1. Characterization of a New G6PD Variant: G6PD Titusville
- Author
-
Hall Mk, Jackson S, Josef T. Prchal, Roger L. Berkow, and Magdolena Csepreghy
- Subjects
Male ,Hemolytic anemia ,Erythrocytes ,Hot Temperature ,Mutant ,Dehydrogenase ,Glucosephosphate Dehydrogenase ,Biology ,Hemolysis ,Enzyme Stability ,medicine ,Humans ,Platelet ,chemistry.chemical_classification ,Infant ,General Medicine ,medicine.disease ,Molecular biology ,In vitro ,Enzyme assay ,Red blood cell ,Glucosephosphate Dehydrogenase Deficiency ,Enzyme ,medicine.anatomical_structure ,chemistry ,Biochemistry ,Mutation ,biology.protein - Abstract
We describe a new glucose-6-phosphate dehydrogenase mutant, G-6-PD Titusville. The propositus is a 7-month-old black male infant with a transient hemolytic episode. The mutant enzyme is characterized by abnormal electrophoretic mobility, thermolability, Km for NADP, abnormal deamino NADP use and a decreased sensitivity to inhibition by NADPH. G-6-PD activity of hemolysate, as measured under optimal in vitro conditions, was not initially decreased, whereas fibroblasts, granulocytes, and platelets showed a markedly decreased level of enzyme activity. These properties identify G6PD Titusville as a unique variant of this X-linked, housekeeping enzyme. We conclude that although the propositus with G6PD Titusville had a transient hemolytic episode, we cannot be certain whether this association was a causative one.
- Published
- 1989