164 results on '"Huynh, Bich-Tram"'
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2. Assessing respiratory epidemic potential in French hospitals through collection of close contact data (April–June 2020)
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Shirreff, George, Huynh, Bich-Tram, Duval, Audrey, Pereira, Lara Cristina, Annane, Djillali, Dinh, Aurélien, Lambotte, Olivier, Bulifon, Sophie, Guichardon, Magali, Beaune, Sebastien, Toubiana, Julie, Kermorvant-Duchemin, Elsa, Chéron, Gerard, Cordel, Hugues, Argaud, Laurent, Douplat, Marion, Abraham, Paul, Tazarourte, Karim, Martin-Gaujard, Géraldine, Vanhems, Philippe, Hilliquin, Delphine, Nguyen, Duc, Chelius, Guillaume, Fraboulet, Antoine, Temime, Laura, Opatowski, Lulla, and Guillemot, Didier
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- 2024
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3. Incidence and risk factors of neonatal bacterial infections: a community-based cohort from Madagascar (2018–2021)
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Devred, Ines, Rambliere, Lison, Herindrainy, Perlinot, Andriamarohasina, Lovarivelo, Harimanana, Aina, Randrianirina, Frederique, Ratsima, Elisoa Hariniaina, Hivernaud, Delphine, Kermorvant-Duchemin, Elsa, Andrianirina, Zafitsara Zo, Abdou, Armya Youssouf, Delarocque-Astagneau, Elisabeth, Guillemot, Didier, Crucitti, Tania, Collard, Jean-Marc, and Huynh, Bich-Tram
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- 2023
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4. Asymptomatic infections with Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis among women in low- and middle-income countries: A systematic review and meta-analysis
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Fortas, Camille, primary, Delarocque-Astagneau, Elisabeth, additional, Randremanana, Rindra Vatosoa, additional, Crucitti, Tania, additional, and Huynh, Bich-Tram, additional
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- 2024
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5. Novel point-of-care cytokine biomarker lateral flow test for the screening for sexually transmitted infections and bacterial vaginosis: study protocol of a multicentre multidisciplinary prospective observational clinical study to evaluate the performance and feasibility of the Genital InFlammation Test (GIFT)
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Ramboarina, Stephanie, primary, Crucitti, Tania, additional, Gill, Katherine, additional, Bekker, Linda Gail, additional, Harding-Esch, Emma Michele, additional, van de Wijgert, Janneke H H M, additional, Huynh, Bich-Tram, additional, Fortas, Camille, additional, Harimanana, Aina, additional, Mayouya Gamana, Théodora, additional, Randremanana, Rindra Vatosoa, additional, Mangahasimbola, Reziky, additional, Dziva Chikwari, Chido, additional, Kranzer, Katharina, additional, Mackworth-Young, Constance R S, additional, Bernays, Sarah, additional, Thomas, Nicola, additional, Anderson, David, additional, Tanko, Fatime Ramla, additional, Manhanzva, Monalisa, additional, Lurie, Micaela, additional, Khumalo, Fezile, additional, Sinanovic, Edina, additional, Honda, Ayako, additional, Pidwell, Tanya, additional, Francis, Suzanna C, additional, Masson, Lindi, additional, and Passmore, Jo-Anne, additional
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- 2024
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6. Inappropriate antibiotic prescribing and its determinants among outpatient children in 3 low- and middle-income countries: A multicentric community-based cohort study
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Ardillon, Antoine, Ramblière, Lison, Kermorvant-Duchemin, Elsa, Sok, Touch, Zo, Andrianirina Zafitsara, Diouf, Jean-Baptiste, Long, Pring, Lach, Siyin, Sarr, Fatoumata Diene, Borand, Laurence, Cheysson, Felix, Collard, Jean-Marc, Herindrainy, Perlinot, de Lauzanne, Agathe, Vray, Muriel, Delarocque-Astagneau, Elisabeth, Guillemot, Didier, and Huynh, Bich-Tram
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Drug resistance in microorganisms -- Prevention ,Prescription writing -- Methods ,Children -- Health aspects ,Ambulatory medical care -- Usage ,Antibiotics -- Dosage and administration ,Biological sciences - Abstract
Background Antibiotic resistance is a global public health issue, particularly in low- and middle-income countries (LMICs), where antibiotics required to treat resistant infections are not affordable. LMICs also bear a disproportionately high burden of bacterial diseases, particularly among children, and resistance jeopardizes progress made in these areas. Although outpatient antibiotic use is a major driver of antibiotic resistance, data on inappropriate antibiotic prescribing in LMICs are scarce at the community level, where the majority of prescribing occurs. Here, we aimed to characterize inappropriate antibiotic prescribing among young outpatient children and to identify its determinants in 3 LMICs. Methods and findings We used data from a prospective, community-based mother-and-child cohort (BIRDY, 2012 to 2018) conducted across urban and rural sites in Madagascar, Senegal, and Cambodia. Children were included at birth and followed-up for 3 to 24 months. Data from all outpatient consultations and antibiotics prescriptions were recorded. We defined inappropriate prescriptions as antibiotics prescribed for a health event determined not to require antibiotic therapy (antibiotic duration, dosage, and formulation were not considered). Antibiotic appropriateness was determined a posteriori using a classification algorithm developed according to international clinical guidelines. We used mixed logistic analyses to investigate risk factors for antibiotic prescription during consultations in which children were determined not to require antibiotics. Among the 2,719 children included in this analysis, there were 11,762 outpatient consultations over the follow-up period, of which 3,448 resulted in antibiotic prescription. Overall, 76.5% of consultations resulting in antibiotic prescription were determined not to require antibiotics, ranging from 71.5% in Madagascar to 83.3% in Cambodia. Among the 10,416 consultations (88.6%) determined not to require antibiotic therapy, 25.3% (n = 2,639) nonetheless resulted in antibiotic prescription. This proportion was much lower in Madagascar (15.6%) than in Cambodia (57.0%) or Senegal (57.2%) (p < 0.001). Among the consultations determined not to require antibiotics, in both Cambodia and Madagascar the diagnoses accounting for the greatest absolute share of inappropriate prescribing were rhinopharyngitis (59.0% of associated consultations in Cambodia, 7.9% in Madagascar) and gastroenteritis without evidence of blood in the stool (61.6% and 24.6%, respectively). In Senegal, uncomplicated bronchiolitis accounted for the greatest number of inappropriate prescriptions (84.4% of associated consultations). Across all inappropriate prescriptions, the most frequently prescribed antibiotic was amoxicillin in Cambodia and Madagascar (42.1% and 29.2%, respectively) and cefixime in Senegal (31.2%). Covariates associated with an increased risk of inappropriate prescription include patient age greater than 3 months (adjusted odds ratios (aOR) with 95% confidence interval (95% CI) ranged across countries from 1.91 [1.63, 2.25] to 5.25 [3.85, 7.15], p < 0.001) and living in rural as opposed to urban settings (aOR ranged across countries from 1.83 [1.57, 2.14] to 4.40 [2.34, 8.28], p < 0.001). Diagnosis with a higher severity score was also associated with an increased risk of inappropriate prescription (aOR = 2.00 [1.75, 2.30] for moderately severe, 3.10 [2.47, 3.91] for most severe, p < 0.001), as was consultation during the rainy season (aOR = 1.32 [1.19, 1.47], p < 0.001). The main limitation of our study is the lack of bacteriological documentation, which may have resulted in some diagnosis misclassification and possible overestimation of inappropriate antibiotic prescription. Conclusion In this study, we observed extensive inappropriate antibiotic prescribing among pediatric outpatients in Madagascar, Senegal, and Cambodia. Despite great intercountry heterogeneity in prescribing practices, we identified common risk factors for inappropriate prescription. This underscores the importance of implementing local programs to optimize antibiotic prescribing at the community level in LMICs., Author(s): Antoine Ardillon 1,2, Lison Ramblière 1,2, Elsa Kermorvant-Duchemin 3, Touch Sok 4, Andrianirina Zafitsara Zo 5, Jean-Baptiste Diouf 6, Pring Long 7, Siyin Lach 7, Fatoumata Diene Sarr 8, [...]
