Search

Background: Cardiac arrest remains a critical global health challenge, necessitating effective interventions to improve outcomes. Autopulse Mechanical Chest Compression has emerged as a potential adjunct to conventional manual cardiopulmonary resuscitation (CPR). This study aimed to comprehensively investigate the association between Autopulse use and various outcomes following cardiac arrest. Methods: A thorough literature review was conducted using PubMed, Embase, Scopus, Google Scholar, CINAHL, and the Cochrane Library. Articles were evaluated for eligibility according to predefined criteria. The collected data was synthesized, and the findings were subsequently presented. Results: Sixteen studies were included in the study. A synthesis of diverse studies revealed nuanced perspectives on Autopulse Mechanical Chest Compression. Enhanced CPR success and improved survival rates in out-of-hospital cardiac arrest were demonstrated, while potential drawbacks were suggested, warranting scrutiny of device design and implementation strategies. Meta-analyses on ROSC favored Autopulse, indicating a statistically significant advantage over Manual CPR (relative risk 1.43, 95% CI 1.07-1.92). Complication analyses showed no significant difference (relative risk 1.11, 95% CI 0.93-1.33), emphasizing comparable safety profiles. Patient survival rates exhibited variations, with Autopulse demonstrating higher rates in some studies, but nuances were observed in 30-day survival and hospital discharge rates. Conclusion: Autopulse Mechanical Chest Compression holds promise as a supplementary strategy in cardiac arrest resuscitation. However, complexities arose in longer-term outcomes, highlighting the need for contextual considerations. [SJEMed 2024; 5(1.000): 030-041]

In this paper, the problem of oscillating solutions for an impulsive hematopoiesis model with positive and negative coefficients is investigated. There are several evolutionary processes, which frequently encounter dramatic shifts at specific times and are sensitive to short-term perturbations. As a result, we construct several oscillation criteria that are either brand-new or enhance many of recent findings in the literature. We also give illustrations of how impulsiveness affects the oscillating solutions of the hematopoiesis model.

Abstract Antimicrobial resistance is a worldwide health problem that demands alternative antibacterial strategies. Modified nano-composites can be an effective strategy as compared to traditional medicine. The current study was designed to develop a biocompatible nano-drug delivery system with increased efficacy of current therapeutics for biomedical applications. Zinc oxide nanoparticles (ZnO-NPs) were synthesized by chemical and green methods by mediating with Moringa olifera root extract. The ZnO–NPs were further modified by drug conjugation and coating with PEG (CIP-PEG-ZnO-NPs) to enhance their therapeutic potential. PEGylated ZnO-ciprofloxacin nano-conjugates were characterized by Fourier Transform Infrared spectroscopy, X-ray diffractometry, and Scanning Electron Microscopy. During antibacterial screenings chemically and green synthesized CIP-PEG-ZnO-NPs revealed significant activity against clinically isolated Gram-positive and Gram-negative bacterial strains. The sustainable and prolonged release of antibiotics was noted from the CIP–PEG conjugated ZnO-NPs. The synthesized nanoparticles were found compatible with RBCs and Baby hamster kidney cell lines (BHK21) during hemolytic and MTT assays respectively. Based on initial findings a broad-spectrum nano-material was developed and tested for biomedical applications that eradicated Staphylococcus aureus from the infectious site and showed wound-healing effects during in vivo applications. ZnO-based nano-drug carrier can offer targeted drug delivery, and improved drug stability and efficacy resulting in better drug penetration.

Abstract Neurons and their connecting axons gradually degenerate in neurodegenerative diseases (NDs), leading to dysfunctionality of the neuronal cells and eventually their death. Drug delivery for the treatment of effected nervous system is notoriously complicated because of the presence of natural barriers, i.e., the blood-brain barrier and the blood cerebrospinal fluid barrier. Palliative care is currently the standard care for many diseases. Therefore, treatment programs that target the disease’s origin rather than its symptoms are recommended. Nanotechnology-based drug delivery platforms offer an innovative way to circumvent these obstacles and deliver medications directly to the central nervous system, thereby enabling treatment of several common neurological problems, i.e., Alzheimer’s, Parkinson’s, Huntington’s, and amyotrophic lateral sclerosis. Interestingly, the combination of nanomedicine and gene therapy enables targeting of selective mutant genes responsible for the progression of NDs, which may provide a much-needed boost in the struggle against these diseases. Herein, we discussed various central nervous system delivery obstacles, followed by a detailed insight into the recently developed techniques to restore neurological function via the differentiation of neural stem cells. Moreover, a comprehensive background on the role of nanomedicine in controlling neurogenesis via differentiation of neural stem cells is explained. Additionally, numerous phytoconstituents with their neuroprotective properties and molecular targets in the identification and management of NDs are also deliberated. Furthermore, a detailed insight of the ongoing clinical trials and currently marketed products for the treatment of NDs is provided in this manuscript. Graphical abstract

Abstract Burn wound treatment remains a significant issue in wound care management especially when multidrug resistant bacterial infection and accumulation are present. Delayed wound healing is mostly due to ineffectiveness of commercially available wound dressings that protects the wound but less efficient in healing perspective. Therefore, nano-based wound dressing might be efficient solution for wound healing management. The present study reports the fabrication and evaluation of zinc oxide (ZnO) or silver nanoparticles (Ag NPs) capped with vitamin A or E nanocomposite that were incorporated in wheat gluten (WG) films. The chemical structure, phase purity, and morphological features confirmed the successful coating of NPs by vitamins A and E and their interaction with WG during film casting. The maximum swelling response was observed by NPs vitamin composite WG films than control films while slow release of vitamins and NPs from films was observed up to 24 h. WG films either carrying ZnO or Ag NPs, and vitamin A or E demonstrated significant antioxidant and antibacterial potential. The NPs-vitamin composite loaded WG films showed wound contraction within 14 days during in vivo burn wound healing experiments on mice model. The rates of wound healing, re-epithelialization, collagen deposition with fibroblast regeneration, adipocytes, and hair follicle development were observed through visual and histopathological examination. The study reveals that vitamin A or E doped ZnO or Ag NPs fabricated in WG can be efficiently used against burn wounds due to their physiochemical and biological properties. Furthermore the biocompatible nature and biodegradable potential make the films more prone to mankind maneuver for initial protection and healing remedy.

