon (IFN)-gamma, a cytokine critical for resistance to infection and tumors, is produced by CD4(+) helper T lymphocytes after stimulation by cultured dendritic cells (DCs) that secrete a cofactor, interleukin (IL)-12. We have identified a major IL-12-independent pathway whereby DCs induce IFN-gamma-secreting T helper (Th)1 CD4(+) T cells in vivo. This pathway requires the membrane-associated tumor necrosis family member CD70 and was identified by targeting the LACK antigen from Leishmania major within an antibody to CD205 (DEC-205), an uptake receptor on a subset of DCs. Another major DC subset, targeted with 33D1 anti-DCIR2 antibody, also induced IFN-gamma in vivo but required IL-12, not CD70. Isolated CD205(+) DCs expressed cell surface CD70 when presenting antigen to T cell receptor transgenic T cells, and this distinction was independent of maturation stimuli. CD70 was also essential for CD205(+) DC function in vivo. Detection of the IL-12-independent IFN-gamma pathway was obscured with nontargeted LACK, which was presented by both DC subsets. This in situ analysis points to CD70 as a decision maker for Th1 differentiation by CD205(+) DCs, even in Th2-prone BALB/c animals and potentially in vaccine design. The results indicate that two DC subsets have innate propensities to differentially affect the Th1/Th2 balance in vivo and by distinct mechanisms.
Published: 2007
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10. Ongoing and prolonged response in adult low-grade gliomas treated with radiotherapy

Author: Karima Mokhtari, M. Sanson, Caroline Houillier, Khê Hoang-Xuan, Yannick Marie, Agusti Alentorn, Dimitri Psimaras, Gentian Kaloshi, Soledad Navarro, Jean-Yves Delattre, François Ducray, Ahmed Idbaih, L. Dainese, Benjamin Ribba, Blandine Boisselier, Centre de recherche en neurosciences de Lyon - Lyon Neuroscience Research Center (CRNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Numerical Medicine (NUMED), Unité de Mathématiques Pures et Appliquées (UMPA-ENSL), École normale supérieure de Lyon (ENS de Lyon)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Centre National de la Recherche Scientifique (CNRS)-Inria Grenoble - Rhône-Alpes, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de recherche en neurosciences de Lyon (CRNL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Inria Grenoble - Rhône-Alpes, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Unité de Mathématiques Pures et Appliquées (UMPA-ENSL), Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), UPMC - Département de neurologie 2 - Mazarin, Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)
Subjects: Adult, Male, Cancer Research, medicine.medical_specialty, Time Factors, Adolescent, Growth kinetics, medicine.medical_treatment, Urology, 03 medical and health sciences, Young Adult, 0302 clinical medicine, medicine, Overall survival, Humans, RANO Criteria, P53 expression, neoplasms, Aged, Retrospective Studies, medicine.diagnostic_test, Tumor size, business.industry, Brain Neoplasms, Magnetic resonance imaging, Glioma, Middle Aged, Magnetic Resonance Imaging, [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation, 3. Good health, Surgery, Radiation therapy, Survival Rate, Treatment Outcome, Neurology, Oncology, 030220 oncology & carcinogenesis, Initial phase, Disease Progression, Female, Neurology (clinical), Neoplasm Grading, business, therapeutics, 030217 neurology & neurosurgery, Follow-Up Studies
Abstract: International audience; The aim of the present study was to evaluate the impact of first-line radiotherapy on low-grade gliomas (LGGs) growth kinetics. The mean tumor diameter (MTD) of 39 LGGs was retrospectively measured on serial magnetic resonance images before (n = 16) and after radiotherapy onset (n = 39). After radiotherapy, a decrease of the MTD was observed in 37 patients. Median duration of the MTD decrease was 1.9 years (range 0-8.1 years). According to RANO criteria, the rates of partial and minor responses were 15 and 28 % at the first evaluation after radiotherapy and 36 and 34 % at the time of maximal MTD decrease. The presence of a 1p19q codeletion and the absence of p53 expression were associated with longer durations of MTD decrease (5.3 vs 1 years, p = 0.02 and 2.4 vs 1.8 years, p = 0.05, respectively) while no association was observed between IDH1-R132H expression and duration of MTD decrease. In most patients, MTD decrease after radiotherapy occurred in two phases: an initial phase of rapid MTD decrease followed by a second phase of slower MTD decrease. Patients with a high rate of MTD decrease during the initial phase (>7 mm/year) had both a shorter duration of response (1.9 vs 5.3 years, p = 0.003) and a shorter overall survival (5.5 vs 11.6 years, p = 0.0004). LGGs commonly display a prolonged and ongoing volume decrease after radiotherapy. However, patients who respond rapidly should be carefully monitored because they are at a higher risk of rapid progression.
Published: 2013
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11. Langerin+ dendritic cells are responsible for LPS-induced reactivation of allergen-specific Th2 responses in postasthmatic mice

Author: Julie Cazareth, A Ortiz-Stern, Valérie Julia, David Dombrowicz, Akira Kanda, Anne Lazzari, Nicolas Glaichenhaus, Cyrille Mionnet, Sébastien Fleury, Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Immunologie des maladies infectieuses allergiques et autoimmunes, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Université Lille Nord de France (COMUE), Institut de pharmacologie moléculaire et cellulaire (IPMC), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Lipopolysaccharides, Lipopolysaccharide, MESH: Asthma, Receptors, Antigen, T-Cell, alpha-beta, [SDV]Life Sciences [q-bio], Lymphocyte Activation, medicine.disease_cause, Mice, chemistry.chemical_compound, 0302 clinical medicine, Allergen, Cell Movement, Immunology and Allergy, MESH: Animals, Receptor, Lung, MESH: Cell Movement, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, Mice, Inbred BALB C, 0303 health sciences, MESH: Receptors, Antigen, T-Cell, alpha-beta, biology, integumentary system, MESH: Dendritic Cells, respiratory system, 3. Good health, medicine.anatomical_structure, Antigens, Surface, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Cells, Cultured, MESH: Allergens, Langerin, Ovalbumin, MESH: Mice, Transgenic, MESH: Antigens, Surface, T cell, Immunology, MESH: Mice, Inbred BALB C, Mice, Transgenic, 03 medical and health sciences, Th2 Cells, Antigen, MESH: Eosinophils, MESH: Th2 Cells, MESH: Mannose-Binding Lectins, medicine, Animals, Humans, Lectins, C-Type, MESH: Lung, MESH: Lymphocyte Activation, MESH: Mice, 030304 developmental biology, MESH: Ovalbumin, MESH: Humans, Dendritic Cells, Allergens, Eosinophil, Asthma, respiratory tract diseases, Eosinophils, Mannose-Binding Lectins, chemistry, biology.protein, MESH: Lipopolysaccharides, MESH: Lectins, C-Type, 030215 immunology
Abstract: International audience; Allergic asthma is a T cell-dependent inflammatory lung disease that results from complex interactions between genetic predisposition and environmental factors, including exposure to lipopolysaccharide (LPS). In this study, we have shown that airway LPS exposure was sufficient to induce airway hyperreactivity (AHR) and eosinophil recruitment in mice that had previously experienced an acute episode of allergic asthma. LPS-induced disease reactivation depended on the activation of allergen-specific CD4(+) T cells by a subset of lung langerin(+) dendritic cells (DCs) that retained the allergen. Upon LPS exposure, migration of langerin(+) DCs from lungs to draining lymph nodes increased and LPS-exposed langerin(+) DCs instructed CD4(+) T cells toward a T helper (Th) 2 response. Selective depletion of langerin(+) DCs prevented LPS-induced eosinophil recruitment and T-cell activation, further demonstrating a critical role for langerin(+) DCs in disease reactivation. This finding provides a possible explanation for the subclinical worsening of asthmatics following exposure to low-dose LPS.
Published: 2011
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12. Cell biology and immunology of Leishmania

Author: Evelyne, Mougneau, Franck, Bihl, Nicolas, Glaichenhaus, Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de pharmacologie moléculaire et cellulaire (IPMC), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: MESH: Cell Differentiation, MESH: Cytokines, MESH: Leishmaniasis, MESH: Humans, T-Lymphocytes, Immunity, Cell Differentiation, adaptive immunity, protozoa, MESH: Leishmania major, MESH: T-Lymphocytes, vaccine, parasitic diseases, Animals, Cytokines, Humans, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Animals, MESH: Immune Evasion, Leishmaniasis, innate immunity, MESH: Immunity, Leishmania major, immune evasion
Abstract: International audience; More than 20 years ago, immunologists discovered that resistance and susceptibility to experimental infection with the intracellular protozoan Leishmania major was associated with the development of T-helper 1 (Th1)- and Th2-dominated immune responses, respectively. This infectious disease model was later used to identify and assess the role of key factors, such as interleukin-12 (IL-12) and IL-4, in Th1 and Th2 maturation. While infection by Leishmania remains a popular model for immunologists who wish to assess the role of their favorite molecule in T-cell differentiation, other investigators have tried to better understand how Leishmania interact with its insect and mammalian hosts. In this review, we discuss some of these new data with an emphasis on the early events that shape the immune response to Leishmania and on the immune evasion mechanisms that allow this parasite to avoid the development of sterilizing immunity and to secure its transmission to a new host.
Published: 2011
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13. CX3CR1 is required for airway inflammation by promoting T helper cell survival and maintenance in inflamed lung

Author: Nicolas Glaichenhaus, Robert L. Coffman, David Lair, Edith M. Hessel, Vanessa Buatois, Cyrille Mionnet, Valerie Milcent, Akira Kanda, Yannick Lacoeuille, Marie Langelot, Valérie Julia, David Dombrowicz, Antoine Magnan, Sébastien Fleury, Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 (RNMCD), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Réseau International des Instituts Pasteur (RIIP), Unité de recherche de l'institut du thorax (ITX-lab), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Dynavax Technologies, Dynavax, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Université Nice Sophia Antipolis (... - 2019) (UNS), unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: MESH: Signal Transduction, [SDV]Life Sciences [q-bio], Protozoan Proteins, MESH: Lymph Nodes, Apoptosis, Receptors, Interleukin-8A, Mice, Interleukin 21, 0302 clinical medicine, Cytotoxic T cell, MESH: Animals, MESH: Protozoan Proteins, Lung, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, General Medicine, T helper cell, MESH: Receptors, Interleukin-8A, respiratory system, 3. Good health, Phenotype, medicine.anatomical_structure, MESH: Cell Survival, Interleukin 13, [SDV.IMM]Life Sciences [q-bio]/Immunology, Receptors, Chemokine, Bronchial Hyperreactivity, medicine.symptom, Signal Transduction, MESH: Pneumonia, MESH: Mice, Transgenic, MESH: Hypersensitivity, Cell Survival, CX3C Chemokine Receptor 1, Antigens, Protozoan, Mice, Transgenic, Inflammation, Biology, MESH: Phenotype, General Biochemistry, Genetics and Molecular Biology, CCL5, 03 medical and health sciences, Th2 Cells, Antigen, MESH: Th2 Cells, MESH: Cell Proliferation, Hypersensitivity, medicine, Animals, MESH: Lung, MESH: Mice, Cell Proliferation, 030304 developmental biology, MESH: Apoptosis, MESH: Bronchial Hyperreactivity, Pneumonia, respiratory tract diseases, Immunology, Lymph Nodes, MESH: Receptors, Chemokine, MESH: Antigens, Protozoan, 030215 immunology
Abstract: International audience; Allergic asthma is a T helper type 2 (T(H)2)-dominated disease of the lung. In people with asthma, a fraction of CD4(+) T cells express the CX3CL1 receptor, CX3CR1, and CX3CL1 expression is increased in airway smooth muscle, lung endothelium and epithelium upon allergen challenge. Here we found that untreated CX3CR1-deficient mice or wild-type (WT) mice treated with CX3CR1-blocking reagents show reduced lung disease upon allergen sensitization and challenge. Transfer of WT CD4(+) T cells into CX3CR1-deficient mice restored the cardinal features of asthma, and CX3CR1-blocking reagents prevented airway inflammation in CX3CR1-deficient recipients injected with WT T(H)2 cells. We found that CX3CR1 signaling promoted T(H)2 survival in the inflamed lungs, and injection of B cell leukemia/lymphoma-2 protein (BCl-2)-transduced CX3CR1-deficient T(H)2 cells into CX3CR1-deficient mice restored asthma. CX3CR1-induced survival was also observed for T(H)1 cells upon airway inflammation but not under homeostatic conditions or upon peripheral inflammation. Therefore, CX3CR1 and CX3CL1 may represent attractive therapeutic targets in asthma.
Published: 2010
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14. The oral administration of bacterial extracts prevents asthma via the recruitment of regulatory T cells to the airways

