34,992 results on '"Immunodeficiency"'
Search Results
2. COVID-19 Vaccination in Patients with Inborn Errors of Immunity Reduces Hospitalization and Critical Care Needs Related to COVID-19: a USIDNET Report.
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McDonnell, John, Cousins, Kimberley, Younger, M, Lane, Adam, Abolhassani, Hassan, Abraham, Roshini, Al-Tamemi, Salem, Aldave-Becerra, Juan, Al-Faris, Eman, Alfaro-Murillo, Alberto, AlKhater, Suzan, Alsaati, Nouf, Doss, Alexa, Anderson, Melissa, Angarola, Ernestina, Ariue, Barbara, Arnold, Danielle, Assaad, Amal, Aytekin, Caner, Bank, Meaghan, Bergerson, Jenna, Bleesing, Jack, Boesing, John, Bouso, Carolina, Brodszki, Nicholas, Cabanillas, Diana, Cady, Carol, Callahan, Meghan, Caorsi, Roberta, Carbone, Javier, Carrabba, Maria, Castagnoli, Riccardo, Catanzaro, Jason, Chan, Samantha, Chandra, Sharat, Chapdelaine, Hugo, Chavoshzadeh, Zahra, Chong, Hey, Connors, Lori, Consonni, Filippo, Correa-Jimenez, Oscar, Cunningham-Rundles, Charlotte, DAstous-Gauthier, Katherine, Delmonte, Ottavia, Demirdag, Yesim, Deshpande, Deepti, Diaz-Cabrera, Natalie, Dimitriades, Victoria, El-Owaidy, Rasha, ElGhazali, Gehad, Al-Hammadi, Suleiman, Fabio, Giovanna, Faure, Astrid, Feng, Jin, Fernandez, James, Fill, Lauren, Franco, Guacira, Frenck, Robert, Fuleihan, Ramsay, Giardino, Giuliana, Galant-Swafford, Jessica, Gambineri, Eleonora, Garabedian, Elizabeth, Geerlinks, Ashley, Goudouris, Ekaterini, Grecco, Octavio, Pan-Hammarström, Qiang, Khani, Hedieh, Hammarström, Lennart, Hartog, Nicholas, Heimall, Jennifer, Hernandez-Molina, Gabriela, Horner, Caroline, Hostoffer, Robert, Hristova, Nataliya, Hsiao, Kuang-Chih, Ivankovich-Escoto, Gabriela, Jaber, Faris, Jalil, Maaz, Jamee, Mahnaz, Jean, Tiffany, Jeong, Stephanie, Jhaveri, Devi, Jordan, Michael, Joshi, Avni, Kalkat, Amanpreet, Kanarek, Henry, Kellner, Erinn, Khojah, Amer, Khoury, Ruby, Kokron, Cristina, Kumar, Ashish, Lecerf, Kelsey, Lehman, Heather, Leiding, Jennifer, Lesmana, Harry, Lim, Xin, Lopes, Joao, López, Ana, and Tarquini, Lucia
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Immunization ,Immunodeficiency ,Outcomes ,Viruses: respiratory diseases - Abstract
BACKGROUND: The CDC and ACIP recommend COVID-19 vaccination for patients with inborn errors of immunity (IEI). Not much is known about vaccine safety in IEI, and whether vaccination attenuates infection severity in IEI. OBJECTIVE: To estimate COVID-19 vaccination safety and examine effect on outcomes in patients with IEI. METHODS: We built a secure registry database in conjunction with the US Immunodeficiency Network to examine vaccination frequency and indicators of safety and effectiveness in IEI patients. The registry opened on January 1, 2022, and closed on August 19, 2022. RESULTS: Physicians entered data on 1245 patients from 24 countries. The most common diagnoses were antibody deficiencies (63.7%). At least one COVID-19 vaccine was administered to 806 patients (64.7%), and 216 patients received vaccination prior to the development of COVID-19. The most common vaccines administered were mRNA-based (84.0%). Seventeen patients were reported to seek outpatient clinic or emergency room care for a vaccine-related complication, and one patient was hospitalized for symptomatic anemia. Eight hundred twenty-three patients (66.1%) experienced COVID-19 infection. Of these, 156 patients required hospitalization (19.0%), 47 required ICU care (5.7%), and 28 died (3.4%). Rates of hospitalization (9.3% versus 24.4%, p
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- 2024
3. ImmunoCARE: Rapid, Accurate COVID Testing to Reduce Hospitalization of Immunocompromised Individuals
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Cue Health and Julia Moore Vogel, Program Director
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- 2024
4. Genetic Analysis of Immune Disorders
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- 2024
5. A Phase II Study of Allogeneic Hematopoietic Stem Cell Transplant for Subjects With VEXAS (Vacuoles, E1 Enzyme, X-linked, Autoinflammatory, Somatic) Syndrome
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- 2024
6. Allogeneic Hematopoietic Stem Cell Transplant for GATA2 Mutations
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- 2024
7. Allogeneic Hematopoietic Stem Cell Transplantation With JSP191-Based Conditioning in Participants With GATA2 Deficiency
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- 2024
8. Sample Collection for Systems Evaluation of Patients With Unknown or Incompletely Characterized Immune Defects
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- 2024
9. OPtimisation of Antiviral Therapy in Immunocompromised COVID-19 Patients: a Randomized Factorial Controlled Strategy Trial (OPTICOV)
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University Hospital, Geneva and Alexandra Calmy, HIV/AIDS Unit Director
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- 2024
10. SYSTEMS-LEVEL ANALYSES OF IMMUNE DYSREGULATION (SAID)
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Karolinska University Hospital, Skane University Hospital, Sahlgrenska University Hospital, Sweden, Uppsala University Hospital, Region Halland, Region Jönköping County, and Petter Brodin, M.D., Ph.D., Professor
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- 2024
11. Blood Samples to Identify Biomarkers of Busulfan
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University of Washington and Seattle Children's Hospital
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- 2024
12. Somatic RAP1B gain-of-function variant underlies isolated thrombocytopenia and immunodeficiency.
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Benavides-Nieto, Marta, Adam, Frédéric, Martin, Emmanuel, Boussard, Charlotte, Lagresle-Peyrou, Chantal, Callebaut, Isabelle, Kauskot, Alexandre, Repérant, Christelle, Miao Feng, Bordet, Jean-Claude, Castelle, Martin, Morelle, Guillaume, Brouzes, Chantal, Zarhrate, Mohammed, Panikulam, Patricia, Lambert, Nathalie, Picard, Capucine, Bodet, Damien, Rouger-Gaudichon, Jérémie, and Revy, Patrick
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HEMATOPOIETIC stem cell transplantation , *THROMBOCYTOPENIA , *BONE marrow cells , *CELL populations , *IMMUNODEFICIENCY - Abstract
The ubiquitously expressed small GTPase Ras-related protein 1B (RAP1B) acts as a molecular switch that regulates cell signaling, cytoskeletal remodeling, and cell trafficking and activates integrins in platelets and lymphocytes. The residue G12 in the P-loop is required for the RAP1B-GTPase conformational switch. Heterozygous germline RAP1B variants have been described in patients with syndromic thrombocytopenia. However, the causality and pathophysiological impact remained unexplored. We report a boy with neonatal thrombocytopenia, combined immunodeficiency, neutropenia, and monocytopenia caused by a heterozygous de novo single nucleotide substitution, c.35G>A (p.G12E) in RAP1B. We demonstrate that G12E and the previously described G12V and G60R were gain-offunction variants that increased RAP1B activation, talin recruitment, and integrin activation, thereby modifying late responses such as platelet activation, T cell proliferation, and migration. We show that in our patient, G12E was a somatic variant whose allele frequency decreased over time in the peripheral immune compartment, but remained stable in bone marrow cells, suggesting a differential effect in distinct cell populations. Allogeneic hematopoietic stem cell transplantation fully restored the patient’s hemato-immunological phenotype. Our findings define monoallelic RAP1B gain-of-function variants as a cause for constitutive immunodeficiency and thrombocytopenia. The phenotypic spectrum ranged from isolated hematological manifestations in our patient with somatic mosaicism to complex syndromic features in patients with reported germline RAP1B variants. [ABSTRACT FROM AUTHOR]
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- 2024
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13. MicroRNA dysregulation in ataxia telangiectasia.
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Cirillo, Emilia, Tarallo, Antonietta, Toriello, Elisabetta, Carissimo, Annamaria, Giardino, Giuliana, De Rosa, Antonio, Damiano, Carla, Soresina, Annarosa, Badolato, Raffaele, Dellepiane, Rosa Maria, Baselli, Lucia A., Carrabba, Maria, Fabio, Giovanna, Bertolini, Patrizia, Montin, Davide, Conti, Francesca, Romano, Roberta, Pozzi, Elisa, Ferrero, Giulio, and Roncarati, Roberta
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GENE expression ,MONONUCLEAR leukocytes ,ATAXIA telangiectasia ,DNA repair ,BLOOD testing - Abstract
Introduction: Ataxia telangiectasia (AT) is a rare disorder characterized by neurodegeneration, combined immunodeficiency, a predisposition to malignancies, and high clinical variability. Profiling of microRNAs (miRNAs) may offer insights into the underlying mechanisms of complex rare human diseases, as miRNAs play a role in various biological functions including proliferation, differentiation, and DNA repair. In this study, we investigate the differential expression of miRNAs in samples from AT patients to identify miRNA patterns and analyze how these patterns are related to the disease. Methods: We enrolled 20 AT patients (mean age 17.7 ± 9.6 years old) and collected clinical and genetic data. We performed short non-coding RNA-seq analysis on peripheral blood mononuclear cells (PBMCs) and fibroblasts to compare the miRNA expression profile between AT patients and controls. Results: We observed 42 differentially expressed (DE)-miRNAs in blood samples and 26 in fibroblast samples. Among these, three DE-miRNAs, miR-342-3p, miR30a-5p, and miR-195-5p, were further validated in additional AT samples, confirming their dysregulation. Discussion: We identified an AT-related miRNA signature in blood cells and fibroblast samples collected from a group of AT patients. We also predicted several dysregulated pathways, primarily related to cancer, immune system control, or inflammatory processes. The findings suggest that miRNAs may provide insights into the pathophysiology and tumorigenesis of AT and have the potential to serve as useful biomarkers in cancer research. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Screening for primary immune deficiency among patients with bronchiectasis.
