188 results on '"Holland IB"'
Search Results
2. Secretion of Escherichia coli haemolysin
- Author
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Holland Ib
- Subjects
Mammals ,Chemistry ,Molecular Sequence Data ,Hemolysin ,Protein Sorting Signals ,Hemolysin Proteins ,medicine.disease_cause ,Biochemistry ,Escherichia coli ,medicine ,Animals ,Secretion ,Amino Acid Sequence ,Peptide sequence - Published
- 1989
3. Protein secretion in Escherichia coli with particular reference to haemolysin
- Author
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Holland Ib
- Subjects
Hemolysin Proteins ,Secretory protein ,Chemistry ,medicine ,Escherichia coli ,Biological Transport, Active ,Membrane Proteins ,Hemolysin ,medicine.disease_cause ,Biochemistry ,Microbiology ,Bacterial Outer Membrane Proteins - Published
- 1989
4. Structural and functional diversity calls for a new classification of ABC transporters.
- Author
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Thomas C, Aller SG, Beis K, Carpenter EP, Chang G, Chen L, Dassa E, Dean M, Duong Van Hoa F, Ekiert D, Ford R, Gaudet R, Gong X, Holland IB, Huang Y, Kahne DK, Kato H, Koronakis V, Koth CM, Lee Y, Lewinson O, Lill R, Martinoia E, Murakami S, Pinkett HW, Poolman B, Rosenbaum D, Sarkadi B, Schmitt L, Schneider E, Shi Y, Shyng SL, Slotboom DJ, Tajkhorshid E, Tieleman DP, Ueda K, Váradi A, Wen PC, Yan N, Zhang P, Zheng H, Zimmer J, and Tampé R
- Subjects
- ATP-Binding Cassette Transporters metabolism, Protein Folding, ATP-Binding Cassette Transporters chemistry, ATP-Binding Cassette Transporters classification, Protein Domains
- Abstract
Members of the ATP-binding cassette (ABC) transporter superfamily translocate a broad spectrum of chemically diverse substrates. While their eponymous ATP-binding cassette in the nucleotide-binding domains (NBDs) is highly conserved, their transmembrane domains (TMDs) forming the translocation pathway exhibit distinct folds and topologies, suggesting that during evolution the ancient motor domains were combined with different transmembrane mechanical systems to orchestrate a variety of cellular processes. In recent years, it has become increasingly evident that the distinct TMD folds are best suited to categorize the multitude of ABC transporters. We therefore propose a new ABC transporter classification that is based on structural homology in the TMDs., (© 2020 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
- Published
- 2020
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5. Rise and rise of the ABC transporter families.
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Holland IB
- Subjects
- ATP-Binding Cassette Transporters genetics, Biological Transport physiology, Drug Resistance, Bacterial genetics, Drug Resistance, Bacterial physiology, Lipid Metabolism physiology, Protein Conformation, ATP-Binding Cassette Transporters metabolism, Adenosine Triphosphate metabolism, Anti-Bacterial Agents metabolism, Bacteria metabolism
- Abstract
This review will inevitably be influenced by my personal experience and personal view of the progression of this amazing family of proteins. This has generated a huge literature in over nearly five decades, some ideas have bloomed and faded while others have persisted, other contributions simply become redundant, overtaken by better techniques. At the outset, the pioneers had no idea of the magnitude of the topic they were working on, then a very rough idea of the significance emerged and, progressively, the picture becomes sharper and finally extraordinary. I have tried to produce at least an outline of that progression. My apologies for the also inevitable omissions, especially relating to the mass of biochemical and spectroscopy and genetical studies. I decided to prioritise structural biology because structures when successful are definitive and of course provide a 'visual' image. However, I tried to limit the structural aspects to the proteins that reflected the main advances., (Copyright © 2019. Published by Elsevier Masson SAS.)
- Published
- 2019
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6. Type I Secretion Systems-One Mechanism for All?
- Author
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Spitz O, Erenburg IN, Beer T, Kanonenberg K, Holland IB, and Schmitt L
- Subjects
- Bacterial Proteins metabolism, Bacterial Secretion Systems, Biological Transport, Protein Transport, Gram-Negative Bacteria metabolism, Type I Secretion Systems metabolism
- Abstract
Type I secretion systems (T1SS) are widespread in Gram-negative bacteria, especially in pathogenic bacteria, and they secrete adhesins, iron-scavenger proteins, lipases, proteases, or pore-forming toxins in the unfolded state in one step across two membranes without any periplasmic intermediate into the extracellular space. The substrates of T1SS are in general characterized by a C-terminal secretion sequence and nonapeptide repeats, so-called GG repeats, located N terminal to the secretion sequence. These GG repeats bind Ca
2+ ions in the extracellular space, which triggers folding of the entire protein. Here we summarize our current knowledge of how Gram-negative bacteria secrete these substrates, which can possess a molecular mass of up to 1,500 kDa. We also describe recent findings that demonstrate that the absence of periplasmic intermediates, the "classic" mode of action, does not hold true for all T1SS and that we are beginning to realize modifications of a common theme.- Published
- 2019
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7. Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145.
- Author
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Esnault C, Dulermo T, Smirnov A, Askora A, David M, Deniset-Besseau A, Holland IB, and Virolle MJ
- Subjects
- Anthraquinones metabolism, Bacterial Proteins metabolism, Glycolysis, Oxidative Stress, Polyphosphates metabolism, Secondary Metabolism, Triglycerides metabolism, Anti-Bacterial Agents metabolism, Streptomyces coelicolor metabolism, Streptomyces lividans metabolism
- Abstract
The Streptomyces genus is well known for its ability to produce bio-active secondary metabolites of great medical interest. However, the metabolic features accompanying these bio-productions remain to be defined. In this study, the comparison of related model strains producing differing levels of actinorhoddin (ACT), showed that S. lividans, a weak producer, had high TriAcylGlycerol (TAG) content indicative of a glycolytic metabolism. In contrast, the strong producer, S. coelicolor, was characterized by low TAG content, active consumption of its polyphosphate (PolyP) stores and extremely high ATP/ADP ratios. This indicated highly active oxidative metabolism that was correlated with induction of ACT biosynthesis. Interestingly, in conditions of phosphate limitation, the ppk mutant had TAG content and ACT production levels intermediary between those of S. lividans and S. coelicolor. This strain was characterized by high ADP levels indicating that Ppk was acting as an Adenosine Di Phosphate Kinase. Its absence resulted in energetic stress that is proposed to trigger an activation of oxidative metabolism to restore its energetic balance. This process, which is correlated with ACT biosynthesis, requires acetylCoA to fuel the Krebs cycle and phosphate for ATP generation by the ATP synthase coupled to the respiratory chain, resulting in low TAG and polyP content of the ACT producing strains.
- Published
- 2017
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8. Bacillus subtilis Swarmer Cells Lead the Swarm, Multiply, and Generate a Trail of Quiescent Descendants.
- Author
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Hamouche L, Laalami S, Daerr A, Song S, Holland IB, Séror SJ, Hamze K, and Putzer H
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- Culture Media chemistry, DNA Replication, Models, Theoretical, Peptidoglycan biosynthesis, Protein Biosynthesis, Bacillus subtilis growth & development
- Abstract
Bacteria adopt social behavior to expand into new territory, led by specialized swarmers, before forming a biofilm. Such mass migration of Bacillus subtilis on a synthetic medium produces hyperbranching dendrites that transiently (equivalent to 4 to 5 generations of growth) maintain a cellular monolayer over long distances, greatly facilitating single-cell gene expression analysis. Paradoxically, while cells in the dendrites (nonswarmers) might be expected to grow exponentially, the rate of swarm expansion is constant, suggesting that some cells are not multiplying. Little attention has been paid to which cells in a swarm are actually multiplying and contributing to the overall biomass. Here, we show in situ that DNA replication, protein translation and peptidoglycan synthesis are primarily restricted to the swarmer cells at dendrite tips. Thus, these specialized cells not only lead the population forward but are apparently the source of all cells in the stems of early dendrites. We developed a simple mathematical model that supports this conclusion., Importance: Swarming motility enables rapid coordinated surface translocation of a microbial community, preceding the formation of a biofilm. This movement occurs in thin films and involves specialized swarmer cells localized to a narrow zone at the extreme swarm edge. In the B. subtilis system, using a synthetic medium, the swarm front remains as a cellular monolayer for up to 1.5 cm. Swarmers display high-velocity whirls and vortexing and are often assumed to drive community expansion at the expense of cell growth. Surprisingly, little attention has been paid to which cells in a swarm are actually growing and contributing to the overall biomass. Here, we show that swarmers not only lead the population forward but continue to multiply as a source of all cells in the community. We present a model that explains how exponential growth of only a few cells is compatible with the linear expansion rate of the swarm., (Copyright © 2017 Hamouche et al.)
- Published
- 2017
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9. Type I Protein Secretion-Deceptively Simple yet with a Wide Range of Mechanistic Variability across the Family.
- Author
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Holland IB, Peherstorfer S, Kanonenberg K, Lenders M, Reimann S, and Schmitt L
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- ATP-Binding Cassette Transporters genetics, Bacterial Outer Membrane Proteins chemistry, Bacterial Outer Membrane Proteins metabolism, Bacterial Proteins chemistry, Bacterial Proteins genetics, Biological Transport, Carrier Proteins chemistry, Carrier Proteins genetics, Carrier Proteins metabolism, Escherichia coli Proteins chemistry, Escherichia coli Proteins genetics, Escherichia coli Proteins metabolism, Hemolysin Proteins chemistry, Hemolysin Proteins genetics, Hemolysin Proteins metabolism, Membrane Transport Proteins chemistry, Membrane Transport Proteins genetics, Membrane Transport Proteins metabolism, Protein Structure, Secondary, Protein Transport, ATP-Binding Cassette Transporters metabolism, Bacteria genetics, Bacteria metabolism, Bacterial Proteins metabolism, Type I Secretion Systems genetics, Type I Secretion Systems metabolism
- Abstract
A very large type I polypeptide begins to reel out from a ribosome; minutes later, the still unidentifiable polypeptide, largely lacking secondary structure, is now in some cases a thousand or more residues longer. Synthesis of the final hundred C-terminal residues commences. This includes the identity code, the secretion signal within the last 50 amino acids, designed to dock with a waiting ATP binding cassette (ABC) transporter. What happens next is the subject of this review, with the main, but not the only focus on hemolysin HlyA, an RTX protein toxin secreted by the type I system. Transport substrates range from small peptides to giant proteins produced by many pathogens. These molecules, without detectable cellular chaperones, overcome enormous barriers, crossing two membranes before final folding on the cell surface, involving a unique autocatalytic process.Unfolded HlyA is extruded posttranslationally, C-terminal first. The transenvelope "tunnel" is formed by HlyB (ABC transporter), HlyD (membrane fusion protein) straddling the inner membrane and periplasm and TolC (outer membrane). We present a new evaluation of the C-terminal secretion code, and the structure function of HlyD and HlyB at the heart of this nanomachine. Surprisingly, key details of the secretion mechanism are remarkably variable in the many type I secretion system subtypes. These include alternative folding processes, an apparently distinctive secretion code for each type I subfamily, and alternative forms of the ABC transporter; most remarkably, the ABC protein probably transports peptides or polypeptides by quite different mechanisms. Finally, we suggest a putative structure for the Hly-translocon, HlyB, the multijointed HlyD, and the TolC exit.