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- 2023
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7. Prevalence and Risk Factors for Colonisation During the First Three Months of Life with Three Critical Antibiotic-Resistant Pathogens In Low- and Middle-Income Countries: A Systematic Review, Meta-Analysis, and Meta-Regression Study
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Beaumont, Anne-Lise, primary, Breurec, Sébastien, additional, Kermorvant-Duchemin, Elsa, additional, and Huynh, Bich-Tram, additional
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- 2024
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8. A Novel Point-Of-Care Cytokine Biomarker Lateral Flow Test for the Screening for Sexually Transmitted Infections and Bacterial Vaginosis: Study Protocol of a Multi-Centre Multi-Disciplinary Prospective Clinical Study to Evaluate the Performance and Feasibility of the Genital Inflammation Test (GIFT)
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Ramboarina, Stéphanie, primary, Crucitti, Tania, additional, Gill, Katherine, additional, Bekker, Linda-Gail, additional, Harding-Esch, Emma M, additional, de Wijgert, Janneke HHM van, additional, Huynh, Bich-Tram, additional, Fortas, Camille, additional, Harimanana, Aina, additional, Gamana, Théodora Mayouya, additional, Randremanana, Rindra, additional, Mangahasimbola, Reziky, additional, Chikwari, Chido Dziva, additional, Kranzer, Katharina, additional, Mackworth-Young, Constance RS, additional, Bernays, Sarah, additional, Thomas, Nicola, additional, Anderson, David, additional, Tanko, Ramla Fatime, additional, Manhanzva, Monalisa, additional, Lurie, Micaela, additional, Khumalo, Fezile, additional, Sinanovic, Edina, additional, Honda, Ayako, additional, Pidwell, Tanya, additional, Francis, Suzanne, additional, Masson, Lindi, additional, and Passmore, Jo-Ann, additional
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- 2023
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9. PA-456 Field testing of a novel point-of-care Genital InFlammation Test (GIFT) in low- middle-income countries in Africa: aiming to prevent HIV infection and improve reproductive health
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Passmore, Jo-Ann, primary, Manhanzva, Monalisa, additional, Tanko, Ramla, additional, Pidwell, Tanya, additional, Khumalo, Fezile, additional, Lurie, Micaela, additional, Chikwari, Chido Dziva, additional, Mwaturura, Tinashe, additional, Kranzer, Katharina, additional, Mackworth-Young, Constance, additional, Kpokiri, Eneyi, additional, Bernays, Sarah, additional, Fortas, Camille, additional, Huynh, Bich-Tram, additional, Gill, Katherine, additional, Mahlangu, Karabo, additional, Randremanana, Rindra Vatosoa, additional, Gamana, Theodora Mayouya, additional, Harimanana, Aina, additional, Walt, Elise van der, additional, Sinanovic, Edina, additional, Honda, Ayako, additional, Marais, Saberi, additional, Wijgert, Janneke van de, additional, Bekker, Linda-Gail, additional, Anderson, David, additional, Harding-Esch, Emma, additional, Crucitti, Tania, additional, and Masson, Lindi, additional
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- 2023
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10. Bacterial vaginosis and other infections in pregnant women in Senegal
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Bonneton, Marion, Huynh, Bich-Tram, Seck, Abdoulaye, Bercion, Raymond, Sarr, Fatoumata Diene, Delarocque-Astagneau, Elisabeth, and Vray, Muriel
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- 2021
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11. Plugging the leaks: antibiotic resistance at human–animal interfaces in low‐resource settings
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Nadimpalli, Maya L, primary, Stegger, Marc, additional, Viau, Roberto, additional, Yith, Vuthy, additional, de Lauzanne, Agathe, additional, Sem, Nita, additional, Borand, Laurence, additional, Huynh, Bich‐tram, additional, Brisse, Sylvain, additional, Passet, Virginie, additional, Overballe‐Petersen, Søren, additional, Aziz, Maliha, additional, Gouali, Malika, additional, Jacobs, Jan, additional, Phe, Thong, additional, Hungate, Bruce A, additional, Leshyk, Victor O, additional, Pickering, Amy J, additional, Gravey, François, additional, Liu, Cindy M, additional, Johnson, Timothy J, additional, Le Hello, Simon, additional, and Price, Lance B, additional
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- 2023
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12. Combating Global Antibiotic Resistance : Emerging One Health Concerns in Lower- and Middle-Income Countries
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Bacterial Infections and antibiotic-Resistant Diseases among Young children in low-income countries (BIRDY) Study Group, Nadimpalli, Maya, Delarocque-Astagneau, Elisabeth, Love, David C., Price, Lance B., Huynh, Bich-Tram, Collard, Jean-Marc, Lay, Kruy Sun, Borand, Laurence, Ndir, Awa, Walsh, Timothy R., and Guillemot, Didier
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- 2018
13. Meat and Fish as Sources of Extended-Spectrum [beta]-Lactamase-Producing Escherichia coli, Cambodia
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Nadimpalli, Maya, Vuthy, Yith, de Lauzanne, Agathe, Fabre, Laetitia, Criscuolo, Alexis, Gouali, Malika, Huynh, Bich-Tram, Naas, Thierry, Phe, Thong, Borand, Laurence, Jacobs, Jan, Kerleguer, Alexandra, Piola, Patrice, Guillemot, Didier, Hello, Simon Le, and Delarocque-Astagneau, Elisabeth
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Lactams -- Contamination ,Beta lactamases ,Public health ,Escherichia coli ,Microbial drug resistance ,Backup software ,Aminoglycosides ,Hospital patients ,Resveratrol ,Cephalosporins ,Drug resistance ,Genocide ,Health - Abstract
In Europe, evidence for the spread of extended-spectrum [beta]-lactamase (ESBL)-producing Escherichia coli from animals to humans via food is unclear (1). Few studies have been conducted in low- and middle-income [...]