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Dufrénoy, Mylène, Luca, Luminita, Bironneau, Vanessa, Meurice, Jean-Claude, Puyade, Mathieu, and Martin, Mickaël
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IMMUNODEFICIENCY , *BRONCHIECTASIS , *IMMUNOGLOBULINS , *BLOOD protein electrophoresis , *ANTIBIOTICS - Abstract
To assess frequency and methods of PID (primary immune deficiency) screening among patients with bronchiectasis by pneumologists in clinical practice. All the patients hospitalized in the department of pneumology of the Poitiers University Hospital between April 2013 and April 2020 with a diagnosis of bronchiectasis on chest computerized tomography were included. Patients aged 70 and over and those with already known PID were excluded. Primary endpoint was the proportion of patients having had serum immunoglobulin (Ig) assay and serum protein electrophoresis (SPE) analysis. Secondary endpoints were factors associated with prescription of SPE and/or Ig assay, proportion of patients with newly diagnosed PID and their characteristics and factors associated with repeated courses of antibiotics. Among the 133 patients included, 43% had SPE + Ig assay, 34% SPE only and 23% neither. The proportion of patients with asthma was higher in the "SPE + Ig assay" group (33.3%) compared to the "SPE only" (11.1%) and the "Neither SPE nor Ig assay" groups (6.4%) (P = 0.002). Four patients were newly diagnosed for PID of whom 3 had subclass IgG deficiency. Factors associated with repeated courses of antibiotics were generalized bronchiectasis (P = 0.02) and asthma (P = 0.04). PID is underscreened by pneumologists among patients with bronchiectasis. Association of SPE + Ig assay + IgG subclass assay appears as the most accurate combination. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Deficiency of Adenosine Deaminase 2.
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Coşkun, Çağrı and Ünal, Şule
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STROKE prevention , *HYDROLASES , *VASCULITIS , *ANTI-inflammatory agents , *HEMATOPOIETIC stem cell transplantation , *GENE therapy , *PRIMARY immunodeficiency diseases , *ANEMIA , *NEUTROPHILS , *ADENOSINES , *AUTOINFLAMMATORY diseases , *RECOMBINANT proteins , *GENETIC mutation , *CYTOKINES , *TUMOR necrosis factors , *PHENOTYPES , *SYMPTOMS - Abstract
Adenosine deaminase 2 (ADA2) deficiency is an autosomal recessively inherited autoinflammatory disorder caused by loss-of-function mutations in the ADA2 gene. Although the pathogenesis involves the triggering of a proinflammatory cascade due to increased production of inflammatory cytokines such as tumor necrosis factor (TNF)-α and dysregulation of neutrophil extracellular trap formation resulting from an excess accumulation of extracellular adenosine, the pathogenetic mechanism still needs further clarification due to the broad clinical spectrum. In addition to the initially described vasculitis-related symptoms, hematological, immunological, and autoinflammatory symptoms are now well recognized. The diagnosis is made by demonstration of pathogenic variants of ADA2 with biallelic loss of function and identification of low plasma ADA2 catalytic activity. Currently, TNF-α inhibitors are the treatment of choice for controlling vasculitis manifestations and preventing strokes. However, in patients presenting with severe hematologic findings, TNF-α inhibitors are not the treatment of choice and hematopoietic stem cell transplantation has been shown to be successful in selected cases. Recombinant ADA2 protein and gene therapy are promising treatment modalities for the future. In conclusion, ADA2 deficiency has a broad phenotype and should be considered in the differential diagnosis of different clinical situations. In this review, we summarize the disease manifestations of ADA2 deficiency and available treatment options. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Negative immune regulation contributes to disease tolerance in Drosophila melanogaster.
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Prakash, Arun, Monteith, Katy M., and Vale, Pedro F.
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DROSOPHILA melanogaster , *IMMUNODEFICIENCY , *ANTIMICROBIAL peptides , *IMMUNOLOGICAL tolerance , *IMMUNOPATHOLOGY - Abstract
Disease tolerance is an infection phenotype where hosts show relatively high health despite harbouring elevated pathogen loads. Variation in the ability to reduce immunopathology may explain why some hosts can tolerate higher pathogen burdens with reduced pathology. Negative immune regulation would therefore appear to be a clear candidate for a mechanism underlying disease tolerance. Here, we examined how the negative regulation of the immune deficiency (IMD) pathway affects disease tolerance in Drosophila melanogaster when infected with four doses of the gram‐negative bacterial pathogen Pseudomonas entomophila. We find that while flies unable to regulate the IMD response exhibited higher expression of antimicrobial peptides and lower bacterial loads as expected, this was not accompanied by a proportional reduction in mortality. Instead, ubiquitous UAS‐RNAi knockdown of negative regulators of IMD (pirk and caudal) substantially increased the per‐pathogen‐mortality in both males and females across all tested infectious doses. Our results therefore highlight that in addition to regulating an efficient pathogen clearance response, negative regulators of IMD also contribute to disease tolerance. [ABSTRACT FROM AUTHOR]
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- 2024
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17. Torque Teno Virus Load Is Associated With Centers for Disease Control and Prevention Stage and CD4+ Cell Count in People Living With Human Immunodeficiency Virus but Seems Unrelated to AIDS-Defining Events and Human Pegivirus Load.
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Esser, Pia L, Quintanares, Gibran H Rubio, Langhans, Bettina, Heger, Eva, Böhm, Michael, Jensen, Björn-Erik O L E, Esser, Stefan, Lübke, Nadine, Fätkenheuer, Gerd, Lengauer, Thomas, Klein, Florian, Oette, Mark, Rockstroh, Juergen K, Boesecke, Christoph, Cristanziano, Veronica Di, Kaiser, Rolf, and Pirkl, Martin
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TORQUE teno virus , *HIV infections , *HIV , *HIV-positive persons , *BIOMARKERS - Abstract
Background Torque teno virus (TTV) is part of the human virome. TTV load was related to the immune status in patients after organ transplantation. We hypothesize that TTV load could be an additional marker for immune function in people living with HIV (PLWH). Methods In this analysis, serum samples of PLWH from the RESINA multicenter cohort were reanalyzed for TTV. Investigated clinical and epidemiological parameters included human pegivirus load, patient age and sex, HIV load, CD4+ T-cell count (Centers for Disease Control and Prevention [CDC] stage 1, 2, or 3), and CDC clinical stage (1993 CDC classification system; stage A, B, or C) before initiation of antiretroviral therapy. Regression analysis was used to detect possible associations among parameters. Results Our analysis confirmed TTV as a strong predictor of CD4+ T-cell count and CDC class 3. This relationship was used to propose a first classification of TTV load with regard to clinical stage. We found no association with clinical CDC stages A–C. The human pegivirus load was inversely correlated with HIV load but not TTV load. Conclusions TTV load was associated with immunodeficiency in PLWH. Neither TTV nor HIV load were predictive for the clinical categories of HIV infection. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Case report: Macrophage activation syndrome in a patient with Kabuki syndrome.
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Jingyuan Zhang, Yuanbo Kang, Zenan Xia, Yuming Chong, Xiao Long, and Min Shen
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MACROPHAGE activation syndrome ,CONGENITAL disorders ,T cells ,METHYLTRANSFERASES ,LYSINE - Abstract
Macrophage activation syndrome (MAS), is a severe and fatal complication of various pediatric inflammatory disorders. Kabuki syndrome (KS), mainly caused by lysine methyltransferase 2D (KMT2D; OMIM 602113) variants, is a rare congenital disorder with multi-organ deficiencies. To date, there have been no reported cases of MAS in patients with KS. This report describes a case of a 22-year-old male with Kabuki syndrome (KS) who developed MAS. This unique case not only deepens the understanding of the involvement of KMT2D in immune regulation and disease, but expands the phenotype of the adult patient to better understand the natural history, disease burden, and management of patients with KS complicated with autoimmune disorders. [ABSTRACT FROM AUTHOR]
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- 2024
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19. ADA2 Eksikliği.
