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Background As a candidate oncogene, PLAC8 participate in genesis and progression of various tumors. However, the role of PLAC8 in lung cancer (LC) especially non-small cell lung cancer (NSCLC) is still limited. Methods We performed Tissue microarray analysis (TMA) and Real-Time PCR (RT-PCR) to detect the expression levels of PLAC8 in LC tissues and cell lines, respectively. Then a series of cellular experiments focusing on cell proliferation, cell cycle, cell motility were conducted to identified the role of PLAC8 in NSCLC-derived cell lines H1299 and A549. Results TMA and RT-PCR showed that PLAC8 played complicated even opposite roles in different LCs. Further cellular experiments confirmed that PLAC8 could promote cell viability, alter cell cycle, and accelerate cell mobility via regulation of cell cyclins or cadherins, respectively. Conclusions Our study indicated that PLAC8 might participate in LC especially NSCLC progression. Our study also shed new light on the potential role of PLAC8 as a therapeutic biomarker in NSCLC.

An efficient and eco-friendly electrochemical synthesis of various cyclic N-sulfonylamidines from cyclic sulfamidate imines and amines via oxidative C–H/N–H cross-coupling under mild reaction conditions has been developed.

Circadian rhythm disorder (CRD) in space flight can lead to memory impairment, performance decrements and adverse health outcomes, the main manifestations of which are circadian desynchronization, sleep loss and insomnia. Sleep deprivation (SD) provide the means to evaluate these effects and the risks associated with CRD on ground. Gastrodia elata Blume (GEB) has beneficial effects on the treatment of sleep disturbances and memory loss. Fresh GEB (FG), an unprocessed raw tuber of GEB, has been used as functional health food in Asian countries for a long time. However, the research report of FG to ameliorate memory impairment caused by insomnia or lack of sleep is meager. In this study, ICR male mice were sleep-deprived continuously and water extract of FG (WFG) was orally administrated (3 and 9 g/kg/d, i.g) during the SD process lasted for 25 days, except control and model groups gavage administration with water, positive control group with modafinil (MOD, 0.1 g/kg/d, i.g). We studied the effect of WFG on CRD-induced learning and memory impairment using a set of behavioral analyses including the object location recognition test (OLRT), novel object recognition test (NORT), and the passive avoidance test (PAT). In addition, oxidative stress parameters were assessed by measuring the malondialdehyde (MDA) and superoxide dismutase (SOD) reactivity in serum and hippocampus. Our results revealed that SD decreased discrimination index (DI) in OLRT and NORT, with shorter latency into the dark chamber in PAT. Both WFG and MOD treatment can reverse these changes (P0.05). We concluded that WFG treatment improve CRD-induced learning and memory impairment and oxidative stress damage which makes FG a promising candidate as herbal health product of memory decline in CRD.

BACKGROUND Because of their cancer and treatment adverse effects, most pediatric oncology patients will experience 1 or more symptoms at one time that can seriously affect their quality of life. Because these children are attached to parents, their symptom burden directly influences the parental stress level and parental interpretations of their children's quality of life. OBJECTIVE The aim of this study was to examine the association between child-reported symptom burden and the pediatric quality of life reported by children with cancer and their parents, and whether parental perceived stress mediates these relationships. METHODS In a cross-sectional design, convenience sampling was used to recruit 80 parent-child dyads. Advanced statistical methods were adopted to analyze the mediating effects of parental stress between children's symptom burden and their quality of life. RESULTS The results revealed that parental stress was the mediator in the relationship between child-reported symptom burden and children's quality of life reported by parents. The results also showed that parental stress was not a mediator in the relationship between child-reported symptom burden and their quality of life. This underscored the differences in interpretations of quality of life reported by children and their parents. CONCLUSION Children's symptom burden is an important factor in predicting parental stress level and the quality of life reported by the children. Children's voice should be incorporated whenever possible. IMPLICATIONS FOR PRACTICE The knowledge gained from this study will facilitate intervention development to enhance parents' abilities in stress management and symptom management for their children with the support of the nursing profession.