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- 2019
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14. Severe bacterial neonatal infections in Madagascar, Senegal, and Cambodia: A multicentric community-based cohort study
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Huynh, Bich-Tram, Kermorvant-Duchemin, Elsa, Chheang, Rattanak, Randrianirina, Frederique, Seck, Abdoulaye, Hariniaina Ratsima, Elisoa, Andrianirina, Zafitsara Zo, Diouf, Jean-Baptiste, Abdou, Armya Youssouf, Goyet, Sophie, Ngo, Véronique, Lach, Siyin, Pring, Long, Sok, Touch, Padget, Michael, Sarr, Fatoumata Diene, Borand, Laurence, Garin, Benoit, Collard, Jean-Marc, Herindrainy, Perlinot, de Lauzanne, Agathe, Vray, Muriel, Delarocque-Astagneau, Elisabeth, and Guillemot, Didier
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Infants -- Patient outcomes ,Infants (Newborn) -- Diseases ,Drug resistance in microorganisms -- Research ,Bacterial infections -- Statistics -- Risk factors -- Drug therapy ,Pediatric research ,Biological sciences - Abstract
Background Severe bacterial infections (SBIs) are a leading cause of neonatal deaths in low- and middle-income countries (LMICs). However, most data came from hospitals, which do not include neonates who did not seek care or were treated outside the hospital. Studies from the community are scarce, and few among those available were conducted with high-quality microbiological techniques. The burden of SBI at the community level is therefore largely unknown. We aimed here to describe the incidence, etiology, risk factors, and antibiotic resistance profiles of community-acquired neonatal SBI in 3 LMICs. Methods and findings The BIRDY study is a prospective multicentric community-based mother and child cohort study and was conducted in both urban and rural areas in Madagascar (2012 to 2018), Cambodia (2014 to 2018), and Senegal (2014 to 2018). All pregnant women within a geographically defined population were identified and enrolled. Their neonates were actively followed from birth to 28 days to document all episodes of SBI. A total of 3,858 pregnant women (2,273 (58.9%) in Madagascar, 814 (21.1%) in Cambodia, and 771 (20.0%) in Senegal) were enrolled in the study, and, of these, 31.2% were primigravidae. Women enrolled in the urban sites represented 39.6% (900/2,273), 45.5% (370/814), and 61.9% (477/771), and those enrolled in the rural sites represented 60.4% (1,373/2,273), 54.5% (444/814), and 38.1% (294/771) of the total in Madagascar, Cambodia, and Senegal, respectively. Among the 3,688 recruited newborns, 49.6% were male and 8.7% were low birth weight (LBW). The incidence of possible severe bacterial infection (pSBI; clinical diagnosis based on WHO guidelines of the Integrated Management of Childhood Illness) was 196.3 [95% confidence interval (CI) 176.5 to 218.2], 110.1 [88.3 to 137.3], and 78.3 [59.5 to 103] per 1,000 live births in Madagascar, Cambodia, and Senegal, respectively. The incidence of pSBI differed between urban and rural sites in all study countries. In Madagascar, we estimated an incidence of 161.0 pSBI per 1,000 live births [133.5 to 194] in the urban site and 219.0 [192.6 to 249.1] pSBI per 1,000 live births in the rural site (p = 0.008). In Cambodia, estimated incidences were 141.1 [105.4 to 189.0] and 85.3 [61.0 to 119.4] pSBI per 1,000 live births in urban and rural sites, respectively (p = 0.025), while in Senegal, we estimated 103.6 [76.0 to 141.2] pSBI and 41.5 [23.0 to 75.0] pSBI per 1,000 live births in urban and rural sites, respectively (p = 0.006). The incidences of culture-confirmed SBI were 15.2 [10.6 to 21.8], 6.5 [2.7 to 15.6], and 10.2 [4.8 to 21.3] per 1,000 live births in Madagascar, Cambodia, and Senegal, respectively, with no difference between urban and rural sites in each country. The great majority of early-onset infections occurred during the first 3 days of life (72.7%). The 3 main pathogens isolated were Klebsiella spp. (11/45, 24.4%), Escherichia coli (10/45, 22.2%), and Staphylococcus spp. (11/45, 24.4%). Among the 13 gram-positive isolates, 5 were resistant to gentamicin, and, among the 29 gram-negative isolates, 13 were resistant to gentamicin, with only 1 E. coli out of 10 sensitive to ampicillin. Almost one-third of the isolates were resistant to both first-line drugs recommended for the management of neonatal sepsis (ampicillin and gentamicin). Overall, 38 deaths occurred among neonates with SBI (possible and culture-confirmed SBI together). LBW and foul-smelling amniotic fluid at delivery were common risk factors for early pSBI in all 3 countries. A main limitation of the study was the lack of samples from a significant proportion of infants with pBSI including 35 neonatal deaths. Without these samples, bacterial infection and resistance profiles could not be confirmed. Conclusions In this study, we observed a high incidence of neonatal SBI, particularly in the first 3 days of life, in the community of 3 LMICs. The current treatment for the management of neonatal infection is hindered by antimicrobial resistance. Our findings suggest that microbiological diagnosis of SBI remains a challenge in these settings and support more research on causes of neonatal death and the implementation of early interventions (e.g., follow-up of at-risk newborns during the first days of life) to decrease the burden of neonatal SBI and associated mortality and help achieve Sustainable Development Goal 3., Author(s): Bich-Tram Huynh 1,2,*, Elsa Kermorvant-Duchemin 3, Rattanak Chheang 4, Frederique Randrianirina 5, Abdoulaye Seck 6, Elisoa Hariniaina Ratsima 5, Zafitsara Zo Andrianirina 7, Jean-Baptiste Diouf 8, Armya Youssouf Abdou [...]
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- 2021
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15. Fetal Growth Restriction Is Associated With Malaria in Pregnancy: A Prospective Longitudinal Study in Benin
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Briand, Valérie, Saal, Jessica, Ghafari, Caline, Huynh, Bich-Tram, Fievet, Nadine, Schmiegelow, Christentze, Massougbodji, Achille, Deloron, Philippe, Zeitlin, Jennifer, and Cot, Michel
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- 2016
16. Stillbirths and neonatal mortality in LMICs: A community-based mother-infant cohort study
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Rambliere, Lison, de Lauzanne, Agathe, Diouf, Jean-Baptiste, Zo, Andrianirina Zafitsara, Landau, Myriam, Herindrainy, Perlinot, Hivernaud, Delphine, Sarr, Fatoumata Diene, Sok, Touch, Vray, Muriel, Collard, Jean-Marc, Borand, Laurence, Delarocque-Astagneau, Elisabeth, Guillemot, Didier, Kermorvant-Duchemin, Elsa, Huynh, Bich-Tram, Study Group, Birdy, Epidémiologie et modélisation de la résistance aux antimicrobiens - Epidemiology and modelling of bacterial escape to antimicrobials (EMAE), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Centre Hospitalier Roi Baudouin Guédiawaye [Dakar, Senegal], Centre Hospitalier de Soavinandriana (CENHOSOA), Unité d’Épidémiologie et de Recherche clinique [Antananarivo, Madagascar], Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Cité (UPCité), Institut Pasteur de Dakar, Ministry of Health [Phnom Penh], Unité de Bactériologie Expérimentale [Antananarivo, Madagascar] (IPM), Johns Hopkins University School of Medicine [Baltimore], Hôpital Raymond Poincaré [Garches], and The present work was supported by internal resources from Paris-Sud University. The BIRDY 1 & 2 research projects were implemented with the financial support of the Monegasque Cooperation for development, the Total Foundation, and MSDAVENIR.
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[SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics - Abstract
International audience; BackgroundThe exact timing, causes, and circumstances of stillbirth and neonatal mortality in low- and middle-income countries (LMICs) remain poorly described, especially for antenatal stillbirths and deaths occurring at home. We aimed to provide reliable estimates of the incidence of stillbirth and neonatal death in three LMICs (Madagascar, Cambodia and Senegal) and to identify their main causes and associated risk factors.MethodsThis study is based on data from an international, multicentric, prospective, longitudinal, community-based mother-infant cohort. We included pregnant mothers and prospectively followed up their children in the community. Stillbirths and deaths were systematically reported; information across healthcare settings was collected and verbal autopsies were performed to document the circumstances and timing of death.ResultsAmong the 4436 pregnancies and 4334 live births, the peripartum period and the first day of life were the key periods of mortality. The estimated incidence of stillbirth was 11 per 1000 total births in Cambodia, 15 per 1000 in Madagascar, and 12 per 1000 in Senegal. We estimated neonatal mortality at 18 per 1000 live births in Cambodia, 24 per 1000 in Madagascar, and 23 per 1000 in Senegal. Based on ultrasound biometric data, 16.1% of infants in Madagascar were born prematurely, where 42% of deliveries and 33% of deaths occurred outside healthcare facilities. Risk factors associated with neonatal death were mainly related to delivery or to events that newborns faced during the first week of life.ConclusionsThese findings underscore the immediate need to improve care for and monitoring of children at birth and during early life to decrease infant mortality. Surveillance of stillbirth and neonatal mortality and their causes should be improved to mitigate this burden in LMICs.
