1. Reevaluation of confirmatory tests for human T-cell leukemia virus Type 1 using a luciferase immunoprecipitation system in blood donors.
- Author
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Furuta RA, Ma G, Matsuoka M, Otani S, Matsukura H, and Hirayama F
- Subjects
- Basic-Leucine Zipper Transcription Factors immunology, Blotting, Western, Donor Selection legislation & jurisprudence, Enzyme-Linked Immunosorbent Assay, False Negative Reactions, False Positive Reactions, Genes, Reporter, Genetic Vectors, HEK293 Cells, HTLV-I Antigens genetics, HTLV-I Antigens immunology, HTLV-I Infections blood, HTLV-I Infections epidemiology, HTLV-I Infections prevention & control, HTLV-I Infections virology, Human T-lymphotropic virus 1 immunology, Humans, Immunoprecipitation, Japan epidemiology, Luciferases, Renilla analysis, Luciferases, Renilla genetics, Luminescent Measurements, Predictive Value of Tests, Retroviridae Proteins immunology, Sensitivity and Specificity, Viremia virology, Blood Donors, Blood Safety methods, Donor Selection methods, HTLV-I Antibodies blood, HTLV-I Infections diagnosis, Human T-lymphotropic virus 1 isolation & purification, Viremia diagnosis
- Abstract
Background: Recently, Japanese Red Cross blood centers have changed the confirmatory test method from an indirect immunofluorescence (IF) technique to Western blotting (WB) for antibodies against human T-cell leukemia virus Type 1 (HTLV-1). In this study, these HTLV-1 tests were assessed using another sensitive method, that is, a luciferase immunoprecipitation system (LIPS), to identify a better confirmatory test for HTLV-1 infection., Study Design and Methods: Plasma samples from 54 qualified donors and 114 HTLV-1 screening-positive donors were tested by LIPS for antibodies against HTLV-1 Gag, Tax, Env, and HBZ recombinant proteins. The donors were categorized into six groups, namely, (Group I) qualified donors, screening positive; (Group II) IF positive; (Group III) IF negative; (Group IV) WB positive; (Group V) WB negative; and (Group VI) screening positive in the previous blood donation, but WB-indeterminate during this study period., Results: In Groups II and IV, all plasma samples tested positive by LIPS for antibodies against Gag and Env proteins. In Group V, all samples tested negative by LIPS, whereas some Group III samples reacted with single or double antigens in LIPS. In Group VI, the LIPS test identified a donor with suspected HTLV-1 infection. The first case of a blood donor with plasma that reacted with HBZ was identified by LIPS., Conclusion: Reevaluation of the current HTLV-1 screening method using the LIPS test showed that both confirmatory tests had similar sensitivity and specificity only when WB indeterminate results were eliminated. LIPS is a promising method for detecting and characterizing HTLV-1 antibodies., (© 2014 AABB.)
- Published
- 2015
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