The present study prepared a new type of Ginkgo biloba ketone ester(GBE50) preparation from polyethylene glycol and croscarmellose sodium with good biocompatibility and a certain viscosity by fused deposition modeling(FDM)-type 3D printing technique. Firstly, a cylindrical 3D printing model with a diameter of 9.00 mm and a height of 4.50 mm was established. Subsequently, the 3D-GBE50 preparations with three paths(concentric, zigzag, and grid), different layer heights, and different filling gaps were designed and prepared after the optimization of the proportions of excipients. The morphology, size, chemical properties, and dissolution activity of the 3D-GBE50 preparations were fully characterized and investigated. The results showed that 3D-GBE50 preparations had smooth appearance, clear texture, standard friability, good thermal stability, and stable chemical properties. Moreover, the printing path, layer height, and filling gap were directly related to the release rate of 3D-GBE50 preparations. The dissolution of 3D-GBE50 tablets with zigzag printing path was the fastest, while the dissolution rates of 3D-GBE50 tablets with concentric circle and grid-shaped printing paths were slower than that of commercially available G. biloba Ketone Ester Tablets. In addition, the dissolution of 3D-GBE50 tablets was faster with higher layer height and wider filling gap. As revealed by the results, th FDM-type 3D printing technique can flexibly regulate the drug release activity via controlling the printing parameters, providing effective ideas and methods for the pre-paration of personalized pharmaceutical preparations.

Background: This study evaluates the feasibility, safety, and clinical results of the self-pulling and latter transected delta-shaped anastomosis (Delta SPLT) in totally laparoscopic distal gastrec...

Background: The aim of this study was to investigate the association between children’s reported symptom burden and their parents’ quality of life, and whether parents’ perceived stress mediates this relationship. Method: this was a cross-sectional quantitative research study. Convenience sampling was used to recruit 80 pairs of parents and their children with cancer. Advanced statistical methods were used to analyse the mediating effects of parental stress between children’s symptom burden and parents’ quality of life. Results: The results showed that parental stress was the mediator in the relationship between children’s reported symptom burden and their parents’ quality of life. Conclusions: Symptom burden was prevalent in Chinese children with cancer living in the community. Children’s symptom burden is an important factor in predicting parental stress level, which simultaneously and directly lower parents’ quality of life. The evidence in this study enlarges the knowledge base about the mediating effect of parental stress on the association between the symptom burden of children with cancer and their parents’ quality of life. This evidence is crucial in paving the way for the development of interventions that improve the parental quality of life through stress-reduction programs.

Objective To improve doctors' understanding of giant perivascular space (PVS) in the brain. Methods A case of giant intracranial polycystic PVS was analyzed retrospectively by searching the databases of PubMed and Medline, combining with literature reports, the anatomy, pathophysiology, clinical manifestations, imaging changes, and treatment principles of giant PVS were summarized. Results A 19-year-old male solder was admitted due to intermittent occipital distension pain for 2 weeks. The brain MRI showed multiple cystic lesions in the right cerebral hemisphere without enhancement. The head-carotid artery CT angiography found no abnormality. The diagnosis was polycystic giant PVS in the brain. His headache was relieved after 3 days of oral compound paracetamol tablets. During a regular follow-up period for 3.5 years, he complained no discomfort. Until October 13, 2020, there were only 41 English articles about brain polycystic giant PVS collected in PubMed, including total of 46 cases. The clinical manifestations were not specific, depending on whether the nerve tissue around PVS was compressed or not. Headache accounted for 32.6%, and hydrocephalus for 43.5%. The MRI of PVS was characterized by its round, oval or tubular structure with a clear, smooth and homogeneous edge, its signal intensity was equal to that of cerebrospinal fluid (CSF) without enhancement. It is called giant or huge PVS when its diameter is more than 15 mm. There was no special treatment unless the giant PVS causes surrounding tissue oppression or hydrocephalus, if so, neurosurgical operation could be help to improve patient's status. Conclusions Characteristics of giant PVS appeared on all sequences of MIR is a CSF-like intensity cystic lesion without enhancement. Clinical attention should be paid to differential diagnosis and follow-up and. If space-occupying effect or hydrocephalus development, it can be intervened by neurosurgery, otherwise no special treatment. DOI: 10.11855/j.issn.0577-7402.2021.01.10

