Back to Search Start Over

Type-specific antigens for serological discrimination of HTLV-I and HTLV-II infection.

Authors :
Chen YM
Lee TH
Wiktor SZ
Shaw GM
Murphy EL
Blattner WA
Essex M
Source :
Lancet (London, England) [Lancet] 1990 Nov 10; Vol. 336 (8724), pp. 1153-5.
Publication Year :
1990

Abstract

55 HTLV-I (human T-cell lymphotropic virus) and 45 HTLV-II carriers, confirmed by HTLV-type specific polymerase chain reaction (PCR), were distinguished by western blot assays with recombinant HTLV I or II envelope glycoproteins. Recombinant protein (RP) B1 contains aminoacids 166-201 from HTLV-I exterior glycoprotein gp46 and was reactive with HTLV-I samples only. RP-IIB, which contains aminoacids 96-235 from HTLV-II exterior glycoprotein gp52, was reactive with all HTLV-II samples. 39 patients (86.6%) had high reactivity by densitometry. Of 55 HTLV-I samples, 35 (65.5%) had antibody reactivity to RP-IIB, but only 1 (1.8%) had high reactivity by densitometry. RP B1 and IIB western blot assays may replace the PCR test in diagnosis of HTLV infection.

Details

Language :
English
ISSN :
0140-6736
Volume :
336
Issue :
8724
Database :
MEDLINE
Journal :
Lancet (London, England)
Publication Type :
Academic Journal
Accession number :
1978028
Full Text :
https://doi.org/10.1016/0140-6736(90)92769-e