Aim: This meta-analysis and systematic review aims to investigate and compare the efficacy of mannitol and hypertonic saline in treating patients who have suffered from traumatic brain injury (TBI). Methods: We reviewed publications from the Medline database, Web of Science, and Google Scholar from inception to 13 October 2022 with only English-based literature. The risk of bias from included Randomized controlled trial (RCT) s was assessed using the Cochrane Collaboration's Tool. Newcastle-Ottawa Scale was employed to assess the included cohort's quality. The main outcomes of this review were treatment failure, mortality, intracranial pressure (ICP) reduction, and Cerebral Perfusion Pressure (CPP) increment. All the statistical analyses were performed using the Review Manager 5.4.1. A random-effects model was used to pool the studies when heterogeneity was seen, and the results were reported in the odds ratios (OR) and mean differences (MD) and the corresponding 95% confidence intervals. Results: The results of the statistical study found that there was a significantly lower treatment failure rate [OR = 1.80 (1.26, 2.57); p = 0.001; I 2 = 74%], the lower mortality rate [OR = 2.17 (1.26, 3.73); p = 0.005; I 2 = 0%], a greater increase in CPP [MD = -1.28 (-2.50, -0.05); p = 0.04; I 2 = 0%] associated with hypertonic saline treatment as compared to mannitol treatment. However, concerning ICP decrease, there was no significant difference between the two treatment options [MD = 1.86 (-0.64, 4.36); p = 0.14; I 2 = 70%]. Conclusions: Quantitative and qualitative analysis of trials and observational studies comparing the efficacy of hypertonic saline and mannitol demonstrates that hypertonic saline is a preferable treatment option with greater effectiveness and safety than mannitol in managing patients with TBI. [SJEMed 2023; 4(1.000): 051-059]

The small carpenter bee, Ceratina smaragdula Fibricius is a non-Apis pollinator of many economically important crops. The nesting biology, nesting preferences, architects, foraging, hibernation, and mating behaviour of this solitary bee were examined in this study. Fifteen nests of C. smaragdula were collected and examined for the targeted attributes. C. smaragdula highly preferred (>80%) the area of field sides in Ravenna grass (Saccharum ravennae) for making their nests, at a preferred height of 82.74 cm from the soil surface. The nesting of C. smaragdula along roadsides and houses was 30-60% and

In developing countries, it is imperative to implement cost-effective strategies for animal humoral response development in the production of antiserum. This study compared the effect of immunization regimens on the humoral immune response of New Zealand White (NZW) rabbits (N = 24) using cell culture rabies vaccine (CCRV) through intradermal (ID) and traditional intramuscular (IM) routes. The rabbits were divided into three experimental groups: (a) IPC-R2 with a two-site one-week regimen; (b) TRC-R3 with a two-site twenty-eight-day regimen; and (c) Alternate-R4 with a four-site one-week regimen. These regimens were then compared to the standard IM schedule of five doses of rabies vaccine administered at days 0, 3, 7, 14, and 28 in control group R-1. The results were evaluated at days 14 and 35 postvaccination using rabies-specific Platelia II™ ELISA kit method. The results showed a better response to the ID regimen than the IM route regarding immunogenicity and volume consumption of the vaccine. The three selected ID regimes showed significantly higher mean titer values than the control IM regimen group R-1 (p

The red palm weevil Rhynchophorus ferrugineus (Olivier, 1790), which belongs to the family Curculionidae, order Coleoptera, is one of the most important palm pests, which leads to heavy losses in date palms. In the last few years, it has been recorded in Iraq, from Safwan city south of Iraq, where it was detected on date palm trees (Phoenix dactylifera L, Arecales: Arecaceae). In the current study, specimens of adult weevils were collected from infested date palms (Phoenix dactylifera), that showed signs of infection in Safwan district, south of Basra province. The results of the investigation showed the presence of phoretic mites associated with the red palm weevil Rhynchophorus ferrugineus (Olivier, 1790). Two genera of Uropodid mites, deutonymph, were attached by anal pedicel to body parts of adult red palm weevils. They were collected and identified as Centrouropoda (Uropodidae), and Uroovbovella (Urodinychidae), according to accurate taxonomic keys. The differentiation between two genera of mites was studied. The deutonymphs were examined and photographed by an AM Scop camera installed on a light microscope, and the insects were photographed by a LEICA microscope. The infestation symptoms were illustrated with pictures.

Waste materials are harmful to the environment when incinerated, dumped in open water, or landfilled. Present-day society faces a serious challenge from the growing amount of waste materials and the need for a sustainable solution. Due to the limited amount of natural raw materials, there is a global need to minimize the amount of waste. Using suitable waste materials, such as waste plastic, as additives in asphalt mixtures is a viable strategy, offering an alternative to virgin materials like polymer-modified asphalt binders. This research studied the influence of waste fibers as a stabilizer and recycled Polyethylene waste as an asphalt modifier in the Stone Mastic Asphalt mix (SMA). Two categories of the SMA mixes were produced, base and modified mixes, with three different mixes under each category. The rheological properties of the Recycled Polyethylene Modified Asphalt (RPMA) have been assessed using different Recycled Polyethylene (RP) dosages at the upper-performance temperature. In addition, two Recycled Fibers (RF), cellulose and jute, were used as stabilizing materials for SMA mixes. The impact of RP and RF on the SMA mixes was investigated using three tests: Drain-down resistance, moisture sensitivity, and rutting performance. Statistical analysis was conducted to assess the effect of the additives on the measured SMA properties. The results showed that adding RP and RF improved the SMA mixes' ability to hold the asphalt and fine material inside the mix and solved the drain-down problem by 81.43%. In addition, adding RP as an asphalt modifier greatly enhanced the moisture and rutting resistance by 47.5% and 93%, respectively.

Abstract Purpose: Human resource behaviour tends to play a vital role in organizational overall performance. The study incorporates two of the most prominent behaviours of leadership and examine their influence on organizational performance and knowledge management practices through employee involvement.

Poly (methyl-methacrylate) (PMMA) doped organic compound (2-(2,3-dimethylphenyl-amino)-N-Phenyl benzamide) (2-NPb (films are produced on glass substrates with 5 μm thickness. The influence that gamma (γ) radiation has on the structure of the surface morphology of polymer films, the structure dependence of the optoelectronic properties and the optical properties of a prepared 2-NPb:PMMA film was studied under different doses (21, 42 and 64 KGy) of γ- rays emitted by a 137Cs source with an exposure rate of 0.56 Gy/min. The surface morphology of the 2-NPb:PMMA film for un-irradiated and irradiated sample was studied using a microscope with a digital camera along with texture, waviness and roughenss analysis of the entire surface of the films. The optical properties, together with an in-depth analysis of the dispersion energies and parameters, optoelectrical characteristics, and certain nonlinear optical parameters were comprehensively examined and addressed. In addition, the study of optical properties also included some nonlinear optical parameters. Results show that by doping organic compound (2-NPb) in PMMA, the absorption spectra of samples are decreased and superficial morphology of the film has been affected by γ-rays. As the polymer film was irradiated with a continuous-solid state laser (Solid-State-Laser −532–100 T) beam of 532 nm, self-diffraction ring patterns were seen, and it was shown that the diffraction ring patterns rely heavily on ring numbers and radiation dosage. The nonlinear refractive index,n2, for polymer film for un-irradiated and irradiated are estimated to be 0.23×10-6, 0.92×10-6, 1.64×10-6, 3.14×10-6 cm2/W, respectively due to diffraction ring patterns. The diffraction ring patterns that were acquired via experimentation are then numerically estimated by making use of the Fresnel-Kirchhoff diffraction integral. The results of these calculations have satisfactory qualitative and quantitative accords.