- Published
- 2016
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10. A Snapshot of the Extraordinary World of Social Microbiology.
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Stanley-Wall NR, Coulthurst SJ, and Holland IB
- Subjects
- Biofilms, Flagella, Humans, Mouth microbiology, Myxococcales physiology, Physarum physiology, Pseudomonas aeruginosa pathogenicity, Quorum Sensing, Microbial Consortia physiology
- Published
- 2015
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11. Phosphate Homeostasis in Conditions of Phosphate Proficiency and Limitation in the Wild Type and the phoP Mutant of Streptomyces lividans.
- Author
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Smirnov A, Esnault C, Prigent M, Holland IB, and Virolle MJ
- Subjects
- Gene Expression Regulation, Bacterial, Homeostasis, Oxidative Phosphorylation, Bacterial Proteins metabolism, Phosphates metabolism, Streptomyces lividans metabolism
- Abstract
Phosphate, as a constituent of the high energy molecules, ATP/GTP and polyphosphate, plays a crucial role in most of the metabolic processes of living organisms. Therefore, the adaptation to low Pi availability is a major challenge for bacteria. In Streptomyces, this adaptation is tightly controlled by the two component PhoR/PhoP system. In this study, the free intracellular Pi, ATP, ADP and polyP content of the wild type and the phoP mutant strain of S. lividans TK24 were analyzed at discrete time points throughout growth in Pi replete and limited media. PolyP length and content was shown to be directly related to the Pi content of the growth medium. In Pi repletion, ATP and high molecular weight (HMW) polyP contents were higher in the phoP mutant than in the WT strain. This supports the recently proposed repressive effect of PhoP on oxidative phosphorylation. High oxidative phosphorylation activity might also have a direct or indirect positive impact on HMW polyP synthesis. In Pi sufficiency as in Pi limitation, the degradation of these polymers was shown to be clearly delayed in the phoP mutant, indicating PhoP dependent expression of the enzymes involved in this degradation. The efficient storage of Pi as polyphosphate and/or its inefficient degradation in Pi in the phoP mutant resulted in low levels of free Pi and ATP that are likely to be, at least in part, responsible for the very poor growth of this mutant in Pi limitation. Furthermore, short polyP was shown to be present outside the cell, tightly bound to the mycelium via electrostatic interactions involving divalent cations. Less short polyP was found to be associated with the mycelium of the phoP mutant than with that of the WT strain, indicating that generation and externalization of these short polyP molecules was directly or indirectly dependent on PhoP.
- Published
- 2015
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12. Editorial overview: Elucidation of protein translocation pathways, part I.
- Author
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Jacob-Dubuisson F and Holland IB
- Subjects
- Humans, Protein Transport physiology, Proteins metabolism, Signal Transduction physiology
- Published
- 2015
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13. The Type 1 secretion pathway - the hemolysin system and beyond.
- Author
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Thomas S, Holland IB, and Schmitt L
- Subjects
- ATP-Binding Cassette Transporters chemistry, ATP-Binding Cassette Transporters genetics, ATP-Binding Cassette Transporters metabolism, Escherichia coli Proteins chemistry, Hemolysin Proteins chemistry, Membrane Fusion Proteins chemistry, Membrane Fusion Proteins metabolism, Protein Interaction Maps genetics, Protein Transport genetics, Urinary Tract Infections genetics, Urinary Tract Infections microbiology, Uropathogenic Escherichia coli pathogenicity, Bacterial Secretion Systems, Escherichia coli Proteins metabolism, Hemolysin Proteins metabolism, Host-Pathogen Interactions genetics
- Abstract
Type 1 secretion systems (T1SS) are wide-spread among Gram-negative bacteria. An important example is the secretion of the hemolytic toxin HlyA from uropathogenic strains. Secretion is achieved in a single step directly from the cytosol to the extracellular space. The translocation machinery is composed of three indispensable membrane proteins, two in the inner membrane, and the third in the outer membrane. The inner membrane proteins belong to the ABC transporter and membrane fusion protein families (MFPs), respectively, while the outer membrane component is a porin-like protein. Assembly of the three proteins is triggered by accumulation of the transport substrate (HlyA) in the cytoplasm, to form a continuous channel from the inner membrane, bridging the periplasm and finally to the exterior. Interestingly, the majority of substrates of T1SS contain all the information necessary for targeting the polypeptide to the translocation channel - a specific sequence at the extreme C-terminus. Here, we summarize our current knowledge of regulation, channel assembly, translocation of substrates, and in the case of the HlyA toxin, its interaction with host membranes. We try to provide a complete picture of structure function of the components of the translocation channel and their interaction with the substrate. Although we will place the emphasis on the paradigm of Type 1 secretion systems, the hemolysin A secretion machinery from E. coli, we also cover as completely as possible current knowledge of other examples of these fascinating translocation systems. This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey., (© 2013 Elsevier B.V. All rights reserved.)
- Published
- 2014
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14. The mimic chain reaction.
- Author
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Norris V, Thierry A, Amar P, Holland IB, and Molina F
- Subjects
- Bacterial Proteins chemistry, Cell Membrane chemistry, Escherichia coli chemistry, Operon, Prions chemistry, Protein Binding, Protein Conformation, Quorum Sensing, Sensitivity and Specificity, DNA, Bacterial chemistry, Peptide Library, Peptides chemistry, Proteomics methods, Receptors, Cell Surface chemistry
- Abstract
It is sometimes speculated that the equivalent of the polymerase chain reaction might be developed for identification of peptides, proteins or other molecules. In general, though, it is believed that there can be no way to amplify targets such as proteins. Natural amplification systems do, however, exist as in the case of certain autoinducer systems in bacteria. Here, we outline a possible, generic method, the mimic chain reaction, for obtaining peptides with 3-D structures that mimic the 3-D structure of their targets. These targets would include a variety of molecules, including proteins. There are therefore two categories of applications: the ability via amplification firstly to detect a known protein or other target at an extremely low concentration, and secondly to obtain a set of peptides that mimic the structure of an unknown target and that can be used to obtain a 'photofit'., (Copyright © 2012 S. Karger AG, Basel.)
- Published
- 2012
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15. ABC transporters, mechanisms and biology: an overview.
- Author
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Holland IB
- Subjects
- Adenosine Triphosphate metabolism, Hydrolysis, Models, Theoretical, ATP-Binding Cassette Transporters physiology
- Abstract
This chapter concentrates mainly on structural and mechanistic aspects of ABC (ATP-binding cassette) transporters and, as an example of the physiological significance of these proteins, on lipid transport, vitally important for human health. The chapter considers those aspects of ABC transporter function that appear reasonably well established, those that remain controversial and what appear to be emerging themes. Although we have seen dramatic progress in ABC protein studies in the last 20 years, we are still far from a detailed molecular understanding of function. Nevertheless two critical steps - capture and release of allocrites (transport substrates) involving a binding cavity in the membrane domain, and hydrolysis of ATP by the NBD (nucleotide-binding domain) dimer - are now described by persuasive and testable models: alternating access, and sequential firing of catalysis sites respectively. However, these need to be tested rigorously by more structural and biochemical studies. Other aspects considered include the level at which ATP binding and dimer activation are controlled, the nature of the power stroke delivering mechanical energy for transport, and some unexpected and intriguing differences between importers and exporters. The chapter also emphasizes that some ABC transporters, although important for elimination of toxic compounds (xenobiotics), are also increasingly seen to play crucial roles in homoeostatic regulation of membrane biogenesis and function through translocation of endogenous allocrites such as cholesterol. Another emerging theme is the identification of accessory domains and partners for ABC proteins, resulting in a corresponding widening of the range of activities. Finally, what are the prospects for translational research and ABC transporters?
- Published
- 2011
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16. Single-cell analysis in situ in a Bacillus subtilis swarming community identifies distinct spatially separated subpopulations differentially expressing hag (flagellin), including specialized swarmers.
- Author
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Hamze K, Autret S, Hinc K, Laalami S, Julkowska D, Briandet R, Renault M, Absalon C, Holland IB, Putzer H, and Séror SJ
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- Bacillus subtilis classification, Bacillus subtilis genetics, Bacillus subtilis metabolism, Bacterial Proteins genetics, Bacterial Proteins metabolism, Biota, Flagellin genetics, Gene Expression Regulation, Bacterial, Humidity, Single-Cell Analysis, Bacillus subtilis physiology, Flagellin metabolism
- Abstract
The non-domesticated Bacillus subtilis strain 3610 displays, over a wide range of humidity, hyper-branched, dendritic, swarming-like migration on a minimal agar medium. At high (70 %) humidity, the laboratory strain 168 sfp+ (producing surfactin) behaves very similarly, although this strain carries a frameshift mutation in swrA, which another group has shown under their conditions (which include low humidity) is essential for swarming. We reconcile these different results by demonstrating that, while swrA is essential for dendritic migration at low humidity (30-40 %), it is dispensable at high humidity. Dendritic migration (flagella- and surfactin-dependent) of strains 168 sfp+ swrA and 3610 involves elongation of dendrites for several hours as a monolayer of cells in a thin fluid film. This enabled us to determine in situ the spatiotemporal pattern of expression of some key players in migration as dendrites develop, using gfp transcriptional fusions for hag (encoding flagellin), comA (regulation of surfactin synthesis) as well as eps (exopolysaccharide synthesis). Quantitative (single-cell) analysis of hag expression in situ revealed three spatially separated subpopulations or cell types: (i) networks of chains arising early in the mother colony (MC), expressing eps but not hag; (ii) largely immobile cells in dendrite stems expressing intermediate levels of hag; and (iii) a subpopulation of cells with several distinctive features, including very low comA expression but hyper-expression of hag (and flagella). These specialized cells emerge from the MC to spearhead the terminal 1 mm of dendrite tips as swirling and streaming packs, a major characteristic of swarming migration. We discuss a model for this swarming process, emphasizing the importance of population density and of the complementary roles of packs of swarmers driving dendrite extension, while non-mobile cells in the stems extend dendrites by multiplication.