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- 2023
17. Vaccination Coverage and Risk Factors Associated With Incomplete Vaccination Among Children in Cambodia, Madagascar, and Senegal
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Verrier, Florian, de Lauzanne, Agathe, Diouf, Jean-Baptiste Niokhhor, Zo, Andrianirina Zafitsara, Ramblière, Lison, Herindrainy, Perlinot, Sarr, Fatoumata Diene, Sok, Touch, Vray, Muriel, Collard, Jean-Marc, Borand, Laurence, Kermorvant-Duchemin, Elsa, Delarocque-Astagneau, Elisabeth, Guillemot, Didier, Huynh, Bich-Tram, Study Group, Bacterial Infections And Antibiotic-Resistant Diseases Among Young Children In Low-Income Countries, Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Epidémiologie et modélisation de la résistance aux antimicrobiens - Epidemiology and modelling of bacterial escape to antimicrobials (EMAE), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Centre Hospitalier Roi Baudouin Guédiawaye [Dakar, Senegal], Centre Hospitalier de Soavinandriana (CENHOSOA), Institut Pasteur de Madagascar, Institut Pasteur de Dakar, Ministry of Health [Phnom Penh], Johns Hopkins University School of Medicine [Baltimore], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Cité (UPCité), Hôpital Raymond Poincaré [Garches], Université Paris-Saclay, AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), This work was supported by the Department of International Cooperation of the Principality of Monaco, MSD Avenir, and Total Foundation., and BIRDY Study Group. Members from the Institut Pasteur in Madagascar include Aina Nirina Randriamamonjiarison, Tanjona Antsa Volahasina, Fanjalalaina Rasoanaivo, Feno Manitra Jacob Rakotoarimanana, Tanjona Bodonirina Raheliarivao, and Frédérique Randrianirina. Members from the Institut Pasteur in Cambodia include Thida Chon, Sophie Goyet, Alexandra Kerleguer, Véronique Ngo, Siyin Lach, Pring Long, and Arnaud Tarantola. Members from the Institut Pasteur in Senegal include Marguerite Diatta, Joseph Faye, and Abdoulaye Seck. Members from the Institut Pasteur in Paris include Michael Padget, Armiya Youssouf Abdou, and Benoit Garin.
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Infectious Diseases ,Oncology ,Madagascar ,risk factors ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,vaccine coverage ,Cambodia ,Senegal - Abstract
Background Vaccination reduces mortality from infectious disease, which is the leading cause of death in children under 5 and bears a particularly high burden in low- and middle-income countries. The Global Vaccine Action Plan (2011–2020) has set a target of 90% vaccine coverage for all vaccines included in national immunization programs by 2020. The objectives of this study were to estimate vaccine coverage among children in Madagascar, Cambodia, and Senegal and to identify the risk factors associated with incomplete vaccination. Methods Using data from a community-based prospective cohort that included all newborn of some areas from 2012 to 2018 in these 3 countries, vaccine coverage was estimated for BCG, hepatitis B, oral polio, pentavalent (targeting diphtheria, tetanus, pertussis, hepatitis B, and Haemophilus influenzae type b), and measles vaccines. Risk factor analysis was performed with logistic regression models to identify correlates of incomplete vaccination. Results A total of 3606 children were followed up, and vaccine coverage was below the 90% threshold for most vaccines in all countries. Coverage was higher for vaccines recommended at birth and at 6 weeks, while a decrease in coverage for subsequent doses was observed for vaccines requiring several doses (23–47 points). Low birth weight ( Conclusions Vaccine coverage for common childhood vaccines was lower than World Health Organization recommendations, and multidisciplinary approaches may help to improve vaccine coverage and timeliness.
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- 2023
18. Evaluating Antibacterial Effects of Bio-synthesized Silver-coated Silica Nanoparticles on Staphylococcus aureusand Acinetobacter baumannii
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Nguyen, Thuy-Trinh Thi, Dang, Quoc-Dat, Huynh, Bich-Tram Thi, Le, Khanh-Vi Thi, Nguyen, Tan Tai, and Tran-Van, Hieu
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Healthcare-associated infections (HAIs) cause economic and social burden by affecting millions of patients annually, especially in low-income countries. The treatment of HAIs with antibiotics can lead to their increased resistance and subsequently increased mortality. As an alternative solution, nanomaterials combined with inorganic metals (silver ions and silver-based compounds) have been proposed. In this study, after extraction of sodium silicate from rice husk ash, silver-coated silica nanoparticles (SiO2@Ag-NPs) were synthesized by sol-gel method and evaluated the antibacterial activity against hospital-acquired infections. SiO2@Ag-NPs were analyzed by transmission electron microscopy (TEM). The antibacterial activity of SiO2@Ag-NPs against two clinical isolated bacterial strains, including methicillin-resistant Staphylococcus aureusand Acinetobacter baumannii, was assessed using minimum inhibitory concentrations (MICs) and using scanning electron microscopy (SEM). TEM images showed that SiO2nanoparticles had a uniform shape with a size of around 50 nm SiO2@Ag-NPs’ surface area and homogeneous pores are 78 m2/g and 2.71 nm, respectively, with purity over 98%. Silver nanoparticles attached to SiO2surface have uniform shapes with size smaller than 5 nm. The MIC results were determined from 25 to 400 ppm depending on the concentration and bacterial strain tested. SEM images showed that SiO2@Ag-NPs inhibited established biofilms in both bacterial strains, discrete bacterial cells, and fewer clusters. There was no or little extracellular matrix observed, the cells were distorted, shrank, ruptured, and appeared to have indentation or perforation in the cell wall. The results showed that SiO2@Ag-NPs were an attractive nanomaterial for commercial applications and in treating diseases associated with microbial infections.
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- 2024
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19. Hospital contact patterns and vulnerability to SARS-CoV-2 outbreaks
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Shirreff, George, primary, Huynh, Bich-Tram, additional, Duval, Audrey, additional, Pereira, Lara Cristina, additional, Annane, Djillali, additional, Dinh, Aurélien, additional, Lambotte, Olivier, additional, Bulifon, Sophie, additional, Guichardon, Magali, additional, Beaune, Sebastien, additional, Toubiana, Julie, additional, Kermovant-Duchemin, Elsa, additional, Chéron, Gerard, additional, Cordel, Hugues, additional, Argaud, Laurent, additional, Douplat, Marion, additional, Abraham, Paul, additional, Tazarourte, Karim, additional, Martin-Gaujard, Géraldine, additional, Vanhems, Philippe, additional, Hilliquin, Delphine, additional, Nguyen, Duc, additional, Chelius, Guillaume, additional, Fraboulet, Antoine, additional, Temime, Laura, additional, Opatowski, Lulla, additional, and Guillemot, Didier, additional
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- 2022
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20. Neonatal acquisition of extended-spectrum beta-lactamase-producing Enterobacteriaceae in the community of a low-income country (NeoLIC): protocol for a household cohort study in Moramanga, Madagascar
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Harimanana, Aina, primary, Rakotondrasoa, Andriniaina, additional, Rivoarilala, Lalainasoa Odile, additional, Criscuolo, Alexis, additional, Opatowski, Lulla, additional, Rakotomanana, Elliot Fara Nandrasana, additional, Herindrainy, Perlinot, additional, Collard, Jean-Marc, additional, Crucitti, Tania, additional, and Huynh, Bich-Tram, additional
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- 2022
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21. A community survey of antibiotic consumption among children in Madagascar and Senegal: the importance of healthcare access and care quality
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Padget, Michael, Tamarelle, Jeanne, Herindrainy, Perlinot, Ndir, Awa, Diene Sarr, Fatoumata, Richard, Vincent, Piola, Patrice, Guillemot, Didier, Delarocque-Astagneau, Elisabeth, Seguy, Maud, Cherblanc, Fanny, Watier, Laurence, Nadimpalli, Maya, Le Fouler, Lenaig, Garin, Benoit, Huynh, Bich-Tram, Collard, Jean-Marc, Raheliarivao, Bodonirina Tanjona, Rakotoarimanana, Feno Manitra Jacob, Randrianirina, Frédérique, Vray, Muriel, Seck, Abdoulaye, Bercion, Raymond, Sow, Amy Gassama, Diouf, Jean Baptiste, Dieye, Pape Samba, Sy, Balla, Ndao, Bouya, Borand, Laurence, Chon, Thida, de Lauzanne, Agathe, Goyet, Sophie, Kerleguer, Alexandra, Lach, Siyin, Ngo, Veronique, Tarantola, Arnaud, Touch, Sok, and Kermorvant-Duchemin, Elsa
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- 2017
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22. Excess risk of subsequent infection in hospitalized children from a community cohort study in Cambodia and Madagascar