The proboscis extension response (PER) assay revealed the responsiveness of three subspecies of the honeybee Apis mellifera [A. m. jemenitica (AMJ), A. m. carnica (AMC), and A. m. ligustica (AML)] to water and different concentrations (0.00001, 0.0001, 0.001, 0.01, 0.1, 0.5, 1.0, and 1.5 M) of three sugars (fructose, glucose, and sucrose) during the summer and fall seasons. The tested bee subspecies showed significantly different PERs to sugar types across the seasons. The water responsiveness of AMJ, a native bee subspecies, was significantly lower than that of AMC and AML, which showed an equally higher water response in both seasons. During the summer season, AMJ and AMC were equally responsive to each sugar type at all tested concentrations. AML was relatively less responsive to glucose at 0.001, 0.001, 0.01, 0.1, 0.5, and 1.0 M than to fructose and sucrose during the summer season. During the fall season, AMJ was equally responsive to glucose and sucrose at all tested concentrations but showed a significantly different response between fructose and sucrose at 0.001, 0.01, 0.1, 0.5, and 1.0 M concentrations. The PER of AMJ to fructose was lower than that of glucose and sucrose. AMC was equally responsive to all tested sugars at all concentrations, and AML showed a differential response between glucose and sucrose at different concentrations during the fall season. The inter-specific species comparisons revealed that all tested subspecies were equally responsive to fructose at all tested concentrations, and AMJ was more responsive to glucose and sucrose than AMC and AML during both seasons. AMC and AML showed no differences in PER to glucose and sucrose in either season. The AMJ, AMC, and AML nectar and pollen foragers showed no significant differences in PER to glucose and sucrose. The AMC nectar foragers were highly responsive to sucrose than pollen foragers at higher sucrose concentrations (0.1, 0.5, 1.0, and 1.5 M). The AML (nectar forager vs. pollen forgers) showed identical PER to sucrose and glucose but a higher response of nectar foragers to high glucose concentrations (0.5, 1.0, and 1.5 M) than pollen foragers. For water responsiveness, AMJ nectar and pollen foragers showed similar PER to water, whereas AMC and AML pollen foragers were significantly more responsive to water than nectar foragers.

We investigated the effect of green tea extract PEGylated gold nanoparticles (P-AuNPs) making use of its targeted and sustained drug delivery against cyclophosphamide (CYP)-induced cystitis. AuNPs were synthesized by reduction reaction of gold salts with green tea extract following the concept of green synthesis. Mostly spherical-shaped P-AuNPs were synthesized with an average size of 14.3 ± 3.3 nm. Pre-treatment with P-AuNPs (1, 10 mg/kg, i.p.) before CYP (150 mg/kg, i.p.) challenge suggested its uroprotective properties. P-AuNPs significantly reversed all pain-like behaviours and toxicities produced by CYP resulting in a decreased aspartate aminotransferase, alanine aminotransferase, C-reactive protein, and creatinine level. P-AuNPs increased anti-oxidant system by increasing the level of reduced glutathione, glutathione-S-transferase, catalase and superoxide dismutase, and reduced nitric oxide production in bladder tissue. Additionally, it attenuated hypokalaemia and hyponatremia, along with a decrease in Evans blue content in bladder tissue and peritoneal cavity. CYP-induced bladder tissue damage observed by macroscopic and histological findings were remarkably attenuated by P-AuNPs, along with reduced fibrosis of collagen fibre in bladder smooth muscles shown by Masson’s trichrome staining. Additionally, alterations in hematological parameters and clinical scoring were also prevented by P-AuNPs suggesting its uroprotective effect.

Abstract Background Liver diseases post-COVID-19 vaccination is extremely rare but can occur. A growing body of evidence has indicated that portal vein thrombosis, autoimmune hepatitis, raised liver enzymes and liver injuries, etc., may be potential consequence of COVID-19 vaccines. Objectives To describe the results of a systematic review for new-onset and relapsed liver disease following COVID-19 vaccination. Methods For this systematic review, we searched Proquest, Medline, Embase, PubMed, CINAHL, Wiley online library, Scopus and Nature through the Preferred Reporting Items for Systematic Reviews and Meta Analyses PRISMA guideline for studies on the incidence of new onset or relapsed liver diseases post-COVID-19 vaccination, published from December 1, 2020 to July 31, 2022, with English language restriction. Results Two hundred seventy-five cases from one hundred and eighteen articles were included in the qualitative synthesis of this systematic review. Autoimmune hepatitis (138 cases) was the most frequent pathology observed post-COVID-19 vaccination, followed by portal vein thrombosis (52 cases), raised liver enzymes (26 cases) and liver injury (21 cases). Other cases include splanchnic vein thrombosis, acute cellular rejection of the liver, jaundice, hepatomegaly, acute hepatic failure and hepatic porphyria. Mortality was reported in any of the included cases for acute hepatic failure (n = 4, 50%), portal vein thrombosis (n = 25, 48.1%), splanchnic vein thrombosis (n = 6, 42.8%), jaundice (n = 1, 12.5%), raised liver enzymes (n = 2, 7.7%), and autoimmune hepatitis (n = 3, 2.2%). Most patients were easily treated without any serious complications, recovered and did not require long-term hepatic therapy. Conclusion Reported evidence of liver diseases post-COIVD-19 vaccination should not discourage vaccination against this worldwide pandemic. The number of reported cases is relatively very small in relation to the hundreds of millions of vaccinations that have occurred and the protective benefits offered by COVID-19 vaccination far outweigh the risks.

The aim of this study was to test the causal relationship among fear of Covid-19, loneliness, and depression from Covid-19. The study sample consisted of 605 undergraduate students recruited from a public university. They completed three scales: fear of Covid-19, depression from Covid-19, and loneliness. The results revealed a good fit index for the proposed path model. The fear of Covid-19 predicted two types of loneliness emotional and social negatively. A positive association between depression and emotional as well as social loneliness was found. A total of 43% of the variance in depression was explained by loneliness. Depression was potentially a mediating factor between a fear of pandemic and the feeling of loneliness. A counseling intervention can be designed based on these results.

Ulcerative colitis (UC) is a type of inflammatory bowel disease characterized by inflammation, ulcers and irritation of the mucosal lining. Oral drug delivery in UC encounters challenges because of multifaceted barriers. Dexamethasone-loaded galactosylated-PLGA/Eudragit S100/pullulan nanocargoes (Dexa-GP/ES/Pu NCs) have been developed with a dual stimuli-sensitive coating responsive to both colonic pH and microbiota, and an underneath galactosylated-PLGA core (GP). The galactose ligand of the GP preferentially binds to the macrophage galactose type-lectin-C (MGL-2) surface receptor. Therefore, both stimuli and ligand-mediated targeting facilitate nanocargoes to deliver Dexa specifically to the colon with enhanced macrophage uptake. Modified emulsion method coupled with a solvent evaporation coating technique was employed to prepare Dexa-GP/ES/Pu NCs. The nanocargoes were tested using in vitro, ex vivo techniques and dextran sodium sulfate (DSS) induced UC model. Prepared nanocargoes had desired physicochemical properties, drug release, cell uptake and cellular viability. Investigations using a DSS-colitis model showed high localization and mitigation of colitis with downregulation of NF-ĸB and COX-2, and restoration of clinical, histopathological, biochemical indices, antioxidant balance, microbial alterations, FTIR spectra, and epithelial junctions’ integrity. Thus, Dexa-GP/ES/Pu NCs found to be biocompatible nanocargoes capable of delivering drugs to the inflamed colon with unique targeting properties for prolonged duration.