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Köse, Hülya and Kılıç, Sara Şebnem
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HYDROLASES , *VASCULITIS , *ANTI-inflammatory agents , *HEMATOPOIETIC stem cell transplantation , *IMMUNOLOGICAL deficiency syndromes , *AUTOINFLAMMATORY diseases , *CHROMOSOME abnormalities , *APLASTIC anemia , *PHENOTYPES , *POLYARTERITIS nodosa - Abstract
Deficiency of Adenosine Deaminase 2 (DADA2) is a systemic disorder that is inherited in an autosomal recessive manner. Initially identified as a monogenic form of systemic vasculitis that mirrors polyarteritis nodosa (PAN), DADA2 is attributed to biallelic pathogenic variants in the adenosine deaminase 2 gene (ADA2; formerly known as CECR1) on chromosome 22q11. DADA2 presents with variable symptoms that can manifest as vasculitis, immune dysfunction, or hematological abnormalities. The primary disease phenotypes consist of polyarteritis nodosa (PAN)-like vasculitis, Diamond-Blackfan anemia (DBA)-like hematologic findings, and immunodeficiency. The vasculitic condition often manifests through severe ischemic or hemorrhagic strokes that can be life-threatening. To confirm the diagnosis of DADA2, it is imperative to analyze the ADA2 gene and measure low or unnoticeable ADA2 activity in plasma or serum. The use of anti-tumor necrosis factor agents has proven to be efficacious in treating patients with an inflammatory phenotype. Hematopoietic stem cell transplantation (HSCT) stands out as a promising treatment alternative for patients presenting with predominant hematologic or immunologic manifestations. [ABSTRACT FROM AUTHOR]
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- 2024
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20. Enzyme Is the Name—Adapter Is the Game.
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Huber, Michael and Brummer, Tilman
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BRUTON tyrosine kinase , *SCAFFOLD proteins , *MITOGEN-activated protein kinases , *PHOSPHATIDYLINOSITOL 3-kinases , *CATALYTIC domains - Abstract
Signaling proteins in eukaryotes usually comprise a catalytic domain coupled to one or several interaction domains, such as SH2 and SH3 domains. An additional class of proteins critically involved in cellular communication are adapter or scaffold proteins, which fulfill their purely non-enzymatic functions by organizing protein–protein interactions. Intriguingly, certain signaling enzymes, e.g., kinases and phosphatases, have been demonstrated to promote particular cellular functions by means of their interaction domains only. In this review, we will refer to such a function as "the adapter function of an enzyme". Though many stories can be told, we will concentrate on several proteins executing critical adapter functions in cells of the immune system, such as Bruton´s tyrosine kinase (BTK), phosphatidylinositol 3-kinase (PI3K), and SH2-containing inositol phosphatase 1 (SHIP1), as well as in cancer cells, such as proteins of the rat sarcoma/extracellular signal-regulated kinase (RAS/ERK) mitogen-activated protein kinase (MAPK) pathway. We will also discuss how these adaptor functions of enzymes determine or even undermine the efficacy of targeted therapy compounds, such as ATP-competitive kinase inhibitors. Thereby, we are highlighting the need to develop pharmacological approaches, such as proteolysis-targeting chimeras (PROTACs), that eliminate the entire protein, and thus both enzymatic and adapter functions of the signaling protein. We also review how genetic knock-out and knock-in approaches can be leveraged to identify adaptor functions of signaling proteins. [ABSTRACT FROM AUTHOR]
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- 2024
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21. A case report of empyema caused by Enterococcus gallinarum.
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Liu, Min, Liu, Jixiang, Wu, Juanjuan, Liu, Shuang, Sun, Lu, Li, Fajiu, and Li, Chenghong
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Background: Enterococcus gallinarum is an infrequently intestinal symbiotic pathogen associated with nosocomial infection in immunocompromised individuals. To date, rare cases of pulmonary infection attributable to Enterococcus gallinarum were reported. Herein, we presented the first case of empyema resulting from Enterococcus gallinarum infection. Case presentation: An 81-year-old male presented with fever and dyspnea upon admission. Chest CT scan and thoracic ultrasonography confirmed the presence of right pleural effusion. Thoracoscopy revealed extensive adhesion, purulent fluid, and necrotic materials within the thoracic cavity. Enterococcus gallinarum was identified through pleural effusion culture. The patient underwent an intrathoracic injection of urokinase along with thoracic drainage. Following surgery, He took oral linezolid for over one month. Undergoing comprehensive treatment, the patient exhibited favorable recovery. Conclusions: We reported the first case of empyema due to Enterococcus gallinarum infection. It should be suspected in patients with impaired immune function and invasive therapies, without responding to conventional anti-infectious treatment. [ABSTRACT FROM AUTHOR]
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- 2024
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22. Successful bone marrow transplantation in a patient with Omenn syndrome, a rare variant of severe combined immunodeficiency syndrome: A case report.
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Khan, Ubaid, Umer, Muhammad, Muhammad, Aiman, Iltaf, Arej, and Nashwan, Abdulqadir J.
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BONE marrow transplantation , *RESPIRATORY infections , *IMMUNOLOGICAL deficiency syndromes , *GENETIC disorder diagnosis , *GRAFT rejection - Abstract
Key Clinical Message: Bone marrow transplantation (BMT) saves lives in Omenn syndrome, a severe immunodeficiency disorder. Timely genetic diagnosis and matched donor BMT are crucial. Emphasis on newborn screening and multidisciplinary care improves outcomes for infants with inherited disorders. Prompt intervention and comprehensive management are vital for a successful transplant outcome. Omenn syndrome represents a severe variant of combined immunodeficiency characterized by disregulated immune responses and susceptibility to recurrent infections. We present the case of a 3‐month‐old female infant initially presenting with upper respiratory infection symptoms and a diffuse rash, unresponsive to local treatment. At 4 months of age, the patient underwent allogeneic bone marrow transplantation (BMT) utilizing stem cells from a fully matched sibling donor. Pre‐transplant conditioning included antimicrobial prophylaxis and supportive therapies. Following BMT, the patient received immunosuppressive medications to prevent graft rejection and graft‐versus‐host disease. Clinical monitoring post‐transplant showed timely neutrophil and platelet engraftment, with subsequent follow‐up demonstrating stable clinical parameters and negative cytomegalovirus status. The case highlights the importance of timely diagnosis and treatment in managing severe immunodeficiency disorders, demonstrating the potential for successful outcomes with appropriate timely interventions. Regular monitoring and follow‐up appointments were crucial in ensuring the success of the treatment. This case also emphasizes the significance of multidisciplinary care and genetic testing in identifying and managing rare immunodeficiency disorders. The successful outcome in this case provides hope for improved treatment options and better patient outcomes in the future. [ABSTRACT FROM AUTHOR]
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- 2024
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23. Unveiling the value of C‐reactive protein as a severity biomarker and the IL4/IL13 pathway as a therapeutic target in recessive dystrophic epidermolysis bullosa: A multiparametric cross‐sectional study.
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Quintana‐Castanedo, Lucía, Sánchez‐Ramón, Silvia, Maseda, Rocío, Illera, Nuria, Pérez‐Conde, Isabel, Molero‐Luis, Marta, Butta, Nora, Arias‐Salgado, Elena G., Monzón‐Manzano, Elena, Zuluaga, Pilar, Martínez‐Santamaría, Lucía, Fernández‐Arquero, Miguel, Llames, Sara G., Meana, Álvaro, de Lucas, Raúl, del Río, Marcela, Vicente, Ángeles, Escámez, María José, and Sacedón, Rosa
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IMMUNOCOMPETENCE , *IMMUNOGLOBULINS , *DRUG target , *STAPHYLOCOCCUS aureus , *PATHOLOGICAL physiology , *EPIDERMOLYSIS bullosa - Abstract
Patients with recessive dystrophic epidermolysis bullosa (RDEB) experience numerous complications, which are exacerbated by inflammatory dysregulation and infection. Understanding the immunological mechanisms is crucial for selecting medications that balance inflammation control and immunocompetence. In this cross‐sectional study, aiming to identify potential immunotherapeutic targets and inflammatory biomarkers, we delved into the interrelationship between clinical severity and systemic inflammatory parameters in a representative RDEB cohort. Encompassing 84 patients aged 1–67 and spanning all three Epidermolysis Bullosa Disease Activity and Scarring Index (EBDASI) severity categories, we analysed the interrelationship of infection history, standard inflammatory markers, systemic cytokines and Ig levels to elucidate their roles in RDEB pathophysiology. Our findings identify C‐reactive protein as an excellent biomarker for disease severity in RDEB. A type 2 inflammatory profile prevails among moderate and severe RDEB patients, correlating with dysregulated circulating IgA and IgG. These results underscore the IL4/IL13 pathways as potential evidence‐based therapeutic targets. Moreover, the complete inflammatory scenario aligns with Staphylococcus aureus virulence mechanisms. Concurrently, abnormalities in IgG, IgE and IgM levels suggest an immunodeficiency state in a substantial number of the cohort's patients. Our results provide new insights into the interplay of infection and immunological factors in the pathogenesis of RDEB. [ABSTRACT FROM AUTHOR]
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- 2024
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24. Variable Syndromic Immunodeficiency in Patients with Biallelic PRIM1 Mutations.