Author: Valérie Julia, Tim Sparwasser, Anne Lazzari, C Chiavaroli, Akira Kanda, Nicolas Glaichenhaus, Severine Navarro, G Cossalter, David Dombrowicz, Julie Cazareth, Sébastien Fleury, Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Thoiry, Inconnu, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Université Lille Nord de France (COMUE), Institut de pharmacologie moléculaire et cellulaire (IPMC), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Institute of Infection Immunology (TWINCORE), Center for Experimental and Clinical Infection Research, and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)
Subjects: CD4-Positive T-Lymphocytes, Adoptive cell transfer, MESH: Interleukin-10, MESH: Asthma, Respiratory System, Administration, Oral, T-Lymphocytes, Regulatory, Mice, 0302 clinical medicine, Oral administration, MESH: Cell Extracts, Immunology and Allergy, MESH: Animals, MESH: Respiratory System, MESH: Cytokines, 0303 health sciences, Mice, Inbred BALB C, Respiratory tract infections, MESH: CD4-Positive T-Lymphocytes, FOXP3, Forkhead Transcription Factors, Adoptive Transfer, 3. Good health, Interleukin-10, MESH: Administration, Oral, [SDV.IMM]Life Sciences [q-bio]/Immunology, Cytokines, MESH: Myeloid Differentiation Factor 88, Oral treatment, Immunology, MESH: Mice, Inbred BALB C, 03 medical and health sciences, MESH: Forkhead Transcription Factors, medicine, Animals, MESH: Mice, Gene, 030304 developmental biology, Asthma, Bacteria, business.industry, MESH: T-Lymphocytes, Regulatory, medicine.disease, MESH: Adoptive Transfer, MESH: Bacteria, Myeloid Differentiation Factor 88, Airway, business, 030215 immunology
Abstract: International audience; The prevalence of asthma has steadily increased during the last decade, probably as the result of changes in the environment, including reduced microbial exposure during infancy. Accordingly, experimental studies have shown that deliberate infections with live pathogens prevent the development of allergic airway diseases in mice. Bacterial extracts are currently used in children suffering from repeated upper respiratory tract infections. In the present study, we have investigated whether bacterial extracts, commercially available as Broncho-Vaxom (BV), could prevent allergic airway disease in mice. Oral treatment with BV suppressed airway inflammation through interleukin-10 (IL-10)-dependent and MyD88 (myeloid differentiation primary response gene (88))-dependent mechanisms and induced the conversion of FoxP3 (forkhead box P3)(-) T cells into FoxP3(+) regulatory T cells. Furthermore, CD4(+) T cells purified from the trachea of BV-treated mice conferred protection against airway inflammation when adoptively transferred into sensitized mice. Therefore, treatment with BV could possibly be a safe and efficient strategy to prevent the development of allergic diseases in children.
Published: 2010
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15. Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development

Author: Eric Mosconi, Akira Kanda, Barbara Seitz-Polski, Sébastien Fleury, Akila Rekima, Nicolas Glaichenhaus, Valérie Verhasselt, Renato C. Monteiro, Valérie Julia, David Dombrowicz, Emilie Tissandie, Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Schistosomiase, paludisme et inflammation, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, Université Lille Nord de France (COMUE), Réseau International des Instituts Pasteur (RIIP), Immunopathologie rénale, récepteurs et inflammation, and Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Male, MESH: Asthma, MESH: Interleukin-2 Receptor alpha Subunit, Administration, Oral, Antigen-Antibody Complex, Receptors, Fc, T-Lymphocytes, Regulatory, MESH: Mice, Knockout, MESH: Animals, Newborn, MESH: Histocompatibility Antigens Class I, Mice, 0302 clinical medicine, MESH: Pregnancy, Pregnancy, Immunology and Allergy, MESH: Animals, IL-2 receptor, MESH: Maternal Exposure, Mice, Knockout, MESH: Immunoglobulin G, Mice, Inbred BALB C, 0303 health sciences, MESH: Milk, Human, FOXP3, Forkhead Transcription Factors, MESH: Antigen-Antibody Complex, 3. Good health, Tolerance induction, Breast Feeding, Maternal Exposure, MESH: Breast Feeding, MESH: Administration, Oral, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, MESH: Receptors, Fc, MESH: Allergens, MESH: Immune Tolerance, Ovalbumin, Immunology, MESH: Mice, Inbred BALB C, Breast milk, 03 medical and health sciences, Immune system, Neonatal Fc receptor, Antigen, MESH: Mice, Inbred C57BL, MESH: Forkhead Transcription Factors, Immune Tolerance, medicine, Animals, MESH: Mice, 030304 developmental biology, Asthma, MESH: Ovalbumin, Milk, Human, business.industry, MESH: T-Lymphocytes, Regulatory, Histocompatibility Antigens Class I, Interleukin-2 Receptor alpha Subunit, Allergens, medicine.disease, MESH: Male, Mice, Inbred C57BL, Animals, Newborn, Immunoglobulin G, MESH: Immunity, Maternally-Acquired, business, Immunity, Maternally-Acquired, MESH: Female, 030215 immunology
Abstract: International audience; Allergic asthma is a chronic lung disease resulting from an inappropriate T helper (Th)-2 response to environmental antigens. Early tolerance induction is an attractive approach for primary prevention of asthma. Here, we found that breastfeeding by antigen-sensitized mothers exposed to antigen aerosols during lactation induced a robust and long-lasting antigen-specific protection from asthma. Protection was more profound and persistent than the one induced by antigen-exposed non-sensitized mothers. Milk from antigen-exposed sensitized mothers contained antigen-immunoglobulin (Ig) G immune complexes that were transferred to the newborn through the neonatal Fc receptor resulting in the induction of antigen-specific FoxP3(+) CD25(+) regulatory T cells. The induction of oral tolerance by milk immune complexes did not require the presence of transforming growth factor-beta in milk in contrast to tolerance induced by milk-borne free antigen. Furthermore, neither the presence of IgA in milk nor the expression of the inhibitory FcgammaRIIb in the newborn was required for tolerance induction. This study provides new insights on the mechanisms of tolerance induction in neonates and highlights that IgG immune complexes found in breast milk are potent inducers of oral tolerance. These observations may pave the way for the identification of key factors for primary prevention of immune-mediated diseases such as asthma.
Published: 2010
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16. Primed antigen-specific CD4+ T cells are required for NK cell activation in vivo upon Leishmania major infection

Author: Franck Bihl, Julien Pecheur, Gwénola Poupon, Julie Cazareth, Béatrice Breart, Valérie Julia, Veronique M. Braud, Nicolas Glaichenhaus, Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Immunologie des maladies infectieuses allergiques et autoimmunes, and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: CD4-Positive T-Lymphocytes, Male, MESH: Interleukin-12, Time Factors, MESH: Flow Cytometry, Mice, SCID, MESH: Amino Acid Sequence, Lymphocyte Activation, MESH: Mice, Knockout, Mice, Interleukin 21, 0302 clinical medicine, MESH: Leishmania major, NK-92, Immunology and Allergy, MESH: Animals, MESH: Mice, SCID, Cells, Cultured, Leishmania major, Mice, Knockout, Mice, Inbred BALB C, 0303 health sciences, MESH: Dendritic Cells, Janus kinase 3, Innate lymphoid cell, MESH: CD4-Positive T-Lymphocytes, Flow Cytometry, Natural killer T cell, Interleukin-12, Cell biology, Killer Cells, Natural, Interleukin 12, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, MESH: Antigens, MESH: Cells, Cultured, MESH: Killer Cells, Natural, MESH: Interferon-gamma, Molecular Sequence Data, Immunology, MESH: Mice, Inbred BALB C, Leishmaniasis, Cutaneous, Biology, Interferon-gamma, 03 medical and health sciences, MESH: Mice, Inbred C57BL, Animals, Amino Acid Sequence, Antigens, Antigen-presenting cell, MESH: Lymphocyte Activation, MESH: Mice, 030304 developmental biology, MESH: Molecular Sequence Data, MESH: Time Factors, MESH: Interleukin-2, Dendritic Cells, MESH: Leishmaniasis, Cutaneous, MESH: Male, Mice, Inbred C57BL, Myeloid-derived Suppressor Cell, Interleukin-2, MESH: Female, 030215 immunology
Abstract: The ability of NK cells to rapidly produce IFN-γ is an important innate mechanism of resistance to many pathogens including Leishmania major. Molecular and cellular components involved in NK cell activation in vivo are still poorly defined, although a central role for dendritic cells has been described. In this study, we demonstrate that Ag-specific CD4+ T cells are required to initiate NK cell activation early on in draining lymph nodes of L. major-infected mice. We show that early IFN-γ secretion by NK cells is controlled by IL-2 and IL-12 and is dependent on CD40/CD40L interaction. These findings suggest that newly primed Ag-specific CD4+ T cells could directly activate NK cells through the secretion of IL-2 but also indirectly through the regulation of IL-12 secretion by dendritic cells. Our results reveal an unappreciated role for Ag-specific CD4+ T cells in the initiation of NK cell activation in vivo upon L. major infection and demonstrate bidirectional regulations between innate and adaptive immunity.
Published: 2010
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17. Human adenosine deaminase 2 induces differentiation of monocytes into macrophages and stimulates proliferation of T helper cells and macrophages

