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425 results on '"Holopainen, Juha M."'

1. TFOS DEWS II Tear Film Report

Author

Willcox, Mark D.P., Argüeso, Pablo, Georgiev, Georgi A., Holopainen, Juha M., Laurie, Gordon W., Millar, Tom J., Papas, Eric B., Rolland, Jannick P., Schmidt, Tannin A., Stahl, Ulrike, Suarez, Tatiana, Subbaraman, Lakshman N., Uçakhan, Omür Ö., and Jones, Lyndon

Published
2017
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11. Severe ocular cowpox in a human, Finland

Author

Kinnunen, Paula M., Holopainen, Juha M., Hemmila, Heidi, Piiparinen, Heli, Sironen, Tarja, Kivela, Tero, Virtanen, Jenni, Niemimaa, Jukka, Nikkari, Simo, Jarvinen, Asko, and Vapalahti, Olli

Subjects
Health
Abstract

To the Editor: We describe cowpox with corneal involvement in a 31-year-old atopic woman who lived in southern Finland and was unvaccinated for smallpox. In August 2009, she noticed irritation [...]

Published
2015

20. Phospholipid transfer protein is present in human tear fluid

Author

Jauhiainen, Matti, Setala, Niko L., Ehnholm, Christian, Metso, Jari, Eriksson, Ove, and Holopainen, Juha M.

Subjects
Esters -- Research, Proteins -- Research, Phospholipids -- Research, Biological sciences, Chemistry
Abstract

An investigation whether phospholipid transfer protein (PLTP) and cholesteryl ester transfer protein (CETP) are present in human tear fluid is conducted, in order to gain insight into the formation of tear film. The results suggest that PLTP may play role in the formation of the tear film by supporting phospholipid transfer.

Published
2005

21. Distribution, orientation, and dynamics of DPH probes in DPPC bilayer

Author

Repakova, Jarmila, Capkova, Pavla, Holopainen, Juha M., Vattulainen, Ilpo, and Katsube, Teruaki

Subjects
Choline -- Structure, Choline -- Atomic properties, Molecular dynamics -- Usage, Chemicals, plastics and rubber industries
Abstract

The distribution, orientation, and dynamics of 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence probes in a dipalmitoylphosphatidylcholine (DPPC) bilayer were studied using 50 ns molecular dynamics. The results indicated that DPH did not prefer to be embedded in the membrane center where free volume was largest.

Published
2004

26. Observation of topical catalysis by Sphingomyelinase coupled to microspheres

Author

Nurminen, Tuula A., Holopainen, Juha M., Hongxia Zhao, and Kinnunen, Paavo K.J.

Subjects
Liposomes -- Properties, Catalysis -- Analysis, Enzymatic analysis, Chemistry
Abstract

A novel approach is described, which studies enzymatic reactions and allows manipulating giant vesicles as well as cultured cells in a spatially controlled manner. The study shows that the enzymatic catalysis can be restrained topically to the site of contact between the vesicle and the derivatized microsphere.

Published
2002

27. Altered expression of matrix metalloproteinases and their tissue inhibitors as possible contributors to corneal droplet formation in climatic droplet keratopathy

Author

Holopainen, Juha M., Serra, Horacio M., Sánchez, María C., Sorsa, Timo, Zalentein, Waldir N., Barcelona, Pablo F., Moilanen, Jukka A.O., Tervahartiala, Taina, Tervo, Timo M.T., Cafaro, Thamara A., Virtanen, Ismo, Urrets-Zavalia, Enrique A., Bhattacharya, Sanjoy K., and Urrets-Zavalia, Julio A.

Published
2011
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32. Sphingomyelinase induces lipid microdomain formation in a fluid phosphatidylcholine/sphingomyelin membrane

Author

Holopainen, Juha M., Subramanian, Marappan, and Kinnunen, Paavo K.J.

Subjects
Sphingolipids -- Research, Lecithin -- Research, Phospholipids -- Research, Biological sciences, Chemistry
Abstract

The physicochemical properties of sphingolipids in a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) matrix were investigated. To this end, the behaviors of two chemically well-defined sphingolipids, N-palmitoyl-sphingomyelin and the corresponding ceramide, were compared. Experimental results showed that the enzymatic generation of ceramide from sphingomyelin stimulates microdomain formation in LUVs, although the reorganization of ceramide into microdomains within the bilayer took a significantly longer time.

Published
1998

38. Activation of phospholipase A(sub 2) by amyloid beta-peptides in vitro

Author

Lehtonen, Jukka Y.A., Holopainen, Juha M., and Kinnunen, Paavo K.J.

Subjects
Alzheimer's disease -- Research, Amyloidosis, Phospholipases -- Development and progression, Biological sciences, Chemistry
Abstract

A synthetic peptide, corresponding to 1 to 42 amino acid residue of amyloid beta-peptide (A-Beta(sub 1-42)), enhances the hydrolysis of the pyrene-labeled acidic phospholipid analog 1-palmitoyl-2-((pyren-1-yl))hexanoyl-sn-glycero-3-phosphoglycerol by porcine pancreatic phospholipase A(sub 2). Ca2+, at higher concentrations, abolishes the enhancing effect of A-Beta(sub 1-42), which is found in senile plaques in Alzheimer's disease (AD). Brain samples of AD patients show decreased levels of phosphatidylcholine. Activation of lipolytic enzyme by amyloid beta peptide indicates a direct involvement of the peptide in neuronal cell death in AD afflicted brain tissue.