The solar photocatalytic degradation of diuron, which is one of the herbicides, has been studied by a solar pilot plant in heterogeneous solar photocatalysis with titanium dioxide. The pilot plant was made up of compound parabolic collectors specially designed for solar photocatalytic applications. The influence of different variables such as, H2O2 initial concentration, TiO2 initial concentration, and diuron initial concentration with their relationship to the degradation efficiency were studied. Hydrogen peroxide (H2O2) found to increase the rate of diuron degradation. The best removal efficiency of heterogeneous solar photocatalytic TiO2 system was found to be 46.65 % and for heterogeneous solar photocatalytic TiO2/ H2O2 system was found to be 80.65 %. Based on these results, the solar photocatalytic degradation by TiO2/ H2O2 system could be a useful technology for the treatment of effluents containing diuron.

Each year, millions of tons of household and industrial waste, including plastic, are responsible for harming the environment through burning, landfilling, or going to dump in open water. Waste plastic disposal is a critical issue faced by municipalities in modern cities. In addition, the development of hundreds of kilometers of roads worldwide leads to the consumption of vast quantities of raw materials and the depletion of valuable natural resources. The high expense of virgin polymers and rising plastic waste have shifted the research focus toward green pavements that utilize waste plastics. Therefore, this research aims to investigate the use of recycled plastic waste as a binder modifier for Stone Mastic Asphalt mix (SMA). The high-temperature performance of the Recycled Polyethylene Modified Binder (RPMB) has been evaluated with various percentage dosages to satisfy the environment of the Gulf region. In addition, the impact of RPMB on the SMA mix was studied through three performance tests, drain down resistance, moisture damage resistance, and rutting performance. The results showed continuous improvement in rheological properties with increased recycled polyethylene (RP) content by up to 6%. The RP enhances the binder properties such as the rutting parameter, non-recoverable compliance, and viscosity to meet the high-temperature performance requirement for heavy traffic (UPG 76 H) of most roads in the gulf environment. However, the results showed that RPMB could not meet the recovery requirement set by AASHTO TP 70. That is due to RP's plastomer nature. Therefore, it is necessary to supplement some proportion of the RP with an elastomeric polymer. Further, the results of SMA mix properties with RPMB showed remarkable improvement over than control mixture in terms of resistance to drain down, moisture damage, and rutting by 55.1%, 52.6%, and 96.4, respectively.

Reservoir fluids properties are very important in reservoir engineering computations such as material balance calculations, well testing analyses, reserve estimates, and numerical reservoir simulations. Isothermal oil compressibility is required in fluid flow problems, extension of fluid properties from values at the bubble point pressure to higher pressures of interest and in material balance calculations (Ramey, Spivey, and McCain). Isothermal oil compressibility is a measure of the fractional change in volume as pressure is changed at constant temperature (McCain). The most accurate method for determining the Isothermal oil compressibility is a laboratory PVT analysis; however, the evaluation of exploratory wells often require an estimate of the fluid behavior prior to obtaining a representative reservoir sample. Also, experimental data is often unavailable.Empirical correlations are often used for these purposes. This paper developed a new mathematical model for calculating undersaturated oil compressibility using 129 experimentally obtained data points from the PVT analyses of 52 bottom hole fluid samples from Mishrif reservoirs in the southern Iraqi oil fields. The new undersaturated oil compressibility correlation developed using Statistical Analysis System (SAS) by applying nonlinear multiple regression method. It was found that the new correlation estimates undersaturated oil compressibility of Mishrif reservoir crudes in the southern Iraqi oil fields much better than the published ones. The average absolute relative error for the developed correlation is 7.16%.

This study examines the vibrations produced by hydropower operations to improve embankment dam safety. This study consists of two parts: In the first part, ANSYS-CFX was used to generate a three-dimensional (3-D) finite volume (FV) model to simulate a vertical Francis turbine unit in the Mosul hydropower plant. The pressure pattern result of the turbine model was transformed into the dam body to show how the turbine unit's operation affects the dam's stability. The upstream reservoir conditions, various flow rates, and fully open inlet gates were considered. In the second part of this study, a 3-D FE Mosul dam model was simulated using an ANSYS program. The operational turbine model's water pressure pattern is conveyed to the dam's turbine-shared zone. The maximal and minimal upstream reservoir water levels were investigated. A control program was created depending on the principle stress model results collected from the operation of the hydropower plant with the minimum principal stress on the dam body. This research suggests an enhanced turbine operating system, reducing stress on the dam body and increasing dam operation life.

The design of an experimental approach, the Box–Behnken design, was implemented to optimize the chromatographic condition to develop a rapid HPLC procedure for quantification of a ternary mixture of metoprolol (MET), telmisartan (TEL), and amlodipine (AML) from the formulation. The perturbation plots, contour, and 3D response surface pictures were developed to study the impact of each variable on the analytes’ retention time and the probable interaction between the parameters with fewer chromatographic runs. The optimized HPLC method separated the three analytes within 5 min with excellent selectivity and peak shape on a Zorbax C18 HPLC column using acetonitrile and phosphate buffer (20 mM, pH 5.8) with isocratic elution at a 1.1 mL/min flowrate. A wavelength 230 nm was utilized to monitor the elute. The validation of proposed method demonstrated a wide linearity range of 10–200 µg/mL for MET and TEL and 5–50 µg/mL for AML along with an excellent correlation coefficient. The correctness of the HPLC approach was further confirmed by excellent recovery of the added amount of analytes utilizing the standard addition technique. The recommended HPLC approach was employed safely for quality assurance of the formulation, because the evaluation of the method’s greenness and whiteness confirmed the environmentally friendly nature of the approach.