- Published
- 2011
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17. Crystallization and preliminary X-ray crystallographic studies of an oligomeric species of a refolded C39 peptidase-like domain of the Escherichia coli ABC transporter haemolysin B.
- Author
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Schwarz CK, Tschapek B, Jumpertz T, Jenewein S, Lecher J, Willbold D, Panjikar S, Holland IB, Smits SH, and Schmitt L
- Subjects
- Crystallization, Crystallography, X-Ray, Protein Multimerization, ATP-Binding Cassette Transporters chemistry, Bacterial Proteins chemistry, Carrier Proteins chemistry, Escherichia coli chemistry, Hemolysin Proteins chemistry, Protein Refolding
- Abstract
The ABC transporter haemolysin B (HlyB) from Escherichia coli is part of a type I secretion system that translocates a 110 kDa toxin in one step across both membranes of this Gram-negative bacterium in an ATP-dependent manner. Sequence analysis indicates that HlyB contains a C39 peptidase-like domain at its N-terminus. C39 domains are thiol-dependent peptidases that cleave their substrates after a GG motif. Interestingly, the catalytically invariant cysteine is replaced by a tyrosine in the C39-like domain of HlyB. Here, the overexpression, purification and crystallization of the isolated C39-like domain are described as a first step towards obtaining structural insights into this domain and eventually answering the question concerning the function of a degenerated C39 domain in the ABC transporter HlyB.
- Published
- 2011
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18. Bacterial metabolic 'toxins': a new mechanism for lactose and food intolerance, and irritable bowel syndrome.
- Author
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Campbell AK, Matthews SB, Vassel N, Cox CD, Naseem R, Chaichi J, Holland IB, Green J, and Wann KT
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- Bacteria drug effects, Bacterial Toxins toxicity, Calcium Signaling drug effects, Cell Proliferation drug effects, Dietary Carbohydrates metabolism, Gene Expression drug effects, Humans, Pyruvaldehyde toxicity, Bacteria metabolism, Dietary Carbohydrates toxicity, Food, Gastrointestinal Diseases microbiology, Irritable Bowel Syndrome microbiology, Lactose Intolerance microbiology
- Abstract
Lactose and food intolerance cause a wide range of gut and systemic symptoms, including gas, gut pain, diarrhoea or constipation, severe headaches, severe fatigue, loss of cognitive functions such as concentration, memory and reasoning, muscle and joint pain, heart palpitations, and a variety of allergies (Matthews and Campbell, 2000; Matthews et al., 2005; Waud et al., 2008). These can be explained by the production of toxic metabolites from gut bacteria, as a result of anaerobic digestion of carbohydrates and other foods, not absorbed in the small intestine. These metabolites include alcohols, diols such as butan 2,3 diol, ketones, acids, and aldehydes such as methylglyoxal (Campbell et al., 2005, 2009). These 'toxins' induce calcium signals in bacteria and affect their growth, thereby acting to modify the balance of microflora in the gut (Campbell et al., 2004, 2007a,b). These bacterial 'toxins' also affect signalling mechanisms in cells around the body, thereby explaining the wide range of symptoms in people with food intolerance. This new mechanism also explains the most common referral to gastroenterologists, irritable bowel syndrome (IBS), and the illness that afflicted Charles Darwin for 50 years (Campbell and Matthews, 2005a,b). We propose it will lead to a new understanding of the molecular mechanism of type 2 diabetes and some cancers., (Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2010
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19. The rate of folding dictates substrate secretion by the Escherichia coli hemolysin type 1 secretion system.
- Author
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Bakkes PJ, Jenewein S, Smits SH, Holland IB, and Schmitt L
- Subjects
- Amino Acid Substitution, Bacterial Outer Membrane Proteins genetics, Bacterial Outer Membrane Proteins metabolism, Bacterial Proteins genetics, Bacterial Proteins metabolism, Carrier Proteins genetics, Carrier Proteins metabolism, Escherichia coli genetics, Escherichia coli Proteins genetics, Hemolysin Proteins genetics, Membrane Transport Proteins genetics, Membrane Transport Proteins metabolism, Multiprotein Complexes genetics, Multiprotein Complexes metabolism, Mutation, Missense, Periplasmic Binding Proteins genetics, Periplasmic Binding Proteins metabolism, Bacterial Secretion Systems physiology, Escherichia coli metabolism, Escherichia coli Proteins metabolism, Hemolysin Proteins metabolism, Protein Folding
- Abstract
Secretion of the Escherichia coli toxin hemolysin A (HlyA) is catalyzed by the membrane protein complex HlyB-HlyD-TolC and requires a secretion sequence located within the last 60 amino acids of HlyA. The Hly translocator complex exports a variety of passenger proteins when fused N-terminal to this secretion sequence. However, not all fusions are secreted efficiently. Here, we demonstrate that the maltose binding protein (MalE) lacking its natural export signal and fused to the HlyA secretion signal is poorly secreted by the Hly system. We anticipated that folding kinetics might be limiting secretion, and we therefore introduced the "folding" mutation Y283D. Indeed this mutant fusion protein was secreted at a much higher level. This level was further enhanced by the introduction of a second MalE folding mutation (V8G or A276G). Secretion did not require the molecular chaperone SecB. Folding analysis revealed that all mutations reduced the refolding rate of the substrate, whereas the unfolding rate was unaffected. Thus, the efficiency of secretion by the Hly system is dictated by the folding rate of the substrate. Moreover, we demonstrate that fusion proteins defective in export can be engineered for secretion while still retaining function.
- Published
- 2010
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20. Mutations affecting the extreme C terminus of Escherichia coli haemolysin A reduce haemolytic activity by altering the folding of the toxin.
- Author
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Jumpertz T, Chervaux C, Racher K, Zouhair M, Blight MA, Holland IB, and Schmitt L
- Subjects
- Amino Acid Sequence, Amino Acid Substitution, Escherichia coli metabolism, Escherichia coli Proteins genetics, Hemolysin Proteins genetics, Hemolysis, Molecular Sequence Data, Mutation, Protein Folding, beta-Lactamases metabolism, Escherichia coli genetics, Escherichia coli Proteins metabolism, Hemolysin Proteins metabolism
- Abstract
Escherichia coli haemolysin A (HlyA), an RTX toxin, is secreted probably as an unfolded intermediate, by the type I (ABC transporter-dependent) pathway, utilizing a C-terminal secretion signal. However, the mechanism of translocation and post-translocation folding is not understood. We identified a mutation (hlyA99) at the extreme C terminus, which is dominant in competition experiments, blocking secretion of the wild-type toxin co-expressed in the same cell. This suggests that unlike recessive mutations which affect recognition of the translocation machinery, the hlyA99 mutation interferes with some later step in secretion. Indeed, the mutation reduced haemolytic activity of the toxin and the activity of beta-lactamase when the latter was fused to a C-terminal 23 kDa fragment of HlyA carrying the hlyA99 mutation. A second mutant (hlyAdel6), lacking the six C-terminal residues of HlyA, also showed reduced haemolytic activity and neither mutant protein regained normal haemolytic activity in in vitro unfolding/refolding experiments. Tryptophan fluorescence spectroscopy indicated differences in structure between the secreted forms of wild-type HlyA and the HlyA Del6 mutant. These results suggested that the mutations affected the correct folding of both HlyA and the beta-lactamase fusion. Thus, we propose a dual function for the HlyA C terminus involving an important role in post-translocation folding as well as targeting HlyA for secretion.
- Published
- 2010
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21. Importance of eps genes from Bacillus subtilis in biofilm formation and swarming.
- Author
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Nagorska K, Ostrowski A, Hinc K, Holland IB, and Obuchowski M
- Subjects
- Bacillus subtilis cytology, Bacillus subtilis virology, Bacterial Proteins genetics, Bacterial Proteins metabolism, Bacteriophages drug effects, Bacteriophages genetics, Biofilms drug effects, Chromosomes, Bacterial genetics, Culture Media pharmacology, Lipopeptides pharmacology, Movement drug effects, Mutagenesis, Insertional drug effects, Mutagenesis, Insertional genetics, Mutation genetics, Peptides, Cyclic pharmacology, Polysaccharides, Bacterial metabolism, Transduction, Genetic, Bacillus subtilis genetics, Bacillus subtilis physiology, Biofilms growth & development, Genes, Bacterial genetics, Polysaccharides, Bacterial genetics
- Abstract
Unicellular organisms naturally form multicellular communities, differentiate into specialized cells, and synchronize their behaviour under certain conditions. Swarming, defined as a movement of a large mass of bacteria on solid surfaces, is recognized as a preliminary step in the formation of biofilms. The main aim of this work was to study the role of a group of genes involved in exopolysaccharide biosynthesis during pellicle formation and swarming in Bacillus subtilis strain 168. To assess the role of particular proteins encoded by the group of epsI-epsO genes that form the eps operon, we constructed a series of insertional mutants. The results obtained showed that mutations in epsJ-epsN, but not in the last gene of the eps operon (epsO), have a severe effect on pellicle formation under all tested conditions. Moreover, the inactivation of 5 out of the 6 genes analysed caused total inhibition of swarming in strain 168 (that does not produce surfactin) on LB medium. Following restoration of the sfp gene (required for production of surfactin, which is essential for swarming of the wild-type bacteria), the sfp+ strains defective in eps genes (except epsO) generated significantly different patterns during swarming on synthetic B medium, as compared to the parental strain 168 sfp+.
- Published
- 2010
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22. The extraordinary diversity of bacterial protein secretion mechanisms.
- Author
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Holland IB
- Subjects
- Bacterial Proteins genetics, Membrane Proteins genetics, Protein Transport genetics, Signal Transduction genetics, Signal Transduction physiology, Bacterial Proteins metabolism, Membrane Proteins metabolism, Protein Transport physiology
- Abstract
I have tried to cover the minimal properties of the prolific number of protein secretion systems identified presently, particularly in Gram negative bacteria. New systems, however, are being reported almost by the month and certainly I have missed some. With the accumulating evidence one remains in awe of the complexity of some pathways, with the Type III, IV and VI especially fearsome and impressive. These systems illustrate that protein secretion from bacteria is not only about passage of large polypeptides across a bilayer but also through long tunnels, raising quite different questions concerning mechanisms. The mechanism of transport via the Sec-translocase-translocon is well on the way to full understanding, although a structure of a stuck intermediate would be very helpful. The understanding of the precise details of the mechanism of targeting specificity, and actual polypeptide translocation in other systems is, however, far behind. Groups willing to do the difficult (and risky) work to understand mechanism should therefore be more actively encouraged, perhaps to pursue multidisciplinary, collaborative studies. In writing this review I have become fascinated by the cellular regulatory mechanisms that must be necessary to orchestrate the complex flow of so many polypeptides, targeted by different signals to such a wide variety of transporters. I have tried to raise questions about how this might be managed but much more needs to be done in this area. Clearly, this field is very much alive and the future will be full of revealing and surprising twists in the story.
