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3-dimensional, accurate, and up-to-date maps are essential for vehicles with autonomous capabilities, whose functionality is made possible by machine learning-based algorithms. Since these solutions require a tremendous amount of data for parameter optimization, simulation-to-reality (Sim2Real) methods have been proven immensely useful for training data generation. For creating realistic models to be used for synthetic data generation, crowdsourcing techniques present a resource-efficient alternative. In this paper, we show that using the Carla simulation environment, a crowdsourcing model can be created that mimics a multi-agent data gathering and processing pipeline. We developed a solution that yields dense point clouds based on monocular images and location information gathered by individual data acquisition vehicles. Our method provides scene reconstructions using the robust Structure-from-Motion (SfM) solution of Colmap. Moreover, we introduce a solution for synthesizing dense ground truth point clouds originating from the Carla simulator using a simulated data acquisition pipeline. We compare the results of the Colmap reconstruction with the reference point cloud after aligning them using the iterative closest point algorithm. Our results show that a precise point cloud reconstruction was feasible with this crowdsourcing-based approach, with 54\% of the reconstructed points having an error under 0.05 m, and a weighted root mean square error of 0.0449 m for the entire point cloud.

The distribution of many species is fragmented due to human activities and isolated populations are often affected by genetic changes. Small populations are endangered by inbreeding depression, often leading to a low mean fitness of small populations and to an increased extinction risk. Using museum samples of protected Microtus oeconomus mehelyi from the years 1950, 1964 and 2000, we studied the population which inhabits the Kis Balaton area that has considerably changed by human activity in the last centuries. The detected effective population size, differences in allele frequencies, associations between alleles and a high genetic differentiation may be explained by 50 years of random genetic drift in this population in the significantly altered Kis Balaton area. We have detected a genetic difference between Kis Balaton populations and published populations from Hungary, Austria and Slovakia. Using linkage disequilibrium and temporal approaches, the estimates of the effective population size are below 100/1000, thresholds to limit inbreeding depression and to retain evolutionary potential. Taking the fragmented occurrence of Microtus oeconomus mehelyi in central Europe into account, it is possible that also other populations may also undergo the same processes after significant isolation.

In the introductory programming education, during coding input and output management often suppresses in proportion the essential parts of the code. A novice programmer is essentially given three tasks with the solution of the original problem: reading the input, processing the data and writing the output. This article attempts to explore the role of input and output in the code, and in some cases, how to make them easier to implement.

The natural hosts of Orthohantaviruses are rodents, soricomorphs and bats, and it is well known that they may cause serious or even fatal diseases among humans worldwide. The virus is persistent among animals and it is shed via urine, saliva and feces throughout the entirety of their lives. We aim to identify the effectiveness of hantavirus detection in rodent tissue samples and urine originating from naturally infected rodents. Initially, animals were trapped at five distinct locations throughout the Transdanubian region in Hungary. Lung, liver, kidney and urine samples were obtained from 163 deceased animals. All organs and urine were tested using nested reverse transcription polymerase chain reaction (nRT-PCR). Furthermore, sera were examined for IgG antibodies against Dobrava–Belgrade virus (DOBV) and Puumala virus (PUUV) by Western blot assay. IgG antibodies against hantaviruses and/or nucleic acid were detected in 25 (15.3%) cases. Among Apodemus, Myodes, and Microtus rodent species, DOBV, PUUV and Tula virus (TULV) were clearly identified. Amid the PCR-positive samples, the nucleic acid of the viruses was detected most effectively in the kidney (100%), while only 55% of screened lung tissues were positive. Interestingly, only three out of 20 rodent urine samples were positive when tested using nRT-PCR. Moreover, five rodents were seropositive without detectable virus nucleic acid in any of the tested organs.

Common voles (Microtus arvalis) are widespread in the European agricultural landscape from central Spain to central Russia. During population outbreaks, significant damage to a variety of crops is caused and the risk of pathogen transmission from voles to people increases. In 2019, increasing or unusually high common vole densities have been reported from several European countries. This is highly important in terms of food production and public health. Therefore, authorities, extension services and farmers need to be aware of the rapid and widespread increase in common voles and take appropriate measures as soon as possible. Management options include chemical and non-chemical methods. However, the latter are suitable only for small and valuable crops and it is recommended to increase efforts to predict common voles outbreaks and to develop and field test new and optimized management tools.