Heavy metals create serious health problems, so the practical implementation and development of low-cost sorbent materials to remove heavy metals from the ecosystem is a worldwide issue. The purpose of this study is to find a low-cost ligand that has the potential to adsorb heavy metals from aqueous and soil samples and also has biological potential. For this, a Schiff base, dimeric o-toluidine (SBL), has been synthesized through condensation, characterized by spectroscopic analysis, and had its biological activities measured. We also studied its adsorption efficiency through a batch technique to remove Zn(II), Co(II), and Cu(II) from aqueous and soil samples under different conditions such as metal ion concentration, pH, contact time, and SBL concentration. The adsorption potential of SBL was analyzed by the Langmuir and Freundlich adsorption isotherms. The values of correlation coefficients revealed that the Freundlich isotherm elucidated results that were more appropriable than the Langmuir model. Adsorption equilibrium was established in 90 min for aqueous samples and in 1,440 min for soil samples. For the maximum adsorption of all metals, the optimum pH was 8, and it showed a capacity to remove 77 to 95 percent of metals from the samples. The maximum adsorption capacity (qmax) of SBL were 75.75, 62.50, and 9.17 mg g-1 in the case of Cu(II), Zn(II), and Co(II) ions, respectively, from aqueous samples and 10.95, 64.10, and 88.49 mg g-1 in the case of Zn(II), Cu (II), and Co(II), respectively, from soil samples. The effectiveness of SBL in the sorption of the selected metals was found to be Cu+2 > Zn+2 > Co+2 for aqueous samples and Co+2 > Cu+2 > Zn+2 for soil samples. The antimicrobial activity of SBL was also investigated. The results revealed that SBL showed moderate inhibitory activity against Staphylococcus dysentria, C. albican, and Aspergillus niger, whereas it exhibited weak activity against S. aureus, P. aureginosa, K. pneumoniae, P. vulgaris, and E.coli when compared to Fluconazole and Ciprofloxacin as the standard. Acute toxicity of the synthesized compound was measured through its daily oral administration with various doses ranging from 0.1 to 1,000 mg/kg of the mice’s body weights. Even at the dose of 1,000 mg/kg, the SBL showed no mortality or any type of general behavioral change in the treated mice. Based on preparation cost, metal removal capacity, toxicity, and antimicrobial activities, SBL is an excellent sorbent and should be studied at pilot scale levels.

The snow leopard is one of the highly valued species from high-altitude mountain ecosystems of Central and Southeast Asia, including Pakistan. This keystone species is facing a myriad of conventional and emerging threats, including poaching and trade, that are poorly documented in Pakistan. To understand the dynamics and drivers of the poaching and trading of snow leopards in Pakistan, we investigated the issue in depth through a multifaceted survey in the snow leopard range of the country. We recorded 101 snow leopard poaching incidences from 11 districts during 2005–2017. The reported poaching incidences varied spatially (x̄ = 9 ± 2.6 [95% Cl: 3–15]) and temporally (x̄ = 7.8 ± 1.09) and accounted for 2–4% annual population loss (n = 200–420) in a period of 13 years. Poaching and trade together constituted 89% of the total incidence reported and animals were mostly shot (66%), poisoned (12%), snared (12%) and captured (4%), respectively. Only a fraction (3%) of the incidences were reported to the relevant law enforcement agencies. Trade routes included large cities and neighbouring countries, even the Middle East and Europe. The average base and end prices for each item were 245 ± 36 USD and 1,736 ± 520 USD, respectively, while maximum monetary fines set as per the law were 275 USD. Our results establish the need for developing multi-stakeholder coordination mechanisms at regional, national and international levels and information sharing to curb this menace. Improving the existing laws and surveillance system, while taking the local communities onboard, will further help to this end.

In this paper, oscillation and asymptotic behavior of three-dimensional third-order delay systems are discussed. Some sufficient conditions are obtained to ensure that every solution of the system is either oscillatory or nonoscillatory and converges to zero or diverges as t goes to infinity. A special technique is adopted to include all possible cases for all nonoscillatory solutions (NOSs). The obtained results included illustrative examples.

This prospective quasi-experimental study from the NASAM (National Approach to Standardize and Improve Mechanical Ventilation) collaborative assessed the impact of evidence-based practices including subglottic suctioning, daily assessment for spontaneous awakening trial (SAT), spontaneous breathing trial (SBT), head of bed elevation, and avoidance of neuromuscular blockers unless otherwise indicated. The study outcomes included VAE (primary) and intensive care unit (ICU) mortality. Changes in daily care process measures and outcomes were evaluated using repeated measures mixed modeling. The results were reported as incident rate ratio (IRR) for each additional month with 95% confidence interval (CI). A comprehensive program that included education on evidence-based practices for optimal care of mechanically ventilated patients with real-time benchmarking of daily care process measures to drive improvement in forty-two ICUs from 26 hospitals in Saudi Arabia (>27,000 days of observation). Compliance with subglottic suctioning, SAT and SBT increased monthly during the project by 3.5%, 2.1% and 1.9%, respectively (IRR 1.035, 95%CI 1.007-1.064, p = 0.0148; 1.021, 95% CI 1.010-1.032, p = 0.0003; and 1.019, 95%CI 1.009-1.029, p = 0.0001, respectively). The use of neuromuscular blockers decreased monthly by 2.5% (IRR 0.975, 95%CI 0.953-0.998, p = 0.0341). The compliance with head of bed elevation was high at baseline and did not change over time. Based on data for 83153 ventilator days, VAE rate was 15.2/1000 ventilator day (95%CI 12.6-18.1) at baseline and did not change during the project (IRR 1.019, 95%CI 0.985-1.053, p = 0.2812). Based on data for 8523 patients; the mortality was 30.4% (95%CI 27.4-33.6) at baseline, and decreased monthly during the project by 1.6% (IRR 0.984, 95%CI 0.973-0.996, p = 0.0067). A national quality improvement collaborative was associated with improvements in daily care processes. These changes were associated with a reduction in mortality but not VAEs. Registration The study is registered in clinicaltrials.gov (NCT03790150).

This study investigates the impact of corporate social responsibility (CSR) on firm reputation and organizational citizenship behavior, along with the mediating inclusion of organic organizational cultures (Clan and Adhocracy) in the medium and large enterprises of Pakistan. To do the path analysis and to investigate the mediating role of organic organization culture, Smart PLS was used. For data collection, the convenience sampling technique was used and responses from 360 questionnaires were the main data source. The results displayed that CSR has a significant and optimistic effect on a firm reputation and employees’ organizational citizenship behavior (OCB). Secondly, the outcomes showed a positive and full mediation effect of organic organizational cultures between CSR and firm reputation but a partial mediation effect of organic organizational cultures between CSR and employees OCB. Further, the results demonstrated a positive and significant influence of employees’ OCB on a firm reputation. The extensive analysis of all factors of the study was autonomously examined to identify the insights that verify how the inclusion of organic organizational cultures can boost the firm reputation and employees’ OCB. Theoretical implications and future research direction are discussed.