- Published
- 2010
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23. ATP regulates calcium efflux and growth in E. coli.
- Author
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Naseem R, Wann KT, Holland IB, and Campbell AK
- Subjects
- 2,4-Dinitrophenol pharmacology, Escherichia coli growth & development, Escherichia coli metabolism, Escherichia coli Proteins genetics, Gene Expression Profiling, Gene Knockout Techniques, Membrane Proteins deficiency, Membrane Proteins genetics, Mutant Proteins genetics, Oligonucleotide Array Sequence Analysis, Proton-Translocating ATPases deficiency, Transcription Factors deficiency, Transcription Factors genetics, Uncoupling Agents pharmacology, Adenosine Triphosphate metabolism, Calcium metabolism, Escherichia coli physiology, Gene Expression Regulation, Bacterial
- Abstract
Escherichia coli regulates cytosolic free Ca(2+) in the micromolar range through influx and efflux. Herein, we show for the first time that ATP is essential for Ca(2+) efflux and that ATP levels also affect generation time. A transcriptome analysis identified 110 genes whose expression responded to an increase in cytosolic Ca(2+) (41 elevated, 69 depressed). Of these, 3 transport proteins and 4 membrane proteins were identified as potential Ca(2+) transport pathways. Expression of a further 943 genes was modified after 1 h in growth medium containing Ca(2+) relative to time zero. Based on the microarray results and other predicted possible Ca(2+) transporters, the level of cytosolic free Ca(2+) was measured in selected mutants from the Keio knockout collection using intracellular aequorin. In this way, we identified a knockout of atpD, coding for a component of the F(o)F(1) ATPase, as defective in Ca(2+) efflux. Seven other putative Ca(2+) transport proteins exhibited normal Ca(2+) handling. The defect in the DeltaatpD knockout cells could be explained by a 70% reduction in ATP. One millimolar glucose or 1 mM methylglyoxal raised ATP in the DeltaatpD knockout cells to that of the wild type and restored Ca(2+) efflux. One millimolar 2,4-dinitrophenol lowered the ATP in wild type to that in the DeltaatpD cells. Under these conditions, a similar defect in Ca(2+) efflux in wild type was observed in DeltaatpD cells. Ten millimolar concentration of Ca(2+) resulted in a 30% elevation in ATP in wild type and was accompanied by a 10% reduction in generation time under these conditions. Knockouts of pitB, a potential Ca(2+) transporter, atoA, the beta subunit of acetate CoA-transferase likely to be involved in polyhydroxybutyrate synthesis, and ppk, encoding polyphosphate kinase, all indicated no defect in Ca(2+) efflux. We therefore propose that ATP is most likely to regulate Ca(2+) efflux in E. coli through an ATPase.
- Published
- 2009
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24. CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in Bacillus subtilis.
- Author
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Absalon C, Obuchowski M, Madec E, Delattre D, Holland IB, and Séror SJ
- Subjects
- Bacillus subtilis genetics, Bacterial Proteins genetics, Escherichia coli genetics, Mutagenesis, Site-Directed, Peptide Elongation Factor Tu genetics, Phosphoprotein Phosphatases genetics, Phosphorylation, Protein Serine-Threonine Kinases genetics, Serine metabolism, Substrate Specificity, Threonine metabolism, Bacillus subtilis enzymology, Bacterial Proteins metabolism, Peptide Elongation Factor Tu metabolism, Phosphoprotein Phosphatases metabolism, Protein Serine-Threonine Kinases metabolism
- Abstract
The conserved prpC, prkC, cpgA locus in Bacillus subtilis encodes respectively a Ser/Thr phosphatase, the cognate sensor kinase (containing an external PASTA domain suggested to bind peptidoglycan precursors) and CpgA, a small ribosome-associated GTPase that we have shown previously is implicated in shape determination and peptidoglycan deposition. In this study, in a search for targets of PrkC and PrpC, we showed that, in vitro, CpgA itself is phosphorylated on serine and threonine, and another GTPase, the translation factor EF-Tu, is also phosphorylated by the kinase on the conserved T384 residue. Both substrates are dephosphorylated by PrpC in vitro. In addition, we identified YezB, a 10.3 kDa polypeptide, and a component of the stressosome, as a substrate for both enzymes in vitro and apparently in vivo. We propose that the PrpC/PrkC/CpgA system constitutes an important element of a regulatory network involved in the coordination of cell wall expansion and growth in B. subtilis.
- Published
- 2009
- Full Text
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25. Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC.
- Author
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Hamze K, Julkowska D, Autret S, Hinc K, Nagorska K, Sekowska A, Holland IB, and Séror SJ
- Subjects
- Bacillus subtilis genetics, Bacterial Proteins genetics, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Mutation, Repressor Proteins genetics, Signal Transduction, Bacillus subtilis physiology, Bacterial Proteins metabolism, Gene Expression Regulation, Bacterial, Repressor Proteins metabolism
- Abstract
Highly branched dendritic swarming of B. subtilis on synthetic B-medium involves a developmental-like process that is absolutely dependent on flagella and surfactin secretion. In order to identify new swarming genes, we targeted the two-component ComPA signalling pathway and associated global regulators. In liquid cultures, the histidine kinase ComP, and the response regulator ComA, respond to secreted pheromones ComX and CSF (encoded by phrC) in order to control production of surfactin synthases and ComS (competence regulator). In this study, for what is believed to be the first time, we established that distinct early stages of dendritic swarming can be clearly defined, and that they are amenable to genetic analysis. In a mutational analysis producing several mutants with distinctive phenotypes, we were able to assign the genes sfp (activation of surfactin synthases), comA, abrB and codY (global regulators), hag (flagellin), mecA and yvzB (hag-like), and swrB (motility), to the different swarming stages. Surprisingly, mutations in genes comPX, comQ, comS, rapC and oppD, which are normally indispensable for import of CSF, had only modest effects, if any, on swarming and surfactin production. Therefore, during dendritic swarming, surfactin synthesis is apparently subject to novel regulation that is largely independent of the ComXP pathway; we discuss possible alternative mechanisms for driving srfABCD transcription. We showed that the phrC mutant, largely independent of any effect on surfactin production, was also, nevertheless, blocked early in swarming, forming stunted dendrites, with abnormal dendrite initiation morphology. In a mixed swarm co-inoculated with phrC sfp+ and phrC+ sfp (GFP), an apparently normal swarm was produced. In fact, while initiation of all dendrites was of the abnormal phrC type, these were predominantly populated by sfp cells, which migrated faster than the phrC cells. This and other results indicated a specific migration defect in the phrC mutant that could not be trans-complemented by CSF in a mixed swarm. CSF is the C-terminal pentapeptide of the surface-exposed PhrC pre-peptide and we propose that the residual PhrC 35 aa residue peptide anchored in the exterior of the cytoplasmic membrane has an apparently novel extracellular role in swarming.
- Published
- 2009
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26. In situ localisation and quantification of surfactins in a Bacillus subtilis swarming community by imaging mass spectrometry.
- Author
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Debois D, Hamze K, Guérineau V, Le Caër JP, Holland IB, Lopes P, Ouazzani J, Séror SJ, Brunelle A, and Laprévote O
- Subjects
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Bacillus subtilis metabolism, Bacterial Proteins analysis, Lipopeptides analysis, Peptides, Cyclic analysis
- Abstract
Surfactins are a family of heptacyclopeptides in which the C-terminal carbonyl is linked with the beta-hydroxy group of a fatty acid acylating the N-terminal function of a glutamic acid residue. The fatty acyl chain is 12-16 carbon atoms long. These compounds, which are secreted by the Gram-positive bacterium Bacillus subtilis in stationary phase in liquid cultures, play an important role in swarming communities on the surface of agar media in the formation of dendritic patterns. TOF secondary ion MS (TOF-SIMS) imaging was used to map surfactins within 16-17 h swarming patterns, with a 2 mum spatial resolution. Surfactins were mainly located in the central mother colony (the site of initial inoculation), in a 'ring' surrounding the pattern and along the edges of the dendrites. In the mother colony and the interior of the dendrites, surfactins with shorter chain lengths are present, whereas in the ring surrounding the swarm community and between dendrites, surfactins with longer fatty acyl chain lengths were found. A quantitative analysis by MALDI-TOF MS showed a concentration gradient of surfactin from the mother colony to the periphery. The concentration of surfactin was approximately 400 pmol/mL in the mother colony and approximately 10 pmol/mL at the base of the dendrites, decreasing to 2 pmol/mL at their tips.
- Published
- 2008
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27. pH and monovalent cations regulate cytosolic free Ca(2+) in E. coli.
- Author
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Naseem R, Holland IB, Jacq A, Wann KT, and Campbell AK
- Subjects
- Calcium pharmacology, Cation Transport Proteins genetics, Cations, Monovalent metabolism, Escherichia coli genetics, Escherichia coli Proteins genetics, Gene Deletion, Hydrogen-Ion Concentration, Ion Transport genetics, Adaptation, Physiological genetics, Calcium metabolism, Cation Transport Proteins metabolism, Escherichia coli metabolism, Escherichia coli Proteins metabolism, Potassium metabolism
- Abstract
The results here show for the first time that pH and monovalent cations can regulate cytosolic free Ca(2+) in E. coli through Ca(2+) influx and efflux, monitored using aequorin. At pH 7.5 the resting cytosolic free Ca(2+) was 0.2-0.5 microM. In the presence of external Ca(2+) (1 mM) at alkaline pH this rose to 4 microM, being reduced to 0.9 microM at acid pH. Removal of external Ca(2+) caused an immediate decrease in cytosolic free Ca(2+) at 50-100 nM s(-1). Efflux rates were the same at pH 5.5, 7.5 and 9.5. Thus, ChaA, a putative Ca(2+)/H(+)exchanger, appeared not to be a major Ca(2+)-efflux pathway. In the absence of added Na(+), but with 1 mM external Ca(2+), cytosolic free Ca(2+) rose to approximately 10 microM. The addition of Na(+)(half maximum 60 mM) largely blocked this increase and immediately stimulated Ca(2+) efflux. However, this effect was not specific, since K(+) also stimulated efflux. In contrast, an increase in osmotic pressure by addition of sucrose did not significantly stimulate Ca(2+) efflux. The results were consistent with H(+) and monovalent cations competing with Ca(2+) for a non-selective ion influx channel. Ca(2+) entry and efflux in chaA and yrbG knockouts were not significantly different from wild type, confirming that neither ChaA nor YrbG appear to play a major role in regulating cytosolic Ca(2+) in Escherichia coli. The number of Ca(2+) ions calculated to move per cell per second ranged from <1 to 100, depending on conditions. Yet a single eukaryote Ca(2+) channel, conductance 100 pS, should conduct >6 million ions per second. This raises fundamental questions about the nature and regulation of Ca(2+) transport in bacteria, and other small living systems such as mitochondria, requiring a new mathematical approach to describe such ion movements. The results have important significance in the adaptation of E. coli to different ionic environments such as the gut, fresh water and in sea water near sewage effluents.