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Toskov, Vasil, Kaiser-Labusch, Petra, Lee-Kirsch, Min Ae, Wolf, Christine, Speckmann, Carsten, Ehl, Stephan, and Wegehaupt, Oliver
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TYPE I interferons , *B cells , *CRYPTORCHISM , *IMMUNODEFICIENCY , *DNA polymerases , *CIRRHOSIS of the liver - Abstract
Mutations in genes of the DNA polymerase complex have been linked to impaired immunological function next to distinct syndromic features. Biallelic mutations in PRIM1 are associated with a primordial dwarfism syndrome with variable hypogammaglobulinemia. The disease is mostly lethal in infancy due to pulmonary infections as well as hepatic cirrhosis. We studied 3 novel patients with PRIM1-deficiency with a focus on immunological consequences. All three shared dysmorphic features including a prominent forehead, triangular face and bilateral cryptorchidism. P1 carried the novel homozygous PRIM1 splice variant c.103+2T>G, allowing residual protein expression and associated with a mild clinical phenotype. P2 and P3 carried the known homozygous variant c.638+36C>G and died in infancy. Paradoxically, B cell lymphopenia was most pronounced in P1. No other significant lymphocyte abnormalities were detected. Interestingly, all 3 patients showed variable, but intermittently excessive Type I interferon signatures. In summary, the B-cell deficiency in PRIM1-deficiency is markedly variable and the severity of syndromic manifestations is not predictive of the immunological phenotype. We highlight a potential contribution of pathological type I interferon activation to disease pathogenesis which warrants further investigations. [ABSTRACT FROM AUTHOR]
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- 2024
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25. IFIH1 loss of function predisposes to inflammatory and SARS‐CoV‐2‐related infectious diseases.
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Najm, Rania, Yavuz, Lemis, Jain, Ruchi, El Naofal, Maha, Ramaswamy, Sathishkumar, Abuhammour, Walid, Loney, Tom, Nowotny, Norbert, Alsheikh‐Ali, Alawi, Abou Tayoun, Ahmad, and Kandasamy, Richard K.
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MULTISYSTEM inflammatory syndrome in children , *COMMUNICABLE diseases , *INFLAMMATORY bowel diseases , *AGAMMAGLOBULINEMIA - Abstract
The IFIH1 gene, encoding melanoma differentiation‐associated protein 5 (MDA5), is an indispensable innate immune regulator involved in the early detection of viral infections. Previous studies described MDA5 dysregulation in weakened immunological responses, and increased susceptibility to microbial infections and autoimmune disorders. Monoallelic gain‐of‐function of the IFIH1 gene has been associated with multisystem disorders, namely Aicardi–Goutieres and Singleton–Merten syndromes, while biallelic loss causes immunodeficiency. In this study, nine patients suffering from recurrent infections, inflammatory diseases, severe COVID‐19 or multisystem inflammatory syndrome in children (MIS‐C) were identified with putative loss‐of‐function IFIH1 variants by whole‐exome sequencing. All patients revealed signs of lymphopaenia and an increase in inflammatory markers, including CRP, amyloid A, ferritin and IL‐6. One patient with a pathogenic homozygous variant c.2807+1G>A was the most severe case showing immunodeficiency and glomerulonephritis. The c.1641+1G>C variant was identified in the heterozygous state in patients suffering from periodic fever, COVID‐19 or MIS‐C, while the c.2016delA variant was identified in two patients with inflammatory bowel disease or MIS‐C. There was a significant association between IFIH1 monoallelic loss of function and susceptibility to infections in males. Expression analysis showed that PBMCs of one patient with a c.2016delA variant had a significant decrease in ISG15, IFNA and IFNG transcript levels, compared to normal PBMCs, upon stimulation with Poly(I:C), suggesting that MDA5 receptor truncation disrupts the immune response. Our findings accentuate the implication of rare monogenic IFIH1 loss‐of‐function variants in altering the immune response, and severely predisposing patients to inflammatory and infectious diseases, including SARS‐CoV‐2‐related disorders. [ABSTRACT FROM AUTHOR]
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- 2024
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26. Metagenomic next-generation sequencing as a diagnostic tool in the clinical routine of an infectious diseases department: a retrospective cohort study.
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Kalbitz, Sven, Ermisch, Jörg, Kellner, Nils, Nickel, Olaf, Borte, Stephan, Marx, Kathrin, and Lübbert, Christoph
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COMMUNICABLE disease diagnosis ,COMMUNICABLE diseases ,BLOOD ,GENOMICS ,BLOOD collection ,POLYMERASE chain reaction ,FISHER exact test ,RETROSPECTIVE studies ,DESCRIPTIVE statistics ,STREPTOCOCCUS ,QUANTITATIVE research ,MANN Whitney U Test ,CHI-squared test ,LONGITUDINAL method ,CELL culture ,ANTI-infective agents ,MEDICAL records ,ACQUISITION of data ,COMPARATIVE studies ,DATA analysis software ,SEQUENCE analysis ,MEDICAL practice ,MICROBIAL genetics - Abstract
Background: Metagenomic next-generation sequencing (mNGS) of circulating cell-free DNA from plasma is a hypothesis-independent broadband diagnostic method for identification of potential pathogens. So far, it has only been investigated in special risk populations (e.g. patients with neutropenic fever). Purpose: To investigate the extent to which mNGS (DISQVER® platform) can be used in routine clinical practice. Methods: We collected whole blood specimens for mNGS testing, blood cultures (BC), and pathogen-specific PCR diagnostics. Clinical data and pathogen diagnostics were retrospectively reviewed by an infectious disease expert panel regarding the adjustment of anti-infective therapy. Results: In 55 selected patients (median age 53 years, 67% male) with heterogeneous diagnoses, a total of 66 different microorganisms and viruses were detected using mNGS (51% viruses, 38% bacteria, 8% fungi, 3% parasites). The overall positivity rate of mNGS was 53% (29/55). Fifty-two out of 66 (79%) potential pathogens detected by mNGS were found in patients with primary or secondary immunodeficiency. The concordance rates of BC and pathogen-specific PCR diagnostics with mNGS testing were 14% (4/28) and 36% (10/28), respectively (p < 0.001). An additional bacterial pathogen (Streptococcus agalactiae) could only be detected by BC. Therapeutic consequences regarding anti-infective therapy were drawn from 23 pathogens (35% of detections), with 18 of these detections occurring in patients with immunodeficiency. Conclusions: We conclude that mNGS is a useful diagnostic tool, but should only be performed selectively in addition to routine diagnostics of infectious diseases. The limited number of patients and the retrospective study design do not allow any further conclusions. [ABSTRACT FROM AUTHOR]
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- 2024
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27. Pharmacotherapy for CD55 deficiency with CHAPLE disease: how close are we to a cure?
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Can, Salim, Yorgun Altunbas, Melek, and Ozen, Ahmet
- Subjects
IMMUNODEFICIENCY ,CD55 antigen ,GENETIC disorders ,DRUG therapy ,ECULIZUMAB - Published
- 2024
- Full Text
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28. Immunoglobulin class‐switch recombination: Mechanism, regulation, and related diseases.
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Liu, Jia‐Chen, Zhang, Ke, Zhang, Xu, Guan, Fei, Zeng, Hu, Kubo, Masato, Lee, Pamela, Candotti, Fabio, James, Louisa Katherine, Camara, Niels Olsen Saraiva, Benlagha, Kamel, Lei, Jia‐Hui, Forsman, Huamei, Yang, Lu, Xiao, Wei, Liu, Zheng, and Liu, Chao‐Hong
- Subjects
JOB'S syndrome ,CYTIDINE deaminase ,PRIMARY immunodeficiency diseases ,THERAPEUTICS ,MULTIPLE myeloma - Abstract
Maturation of the secondary antibody repertoire requires class‐switch recombination (CSR), which switches IgM to other immunoglobulins (Igs), and somatic hypermutation, which promotes the production of high‐affinity antibodies. Following immune response or infection within the body, activation of T cell‐dependent and T cell‐independent antigens triggers the activation of activation‐induced cytidine deaminase, initiating the CSR process. CSR has the capacity to modify the functional properties of antibodies, thereby contributing to the adaptive immune response in the organism. Ig CSR defects, characterized by an abnormal relative frequency of Ig isotypes, represent a rare form of primary immunodeficiency. Elucidating the molecular basis of Ig diversification is essential for a better understanding of diseases related to Ig CSR defects and could provide clues for clinical diagnosis and therapeutic approaches. Here, we review the most recent insights on the diversification of five Ig isotypes and choose several classic diseases, including hyper‐IgM syndrome, Waldenström macroglobulinemia, hyper‐IgD syndrome, selective IgA deficiency, hyper‐IgE syndrome, multiple myeloma, and Burkitt lymphoma, to illustrate the mechanism of Ig CSR deficiency. The investigation into the underlying mechanism of Ig CSR holds significant potential for the advancement of increasingly precise diagnostic and therapeutic approaches. [ABSTRACT FROM AUTHOR]
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- 2024
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29. Central Nervous System Histoplasmosis After Acute COVID-19 in An Adolescent.
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Mayhew, Jonathan A., Tat, Kiet, Harris, Matthew E., Wheat, Joseph, Christenson, John C., and Wood, James B.
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HISTOPLASMOSIS ,SARS disease ,CORONAVIRUS diseases ,IMMUNODEFICIENCY ,MYCOSES - Abstract
Central nervous system histoplasmosis is a serious complication of a common endemic mycosis, but it is rare in immunocompetent hosts. SARS-CoV-2 has introduced significant challenges into the healthcare setting with overlapping clinical presentations that may delay the diagnosis of alternative conditions. Additionally, it may lead to immune dysregulation and increase the risk for secondary infections, including invasive fungal diseases. Limited reports have described disseminated histoplasmosis in adults associated with COVID-19, but none have described central nervous system infection or complications in pediatric patients. We report a case of disseminated histoplasmosis involving the central nervous system in a previously healthy 13-year-old male with SARS-CoV-2 infection. An extensive immunological evaluation did not identify an underlying immunodeficiency. We highlight the potential of COVID-19 immune dys-regulation to contribute to the development or progression of invasive fungal disease. [Pediatr Ann. 2024;53(8):e305–e309.] [ABSTRACT FROM AUTHOR]
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- 2024
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30. Pitfalls in time-to-event analysis of registry data: a tutorial based on simulated and real cases.