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Rambliere, Lison, primary, Kermorvant-Duchemin, Elsa, additional, de Lauzanne, Agathe, additional, Collard, Jean-Marc, additional, Herindrainy, Perlinot, additional, Vray, Muriel, additional, Garin, Benoit, additional, Zo, Andrianirina Zafitsara, additional, Rasoanaivo, Fanjalalaina, additional, Rakotoarimanana Feno Manitra, Jacob, additional, Raheliarivao, Tanjona Bodonirina, additional, Diouf, Jean-Baptiste Niokhhor, additional, Ngo, Véronique, additional, Lach, Siyin, additional, Long, Pring, additional, Borand, Laurence, additional, Sok, Touch, additional, Abdou, Armiya Youssouf, additional, Padget, Michael, additional, Madec, Yoann, additional, Guillemot, Didier, additional, Delarocque-Astagneau, Elisabeth, additional, and Huynh, Bich-Tram, additional
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- 2022
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23. Lockdown impact on age-specific contact patterns and behaviours, France, April 2020
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Bosetti, Paolo, primary, Huynh, Bich-Tram, additional, Abdou, Armiya Youssouf, additional, Sanchez, Marie, additional, Eisenhauer, Catherine, additional, Courtejoie, Noémie, additional, Accardo, Jérôme, additional, Salje, Henrik, additional, Guillemot, Didier, additional, Moslonka-Lefebvre, Mathieu, additional, Boëlle, Pierre-Yves, additional, Béraud, Guillaume, additional, Cauchemez, Simon, additional, and Opatowski, Lulla, additional
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- 2021
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24. Prevalence and Factors Associated with Maternal Group B Streptococcus Colonization in Madagascar and Senegal
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Jung, Yu-Jin, primary, Huynh, Bich-Tram, additional, Seck, Abdoulaye, additional, Bercion, Raymond, additional, Sarr, Fatoumata Diene, additional, Herindrainy, Perlinot, additional, Diouf, Jean-Baptiste, additional, Andrianirina, Zafitsara Zo, additional, Firon, Arnaud, additional, Trieu-Cuot, Patrick, additional, Goyet, Sophie, additional, Collard, Jean-Marc, additional, Delarocque-Astagneau, Elisabeth, additional, Guillemot, Didier, additional, Vray, Muriel, additional, and _, _, additional
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- 2021
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25. What is the impact of mass and systematic antibiotic administration on antibiotic resistance in low- and middle-income countries? A systematic review
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Rambliere, Lison, Guillemot, Didier, Delarocque-Astagneau, Elisabeth, Huynh, Bich-Tram, Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Epidémiologie et modélisation de la résistance aux antimicrobiens - Epidemiology and modelling of bacterial escape to antimicrobials (EMAE), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), AP-HP. Université Paris Saclay, The work was supported directly by internal resources from University Paris-Sud., We thank the scientific information resources center (CERIS) of the Pasteur Institut for assisting in the search strategy and David RM Smith for his critical review of the article and proofreading in English., Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Bich-Tram, Huynh
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[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,Antibiotic usage ,Public health ,mass drug administration ,systematic drug administration ,[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie ,Antibiotic resistance ,[SDV.EE.SANT] Life Sciences [q-bio]/Ecology, environment/Health ,global health ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,low- and middle-income countries ,prophylaxis - Abstract
International audience; Antibiotic consumption is a key driver of antibiotic resistance (AR), particularly in low- and middle-income countries, where risk factors for AR emergence and spread are rife. However, the potential contribution of mass and systematic antibiotic administration (MDA/SDA) to AR spread is unknown. We conducted a systematic review to provide an overview of MDA/SDA in low- and middle-income countries, including indications, antibiotics used and, if investigated, levels of AR over time. This systematic review is reported in accordance with the PRISMA statement. Of 2438 identified articles, 63 were reviewed: indications for MDA/SDA were various, and targeted populations were particularly vulnerable, including pregnant women, children, HIV-infected populations and communities in outbreak settings. Available data suggest MDA/SDA may lead to significant AR increase, especially after azithromycin administration. However, only 40% of studies evaluated AR. Integrative approaches that evaluate AR in addition to clinical outcomes are needed to understand consequences of MDA/SDA implementation, combined with standardized AR surveillance for timely detection of antibiotic resistance emergence.
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- 2021
26. Erratum to “Impact of mass and systematic antibiotic administration on antibiotic resistance in low- and middle-income countries. A systematic review” [International Journal of Antimicrobial Agents (2021) Volume 58, Issue 1/106364]
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Ramblière, Lison, primary, Guillemot, Didier, additional, Delarocque-Astagneau, Elisabeth, additional, and Huynh, Bich-Tram, additional
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- 2021
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27. Impact of mass and systematic antibiotic administration on antibiotic resistance in low- and middle-income countries. A systematic review
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Ramblière, Lison, primary, Guillemot, Didier, additional, Delarocque-Astagneau, Elisabeth, additional, and Huynh, Bich-Tram, additional
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- 2021
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28. Leakiness at the human-animal interface in Southeast Asia and implications for the spread of antibiotic resistance
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Nadimpalli, Maya L., primary, Stegger, Marc, additional, Viau, Roberto, additional, Yith, Vuthy, additional, de Lauzanne, Agathe, additional, Sem, Nita, additional, Borand, Laurence, additional, Huynh, Bich-tram, additional, Brisse, Sylvain, additional, Passet, Virginie, additional, Overballe-Petersen, Søren, additional, Aziz, Maliha, additional, Gouali, Malika, additional, Jacobs, Jan, additional, Phe, Thong, additional, Hungate, Bruce A., additional, Leshyk, Victor O., additional, Pickering, Amy J., additional, Gravey, François, additional, Liu, Cindy M., additional, Johnson, Timothy J., additional, Hello, Simon Le, additional, and Price, Lance B., additional
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- 2021
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29. How best to use limited tests? Improving COVID-19 surveillance in long-term care
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Smith, David RM, Duval, Audrey, Pouwels, Koen B, Guillemot, Didier, Fernandes, Jerome, Huynh, Bich-Tram, Temime, Laura, and Opatowski, Lulla
- Abstract
Background: Long-term care facilities (LTCFs) are particularly vulnerable to nosocomial outbreaks of coronavirus disease 2019 (COVID-19), with high rates of transmission and mortality. Timely epidemiological surveillance is essential to detect and respond to outbreaks, but testing resources are highly limited in the current pandemic context. Methods: We used an individual-based transmission model to simulate COVID-19 spread along inter-individual contact networks in the LTCF setting. A range of surveillance strategies were evaluated for their ability to detect simulated outbreaks, assuming limited availability of standard RT-PCR tests. Various epidemiological scenarios were considered, including COVID-19 importation from patient transfers or staff members infected in the community. Findings: We estimated a median delay of 7 (95% uncertainty interval: 2-15) days from importation of an asymptomatic COVID-19-infected patient to first presentation of COVID-19 symptoms among any patients or staff, at which point an additional 7 (0-25) individuals were infected but did not (yet) show symptoms. Across a range of scenarios, the reference surveillance strategy (testing individuals with COVID-like symptoms with signs of severity) took a median 11-21 days to detect an outbreak. Group testing (pooling specimens from multiple individuals for a single RT-PCR test) patients and staff with any COVID-like symptoms was both the most timely and efficient strategy, detecting outbreaks up to twice as quickly as the reference, and more quickly than other considered strategies while using fewer tests. Maximizing use of available tests via testing cascades was more effective than group testing only when substantial testing resources were available (on the order of 1 test/20 beds/day). Including not merely those with symptoms but also newly admitted patients in group tests and testing cascades reduced delays in outbreak detection for LTCFs actively admitting patients potentially already infected with COVID-19. Interpretation: Improving COVID-19 surveillance can alert healthcare institutions to emerging outbreaks before they escalate, informing a need for urgent public health intervention in settings with ongoing nosocomial transmission.
