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Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine
- Source :
- Viruses, Vol 13, Iss 570, p 570 (2021), Viruses
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- The natural hosts of Orthohantaviruses are rodents, soricomorphs and bats, and it is well known that they may cause serious or even fatal diseases among humans worldwide. The virus is persistent among animals and it is shed via urine, saliva and feces throughout the entirety of their lives. We aim to identify the effectiveness of hantavirus detection in rodent tissue samples and urine originating from naturally infected rodents. Initially, animals were trapped at five distinct locations throughout the Transdanubian region in Hungary. Lung, liver, kidney and urine samples were obtained from 163 deceased animals. All organs and urine were tested using nested reverse transcription polymerase chain reaction (nRT-PCR). Furthermore, sera were examined for IgG antibodies against Dobrava–Belgrade virus (DOBV) and Puumala virus (PUUV) by Western blot assay. IgG antibodies against hantaviruses and/or nucleic acid were detected in 25 (15.3%) cases. Among Apodemus, Myodes, and Microtus rodent species, DOBV, PUUV and Tula virus (TULV) were clearly identified. Amid the PCR-positive samples, the nucleic acid of the viruses was detected most effectively in the kidney (100%), while only 55% of screened lung tissues were positive. Interestingly, only three out of 20 rodent urine samples were positive when tested using nRT-PCR. Moreover, five rodents were seropositive without detectable virus nucleic acid in any of the tested organs.
- Subjects :
- 0301 basic medicine
Orthohantavirus
Hantavirus Infections
030106 microbiology
lcsh:QR1-502
Rodentia
Urine
Antibodies, Viral
Kidney
Virus
lcsh:Microbiology
03 medical and health sciences
Virology
anatomy_morphology
Animals
Lung
Feces
Hantavirus
Disease Reservoirs
Hungary
biology
hantavirus detection
Communication
Histological Techniques
rodent
tissue
biology.organism_classification
urine
Reverse transcription polymerase chain reaction
naturally infected
030104 developmental biology
Infectious Diseases
Liver
biology.protein
RNA, Viral
Puumala virus
Antibody
Tula virus
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Volume :
- 13
- Issue :
- 570
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....5b13e0d556d599e3d391ffbff798cfb1