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Alligon, Mickaël, Mahlaoui, Nizar, and Bouaziz, Olivier
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MEDICAL care , *MEDICAL personnel , *IMMUNODEFICIENCY , *MEDICAL research , *SURVIVAL analysis (Biometry) - Abstract
Survival analysis (also referred to as time-to-event analysis) is the study of the time elapsed from a starting date to some event of interest. In practice, these analyses can be challenging and, if methodological errors are to be avoided, require the application of appropriate techniques. By using simulations and real-life data based on the French national registry of patients with primary immunodeficiencies (CEREDIH), we sought to highlight the basic elements that need to be handled correctly when performing the initial steps in a survival analysis. We focused on non-parametric methods to deal with right censoring, left truncation, competing risks, and recurrent events. Our simulations show that ignoring these aspects induces a bias in the results; we then explain how to analyze the data correctly in these situations using non-parametric methods. Rare disease registries are extremely valuable in medical research. We discuss the application of appropriate methods for the analysis of time-to-event from the CEREDIH registry. The objective of this tutorial article is to provide clinicians and healthcare professionals with better knowledge of the issues facing them when analyzing time-to-event data. [ABSTRACT FROM AUTHOR]
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- 2024
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31. Centralized care model for hereditary angioedema overcomes geographical barriers.
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Holmes, Ashley, Srinivasan, Cindy, Borle, Jack, Blain, Heather, Ritchie, Bruce, and Adatia, Adil
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RURAL medicine ,POISSON regression ,ANGIONEUROTIC edema ,ODDS ratio ,LOGISTIC regression analysis - Abstract
Hereditary angioedema due to C1 inhibitor deficiency (HAE) is a rare inborn error of immunity that presents with episodic swelling. Management is multifaceted and includes on-demand treatment of swelling episodes, short-term prophylaxis to prevent swelling episodes from procedures, and long-term prophylaxis (LTP) to prevent angioedema on an ongoing basis. All approved on-demand therapies are parenteral, necessitating patient training for home administration, particularly intravenous C1 inhibitor. These complexities can result in care gaps for rural HAE patients. We conducted a cross-sectional study at our Angioedema Center of Reference and Excellence to assess the care provided to urban and rural patients. The proportion of patients receiving LTP, proportion of patients diagnosed as children, and disease control measured using the Angioedema Control Test (AECT) were collected. Logistic and Poisson regression models adjusted for age and sex were used to compare the two groups. The proportion using LTP was similar at 62% and 61% in urban and rural patients, respectively (odds ratio [OR] 1.01 (CI 95% 0.34-2.99)). Among urban patients, 52% were diagnosed as children compared to 60% among rural residents (1.43 (0.37-5.56)). The mean (IQR) AECT score was 14.0 (8.5-15.5) in urban patients and 13.0 (10.0-14.0) in rural patients (Poisson β -0.001 (-0.23-0.23). These data indicate that rural patients received similar high-quality care. We attribute these findings to the centralized care model employed in which HAE patients in the region are seen at a single comprehensive care clinic. [ABSTRACT FROM AUTHOR]
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- 2024
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32. Presumptive Cytomegalovirus Retinitis as a Complication of Dyskeratosis Congenita: A Case Report.
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Du, Yuxi and Dang, Yalong
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- *
CYTOMEGALOVIRUSES , *PNEUMOCYSTIS pneumonia , *ORAL mucosa , *ORAL leukoplakia , *GENETIC disorders , *NAIL diseases , *HYPERPIGMENTATION - Abstract
Dyskeratosis congenita is a rare genetic disorder characterized by abnormalities of the skin, nails, and oral mucosa. Retinal involvement in this condition is uncommon. Here, we present a case of a young male patient diagnosed with presumptive cytomegalovirus retinitis, ultimately found to be concomitant with dyskeratosis congenita.Introduction: A non-HIV-infected young male with recurrent infections, including aspergillus pneumonia and pneumocystis pneumonia, presented with presumptive cytomegalovirus retinitis in both eyes. Systemic manifestations included cutaneous hyperpigmentation, nail dystrophy, and oral mucosal leukoplakia. Genetic testing revealed a mutation in the DKC1 gene. The final diagnosis was dyskeratosis congenita complicated by presumptive cytomegalovirus retinitis.Case Presentation: Cytomegalovirus retinitis can serve as an ocular complication of dyskeratosis congenita. When a patient presents with cytomegalovirus retinitis, a comprehensive systematic examination should be conducted as it indicates severe immunodeficiency. [ABSTRACT FROM AUTHOR]Conclusion: - Published
- 2024
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33. Tuberculosis Disease in Immunocompromised Children and Adolescents: A Pediatric Tuberculosis Network European Trials Group Multicenter Case-control Study.
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Rodríguez-Molino, Paula, Tebruegge, Marc, Noguera-Julian, Antoni, Neth, Olaf, Fidler, Katy, Brinkmann, Folke, Sainz, Talia, Ivaskeviciene, Inga, Ritz, Nicole, Brito, Maria Joao, Silva, Tiago Milheiro, Chechenieva, Vira, Serdiuk, Maryna, Lancella, Laura, Russo, Cristina, Soler-García, Aleix, Navarro, Maria Luisa, Krueger, Renate, Feiterna-Sperling, Cornelia, and Starshinova, Anna
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TUBERCULOSIS diagnosis , *PREVENTION of communicable diseases , *TUBERCULOSIS epidemiology , *HIV infection complications , *RISK assessment , *DISEASE duration , *RESEARCH funding , *IMMUNOCOMPROMISED patients , *TREATMENT effectiveness , *DESCRIPTIVE statistics , *ANTITUBERCULAR agents , *PEDIATRICS , *ODDS ratio , *RESEARCH , *CASE-control method , *TUBERCULIN test , *CONFIDENCE intervals , *TUBERCULOSIS , *IMMUNOSUPPRESSION , *ADOLESCENCE , *CHILDREN - Abstract
Background In high-resource settings, the survival of children with immunocompromise (IC) has increased and immunosuppressive therapies are increasingly being used. This study aimed to determine the clinical characteristics, performance of diagnostic tools, and outcome of IC children with tuberculosis (TB) in Europe. Methods Multicenter, matched case-control study within the Pediatric Tuberculosis Network European Trials Group, capturing TB cases <18 years diagnosed 2000–2020. Results A total of 417 TB cases were included, comprising 139 children who are IC (human immunodeficiency virus, inborn errors of immunity, drug-induced immunosuppression, and other immunocompromising conditions) and 278 non-IC children as controls. Nonrespiratory TB was more frequent among cases than controls (32.4% vs 21.2%; P =.013). Patients with IC had an increased likelihood of presenting with severe disease (57.6% vs 38.5%; P <.001; odds ratio [95% confidence interval], 2.073 [1.37–3.13]). Children with IC had higher rates of false-negative tuberculin skin test (31.9% vs 6.0%; P <.001) and QuantiFERON-TB Gold assay (30.0% vs 7.3%; P <.001) results at diagnosis. Overall, the microbiological confirmation rate was similar in IC and non-IC cases (58.3% vs 49.3%; P =.083). Although the mortality in children with IC was <1%, the rate of long-term sequelae was significantly higher than in non-IC cases (14.8% vs 6.1%; P =.004). Conclusions Children with IC and TB in Europe have increased rates of nonrespiratory TB, severe disease, and long-term sequelae. Immune-based TB tests have poor sensitivity in those children. Future research should focus on developing improved immunological TB tests that perform better in patients with IC, and determining the reasons for the increased risk of long-term sequelae, with the aim to design preventive management strategies. [ABSTRACT FROM AUTHOR]
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- 2024
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34. Exploring the intersection of epigenetics, DNA repair, and immunology from studies of ICF syndrome, an inborn error of immunity.
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Motoko Unoki
- Subjects
DNA repair ,REGULATORY T cells ,DOUBLE-strand DNA breaks ,EPIGENETICS ,IMMUNOLOGY ,DNA mismatch repair ,RECESSIVE genes - Abstract
Immunodeficiency, centromeric instability, and facial anomalies (ICF) syndrome, a rare autosomal recessive disorder, manifests with hypoglobulinemia and chromosomal instability accompanied by DNA hypomethylation. Pathological variants in the DNMT3B, ZBTB24, CDCA7, or HELLS genes underlie its etiology. Activated lymphocytes from patients often display distinctive multiradial chromosomes fused via pericentromeric regions. Recent studies have provided deeper insights into how pathological variants in ICF-related proteins cause DNA hypomethylation and chromosome instability. However, the understanding of the molecular pathogenesis underlying immunodeficiency is still in its nascent stages. In the past half-decade, the roles of CDCA7, HELLS, and ZBTB24 in classical non-homologous end joining during double-strand DNA break repair and immunoglobulin class-switch recombination (CSR) have been unveiled. Nevertheless, given the decreased all classes of immunoglobulins in most patients, CSR deficiency alone cannot fully account for the immunodeficiency. The latest finding showing dysregulation of immunoglobulin signaling may provide a clue to understanding the immunodeficiency mechanism. While less common, a subgroup of patients exhibits T-cell abnormalities alongside B-cell anomalies, including reduced regulatory T-cells and increased effector memory T- and follicular helper T-cells. The dysregulation of immunoglobulin signaling in B-cells, the imbalance in T-cell subsets, and/or satellite RNA-mediated activation of innate immune response potentially explain autoimmune manifestations in a subset of patients. These findings emphasize the pivotal roles of ICF-related proteins in both B- and T-cell functions. ICF syndrome studies have illuminated many fundamental mechanisms. Further investigations will certainly continue to unveil additional mechanisms and their interplay. [ABSTRACT FROM AUTHOR]
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- 2024
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35. Metagenomic next-generation sequencing promotes diagnosis and treatment of Pneumocystis jirovecii pneumonia in non-HIV infected children: a retrospective study.