In this research, a novel coordination polymer (CP) based on Zn(II) ions as nodes [Zn2(1,4-NDC)2(py)2(H2O)] (1, py = pyridine and 1,4-NDCH2 = 1,4-naphthalenedicarboxylic acid) has been produced under hydrothermal reaction condition and then structurally characterized. Furthermore, Zn-complex reveals high sensitivity (with the detection limit of 1.97 × 10−6 M) and fast test time for the Al3+ in the water phase through the fluorescence enhancement method which is rare example in the reported Al3+ detection sensors. The compound was designed for hypotension treatment after anesthesia. First of all, the hypotension animal model was constructed; then, the compound’s protective was evaluated. The real-time reverse transcription-polymerase chain reaction (RT-PCR) was performed, and the α receptor expression on the vascular endothelial cells was determined. In addition to this, the western blot was also conducted for the α receptor expression assessment.

Development of highly sensitive and reliable method for detection of phytohormones is of great significance to study plant hormones and agricultural production. In this study, an ultra-high-performance liquid chromatography-mass spectrometry/mass spectrometry method was established for separation and quantification of trans-zeatin, trans-zeatin riboside, gibberellin A3, indol-3-acetic acid, salicylic acid, abscisic acid, and jasmonic acid (JA) without any label. The separation was performed on an Agilent Explus Plus C18 column by using methanol and water as mobile phases with gradient elution. The target compounds were confirmed and quantified by mass spectrum via positive electrospray ionization for trans-zeatin, trans-zeatin riboside, indole-3-acetic acid, and via negative electrospray ionization for gibberellin3, salicylic acid, abscisic acid, and JA. The limits of detection ranged from 0.0127 ng L−1 for gibberellin A3 (GA3) to 33.26 ng L−1 for JA and were lower than the currently reported values in literature. The proposed method was applied for qualitative and quantitative analyses of phytohormones in peanut gynophores and pods. The recoveries of the spiked phytohormones ranged from 80.20 to 102.56%. The contents of seven endogenous hormones varied specifically in different development stages of peanuts. This study provides a highly sensitive and selective detection method for hormones and elucidates the growth and development of the gynophore and peanut fruit, which are controlled by seven endogenous hormones.

Background: Stroke is characterized by high morbidity, high mortality, high disability rate, high recurrence rate and heavy economic burden. Post-stroke dysphagia (PSD) is one of the common complications of stroke, which can easily lead to adverse consequences such as repeated aspiration, malnutrition, pulmonary infection, and even asphyxia, and seriously affect the quality of life and prognosis of patients. For PSD patients, scientific and practical early rehabilitation measures can better improve the swallowing function, but the best time for early rehabilitation of PSD patients has not been reported. Therefore, we report on a randomized controlled trial (RCT) in which patients were offered early rehabilitation training using rehabilitation exercises for dysphagia after stroke. This protocol was written according to the SPIRIT 2013 Statement.Methods: This is a multi-center and pragmatic RCT. On the basis of sample size calculation, 180 patients with acute ischemic stroke were randomly enrolled from 3 different hospitals and divided into 4 groups: 1 control group and 3 experimental groups. The control group only received clinical routine electrical stimulation treatment, and the experimental group added rehabilitation exercise on this basis. According to the different starting time of rehabilitation exercise training after the onset of stroke, they were divided into 0-3 days group, 4-7 days group and 8-14 days group. Rehabilitation exercise training lasts for 4 weeks, once in the morning and once in the afternoon, about 30 minutes each time. Primary outcomes were the degree of improvement in patient swallowing function and efficacy assessment. Secondary outcomes, including incidence of choking, pulmonary infection, quality of life, and training compliance rates, were assessed at baseline and 4 weeks after intervention initiation.Discussion: The purpose of this study was to investigate the improvement of swallowing function in PSD patients after early rehabilitation training at different time.Trial registration: chictr.org.c, ID: ChiCTR2000029149. Full Registration on 1 May 2021. https://www.chictr.org.cn/ ChiCTR2000029149.