Mountain ungulates perform a key role in maintaining the balance of ecosystems as they are the primary consumers of vegetation and prey for large predators. The mountain ranges of northern Pakistan are home to six species of mountain ungulates, and the Himalayan ibex (Capra sibirica), hereafter ibex, is the most abundant among them. This study was conducted in three administrative regions of northern Pakistan, viz. Gilgit-Baltistan (GB), Azad Jammu and Kashmir (AJK), and Khyber Pakhtunkhwa (KP), to generate a range-wide density pattern map of ibex. A double-observer survey was conducted in 25 study sites during 2018–2021 across the ibex distribution range, covering an area of about 35,307 km2, by walking transects totaling 1647 km. Within the ibex range where the survey was not conducted due to financial and logistical constraints, we obtained species population information from local wildlife departments’ most recent annual survey data. The aim was to generate a density map for the entire ibex range. Using the BBRecapture package in program R, we estimated an ibex population of 7639 (95 % CI) with a mean density of 0.21/km2 in the surveyed area. Combining with the secondary data from un-surveyed areas, the total population estimate for the country came to 10,242 ibex. The largest population densities were observed in four valleys (Shimshal, Gulkin-Hussaini, Khyber, and Khunjerab) of the Karakoram-Pamir range, followed by the Hindu Kush range (Chitral Wildlife Division [WD]). The central and eastern parts of the Karakoram range had moderate to low densities, while the Himalayan range (e.g., Astore Valley) supported a small population. The mean herd size was 15 individuals (range: 5–41), and the average detection probability of observers A and B was 0.69 and 0.48, respectively. The average male and young ratios per 100 females were estimated to be 75 and 81, respectively. The range-wide density map developed during the study provided an evidence for the impact of trophy hunting programs and an objective tool for range-wide conservation planning of the species.

Objective: To evaluate histologically the deposition of iron in the epiphyseal cartilage of offspring’s of dams given iron supplementation during pregnancy and lactation in rat model. Study Design: Laboratory based experimental study. Place and Duration of Study: Department of Anatomy, Army Medical College, Rawalpindi and National Institute of Health (NIH) Islamabad, from Mar to Nov 2016. Methodology: In this study, 16 female and 4 male adult rats were chosen for breading. After confirmation of pregnancy, pregnant rats were separated in two groups. One group was given oral iron supplementation for four weeks till delivery and half of the pups fed by mothers who were given iron during lactation. The other group was kept on normal lab diet. Deposition of iron in the epiphyseal cartilage of newborn rats after four weeks was evaluated histologically in pups. Results: Iron deposition was maximum in group C i.e. 1.30 ± 0.48; in group B it was 0.20 ± 0.44. Statistically significant iron deposition (p

Alzheimer's disease (AD) is a well-known type of age-related dementia. The present study was conducted to investigate the effect of xanthoangelol against memory deficit and neurodegeneration associated with AD. Preliminarily, xanthoangelol produced neuroprotective effect against H2O2-induced HT-22 cells. Furthermore, effect of xanthoangelol against scopolamine-induced amnesia in mice was determined by intraperitoneally (i.p.) administering xanthoangelol (1, 10 and 20 mg/kg), 30 min prior to induction. Mice were administered scopolamine at a concentration of 1 mg/kg; i.p. for the induction of amnesia associated with AD. Xanthoangelol dose dependently reduced the symptoms of Alzheimer’s disease as observed by the results obtained from the behavioral analysis performed using Morris water maze and Y-maze test. The immunohistochemical analysis suggested that xanthoangelol significantly improved Keap-1/Nrf-2 signaling pathway. It greatly reduced the effects of oxidative stress and showed improvement in the anti-oxidant enzyme such as GSH, GST, SOD and catalase. Additionally, xanthoangelol decreased the expression of transient receptor potential vanilloid 1 (TRPV-1), a nonselective cation channel, involved in synaptic plasticity and memory. It activated the anti-oxidants and attenuated the apoptotic (Bax/Bcl-2) pathway. Xanthoangelol also significantly attenuated the scopolamine-induced neuroinflammation by the inhibition of interleukin-1 beta (IL-1β), and tumor necrosis factor-α (TNF-α) levels. The histological analysis, showed a significant reduction in amyloid plaques by xanthoangelol. Therefore, the present study indicated that xanthoangelol has the ability to ameliorate the AD symptoms by attenuating neuroinflammation and neurodegeneration induced by scopolamine.

In this article, we developed the idea of q-time scale calculus in quantum geometry. It includes the q-time scale integral operators and ∆q-differentials. It analyzes the fundamental principles which follow the calculus of q-time scales compared with the Leibnitz–Newton usual calculus and have few crucial consequences. The ∆q-differential reduced method of transformations was proposed to work out on partial Δq-differential equations in time scale. With easily computable coefficients, the result is calculated in the version of a power series which is convergent. The performance and effectiveness of the proposed procedure are also illustrated, and Matlab software is applied for calculation with the support of some fascinating examples. It changes when σt=t and q = 1; then, the solution merges with usual calculus for the mentioned initial value problem. The finding of the present work is that the Δq-differential transformation reduced method is convenient and efficient.

Healthcare workers in Pakistan are still fighting at the frontline to control the spread of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and have been identified as the earliest beneficiaries for COVID-19 vaccination by the health authorities of the country. Besides, the high vaccination rates of frontline healthcare workers (FHWs) are essential to overcome the ongoing pandemic and reduce the vaccines hesitancy among the general population. The current research employed the theory of planned behavior (TPB) to investigate the COVID-19 vaccination behavior among FHWs in Pakistan as well as the predictors of such behavior. Following the epidemic control and prevention policies, a sample of 680 FHWs were accessed to fill in the questionnaire evaluating the components of the TPB. Moreover, the potential role of anticipated regret (AR) and perceived susceptibility (PS) on COVID-19 vaccination behavior was also assessed. The partial least square structural equation modeling (PLS-SEM) results revealed that the TPB components, as well as the AR, have positive associations with the COVID-19 vaccination behavior. The results further confirmed that PS positively affects the anticipated regret, attitude (ATT), and subjective norm (SN) to vaccinate against SARS-CoV-2. The perceived susceptibility also has a positive association with COVID-19 vaccination behavior through the mediation of anticipated regret, ATT, and SN. Our findings highlighted the importance of COVID-19 vaccination among healthcare workers, which can be applied to reduce vaccine hesitancy among the general public.

Access to finance plays a central pillar on the sustainable firm growth of developing and developed nations. This study depicts the linkage between access to external finance, firm quality, and firms’ performance as measured by labor productivity for sustainable small- and medium-sized enterprises (SMEs’) development by employing the ordinary least squares (OLS) regression and propensity score matching (PSM) techniques to alleviate the selection bias and endogeneity issue on Word bank enterprise survey (WBES) cross-sectional firm-level data of 3,196 Bangladeshi SMEs for 2007–2013 period. Empirical evidence linking access to external finance and labor productivity has been positive and significant. However, our finding explores a negative but significant relationship between exports and SME labor productivity. A further look into the results also exhibits no statistical significance in the interaction effect between firm quality and access to finance on labor productivity. Moreover, the study anticipates novel empirical support that, the disintegration effect of export sales between direct and indirect exports with labor productivity for credit-accessed firms, is also found statistically insignificant. Then, several policies are drawn from the results to gain international competitiveness, and to ensure more external finance channels for enhancing SMEs’ performance and sustainable firm growth.