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Zhang, Zhenyu, Liu, Tingyan, Ming, Meixiu, Shen, Meili, Zhang, Yi, Chen, Hanlin, Chen, Weiming, Tao, Jinhao, Wang, Yixue, Liu, Jing, Zhou, Jihua, Lu, Guoping, and Yan, Gangfeng
- Subjects
PNEUMOCYSTIS pneumonia ,NUCLEOTIDE sequencing ,PEDIATRIC intensive care ,METAGENOMICS ,DIAGNOSIS ,PNEUMOCYSTIS jiroveci - Abstract
Background: Metagenomic next-generation sequencing (mNGS) excels in diagnosis of infection pathogens. We aimed to evaluate the performance of mNGS for the diagnosis of Pneumocystis jirovecii pneumonia (PJP) in non-HIV infected children. Methods: Totally 36 PJP children and 61 non-PJP children admitted to the pediatric intensive care unit from March 2018 to December 2021 were retrospectively enrolled. Clinical features of PJP children were summarized. 1,3-β-D glucan (BDG) test and bronchoalveolar lavage fluid (BALF) mNGS were used for evaluation of PJP diagnostic performance. Antimicrobial management modifications for PJP children after the mNGS results were also reviewed. Results: Pneumocystis jirovecii was detected in all PJP children by mNGS (36/36), and the sensitivity of mNGS was 100% (95% confidence interval [CI]: 90.26–100%). The sensitivity of BDG was 57.58% (95% CI: 39.22–74.52%). Of the 26 (72.2%) PJP patients with mixed infection, twenty-four (66.7%) were detected by BALF-mNGS. Thirteen patients (36.1%) had their antimicrobial management adjusted according to the mNGS results. Thirty-six PJP children included 17 (47.2%) primary immunodeficiency and 19 (52.8%) secondary immunodeficiency, of whom 19 (52.8%) survived and 17 (47.2%) died. Compared to survival subgroup, non-survival subgroup had a higher rate of primary immunodeficiency (64.7% vs. 31.6%, P = 0.047), younger age (7 months vs. 39 months, P = 0.011), lower body weight (8.0 kg vs. 12.0 kg, P = 0.022), and lower T lymphocyte counts. Conclusions: The mortality rate of PJP in immunosuppressed children without HIV infection is high and early diagnosis is challenging. BALF-mNGS could help identify PJP and guide clinical management. [ABSTRACT FROM AUTHOR]
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- 2024
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36. Economic impact of immunoglobulin replacement therapy in secondary immunodeficiency to hematological cancer: a single center observational study.
- Author
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del Campo Guerola, Luciana, García Sacristán, Ana Andrea, Portolés, Antonio, Jasso, Maricruz, Guerra-Galán, Teresa, de la Fuente-Munoz, Eduardo, Palacios-Ortega, María, Fernández-Arquero, Miguel, Cuesta-Mínguez, Cristina, Rodríguez-Sanz, Aránzazu, Peña-Cortijo, Ascensión, Polo, Marta, Mateo Morales, Marta, Anguita-Mandly, Eduardo, Benítez Jiménez, Teresa, Benavente Cuesta, Celina, and Sánchez-Ramón, Silvia
- Subjects
SEROTHERAPY ,HEMATOLOGIC malignancies ,EMERGENCY room visits ,ECONOMIC impact ,IMMUNODEFICIENCY - Abstract
This is the first report of the health economic benefits derived from preventing infections through Immunoglobulin Replacement Therapy (IgRT) in patients with secondary immunodeficiency due to hematological malignancies. We conducted a retrospective population-based cohort study using patient medical history and pharmacy data from the Hospital Clı'nico San Carlos for 21 patients between 2011 and 2020. The pharmacoeconomic impact of using prophylactic IgRT was assessed by comparing characteristics of the SID patients 1 year before and after initiating IgRT measured by direct medical and tangible indirect costs. Results indicate a marked reduction in hospitalization days following IgRT initiation, decreasing from an average of 13.9 to 6.1 days per patient, with the elimination of ICU admissions. While emergency department visits decreased significantly, the number of routine consultations remained unchanged. Notably, absenteeism from work dropped substantially. The financial analysis revealed significant reductions in medication use and fewer ancillary tests, resulting in considerable cost savings. Specifically, total expenditure dropped from €405,088.18 pre-IgRT to €295,804.42 post-IgRT--including the cost of IgRT itself at €156,309.60. Overall, the annual savings amounted to €109,283.84, validating the cost-effectiveness of IgRT in managing SID in patients with hematological cancers. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
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37. Decreased spermatogonial numbers in boys with severe haematological diseases.
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Lahtinen, Atte K., Funke, Miriam, Krallmann, Claudia, Wyrwoll, Margot J., Jarisch, Andrea, Yang, Yifan, Bjarnason, Ragnar, Romerius, Patrik, Sundin, Mikael, Norén‐Nyström, Ulrika, Langenskiöld, Cecilia, Cremers, Jann‐Frederik, Kliesch, Sabine, Stukenborg, Jan‐Bernd, Neuhaus, Nina, and Jahnukainen, Kirsi
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- *
HEMATOPOIETIC stem cell transplantation , *FANCONI'S anemia , *FERTILITY preservation , *APLASTIC anemia , *MYELOPROLIFERATIVE neoplasms , *MYELOFIBROSIS - Abstract
Summary: This study examines spermatogonial numbers in testicular samples from 43 prepubertal patients undergoing haematopoietic stem cell transplantation (HSCT). High‐dose chemotherapy and/or radiation during HSCT can impact spermatogenesis requiring fertility preservation. Results show that 49% of patients have decreased and 19% severely depleted spermatogonial pool prior to HSCT. Patients with Fanconi anaemia exhibit significantly reduced spermatogonial numbers. Patients with immunodeficiency or aplastic anaemia generally present within the normal range, while results in patients with myelodysplastic syndrome or myeloproliferative neoplasm vary. The study emphasizes the importance of assessing spermatogonial numbers in patients with severe haematological diseases for informed fertility preservation decisions. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
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38. Pneumococcal antibody response in children with recurrent respiratory tract infections: A descriptive study.
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Ceuppens, Falke, Meyts, Isabelle, Bossuyt, Xavier, and De Boeck, Kris
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ANTIBODY formation , *RESPIRATORY infections , *IMMUNOGLOBULIN G , *IMMUNOGLOBULIN A , *POLYSACCHARIDES - Abstract
The pneumococcal antibody response after vaccination with unconjugated pneumococcal vaccine can be evaluated as part of the diagnostic work-up of children with recurrent respiratory tract infections to detect an underlying polysaccharide antibody deficiency. Little is known about the prevalence of polysaccharide antibody deficiency in this population and its therapeutic consequences. This study aimed to investigate the prevalence of polysaccharide antibody deficiency in children with recurrent respiratory tract infections and to correlate polysaccharide responsiveness with clinical severity. In addition, we aimed to evaluate differences in the immunoglobulin (Ig)G2/IgG ratio, IgA level, and age in relation to the number of deficient serotype-specific antibody responses. Polysaccharide antibody titers for pneumococcal serotypes 8, 9N, and 15B; clinical characteristics; and immunoglobulin levels of 103 children with recurrent respiratory tract infections were retrospectively assessed. American Academy of Allergy, Asthma, and Immunology guidelines were used for the interpretation of the polysaccharide antibody response. Overall, 28 children (27.2 %) were diagnosed with polysaccharide antibody deficiency. No correlation was found between the number of deficient serotype-specific antibody responses and clinical severity. The study participants with a normal response to all three serotypes had a higher IgG2/IgG ratio than those with one or more deficient responses (p < 0.003). No significant correlation between IgA levels and polysaccharide responsiveness was found. The median age of children with normal polysaccharide responsiveness for the three tested serotypes was higher than that of children with a deficient response to one or more serotypes (p < 0.0025). For a large group of children (18.4 %) with recurrent respiratory tract infections, an underlying mechanism for their susceptibility was defined thanks to diagnostic unconjugated pneumococcal polysaccharide vaccination. Further research is needed to formulate age-specific normal values for polysaccharide responsiveness and to investigate the usefulness of the IgG2/IgG ratio in determining the need for diagnostic unconjugated pneumococcal polysaccharide vaccination. [ABSTRACT FROM AUTHOR]
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- 2024
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39. Clinical, immunological, and molecular findings in two patients with MHC class I deficiency and post‐transplant outcome.