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- 2020
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30. Bacterial Vaginosis and Risk Factors in Pregnant Women in Senegal
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Bonneton, Marion, primary, Huynh, Bich-Tram, additional, Seck, Abdoulaye, additional, Bercion, Raymond, additional, Sarr, Fatoumata Diene, additional, Delarocque-Astagneau, Elisabeth, additional, and Vray, Muriel, additional
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- 2021
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31. Excess Risk of Subsequent Infection in Hospitalized Children: Evidence from a Community Cohort Study in Cambodia and Madagascar
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Rambliere, Lison, primary, Kermorvant-Duchemin, Elsa, additional, De Lauzanne, Agathe, additional, Collard, Jean-Marc, additional, Herindrainy, Perlinot, additional, Vray, Muriel, additional, Garin, Benoit, additional, Zo, Andrianirina Zafitsara, additional, Rasoanaivo, Fanjalalaina, additional, Rakotoarimanana, Feno Manitra Jacob, additional, Raheliarivao, Tanjona Bodonirina, additional, Diouf, Jean Baptiste Niokhhor, additional, Ngo, Véronique, additional, Lach, Siyin, additional, Long, Pring, additional, Borand, Laurence, additional, Sok, Touch, additional, Abdou, Armiya Youssouf, additional, Padget, Michael, additional, Madec, Yoann, additional, Guillemot, Didier, additional, Delarocque-Astagneau, Elisabeth, additional, and Huynh, Bich-Tram, additional
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- 2021
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32. Lockdown impact on age-specific contact patterns and behaviours in France
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Bosetti, Paolo, primary, Huynh, Bich-Tram, additional, Abdou, Armiya Youssouf, additional, Sanchez, Marie, additional, Eisenhauer, Catherine, additional, Courtejoie, Noémie, additional, Accardo, Jérôme, additional, Salje, Henrik, additional, Guillemot, Didier, additional, Moslonka-Lefebvre, Mathieu, additional, Boëlle, Pierre-Yves, additional, Béraud, Guillaume, additional, Cauchemez, Simon, additional, and Opatowski, Lulla, additional
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- 2020
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33. Klebsiella pneumoniae carriage in low-income countries: antimicrobial resistance, genomic diversity and risk factors
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Huynh, Bich-Tram, primary, Passet, Virginie, additional, Rakotondrasoa, Andriniaina, additional, Diallo, Thierno, additional, Kerleguer, Alexandra, additional, Hennart, Melanie, additional, Lauzanne, Agathe De, additional, Herindrainy, Perlinot, additional, Seck, Abdoulaye, additional, Bercion, Raymond, additional, Borand, Laurence, additional, Pardos de la Gandara, Maria, additional, Delarocque-Astagneau, Elisabeth, additional, Guillemot, Didier, additional, Vray, Muriel, additional, Garin, Benoit, additional, Collard, Jean-Marc, additional, Rodrigues, Carla, additional, and Brisse, Sylvain, additional
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- 2020
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34. Optimizing COVID-19 surveillance in long-term care facilities: a modelling study
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Smith, David RM, primary, Duval, Audrey, additional, Pouwels, Koen B, additional, Guillemot, Didier, additional, Fernandes, Jérôme, additional, Huynh, Bich-Tram, additional, Temime, Laura, additional, and Opatowski, Lulla, additional
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- 2020
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35. Characterization of Klebsiella pneumoniae isolates from a mother–child cohort in Madagascar
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Rakotondrasoa, Andriniaina, primary, Passet, Virginie, primary, Herindrainy, Perlinot, primary, Garin, Benoit, primary, Kermorvant-Duchemin, Elsa, primary, Delarocque-Astagneau, Elisabeth, primary, Guillemot, Didier, primary, Huynh, Bich-Tram, primary, Brisse, Sylvain, primary, and Collard, Jean-Marc, primary
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- 2020
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36. P468: Sexually Transmitted Infections and Bacterial Vaginosis among Women of Child-Bearing Age in Antananarivo, Madagascar: Prevalence and Risk Factors.
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Fortas, Camille, Crucitti, Tania, Harimanana, Aina, Huynh, Bich-Tram, Rafetrarivony, Lala, Randremanana, Rindra Vatosoa, and Rasoanandrianina, Solange Bienvenue
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- 2024
37. Malaria and gravidity interact to modify maternal haemoglobin concentrations during pregnancy
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Ouédraogo Smaïla, Bodeau-Livinec Florence, Briand Valérie, Huynh Bich-Tram, Koura Ghislain K, Accrombessi Manfred MK, Fievet Nadine, Massougbodji Achille, Deloron Philippe, and Cot Michel
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Anaemia ,Gravidity ,Malaria ,Iron deficiency ,Prevention ,Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Primigravidity is one of the main risk factors for both malaria and anaemia. Since the implementation of intermittent preventive treatment (IPTp) in sub-Saharan Africa, the relationship between anaemia and gravidity and its evolution during pregnancy has been little explored. This study aimed to evaluate the impact of gravidity on the variation of haemoglobin during pregnancy according to the timing of gestation. Methods Data from three studies carried out in nearby areas in south Benin (Ouidah, Comé, Allada) between 2005 and 2012 were analysed. At inclusion (first antenatal visit, ANV1) women’s age, area of residence, schooling, gravidity, gestational age, weight and height were recorded. Thick blood smears were performed on ANV1, second visit (ANV2) and at delivery. In Allada, women’s serum ferritin and CRP concentrations were also assessed. The impact of gravidity on maternal haemoglobin (Hb) was analysed using a logistic or linear regression depending on the outcome. The statistical significance was set to P < 0.05. Results In total, data from 3,591 pregnant women were analysed. Both univariate and multivariate analyses showed a constant association between Hb concentrations and gravidity in the three periods of Hb assessment (ANV1, ANV2 and delivery). Mean Hb concentration was significantly lower in primigravidae than in multigravidae at ANV1 (mean difference = -2.4 g/L, CI 95%: [-3.4, -1.4], P < 0.001). Afterwards, there was a significant increase in primigravidae only, with a tendency to reversal between primigravidae and multigravidae, which was confirmed at delivery (mean difference = 2.8 g/L, CI 95%: [1.3, 4.2], P < 0.001). The prevalence of malaria infection was halved between ANV1 and delivery in primigravidae while it decreased by only 38% among multigravidae, who were less prone to malaria infection (prevalence at ANV1, 20% and 10% respectively). Iron deficiency was more common in multigravidae, and it decreased slightly in this group between ANV1 and delivery. Conclusion In a context of IPTp, Hb levels improved progressively throughout pregnancy in primigravidae, likely as a result of reduction in malaria infection. In multigravidae, the improvement was less perceptible and anaemia was mainly due to iron deficiency.
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- 2012
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38. Inference of significant microbial interactions from longitudinal metagenomics sequencing data
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Gao, Xuefeng, Huynh, Bich-Tram, Guillemot, Didier, Glaser, Philippe, and Opatowski, Lulla
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Clostridia ,Genetics ,education.field_of_study ,Bacteroidia ,Microbial population biology ,biology ,Metagenomics ,Population ,Gammaproteobacteria ,Microbiome ,Gut flora ,biology.organism_classification ,education - Abstract
Data of next-generation sequencing (NGS) and their analysis have been facilitating advances in our understanding of microbial ecosystems such as human gut microbiota. However, inference of microbial interactions occurring within an ecosystem is still a challenge mainly due to metagenomics sequencing (e.g., 16S rDNA sequences) providing relative abundance of microbes instead of absolute cell count. In order to describe the population dynamics in microbial communities and estimate the involved microbial interactions, we introduce a procedure by integrating generalized Lotka-Volterra equations, forward stepwise regression and bootstrap aggregation. First, we successfully identify experimentally confirmed microbial interactions with relative abundance data of a cheese microbial community. Then, we apply the procedure to time-series of 16S rDNA sequences of gut microbiomes of children who were progressing to Type 1 diabetes (T1D progressors), and compare their gut microbial interactions to a healthy control group. Our results suggest that the number of inferred microbial interactions increased over time during the first three years of life. More microbial interactions are found in the gut flora of healthy children than the T1D progressors. The inhibitory effects from Actinobacteria and Bacilli to Bacteroidia, from Bacteroidia to Clostridia, and the benifit effect from Clostridia to Bacteroidia are shared between healthy children and T1D progressors. An inhibition of Clostridia by Gammaproteobacteria is found in healthy children that maintains through their first three years of life. This suppression appears in T1D progressors during the first year of life, which transforms to a commensalism relationship at the age of three years old. Gammaproteobacteria is found exerting an inhibition on Bacteroidia in the T1D progressors, which is not identified in the healthy controls.