Author: Nicolas Glaichenhaus, Andrey V. Zavialov, Eduard Gracia, Anton V. Zavialov, Grégoire Lauvau, Rafael Franco, Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratory of Immune Regulation, Singapore Immunology Network (SIgN), Centro de Investigacion Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Instituto de Salud Carlos III [Madrid] (ISC), Department of Molecular Biology, and Swedish University of Agricultural Sciences (SLU)
Subjects: Adenosine Deaminase, Cell, MESH: Cell Cycle, MESH: Calcium-Binding Proteins, Adaptive Immunity, MESH: Receptor, Notch1, Monocytes, MESH: Receptor, Notch2, 0302 clinical medicine, MESH: Basic Helix-Loop-Helix Transcription Factors, Immunology and Allergy, MESH: Cell Movement, chemistry.chemical_classification, 0303 health sciences, Cell Differentiation, T-Lymphocytes, Helper-Inducer, Acquired immune system, MESH: Gene Expression Regulation, 3. Good health, Cell biology, medicine.anatomical_structure, MESH: Endometrium, 030220 oncology & carcinogenesis, Intercellular Signaling Peptides and Proteins, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Membrane Proteins, medicine.symptom, MESH: Neovascularization, Physiologic, medicine.drug, Immunology, Inflammation, Biology, Cell Line, MESH: Cell Adhesion, 03 medical and health sciences, Immune system, MESH: Cell Cycle Proteins, MESH: Homeodomain Proteins, MESH: Cell Communication, medicine, Humans, MESH: Intercellular Signaling Peptides and Proteins, Cell Proliferation, 030304 developmental biology, Innate immune system, MESH: Humans, Macrophages, Cell Biology, Adenosine, Immunity, Innate, Enzyme, chemistry, MESH: Oligonucleotide Array Sequence Analysis, MESH: Stromal Cells, MESH: Receptors, Notch, Macrophage proliferation, MESH: Female
Abstract: Discovery of the growth factor activity of ADA2. ADAs play a pivotal role in regulating the level of adenosine, a signaling molecule controlling a variety of cellular responses by binding to and activating four ADRs. Two enzymes, ADA1 and ADA2, are known to possess ADA activity in humans. Although the structure of ADA1 and its role in lymphocytic activation have been known for a long time, the structure and function of ADA2, a member of ADGF, remain enigmatic. Here, we found that ADA2 is secreted by monocytes undergoing differentiation into macrophages or DCs and that it binds to the cell surface via proteoglycans and ADRs. We demonstrate that ADA1 and ADA2 increase the rate of proliferation of monocyte-activated CD4+ T cells independently of their catalytic activity. We also show that ADA2 induces T cell-dependent differentiation of monocytes into macrophages and stimulates macrophage proliferation. Our discovery of the growth factor-like activity of ADA2 explains clinical observations and suggests that this enzyme could be used as a drug candidate to modulate the immune responses during inflammation and cancer.
Published: 2010
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18. Visualizing early splenic memory CD8+ T cells reactivation against intracellular bacteria in the mouse

Author: Grégoire Lauvau, Marc Bajénoff, Frédéric Brau, Emilie Narni-Mancinelli, Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Immunologie des maladies infectieuses allergiques et autoimmunes, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM : AVENIR, ANR-08-MIEN-0008,EMICIF,Caractérisation in vivo des lymphocytes T CD8 effecteurs et mémoires antimicrobiens au cours de réponses immunitaires primaires et secondaires: mécanismes d'induction, fonctions protectrices, et interactions avec les cellules de l'immunité innée(2008), ANR-08-JCJC-0134,RCS,Rôle des cellules stromales dans l'organisation et la fonction des organes lymphoides secondaires(2008), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Université Nice Sophia Antipolis (... - 2019) (UNS), Centre d'Immunologie de Marseille - Luminy ( CIML ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Aix Marseille Université ( AMU ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de pharmacologie moléculaire et cellulaire ( IPMC ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), ANR-08-MIEN-0008,EMICIF,Caractérisation in vivo des lymphocytes T CD8 effecteurs et mémoires antimicrobiens au cours de réponses immunitaires primaires et secondaires: mécanismes d'induction, fonctions protectrices, et interactions avec les cellules de l'immunité innée ( 2008 ), and ANR-08-JCJC-0134,RCS,Rôle des cellules stromales dans l'organisation et la fonction des organes lymphoides secondaires ( 2008 )
Subjects: MESH: Spleen, lcsh:Medicine, MESH: Flow Cytometry, CD8-Positive T-Lymphocytes, MESH: Listeria monocytogenes, MESH: Receptors, CCR7, Interleukin 21, Mice, 0302 clinical medicine, MESH : Chemokine CCL3, MESH : Receptors, CCR7, Cytotoxic T cell, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH: L-Selectin, Listeriosis, MESH: Animals, IL-2 receptor, L-Selectin, lcsh:Science, Chemokine CCL3, 0303 health sciences, Mice, Inbred BALB C, Multidisciplinary, ZAP70, MESH : CD8-Positive T-Lymphocytes, Acquired immune system, Flow Cytometry, MESH: CD8-Positive T-Lymphocytes, Cell biology, Infectious Diseases, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH : L-Selectin, Research Article, MESH : Spleen, Receptors, CCR7, MESH : Flow Cytometry, MESH: Interferon-gamma, Immunology, MESH: Mice, Inbred BALB C, MESH : Mice, Inbred C57BL, Biology, 03 medical and health sciences, Interferon-gamma, Immune system, MESH: Mice, Inbred C57BL, Immunology/Immunity to Infections, MESH : Mice, Animals, MESH: Chemokine CCL3, MESH: Mice, MESH : Mice, Inbred BALB C, MESH : Interferon-gamma, 030304 developmental biology, Interleukin 3, lcsh:R, Listeria monocytogenes, Mice, Inbred C57BL, MESH: Listeriosis, lcsh:Q, MESH : Animals, MESH : Listeria monocytogenes, MESH : Listeriosis, CD8, Spleen, 030215 immunology
Abstract: International audience; Memory CD8(+) T cells represent an important effector arm of the immune response in maintaining long-lived protective immunity against viruses and some intracellular bacteria such as Listeria monocytogenes (L.m). Memory CD8(+) T cells are endowed with enhanced antimicrobial effector functions that perfectly tail them to rapidly eradicate invading pathogens. It is largely accepted that these functions are sufficient to explain how memory CD8(+) T cells can mediate rapid protection. However, it is important to point out that such improved functional features would be useless if memory cells were unable to rapidly find the pathogen loaded/infected cells within the infected organ. Growing evidences suggest that the anatomy of secondary lymphoid organs (SLOs) fosters the cellular interactions required to initiate naive adaptive immune responses. However, very little is known on how the SLOs structures regulate memory immune responses. Using Listeria monocytogenes (L.m) as a murine infection model and imaging techniques, we have investigated if and how the architecture of the spleen plays a role in the reactivation of memory CD8(+) T cells and the subsequent control of L.m growth. We observed that in the mouse, memory CD8(+) T cells start to control L.m burden 6 hours after the challenge infection. At this very early time point, L.m-specific and non-specific memory CD8(+) T cells localize in the splenic red pulp and form clusters around L.m infected cells while naïve CD8(+) T cells remain in the white pulp. Within these clusters that only last few hours, memory CD8(+) T produce inflammatory cytokines such as IFN-gamma and CCL3 nearby infected myeloid cells known to be crucial for L.m killing. Altogether, we describe how memory CD8(+) T cells trafficking properties and the splenic micro-anatomy conjugate to create a spatio-temporal window during which memory CD8(+) T cells provide a local response by secreting effector molecules around infected cells.
Published: 2010
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19. Direct Visualization of Peptide/MHC Complexes at the Surface and in the Intracellular Compartments of Cells Infected In Vivo by Leishmania major

Author: Christoph Lippuner, Evelyne Mougneau, Eric Muraille, Julie Cazareth, Toni Aebischer, Johan Hoebeke, Nicolas Glaichenhaus, Pierre Gounon, Ana A. Davalos-Misslitz, Sylviane Muller, Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Institut de pharmacologie moléculaire et cellulaire (IPMC), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Immunologie et chimie thérapeutiques (ICT), Cancéropôle du Grand Est-Centre National de la Recherche Scientifique (CNRS), Vetsuisse Faculty, Vetsuisse Faculty University Zürich, Department of Nephrology (MHH), Hannover Medical School [Hannover] (MHH), Parasitology laboratory, and Robert Koch Institute [Berlin] (RKI)
Subjects: Fluorescent Antibody Technique, Virologie générale, Cell membrane, Mice, 0302 clinical medicine, Mice, Inbred NOD, Phagosomes, Immunologie, Leishmania major, Microbiology/Parasitology, Biology (General), Cells, Cultured, 0303 health sciences, Parasitologie, Mice, Inbred BALB C, Mice, Inbred C3H, biology, 3. Good health, Cell biology, Microbiology/Immunity to Infections, medicine.anatomical_structure, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Interleukin 12, Immunology/Antigen Processing and Recognition, [SDV.IMM]Life Sciences [q-bio]/Immunology, Biologie, Research Article, QH301-705.5, Immunology, Antigen presentation, Leishmaniasis, Cutaneous, Major histocompatibility complex, Microbiology, 03 medical and health sciences, Antigen, Virology, Immunology/Immunity to Infections, Genetics, medicine, Animals, Amino Acid Sequence, Antigen-presenting cell, Molecular Biology, 030304 developmental biology, CD40, Cell Membrane, Virologie médicale, Histocompatibility Antigens Class II, Infectious Diseases/Protozoal Infections, Biologie moléculaire, Intracellular Membranes, RC581-607, biology.organism_classification, Molecular biology, Peptide Fragments, Cell Compartmentation, Mice, Inbred C57BL, biology.protein, Parasitology, Immunologic diseases. Allergy, Microbiologie et protistologie [bacteriol.virolog.mycolog.], 030215 immunology
Abstract: Protozoa and bacteria infect various types of phagocytic cells including macrophages, monocytes, dendritic cells and eosinophils. However, it is not clear which of these cells process and present microbial antigens in vivo and in which cellular compartments parasite peptides are loaded onto Major Histocompatibility Complex molecules. To address these issues, we have infected susceptible BALB/c (H-2d) mice with a recombinant Leishmania major parasite expressing a fluorescent tracer. To directly visualize the antigen presenting cells that present parasite-derived peptides to CD4+ T cells, we have generated a monoclonal antibody that reacts to an antigenic peptide derived from the parasite LACK antigen bound to I-Ad Major Histocompatibility Complex class II molecule. Immunogold electron microscopic analysis of in vivo infected cells showed that intracellular I-Ad/LACK complexes were present in the membrane of amastigote-containing phagosomes in dendritic cells, eosinophils and macrophages/monocytes. In both dendritic cells and macrophages, these complexes were also present in smaller vesicles that did not contain amastigote. The presence of I-Ad/LACK complexes at the surface of dendritic cells, but neither on the plasma membrane of macrophages nor eosinophils was independently confirmed by flow cytometry and by incubating sorted phagocytes with highly sensitive LACK-specific hybridomas. Altogether, our results suggest that peptides derived from Leishmania proteins are loaded onto Major Histocompatibility Complex class II molecules in the phagosomes of infected phagocytes. Although these complexes are transported to the cell surface in dendritic cells, therefore allowing the stimulation of parasite-specific CD4+ T cells, this does not occur in other phagocytic cells. To our knowledge, this is the first study in which Major Histocompatibility Complex class II molecules bound to peptides derived from a parasite protein have been visualized within and at the surface of cells that were infected in vivo. © 2010 Muraille et al., SCOPUS: ar.j, info:eu-repo/semantics/published
Published: 2010
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20. CX(3)CR1(+) CD115(+) CD135(+) common macrophage/DC precursors and the role of CX(3)CR1 in their response to inflammation