Hantaviruses are worldwide pathogens, which often cause serious or even fatal diseases in humans. Hosts are predominantly in the form of rodents and soricomorphs; however, bats are also described as an important reservoir. In Hungary, representatives of two human pathogenic species of the genus Orthohantavirus are present: the Dobrava-Belgrade orthohantavirus and Puumala orthohantavirus. In Hungarian forests, the dominant rodent species are Apodemus flavicollis, Apodemus agrarius, Apodemus sylvaticus, and Myodes glareolus, all of which are natural reservoirs comprising different hantaviruses. The aim of the study was to survey the prevalence of hantaviruses among rodent populations and examine the potential relationship regarding population densities, years, sex, and seroprevalence. Rodents were trapped at 13 sampling plots in a forest reserve located in the Mecsek Mountain range, Hungary, from March to October between 2011 and 2014. Rodent serum samples were tested for IgG antibodies against Dobrava-Belgrade virus and Puumala virus by enzyme-linked immunosorbent assay (ELISA) using a recombinant nucleocapsid protein. During the 4-year sampling period, 2491 specimens were tested and 254 (10.2%) proved seropositive for orthohantaviruses. In 2011, the seroprevalence among Apodemus spp. and M. glareolus was 17.2% (114/661) and 3.9% (3/77), respectively, although this rate had reversed itself in 2014. Seropositivity was substantiated in 18.4% (12/65) of Myodes voles, while only 3.6% (13/359) of the tested Apodemus rodents were found to be IgG positive. Seroconversion was observed in 58 cases, while seroreversion was only detected in 3 individual cases. A significant difference among the number of infected males and females was identified in the first 2 years of our study. Winter survival with respect to rodents was not negatively affected due to the hantavirus infection. Hantavirus seroprevalence was not directly influenced by host abundance. Consequently, we assume that high rodent density alone does not lead to an increased risk of hantavirus infection among the rodent host population.

Background. Species identification from recovered fish scales, e.g., from the sediment or stomach contents, has proven to be problematic for clupeid species. To ensure reliable species discrimination within Clupeidae, we attempted to reveal inter- and intra-specific, as well as, the intra-individual variability of scale shape in two sympatric clupeid species the European pilchard, Sardina pilchardus (Walbaum, 1792) and the round sardinella, Sardinella aurita Valenciennes, 1847. Our aim was to test, whether the landmark-based geometric morphometric method is reliable for species identification from mixed samples. Materials and methods. The specimens of European pilchard and round sardinella were collected between May and October 2014 from the Gulf of Ambracia, Greece. The scale samples were taken from ten body areas (marked by letters A–J) of 487 specimens in total. A multiple-step analysis was conducted on randomly selected blind samples. The differences between body areas were assessed with canonical variate analysis (CVA) and discriminant function analysis (DFA) to compute generalized Mahalanobis distances (D) and discriminant functions (T2), respectively. Results. The CVA based on the total scale samples showed that in sardinella, all body areas differed significantly from each other (D = 2.54 ± 0.79, P < 0.001) except for one (area I vs. J: D = 22.36, P = 0.085). In pilchard, two body area pairs represented non-significant group pairs (area D vs. G: D = 1.03, P = 0.12; and area F vs. G: D = 1.21, P = 0.06), whereas all other areas were significantly different (D = 2.43 ± 0.88, P = 0.003). At the last step of the blind sampling procedure, all scales were correctly classified by species with an average discrimination rate as high as 96.3%. Conclusion. In contrast to scale identification based merely on morphology, the geometric morphometric method is capable of detecting differences in scale shape from mixed samples that are unattainable with the former approach. Therefore, a more reliable and effective species identification can be accomplished from recovered scales, especially when discriminating species within the same family or genus.