Abstract Background Diabetes mellitus is a chronic disease characterized by hyperglycemia that may occur due to genetic, environmental or lifestyle factors. Natural remedies have been used to treat diabetes since long and many antidiabetic compounds of varied efficacies have been isolated from medicinal plants. Rhazya stricta has been used for decades for the treatment of diabetes mellitus and associated ailments. Considering the folkloric use of R. stricta against diabetes, it was aimed to investigate the effectiveness of its root extracts against diabetes through in vitro assays and in vivo studies using animal model along with phytochemical profiling through GCMS. Methods Various fractions of Rhazya stricta obtained through column chromatography were evaluated for a variety of assays including α-glucosidase, Dipeptidyl peptidase-IV (DPP-IV), β-secretase and Glucagon-like peptide-1 (GLP-1) secretion studies. For the in vivo studies the alloxan-induced diabetic mice were treated with root extracts and blood glucose levels, HbA1C, and other biochemical markers along with the histological study of the liver were done. The phytochemical identification was performed using an Agilent 7890B GC coupled to a 7010 Triple Quadrupole (MS/MS) system. GraphPad Prism software version 5.01 was used for statistical analysis. Results Majority of the extract fractions showed excellent results against diabetes by inhibiting enzymes DPP-IV (Up to 61%) and β-secretase (Up to 83%) with IC50s 979 μg/ml and 169 μg/ml respectively with increase in the GLP1 secretion. The results of in vivo studies indicated a marked reduction in blood glucose and HbA1c levels along with positive effects on other parameters like lipid profile, liver functions and renal functions of extract-treated mice as compared to control. The histological examination of the liver demonstrated hepatoprotective effects against diabetes led changes and various classes of phytochemicals were also identified through GCMS in different fractions. Conclusion The results revealed strong antidiabetic activity of R. stricta root with the potential to protect body organs against diabetic changes. Moreover, a variety of phytochemicals has also been identified through GCMS that might be responsible for the antidiabetic potential of Rhazya stricta root. Graphical abstract

Abstract Background In the present study, the poncirin which is flavonoid-7-o-glycosides (isolated from the Poncirus trifoliata) in nature was evaluated against the Carbon tetra chloride (CCL4)-induced liver injury. The poncirin have been reported for various anti-inflammatory, analgesic activity etc. Based on the previous studies it was anticipated that the poncirin will ameliorate CCL4-induced liver injury. Methods The CCL4-induced acute and chronic liver injury model (albino BALB/c mice) was used. Following the induction of the liver injury various parameters such as food and water intake, body weight and weight to dry ratio changes were assessed. Furthermore, various hematological, biochemical parameters and histological studies such as hemotoxylin and eosin (H and E) staining were performed. The poncirin treatment was also evaluated against the pro-inflammatory cytokines such as interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) using enzyme link immunosorbant assay (ELISA). The Swiss Target prediction software was used to investigate interaction of the poncirin on the various hepatic enzymes. Results The poncirin treatment markedly improved the behavioral parameters such as food and water intake. The liver weight variation was attenuated and total body was improved markedly. The hematological and biochemical parameters were significantly improved compared to the CCL4 treated groups. The anti-oxidants were induced, while oxidative stress markers were reduced promisingly. The H and E staining showed that poncirin treatment significantly improved the histology of liver compared to the CCL4 treated group. Furthermore, the poncirin treatment also evidently decreased the inflammatory mediators. Conclusions The poncirin treatment showed marked improvement in behavioral, biochemical and histological parameters following CCL4-induced liver injury. Additionally, the poncirin treatment also markedly improved the antioxidant enzymes, attenuated the oxidative stress markers and inflammatory cytokines.

The human intestine harbors a huge number of diverse microorganisms where a variety of complex interactions take place between the microbes as well as the host and gut microbiota. Significant long-term variations in the gut microbiota (dysbiosis) have been associated with a variety of health conditions including inflammatory bowel disease (IBD). Conventional fecal microbiota transplantations (FMTs) have been utilized to treat IBD and have been proved promising. However, various limitations such as transient results, pathogen transfer, storage, and reproducibility render conventional FMT less safe and less sustainable. Defined synthetic microbial communities (SynCom) have been used to dissect the host-microbiota-associated functions using gnotobiotic animals or in vitro cell models. This review focuses on the potential use of SynCom in IBD and its advantages and relative safety over conventional FMT. Additionally, this review reinforces how various technological advances could be combined with SynCom to have a better understanding of the complex microbial interactions in various gut inflammatory diseases including IBD. Some technological advances including the availability of a gut-on-a-chip system, intestinal organoids, ex vivo intestinal cultures, AI-based refining of the microbiome structural and functional data, and multiomic approaches may help in making more practical in vitro models of the human host. Additionally, an increase in the cultured diversity from gut microbiota and the availability of their genomic information would further make the design and utilization of SynCom more feasible. Taken together, the combined use of the available knowledge of the gut microbiota in health and disease and recent technological advances and the development of defined SynCom seem to be a promising, safe, and sustainable alternative to conventional FMT in treating IBD.

Introduction: Fear is the normal response to any perceived threat especially when it comes to contracting a new disease resulting in stress and anxiety. This study was conducted during the peak epidemic phase of COVID-19 in Pakistan to assess fear level among Pakistani population regarding COVID-19. Method: A cross-sectional study was conducted during June-July 2020 in Pakistan. A pre-designed questionnaire based on “Fear of COVID-19 Scale” was distributed through email and social media platforms. Descriptive analysis was conducted and frequencies were calculated. Each response scored as 1 (strongly disagree) to 5 (strongly agree). The fear level was categorized as high (>20 score), Moderate (14-20 score) and low (

The outgrowth of e-commerce has advanced the development of countries' economies. Today, online marketplaces are targeting not only their local customers but are also spreading their interests overseas, expanding cross-border e-commerce. The current study aims to analyze the interaction of customer's personal traits, such as national culture, disposition to trust, and perceived trustworthiness, and their effect on the purchase intention within different e-commerce contexts. The contexts are chosen based on the country-of-origin parameter and serve as the moderator in the research model. Both direct and indirect effects of cultural dimensions on trustworthiness and purchase intention are analyzed within the research framework. The data for the analysis are randomly collected among the Russian population and assessed using structural equation modeling (SEM). The analysis results prove the marketplace context moderates the interaction of customers' personal traits among each other and their effect on the purchase intention. The study shows that dimensions of national culture have a more substantial effect on perceived trustworthiness and purchase intention in the Chinese marketplace context. The current study contributes to the analysis of customer behavior patterns within context, expanding context-related research direction. It increases the specificity of the culture and trustworthiness research and deepens the understanding of country-of-origin moderating effect in e-commerce. Moreover, addressing a high-level uncertainty avoidance culture within the research framework, the study diversifies the existing set of analyzed cultures in the e-commerce environment. The current study is applicable both in domestic and in cross-border e-commerce practice, broadening the understanding of consumer behavior patterns. The research model is relevant for the analysis of trust-effected behavioral outcomes.