- Author
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Ramalingam, Thulasi Raman, Vaidhyanathan, Lakshman, NK, Harsha Rasheed, Uppuluri, Ramya, and Raj, Revathi
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KILLER cells , *CYTOTOXIC T cells , *SEVERE combined immunodeficiency , *GRANULOMATOSIS with polyangiitis , *WHEEZE - Abstract
This article explores the clinical, immunological, and molecular aspects of MHC class I deficiency, a rare immunodeficiency disorder characterized by the absence or reduced expression of MHC class I molecules. These molecules play a crucial role in presenting peptides from intracellular pathogens to CD8+ T cells, which eliminate infected cells. The article presents two cases of children with MHC class I deficiency who underwent hematopoietic stem cell transplantation (HSCT) and discusses their outcomes after the transplant. MHC class I deficiency can lead to susceptibility to infections and malignancies, and its symptoms can vary from mild to severe. The role of transplantation in treating this condition is not well understood, as immune reconstitution is limited due to poor thymic function. Further research is necessary to gain a better understanding of MHC class I deficiency and its treatment options. [Extracted from the article]
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- 2024
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40. Inborn errors of immunity and protozoa.
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de Gouveia‐Pereira Pimentel, Mariana, Aranda, Carolina Sanchez, Puccini, Pedro Fiorini, da Silva, Celso José Mendanha, Lopes, Leticia Helena Caldas, Villalba‐Alemán, Evaristo, Gava, Ricardo, de Souza, Thiago Kury Moreno, Teixeira, Lara Novaes, Salvador, Luiza Schimd, Solé, Dirceu, Tolezano, José Eduardo, and Teixeira, Marta Maria Geraldes
- Subjects
- *
IMMUNITY , *PROTOZOA , *NEGLECTED diseases , *IMMUNOPHENOTYPING , *CUTANEOUS leishmaniasis - Abstract
This article explores the field of immunology and its connection to inborn errors of immunity (IEI) and protozoa. IEI refers to a range of diseases caused by genetic defects that affect the immune system, making individuals more susceptible to infections. The article emphasizes the need to understand the characteristics of the disease and the infective agent to identify compromised immune mechanisms. It also discusses the global health challenges posed by neglected tropical diseases (NTDs) and their impact on marginalized populations. The article presents a case study of a child with an inborn error of immunity and unusual infections, highlighting the need for innovative diagnostic assays and treatment protocols. The case underscores the importance of collaboration among immunologists in Brazil to enhance diagnostic capabilities and improve patient care for uncommon infections. [Extracted from the article]
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- 2024
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41. BCL11B‐Related Dystonia: Further Evidence of an Emerging Cause of Childhood‐Onset Generalized Dystonia.
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Garone, Giacomo, Capuano, Alessandro, Amodio, Donato, Nicita, Francesco, Travaglini, Lorena, Graziola, Federica, De Benedictis, Alessandro, Frascarelli, Flaminia, Parisi, Pasquale, Pizzi, Simone, Tartaglia, Marco, Marras, Carlo Efisio, and Niceta, Marcello
- Subjects
- *
MOVEMENT disorders , *DYSTONIA , *MEDIUM spiny neurons , *CYCLIC adenylic acid , *KILLER cells , *DEEP brain stimulation - Abstract
This article discusses BCL11B-related dystonia, a genetic disorder that causes childhood-onset generalized dystonia and intellectual disability. The BCL11B gene is involved in the development of various systems in the body. Mutations in this gene have been linked to a syndrome characterized by intellectual disability, facial abnormalities, and T cell abnormalities. Recent case reports suggest that a complex movement disorder, including spasticity and dystonia, can also be part of this syndrome. The article presents a case study of a young girl with BCL11B-related dystonia, providing information about her symptoms, genetic testing, and treatment. The study suggests that BCL11B should be considered in the diagnosis of genetic causes of generalized dystonia with childhood onset and intellectual disability. The research was conducted with the consent of the patient's guardians and was funded by the Italian Ministry of Health. [Extracted from the article]
- Published
- 2024
- Full Text
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42. Proliferative Retinopathy in Non-human Immunodeficiency Virus CMV Retinitis.
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Puri, Prabhav and Kumar, Vinod
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HIV , *DIABETIC retinopathy , *PARS plana , *VASCULAR endothelial growth factors , *VISUAL acuity , *IMMUNODEFICIENCY - Abstract
To report a case series of human immunodeficiency virus (HIV)-negative patients with healed cytomegalovirus retinitis presenting with proliferative retinopathy (in the form of neovascularisation elsewhere). Retrospective case series. Multimodal imaging was performed at each follow-up visit. Three patients with non-HIV immune dysfunction were followed up after healing of CMV retinitis. All three developed neovascularisation. Patient 1 after 4 months presented with vitreous haemorrhage for which pars plana vitrectomy was performed. Patient 2 developed neovascularization at disc and neovascularisation elsewhere 4 months after resolution, and patient 3 despite being affected by bilateral CMV retinitis, presented with unilateral neovascularization at 14 month after resolution of retinitis. Increased incidence of this rare entity could be attributed to partial immune dysfunction in non-HIV patients, limited area of retinitis with a more aggressive occlusive vasculitis. Extensive occlusion with more area of viable retina for angiogenic factor production explains this phenomena. It emphasizes the need for continued follow-up even after healing and to differentiate it from reactivation of retinitis and immune recovery uveitis.Abbreviation: CMV: cytomegalovirus; HIV: human immunodeficiency virus; BCVA: best corrected visual acuity [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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43. Immunodeficiency-Related Vaccine-Derived Poliovirus (iVDPV) Excretion in an Infant with Severe Combined Immune Deficiency with Spillover to a Parent.
- Author
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Mohanty, Madhu Chhanda, Govindaraj, Geeta, Ahmad, Mohammad, Varose, Swapnil Y., Tatkare, Manogat, Shete, Anita, Yadav, Savita, Joshi, Yash, Yadav, Pragya, Sharma, Deepa, Kumar, Arun, Verma, Harish, Patil, Ankita P., Edavazhipurath, Athulya, Dhanasooraj, Dhananjayan, Othayoth Kandy, Sheena, Puthenpurayil, Jayakrishnan Machinary, Chakyar, Krishnan, Melarcode Ramanan, Kesavan, and Madkaikar, Manisha
- Subjects
HEMATOPOIETIC stem cells ,STEM cell transplantation ,PRIMARY immunodeficiency diseases ,MISSENSE mutation ,IMMUNODEFICIENCY ,ACUTE flaccid paralysis - Abstract
In order to maintain the polio eradication status, it has become evident that the surveillance of cases with acute flaccid paralysis and of environmental samples must be urgently supplemented with the surveillance of poliovirus excretions among individuals with inborn errors of immunity (IEI). All children with IEI were screened for the excretion of poliovirus during a collaborative study conducted by the ICMR-National Institute of Virology, Mumbai Unit, ICMR-National Institute of Immunohaematology, and World Health Organization, India. A seven-month -old male baby who presented with persistent pneumonia and lymphopenia was found to have severe combined immune deficiency (SCID) due to a missense variant in the RAG1 gene. He had received OPV at birth and at 20 weeks. Four stool samples collected at 4 weekly intervals yielded iVDPV type 1. The child's father, an asymptomatic 32-year-old male, was also found to be excreting iVDPV. A haploidentical hematopoietic stem cell transplant was performed, but the child succumbed due to severe myocarditis and pneumonia three weeks later. We report a rare case of transmission of iVDPV from an individual with IEI to a healthy household contact, demonstrating the threat of the spread of iVDPV from persons with IEI and the necessity to develop effective antivirals. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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44. Orodental health status of patients with inborn errors of immunity.