Published
1996

44. Relevance of Lipid-Based Products in the Management of Dry Eye Disease

Author

Garrigue, Jean-Sebastien, Amrane, Mourad, Faure, Marie-Odile, Holopainen, Juha M., Tong, Louis, Clinicum, Department of Ophthalmology and Otorhinolaryngology, Silmäklinikka, and HUS Head and Neck Center

Subjects
emulsion, IN-WATER EMULSION, CYCLOSPORINE-A, TEAR FILM, ARTIFICIAL TEARS, OCULAR SURFACE, SODIUM HYALURONATE, eye diseases, MEIBOMIAN GLAND DYSFUNCTION, lipid layer, ocular surface disease, CATIONIC EMULSION, liposome, OPHTHALMIC DRUG-DELIVERY, CASTOR-OIL, sense organs, 3125 Otorhinolaryngology, ophthalmology
Abstract

Components of the ocular surface synergistically contribute to maintaining and protecting a smooth refractive layer to facilitate the optimal transmission of light. At the air-water interface, the tear film lipid layer (TFLL), a mixture of lipids and proteins, plays a key role in tear surface tension and is important for the physiological hydration of the ocular surface and for ocular homeostasis. Alterations in tear fluid rheology, differences in lipid composition, or downregulation of specific tear proteins are found in most types of ocular surface disease, including dry eye disease (DED). Artificial tears have long been a first line of treatment in DED and aim to replace or supplement tears. More recently, lipid-containing eye drops have been developed to more closely mimic the combination of aqueous and lipid layers of the TFLL. Over the last 2 decades, our understanding of the nature and importance of lipids in the tear film in health and disease has increased substantially. The aim of this article is to provide a brief overview of our current understanding of tear film properties and review the effectiveness of lipid-based products in the treatment of DED. Liposome lid sprays, emulsion eye drops, and other lipid-containing formulations are discussed.

Published
2017

46. TFOS DEWS II Tear Film Report

Author

Willcox, Mark D. P., Argueso, Pablo, Georgiev, Georgi A., Holopainen, Juha M., Laurie, Gordon W., Millar, Tom J., Papas, Eric B., Rolland, Jannick P., Schmidt, Tannin A., Stahl, Ulrike, Suarez, Tatiana, Subbaraman, Lakshman N., Ucakhan, Omur O., Jones, Lyndon, Clinicum, Department of Ophthalmology and Otorhinolaryngology, Silmäklinikka, University of Helsinki, and HUS Head and Neck Center

Subjects
OPTICAL COHERENCE TOMOGRAPHY, HUMAN LACRIMAL GLAND, Proteome, BREAK-UP TIME, HORMONE REPLACEMENT THERAPY, Evaporation, MEIBOMIAN-GLAND DYSFUNCTION, LIPID LAYER THICKNESS, Tear film stability, Lipidome, eye diseases, Osmolarity, Tear film, Tears, Mucin, CONTACT-LENS WEARERS, VERSUS-HOST-DISEASE, HUMAN OCULAR SURFACE, sense organs, Dry eye disease, 3125 Otorhinolaryngology, ophthalmology, DRY-EYE DISEASE
Abstract

The members of the Tear Film Subcommittee reviewed the role of the tear film in dry eye disease (DED). The Subcommittee reviewed biophysical and biochemical aspects of tears and how these change in DED. Clinically, DED is characterized by loss of tear volume, more rapid breakup of the tear film and increased evaporation of tears from the ocular surface. The tear film is composed of many substances including lipids, proteins, mucins and electrolytes. All of these contribute to the integrity of the tear film but exactly how they interact is still an area of active research. Tear film osmolarity increases in DED. Changes to other components such as proteins and mucins can be used as biomarkers for DED. The Subcommittee recommended areas for future research to advance our understanding of the tear film and how this changes with DED. The final report was written after review by all Subcommittee members and the entire TFOS DEWS II membership. (C) 2017 Elsevier Inc. All rights reserved.

Published
2017

49. Interaction and localization of the retinitis pigmentosa protein RP2 and NSF in retinal photoreceptor cells

Author

Holopainen, Juha M., Cheng, Christina L., Molday, Laurie L., Johal, Gurp, Coleman, Jonathan, Dyka, Frank, Hii, Theresa, Ahn, Jinhi, and Molday, Robert S.

Subjects
Membrane proteins -- Structure, Membrane proteins -- Chemical properties, Photoreceptors -- Research, Retinitis pigmentosa -- Genetic aspects, Retinitis pigmentosa -- Physiological aspects, Biological sciences, Chemistry
Abstract

Biochemical studies are conducted to identify proteins in retinal cell extracts that interact with RP2 in order to define the role of RP2 in the retina and X-linked retinitis pigmentosa (XLRP). The studies have shown that RP2, in addition to serving as a regulator of ADP ribosylation factor-like 3 (Arl3), has interacted with N-ethylmaleimide sensitive factor (NSF) and the complex has played a vital role in membrane protein trafficking in photoreceptors and other cells of the retina.

Published
2010

50. Effects of DPH on DPPC-cholesterol membranes with varying concentrations of cholesterol: from local perturbations to limitations in fluorescence anisotropy experiments

Author

Franova, Miroslava, Repkov, Jarmila, Capkova, Pavla, Holopainen, Juha M., and Vattulainen, Ilpo

Subjects
Brownian motion -- Analysis, Cholesterol -- Chemical properties, Dipalmitoylphosphatidylcholine -- Chemical properties, Dipalmitoylphosphatidylcholine -- Optical properties, Molecular dynamics -- Usage, Chemicals, plastics and rubber industries
Published
2010

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