- Published
- 2008
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28. Water-mediated protein-fluorophore interactions modulate the affinity of an ABC-ATPase/TNP-ADP complex.
- Author
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Oswald C, Jenewein S, Smits SH, Holland IB, and Schmitt L
- Subjects
- Adenosine Diphosphate chemistry, Fluorescent Dyes chemistry, Models, Molecular, Molecular Structure, Protein Binding, Protein Structure, Secondary, Adenosine Diphosphate analogs & derivatives, Adenosine Triphosphatases chemistry, Proteins chemistry, Water chemistry
- Abstract
TNP-modified nucleotides have been used extensively to study protein-nucleotide interactions. In the case of ABC-ATPases, application of these powerful tools has been greatly restricted due to the significantly higher affinity of the TNP-nucleotide for the corresponding ABC-ATPase in comparison to the non-modified nucleotides. To understand the molecular changes occurring upon binding of the TNP-nucleotide to an ABC-ATPase, we have determined the crystal structure of the TNP-ADP/HlyB-NBD complex at 1.6A resolution. Despite the higher affinity of TNP-ADP, no direct fluorophore-protein interactions were observed. Unexpectedly, only water-mediated interactions were detected between the TNP moiety and Tyr(477), that is engaged in pi-pi stacking with the adenine ring, as well as with two serine residues (Ser(504) and Ser(509)) of the Walker A motif. Interestingly, the side chains of these two serine residues adopt novel conformations that are not observed in the corresponding ADP structure. However, in the crystal structure of the S504A mutant, which binds TNP-ADP with similar affinity to the wild type enzyme, a novel TNP-water interaction compensates for the missing serine side chain. Since this water molecule is not present in the wild type enzyme, these results suggest that only water-mediated interactions provide a structural explanation for the increased affinity of TNP-nucleotides towards ABC-ATPases. However, our results also imply that in silico approaches such as docking or modeling cannot directly be applied to generate 'affinity-adopted' ADP- or ATP-analogs for ABC-ATPases.
- Published
- 2008
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29. Methylglyoxal and other carbohydrate metabolites induce lanthanum-sensitive Ca2+ transients and inhibit growth in E. coli.
- Author
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Campbell AK, Naseem R, Holland IB, Matthews SB, and Wann KT
- Subjects
- Calcium Signaling drug effects, Carbohydrate Metabolism drug effects, Carbohydrate Metabolism physiology, Cell Proliferation drug effects, Dose-Response Relationship, Drug, Drug Interactions, Escherichia coli drug effects, Acetals administration & dosage, Acetoin administration & dosage, Calcium Signaling physiology, Diacetyl administration & dosage, Escherichia coli physiology, Lanthanum administration & dosage, Pyruvaldehyde administration & dosage
- Abstract
The results here are the first demonstration of a family of carbohydrate fermentation products opening Ca2+ channels in bacteria. Methylglyoxal, acetoin (acetyl methyl carbinol), diacetyl (2,3 butane dione), and butane 2,3 diol induced Ca2+ transients in Escherichia coli, monitored by aequorin, apparently by opening Ca2+ channels. Methylglyoxal was most potent (K(1/2) = 1 mM, 50 mM for butane 2,3 diol). Ca2+ transients depended on external Ca2+ (0.1-10 mM), and were blocked by La3+ (5 mM). The metabolites affected growth, methylglyoxal being most potent, blocking growth completely up to 5 h without killing the cells. But there was no affect on the number of viable cells after 24 h. These results were consistent with carbohydrate products activating a La3+-sensitive Ca2+ channel, rises in cytosolic Ca2+ possibly protecting against certain toxins. They have important implications in bacterial-host cell signalling, and where numbers of different bacteria compete for the same substrates, e.g., the gut in lactose and food intolerance.
- Published
- 2007
- Full Text
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30. Cytosolic Ca2+ regulates protein expression in E. coli through release from inclusion bodies.
- Author
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Naseem R, Davies SR, Jones H, Wann KT, Holland IB, and Campbell AK
- Subjects
- Escherichia coli ultrastructure, Gene Expression physiology, Calcium metabolism, Cytosol metabolism, Escherichia coli metabolism, Escherichia coli Proteins biosynthesis, Escherichia coli Proteins metabolism, Inclusion Bodies metabolism, Protein Biosynthesis physiology
- Abstract
The results here are the first clear demonstration of a physiological role for cytosolic Ca(2+) in Escherichia coli by releasing a Ca(2+) binding protein, apoaequorin, from inclusion bodies. In growth medium LB the cytosolic free Ca(2+) was 0.1-0.3 microM. Addition of EGTA reduced this to <0.1 microM, whereas addition of Ca(2+) (10mM) resulted in a cytosolic free Ca(2+) of 1-2 microM for at least 2h. Ca(2+) caused a 1.5- to 2-fold increase in the level of apoaequorin induced by IPTG. Whereas EGTA induced a 50% decrease. The effect of a Ca(2+) was explained by release of protein from the inclusion bodies, together with a stabilisation of apoaequorin against degradation. Ca(2+) also reduced the generation time by 4-5 min. These results have important implications for unravelling the physiological role of cytosolic Ca(2+) in bacteria, particularly where several species are competing for the same nutrients, such as in the gut.
- Published
- 2007
- Full Text
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31. Fermentation product butane 2,3-diol induces Ca2+ transients in E. coli through activation of lanthanum-sensitive Ca2+ channels.
- Author
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Campbell AK, Naseem R, Wann K, Holland IB, and Matthews SB
- Subjects
- Calcium Signaling, Cytosol metabolism, Dose-Response Relationship, Drug, Escherichia coli, Propane pharmacology, Stereoisomerism, Butylene Glycols pharmacology, Calcium metabolism, Calcium Channels metabolism, Fermentation, Lanthanum pharmacology
- Abstract
The results here are the first demonstration of a physiological agonist opening Ca2+ channels in bacteria. Bacteria in the gut ferment glucose and other substrates, producing alcohols, diols, ketones and acids, that play a key role in lactose intolerance, through the activation of Ca2+ and other ion channels in host cells and neighbouring bacteria. Here we show butane 2,3-diol (5-200mM; half maximum 25mM) activates Ca2+ transients in E. coli, monitored by aequorin. Ca2+-transient magnitude depended on external Ca2+ (0.1-10mM). meso-Butane 2,3-diol was approximately twice as potent as 2R,3R (-) and 2S,3S (+) butane 2,3-diol. There were no detectable effects on cytosolic free Ca2+ of butane 1,3-diol, butane 1,4-diol and ethylene glycol. The glycerol fermentation product propane 1,3-diol only induced significant Ca2+ transients in 10mM external Ca2. Ca2+ butane 2,3-diol Ca2+ transients were due to activation of Ca2+ influx, followed by activation of Ca2+ efflux. The effect of butane 2,3-diol was abolished by La3+, and markedly reduced as a function of growth phase. These results were consistent with butane 2,3-diol activating a novel La3+-sensitive Ca2+ channel. They have important implications for the role of butane 2,3-diol and Ca2+ in bacterial-host cell signalling.
- Published
- 2007
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32. Verapamil, a Ca2+ channel inhibitor acts as a local anesthetic and induces the sigma E dependent extra-cytoplasmic stress response in E. coli.
- Author
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Andersen CL, Holland IB, and Jacq A
- Subjects
- Anesthetics, Local pharmacology, Bacterial Proteins biosynthesis, Bacterial Proteins genetics, Dibucaine pharmacology, Escherichia coli genetics, Escherichia coli Proteins biosynthesis, Heat-Shock Proteins biosynthesis, Membrane Potentials drug effects, Periplasmic Proteins biosynthesis, Serine Endopeptidases biosynthesis, Calcium Channel Blockers pharmacology, Escherichia coli drug effects, Sigma Factor biosynthesis, Transcription Factors biosynthesis, Verapamil pharmacology
- Abstract
Verapamil is used clinically as a Ca(2+) channel inhibitor for the treatment of various disorders such as angina, hypertension and cardiac arrhythmia. Here we study the effect of verapamil on the bacterium Escherichia coli. The drug was shown to inhibit cell division at growth sub inhibitory concentrations, independently of the SOS response. We show verapamil is a membrane active drug, with similar effects to dibucaine, a local anesthetic. Thus, both verapamil and dibucaine abolish the proton motive force and decrease the intracellular ATP concentration. This is accompanied by induction of degP expression, as a result of the activation of the RpoE (SigmaE) extra-cytoplasmic stress response, and activation of the psp operon. Such effects of verapamil, as a membrane active compound, could explain its general toxicity in eukaryotic cells.
- Published
- 2006
- Full Text
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33. A structural analysis of asymmetry required for catalytic activity of an ABC-ATPase domain dimer.
- Author
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Zaitseva J, Oswald C, Jumpertz T, Jenewein S, Wiedenmann A, Holland IB, and Schmitt L
- Subjects
- ATP-Binding Cassette Transporters genetics, Adenosine Triphosphatases genetics, Adenosine Triphosphate chemistry, Adenosine Triphosphate metabolism, Amino Acid Motifs, Amino Acid Substitution genetics, Bacterial Proteins genetics, Binding Sites, Carrier Proteins genetics, Conserved Sequence, Crystallization, Crystallography, X-Ray, Dimerization, Escherichia coli chemistry, Hemolysin Proteins, Protein Structure, Tertiary genetics, ATP-Binding Cassette Transporters chemistry, ATP-Binding Cassette Transporters metabolism, Adenosine Triphosphatases chemistry, Adenosine Triphosphatases metabolism, Bacterial Proteins chemistry, Bacterial Proteins metabolism, Carrier Proteins chemistry, Carrier Proteins metabolism, Catalytic Domain, Escherichia coli enzymology
- Abstract
The ATP-binding cassette (ABC)-transporter haemolysin (Hly)B, a central element of a Type I secretion machinery, acts in concert with two additional proteins in Escherichia coli to translocate the toxin HlyA directly from the cytoplasm to the exterior. The basic set of crystal structures necessary to describe the catalytic cycle of the isolated HlyB-NBD (nucleotide-binding domain) has now been completed. This allowed a detailed analysis with respect to hinge regions, functionally important key residues and potential energy storage devices that revealed many novel features. These include a structural asymmetry within the ATP dimer that was significantly enhanced in the presence of Mg2+, indicating a possible functional asymmetry in the form of one open and one closed phosphate exit tunnel. Guided by the structural analysis, we identified two amino acids, closing one tunnel by an apparent salt bridge. Mutation of these residues abolished ATP-dependent cooperativity of the NBDs. The implications of these new findings for the coupling of ATP binding and hydrolysis to functional activity are discussed.