Compact heat exchanger is a kind of advanced heat transfer equipment with small size and high efficiency. It has wide application prospects in industry. However, due to the highly compact structure, it is difficult to measure the wall temperature of heat transfer surface by traditional test methods. For measuring the thermal performance of compact heat transfer surfaces more accurately, a single-blow transient test technique using pulse change inlet condition with optimized pulse width and matching time is developed. By turning on and off the electric air heater, the pulse change in the inlet temperature can be realized and is fitted as the superposition of a positive and a negative exponential function with a time shift. In order to reduce the effect of the uncertainty of temperature measurement, the optimum pulse width and optimum matching time is obtained by numerical calculations. By means of the newly extended test method, the heat transfer performance of a parallel-plate test core is measured and compared with the results from the literature. The analysis shows that the present pulse change technique considering the optimal pulse width and matching time have to be considered for the heat transfer surfaces with NTU > 4.5 to reduce the uncertainty in temperature measurement. The experimental results are in good agreement with those given in the literature.

Purpose: This study aimed to assess the effect of intravenous dexmedetomidine (DEX) on sevoflurane EC50 for supraglottic airway device (SAD) insertion in spontaneously breathing morbidly obese patients. Patients and methods: Thirty-eight morbidly obese patients with a body mass index 40-57 kg/m2 who were scheduled for bariatric surgery under general anesthesia requiring tracheal intubation were randomly allocated to two groups receiving the different treatments: group S, saline was given intravenously, and group D, a bolus dose of DEX 1 μg/kg was administered intravenously over 10 mins, followed by intravenous DEX infusion at a rate of 0.5 μg/kg/h. Five percent sevoflurane was initially inhaled for anesthesia induction and then end-tidal expiratory sevoflurane concentration (ETsev) was adjusted to a target value as to the modified Dixon's up-and-down method. Patients' response to SAD insertion was classified as "movement" or "no movement". The average of the midpoints of all crossover points was defined as calculated sevoflurane EC50 for successful SAD insertion. Furthermore, the probit regression analysis was used to determine sevoflurane end-tidal concentrations where 50% (EC50) and 95% (EC95) insertions of SAD were successful. After the observation was completed, flexible bronchoscope-guided intubation was performed through the SAD. Results: The calculated sevoflurane EC50 for successful SAD insertion was significantly lower in group D than in group S (1.75±0.32% vs 2.92±0.26%, p

Background: It is a debatable topic about the benefit of intravenous (IV) thrombolysis with recombinant tissue-type plasminogen activator (rt-PA) for atrial fibrillation (AF) patients with acute ischemic stroke (AIS). This study aimed to identify whether IV rt-PA could improve the short-term outcome of patients with AF-AIS.Methods: Medical data of patients with AIS onset within 72hs admitted in the department of neurologic of our hospital between January 1st, 2015 and December 31, 2020 were extracted. The AF-AIS patients were selected and divided into IV rt-PA group (group A) and non-rt-PA group (group B). The baseline characteristics, imaging changes and modified Rankin Scale (mRS) score (≤ 2 as good prognosis, > 2 as poor, = 6 as death) at discharge were obtained to compare the differences between the two groups. Logistic regression was used to analyze the factors influencing on the outcome. Results: Among a total of 1663 AIS patients, there were 280 had AF, of them 227 AF-AIS cases were conformed to the inclusion criteria, including 45 in group A and 182 in group B. All of AF-AIS patients, 48.0% had larger size of infarction and 62.1% had National Institute of Health stroke scale (NIHSS)score more than 10, the differences in the size and NIHSS between the two groups were not significant. A total of 51 cases (22.5%) died during hospitalization, the difference between group A and group B was not obvious (20.0% vs. 23.1%, P=0.658). The cumulative poor outcome (including deaths) at discharge was 75.3%, the difference between the two groups was also not significant (77.8% vs. 74.7%, P=0.671). The incidence of hemorrhagic transformation (HT) in group A was higher than that of in group B (40.0% vs. 21.4, P=0.010), the same was true for parenchymal hematoma (PH) in group A than group B (22.2% vs. 5.5%, P = 0.001). On univariate analysis, poor outcome was significantly associated with infarct size, NIHSS and PH, but not thrombolysis. The proportion of PH in patients with poor outcome between the two groups was also not remarkable. On adjusted multiple logistic regression analysis, both baseline infarction size [(P=0.013, odds ratio (OR) =4.558, 95% 95% confidence interval (CI): 1.373- 15.133] and NIHSS (PConclusion: Patients with AF-AIS had larger infarction size, higher NIHSS score, higher rate of mortality and worse outcome, for them, the IV rt-PA increased the incidence of PH except significantly improved their short-term prognosis.