This research investigates the impact of transformational leadership (TL) style on project success (PS) in the indirect effect of serial mediation of team-building and teamwork quality (TWQ). A quantitative research approach was used for this study. Data were gathered from 374 professional information system development (ISD) project managers in Pakistan. The hypotheses were tested using regression analysis with bootstrapping. Both team-building and teamwork independently and serially mediate the relationship between the TL and PS of the project managers, respectively. The TL style of the project manager intensifies PS with team-building practices (TBP) and TWQ. The TL boosts TWQ in terms of communication, coordination, and cohesion to achieve a successful project. The findings suggest that TL is associated with PS through serial mediation of team-building and teamwork. No research to date has used this nascent methodology to explore the association between TL and PS.

We surveyed and selectively sampled the major water sources in Bamyan city and the surrounding area to assess the water quality. Water quality measurements were taken in situ and more samples were collected for laboratory analysis from canals, rivers, springs, wells, and water supply systems. In urban areas, water supply systems provide 36% of the drinking water, but in rural areas, this source accounts for only 7% of drinking water supplies. Wells comprise 33% and 15% of urban and rural water supplies, respectively, while canals and rivers are modest water sources for Bamyan communities. Basic water quality parameters, such as pH, EC, and TDS, were variable with high values in some areas. Most of the samples fall in the range of potable water, but some had a high TDS and EC indicating that there is the potential of contamination. Values of pH were mostly were mostly in the range of drinking water (6.5–9.5). A Drinking Water Quality Index (DWQI) was calculated to better understand the water quality issues for the potable water supplies. Subsets of representative samples were analyzed for 17 selected chemical elements and other constituents. Barium (Ba) was detected in almost all of the water samples, while arsenic (As) was detected in about 9% of the analyzed samples, and this was mostly associated with thermal springs. Concentrations of Mn and Cu in some samples exceeded that of the water quality standards, while Zn concentrations were below tolerable limits in all of the samples. Most of the analyzed water samples were hard, and several samples showed evidence of microbial pollution in urban areas. Rivers originating from snow and glacier melting had excellent quality for drinking.

Gilgit-Baltistan (Northern Pakistan) has a rich diversity of wild caprinae and is one of key area for caprinae community-based conservation programs. In this program, selective hunting of small proportion of adult males with large horns is done on annual basis to generate money for the conservation, habitat improvement and livelihood of local communities. The current study aimed at collecting reliable data about population status of Astor Markhor (Capra falconeri falconeri). For this purpose, fixed-point direct count method was used for estimation of Astor Markhor population in 16 catchments including 15 Community-Controlled Hunting Areas (CCHAs). A total of 1087 animals were counted, comprising of 266 (24%) males (including sixty-two (6%) trophy-sized males), 388 (36%) females, 227 (21%) yearling and 206 (19%) kids. Population density of Astor Markhor was estimated 0.13 individuals/km2, with male to female ratio 0.69:1, yearling to female 0.54:1 and kids to female 0.51:1. CCHA/catchment wise assessments showed that Kargah area has highest population (211 animals). It is suggested that consequences of trophy hunting should be strictly contingent upon population data obtained through robust methods, duly verified by a panel of conservation experts and may be extended to other areas also for fruitful results.

Honey production gains are needed to deal with high demand in Saudi Arabia. The honey bee races are facing stressful hot-arid weather conditions that can affect different aspects of physiology and behavior. The hypopharyngeal glands (HPGs) of honey bees have prominent roles in various social behaviors through their secretions. The measurement of acini size and lipofuscin accumulation indicates the changes in HPGs in response to different factors including weather and behavioral castes. This research aimed to reveal how natural harsh environment of summer and winter can shape the HPGs in foragers and nurses of an indigenous bee race (Apis mellifera jemenitica Ruttner) in comparison with two exotic bee races (Apis mellifera carnica Pollmann and Apis mellifera ligustica Spinola). This study presents new information of significant differences in the HPGs of two behavioral castes (nurses and foragers) of indigenous and exotic bee races under harsh natural environmental conditions. HPGs of foragers have significantly higher lipofuscin accumulation and smaller acini size than nurse bees in all tested races during summer and winter seasons. A strong inverse correlation was found between acini size and lipofuscin accumulation in each race in both seasons. Smaller acini size and lipofuscin accumulation were detected in the HPGs of indigenous bees (foragers and nurses) than exotic bee races during both seasons. The acini size and lipofuscin accumulation were similar between exotic bee races but higher than that of the indigenous bee race.

The relative effects of climate warming with grazing on medicinally important plants are not fully understood in Hindukush-Himalaya (HKH) region. Therefore, we combined the indigenous knowledge about culturally important therapeutic plants and climate change with experimental warming (open-top chambers) and manual clipping (simulated grazing effect) and compared the relative difference on aboveground biomass and percent cover of plant species at five alpine meadow sites on an elevation gradient (4696 m-3346 m) from 2016-2018. Experimental warming increased biomass and percent cover throughout the experiment. However, the interactive treatment effect (warming x clipping) was significant on biomass but not on percent cover. These responses were taxa specific. Warming induced an increase of 1 ± 0.6% in Bistorta officinalis percent cover while for Poa alpina it was 18.7 ± 4.9%. Contrastingly, clipping had a marginally significant effect in reducing the biomass and cover of all plant species. Clipping treatment reduced vegetation cover & biomass by 2.3% and 6.26%, respectively, but that was not significant due to the high variability among taxa response at different sites. It was found that clipping decreased the effects of warming in interactive plots. Thus, warming may increase the availability of therapeutic plants for indigenous people while overgrazing would have deteriorating effects locally. The findings of this research illustrate that vegetation sensitivity to warming and overgrazing is likely to affect man-environment relationships, and traditional knowledge on a regional scale.

Climate change is expected to impact a large number of organisms in many ecosystems, including several threatened mammals. A better understanding of climate impacts on species can make conservation efforts more effective. The Himalayan ibex (Capra ibex sibirica) and blue sheep (Pseudois nayaur) are economically important wild ungulates in northern Pakistan because they are sought-after hunting trophies. However, both species are threatened due to several human-induced factors, and these factors are expected to aggravate under changing climate in the High Himalayas. In this study, we investigated populations of ibex and blue sheep in the Pamir-Karakoram mountains in order to (i) update and validate their geographical distributions through empirical data; (ii) understand range shifts under climate change scenarios; and (iii) predict future habitats to aid long-term conservation planning. Presence records of target species were collected through camera trapping and sightings in the field. We constructed Maximum Entropy (MaxEnt) model on presence record and six key climatic variables to predict the current and future distributions of ibex and blue sheep. Two representative concentration pathways (4.5 and 8.5) and two-time projections (2050 and 2070) were used for future range predictions. Our results indicated that ca. 37% and 9% of the total study area (Gilgit-Baltistan) was suitable under current climatic conditions for Himalayan ibex and blue sheep, respectively. Annual mean precipitation was a key determinant of suitable habitat for both ungulate species. Under changing climate scenarios, both species will lose a significant part of their habitats, particularly in the Himalayan and Hindu Kush ranges. The Pamir-Karakoram ranges will serve as climate refugia for both species. This area shall remain focus of future conservation efforts to protect Pakistan’s mountain ungulates.