- Author
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Chaiboonyarak, Thitaya, Chantarangsu, Soranun, Gavila, Patcharaporn, Lao‐Araya, Mongkol, Suratannon, Narissara, and Porntaveetus, Thantrira
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PRIMARY immunodeficiency diseases ,RISK assessment ,MEDICAL care use ,ANTIBIOTICS ,RESEARCH funding ,LOGISTIC regression analysis ,GINGIVITIS ,ORAL hygiene ,CANKER sores ,DENTAL calculus ,AGE distribution ,ORAL diseases ,DENTAL pathology ,ECONOMIC impact ,DENTAL caries ,CHILDREN'S dental care ,CANDIDIASIS ,DEVELOPMENTAL defects of enamel ,ORAL health ,PERIODONTITIS ,DISEASE risk factors ,DISEASE complications - Abstract
Background: Various orodental problems affect patients with inborn errors of immunity (IEI), but there are limited studies on these issues. Aim: To study orodental status and its confounding factors in patients with IEI. Design: Caries, enamel defects, gingival, and soft tissue conditions were examined. Data on patient characteristics, dental hygiene habits, dental attendance, and household income were collected. Statistical analysis and logistic regression were performed. Results: Forty‐five participants with a mean age of 9.20 ± 6.41 years were included. Almost all participants had gingivitis (42 of 45; 93.3%), whereas a small number had periodontitis (five of 45; 11.1%). Calculus was found in 33 (73.3%) and caries in 30 (66.7%). Mucosal ulcers, enamel defects, and candidiasis were observed in 23 of 45 (51.1%), 16 of 43 (37.2%), and six of 43 (14.0%), respectively. Chances of having caries, moderate‐to‐severe gingivitis, periodontitis, calculus, and ulcers increased with age. Taking antibiotics in the last two months increased the risk of caries by five times. Lower income increased the risk of calculus deposit by nine times. Conclusion: Gingivitis, calculus, caries, and mucosal ulcers were the most common orodental findings in patients with IEI. Antibiotics increased the risk of caries, and low‐income children had higher calculus accumulation. [ABSTRACT FROM AUTHOR]
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- 2024
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45. Deficiency of Adenosine Deaminase 2
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Çağrı Coşkun and Şule Ünal
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deficiency of adenosine deaminase 2 ,ada2 gene ,pure red cell aplasia ,autoinflammatory disease ,immunodeficiency ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Adenosine deaminase 2 (ADA2) deficiency is an autosomal recessively inherited autoinflammatory disorder caused by loss-of-function mutations in the ADA2 gene. Although the pathogenesis involves the triggering of a proinflammatory cascade due to increased production of inflammatory cytokines such as tumor necrosis factor (TNF)-α and dysregulation of neutrophil extracellular trap formation resulting from an excess accumulation of extracellular adenosine, the pathogenetic mechanism still needs further clarification due to the broad clinical spectrum. In addition to the initially described vasculitis-related symptoms, hematological, immunological, and autoinflammatory symptoms are now well recognized. The diagnosis is made by demonstration of pathogenic variants of ADA2 with biallelic loss of function and identification of low plasma ADA2 catalytic activity. Currently, TNF-α inhibitors are the treatment of choice for controlling vasculitis manifestations and preventing strokes. However, in patients presenting with severe hematologic findings, TNF-α inhibitors are not the treatment of choice and hematopoietic stem cell transplantation has been shown to be successful in selected cases. Recombinant ADA2 protein and gene therapy are promising treatment modalities for the future. In conclusion, ADA2 deficiency has a broad phenotype and should be considered in the differential diagnosis of different clinical situations. In this review, we summarize the disease manifestations of ADA2 deficiency and available treatment options.
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- 2024
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46. A case report of empyema caused by Enterococcus gallinarum
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Min Liu, Jixiang Liu, Juanjuan Wu, Shuang Liu, Lu Sun, Fajiu Li, and Chenghong Li
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Enterococcus gallinarum ,Empyema ,Immunodeficiency ,Thoracoscopy ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Enterococcus gallinarum is an infrequently intestinal symbiotic pathogen associated with nosocomial infection in immunocompromised individuals. To date, rare cases of pulmonary infection attributable to Enterococcus gallinarum were reported. Herein, we presented the first case of empyema resulting from Enterococcus gallinarum infection. Case presentation An 81-year-old male presented with fever and dyspnea upon admission. Chest CT scan and thoracic ultrasonography confirmed the presence of right pleural effusion. Thoracoscopy revealed extensive adhesion, purulent fluid, and necrotic materials within the thoracic cavity. Enterococcus gallinarum was identified through pleural effusion culture. The patient underwent an intrathoracic injection of urokinase along with thoracic drainage. Following surgery, He took oral linezolid for over one month. Undergoing comprehensive treatment, the patient exhibited favorable recovery. Conclusions We reported the first case of empyema due to Enterococcus gallinarum infection. It should be suspected in patients with impaired immune function and invasive therapies, without responding to conventional anti-infectious treatment.
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- 2024
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47. Presumptive Cytomegalovirus Retinitis as a Complication of Dyskeratosis Congenita: A Case Report
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Yuxi Du and Yalong Dang
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dyskeratosis congenita ,dkc1 gene ,cytomegalovirus ,retinitis ,immunodeficiency ,Ophthalmology ,RE1-994 - Abstract
Introduction: Dyskeratosis congenita is a rare genetic disorder characterized by abnormalities of the skin, nails, and oral mucosa. Retinal involvement in this condition is uncommon. Here, we present a case of a young male patient diagnosed with presumptive cytomegalovirus retinitis, ultimately found to be concomitant with dyskeratosis congenita. Case Presentation: A non-HIV-infected young male with recurrent infections, including aspergillus pneumonia and pneumocystis pneumonia, presented with presumptive cytomegalovirus retinitis in both eyes. Systemic manifestations included cutaneous hyperpigmentation, nail dystrophy, and oral mucosal leukoplakia. Genetic testing revealed a mutation in the DKC1 gene. The final diagnosis was dyskeratosis congenita complicated by presumptive cytomegalovirus retinitis. Conclusion: Cytomegalovirus retinitis can serve as an ocular complication of dyskeratosis congenita. When a patient presents with cytomegalovirus retinitis, a comprehensive systematic examination should be conducted as it indicates severe immunodeficiency.
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- 2024
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48. Metagenomic next-generation sequencing promotes diagnosis and treatment of Pneumocystis jirovecii pneumonia in non-HIV infected children: a retrospective study
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Zhenyu Zhang, Tingyan Liu, Meixiu Ming, Meili Shen, Yi Zhang, Hanlin Chen, Weiming Chen, Jinhao Tao, Yixue Wang, Jing Liu, Jihua Zhou, Guoping Lu, and Gangfeng Yan
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Pediatric intensive care unit ,Bronchoalveolar lavage fluid (BALF) ,Immunodeficiency ,Clinical characteristics ,Prognosis ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background Metagenomic next-generation sequencing (mNGS) excels in diagnosis of infection pathogens. We aimed to evaluate the performance of mNGS for the diagnosis of Pneumocystis jirovecii pneumonia (PJP) in non-HIV infected children. Methods Totally 36 PJP children and 61 non-PJP children admitted to the pediatric intensive care unit from March 2018 to December 2021 were retrospectively enrolled. Clinical features of PJP children were summarized. 1,3-β-D glucan (BDG) test and bronchoalveolar lavage fluid (BALF) mNGS were used for evaluation of PJP diagnostic performance. Antimicrobial management modifications for PJP children after the mNGS results were also reviewed. Results Pneumocystis jirovecii was detected in all PJP children by mNGS (36/36), and the sensitivity of mNGS was 100% (95% confidence interval [CI]: 90.26–100%). The sensitivity of BDG was 57.58% (95% CI: 39.22–74.52%). Of the 26 (72.2%) PJP patients with mixed infection, twenty-four (66.7%) were detected by BALF-mNGS. Thirteen patients (36.1%) had their antimicrobial management adjusted according to the mNGS results. Thirty-six PJP children included 17 (47.2%) primary immunodeficiency and 19 (52.8%) secondary immunodeficiency, of whom 19 (52.8%) survived and 17 (47.2%) died. Compared to survival subgroup, non-survival subgroup had a higher rate of primary immunodeficiency (64.7% vs. 31.6%, P = 0.047), younger age (7 months vs. 39 months, P = 0.011), lower body weight (8.0 kg vs. 12.0 kg, P = 0.022), and lower T lymphocyte counts. Conclusions The mortality rate of PJP in immunosuppressed children without HIV infection is high and early diagnosis is challenging. BALF-mNGS could help identify PJP and guide clinical management.
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- 2024
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49. Wikijournal preprints/impact of xenogenic mesenchymal stem cells secretome on a humoral component of the immune system
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Moskalov, Vitalii, Koshova, Olena, Ali, Sabina, Filimonova, Nataliia, and Tishchenko, Irina
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- 2023
50. JAKs and STATs from a Clinical Perspective: Loss-of-Function Mutations, Gain-of-Function Mutations, and Their Multidimensional Consequences.
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Ott, Nils, Faletti, Laura, Andreani, Virginia, Grimbacher, Bodo, and Heeg, Maximilian
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Autoimmunity ,Autoinflammation ,Clinical phenotype ,Gain-of-function mutations ,Immunodeficiency ,Inborn errors of immunity ,JAK/STAT signaling pathway ,JAK1 ,JAK3 ,Loss-of-function mutations ,STAT1 ,STAT3 ,STAT6 ,Humans ,Gain of Function Mutation ,Janus Kinases ,Signal Transduction ,Cytokines ,STAT Transcription Factors - Abstract
The JAK/STAT signaling pathway plays a key role in cytokine signaling and is involved in development, immunity, and tumorigenesis for nearly any cell. At first glance, the JAK/STAT signaling pathway appears to be straightforward. However, on closer examination, the factors influencing the JAK/STAT signaling activity, such as cytokine diversity, receptor profile, overlapping JAK and STAT specificity among non-redundant functions of the JAK/STAT complexes, positive regulators (e.g., cooperating transcription factors), and negative regulators (e.g., SOCS, PIAS, PTP), demonstrate the complexity of the pathways architecture, which can be quickly disturbed by mutations. The JAK/STAT signaling pathway has been, and still is, subject of basic research and offers an enormous potential for the development of new methods of personalized medicine and thus the translation of basic molecular research into clinical practice beyond the use of JAK inhibitors. Gain-of-function and loss-of-function mutations in the three immunologically particularly relevant signal transducers STAT1, STAT3, and STAT6 as well as JAK1 and JAK3 present themselves through individual phenotypic clinical pictures. The established, traditional paradigm of loss-of-function mutations leading to immunodeficiency and gain-of-function mutation leading to autoimmunity breaks down and a more differentiated picture of disease patterns evolve. This review is intended to provide an overview of these specific syndromes from a clinical perspective and to summarize current findings on pathomechanism, symptoms, immunological features, and therapeutic options of STAT1, STAT3, STAT6, JAK1, and JAK3 loss-of-function and gain-of-function diseases.
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- 2023
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