Placenta-specific protein 9 (PLAC9) is a putative secretory protein that was initially identified in the placenta and is involved in cell proliferation and motility. Bioinformatics analyses revealed that PLAC9 is repressed in lung cancers (LCs), especially lung adenocarcinomas, compared to that in the paired adjacent normal tissues, indicating that PLAC9 might be involved in the pathogenesis of pulmonary diseases. To investigate the potential role of PLAC9 in the abnormal reprogramming of airway epithelial cells (AECs), a key cause of pulmonary diseases, we constructed a stable PLAC9-overexpressing human bronchial epithelial cell line (16HBE-GFP-Plac9). We utilized the proteomic approach isobaric tag for relative and absolute quantification (iTRAQ) to analyze the effect of PLAC9 on cellular protein composition. Gene ontology (GO) and pathway analyses revealed that GO terms and pathways associated with cell proliferation, cell cycle progression, and cell motility and migration were significantly enriched among the proteins regulated by PLAC9. Our in vitro results showed that PLAC9 overexpression reduced cell proliferation, altered cell cycle progression, and increased cell motility, including migration and invasion. Our findings suggest that PLAC9 inhibits cell proliferation through S phase arrest by altering the expression levels of cyclin/cyclin-dependent kinases (CDKs) and promotes cell motility, likely via the concerted actions of cyclins, E-cadherin, and vimentin. Since these mechanisms may underlie PLAC9-mediated abnormal human bronchial pathogenesis, our study provides a basis for the development of molecular targeted treatments for LCs.

To detecte the carrying rate, the type and distribution of α-Thalassemia gene mutation in Honghe Prefecture, Yunnan Province, and analyze the differences in average erythrocyte volume (MCV), mean erythrocyte hemoglobin content (MCH) and hemoglobin among different types of α-Thalassemia.The DNA samples from small cell hypochromic carriers or anemia patients and women of childbearing age who underwent hematological screening in The First People's Hospital of Honghe State was from 2015 to 2019 were enrolled and analyzed, and the mutation types and frequency of alpha-thalassemia positive rate were diagnosed by PCR reverse dot blot or PCR fluorescence dissolution curve.Among the 1 016 samples, 141(13.88%) of the patients were diagnosed as α-thalassemia. The α-thalassemia was subdivided into 3 types, silent (36.17%), minor (51.77%), and HbH disease (12.06%), and the MCV, MCH and HB levels were detected and showed a obvious decrease trend with significant difference (P0.05). The gene mutation types were 9 kinds, the deletion type gene was mainly --SEA (51.06%), followed by -αAlpha-thalassemia in Honghe prefecture of Yunnan Province shows complex genetic diversity and significant genetic heterogeneity, and the mainly type of gene mutation is --SEA and --云南省红河州141例α地中海贫血患者基因 检测结果的分析.了解云南省红河州地区人群α地中海贫血基因的携带率、基因突变的类型及分布特点，分析不同类型α地中海贫血在平均红细胞体积（MCV）和平均红细胞血红蛋白含量（MCH）及血红蛋白等中的差异性.选取并收集2015年至2019年于红河州第一人民医院行血液学筛查阳性的小细胞低色素性携带者或贫血患者及育龄妇女产检的DNA样本，采用PCR反向斑点杂交或PCR荧光溶解曲线技术诊断分析α地中海贫血阳性的突变类型及频率.在1 016例受检者中，基因诊断确诊为α地中海贫血者141例（13.88%），细分为3类，分别为静止型（36.17%）、标准型（51.77%）、HbH 病（12.06%）；在对其MCV、MCH、HB的结果进行统计分析时发现，呈下降趋势，差异显著（P0.05）；基因突变类型有9种，其中α缺失型基因以--SEA为主（51.06%），其次为-α云南省红河州地区人群α地中海贫血具有基因复杂多样性和显著的遗传异质性，以基因型--SEA和