- Published
- 2006
- Full Text
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34. The GTPase, CpgA(YloQ), a putative translation factor, is implicated in morphogenesis in Bacillus subtilis.
- Author
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Cladière L, Hamze K, Madec E, Levdikov VM, Wilkinson AJ, Holland IB, and Séror SJ
- Subjects
- Amino Acid Motifs, Amino Acid Sequence, Bacillus subtilis genetics, Bacterial Proteins chemistry, Bacterial Proteins genetics, Base Sequence, Cell Wall metabolism, Conserved Sequence, Dimerization, GTP Phosphohydrolases chemistry, GTP Phosphohydrolases genetics, Gene Expression Regulation, Bacterial, Guanosine Triphosphate metabolism, Isopropyl Thiogalactoside pharmacology, Molecular Sequence Data, Morphogenesis genetics, Multigene Family, Phosphoprotein Phosphatases genetics, Prokaryotic Initiation Factors chemistry, Prokaryotic Initiation Factors genetics, Bacillus subtilis growth & development, Bacterial Proteins metabolism, GTP Phosphohydrolases metabolism, Prokaryotic Initiation Factors metabolism
- Abstract
YloQ, from Bacillus subtilis, was identified previously as an essential nucleotide-binding protein of unknown function. YloQ was successfully over-expressed in Escherichia coli in soluble form. The purified protein displayed a low GTPase activity similar to that of other small bacterial GTPases such as Bex/Era. Based on the demonstrated GTPase activity and the unusual order of the yloQ G motifs, we now designate this protein as CpgA (circularly permuted GTPase). An unexpected property of this low abundance GTPase was the demonstration, using gel filtration and ultracentrifugation analysis, that the protein formed stable dimers, dependent upon the concentration of YloQ(CpgA), but independent of GTP. In order to investigate function, cpgA was placed under the control of the pspac promotor in the B. subtilis chromosome. When grown in E or Spizizen medium in the absence of IPTG, the rate of growth was significantly reduced. A large proportion of the cells exhibited a markedly perturbed morphology, with the formation of swollen, bent or 'curly' shapes. To confirm that this was specifically due to depleted CpgA a plasmid-borne cpgA under pxyl control was introduced. This restored normal cell shape and growth rate, even in the absence of IPTG, provided xylose was present. The crystal structure of CpgA(YloQ) suggests a role as a translation initiation factor and we discuss the possibility that CpgA is involved in the translation of a subset of proteins, including some required for shape maintenance.
- Published
- 2006
- Full Text
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35. The motor domains of ABC-transporters. What can structures tell us?
- Author
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Oswald C, Holland IB, and Schmitt L
- Subjects
- ATP-Binding Cassette Transporters genetics, Adenosine Triphosphate metabolism, Amino Acid Sequence, Animals, Humans, Models, Molecular, Molecular Sequence Data, Protein Binding, Protein Structure, Secondary, Protein Structure, Tertiary, Sequence Homology, Amino Acid, ATP-Binding Cassette Transporters chemistry, ATP-Binding Cassette Transporters metabolism
- Abstract
The transport of substrates across a cellular membrane is a vitally important biological function essential for cell survival. ATP-binding cassette (ABC) transporters constitute one of the largest subfamilies of membrane proteins, accomplishing this task. Mutations in genes encoding for ABC transporters cause different diseases, for example, Adrenoleukodystrophy, Stargardt disease or Cystic Fibrosis. Furthermore, some ABC transporters are responsible for multidrug resistance, presenting a major obstacle in modern cancer chemotherapy. In order to translocate the enormous variety of substrates, ranging from ions, nutrients, small peptides to large toxins, different ABC-transporters utilize the energy gained from ATP binding and hydrolysis. The ATP binding cassette, also called the motor domain of ABC transporters, is highly conserved among all ABC transporters. The ability to purify this domain rather easily presents a perfect possibility to investigate the mechanism of ATP hydrolysis, thus providing us with a detailed picture of this process. Recently, many crystal structures of the ATP-binding domain and the full-length structures of two ABC transporters have been solved. Combining these structural data, we have now the opportunity to analyze the hydrolysis event on a molecular level. This review provides an overview of the structural investigations of the ATP-binding domains, highlighting molecular changes upon ATP binding and hydrolysis.
- Published
- 2006
- Full Text
- View/download PDF
36. Molecular insights into the mechanism of ATP-hydrolysis by the NBD of the ABC-transporter HlyB.
- Author
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Hanekop N, Zaitseva J, Jenewein S, Holland IB, and Schmitt L
- Subjects
- Bacterial Proteins chemistry, Binding Sites, Carrier Proteins chemistry, Dimerization, Hemolysin Proteins, Hydrolysis, Models, Molecular, Adenosine Triphosphate metabolism, Bacterial Proteins metabolism, Carrier Proteins metabolism
- Abstract
The ABC-transporter HlyB is a central element of the Type I protein secretion machinery, dedicated to export the E. coli toxin HlyA in a single step across the two membranes of the cell envelope. Here, we discuss recent insights into the structure and the mechanism of ATP-hydrolysis by the NBD of HlyB. Combining structural and biochemical data, we have suggested that substrate-assisted catalysis (SAC), but not general base catalysis, is responsible for ATP-hydrolysis in this NBD and might also operate in other NBDs. Finally, the implications and advantages of SAC are discussed in the context of ATP-induced dimerization of the NBDs.
- Published
- 2006
- Full Text
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37. Mutations in HlyD, part of the type 1 translocator for hemolysin secretion, affect the folding of the secreted toxin.
- Author
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Pimenta AL, Racher K, Jamieson L, Blight MA, and Holland IB
- Subjects
- Amino Acid Motifs, Amino Acid Substitution, Calcium, Escherichia coli genetics, Escherichia coli physiology, Escherichia coli Proteins chemistry, Escherichia coli Proteins genetics, Escherichia coli Proteins toxicity, Hemolysin Proteins, Membrane Transport Proteins genetics, Mutation, Missense, Point Mutation, Protein Transport, DNA Mutational Analysis, Escherichia coli chemistry, Escherichia coli Proteins metabolism, Escherichia coli Proteins physiology, Membrane Transport Proteins physiology, Protein Folding
- Abstract
HlyD, a member of the membrane fusion protein family, is essential for the secretion of the RTX hemolytic toxin HlyA from Escherichia coli. Random point mutations affecting HlyA secretion were obtained, distributed in most periplasmic regions of the HlyD molecule. Analysis of the secretion phenotypes of different mutants allowed the identification of regions in HlyD involved in different steps of HlyA translocation. Four mutants, V349-I, T85-I, V334-I and L165-Q, were conditionally defective, a phenotype shown to be linked to the presence of inhibitory concentrations of Ca2+ in extracellular medium. Hly mutant T85-I was defective at an early stage in secretion, while mutants V334-I and L165-Q appeared to accumulate HlyA in the cell envelope, indicating a block at an intermediate step. Mutants V349-I, V334-I, and L165-Q were only partially defective in secretion, allowing significant levels of HlyA to be transported, but in the case of V349-I and L165-Q the HlyA molecules secreted showed greatly reduced hemolytic activity. Hemolysin molecules secreted from V349-I and V334-I are defective in normal folding and can be reactivated in vitro to the same levels as HlyA secreted from the wild-type translocator. Both V349-I and V334-I mutations mapped to the C-terminal lipoyl repeat motif, involved in the switching from the helical hairpin to the extended form of HlyD during assembly of the functional transport channel. These results suggest that HlyD is an integral component of the transport pathway, whose integrity is essential for the final folding of secreted HlyA into its active form.
- Published
- 2005
- Full Text
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38. A molecular understanding of the catalytic cycle of the nucleotide-binding domain of the ABC transporter HlyB.
- Author
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Zaitseva J, Jenewein S, Oswald C, Jumpertz T, Holland IB, and Schmitt L
- Subjects
- Adenosine Triphosphate metabolism, Amino Acid Sequence, Binding Sites, Biological Transport, Catalytic Domain, Escherichia coli metabolism, Escherichia coli Proteins chemistry, Escherichia coli Proteins metabolism, Hemolysin Proteins, Models, Molecular, Nucleotides metabolism, Protein Conformation, Protein Structure, Secondary, Bacterial Proteins chemistry, Bacterial Proteins metabolism, Carrier Proteins chemistry, Carrier Proteins metabolism
- Abstract
The ABC transporter (ATP-binding-cassette transporter) HlyB (haemolysin B) is the central element of a type I secretion machinery, dedicated to the secretion of the toxin HlyA in Escherichia coli. In addition to the ABC transporter, two other indispensable elements are necessary for the secretion of the toxin across two membranes in a single step: the transenvelope protein HlyD and the outer membrane protein TolC. Despite the fact that the hydrolysis of ATP by HlyB fuels secretion of HlyA, the essential features of the underlying transport mechanism remain an enigma. Similar to all other ABC transporters, ranging from bacteria to man, HlyB is composed of two NBDs (nucleotide-binding domains) and two transmembrane domains. Here we summarize our detailed biochemical, biophysical and structural studies aimed at an understanding of the molecular principles of how ATP-hydrolysis is coupled to energy transduction, including the conformational changes occurring during the catalytic cycle, leading to substrate transport. We have obtained individual crystal structures for each single ground state of the catalytic cycle. From these and other biochemical and mutational studies, we shall provide a detailed molecular picture of the steps governing intramolecular communication and the utilization of chemical energy, due to ATP hydrolysis, in relation to resulting structural changes within the NBD. These data will be summarized in a general model to explain how these molecular machines achieve translocation of molecules across biological membranes.
