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Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine

Authors :
Henrietta Papp
Mónika Madai
Fanni Földes
Gábor Kemenesi
Róbert Herczeg
Brigitta Zana
Kornélia Kurucz
Győző Horváth
Balázs Somogyi
Safia Zeghbib
Ferenc Jakab
Source :
Viruses, Vol 13, Iss 570, p 570 (2021), Viruses
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

The natural hosts of Orthohantaviruses are rodents, soricomorphs and bats, and it is well known that they may cause serious or even fatal diseases among humans worldwide. The virus is persistent among animals and it is shed via urine, saliva and feces throughout the entirety of their lives. We aim to identify the effectiveness of hantavirus detection in rodent tissue samples and urine originating from naturally infected rodents. Initially, animals were trapped at five distinct locations throughout the Transdanubian region in Hungary. Lung, liver, kidney and urine samples were obtained from 163 deceased animals. All organs and urine were tested using nested reverse transcription polymerase chain reaction (nRT-PCR). Furthermore, sera were examined for IgG antibodies against Dobrava–Belgrade virus (DOBV) and Puumala virus (PUUV) by Western blot assay. IgG antibodies against hantaviruses and/or nucleic acid were detected in 25 (15.3%) cases. Among Apodemus, Myodes, and Microtus rodent species, DOBV, PUUV and Tula virus (TULV) were clearly identified. Amid the PCR-positive samples, the nucleic acid of the viruses was detected most effectively in the kidney (100%), while only 55% of screened lung tissues were positive. Interestingly, only three out of 20 rodent urine samples were positive when tested using nRT-PCR. Moreover, five rodents were seropositive without detectable virus nucleic acid in any of the tested organs.

Details

Language :
English
ISSN :
19994915
Volume :
13
Issue :
570
Database :
OpenAIRE
Journal :
Viruses
Accession number :
edsair.doi.dedup.....5b13e0d556d599e3d391ffbff798cfb1