A novel mammalian orthoreovirus (MRV) strain was isolated from the lung tissue of a common vole (Microtus arvalis) with Tula hantavirus infection. Seven segments (L1-L3, M2-M3, S2, and S4) of the Hungarian MRV isolate MORV/47Ma/06 revealed a high similarity with an MRV strain detected in bank vole (Myodes glareolus) in Germany. The M1 and S3 segment of the Hungarian isolate showed the closest relationship with the sequence of a Slovenian human and a French murine isolate, respectively. The highest nucleotide and amino acid identity values were above 90 and 95% in all of the comparisons to the reference sequences in GenBank, except for the S1 with a maximum of 69.6% nucleotide and 75.4% amino acid identity. As wild rodents are among the main sources of zoonotic infections, the reservoir role of these animals and zoonotic potential of rodent origin MRVs need to be further investigated.

Hepatitis E virus is a major causative agent of acute hepatitis worldwide. Despite its zoonotic potential, there is limited information about the natural chain of hepevirus infection in wildlife, and the potential reservoir species. In this study, we performed a HEV survey by heminested RT-PCR on rodent samples from an urban environment (in the city of Pecs, Hungary) and investigated the prevalence of the virus among these native rodent species (Apodemus agrarius, Apodemus flavicollis, Apodemus sylvaticus, Microtus arvalis and Myodes glareolus). HEV was detected exclusively in Common voles (M. arvalis), in 10.2% of screened voles, and 3.2% of all investigated samples from all species. Based on the phylogenetic analysis, our strain showed the closest homology with European Orthohepevirus C strains detected previously in faecal samples of birds of prey and Red fox, supporting the possibility of the dietary origin of these strains. In addition, our samples showed close phylogenetic relation with a South American strain detected in Necromys lasiurus (Cricetidae), but separated clearly from other Muridae-associated strains, suggesting the presence of a Cricetidae-specific genotype in Europe and South-America. Based on these results, we hypothesize the reservoir role of M. arvalis rodents for the European Cricetidae-specific Orthohepevirus C genotype.

Rodents are important reservoir hosts for several zoonotic pathogens that cause significant morbidity and mortality in humans. Among others, leptospirosis is one of the most widespread zoonotic diseases worldwide and has the similar clinical manifestation with hantavirus infection in humans. Despite the fact that both pathogens have great epidemiological significance in Europe, no epizootiological data exist for urbanized areas so far. Therefore, this study was conducted to investigate the occurrence and prevalence of Leptospira spp. and hantaviruses in small wild rodents living in close proximity to humans. Altogether, 338 small rodents representing five different species (Apodemus agrarius, A. flavicollis, A. sylvaticus, Microtus arvalis, and Myodes glareolus) were captured in the city of Pécs (Hungary) and screened for pathogens by different types of PCR methods (TaqMan-based real-time PCR/PCR, RT-PCR/PCR). A total of 18.3% of the rodents were positive for Leptospira kirschneri, L. interrogans, and L. borgpetersenii. Nucleic acid of Tula hantavirus and human pathogen Dobrava-Belgrade orthohantavirus were detected in 8% of tested specimens. Furthermore, dual infections with both Leptospira spp. and hantaviruses were shown in 2.6% of animals, suggesting that the same rodent host can be infected with several pathogens at the same time, therefore, representing a serious threat to public health. Overall, this study provides important surveillance data on the prevalence of Leptospira spp. and hantaviruses from rodents in urbanized environment for the first time in Hungary and emphasizes the importance of further ecoepidemiological investigations.

As a result of discontinuing vaccination against smallpox after the late 1970s, different orthopoxviruses (OPVs), such as cowpox virus (CPXV), have become a re-emerging healthcare threat among zoonotic pathogens. In Hungary, data on OPV prevalence among its rodent host species have been absent. Here, rodents belonging to four species, i.e., striped field mouse (Apodemus agrarius), yellow-necked mouse (A. flavicollis), wood mouse (A. sylvaticus) and bank vole (Myodes glareolus), were live trapped at 13 sampling plots on a 149-ha area in the Mecsek Mountains, Hungary, from March to September in 2011 and 2012. Rodent sera were collected and screened for OPV-reactive antibodies with an immunfluorescence assay (IFA). Among the 1587 tested rodents, 286 (18.0%) harbored OPV-specific antibodies. Seroprevalence was the highest for the bank vole (71.4%) and the striped field mouse (66.7%). Due to a masting event in the autumn of 2011 across Central Europe, the abundance of bank voles increased drastically in the 2012 season, raising the overall OPV seroprevalence. We provide the first data on OPV occurrence and seroprevalence in rodents in Hungary. The circulation of OPV in rodents in densely populated areas warrants further studies to elucidate the zoonotic potential of OPV in humans.