Oscillating chemical fingerprint is a nonlinear dynamic fingerprint technology that reflects the overall redox activity of the entire system based on potential-time changes in multi-stage chemical reactions. This article summarizes the application of oscillating chemical fingerprint technology combined with mathematical analysis method in the qualitative and quantitative analysis of traditional Chinese medicine and food in recent years, including similarity analysis, principal component analysis, cluster analysis and other qua-litative analysis methods, as well as linear, logarithmic, exponential, polynomial, multivariate analysis and other quantitative analysis methods, so as to provide meaningful information for further quality control analysis of the oscillation chemical fingerprint technology in the field of traditional Chinese medicine and food.

Supramolecular hydrogels are attracting soft materials with potential applications. In this study, we synthesized a facile gelator (named 2-QF) based on phenylalanine derivative with a Quinoline group. 2-QF can assemble to form hydrogels at room temperature in different colors under low pH values. Moreover, 2-QF was triggered to form a yellow metallohydrogel (2-QF-Zn) at high pH by the coordination between 2-QF and Zn

A highly enantioselective cyclopropanation to synthesize pyrimidine-substituted diester D-A cyclopropanes is reported. Various N1-vinylpyrimidines react well with phenyliodonium ylides, delivering chiral cyclopropanes in up to 97% yield with up to 99% ee. Through simple [3+2] annulation with benzaldehyde or ethyl glyoxylate, different chiral pyrimidine nucleoside analogues with a sugar ring could be obtained.

Objective. To investigate and study the related risk factors of gastric cancer (GC) patients, to establish a high-risk scoring model of GC by multiple logistic regression analysis, and to explore the establishment of a GC screening mode with clinical opportunistic screening as the main method, and by using the pattern of opportunistic screening to establish the screening of high-risk GC patients and the choice of screening methods in the clinical outpatient work. Methods. Collected the epidemiological questionnaire of 99 GC cases and 284 non-GC patients (other chronic gastric diseases and normal) diagnosed by the General Hospital of Ningxia Medical University from October 2017 to March 2019. Serum pepsinogen (PG) levels were measured by enzyme-linked immunosorbent assay (ELISA) and confirmed Helicobacter pylori (Hp) infection in gastric mucosa tissues by Giemsa staining. Determined the high-risk factors and established a scoring model through unconditional logistic regression model analysis, and the ROC curve determined the cut-off value. Then, we followed up 26 patients of nongastric cancer patients constituted a validation group, which validated the model. Results. The high-risk factors of GC included age≥55, male, drinking cellar or well water, family history of GC, Hp infection, PGI≤43.6 μg/L, and PGI/PGII≤2.1. Established the high-risk model: Y=A×age+30×gender+30×drinking water+30×Hp infection+50×family history of GC+B×PG level. The ROC curve determined that the cut-off value for high-risk GC population was ≥155, and the area under the curve (AUC) was 0.875, the sensitivity and specificity were 87.9% and 71.5%. Conclusions. According to the risk factors of GC, using statistical methods can establish a high-risk scoring model of GC, and the score≥155 is divided into the screening cut-off value for high-risk GC population. Using this model for clinical outpatient GC screening is cost-effective and has high sensitivity and specificity.

Background: Abnormal reprogramming of airway epithelium is a key cause of pulmonary diseases. The molecular mechanism underlying the abnormal reprogramming of airway epithelial cells (AECs) remains to be elucidated. Placenta-specific protein 9 (Plac9), a putative secretory protein, initially identified in placenta, has previously been shown to affect cell proliferation and motility in human embryonic hepatic cells. Results: Interestingly, we found that Plac9 was repressed in lung cancers (LCs) compared to the corresponding normal tissues. We further investigated the role of Plac9 in human bronchial epithelial cells by constructing a stable Plac9-overexpressing cell line (16HBE-GFP-Plac9) and analyzing the effect of Plac9 on cellular protein composition by using an isobaric tag for relative and absolute quantification (iTRAQ) proteomic approach. By gene ontology (GO) and pathway analyses, we found that GO terms and pathways associated with cell proliferation, cell cycle progression, and cell motility/migration were significantly enriched among the proteins regulated by Plac9. Consistently, we observed that overexpression of Plac9 reduced cell proliferation and altered cell cycle progression. In addition, it also increased cell motility, including migration and invasion. Conclusions: Our findings suggest that Plac9 inhibits cell proliferation through S phase arrest by altering cyclins/cyclin-dependent kinases (CDKs) and promotes cell motility likely via the concerted actions of cyclins, E-cadherin and vimentin, which may underlie Plac9-mediated abnormal human bronchial pathogenesis.