Author: Cédric Auffray, Ana Cumano, Grégoire Lauvau, Laura Campisi, Frederic Geissmann, Darin K. Fogg, Thierry Jo Molina, David A. Hume, Céline Trouillet, Brigitte Senechal, Julia Leemput, Karine Bigot, Emilie Narni-Mancinelli, Marc Abitbol, Noah Saederup, Israel F. Charo, Système des phagocytes mononucléés et immunopathologie, Université Paris Descartes - Paris 5 (UPD5), Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre for Cellular and Molecular Biology of Inflammation, King‘s College London, Gladstone Institute of Cardiovascular Disease, University of California [San Francisco] (UC San Francisco), University of California (UC)-University of California (UC), Centre d'étude et de recherche thérapeutiques en ophtalmologie, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Développement des Lymphocytes, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by an European Young Investigator (EURYI) award to F. Geissmann, grants from Institut National de la Sant é et de la Recherche M é dicale (Avenir) and Human Frontier Science Program (CDA) to G. Lauvau, grants from the Agence Nationale de la Recherche (ANR IRAP2005) to F. Geissmann and G. Lauvau, and grants from the Fondation pour la Recherche Medicale to F. Geissmann (Equipe FRM 2006) and to G. Lauvau (FRM equipments fund)., Université Nice Sophia Antipolis (... - 2019) (UNS), University of California [San Francisco] (UCSF), University of California-University of California, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Vougny, Marie-Christine, Université Paris Descartes - Paris 5 ( UPD5 ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), University of California [San Francisco] ( UCSF ), and Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale ( INSERM )
Subjects: MESH: Inflammation, Monocytes/cytology, MESH: Spleen, MESH: Monocytes, MESH: Listeria monocytogenes, Monocytes, Mice, 0302 clinical medicine, Cell Movement, Spleen/cytology, MESH : Cell Proliferation, MESH : Cell Movement, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, Macrophage, MESH: Animals, MESH: Cell Movement, MESH : Macrophages, MESH : Cell Survival, 0303 health sciences, MESH: Bone Marrow Cells, Stem Cells, hemic and immune systems, Dendritic Cells/enzymology, Receptors, Chemokine/metabolism, 3. Good health, Cell biology, MESH : Phenotype, MESH: Cell Survival, Tumor necrosis factor alpha, Receptors, Chemokine, MESH : Cell Differentiation, MESH: fms-Like Tyrosine Kinase 3, MESH: Receptor, Macrophage Colony-Stimulating Factor, [SDV.IMM] Life Sciences [q-bio]/Immunology, Immunology, CX3C Chemokine Receptor 1, Bone Marrow Cells/cytology, Tumor Necrosis Factor-alpha/biosynthesis, Spleen, Dendritic Cells/cytology, MESH: Phenotype, Article, 03 medical and health sciences, Receptor, Macrophage Colony-Stimulating Factor/metabolism, MESH : Inflammation, fms-Like Tyrosine Kinase 3/metabolism, Tumor Necrosis Factor-alpha, MESH : Listeria Infections, Macrophages, Monocytes/immunology, Dendritic cell, Dendritic Cells, Listeria monocytogenes, fms-Like Tyrosine Kinase 3, Dendritic Cells/immunology, MESH : Dendritic Cells, MESH: Tumor Necrosis Factor-alpha, Reactive Oxygen Species, MESH : Bone Marrow Cells, Myeloid, MESH : Reactive Oxygen Species, Nitric Oxide Synthase Type II, Macrophages/immunology, Inflammation/enzymology, Immunology and Allergy, Listeriosis, MESH : Tumor Necrosis Factor-alpha, MESH: Dendritic Cells, MESH: Reactive Oxygen Species, Cell Differentiation, Inflammation/immunology, Listeriosis/immunology, medicine.anatomical_structure, Phenotype, MESH : Stem Cells, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Nitric Oxide Synthase Type II, medicine.symptom, Stem cell, MESH: Listeria Infections, Nitric Oxide Synthase Type II/metabolism, MESH : Spleen, MESH: Cell Differentiation, MESH : Receptors, Chemokine, MESH : fms-Like Tyrosine Kinase 3, Cell Survival, Macrophages/enzymology, Inflammation, Bone Marrow Cells, Receptor, Macrophage Colony-Stimulating Factor, MESH: Stem Cells, Biology, MESH : Receptor, Macrophage Colony-Stimulating Factor, Reactive Oxygen Species/metabolism, MESH: Cell Proliferation, MESH : Mice, medicine, Animals, MESH: Mice, 030304 developmental biology, Cell Proliferation, MESH: Macrophages, Macrophages/cytology, MESH : Nitric Oxide Synthase Type II, Receptors, Chemokine/deficiency, MESH : Monocytes, Fms-Like Tyrosine Kinase 3, MESH : Animals, MESH : Listeria monocytogenes, MESH: Receptors, Chemokine, Bone Marrow Cells/immunology, 030215 immunology
Abstract: International audience; CX(3)CR1 expression is associated with the commitment of CSF-1R(+) myeloid precursors to the macrophage/dendritic cell (DC) lineage. However, the relationship of the CSF-1R(+) CX(3)CR1(+) macrophage/DC precursor (MDP) with other DC precursors and the role of CX(3)CR1 in macrophage and DC development remain unclear. We show that MDPs give rise to conventional DCs (cDCs), plasmacytoid DCs (PDCs), and monocytes, including Gr1(+) inflammatory monocytes that differentiate into TipDCs during infection. CX(3)CR1 deficiency selectively impairs the recruitment of blood Gr1(+) monocytes in the spleen after transfer and during acute Listeria monocytogenes infection but does not affect the development of monocytes, cDCs, and PDCs.
Published: 2009
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21. A dynamic map of antigen recognition by CD4 T cells at the site of Leishmania major infection

Author: Nicolas Glaichenhaus, Béatrice Breart, Gerald F. Späth, Toni Aebischer, Philippe Bousso, Christoph Lippuner, Pascale Pescher, Orchidée Filipe-Santos, Dynamiques des Réponses Immunes, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Equipe Avenir, Institut National de la Santé et de la Recherche Médicale (INSERM), Virulence Parasitaire, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Inserm Avenir Group, Joint Appointment Institute of Parasitology and Section Herd Health Management, Vetsuisse Faculty University Zürich, Institute of Immunology and Infection Research, University of Edinburgh, Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Université Nice Sophia Antipolis (1965 - 2019) (UNS)
Subjects: CD4-Positive T-Lymphocytes, Cancer Research, MICROBIO, CD4 antigen, T cell, Antigens, Protozoan, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Mice, 0302 clinical medicine, Immune system, Antigen, Dermis, In vivo, Virology, Immunology and Microbiology(all), medicine, Animals, Leishmania major, Molecular Biology, Leishmaniasis, 030304 developmental biology, Mice, Knockout, 0303 health sciences, Mice, Inbred BALB C, Microscopy, Confocal, biology, Staining and Labeling, Intracellular parasite, Dendritic Cells, biology.organism_classification, Cell biology, Mice, Inbred C57BL, medicine.anatomical_structure, chemistry, Microscopy, Fluorescence, CELLIMMUNO, Host-Pathogen Interactions, [SDV.IMM]Life Sciences [q-bio]/Immunology, Parasitology, 030215 immunology
Abstract: International audience; CD4 T helper cells play a central role in the control of infection by intracellular parasites. How efficiently pathogen-specific CD4 T cells detect infected cells in vivo is unclear. Here, we employed intravital two-photon imaging to examine the behavior of pathogen-specific CD4 T cells at the site of Leishmania major infection. While activated CD4 T cells enter the inflamed tissue irrespective of their antigen specificity, pathogen-specific T cells preferentially decelerated and accumulated in infected regions of the dermis. Antigen recognition by CD4 T cells was heterogeneous, involving both stable and dynamic contacts with infected phagocytes. However, not all infected cells induced arrest or deceleration of pathogen-specific T cells, and dense clusters of infected cells were poorly accessible to migrating T cells. Thus, disparities in the dynamics of T cell contacts with infected cells and local variation in T cell access to infected cells are important elements of the host-pathogen interplay.
Published: 2008
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22. [A new vision for autoimmunity]

Author: Glaichenhaus, Nicolas, Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: MESH: Humans, MESH: Th1 Cells, MESH: Interleukin-17, MESH: T-Lymphocytes, Regulatory, Interleukin-17, MESH: Autoimmunity, Humans, [SDV.IMM]Life Sciences [q-bio]/Immunology, Autoimmunity, Th1 Cells, T-Lymphocytes, Regulatory, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2008