Ecosystem/habitat restoration has become a major goal of international biodiversity policy. However, restorations are often limited in space or time, and we know little on whether and how restoration and management affect vertebrates. Here we assessed the local and landscape-scale effects of habitat restoration and management on small-mammal communities in the Egyek–Pusztakocs marsh system (Hortobagy National Park, Hungary), site of the largest active restoration of grasslands on former croplands in Europe. We live-trapped mice, voles and shrews in spring and autumn in 2 years (four sampling periods) at two sites in six habitat types: croplands, grasslands restored 3–6 years earlier and natural grasslands. Data on 421 individuals of 12 species showed that restored grasslands were similar to croplands and natural grasslands in species richness, abundance and composition. At the local scale, management influenced abundance because there were more small mammals in unmanaged and early-mown grasslands with taller vegetation than in late-mown or grazed grasslands with lower vegetation, or in ploughed croplands. Elevation was also important because sites at higher elevation provided refuges during spring floods or summer droughts. At the landscape scale, the proportion of restored and natural grasslands positively affected the abundance of small mammals, whereas the proportion of linear habitats (roads, canals) had a negative effect on abundance. Our results show that management is more important than restoration per se at the local scale, which is expected for habitat generalists such as small mammals in contrast to specialists such as plant-feeding invertebrates. However, restoration provides landscape-scale benefits by increasing the area of grasslands that can serve as refuges for small mammals in unfavourable periods. We thus conclude that a mosaic of restored and appropriately managed grasslands with tall vegetation will provide the best chances for the persistence of small-mammal communities in dynamic landscapes.

Crimean-Congo haemorrhagic fever virus (CCHFV) is a tick-borne pathogen, which causes an increasing number of severe infections in many parts of Africa, Asia and in Europe. The virus is primarily transmitted by ticks, however, the spectrum of natural hosts regarding CCHFV includes a wide variety of domestic and wild animals. Although the presence of CCHFV was hypothesized in Hungary, data in support of CCHFV prevalence has thus far, proven insufficient. In the present study, rodents belonging to four species, the yellow-necked mouse (Apodemus flavicollis), the striped field mouse (A. agrarius), the wood mouse (A. sylvaticus) and the bank vole (Myodes glareolus), were all systematically trapped in the Mecsek Mountain region (Southwest Hungary), from 2011 through 2013. Rodent sera were collected and screened for CCHFV antibodies with dot-blot pre-screening and immunofluorescence assay. Among the 2085 tested rodents, 20 (0.96%) were positive for IgG antibody against CCHFV. Seroprevalence was the highest (1.25%) in A. flavicollis serum samples. Distinctly, we now provide the first data regarding CCHFV occurrence and seroprevalence among wild rodents in Hungary. This observation represents a need for large-scale surveillance to effectively assess the enzootic background and the potential public health risk of CCHFV in Hungary.

The concept of nestedness has important role in community ecology of fragmented habitats that measures the order in presence-absence matrices of species in different communities, and the vulnerability of species to habitat change can be quantified. We have been examining the composition of small mammal assemblages of Kis-Balaton Landscape Protection Area since 1999 within the framework of Hungarian Biodiversity Monitoring System Programme. Our basic question was how much the species turnover processes and the water-level increase following bountiful precipitation as a natural disturbance predominate in directing the composition of small mammal assemblages and in the nestedness pattern in human disturbed habitat patches. Our investigation was based on nestedness analysis and the IndVal method. The results of nestedness analysis showed that the small mammal assemblages of the isolated Kis-Balaton marshland habitat patches are ordered contrary to the random pattern and showed nested pattern. The endangered habitat-specialist root vole appeared as an asymmetrical indicator species regarding its optimal habitat patch, and we evaluated it as an idiosyncratic species in the nestedness analysis. These results proved admittedly that the root vole – as a habitat specialist species – is very sensitive to habitat fragmentation and human disturbance, so the pattern of its presence influences considerably the nestedness of small mammal assemblages of the examined marshlands.

