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3 results on '"INNPRONTA"'

1. Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS

Author

Mercè Mercader-Martí, Anna Vallverdú-Queralt, Rosa M. Lamuela-Raventós, Nuria Boix, Gemma Sasot, Juan M. Llobet, Alexander Medina-Remón, Jesús Gómez-Catalán, Ester Piqué, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III (ISC), GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, Nutrition and Food Science Department, XaRTA, Pharmacy School, Miguel Torres S.A., Institute of Nutrition and Food Safety (INSA) of the University of Barcelona [FRI2012], INNPRONTA, CICYT [AGL2010-22319-C03, AGL2013-49083-C3-1-R], Instituto de Salud Carlos III, ISCIII (ciberobn) from the Spanish Ministry of Science and Innovation Ministerio de Ciencia e Innovacion (MICINN), Quality Group from Generalitat de Catalunya Generalitat de Catalunya (GC) [2014 SGR 773, SGR 566], Foundation Alfonso Martin Escudero for carrying out research in foreign countries, 'Juan de la Cierva' postdoctoral program from Ministerio de Economia y Competitividad [JCI-2012-13463], Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Instituto de Salud Carlos III [Madrid] (ISC), and Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
Spectrometry, Mass, Electrospray Ionization, Identification, Electrospray, animal structures, Zebrafish (ZF), 030309 nutrition & dietetics, [SDV]Life Sciences [q-bio], Wine, Mass spectrometry, 01 natural sciences, High-performance liquid chromatography, Mass Spectrometry, Analytical Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Phenols, Animals, Zebrafish, Chromatography, High Pressure Liquid, 0303 health sciences, Orbitrap-MS, Chromatography, biology, 010401 analytical chemistry, Polyphenols, food and beverages, Red wine extract, General Medicine, biology.organism_classification, 3. Good health, 0104 chemical sciences, chemistry, Polyphenol, Anthocyanin, embryonic structures, Ion trap, Chromatography, Liquid, Food Science
Abstract

The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS(2) and MS(3) modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry.

Published
2015
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2. Identification of phenolic metabolites in human urine after the intake of a functional food made from grape extract by a high resolution LTQ-Orbitrap-MS approach

Author

Gemma Sasot, Miriam Martínez-Huélamo, Rosa M. Lamuela-Raventós, Mercè Mercader-Martí, Ramon Estruch, Anna Vallverdú-Queralt, Nutrition, Food Science and Gastronomy Department, School of Pharmacy and Food Science, University of Barcelona, Biomedical Research Center in Red-Physiopathology of Obesity and Nutrition (CIBERobn), INSA-UB, Nutrition and Food Safety Research Institute, Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Miguel Torres S.A., Department of Internal Medicine, Hospital Clinic, Institute of Biomedical Investigation August Pi i Sunyer (IDIBAPS), INNPRONTA Project [IPT-20111008], CICY [AGL2013-49083-C3-1-R, AGL2016-79113-R], Instituto de Salud Carlos III, ISCIII (CIBEROBN) from Spanish Ministrerio de Economia y Competitividad (MEC) (AEI/FEDER, UE), Generalitat de Catalunya (GC) [2014 SGR 773], Alfonso Martin Escudero Foundation [EA 1517], Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
Adult, Male, 0301 basic medicine, Spectrometry, Mass, Electrospray Ionization, Resolution (mass spectrometry), polyphenol metabolites, Electrospray ionization, mass spectrum analysis, grape pomace, Urine, Mass spectrometry, 01 natural sciences, Young Adult, 03 medical and health sciences, polyphénol, Flavonols, Phenols, Functional food, Functional Food, Reference Values, human urine, spectrométrie de masse, Humans, Vitis, Prospective Studies, Chromatography, High Pressure Liquid, LTQ-Orbitrap, polyphenols, chemistry.chemical_classification, marc de raisin, Cross-Over Studies, 030109 nutrition & dietetics, Chromatography, Plant Extracts, 010401 analytical chemistry, Pomace, 0104 chemical sciences, chemistry, Polyphenol, high resolution mass spectrometry, identification, Female, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract

Grape pomace (GP) is known to be a rich source of polyphenols with biological activity which may be used as functional ingredients for the development of new health-promoting products. Numerous studies have reported that bioactive compounds may act through multiple mechanisms. In order to verify the oral absorption and metabolism of grape polyphenols, we performed a prospective, randomised and cross-over acute study in 12 volunteers with two interventions: 500 mL of a functional beverage enriched with 200 mL of GP and 500 mL of a control beverage without GP. In this work, liquid chromatography coupled with an electrospray ionization hybrid linear ion trap quadrupole-Orbitrap-mass spectrometry (LC/ESI-LTQ-Orbitrap-MS) technique has been used to accurately identify phenolics in GP and human urine. In GP, 41 phenolic compounds were identified mainly procyanidins, phenolic acids and flavonols, and in human urine over 70 metabolites of phenolic compounds including microbiota metabolites, glucuronides and sulfate derivatives were detected. Overall, high resolution mass spectrometry (HR-MS) enhances the identification of a large variety of polyphenols and their metabolites with great mass accuracies for all molecular ions.

