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Background Developmental programming of chronic kidney disease (CKD) in young adults is linked to preterm birth and intrauterine growth restriction (IUGR). Which confers a higher risk of progression to chronic kidney damage in children with very low birth weight (VLBW; born weighing < 1500 g): prematurity or IUGR? Methods This is a national historical cohort study of children with VLBW cared for in perinatal medical centers in Japan. Predictive factors included three latent variables (prematurity, IUGR, stress during neonatal period) and eight observed variables (gestational age, birth weight Z-score, maternal age, duration of treatment with antibiotics and diuretics, maternal smoking, late-onset circulatory collapse, kidney dysfunction) during the perinatal period. The primary endpoint was estimated glomerular filtration rate (eGFR) at age [greater than or equal to] 3 years. A structural equation model was used to examine the pathologic constitution. Results The 446 children with VLBW included 253 boys and 193 girls, of mean age 5.8 ± 2.6 years and mean eGFR 111.7 ml/min/1.73 m.sup.2 at last encounter. Pathway analyses showed intrauterine malnutrition ([beta] = 0.85) contributed more to chronic kidney damage than stress during the neonatal period ([beta] = - 0.19) and prematurity ([beta] = 0.12), and kidney dysfunction and late-onset circulatory collapse were important observed variables in stress during the neonatal period. Conclusions IUGR was more harmful to future kidneys of VLBW neonates. Neonatal kidney dysfunction and late-onset circulatory collapse were important risk factors for subsequent CKD development. This emphasizes the need for obstetricians to monitor for fetal growth restriction and neonatologists to minimize neonatal stress to prevent CKD in later life., Author(s): Osamu Uemura [sup.1] [sup.2] [sup.3] , Kenji Ishikura [sup.4] [sup.5] , Tetsuji Kaneko [sup.6] , Daishi Hirano [sup.7] , Yuko Hamasaki [sup.8] , Masao Ogura [sup.4] , Naoaki Mikami [...]

Background Prematurity and low birth weight are risk factors for the future development of chronic kidney disease (CKD) and hypertension caused by fewer nephrons with limited filtration surface area. Few reports to date have evaluated their clinical backgrounds and pathological findings, including glomerular hypertension and focal segmental glomerulosclerosis. Case-Diagnosis/Treatment This report describes two patients, a 15-year-old girl (patient 1), with a birth weight of 618 g and a gestational age of 24 weeks, and a 14-year-old boy (patient 2), with a birth weight of 842 g and a gestational age at 25 weeks. Both had a birth weight appropriate for gestational age. Both were first diagnosed with proteinuria during adolescence, and patient 2 also had hypertension. Pathological findings included glomerulomegaly in both and hypertrophy of the juxtaglomerular apparatus and perihilar glomerulosclerosis in patient 1, suggesting glomerular hypertension. Treatment with lisinopril resulted in the immediate disappearance of proteinuria. Renal dysfunction was observed in both patients, but neither showed evidence of severe aggravation after a follow-up of 5 or 6 years. Conclusions Proteinuria in both patients was caused by glomerular hypertension with hyperfiltration. Extremely preterm birth itself may be a risk factor for future CKD. Long-term follow-up of patients born prematurely and at low birth weight, including urinalysis and blood pressure measurements, is necessary to diagnose and treat late renal complications., Author(s): Satoshi Hibino[sup.1] , Yoshifusa Abe[sup.1] , Shuichiro Watanabe[sup.2] , Yutaka Yamaguchi[sup.3] , Yuya Nakano[sup.1] , Masaru Tatsuno[sup.1] , Kazuo Itabashi[sup.1] Author Affiliations: (1) Department of Pediatrics, Showa University School [...]

Background Inherited renal tubular dysgenesis (RTD) is caused by mutations in the genes encoding the components of the renin-angiotensin system (RAS). RTD is characterized by oligohydramnios, renal failure, neonatal hypocalvaria, and severe hypotension. The histological characteristics, underlying mechanism, and long-term prognosis remain poorly known. Case-diagnosis/treatment We describe here a 4-year-old female with RTD. Endocrinologic analysis showed a discrepancy between low plasma renin activity and high active renin concentration, suggesting a loss of the renin substrate, angiotensinogen (AGT). Direct sequencing revealed a frame-shift deletion at nucleotide 1,355 in exon 5 in the AGT gene. Although a histological hallmark is regarded to be the absence or poor development of the proximal tubule, the patient does have minimally impaired function of the proximal tubule. Glomerular cysts without glomerular tufts were noted in approximately half of the glomeruli. The urinary concentrating ability and sodium reabsorption and potassium excretion in the distal nephron were severely affected. Conclusions The patient has an impaired function of the distal nephron despite minimally affected function of the proximal tubule, probably attributed to renal tubular dysgenesis and fetal hypoperfusion. The renal tubular maturity and the severity of ischemic injury may be key determinants of the clinical symptoms and pathological findings in RTD, in which the RAS plays an important role. Keywords Renal tubular dysgenesis Angiotensinogen * Rennin-angiotensin system * Nephrogenic diabetes insipidus * Glomerular cysts, Introduction Renal tubular dysgenesis (RTD) is clinically characterized by oligohydramnios, anuria, hypoplastic lung, hypocalvaria, and severe hypotension, as well as by the absence or poor development ofthe proximal tubule [1]. [...]