Movement dynamics of the striped field mouse were analysed on the basis of small mammal monitoring data gathered during a 10-year study period. The movement patterns were studied in relation to various forest and open mosaic habitats, in periods characterised with highly varying weather parameters. Our hypothesis i.e. the movement dynamics of the striped field mouse differ between habitats with different vegetation cover, was confirmed only for the drier, warm period when distances travelled by mouse individuals were highest in the mosaic grassland habitats. Data from the cooler and wetter period supported the alternative hypothesis i.e. there is no statistical difference between the habitats in their parameters of mouse dispersal movements.

In this study we investigated the role of histamine on the extramedullary hematopoiesis. Male histidine decarboxylase knockout (HDC−/−) mice and wild-type mice were used (n = 5/group). Groups of mice received sublethal total-body gamma irradiation at a single dose of 4 Gy. Spleen cells were studied at different time points post-irradiation by flow cytometry, colony forming unit (CFU) assay, and real-time PCR. For statistical analysis Student’s t test, ANOVA, and Holm-Sidak post-hoc test were used. By day 14 after irradiation, spleen cell counts increased almost eightfold in wild-type and not even fourfold in HDC−/− mice (P

Feeding habits and interspecific trophic niche overlap of two sympatric predators, the pine marten Martes martes and the red fox Vulpes vulpes, were studied in a deciduous forest habitat in Hungary with conditions of differing abundance and dominance in the rodent community. The main food source of the predators consisted of small mammals, mainly rodents. Consumption of small mammals was higher during bank vole Clethrionomys glareolus dominated years than in Apodemus mice dominated years. Both predators preferred bank voles as prey and consumed less Apodemus mice and shrews (Soricidae) than expected by availability. The two opportunistic predators utilised different, seasonally-dependent food resources. Martens consumed more plants, reptiles, amphibians and fish, whereas foxes consumed more small mammals and ungulate carcasses. In summer and autumn, percent biomass of bank voles in marten diet and Apodemus mice in fox diet was positively correlated with the number of rodents in the available food...

Plastic deformation of Al–Mg alloys were investigated by analyzing the stress–strain curves obtained in uniaxial compression tests at constant loading rate. Experimental results have shown the well-known phenomenon of plastic instabilities – the Portevin–Le Chatelier effect – in these solid solution alloys. Using the characteristics of plastic instabilities – the local stress increment, �σ , – dislocation densities were calculated and compared with those determined by considering the global strain hardening. The influence of the increasing Mg concentration on the multiplication and the annihilation of dislocations, as well as on the development of the mobile and immobile dislocation densities was also analyzed. © 2006 Elsevier B.V. All rights reserved.

The early stages of nucleation and growth of Guinier–Preston zones in Al –Zn –Mg and Al –Zn –Mg–Cu alloys are investigated by in situ synchrotron X-ray small-angle scattering. The in situ synchrotron measurements enable to follow the decomposition kinetics from about 2 minutes after quenching. Based on the measurement of the radii of gyration and integrated intensities, three distinct kinetic regimes are observed and discussed.

The protected European beaver, due to results of repatriation programmes, also spontaneously appeared in Hungary. These individuals may reached Hungary from the populations of neighbour countries (Austria, Croatia) by spreading. Occurrence of beaver was only detected in one point, on the watersmeet of Drava and Mura rivers, during monitoring the Somogy county wetlands of Danube-Drava National Park and only one inner-somogy occasion is known. The study is illustrated with 4 pictures.