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- 2018
39. Combating Global Antibiotic Resistance: Emerging One Health Concerns in Lower- and Middle-Income Countries
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Nadimpalli, Maya, Delarocque-Astagneau, Elisabeth, Love, David C, Price, Lance B, Huynh, Bich-Tram, Collard, Jean-Marc, Lay, Kruy Sun, Borand, Laurence, Ndir, Awa, Walsh, Timothy R, Guillemot, Didier, De Lauzanne, Agathe, Kerleguer, Alexandra, Tarantola, Arnaud, Piola, Patrice, Chon, Thida, Lach, Siyin, Ngo, Veronique, Touch, Sok, Andrianirina, Zo Zafitsara, Vray, Muriel, Richard, Vincent, Seck, Abdoulaye, Bercion, Raymond, Sow, Amy Gassama, Diouf, Jean Baptiste, Dieye, Pape Samba, Sy, Balla, Ndao, Bouya, Seguy, Maud, Watier, Laurence, Abdou, Armiya Youssouf, Biostatistique, Biomathématique, Pharmacoépidémiologie et Maladies Infectieuses (B2PHI), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Johns Hopkins Bloomberg School of Public Health [Baltimore], Johns Hopkins University (JHU), Pharmacoepidemiologie et évaluation de l'impact des produits de santé sur les populations, Université Bordeaux Segalen - Bordeaux 2-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Bactériologie Expérimentale [Antananarivo, Madagascar] (IPM), Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Unité d'Épidémiologie et de Santé Publique [Phnom Penh], Institut Pasteur de Dakar, Université du Québec à Montréal = University of Québec in Montréal (UQAM), Unité d'Epidémiologie [Antananarivo, Madagascar] (IPM), Communicable Disease Department [Phnom Penh] (CDC MOH), Ministry of Health [Mozambique], Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Institut Pasteur de Bangui, IDEFI-N-Reflexpro, Fonctionnement, évolution et mécanismes régulateurs des écosystèmes forestiers (ECOTROP), Centre National de la Recherche Scientifique (CNRS)-Muséum national d'Histoire naturelle (MNHN), Bacterial Infections and antibiotic-Resistant Diseases among Young children in low-income countries (BIRDY) Study Group., Tarantola, Arnaud, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), and Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,Microbiology (medical) ,Environmental pollution ,Drug resistance ,03 medical and health sciences ,0302 clinical medicine ,Antibiotic resistance ,Drug Misuse ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Development economics ,Medicine ,Humans ,030212 general & internal medicine ,One Health ,Developing Countries ,Poverty ,2. Zero hunger ,business.industry ,Middle income countries ,Drug Resistance, Microbial ,Antibiotic misuse ,Food safety ,3. Good health ,Anti-Bacterial Agents ,030104 developmental biology ,Infectious Diseases ,13. Climate action ,[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Income ,business ,Environmental Pollution - Abstract
International audience; Antibiotic misuse in lower- and middle-income countries (LMICs) contributes to the development of antibiotic resistance that can disseminate globally. Strategies specific to LMICs that seek to reduce antibiotic misuse by humans, but simultaneously improve antibiotic access, have been proposed. However, most approaches to date have not considered the growing impact of animal and environmental reservoirs of antibiotic resistance, which threaten to exacerbate the antibiotic resistance crisis in LMICs. In particular, current strategies do not prioritize the impacts of increased antibiotic use for terrestrial food-animal and aquaculture production, inadequate food safety, and widespread environmental pollution. Here, we propose new approaches that address emerging, One Health challenges.
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- 2018
40. Clonal transmission and new mechanism of resistance to trimethoprim-sulfamethoxazole in Stenotrophomonas maltophilia strains isolated in a neonatology unit at Antananarivo, Madagascar, deciphered by whole genome sequence analysis
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Noah Rabenandrasana, Mamitina Alain, primary, Andrianoelina, Volasoa, additional, Bonneault, Melanie, additional, Herindrainy, Perlinot, additional, Garin, Benoit, additional, Breurec, Sebastien, additional, Delarocque-Astagneau, Elisabeth, additional, Andrianirina, Zafitsara Zo, additional, Enouf, Vincent, additional, Huynh, Bich-Tram, additional, Opatowski, Lulla, additional, and Collard, Jean-Marc, additional
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- 2019
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41. Transmission Routes of Extended-Spectrum Beta-Lactamase–Producing Enterobacteriaceae in a Neonatology Ward in Madagascar
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Bonneault, Mélanie, primary, Andrianoelina, Volasoa Herilalaina, additional, Herindrainy, Perlinot, additional, Rabenandrasana, Mamitina Alain Noah, additional, Garin, Benoit, additional, Breurec, Sebastien, additional, Delarocque-Astagneau, Elisabeth, additional, Guillemot, Didier, additional, Andrianirina, Zafitsara Zo, additional, Collard, Jean-Marc, additional, Huynh, Bich-Tram, additional, and Opatowski, Lulla, additional
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- 2019
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42. Malaria associated symptoms in pregnant women followed-up in Benin
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Massougbodji Achille, Mévo Blaise, Borgella Sophie, Gbaguidi Gildas, Fievet Nadine, Huynh Bich-Tram, Deloron Philippe, and Cot Michel
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background It is generally agreed that in high transmission areas, pregnant women have acquired a partial immunity to malaria and when infected they present few or no symptoms. However, longitudinal cohort studies investigating the clinical presentation of malaria infection in pregnant women in stable endemic areas are lacking, and the few studies exploring this issue are unconclusive. Methods A prospective cohort of women followed monthly during pregnancy was conducted in three rural dispensaries in Benin from August 2008 to September 2010. The presence of symptoms suggestive of malaria infection in 982 women during antenatal visits (ANV), unscheduled visits and delivery were analysed. A multivariate logistic regression was used to determine the association between symptoms and a positive thick blood smear (TBS). Results During routine ANVs, headache was the only symptom associated with a higher risk of positive TBS (aOR = 1.9; p < 0.001). On the occasion of unscheduled visits, fever (aOR = 5.2; p < 0.001), headache (aOR = 2.1; p = 0.004) and shivering (aOR = 3.1; p < 0.001) were significantly associated with a malaria infection and almost 90% of infected women presented at least one of these symptoms. Two thirds of symptomatic malaria infections during unscheduled visits occurred in late pregnancy and long after the last intermittent preventive treatment dose (IPTp). Conclusion The majority of pregnant women were symptomless during routine visits when infected with malaria in an endemic stable area. The only suggestive sign of malaria (fever) was associated with malaria only on the occasion of unscheduled visits. The prevention of malaria in pregnancy could be improved by reassessing the design of IPTp, i.e. by determining an optimal number of doses and time of administration of anti-malarial drugs.
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- 2011
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43. Optimizing COVID-19 surveillance in long-term care facilities: a modelling study.