- Published
- 2005
- Full Text
- View/download PDF
39. Functional characterization and ATP-induced dimerization of the isolated ABC-domain of the haemolysin B transporter.
- Author
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Zaitseva J, Jenewein S, Wiedenmann A, Benabdelhak H, Holland IB, and Schmitt L
- Subjects
- ATP-Binding Cassette Transporters genetics, Adenosine Diphosphate metabolism, Adenosine Triphosphatases metabolism, Adenosine Triphosphate metabolism, Alanine genetics, Bacterial Proteins genetics, Buffers, Carrier Proteins genetics, Dimerization, Escherichia coli Proteins genetics, Histidine genetics, Hydrogen-Ion Concentration, Hydrolysis, Models, Chemical, Mutagenesis, Site-Directed, Peptide Fragments genetics, Peptide Fragments isolation & purification, Peptide Fragments metabolism, Protein Binding genetics, Protein Structure, Tertiary genetics, Tryptophan genetics, Tyrosine genetics, ATP-Binding Cassette Transporters isolation & purification, ATP-Binding Cassette Transporters metabolism, Adenosine Triphosphate chemistry, Bacterial Proteins chemistry, Bacterial Proteins metabolism, Carrier Proteins chemistry, Carrier Proteins metabolism, Escherichia coli Proteins chemistry, Escherichia coli Proteins metabolism, Hemolysin Proteins metabolism
- Abstract
Nucleotide-binding domains (NBD) are highly conserved constituents of ATP-binding cassette (ABC) transporters. Members of this family couple ATP hydrolysis to the transfer of various molecules across cell membranes. The NBD of the HlyB transporter, HlyB-NBD, was characterized with respect to its uncoupled ATPase activity, oligomeric state, and stability in solution. Experimental data showed that both the nature and pH of an assay buffer influenced the level of protein activity. Comparative analysis of protein stability and ATPase activity in various buffers suggests an inverse relationship between the two. The highest ATPase activity was detected in HEPES, pH 7.0. A kinetic analysis of the ATPase activity in this buffer revealed an enzyme concentration dependence and ATP-induced protein oligomerization. Assuming that the dimer is the active form of enzyme, at least half of the purified HlyB-NBD was estimated to be a dimer at 1.2 microM under the most optimal conditions for ATP hydrolysis. This is about 2 orders of magnitude lower than reported for other canonical ABC-ATPases. The maximum reaction velocity of 0.6 micromol/mg x min at 22 degrees C and the apparent kinetic constant K(app)(0.5) of 0.26 mM for ATP were determined for the dimerized HlyB-NBD. Gel filtration experiments with the wild-type protein and HlyB-NBD mutated in a key catalytic residue, H662A, provided further evidence for ATP-induced protein dimerization. ATPase activity experiments with protein mixtures composed of wild-type and the ATPase-deficient H662A mutant demonstrated that one intact NBD within a dimer is sufficient for ATP hydrolysis. This single site turnover might suggest a sequential mechanism of ATP hydrolysis in the intact HlyB transporter.
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- 2005
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40. H662 is the linchpin of ATP hydrolysis in the nucleotide-binding domain of the ABC transporter HlyB.
- Author
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Zaitseva J, Jenewein S, Jumpertz T, Holland IB, and Schmitt L
- Subjects
- ATP-Binding Cassette Transporters metabolism, Alanine chemistry, Amino Acid Substitution, Binding Sites, Catalysis, Crystallography, X-Ray, Dimerization, Escherichia coli Proteins metabolism, Glutamic Acid chemistry, Glutamine chemistry, Hemolysin Proteins metabolism, Histidine chemistry, Hydrogen Bonding, Hydrolysis, Models, Molecular, Protein Binding, Protein Conformation, Protein Structure, Tertiary, Structure-Activity Relationship, ATP-Binding Cassette Transporters chemistry, Adenosine Triphosphate metabolism, Escherichia coli enzymology, Escherichia coli Proteins chemistry, Hemolysin Proteins chemistry, Histidine physiology
- Abstract
The ABC transporter HlyB is a central element of the HlyA secretion machinery, a paradigm of Type I secretion. Here, we describe the crystal structure of the HlyB-NBD (nucleotide-binding domain) with H662 replaced by Ala in complex with ATP/Mg2+. The dimer shows a composite architecture, in which two intact ATP molecules are bound at the interface of the Walker A motif and the C-loop, provided by the two monomers. ATPase measurements confirm that H662 is essential for activity. Based on these data, we propose a model in which E631 and H662, highly conserved among ABC transporters, form a catalytic dyad. Here, H662 acts as a 'linchpin', holding together all required parts of a complicated network of interactions between ATP, water molecules, Mg2+, and amino acids both in cis and trans, necessary for intermonomer communication. Based on biochemical experiments, we discuss the hypothesis that substrate-assisted catalysis, rather than general base catalysis might operate in ABC-ATPases.
- Published
- 2005
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41. Positive co-operative activity and dimerization of the isolated ABC ATPase domain of HlyB from Escherichia coli.
- Author
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Benabdelhak H, Schmitt L, Horn C, Jumel K, Blight MA, and Holland IB
- Subjects
- ATP-Binding Cassette Transporters antagonists & inhibitors, ATP-Binding Cassette Transporters genetics, Adenosine Triphosphatases antagonists & inhibitors, Adenosine Triphosphatases genetics, Adenosine Triphosphate analogs & derivatives, Adenosine Triphosphate metabolism, Allosteric Regulation drug effects, Azides metabolism, Bacterial Proteins antagonists & inhibitors, Bacterial Proteins genetics, Carrier Proteins antagonists & inhibitors, Carrier Proteins genetics, Chromatography, Gel, Codon genetics, Dimerization, Escherichia coli genetics, Fluorescence, Hemolysin Proteins, Kinetics, Mutation genetics, Peptide Fragments chemistry, Peptide Fragments genetics, Peptide Fragments isolation & purification, Peptide Fragments metabolism, Potassium Chloride pharmacology, Protein Binding drug effects, Protein Structure, Quaternary, Protein Structure, Tertiary, Sodium Chloride pharmacology, Solubility, Temperature, Tryptophan metabolism, Ultracentrifugation, Vanadates pharmacology, ATP-Binding Cassette Transporters chemistry, ATP-Binding Cassette Transporters metabolism, Adenosine Triphosphatases chemistry, Adenosine Triphosphatases metabolism, Bacterial Proteins chemistry, Bacterial Proteins metabolism, Carrier Proteins chemistry, Carrier Proteins metabolism, Escherichia coli enzymology
- Abstract
The ATPase activity of the ABC (ATP-binding cassette) ATPase domain of the HlyB (haemolysin B) transporter is required for secretion of Escherichia coli haemolysin via the type I pathway. Although ABC transporters are generally presumed to function as dimers, the precise role of dimerization remains unclear. In the present study, we have analysed the HlyB ABC domain, purified separately from the membrane domain, with respect to its activity and capacity to form physically detectable dimers. The ATPase activity of the isolated ABC domain clearly demonstrated positive co-operativity, with a Hill coefficient of 1.7. Furthermore, the activity is (reversibly) inhibited by salt concentrations in the physiological range accompanied by proportionately decreased binding of 8-azido-ATP. Inhibition of activity with increasing salt concentration resulted in a change in flexibility as detected by intrinsic tryptophan fluorescence. Finally, ATPase activity was sensitive towards orthovanadate, with an IC50 of 16 microM, consistent with the presence of transient dimers during ATP hydrolysis. Nevertheless, over a wide range of protein or of NaCl or KCl concentrations, the ABC ATPase was only detected as a monomer, as measured by ultracentrifugation or gel filtration. In contrast, in the absence of salt, the sedimentation velocity determined by analytical ultracentrifugation suggested a rapid equilibrium between monomers and dimers. Small amounts of dimers, but apparently only when stabilized by 8-azido-ATP, were also detected by gel filtration, even in the presence of salt. These data are consistent with the fact that monomers can interact at least transiently and are the important species during ATP hydrolysis.
- Published
- 2005
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42. "Neural networks" in bacteria: making connections.
- Author
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Armitage JP, Holland IB, Jenal U, and Kenny B
- Subjects
- Bacteria genetics, Bacteria metabolism, Bacterial Proteins physiology, Chemotaxis, Gene Expression Regulation, Bacterial, Nitrogen Fixation, Phosphoenolpyruvate Sugar Phosphotransferase System physiology, Sigma Factor physiology, Bacteria cytology, Signal Transduction
- Published
- 2005
- Full Text
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43. Comparative analysis of the development of swarming communities of Bacillus subtilis 168 and a natural wild type: critical effects of surfactin and the composition of the medium.
- Author
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Julkowska D, Obuchowski M, Holland IB, and Séror SJ
- Subjects
- Culture Media, Flagella physiology, Lipopeptides, Bacillus subtilis growth & development, Peptides, Cyclic physiology
- Abstract
The natural wild-type Bacillus subtilis strain 3610 swarms rapidly on the synthetic B medium in symmetrical concentric waves of branched dendritic patterns. In a comparison of the behavior of the laboratory strain 168 (trp) on different media with that of 3610, strain 168 (trp), which does not produce surfactin, displayed less swarming activity, both qualitatively (pattern formation) and in speed of colonization. On E and B media, 168 failed to swarm; however, with the latter, swarming was arrested at an early stage of development, with filamentous cells and rafts of cells (characteristic of dendrites of 3610) associated with bud-like structures surrounding the central inoculum. In contrast, strain 168 apparently swarmed efficiently on Luria-Bertani (LB) agar, colonizing the entire plate in 24 h. However, analysis of the intermediate stages of development of swarms on LB medium demonstrated that, in comparison with strain 3610, initiation of swarming of 168 (trp) was delayed and the greatly reduced rate of expansion of the swarm was uncoordinated, with some regions advancing faster than others. Moreover, while early stages of swarming in 3610 are accompanied by the formation of large numbers of dendrites whose rapid advance involves packs of cells at the tips, strain 168 advanced more slowly as a continuous front. When sfp+ was inserted into the chromosome of 168 (trp) to reestablish surfactin production, many features observed with 3610 on LB medium were now visible with 168. However, swarming of 168 (sfp+) still showed some reduced speed and a distinctive pattern compared to swarming of 3610. The results are discussed in terms of the possible role of surfactin in the swarming process and the different modes of swarming on LB medium.
- Published
- 2005
- Full Text
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44. Adventures with ABC-proteins: highly conserved ATP-dependent transporters.
- Author
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Holland KA and Holland IB
- Subjects
- Bacteria metabolism, Bacterial Proteins chemistry, Bacterial Proteins metabolism, Carrier Proteins chemistry, Carrier Proteins metabolism, Dimerization, Hemolysin Proteins, Humans, Membrane Transport Proteins chemistry, Membrane Transport Proteins metabolism, Models, Molecular, Signal Transduction, ATP-Binding Cassette Transporters chemistry, ATP-Binding Cassette Transporters metabolism, Adenosine Triphosphate metabolism
- Abstract
The general properties of ABC transporters, from bacteria to humans, including a brief history of their initial discovery, are considered. ABC transporters, one of the largest protein super families and vital for human health, are in toto responsible for the transport of an enormous range of molecules from ions (CFTR) or anti-tumour drugs (Pgp/MDR) to large polypeptides. Nevertheless, all ABC transporters are powered by a conserved ATPase the ABC or NBD domain, using in all probability the same basic mechanism of action for the hydrolysis of ATP and its coupling to the transport process. Based on recent high resolution structures of several NBDs and an intact transporter, a model of how dimers of these important proteins function will be discussed, with particular attention to HlyB, the ABC transporter from E. coli.