Small mammal fauna monitoring in the Drava Lowlands has been done partly by barn owl pellet collecting and analysis. In the present study the correlations between landscape patterns and barn owl food composition (i.e. the indirect representation of small mammal communities) were analysed using an approach how the cummulated data of particular breeding pairs can be interpreted as characteristic for the patch composition of the mosaic landscape considerably larger than the hunting range of barn owls. For landscape ecological analysis CORTNE LANDCOVER 1:50.000 mapping categories were applied, based on which natural or semi-natural areas, differing from each other also in their barn owl hunting qualities, were differentiated. Three larger areas along river Drava were investigated: two in the upper reach monitoring zone (ZákányPorrogszentkirály, and Berzence-Heresznye), and one in the lower Drava reach (Drávaiványi-Szaporca). In the two upper-reach zones the localities differed from the greater scale landscape in less than 30% of the patches, with only 3-5 patches showing significant difference. In the lower reach there was one sampling locality with 6 patches differing from the greater-scale area, although in the entire lower section 33% or less difference was found between patch compositions of the two scales. Based on patch overlap calculations and homogeneity tests it was concluded that small mammal faunal data of the localities can be cumulated and can be evaluated on much larger landscape ecological scales than barn owl hunting ranges.

Small mammal monitoring using live trapping was done along the upper section of river Drava in 2003-2004, using capture-mark-recapture. Our sample areas were located in a strictly protected alder gallery forest (Lankóci-erdő) and in a neighbouring plot under gradual reforestation which had been clear-cut in 2000. A total of 9 small mammal species were recorded in the two sites, each comprising a 1-hectare sampling grid. The small mammal community revealed here had three characteristic rodent species (Apodemus flavicollis, A. agrárius, Clethrionomys glareolus), and two frequent shrew species (Sorex araneus, Crocidura leucodori). With the populations assumed to be closed, population sizes of the three dominant rodents were estimated from daily capture data. The highest estimated values were obtained for the two Apodemus populations (A. agrárius and A. flavicollis). Population sizes of the bank vole could be estimated from the protected area only. A spatial analysis with the nearest neighbour method showed that individuals of the more frequent species aimed at an even distribution, both in 2003 when densities were lower, and in 2004 when densities were higher. The more detailed analysis of the spatial pattern in 2004 suggested that by the end of late autumn, the different species are already organised in a pattern of winter survival strategy. It was shown by spatial distribution data that in this period the area of the closed alder gallery forest was occupied by individuals of yellow-necked wood mouse and bank vole. In the inner, larger part of the 1 ha forest section, it was mostly yellow-necked wood mice that established their home ranges. Striped field mice occupied only the reforested areas. Using extensive areas, the reforested area was inhabited also by bank voles, which fact is a proof for population translocation between the two areas in this species.

The stress–strain relationships of high-purity aluminum and copper were investigated over a wide range of strain by combining data obtained in the conventional tensile and compression testing of annealed samples with data obtained after processing by equal-channel angular pressing (ECAP) to high imposed strains. The true stress–true strain curves were analyzed mathematically taking the absolute strain as zero for the annealed condition and equal to the strain imposed through ECAP for the as-pressed samples. It is shown that, over the entire range of strain inherent in these experiments, the macroscopic stress–strain behavior of Al and Cu may be represented by an exponential power-law constitutive relationship which reduces to the conventional Hollomon power-law relationship at low strains and to the Voce exponential relationship at high strains. It is demonstrated that the results obtained from tensile or compressive testing to low strains at room temperature are sufficient, when used with the new constitutive relationship, to provide detailed information on the nature of the stress–strain behavior to high strains. The validity of the new relationship is supported by theoretical considerations which incorporate the major micro-mechanisms of plastic deformation.

Knowledge of the acute and late ionizing radiation exposure damage to the gastrointestinal tract, particularly injury of the small intestine, is of great significance in radiotherapy, as is management of accidental radiation exposure. Irradiation (X-ray, neutron, cobalt gamma) induces a series of events in this rapidly renewing tissue resulting in the well-known symptoms of the gastrointestinal (GI) radiation syndrome, such as GI haemorrhage, endotoxemia, bacterial infection, anorexia, nausea, vomiting, diarrhoea, and loss of electrolytes and fluid. In spite of the significant advances that have occurred in research on underlying mechanisms over the last two decades, the overall etiology and pathogenesis of the GI-syndrome still remains unclear. Currently, to our knowledge, these symptoms are probably due to a rapid modification of the intestinal motility and to the structural alteration of the intestinal mucosa (cell loss and altered crypt integrity). Several evidences suggest that radiation-induced dysfunctions and structural changes of this organ (either changes in subcellular, cellular, and histological structure) are mediated by concerted and interrelated changes of a plethora of various extracellular mediators and their intracellular messengers. The aim of this review is to summarize our current knowledge about the pathomorphology and cell biology of the ionizing radiation response of the GI tract with a focus on the small intestine.