Published
2017
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3. MicroRNA deregulation in triple negative breast cancer reveals a role of miR-498 in regulating BRCA1 expression

Author

José Ignacio Arias, Kira Yanowsky, Eduardo Andrés-León, Primitiva Menéndez, Ana Llaneza-Folgueras, Beatriz Martinez-Delgado, María Teresa Vargas, Ricardo González-Cámpora, Nerea Matamala, Rebeca Miñambres, Javier Benitez, Ministerio de Economía y Competitividad (España), [Matamala,N, Yanowsky,K, Benítez,J] Human Cancer Genetics Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. [Vargas,MT, González-Cámpora,R] Pathology Service, Hospital Virgen de la Macarena, Sevilla, Spain. [Arias,JI, Menéndez,P]Surgery Service.Pathology Service, Hospital Monte Naranco, Oviedo, Spain.[Andrés-León,E] Bioinformatics Unit, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. Bioinformatics Unit, Instituto de Parasitología y Biomedicina 'López Neyra', Consejo Superior de Investigaciones Científicas (IPBLN-CSIC), Granada, Spain. [Llaneza-Folgueras,A] General Surgery Service, Hospital Universitario Central de Asturias, Oviedo, España. [Miñambres,R] Projects Unit, Sistemas Genómicos, Valencia, Spain. [Martínez-Delgado,B] Molecular Genetics Unit, Research Institute of Rare Diseases (IIER), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.[Benítez.J] Network on Rare Diseases (CIBERER), Madrid, Spain. [Matamala,N] Molecular Genetics Unit, Research Institute of Rare Diseases (IIER), Instituto de Salud Carlos III (ISCIII), Madrid, Spain. [Vargas,MT] Hematology Service, Hospital Virgen de la Macarena, Sevilla, Spain., and The Spanish Ministry of Economy and Competitiveness (INNPRONTA LIFE project) and FIS PI11/01059 (BM-D).

Subjects
0301 basic medicine, Pathology, Regulación hacia abajo, Apoptosis, Triple Negative Breast Neoplasms, Anatomy::Body Regions::Breast [Medical Subject Headings], Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], 0302 clinical medicine, Anatomy::Cells::Cells, Cultured::Cell Line [Medical Subject Headings], Oligonucleótidos, Neoplasias mamarias animales, Organisms::Eukaryota::Animals [Medical Subject Headings], Medicine, Luciferasas, Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Paraffin [Medical Subject Headings], skin and connective tissue diseases, Triple-negative breast cancer, Cells, Cultured, Regulation of gene expression, microRNA, BRCA1 Protein, Línea celular, Mama, Gene Expression Regulation, Neoplastic, Diseases::Neoplasms::Neoplasms by Site::Breast Neoplasms::Triple Negative Breast Neoplasms [Medical Subject Headings], Oncology, 030220 oncology & carcinogenesis, Female, DNA microarray, Research Paper, medicine.medical_specialty, Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleotides::Polynucleotides [Medical Subject Headings], Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Luciferases [Medical Subject Headings], 03 medical and health sciences, Breast cancer, Downregulation and upregulation, Biomarkers, Tumor, Humans, Cell Proliferation, Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Down-Regulation [Medical Subject Headings], business.industry, Cell growth, Parafina, Diseases::Neoplasms::Neoplasms by Site::Mammary Neoplasms, Animal [Medical Subject Headings], Neoplasias de la mama triple negativas, medicine.disease, BRCA1, Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Antisense Elements (Genetics)::RNA, Antisense::MicroRNAs [Medical Subject Headings], MicroRNAs, 030104 developmental biology, triple negative breast cancer, Case-Control Studies, Cancer research, business
Abstract

Emerging evidence suggests that BRCA1 pathway contributes to the behavior of sporadic triple negative breast cancer (TNBC), but little is known about the mechanisms underlying this association. Considering the central role that microRNAs (miRNAs) play in gene expression regulation, the aim of this study was to identify miRNAs specifically deregulated in TNBC and investigate their involvement in BRCA1 regulation. Using locked nucleic acid (LNA)-based microarrays, expression levels of 1919 miRNAs were measured in paraffin-embedded tissues from 122 breast tumors and 11 healthy breast tissue samples. Differential miRNA expression was explored among the main subtypes of breast cancer, and 105 miRNAs were identified as specific for triple negative tumors. In silico prediction revealed that miR-498 and miR-187-5p target BRCA1, and these results were confirmed by luciferase reporter assay. While miR-187-5p was found overexpressed in a luminal B cell line, miR-498 was highly expressed in a triple negative cell line, Hs578T, and its expression was negatively correlated with the levels of BRCA1. We functionally demonstrated that miR-498 inhibits BRCA1 in breast cancer cell lines, and showed that inhibition of miR-498 led to reduced proliferation in the triple negative cell line Hs578T. Our results indicate that miR-498 regulates BRCA1 expression in breast cancer and its overexpression could contribute to the pathogenesis of sporadic TNBC via BRCA1 downregulation. We thank all members of the Human Cancer Genetics Programme of the Spanish National Cancer Research Centre for all their support. We want to particularly acknowledge the patients, the INCLIVA BioBank (PT13/0010/0004) integrated in the Spanish National Biobanks Network, and Pablo Isidro Marrón, coordinator of Biobanco del Principado de Asturias, for their collaboration. This work has been funded by grants from the Spanish Ministry of Economy and Competitiveness (INNPRONTA LIFE project) and FIS PI11/01059 (BM-D). Sí

Published
2016

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