23. CD4+CD25+ Tregs control the TRAIL-dependent cytotoxicity of tumor-infiltrating DCs in rodent models of colon cancer

Author: Bruno Chauffert, Grégoire Lauvau, Eric Solary, Hideo Yagita, Laurence Zitvogel, François Martin, Stephan Roux, Fanny Chalmin, Pierre Emmanuel Puig, Grégoire Mignot, Sylvain Ladoire, François Ghiringhelli, Lionel Apetoh, Role des Cellules Dendritiques Dans la Regulation des Effecteurs de l'Immunite Antitumorale, Université Paris-Sud - Paris 11 ( UP11 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc ( CRLCC - CGFL ), Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Department of Immunology, Juntendo University School of Medicine, We received grant support from Fondation pour la Recherche Médicale (to S. Roux and L. Apetoh), Association pour la Recherche contre le Cancer (to G. Mignot), and Institut National de la Santé et de la Recherche Médicale (to F. Ghiringhelli). This work was supported by special grants from the Ligue contre le Cancer de Bourgogne and the Association pour la Recherche contre le Cancer., Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Mignot, Grégoire, and Université Nice Sophia Antipolis (1965 - 2019) (UNS)
Subjects: CD4-Positive T-Lymphocytes, Male, MESH : Rats, Inbred Strains, T-Lymphocytes, Regulatory, MESH : TNF-Related Apoptosis-Inducing Ligand, TNF-Related Apoptosis-Inducing Ligand, MESH : T-Lymphocytes, Regulatory, Mice, 0302 clinical medicine, Neoplasms, Cytotoxic T cell, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH: Animals, MESH: Neoplasms, IL-2 receptor, Cytotoxicity, Cells, Cultured, 0303 health sciences, Mice, Inbred BALB C, MESH: Dendritic Cells, MESH : Rats, MESH: CD4-Positive T-Lymphocytes, MESH : Mice, Nude, hemic and immune systems, General Medicine, MESH: Rats, Inbred Strains, 3. Good health, MESH : Colonic Neoplasms, MESH : CD4-Positive T-Lymphocytes, Colonic Neoplasms, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: TNF-Related Apoptosis-Inducing Ligand, MESH: Cells, Cultured, Research Article, MESH: Cell Line, Tumor, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH: Rats, MESH : Male, MESH: Mice, Inbred BALB C, Mice, Nude, chemical and pharmacologic phenomena, Biology, 03 medical and health sciences, Immune system, Cell Line, Tumor, MESH : Cells, Cultured, MESH : Mice, MESH: Mice, Nude, Animals, MESH: Mice, MESH : Mice, Inbred BALB C, 030304 developmental biology, MESH: Colonic Neoplasms, Innate immune system, MESH : Cell Line, Tumor, MESH: T-Lymphocytes, Regulatory, TLR9, Rats, Inbred Strains, Dendritic Cells, MESH : Neoplasms, MESH : Disease Models, Animal, MESH: Male, Rats, TLR2, Disease Models, Animal, MESH : Dendritic Cells, Immunology, TLR4, MESH : Animals, MESH: Disease Models, Animal, 030215 immunology
Abstract: International audience; Tumors that progress do so via their ability to escape the antitumor immune response through several mechanisms, including developing ways to induce the differentiation and/or recruitment of CD4(+)CD25(+) Tregs. The Tregs, in turn, inhibit the cytotoxic function of T cells and NK cells, but whether they have an effect on the cytotoxic function of tumor-infiltrating DCs (TIDCs) has not been determined. Here we have shown, in 2 rodent models of colon cancer, that CD4(+)CD25(+) Tregs inhibit the ability of CD11b(+) TIDCs to mediate TNF-related apoptosis-inducing ligand-induced (TRAIL-induced) tumor cell death. In both models of cancer, combination treatment with Mycobacterium bovis Bacillus Calmette-Guérin (BCG), which activates the innate immune system via TLR2, TLR4, and TLR9, and cyclophosphamide (CTX), which depletes Tregs, eradicated the tumors. Further analysis revealed that the treatment led to a marked increase in the number of CD11b(+) TIDCs that killed the tumor cells via a TRAIL-dependent mechanism. Furthermore, acquisition of TRAIL expression by the CD11b(+) TIDCs was induced by BCG and dependent on signaling through TLR2, TLR4, and TLR9. In vivo transfer of Tregs abrogated the ability of BCG to induce CD11b(+) TIDCs to express TRAIL and thereby nullified the efficacy of the CTX-BCG treatment. Our data have therefore delineated what we believe to be a novel mechanism by which Tregs inhibit the antitumor immune response.
Published: 2008
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24. Fibroblastic reticular cells guide T lymphocyte entry into and migration within the splenic T cell zone

Author: Marc Bajénoff, Ronald N. Germain, Nicolas Glaichenhaus, Lymphocyte Biology Section ( NIAID ), National Institutes of Health, Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Lymphocyte Biology Section (NIAID), Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Pathology, MESH: Spleen, MESH: Mice, Mutant Strains, Lymphocyte, MESH: Chemotaxis, Leukocyte, MESH : Arterioles, MESH: T-Lymphocyte Subsets, MESH : Radiation Chimera, 0302 clinical medicine, Reticular cell, Immunology and Allergy, Cytotoxic T cell, MESH: Microscopy, Confocal, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH: Animals, MESH : Chemokine CCL21, 0303 health sciences, MESH : Adoptive Transfer, MESH : Chemotaxis, Leukocyte, Marginal zone, Cell biology, medicine.anatomical_structure, [SDV.IMM]Life Sciences [q-bio]/Immunology, Lymph, MESH: B-Lymphocyte Subsets, MESH : Spleen, medicine.medical_specialty, MESH: Radiation Chimera, T cell, Immunology, MESH : Mice, Inbred C57BL, Biology, 03 medical and health sciences, MESH: Arterioles, MESH: Mice, Inbred C57BL, MESH : Fibroblasts, MESH : Mice, medicine, MESH : Microscopy, Confocal, MESH: Mice, 030304 developmental biology, MESH: Chemokine CCL21, T lymphocyte, MESH : T-Lymphocyte Subsets, MESH : Mice, Mutant Strains, MESH: Adoptive Transfer, MESH: Fibroblasts, MESH : Stromal Cells, MESH : Animals, MESH: Stromal Cells, Periarteriolar lymphoid sheaths, 030215 immunology, MESH : B-Lymphocyte Subsets
Abstract: Although a great deal is known about T cell entry into lymph nodes, much less is understood about how T lymphocytes access the splenic white pulp (WP). We show in this study that, as recently described for lymph nodes, fibroblastic reticular cells (FRCs) form a network in the T cell zone (periarteriolar lymphoid sheath, PALS) of the WP on which T lymphocytes migrate. This network connects the PALS to the marginal zone (MZ), which is the initial site of lymphocyte entry from the blood. T cells do not enter the WP at random locations but instead traffic to that site using the FRC-rich MZ bridging channels (MZBCs). These data reveal that FRCs form a substrate for T cells in the spleen, guiding these lymphocytes from their site of entry in the MZ into the PALS, within which they continue to move on the same network.
Published: 2008

25. Breast milk-mediated transfer of an antigen induces tolerance and protection from allergic asthma

Author: Julie Cazareth, Valerie Milcent, Valérie Verhasselt, David Dombrowicz, Valérie Julia, Nicolas Glaichenhaus, Akira Kanda, Sébastien Fleury, Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Institut de pharmacologie moléculaire et cellulaire (IPMC), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Schistosomiase, paludisme et inflammation, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, Université de Lille, Fondation pour la Recherche Médicale, European Union (DC-THERA), US Juvenile Diabetes Research Foundation, Belgian Fond National de la Recherche Scientifique, and Université Nice Sophia Antipolis (1965 - 2019) (UNS)
Subjects: MESH: Immunoglobulins, MESH: Asthma, T-Lymphocytes, Regulatory, Airborne allergen, Mice, 0302 clinical medicine, Lactation, MESH: Animals, MESH: Maternal Exposure, 0303 health sciences, Mice, Inbred BALB C, biology, General Medicine, 3. Good health, Tolerance induction, medicine.anatomical_structure, Milk, Maternal Exposure, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, MESH: Antigens, Antibody, Bronchial Hyperreactivity, MESH: Pneumonia, MESH: Lactation, MESH: Immune Tolerance, Ovalbumin, MESH: Hypersensitivity, MESH: Mice, Inbred BALB C, Immunoglobulins, Breast milk, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Antigen, MESH: Mice, Inbred C57BL, medicine, Hypersensitivity, Immune Tolerance, Animals, Antigens, MESH: Mice, 030304 developmental biology, Asthma, MESH: Ovalbumin, business.industry, MESH: T-Lymphocytes, Regulatory, MESH: Bronchial Hyperreactivity, Pneumonia, Airway obstruction, medicine.disease, Mice, Inbred C57BL, MESH: Milk, Immunology, biology.protein, business, MESH: Female, 030215 immunology
Abstract: Allergic asthma is a chronic disease characterized by airway obstruction in response to allergen exposure. It results from an inappropriate T helper type 2 response to environmental airborne antigens and affects 300 million individuals. Its prevalence has increased markedly in recent decades, most probably as a result of changes in environmental factors. Exposure to environmental antigens during infancy is crucial to the development of asthma. Epidemiological studies on the relationship between breastfeeding and allergic diseases have reached conflicting results. Here, we have investigated whether the exposure of lactating mice to an airborne allergen affects asthma development in progeny. We found that airborne antigens were efficiently transferred from the mother to the neonate through milk and that tolerance induction did not require the transfer of immunoglobulins. Breastfeeding-induced tolerance relied on the presence of transforming growth factor (TGF)-beta during lactation, was mediated by regulatory CD4+ T lymphocytes and depended on TGF-beta signaling in T cells. In conclusion, breast milk-mediated transfer of an antigen to the neonate resulted in oral tolerance induction leading to antigen-specific protection from allergic airway disease. This study may pave the way for the design of new strategies to prevent the development of allergic diseases.
Published: 2008
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26. Imaging host-pathogen interactions

Author: Nicolas Glaichenhaus, Laura Campisi, Frédéric Brau, Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Institut de pharmacologie moléculaire et cellulaire (IPMC), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Diagnostic Imaging, 0303 health sciences, MESH: Humans, MESH: Diagnostic Imaging, Immunology, MESH: Host-Pathogen Interactions, Computational biology, Biology, 3. Good health, Cell biology, 03 medical and health sciences, 0302 clinical medicine, Sepsis, Host-Pathogen Interactions, Immunology and Allergy, Animals, Humans, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Animals, Host (network), Pathogen, MESH: Sepsis, 030304 developmental biology, 030215 immunology
Abstract: Imaging techniques have been revolutionized by advancements in both microscope instrumentation and data collection processing software. Immunologists and microbiologists now have access to a large panel of powerful technologies that are characterized by different spatial and time resolutions. In this review, we discuss recent studies in which emerging imaging technologies have been used to decipher the complexity of the interactions between pathogens and their mammalian hosts. By focusing on two very different pathogens, Plasmodium and Salmonella, we emphasize the critical role of imaging studies in the understanding of the host's immune system response to a pathogen.
Published: 2008
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27. Memory CD8+ T cells mediate antibacterial immunity via CCL3 activation of TNF/ROI+ phagocytes