Concentration of the radionuclide 226Ra was determined in almost every type of bottled mineral water commercially available in Hungary. Determination of the radon coming from the radium dissolved in the water was used for activity measurement. As the results show, the 226Ra concentrations exceed the level of 100 mBq l(-1) in six cases out of the 28 types of mineral water investigated. In one case 3 Bq l(-1) was measured, which provides 0.3 mSv year(-1) committed effective dose for adults in the case of a consumption rate of 1 l day(-1). In soft drinks produced from mineral water a concentration of 2.6 Bq l(-1) was determined, which means 1.4 mSv year(-1) effective dose in the age group 12-17 years in the case of permanent daily drinking of 1 l of these beverages.

Among the Hantavirus genus, Saaremaa virus (SAAV) has been the subject of taxonomical debates. While the International Committee on Taxonomy of Viruses declares SAAV as a distinct species, several European hantavirus experts proposed that SAAV is in fact a genotype of Dobrava-Belgrade virus (DOBV). In the present study we performed S-segment-based phylogenetic analysis of eight DOBV strains identified in rodents in Hungary and Northern Croatia. These new sequences considerably increase the number of complete nucleoprotein gene sequences deposited in the NCBI database. Our phylogenetic analysis clearly support the taxonomical nomenclature recently proposed for DOBV, i.e., genotypes such as Dobrava, Saaremaa, Kurkino, and Sochi should indeed be classified within the DOBV hantavirus species. Moreover, we found that only the Dobrava and Kurkino genotypes of DOBV species are circulating in Hungary while currently there is no evidence for the presence of Saaremaa genotype.

A frequently used method in determining the radium concentration of water is the radon emanation method. When radon gas is transferred to the Lucas-cell usually CaCl2 is used to remove the water traces. When we measured the background of the system using ultra clear distilled water the results were astonishing. The detailed investigation has shown that the unwanted radon originated from the CaCl2, contained about 1000 Bq/kg of 226Ra. Depending on the time interval between two measurement, the radon deriving from the CaCl2 disturbed the measurements.

Dobrava (DOBV) hantaviruses belong to the genus Hantavirus, family Bunyaviridae, and are carried by yellow-necked and striped field mice. The goal of this study was to detect DOBV using serological and genetic methods in Apodemus rodents in Hungary and in northern Croatia. During the study period, a total of 125 Apodemus sp. (67 A. agrarius, 58 A. flavicollis) were tested for the presence of hantaviruses, and 21 rodents (17%) were positive by rRT-PCR and/or ELISA. We conclude that the prevalence of DOBV is much higher than previously anticipated. The simultaneous use of molecular and serological techniques provides a highly reliable way to detect hantavirus infections.

The action of the tripeptide aldehyde t -butyloxycarbonyl-DPhe-Pro-Arg-H (boc-fPR-H), belonging to a family of serine proteinase inhibitors, on the release of immunoreactive prolactin (iPRL) and growth hormone (iGH) has been studied. In rat anterior pituitary cell cultures and pituitary quarters 1 mM boc-fPR-H inhibited basal iPRL and iGH release. Thyroliberin-induced iPRL release by cultured cells was also markedly inhibited with a concomitant accumulation of intracellular iPRL. During the short- and long-term exposure of cells to boc-fPR-H there were no changes in total cell protein contents and in activities of some lysosomal marker enzymes. A wide scale of unchanged parameters characteristic for cellular metabolism indicated that the tripeptide aldehyde has no cytotoxic effect. The marked inhibition of basal as well as stimulated hormone release in the presence of the enzyme inhibitor might suggest that at least a portion of the hormones is released via a proteolytic enzyme-dependent process.