The enhancement of intracellular stresses such as oxidative stress and endoplasmic reticulum (ER) stress has been implicated in several neurodegenerative disorders including Parkinson's disease (PD). During a search for compounds that regulate ER stress, a dibenzoylmethane (DBM) derivative 14-26 (2,2'-dimethoxydibenzoylmethane) was identified as a novel neuroprotective agent. Analysis in SH-SY5Y cells and in PC12 cells revealed that the regulation of ER stress by 14-26 was associated with its anti-oxidative property. 14-26 prevented the production of reactive oxygen species (ROS) when the cells were exposed to oxidants such as hydrogen peroxide and 6-hydroxydopamine (6-OHDA) or an ER stressor brefeldin A (BFA). 14-26 also prevented ROS-induced damage in both the ER and the mitochondria, including the protein carbonylation in the microsome and the reduction of the mitochondrial membrane potential. Further examination disclosed the presence of the iron-chelating activity in 14-26. In vivo, 14-26 suppressed both oxidative stress and ER stress and prevented neuronal death in the substantia nigra pars compacta (SNpc) after injection of 6-OHDA in mice. These results suggest that 14-26 is an antioxidant that protects dopaminergic neurons against both oxidative stress and ER stress and could be a therapeutic candidate for the treatment of PD. neuronal cell death; stress response; Parkinson's disease

Research has been conducted on the beta-carboline alkaloids. The syntheses of these alkaloids has been performed and the results and the construction of beta-carboline-N-oxides is described.

A novel method for the synthesis of carazostatin, hyellazole and carbazoquinocins was developed based on cross-coupling reactions with 3-iodoindole and vinylstannane. The resulting intermediate produces an alcohol after reacting with ethynylmagnesium bromide followed by etherification. Successive reactions led to the formation of a triflate intermediate which forms carazostatin via palladium-catalyzed reaction.

Background: Only a few studies have investigated epidemiological and clinicopathological information regarding pediatric and adolescent and young adult (AYA) patients with renal disease. The purpose of this study was to clarify the differences and relationship of clinicopathological findings between pediatric and AYA patients using the Japan Renal Biopsy Registry (J-RBR). Methods: This cross-sectional study analyzed data from patients registered in the J-RBR between 2007 and 2017. Clinicopathological findings at diagnosis were analyzed for 3,463 pediatric (age < 15 years) and 6,532 AYA (age 15–30 years) patients. Results: Although chronic nephritic syndrome was the most common clinical diagnosis at age > 5 years, nephrotic syndrome was the most frequent diagnosis at age < 4 years. The most common pathological diagnosis as classified by pathogenesis in pediatric patients was primary glomerular disease (except IgA nephropathy), whereas IgA nephropathy was increased in AYA patients. Mesangial proliferative glomerulonephritis was the most common pathological diagnosis as classified by histopathology in both pediatric and AYA patients. Minor glomerular abnormalities were the most frequent histopathologic diagnoses of nephrotic syndrome in childhood, but their frequency decreased with age. Conclusion: To the best of our knowledge, this is the first report of clinicopathological features of pediatric and AYA patients in a large nationwide registry of renal biopsy. There were differences of clinical, pathological and histopathologic findings between pediatric and AYA patients. [ABSTRACT FROM AUTHOR]

Grossularines-2 (1b) and -1 (1a), in about 37% yields, are obtained from the reaction of N-deprotected methyl ester with p-(OMOM)phenyllithium or 3-(N-TIPS)indolyllithium, respectively. Reaction of a methyl ester with the same reagents yields 51% of 1b and 63% of 1a. A low yield of 1b is obtained from the reaction of triflate, carbon monoxide and p-(OMOM)phenylboronic acid followed by hydrolysis.

The synthesis of the 3-amino-1,4-dimethyl and 3-amino-1-methyl derivatives of 5H-pyrido(4,3-b)indole (Trp-P-1 and Trp-P-2) proceeds through the thermal electrocyclic reaction of the 1-azahexa-1,3,5-triene system involving the indole (b) bond. N-(benzenesulfonyl)indole provides the 2-vinylindole substrates via a four-step sequence. Trp-P-1 and Trp-P-2 are genotoxic heterocyclic amines produced by the overcooking of protein-containing food.