A new method for qualitative and quantitative analysis of Rhodobryum giganteum by using the nonlinear oscillating chemical was established for improving the quality control standard of R. giganteum. Its potential(E)/time(t) curve was recorded by electrochemical workstation in the oscillation reaction system of BrO~-_3-Ce(SO_4)_2-H_2SO_4-malonic acid/tartaric acid. The nonlinear oscillating chemical fingerprints were investigated for repeatability, and it was found that the RSD values of the four characteristic parameters of R. giganteum were less than 4.1%, indicating a good repeatability and high precision of this experiment. After optimizing the experimental parameters such as particle size, rotation speed and temperature, a new method based on nonlinear oscillating chemical was used for qualitative and quantitative analysis of R. giganteum. The results showed that there was a good linear relationship between the induction time/the period of oscillation and the dosage of herbs(0.1-1.1 g), with the relative coefficients of 0.978 and 0.975, respectively. Besides, the highest potential showed a nonlinear relationship with the dosage of herbs, with the relative coefficient of 0.999. This method was also used to discriminate the R. giganteum and R. roseum. They were similar in appearance, but their fingerprints were quite different. Independent sample t test results showed that there were significant differences in the oscillation time, the maximum amplitude and the induction time, providing a basis for the identification of the basic sources of Herba Rhodobryi Rasei.

Symbiosis is a ubiquitous phenomenon in the biological world. A typical example of mutualism is gut microbiota and their host, especially human. Host use the gut microbiota to consume food to get the energy and nutrients necessary for survival, while the gut microbiota gains habitat and nutrition. Gut microbiota interacts with host to form a balance state that is closely related to host health, and the dysequilibrium can result in many diseases in host such as obesity, high blood pressure, inflammatory bowel disease, and cancer. The gut microbiota participates in host metabolism, organism immunity, gene expression, disease development, and drug efficacy, while it is also affected by diet, antibiotics, lifestyle, inheritance, and time. We systematically reviewed the interaction between host and gut microbiota, analyzed the application status of the gut microbiota in the treatment of host diseases, described the main problems in the treatment, and provided new ideas for the application of gut microbiota in the treatment of human diseases.

BACKGROUND Mechanical ventilation is an important part of advanced life support in the intensive care unit (ICU). This study aimed to investigate the effects of ABCDE bundle on hemodynamics in patients on mechanical ventilation (MV). MATERIAL AND METHODS This study used a cross-sectional overall controlled approach in which 143 patients on mechanical ventilation were divided into 2 groups. In the pre-ABCDE bundle group (n=70), conventional sedation and analgesia strategy were used. In the post-ABCDE bundle group (n=73), ABCDE bundle was used. Changes in hemodynamics parameters and related prognostic indicators were monitored at various time points before (T0) and at 1 d (T1), 3 d (T3), 5 d (T5), and 7 d (T7) after implementation of the 2 strategies. RESULTS Mean arterial blood pressure (MAP), central venous pressure (CVP), heart rate (HR), and oxygenation index (PaO2/FiO2) in the bundle group were improved more significantly than those in the pre-ABCDE bundle group (P

In this paper, terahertz (THz) quantum well photodetectors (QWPs) with metal-grating couplers are presented for normal-incident operation. Both one-dimensional and two-dimensional grating samples were fabricated. The photocurrent spectra and the peak responsivity were characterized. The effects of the grating period, fill factor, and shape of the grating patch were investigated. It is found that metal gratings are simple but efficient couplers for THz QWPs, and compared with the 45°-incident facet sample, the peak responsivity of the THz QWP with the optimum grating coupler is improved by 146%.