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Your search keyword '"Hazendonk, Esther"' showing total 9 results
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9 results on '"Hazendonk, Esther"'

1. Efficient targeted-selected mutagenesis in Caenorhabditis elegans: Toward a knockout for every gene

Author

Cuppen, Edwin, Gort, Eelke;, Hazendonk, Esther, Mudde, Josine, van de Belt, Jose, Nijman, Isaac J., Guryev, Victor, and Plasterk, Ronald H. A.

Subjects
Caenorhabditis elegans -- Genetic aspects, Gene mutations -- Research, Mutagenesis -- Research, Health
Abstract

A target-selected knockout approach, based on the random chemical mutagenesis is employed to study the efficient targeted-selected mutagenesis in Caenorhabditis elegans (C.elegans). The use of a nonsense introducing mutations (NIMs) genotyping approach for high-density microarrays is found to be extremely helpful for the genome-wide identification of C.elegans knockout.

Published
2007

2. Mapping determinants of gene expression plasticity by genetical genomics in C-elegans

Author

Li, Yang, Gutteling, Evert W., Tijsterman, Marcel, Fu, Jingyuan, Riksen, Joost A. G., Hazendonk, Esther, Prins, Pjotr, Plasterk, Ronald H. A., Jansen, Ritsert C., Breitling, Rainer, Kammenga, Jan E., Álvarez, Olga Alda, Li, Yang, Gutteling, Evert W., Tijsterman, Marcel, Fu, Jingyuan, Riksen, Joost A. G., Hazendonk, Esther, Prins, Pjotr, Plasterk, Ronald H. A., Jansen, Ritsert C., Breitling, Rainer, Kammenga, Jan E., and Álvarez, Olga Alda

Published
2006

4. Mapping Determinants of Gene Expression Plasticity by Genetical Genomics in C. elegans

Author

Li, Yang, primary, Álvarez, Olga Alda, additional, Gutteling, Evert W., additional, Tijsterman, Marcel, additional, Fu, Jingyuan, additional, Riksen, Joost A. G., additional, Hazendonk, Esther, additional, Prins, Pjotr, additional, Plasterk, Ronald H. A., additional, Jansen, Ritsert C., additional, Breitling, Rainer, additional, and Kammenga, Jan E., additional

Published
2006
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6. Mapping Determinants of Gene Expression Plasticity by Genetical Genomics in C. elegans

Author

Li, Yang, primary, Alvarez, Olga Alda, additional, Gutteling, Evert W, additional, Tijsterman, Marcel, additional, Fu, Jingyuan, additional, Riksen, Joost A. G., additional, Hazendonk, Esther, additional, Prins, Pjotr, additional, Plasterk, Ronald H. A., additional, Jansen, Ritsert C., additional, Breitling, Rainer, additional, and Kammenga, Jan E, additional

Published
2005
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9. Mapping Determinants of Gene Expression Plasticity by Genetical Genomics in C. elegans.

Author

Yang Li, Álvarez, Olga Alda, Gutteling, Evert W., Tijsterman, Marcel, Jingyuan Fu, Riksen, Joost A. G., Hazendonk, Esther, Prins, Pjotr, Plasterk, Ronald H. A., Jansen, Ritsert C., Breitling, Rainer, and Kammenga, Jan E.

Subjects
CAENORHABDITIS elegans, GENE mapping, GENETIC techniques, GENE expression, PHENOTYPIC plasticity, GENOMICS
Abstract

Recent genetical genomics studies have provided intimate views on gene regulatory networks. Gene expression variations between genetically different individuals have been mapped to the causal regulatory regions, termed expression quantitative trait loci. Whether the environment-induced plastic response of gene expression also shows heritable difference has not yet been studied. Here we show that differential expression induced by temperatures of 16 °C and 24 °C has a strong genetic component in Caenorhabditis elegans recombinant inbred strains derived from a cross between strains CB4856 (Hawaii) and N2 (Bristol). No less than 59% of 308 trans-acting genes showed a significant eQTL-by-environment interaction, here termed plasticity quantitative trait loci. In contrast, only 8% of an estimated 188 cis-acting genes showed such interaction. This indicates that heritable differences in plastic responses of gene expression are largely regulated in trans. This regulation is spread over many different regulators. However, for one group of trans-genes we found prominent evidence for a common master regulator: a transband of 66 coregulated genes appeared at 24 °C. Our results suggest widespread genetic variation of differential expression responses to environmental impacts and demonstrate the potential of genetical genomics for mapping the molecular determinants of phenotypic plasticity. [ABSTRACT FROM AUTHOR]

Published
2006
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