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Smith, David R. M., Duval, Audrey, Pouwels, Koen B., Guillemot, Didier, Fernandes, Jérôme, Huynh, Bich-Tram, Temime, Laura, Opatowski, Lulla, and AP-HP/Universities/Inserm COVID-19 research collaboration
- Subjects
LONG-term care facilities ,REVERSE transcriptase polymerase chain reaction ,COVID-19 - Abstract
Background: Long-term care facilities (LTCFs) are vulnerable to outbreaks of coronavirus disease 2019 (COVID-19). Timely epidemiological surveillance is essential for outbreak response, but is complicated by a high proportion of silent (non-symptomatic) infections and limited testing resources.Methods: We used a stochastic, individual-based model to simulate transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) along detailed inter-individual contact networks describing patient-staff interactions in a real LTCF setting. We simulated distribution of nasopharyngeal swabs and reverse transcriptase polymerase chain reaction (RT-PCR) tests using clinical and demographic indications and evaluated the efficacy and resource-efficiency of a range of surveillance strategies, including group testing (sample pooling) and testing cascades, which couple (i) testing for multiple indications (symptoms, admission) with (ii) random daily testing.Results: In the baseline scenario, randomly introducing a silent SARS-CoV-2 infection into a 170-bed LTCF led to large outbreaks, with a cumulative 86 (95% uncertainty interval 6-224) infections after 3 weeks of unmitigated transmission. Efficacy of symptom-based screening was limited by lags to symptom onset and silent asymptomatic and pre-symptomatic transmission. Across scenarios, testing upon admission detected just 34-66% of patients infected upon LTCF entry, and also missed potential introductions from staff. Random daily testing was more effective when targeting patients than staff, but was overall an inefficient use of limited resources. At high testing capacity (> 10 tests/100 beds/day), cascades were most effective, with a 19-36% probability of detecting outbreaks prior to any nosocomial transmission, and 26-46% prior to first onset of COVID-19 symptoms. Conversely, at low capacity (< 2 tests/100 beds/day), group testing strategies detected outbreaks earliest. Pooling randomly selected patients in a daily group test was most likely to detect outbreaks prior to first symptom onset (16-27%), while pooling patients and staff expressing any COVID-like symptoms was the most efficient means to improve surveillance given resource limitations, compared to the reference requiring only 6-9 additional tests and 11-28 additional swabs to detect outbreaks 1-6 days earlier, prior to an additional 11-22 infections.Conclusions: COVID-19 surveillance is challenged by delayed or absent clinical symptoms and imperfect diagnostic sensitivity of standard RT-PCR tests. In our analysis, group testing was the most effective and efficient COVID-19 surveillance strategy for resource-limited LTCFs. Testing cascades were even more effective given ample testing resources. Increasing testing capacity and updating surveillance protocols accordingly could facilitate earlier detection of emerging outbreaks, informing a need for urgent intervention in settings with ongoing nosocomial transmission. [ABSTRACT FROM AUTHOR]- Published
- 2020
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44. Inference of Significant Microbial Interactions From Longitudinal Metagenomics Data
- Author
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Gao, Xuefeng, primary, Huynh, Bich-Tram, additional, Guillemot, Didier, additional, Glaser, Philippe, additional, and Opatowski, Lulla, additional
- Published
- 2018
- Full Text
- View/download PDF
45. Inference of significant microbial interactions from longitudinal metagenomics sequencing data
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Gao, Xuefeng, primary, Huynh, Bich-Tram, additional, Guillemot, Didier, additional, Glaser, Philippe, additional, and Opatowski, Lulla, additional
- Published
- 2018
- Full Text
- View/download PDF
46. Bacterial Infections in Neonates, Madagascar, 2012–2014
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Huynh, Bich-Tram, primary, Kermorvant-Duchemin, Elsa, additional, Herindrainy, Perlinot, additional, Padget, Michael, additional, Rakotoarimanana, Feno Manitra Jacob, additional, Feno, Herisoa, additional, Hariniaina-Ratsima, Elisoa, additional, Raheliarivao, Tanjona, additional, Ndir, Awa, additional, Goyet, Sophie, additional, Piola, Patrice, additional, Randrianirina, Frederique, additional, Garin, Benoit, additional, Collard, Jean-Marc, additional, Guillemot, Didier, additional, and Delarocque-Astagneau, Elisabeth, additional
- Published
- 2018
- Full Text
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47. Extended Spectrum β-Lactamase-Producing Enterobacteriaceae among Meat, Fish, and Healthy Mothers in Phnom Penh, Cambodia
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Nadimpalli, Maya, primary, Lay, Kruy S., additional, Vuthy, Yith, additional, Gouali, Malika, additional, de Lauzanne, Agathe, additional, Borand, Laurence, additional, Hello, Simon Le, additional, Fabre, Laétitia, additional, Huynh, Bich-tram, additional, and Delarocque-Astagneau, Elisabeth, additional
- Published
- 2018
- Full Text
- View/download PDF
48. P357: Proportion of Asymptomatic Sexually Transmitted Infections in Women in Low- and Middle-Income Countries: A Systematic Review and Meta-Analysis.
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Fortas, Camille, Crucitti, Tania, Huynh, Bich-Tram, and Randremanana, Rindra Vatosoa
- Published
- 2024
49. Klebsiella pneumoniaecarriage in low-income countries: antimicrobial resistance, genomic diversity and risk factors
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Huynh, Bich-Tram, Passet, Virginie, Rakotondrasoa, Andriniaina, Diallo, Thierno, Kerleguer, Alexandra, Hennart, Melanie, Lauzanne, Agathe De, Herindrainy, Perlinot, Seck, Abdoulaye, Bercion, Raymond, Borand, Laurence, Pardos de la Gandara, Maria, Delarocque-Astagneau, Elisabeth, Guillemot, Didier, Vray, Muriel, Garin, Benoit, Collard, Jean-Marc, Rodrigues, Carla, and Brisse, Sylvain
- Abstract
ABSTRACTBackgroundKlebsiella pneumoniae(hereafter, Kp) is a major public health threat responsible for high levels of multidrug resistant (MDR) human infections. Besides, Kp also causes severe infections in the community, especially in Asia and Africa. Although most Kp infections are caused by endogenous intestinal carriage, little is known about the prevalence and microbiological characteristics of Kp in asymptomatic human carriage, and attached risk factors including environmental sources exposure.MethodsHere, 911 pregnant women from communities in Madagascar, Cambodia, and Senegal were screened for gut colonization by Kp. Characteristics of Kp strains (antimicrobial susceptibility, genomic diversity, virulence, and resistance genes) were defined, and associated risk factors were investigated.ResultsKp carriage rate was 55.9%, and Kp populations were highly heterogeneous (6 phylogroups, 325 sequence types, Simpson index 99.6%). One third of Kp isolates had acquired antimicrobial resistance genes. MDR-Kp (11.7% to 39.7%) and extended spectrum beta-lactamase (ESBL)-producing Kp (0.7% to 14.7%) varied among countries. Isolates with virulence genes were detected (14.5%). Environmental exposure factors including food, animal contacts, or hospitalization of household members were associated with carriage of Kp, antimicrobial resistance and hypervirulence. However, risk factors were country-specific and Kp subpopulation-specific.ConclusionThis large-scale multicenter study uncovers the huge diversity of Kp in human gut carriage, demonstrates that antimicrobial resistance is widespread in communities of three low-income countries, and underlines the challenges posed by Kp colonization to the control of antimicrobial resistance.
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- 2020
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50. Meat and Fish as Sources of Extended-Spectrum β-Lactamase-Producing Escherichia coli, Cambodia.
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Nadimpalli, Maya, Vuthy, Yith, de Lauzanne, Agathe, Fabre, Laetitia, Criscuolo, Alexis, Gouali, Malika, Bich-Tram Huynh, Naas, Thierry, Phe, Thong, Borand, Laurence, Jacobs, Jan, Kerléguer, Alexandra, Piola, Patrice, Guillemot, Didier, Le Hello, Simon, Delarocque-Astagneau, Elisabeth, Huynh, Bich-Tram, and BIRDY study group
- Subjects
ESCHERICHIA coli ,BETA lactamases ,PUBLIC health ,AMIDASES ,HUMAN services - Abstract
We compared extended-spectrum β-lactamase-producing Escherichia coli isolates from meat and fish, gut-colonized women, and infected patients in Cambodia. Nearly half of isolates from women were phylogenetically related to food-origin isolates; a subset had identical multilocus sequence types, extended-spectrum β-lactamase types, and antimicrobial resistance patterns. Eating sun-dried poultry may be an exposure route. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
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