The effects of novel nontoxic tripeptide aldehyde inhibitors of proteolytic enzymes were examined in order to investigate the possibility that serine-thiol protease(s) may be involved in PRL and GH secretion. Rat anterior pituitary cells maintained in culture for 7-8 days or freshly taken pituitary quarters were treated with BOC-DPhe-Pro-Arg-H (BOC-dPPA), DPhe-Pro-Arg-H (dPPA), BOC-DPhe-Leu-Lys-H (BOC-dPLL), or BOC-DPhe-Phe-Lys (BOC-dPPL). Newly synthetized [3H]PRL and [3H]GH as well as immunoreactive (i) hormones (iPRL, iGH) were measured in the incubation media and cell homogenates. Four hours of incubation in the presence of 0.1 mM dPPA resulted in a 30% decrease of [3H]PRL and iPRL release by cell cultures; the inhibition by BOC-dPPA was 60% and 48%, respectively. [3H]PRL biosynthesis was unchanged or slightly decreased. The effect of these tripeptide aldehydes on [3H]GH and iGH release was less pronounced but statistically significant. Pituitary quarters treated with 1.0 or 3.0 mM BOC-dPPA release 20% and 57% less [3H]PRL than the controls. In the same system BOC-dPPA in a 1.0 mM concentration did not effect GH secretion, and 3.0 mM BOC-dPPA inhibited [3H]GH output by 27%. Forty micromolars of BOC-dPPL decreased by 47%, 0.2 mM by 79%, and 1.0 mM by 94% [3H]PRL release from pituitary quarters. GH secretion was not influenced. A similar selectivity was observed when BOC-dPLL was used. It is clear that by serine-thiol protease inhibitors whose effects are sequence and dose dependent, PRL and GH release are decreased. The relative inhibiting potency on PRL release was BOC-dPPL greater than BOC-dPLL greater than BOC-dPPA greater than dPPA. The biosynthesis of [3H]PRL was reduced only in the presence of the highest tripeptide aldehyde concentrations or long (8 h) exposure, and only 1.0 mM Boc-dPPL reduced [3H]GH biosynthesis by 30%. The data suggest that proteolysis may be involved in the process of PRL and GH release and the enzyme(s) in question may be serine-thiol protease(s).

The degree of endocrine activity by rat pituitary lactotrophs was manipulated and the lysosomal involvement in the release and intracellular degradation of PRL was monitored by concomitant assays of acid phosphatase, beta-glucuronidase, dipeptidyl peptidases I and II, and nonspecific esterases in the anterior lobe. The in vitro release of PRL by cell cultures was inhibited by 0.5 or 1.5 microM bromocriptine during 24 or 72 h of incubation and by 0.75 microM for 4 h. Long term treatment caused a 40% reduction in the total PRL content of cells and media; however, after 4 h of bromocriptine treatment, no reduction in PRL content was found. TRH (10 ng/ml) in medium for 4 h increased PRL release, whereas it produced intracellular hormone depletion. In vitro treatment of anterior lobes of diestrous rats with 1 microM dopamine decreased PRL release by 50%. No changes in lysosomal enzyme activities were observed after the inhibition of release or stimulation of the in vitro secretion of PRL. Haloperidol (2.5 mg/kg BW) caused a 90% increase in serum PRL concentrations in diestrous rats 1 h after sc injection. alpha-Methyl-p-tyrosine (200 mg/kg BW, ip) stimulated in vivo PRL secretion, monitored 1, 4, and 24 h after drug administration. The administration of 100 micrograms/rat bromocriptine for 3 consecutive days, a single dose of 100 micrograms/rat polyestradiol phosphate, and their combination resulted in the expected changes in PRL production in female rats. These in vivo treatments failed to alter lysosomal enzyme activities in the anterior pituitary. These findings suggest that the release, intracellular accumulation, or depletion of PRL occurred without concomitant changes in lysosomal enzyme activity in the anterior pituitary. We suggest that the system of lysosome-dependent hormone degradation may involve more specific enzymes than those monitored to date.