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An inexpensive at-home quantitative analysis activity was developed for the determination of glucose in unknown samples using paper-based microfluidic devices. All of the materials and reagents for the activity fit in a small kit that was mailed to students. The only items students needed to supply were water and a smartphone. Microgram quantities of glucose were dried down in microcentrifuge tubes and included in the kit so that students could prepare external standards. Student results distinguished between two different concentrations of glucose that served as unknown samples. The combination of paper-based devices and dried-down microgram quantities of reagents provides a foundation for the development of other at-home or in-person experiments.

The synthesis and characterization of RuC7, a complex in which a heme is covalently attached to a [Ru(bpy)3](super 2+) complex through a -(CH2)(sub 7)- linker is reported. Strong EPR signals attributed to tyrosine and tryptophan radicals are recorded after RuC7MbM(super 3+) (M = Fe, Mn) was flash-quenched/frozen.

Close through-space proximity of the dye to the dendrimer and the restricted motion of the encapsulated dye are revealed. Cytotoxicity assays with human breast cancer cells showed a significant reduction of cell viability, demonstrating that 10HCPT retains activity upon encapsulation.

The study explains how CYP119 hydroxylates lauric acid in a reaction supported by putidaredoxin and putidaredoxin reductase. This helps to engineer the mutagenesis of the complementarity of the protein-protein interface.

A study was conducted to examine the effects of the systematic modification of (Ru(trpy)(L)(OH2))2+ on the electrocatalytic rate of benzyl alcohol oxidation. The (Ru(trpy)(L)(OH2))2+ was represented as; L was equal to 3,6-bis(pyrid-2-yl)pyridazine (H2dppi), 3,6-bis(6-methylpyrid-2-yl)pyridazine (me2dppi), and 3,6-bis(6-chloropyrid-2-yl)pyridazine (Cl2dppi). The Perkin-Elmer Lamda 11 spectrometer was utilized to examine the complexes. Results revealed that the steric effects, which resulted from the incorporation of chloro or methyl group to a ligand, might have encouraged a dependence of the oxidation rates.

A novel class of heterobimetallic dendrimers were prepared by utilizing a convergent approach that was based on the oxidative addition chemistry of platinum(II). The resulting heterobimetallic dendrimers exhibited ferrocenyl moieties that were arranged concentrically around the periphery of the molecule. On the other hand, nuclear magnetic resonance analysis inficated the presence of platinum(IV) bipyridyl units of the complex near the central atom.

The binding of nitrosyl hydride (HNO) to deoxymyoglobin is rapid and essentially irreversible. This suggests that the biological activity of nitroxyl may be mediated by its reactivity with ferrous heme proteins such as myoglobin and hemoglobin.

A resonance Raman (RR) and X-ray absorption characterization of Mb-HNO, which yields new information on bonding within this species, is discussed. The results provided definitive evidence for the structure of Mb-HNO, which supports the structure deduced on the basis of NMR experiments and is the first structural characterization of an HNO bound to a heme protein.

The synthesis of oligodeoxynucleotide-PEG is described. A 5'- terminal ferrocene phosphoramidite was selected as redox reporter group for these experiments.

Reagent pencils allow for solvent-free deposition of reagents onto paper-based microfluidic devices. The pencils are portable, easy to use, extend the shelf-life of reagents, and offer a platform for customizing diagnostic devices at the point of care. In this work, reagent pencils were characterized by measuring the wear resistance of pencil cores made from polyethylene glycols (PEGs) with different molecular weights and incorporating various concentrations of three different reagents using a standard pin abrasion test, as well as by measuring the efficiency of reagent delivery from the pencils to the test zones of paper-based microfluidic devices using absorption spectroscopy and digital image colorimetry. The molecular weight of the PEG, concentration of the reagent, and the molecular weight of the reagent were all found to have an inverse correlation with the wear of the pencil cores, but the amount of reagent delivered to the test zone of a device correlated most strongly with the concentration of the reagent in the pencil core. Up to 49% of the total reagent deposited on a device with a pencil was released into the test zone, compared to 58% for reagents deposited from a solution. The results suggest that reagent pencils can be prepared for a variety of reagents using PEGs with molecular weights in the range of 2000 to 6000 g/mol. [ABSTRACT FROM AUTHOR]

[reaction: see text] A facile procedure for the incorporation of N-methyl phenothiazine as the terminal nucleoside in oligodeoxynucleotides is reported. The phenothiazine nucleoside analogue is synthesized and then incorporated into DNA using an automated DNA solid-phase synthesizer. Phenothiazine-labeled oligodeoxynucleotides form stable B-form duplexes with higher melting temperatures compared to unlabeled DNA duplexes.