Author: Laura Campisi, Delphine Bassand, Grégoire Lauvau, Pierre Gounon, Emilie Narni-Mancinelli, Nicolas Glaichenhaus, Julie Cazareth, Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Institut de pharmacologie moléculaire et cellulaire (IPMC), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), and INSERM (Avenir), Human Frontier Science Program (CDA), Agence Nationale de la Recherche (ANR-IRAP-2005), Fondation pour la Recherche Médicale (FRM)
Subjects: Cytotoxicity, Immunologic, Chemokine, Time Factors, Neutrophils, MESH: Neutrophils, CD8-Positive T-Lymphocytes, MESH: Listeria monocytogenes, MESH: Phagocytes, Mice, 0302 clinical medicine, MESH: Leishmania major, Immunology and Allergy, Cytotoxic T cell, Interferon gamma, MESH: Animals, Listeriosis, Chemokine CCL4, MESH: Bacterial Proteins, MESH: Immunity, Chemokine CCL3, Leishmania major, 0303 health sciences, Phagocytes, biology, Effector, MESH: Bystander Effect, MESH: Reactive Oxygen Species, MESH: Tumor Necrosis Factors, Articles, Macrophage Inflammatory Proteins, MESH: CD8-Positive T-Lymphocytes, MESH: Chemokines, CC, Chemokines, CC, Tumor Necrosis Factors, MESH: Immunologic Memory, MESH: Immunization, [SDV.IMM]Life Sciences [q-bio]/Immunology, Tumor necrosis factor alpha, Female, MESH: Listeria Infections, medicine.drug, MESH: Mutation, MESH: Interferon Type II, MESH: Macrophage Inflammatory Proteins, Secondary infection, Immunology, MESH: Models, Immunological, Article, Microbiology, 03 medical and health sciences, Interferon-gamma, Bacterial Proteins, medicine, Animals, MESH: Chemokine CCL3, MESH: Cytotoxicity, Immunologic, MESH: Chemokine CCL4, MESH: Mice, 030304 developmental biology, Innate immune system, Tumor Necrosis Factor-alpha, MESH: Time Factors, Immunity, Models, Immunological, Bystander Effect, Listeria monocytogenes, MESH: Tumor Necrosis Factor-alpha, Mutation, biology.protein, Immunization, Reactive Oxygen Species, MESH: Female, Immunologic Memory, CD8, 030215 immunology
Abstract: Cytolysis, interferon γ and tumor necrosis factor (TNF) α secretion are major effector mechanisms of memory CD8+ T cells that are believed to be required for immunological protection in vivo. By using mutants of the intracellular bacterium Listeria monocytogenes, we found that none of these effector activities is sufficient to protect against secondary infection with wild-type (WT) bacteria. We demonstrated that CCL3 derived from reactivated memory CD8+ T cells is required for efficient killing of WT bacteria. CCL3 induces a rapid TNF-α secretion by innate inflammatory mononuclear phagocytic cells (MPCs), which further promotes the production of radical oxygen intermediates (ROIs) by both MPCs and neutrophils. ROI generation is the final bactericidal mechanism involved in L. monocytogenes clearance. These results therefore uncover two levels of regulation of the antibacterial secondary protective response: (a) an antigen-dependent phase in which memory CD8+ T cells are reactivated and control the activation of the innate immune system, and (b) an antigen-independent phase in which the MPCs coordinate innate immunity and promote the bactericidal effector activities. In this context, CCL3-secreting memory CD8+ T cells are able to mediate “bystander” killing of an unrelated pathogen upon antigen-specific reactivation, a mechanism that may be important for the design of therapeutic vaccines.
Published: 2007
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28. Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior

Author: Darin K. Fogg, Frederic Geissmann, Grégoire Lauvau, Sabine Sarnacki, Cédric Auffray, Meriem Garfa, Ana Cumano, Olivier Join-Lambert, Samer Kayal, Gaelle Elain, Système des phagocytes mononucléés et immunopathologie, Université Paris Descartes - Paris 5 (UPD5), CHU Necker - Enfants Malades [AP-HP], Développement des Lymphocytes, Institut Pasteur [Paris] - Institut National de la Santé et de la Recherche Médicale (INSERM), Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis (UNS), Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: MESH: Inflammation, Cellular immunity, MESH: Mesenteric Arteries, MESH: Listeria monocytogenes, MESH: Monocytes, Monocytes, Mice, 0302 clinical medicine, Cell Movement, CX3CR1, MESH: Microscopy, Confocal, Listeriosis, MESH: Animals, MESH: Cell Movement, 0303 health sciences, education.field_of_study, Microscopy, Confocal, Multidisciplinary, Cell Differentiation, Dermis, MESH: Gene Expression Regulation, Lymphocyte Function-Associated Antigen-1, Extravasation, Mesenteric Arteries, medicine.anatomical_structure, [SDV.IMM]Life Sciences [q-bio]/Immunology, Receptors, Chemokine, MESH: Endothelium, Vascular, medicine.symptom, MESH: Listeria Infections, Blood vessel, MESH: Cell Differentiation, Endothelium, [SDV.IMM] Life Sciences [q-bio]/Immunology, Population, MESH: Lymphocyte Function-Associated Antigen-1, CX3C Chemokine Receptor 1, Inflammation, Biology, MESH: Cell Adhesion, 03 medical and health sciences, Mesenteric Veins, Cell Adhesion, medicine, Animals, education, MESH: Mice, 030304 developmental biology, MESH: Mesenteric Veins, MESH: Immunity, Natural, Macrophages, Monocyte, MESH: Macrophages, MESH: Blood Vessels, MESH: Dermis, Listeria monocytogenes, Immunity, Innate, Gene Expression Regulation, Immunology, Blood Vessels, Endothelium, Vascular, MESH: Receptors, Chemokine, 030215 immunology
Abstract: The cellular immune response to tissue damage and infection requires the recruitment of blood leukocytes. This process is mediated through a classical multistep mechanism, which involves transient rolling on the endothelium and recognition of inflammation followed by extravasation. We have shown, by direct examination of blood monocyte functions in vivo, that a subset of monocytes patrols healthy tissues through long-range crawling on the resting endothelium. This patrolling behavior depended on the integrin LFA-1 and the chemokine receptor CX 3 CR1 and was required for rapid tissue invasion at the site of an infection by this “resident” monocyte population, which initiated an early immune response and differentiated into macrophages.
Published: 2007
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29. Syk-dependent actin dynamics regulate endocytic trafficking and processing of antigens internalized through the B-cell receptor

Author: Evelyne Mougneau, Fulvia Vascotto, Bénédicte Manoury, Maria-Isabel Yuseff, Ana-Maria Lennon-Duménil, Christian Bonnerot, Gabrielle Faure-André, Delphine Le Roux, Nicolas Glaichenhaus, Danielle Lankar, Takeaki Yokozeki, Immunité et cancer, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie [Paris], Department of Biochemistry, Université de Tsukuba = University of Tsukuba, Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut national de la santé et de la recherche médicale (Inserm), Fondation pour la Recherche Médicale (FRM), Agence Nationale pour la Recherche (ANR Project JCJC06–138132), Institut Curie, Ministère de la Recherche, Association pour la Recherche sur le Cancer (ARC), Beca Presidente de la Republica from the Chilean government (Mideplan)
Subjects: MESH: Spleen, Endocytic cycle, Syk, Lymphocyte Activation, Mice, 0302 clinical medicine, hemic and lymphatic diseases, MESH: Animals, Cytoskeleton, 0303 health sciences, Antigen Presentation, biology, Antigen processing, MESH: Peptides, Intracellular Signaling Peptides and Proteins, Articles, Protein-Tyrosine Kinases, Endocytosis, Cell biology, Protein Transport, MESH: Endocytosis, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Protein Transport, MESH: Cell Line, Tumor, B-cell receptor, Antigen presentation, Receptors, Antigen, B-Cell, Major histocompatibility complex, MESH: Actins, MESH: Protein-Tyrosine Kinases, MESH: Receptors, Antigen, B-Cell, 03 medical and health sciences, Antigen, MESH: Intracellular Signaling Peptides and Proteins, Cell Line, Tumor, MESH: Cytoskeleton, Animals, Syk Kinase, MESH: Lymphocyte Activation, Molecular Biology, MESH: Mice, 030304 developmental biology, Histocompatibility Antigens Class II, Cell Biology, Actin cytoskeleton, Actins, MESH: Antigen Presentation, biology.protein, MESH: Histocompatibility Antigens Class II, Lysosomes, Peptides, Spleen, 030215 immunology, MESH: Lysosomes
Abstract: Antigen binding to the B-cell receptor (BCR) induces multiple signaling cascades that ultimately lead to B lymphocyte activation. In addition, the BCR regulates the key trafficking events that allow the antigen to reach endocytic compartments devoted to antigen processing, i.e., that are enriched for major histocompatibility factor class II (MHC II) and accessory molecules such as H2-DM. Here, we analyze the role in antigen processing and presentation of the tyrosine kinase Syk, which is activated upon BCR engagement. We show that convergence of MHC II- and H2-DM–containing compartments with the vesicles that transport BCR-uptaken antigens is impaired in cells lacking Syk activity. This defect in endocytic trafficking compromises the ability of Syk-deficient cells to form MHC II-peptide complexes from BCR-internalized antigens. Altered endocytic trafficking is associated to a failure of Syk-deficient cells to properly reorganize their actin cytoskeleton in response to BCR engagement. We propose that, by modulating the actin dynamics induced upon BCR stimulation, Syk regulates the positioning and transport of the vesicles that carry the molecules required for antigen processing and presentation.
Published: 2007
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30. Cytosolic expression of SecA2 is a prerequisite for long-term protective immunity

Author: Grégoire Lauvau, Eric Muraille, Laurel L. Lenz, Pierre Gounon, Emilie Narni-Mancinelli, Nicolas Glaichenhaus, Delphine Bassand, Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Integrated Department of Immunology, and University of Colorado [Boulder]
Subjects: MESH: Spleen, MESH: T-Lymphocyte Subsets, CD8-Positive T-Lymphocytes, medicine.disease_cause, MESH: Listeria monocytogenes, Mice, 0302 clinical medicine, Cytosol, MESH: Cytosol, T-Lymphocyte Subsets, MESH: Animals, Listeriosis, MESH: Bacterial Proteins, Adenosine Triphosphatases, 0303 health sciences, Mice, Inbred BALB C, Host cell cytosol, MESH: CD8-Positive T-Lymphocytes, 3. Good health, Cell biology, Liver, MESH: Immunologic Memory, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Listeria Infections, MESH: Mutation, Secondary infection, Immunology, MESH: Mice, Inbred BALB C, Biology, Microbiology, 03 medical and health sciences, Listeria monocytogenes, Bacterial Proteins, Immunity, Virology, medicine, MESH: Adenosine Triphosphatases, Animals, MESH: Mice, 030304 developmental biology, Pathogenic bacteria, Cytolysis, Mutation, Immunologic Memory, CD8, Spleen, 030215 immunology, MESH: Liver
Abstract: Induction of efficient adaptive T cell-mediated immunity against the intracellular bacterium Listeria monocytogenes requires its successful invasion of host cell cytosol. However, it is not clear whether its cytosolic escape and growth are sufficient to induce T cell-mediated clearance and protection upon secondary infection. To investigate this issue, we have searched for mutants that do not induce long-term protective immunity yet invade the cytosol of infected cells. We found that mice immunized with L. monocytogenes lacking the SecA2 ATPase, an auxiliary protein secretion system present in several Gram-positive pathogenic bacteria, mounted a robust cytolytic IFN-gamma-secreting CD8+ T cell response but were not protected against a secondary challenge with wild-type (wt) bacteria. Furthermore, CD8+ T cells from mice immunized with secA2- bacteria failed to transfer protection when injected into recipient mice demonstrating that they were unable to confer protection. Also, secA2- and wt L. monocytogenes spread to the same myeloid-derived cell types in vivo and SecA2 deficiency does not interfere with intracytosolic bacteria multiplication. Therefore, cytosol invasion is not sufficient for inducing secondary protective responses and induction of memory CD8+ T cells mediating long-term antibacterial protective immunity is dependent upon SecA2 expression inside the cytosol of host cells in vivo.
Published: 2007
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31. Stromal cell networks regulate lymphocyte entry, migration, and territoriality in lymph nodes