- Published
- 2005
- Full Text
- View/download PDF
45. Type 1 protein secretion in bacteria, the ABC-transporter dependent pathway (review).
- Author
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Holland IB, Schmitt L, and Young J
- Subjects
- Bacterial Proteins chemistry, Bacterial Proteins physiology, Escherichia coli Proteins metabolism, Hemolysin Proteins metabolism, Protein Conformation, Protein Folding, Protein Transport, ATP-Binding Cassette Transporters chemistry, ATP-Binding Cassette Transporters physiology, Bacterial Proteins metabolism, Gram-Negative Bacteria metabolism
- Abstract
The relatively simple type 1 secretion system in gram-negative bacteria is nevertheless capable of transporting polypeptides of up to 800 kDa across the cell envelope in a few seconds. The translocator is composed of an ABC-transporter, providing energy through ATP hydrolysis (and perhaps the initial channel across the inner membrane), linked to a multimeric Membrane Fusion Protein (MFP) spanning the initial part of the periplasm and forming a continuous channel to the surface with an outer membrane trimeric protein. Proteins targeted to the translocator carry an (uncleaved), poorly conserved secretion signal of approximately 50 residues. In E. coli the HlyA toxin interacts with both the MFP (HlyD) and the ABC protein HlyB, (a half transporter) triggering, via a conformational change in HlyD, recruitment of the third component, TolC, into the transenvelope complex. In vitro, HlyA, through its secretion signal, binds to the nucleotide binding domain (NBD or ABC-ATPase) of HlyB in a reaction reversible by ATP that may mimic initial movement of HlyA into the translocation channel. HlyA is then transported rapidly, apparently in an unfolded form, to the cell surface, where folding and release takes place. Whilst recent structural studies of TolC and MFP-like proteins are providing atomic detail of much of the transport path, structural analysis of the HlyB NBD and other ABC ATPases, have revealed details of the catalytic cycle within an NBD dimer and a glimpse of how the action of HlyB is coupled to the translocation of HlyA.
- Published
- 2005
- Full Text
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46. Translocation of bacterial proteins--an overview.
- Author
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Holland IB
- Subjects
- Cell Membrane metabolism, Cell Membrane physiology, Cytoplasm metabolism, Cytoplasm physiology, Energy Metabolism physiology, Molecular Chaperones physiology, Protein Transport physiology, Bacterial Proteins metabolism
- Abstract
Recent progress in the understanding of the nature of the extraordinary variety of protein translocation systems, mainly in Gram negative bacteria, is reviewed. This takes us from the insertion of proteins into the inner membrane via the sophisticated Sec apparatus, the lethal injection of Type III proteins into host cells and on to the beautiful machine that assembles the flagellum. Attempts are made to establish some order, some common principles that might explain the variety and the complexity of some systems. The fundamentals considered are the nature of different transport signals, the nature of translocons (a wide variety of inner membrane types, outer membrane translocons are more conserved), the process of docking to translocons, the role of chaperones and the folding of transported proteins, the energetics of translocation, and prospects for future advances.
- Published
- 2004
- Full Text
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47. Branched swarming patterns on a synthetic medium formed by wild-type Bacillus subtilis strain 3610: detection of different cellular morphologies and constellations of cells as the complex architecture develops.
- Author
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Julkowska D, Obuchowski M, Holland IB, and Séror SJ
- Subjects
- Culture Media chemistry, Image Processing, Computer-Assisted, Microscopy, Video instrumentation, Temperature, Bacillus subtilis growth & development, Bacillus subtilis physiology, Movement
- Abstract
After optimizing the conditions, including nutrients and temperature, swarming of Bacillus subtilis 3610 was obtained on a synthetic, fully defined medium. The swarms formed highly branched (dendritic) patterns, generated by successive waves of moving cells. A detailed microscopic in situ analysis of swarms 1 and 2 revealed varied cell morphologies and a remarkable series of events, with cells assembling into different 'structures', as the architecture of the swarm developed. Long filamentous cells begin to form before the onset of the first swarming (11 h) and are again observed at later stages in the interior of individual mature dendrites. Swarm 2, detected at 18-22 h, is accompanied by the rapid movement of a wave of dispersed (non-filamentous) cells. Subsequently at the forward edge of this swarm, individual cells begin to cluster together, gradually forming de novo the shape of a dendrite tip with progressive lengthening of this new structure 'backwards' towards the swarm centre. In both swarms 1 and 2, after the initial clustering of cells, there is the progressive appearance of a spreading monolayer of rafts (4-5 non-filamented cells, neatly aligned). The alternative possible roles of the rafts in the development of the swarm are discussed.
- Published
- 2004
- Full Text
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48. The role of CAPS buffer in expanding the crystallization space of the nucleotide-binding domain of the ABC transporter haemolysin B from Escherichia coli.
- Author
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Zaitseva J, Holland IB, and Schmitt L
- Subjects
- ATP-Binding Cassette Transporters, Adenosine Diphosphate chemistry, Adenosine Triphosphate chemistry, Alkanesulfonic Acids chemistry, Biological Transport, Chelating Agents pharmacology, Chromatography, Gel, Crystallography, Crystallography, X-Ray, Cyclohexylamines chemistry, Dimerization, Disulfides, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Mutation, Protein Conformation, Protein Structure, Tertiary, Time Factors, X-Rays, Zinc chemistry, Adenosine Triphosphatases chemistry, Alkanesulfonic Acids pharmacology, Biochemistry methods, Buffers, Cyclohexylamines pharmacology, Escherichia coli metabolism, Hemolysin Proteins chemistry
- Abstract
Nucleotide-binding domains (NBDs), which are roughly 27 kDa in size, are conserved components of the large family of ABC (ATP-binding cassette) transporters, which includes importers and exporters. NBDs, or ABC-ATPases, supply energy for the translocation of a vast range of substrates across biological membranes. Despite their hydrophilic sequence, many NBDs readily associate in some way with membranes but demonstrate extreme instability in solution upon separation from the complete transporter. Conditions that stabilized the purified ABC domain of the Escherichia coli haemolysin A (HlyA) transporter were developed. This allowed the screening of unlimited crystallization conditions in the presence of different substrates, the performance of reproducible functional assays and the protection of 50 mg ml(-1) protein from precipitation on ice for months. As a result, it became possible to obtain crystals of HlyB-NBD in the presence of ADP and ATP that were suitable for X-ray analysis. Although the focus of these investigations was placed on HlyB-NBD, the strategy described here can be directly transferred to other proteins that display instability in solution., (Copyright 2004 International Union of Crystallography)
- Published
- 2004
- Full Text
- View/download PDF
49. In vivo expression of the mannose-resistant fimbriae of Photorhabdus temperata K122 during insect infection.
- Author
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Meslet-Cladiere LM, Pimenta A, Duchaud E, Holland IB, and Blight MA
- Subjects
- Animals, Base Sequence, Chromosome Inversion, DNA, Bacterial chemistry, Hemagglutination, Horses, Mannose, Molecular Sequence Data, Multigene Family, Operon, Reverse Transcriptase Polymerase Chain Reaction, Fimbriae, Bacterial genetics, Moths microbiology, Photorhabdus genetics, Photorhabdus pathogenicity
- Abstract
Photorhabdus temperata K122 is an entomopathogenic bacterium symbiotically associated with nematodes of the family Heterorhabditidae: Surface fimbriae are important for the colonization of many pathogenic bacteria, and here we report the nucleotide sequence and analysis of the expression of a 12-kbp fragment encoding the mannose-resistant fimbriae of P. temperata (mrf). The mrf gene cluster contains 11 genes with an organization similar to that of the mrp locus from Proteus mirabilis. mrfI (encoding a putative recombinase) and mrfA (encoding pilin), the first gene in an apparent operon of nine other genes, are expressed from divergent promoters. The mrfI-mrfA intergenic region contains inverted repeats flanking the mrfA promoter. This region was shown to be capable of inversion, consistent with an ON/OFF regulation of the operon. In in vitro liquid cultures, both orientations were detected. Nevertheless, when we analyzed the expression of all of the genes in the mrf locus by semiquantitative reverse transcription-PCR during infection of Galleria mellonella (greater wax moth) larvae, expression of mrfA was not detected until 25 h postinfection, preceding the death of the larvae at 32 h. In contrast, mrfJ (a putative inhibitor of flagellar synthesis) was expressed throughout infection. Expression of mrfI was also detected only late in infection (25 to 30 h), indicating a possible increase in inversion frequency at this stage. In both in vitro liquid cultures and in vivo larval infections, the distal genes of the operon were expressed at substantially lower levels than mrfA. These results indicate the complex regulation of the mrf cluster during infection.
- Published
- 2004
- Full Text
- View/download PDF
50. Crystal structure of the nucleotide-binding domain of the ABC-transporter haemolysin B: identification of a variable region within ABC helical domains.
- Author
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Schmitt L, Benabdelhak H, Blight MA, Holland IB, and Stubbs MT
- Subjects
- ATP-Binding Cassette Transporters genetics, ATP-Binding Cassette Transporters metabolism, Adenosine Triphosphatases genetics, Adenosine Triphosphatases metabolism, Adenosine Triphosphate metabolism, Amino Acid Sequence, Binding Sites, Crystallography, X-Ray, Escherichia coli chemistry, Escherichia coli enzymology, Escherichia coli genetics, Hemolysin Proteins genetics, Hemolysin Proteins metabolism, Methanococcus chemistry, Methanococcus enzymology, Methanococcus genetics, Models, Molecular, Molecular Sequence Data, Protein Structure, Secondary, Protein Structure, Tertiary, Sequence Homology, Amino Acid, ATP-Binding Cassette Transporters chemistry, Adenosine Triphosphatases chemistry, Hemolysin Proteins chemistry
- Abstract
The ABC-transporter haemolysin B is a central component of the secretion machinery that translocates the toxin, haemolysin A, in a Sec-independent fashion across both membranes of E. coli. Here, we report the X-ray crystal structure of the nucleotide-binding domain (NBD) of HlyB. The molecule shares the common overall architecture of ABC-transporter NBDs. However, the last three residues of the Walker A motif adopt a 3(10) helical conformation, stabilized by a bound anion. In consequence, this results in an unusual interaction between the Walker A lysine residue and the Walker B glutamate residue. As these residues are normally required to be available for ATP binding, for catalysis and for dimer formation of ABC domains, we suggest that this conformation may represent a latent monomeric form of the NBD. Surprisingly, comparison of available NBD structures revealed a structurally diverse region (SDR) of about 30 residues within the helical arm II domain, unique to each of the eight NBDs analyzed. As this region interacts with the transmembrane part of ABC-transporters, the SDR helps to explain the selectivity and/or targeting of different NBDs to their cognate transmembrane domains.
- Published
- 2003
- Full Text
- View/download PDF
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