Author: Ronald N. Germain, Frédéric Brau, Nicolas Glaichenhaus, Jackson G. Egen, Lily Koo, Marc Bajénoff, Jean Pierre Laugier, Lymphocyte Biology Section ( NIAID ), National Institutes of Health, Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Commun de Microscopie Appliquée ( CCMA ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ), Institut de pharmacologie moléculaire et cellulaire ( IPMC ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Intramural Research Program of NIAID, NIH, DHHS, Institut de la Santé et de la Recherche Médicale (INSERM), Lymphocyte Biology Section (NIAID), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Commun de Microscopie Appliquée (CCMA), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Institut de pharmacologie moléculaire et cellulaire (IPMC), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Pathology, Adoptive cell transfer, Lymphocyte, MESH : Immunohistochemistry, MESH : Diagnostic Imaging, MESH : Lymphocytes, MESH: Chemotaxis, Leukocyte, MESH: Lymph Nodes, Cell Communication, Mice, 0302 clinical medicine, Reticular cell, MESH: Microscopy, Confocal, Immunology and Allergy, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, MESH: Animals, Lymphocytes, MESH : Lymph Nodes, 0303 health sciences, Microscopy, Confocal, MESH : Adoptive Transfer, Cell migration, MESH : Chemotaxis, Leukocyte, Adoptive Transfer, Immunohistochemistry, Cell biology, Chemotaxis, Leukocyte, medicine.anatomical_structure, Infectious Diseases, MESH : Microscopy, Electron, Transmission, MESH: Microscopy, Electron, Transmission, [SDV.IMM]Life Sciences [q-bio]/Immunology, Diagnostic Imaging, MESH : Microscopy, Electron, Scanning, medicine.medical_specialty, Stromal cell, Naive T cell, MESH: Microscopy, Electron, Scanning, MESH : Cell Communication, T cell, Immunology, MESH : Mice, Inbred C57BL, Biology, 03 medical and health sciences, Microscopy, Electron, Transmission, MESH: Mice, Inbred C57BL, MESH: Cell Communication, MESH : Mice, medicine, Animals, MESH : Microscopy, Confocal, MESH: Mice, 030304 developmental biology, Follicular dendritic cells, MESH: Diagnostic Imaging, MESH: Immunohistochemistry, Mice, Inbred C57BL, MESH: Adoptive Transfer, CELLIMMUNO, MESH : Stromal Cells, Microscopy, Electron, Scanning, CELLBIO, MESH: Lymphocytes, Lymph Nodes, MESH : Animals, Stromal Cells, MESH: Stromal Cells, 030215 immunology
Abstract: SummaryAfter entry into lymph nodes (LNs), B cells migrate to follicles, whereas T cells remain in the paracortex, with each lymphocyte type showing apparently random migration within these distinct areas. Other than chemokines, the factors contributing to this spatial segregation and to the observed patterns of lymphocyte movement are poorly characterized. By combining confocal, electron, and intravital microscopy, we showed that the fibroblastic reticular cell network regulated naive T cell access to the paracortex and also supported and defined the limits of T cell movement within this domain, whereas a distinct follicular dendritic cell network similarly served as the substratum for movement of follicular B cells. These results highlight the central role of stromal microanatomy in orchestrating cell migration within the LN.
Published: 2006
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32. Lymph node resident rather than skin-derived dendritic cells initiate specific T cell responses after Leishmania major infection

Author: Sem Saeland, Manfred Kopf, Anja Fröhlich, Giandomenica Iezzi, Nicolas Glaichenhaus, Bettina Ernst, Franziska Ampenberger, Institute of Integrative Biology, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Laboratory for Immunological Research, Schering-Plough, Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Epitopes, T-Lymphocyte, MESH: Lymph Nodes, MESH: T-Lymphocyte Subsets, Mice, 0302 clinical medicine, MESH: Leishmania major, T-Lymphocyte Subsets, Immunology and Allergy, MESH: Animals, 10. No inequality, Lymph node, Cells, Cultured, Leishmania major, MESH: Langerhans Cells, Antigen Presentation, Mice, Inbred BALB C, 0303 health sciences, education.field_of_study, CD11b Antigen, biology, MESH: Dendritic Cells, MESH: Antigen-Presenting Cells, 3. Good health, medicine.anatomical_structure, [SDV.IMM]Life Sciences [q-bio]/Immunology, Receptors, Chemokine, Lymph, MESH: Cells, Cultured, Langerin, MESH: Mice, Transgenic, CD8 Antigens, T cell, Immunology, Population, MESH: Mice, Inbred BALB C, Antigen-Presenting Cells, Leishmaniasis, Cutaneous, Antigens, Protozoan, Mice, Transgenic, MESH: Epitopes, T-Lymphocyte, MESH: Coculture Techniques, 03 medical and health sciences, MESH: Mice, Inbred C57BL, medicine, Animals, Antigen-presenting cell, education, MESH: Mice, 030304 developmental biology, Follicular dendritic cells, MESH: Antigens, CD11c, MESH: Antigens, CD11b, Dendritic Cells, MESH: Leishmaniasis, Cutaneous, Coculture Techniques, CD11c Antigen, Mice, Inbred C57BL, Langerhans Cells, MESH: Antigen Presentation, biology.protein, Lymph Nodes, MESH: Antigens, CD8, MESH: Receptors, Chemokine, CD8, 030215 immunology, MESH: Antigens, Protozoan
Abstract: Langerhans cells have been thought to play a major role as APCs for induction of specific immune responses to Leishmania major. Although their requirement for control of infection has been challenged recently, it remains unclear whether they can transport Ag to lymph nodes and promote initiation of T cell responses. Moreover, the role of dermal dendritic cells (DCs), another population of skin DCs, has so far not been addressed. We have investigated the origin and characterized the cell population responsible for initial activation of L. major-specific T cells in susceptible and resistant mice. We found that Ag presentation in draining lymph nodes peaks as early as 24 h after infection and is mainly mediated by a population of CD11chighCD11bhighGr-1−CD8−langerin− DCs residing in lymph nodes and acquiring soluble Ags possibly drained through the conduit network. In contrast, skin-derived DCs, including Langerhans cells and dermal DCs, migrated poorly to lymph nodes and played a minor role in early T cell activation. Furthermore, prevention of migration through early removal of the infection site did not affect Ag presentation by CD11chigh CD11bhigh DCs and activation of Leishmania major-specific naive CD4+ T cells in vivo.
Published: 2006

33. Natural killer cell behavior in lymph nodes revealed by static and real-time imaging

Author: Béatrice Breart, Hai Qi, Veronique M. Braud, Marc Bajénoff, Ronald N. Germain, Alex Yee-Chen Huang, Nicolas Glaichenhaus, Julie Cazareth, Institut de pharmacologie moléculaire et cellulaire ( IPMC ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), National Institutes of Health, Bethesda ( NIH ), National Institutes of Health ( NIH ), Université Côte d'Azur ( UCA ), Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), National Institutes of Health, Bethesda (NIH), National Institutes of Health [Bethesda] (NIH), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: CD4-Positive T-Lymphocytes, MESH: Signal Transduction, MESH: Inflammation, Cellular differentiation, MESH : Immunohistochemistry, MESH: Lymph Nodes, MESH : Leishmaniasis, Cutaneous, Lymphocyte Activation, Mice, Interleukin 21, 0302 clinical medicine, MESH: Leishmania major, Immunology and Allergy, Interferon gamma, MESH: Animals, MESH : Lymph Nodes, Leishmania major, Mice, Inbred BALB C, 0303 health sciences, MESH: CD4-Positive T-Lymphocytes, Cell Differentiation, MESH : Interferon Type II, Articles, Acquired immune system, Immunohistochemistry, MESH : Mice, Transgenic, Cell biology, Killer Cells, Natural, medicine.anatomical_structure, MESH : CD4-Positive T-Lymphocytes, MESH : Killer Cells, Natural, MESH : Cell Differentiation, Intravital microscopy, Signal Transduction, medicine.drug, MESH: Cell Differentiation, MESH: Killer Cells, Natural, MESH: Mice, Transgenic, MESH: Interferon Type II, T cell, Immunology, MESH: Mice, Inbred BALB C, Leishmaniasis, Cutaneous, Mice, Transgenic, Biology, Article, Natural killer cell, MESH : Leishmania major, Interferon-gamma, 03 medical and health sciences, MESH : Microscopy, Fluorescence, Multiphoton, MESH : Mice, medicine, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Lymphocyte Activation, MESH: Mice, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH : Lymphocyte Activation, MESH : Mice, Inbred BALB C, 030304 developmental biology, Inflammation, MESH : Signal Transduction, MESH: Humans, MESH : Inflammation, MESH : Humans, MESH: Immunohistochemistry, Dendritic cell, Cell Biology, MESH: Leishmaniasis, Cutaneous, Microscopy, Fluorescence, Multiphoton, MESH: Microscopy, Fluorescence, Multiphoton, Lymph Nodes, MESH : Animals, 030215 immunology
Abstract: Natural killer (NK) cells promote dendritic cell (DC) maturation and influence T cell differentiation in vitro. To better understand the nature of the putative interactions among these cells in vivo during the early phases of an adaptive immune response, we have used immunohistochemical analysis and dynamic intravital imaging to study NK cell localization and behavior in lymph nodes (LNs) in the steady state and shortly after infection with Leishmania major. In the LNs of naive mice, NK cells reside in the medulla and the paracortex, where they closely associate with DCs. In contrast to T cells, intravital microscopy revealed that NK cells in the superficial regions of LNs were slowly motile and maintained their interactions with DCs over extended times in the presence or absence of immune-activating signals. L. major induced NK cells to secrete interferon-γ and to be recruited to the paracortex, where concomitant CD4 T cell activation occurred. Therefore, NK cells form a reactive but low mobile network in a strategic area of the LN where they can receive inflammatory signals, interact with DCs, and regulate colocalized T cell responses.
Published: 2006
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34. Generation of functional HLA-DR*1101 tetramers receptive for loading with pathogen- or tumour-derived synthetic peptides

Author: Giulia Casorati, Maria Pia Protti, Monica Moro, Virginia Cecconi, Chiara Martinoli, Nicholas Glaichenhaus, Eliana Dallegno, Barbara Giabbai, Paolo Dellabona, Massimo Degano, Experimental Immunology Unit, Dept. of Oncology, DIBIT San Raffaele Scientific Institute, Biocrystallography Unit, Immunologie des Maladies Infectieuses Allergiques et Autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Tumour Immunology Unit, Dept. of Oncology, Monica Moro is supported by a fellowship from American Italian Cancer Foundation. The study was supported in part by funds from Associazione Italiana per la Ricerca sul Cancro (AIRC), Cancer Research Institute, Fondazione Guido Berlucchi, Compagnia di San Paolo, Fondazione Cariplo to P.D. and G.C., and Fondazione Italiana Sclerosi Multipla Onlus (2003/R/19) to M.D., Autard, Delphine, and Université Nice Sophia Antipolis (1965 - 2019) (UNS)
Subjects: CD4-Positive T-Lymphocytes, MESH: HLA-DR Antigen, Protein Conformation, MESH: Genes, Synthetic, MESH: Antigens, Neoplasm, Hemagglutinin Glycoproteins, Influenza Virus, Epitopes, 0302 clinical medicine, Genes, Synthetic, Tetanus Toxoid, MESH: Animals, HLA-DR Antigen, 0303 health sciences, Antigen Presentation, biology, Genes, Immunoglobulin, Methodology Article, MESH: CD4-Positive T-Lymphocytes, MESH: Comparative Study, MESH: Genes, MHC Class II, Cell biology, Neoplasm Proteins, medicine.anatomical_structure, Drosophila melanogaster, [SDV.IMM]Life Sciences [q-bio]/Immunology, lcsh:Immunologic diseases. Allergy, DNA, Complementary, MESH: Epitopes, [SDV.IMM] Life Sciences [q-bio]/Immunology, T cell, Recombinant Fusion Proteins, Immunology, Antigen presentation, Genes, MHC Class II, Transfection, Cell Line, MESH: Drosophila melanogaster, 03 medical and health sciences, Antigen, Antigens, Neoplasm, MHC class I, medicine, HLA-DR, Animals, Humans, Biotinylation, MESH: Biotinylation, 030304 developmental biology, MHC class II, Leucine Zippers, HLA-DR Antigens, MESH: DNA, Complementary, Molecular biology, Peptide Fragments, Immunoglobulin Fc Fragments, MESH: Cell Line, MESH: Genes, Immunoglobulin, Immunoglobulin G, MESH: Antigen Presentation, biology.protein, lcsh:RC581-607, CD8, 030215 immunology, HLA-DRB1 Chains
Abstract: BackgroundMHC class I-peptide tetramers are currently utilised to characterize CD8+T cell responses at single cell level. The generation and use of MHC class II tetramers to study antigen-specific CD4+T cells appears less straightforward. Most MHC class II tetramers are produced with a homogeneously built-in peptide, reducing greatly their flexibility of use. We attempted the generation of "empty" functional HLA-DR*1101 tetramers, receptive for loading with synthetic peptides by incubation. No such reagent is in fact available for this HLA-DR allele, one of the most frequent in the Caucasian population.ResultsWe compared soluble MHC class II-immunoglobulin fusion proteins (HLA-DR*1101-Ig) with soluble MHC class II protein fused with an optimised Bir site for enzymatic biotynilation (HLA-DR*1101-Bir), both produced in insect cells. The molecules were multimerised by binding fluorochrome-protein A or fluorochrome-streptavidin, respectively. We find that HLA-DR*1101-Bir molecules are superior to the HLA-DR*1101-Ig ones both in biochemical and functional terms. HLA-DR*1101-Bir molecules can be pulsed with at least three different promiscuous peptide epitopes, derived from Tetanus Toxoid, influenza HA and the tumour associated antigen MAGE-3 respectively, to stain specific CD4+T cells. Both staining temperature and activation state of CD4+T cells are critical for the binding of peptide-pulsed HLA-DR*1101-Bir to the cognate TCR.ConclusionIt is therefore possible to generate a soluble recombinant HLA-DR*1101 backbone that is receptive for loading with different peptides to stain specific CD4+T cells. As shown for other HLA-DR alleles, we confirm that not all the strategies to produce soluble HLA-DR*1101 multimers are equivalent.
Published: 2005
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35. iNOS-Producing Inflammatory Dendritic Cells Constitute the Major Infected Cell Type during the Chronic Leishmania major Infection Phase of C57BL/6 Resistant Mice

Author: Shizuo Akira, Yves Carlier, Carl De Trez, Bernhard Ryffel, Eric Muraille, Stefan Magez, Institute of Infectious Diseases and Molecular Medicine (IIDMM), University of Cape Town, Laboratory for Cellular and Molecular Immunology, Vrije Universiteit Brussel (VUB), Department of Host Defense, Osaka University [Osaka], Immunologie et Embryologie Moléculaires (IEM), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Parasitologie, Faculté de Médecine, Université libre de Bruxelles (ULB), Immunologie des maladies infectieuses allergiques et autoimmunes, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Cellular and Molecular Immunology, and Department of Bio-engineering Sciences
Subjects: MESH: Inflammation, Immunology/Innate Immunity, C57BL/6, Nitric Oxide Synthase Type II, MESH: Flow Cytometry, MESH: Lymph Nodes, inducible Nitric Oxide Synthase (iNOS/NOS2), Mice, MESH: Leishmania major, 0302 clinical medicine, lymph nodes, MESH: Receptors, CCR2, MESH: Animals, Leishmania major, lcsh:QH301-705.5, Lymph node, Inflammation -- enzymology -- parasitology, Mice, Inbred BALB C, MESH: Statistics, Nonparametric, MESH: Cytokines, 0303 health sciences, education.field_of_study, MESH: Dendritic Cells, Sciences bio-médicales et agricoles, Flow Cytometry, 3. Good health, Cytokines -- metabolism, Phenotype, medicine.anatomical_structure, Receptors, CCR2 -- metabolism, Cytokines, Nitric Oxide Synthase Type II -- biosynthesis -- metabolism, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Nitric Oxide Synthase Type II, MESH: Luminescent Proteins, Tumor necrosis factor alpha, Lymph, Research Article, lcsh:Immunologic diseases. Allergy, mice, Receptors, CCR2, MESH: Mice, Transgenic, Immunology, Population, MESH: Mice, Inbred BALB C, Leishmaniasis, Cutaneous, Luminescent Proteins -- genetics -- metabolism, CD11c, Mice, Transgenic, Dendritic Cells -- enzymology -- immunology -- parasitology, Biology, MESH: Phenotype, Microbiology, Statistics, Nonparametric, 03 medical and health sciences, MESH: Mice, Inbred C57BL, Immunology/Immunity to Infections, Virology, Genetics, medicine, Animals, dendritic cells, education, MESH: Mice, Molecular Biology, 030304 developmental biology, Leishmaniasis, Cutaneous -- enzymology -- immunology -- parasitology, CD40, MESH: Chronic Disease, Infectious Diseases/Protozoal Infections, MESH: Leishmaniasis, Cutaneous, biology.organism_classification, Leishmania, Mice, Inbred C57BL, Luminescent Proteins, lcsh:Biology (General), inflammation, Lymph Nodes -- metabolism, Immunology/Immune Response, Chronic Disease, biology.protein, Parasitology, Leishmania major -- immunology, lcsh:RC581-607, chronic disease, 030215 immunology
Abstract: Leishmania major parasites reside and multiply in late endosomal compartments of host phagocytic cells. Immune control of Leishmania growth absolutely requires expression of inducible Nitric Oxide Synthase (iNOS/NOS2) and subsequent production of NO. Here, we show that CD11b+ CD11c+ Ly-6C+ MHC-II+ cells are the main iNOS-producing cells in the footpad lesion and in the draining lymph node of Leishmania major-infected C57BL/6 mice. These cells are phenotypically similar to iNOS-producing inflammatory DC (iNOS-DC) observed in the mouse models of Listeria monocytogenes and Brucella melitensis infection. The use of DsRed-expressing parasites demonstrated that these iNOS-producing cells are the major infected population in the lesions and the draining lymph nodes. Analysis of various genetically deficient mouse strains revealed the requirement of CCR2 expression for the recruitment of iNOS-DC in the draining lymph nodes, whereas their activation is strongly dependent on CD40, IL-12, IFN-gamma and MyD88 molecules with a partial contribution of TNF-alpha and TLR9. In contrast, STAT-6 deficiency enhanced iNOS-DC recruitment and activation in susceptible BALB/c mice, demonstrating a key role for IL-4 and IL-13 as negative regulators. Taken together, our results suggest that iNOS-DC represent a major class of Th1-regulated effector cell population and constitute the most frequent infected cell type during chronic Leishmania major infection phase of C57BL/6 resistant mice., info:eu-repo/semantics/published
Published: 2009
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35 results on '"Immunologie des Maladies Infectieuses Allergiques et Autoimmunes"'

1. Comparative analysis of the perception of nuclear risk in two populations (expert/non-expert) in France

2. Correction: Stratification and prediction of remission in first-episode psychosis patients: the OPTiMiSE cohort study

3. The Patient-Reported Experience Measure for Improving qUality of care in Mental health (PREMIUM) project in France: study protocol for the development and implementation strategy

4. Une approche bioculturelle du premier aliment du nouveau-né. Le colostrum

5. In The Quest For Stokes V - Science Cases And Technical Challenges

6. Engraftment and in vivo proliferation advantage of gene-corrected mobilized CD34(+) cells from Fanconi anemia patients

7. Splenic CD8α+ dendritic cells undergo rapid programming by cytosolic bacteria and inflammation to induce protective CD8+ T-cell memory

8. Stem Cell Gene Therapy for Fanconi Anemia: Report from the 1st International Fanconi Anemia Gene Therapy Working Group Meeting

9. A subset of dendritic cells induces CD4+ T cells to produce IFN-γ by an IL-12–independent but CD70-dependent mechanism in vivo

10. Ongoing and prolonged response in adult low-grade gliomas treated with radiotherapy

11. Langerin+ dendritic cells are responsible for LPS-induced reactivation of allergen-specific Th2 responses in postasthmatic mice

12. Cell biology and immunology of Leishmania

13. CX3CR1 is required for airway inflammation by promoting T helper cell survival and maintenance in inflamed lung

14. The oral administration of bacterial extracts prevents asthma via the recruitment of regulatory T cells to the airways

15. Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development

16. Primed antigen-specific CD4+ T cells are required for NK cell activation in vivo upon Leishmania major infection

17. Human adenosine deaminase 2 induces differentiation of monocytes into macrophages and stimulates proliferation of T helper cells and macrophages

18. Visualizing early splenic memory CD8+ T cells reactivation against intracellular bacteria in the mouse

19. Direct Visualization of Peptide/MHC Complexes at the Surface and in the Intracellular Compartments of Cells Infected In Vivo by Leishmania major

20. CX(3)CR1(+) CD115(+) CD135(+) common macrophage/DC precursors and the role of CX(3)CR1 in their response to inflammation

21. A dynamic map of antigen recognition by CD4 T cells at the site of Leishmania major infection

22. [A new vision for autoimmunity]

23. CD4+CD25+ Tregs control the TRAIL-dependent cytotoxicity of tumor-infiltrating DCs in rodent models of colon cancer

24. Fibroblastic reticular cells guide T lymphocyte entry into and migration within the splenic T cell zone

25. Breast milk-mediated transfer of an antigen induces tolerance and protection from allergic asthma

26. Imaging host-pathogen interactions

27. Memory CD8+ T cells mediate antibacterial immunity via CCL3 activation of TNF/ROI+ phagocytes

28. Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior

29. Syk-dependent actin dynamics regulate endocytic trafficking and processing of antigens internalized through the B-cell receptor

30. Cytosolic expression of SecA2 is a prerequisite for long-term protective immunity

31. Stromal cell networks regulate lymphocyte entry, migration, and territoriality in lymph nodes

32. Lymph node resident rather than skin-derived dendritic cells initiate specific T cell responses after Leishmania major infection

33. Natural killer cell behavior in lymph nodes revealed by static and real-time imaging

34. Generation of functional HLA-DR*1101 tetramers receptive for loading with pathogen- or tumour-derived synthetic peptides

35. iNOS-Producing Inflammatory Dendritic Cells Constitute the Major Infected Cell Type during the Chronic Leishmania major Infection Phase of C57BL/6 Resistant Mice

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35 results on '"Immunologie des Maladies Infectieuses Allergiques et Autoimmunes"'

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