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MicroRNAs (miRNAs) are implicated in the development of cancers and may serve as potential targets for therapy. However, the functions and underlying mechanisms of miRNAs in cancers are not well understood. This work aims to study the role of miR-373-3p in colon cancer cells. We find that the expression of miR-373-3p mimics promotes and the miR-373-3p inhibitor suppresses aerobic glycolysis and proliferation of colon cancer cells. Mechanistically, miR-373-3p inhibits the expression of MFN2, a gene that is known to suppress glycolysis, which leads to the activation of glycolysis and eventually the proliferation of cells. In a nude mouse tumor model, the expression of miR-373-3p in colon cancer cells promotes tumor growth by enhancing lactate formation, which is inhibited by the co-expression of MFN2 in the cells. Administration of the miR-373-3p antagomir blunts in vivo tumor growth by decreasing lactate production. In addition, in human colon cancers, the expression levels of miR-373-3p are increased, while those of MFN2 mRNA are decreased, and the increase of miR-373-3p is associated with the decrease of MFN2 mRNA. Our results reveal a previously unknown function and underlying mechanism of miR-373-3p in the regulation of glycolysis and proliferation in cancer cells and underscore the potential of targeting miR-373-3p for colon cancer treatment.

With the application of shrouds made of silicon carbide ceramic matrix composite（SiC-CMCs），the abradable coating technology suitable for SiC-CMCs is urgently needed. The four-layer structure of BSAS（Ba0.75Sr0.25Al2Si2O8）-polyester-based abradable environmental barrier coating （A/EBCs） was prepared by atmospheric plasma spraying. The influence of process parameters on the porosity and the structural evolution of the layer at 1300 ℃ were investigated. The phase structure，microstructure and coating composition were characterized by XRD，SEM，EDS and TEM respectively. The results show that the porosity of BSAS-polyester abradable top layer is 26.4%-36.8%. And the temperature-sensitive parameters of BSAS-polyester particles are primary gas （argon） flow，second gas （hydrogen） flow and spraying distance. In contrast，the speed-sensitive parameters are primary gas （argon） flow. The primary gas （argon） flow has the opposite effect on the particle temperature and velocity of the BSAS-polyester. The abradable surface layer is oxidized at 1300 ℃ for 300 h and maintains monoclinic phase structure，stable stucture and composition，and locally precipitates spherical amorphous silicon oxide particles. Furthermore，the coating abradability was evaluated by high-temperature and high-speed scraping test. Nano-scale Ni-based superalloy particles adhesion on the surface of the coating are found，and the blade incursion depth ratio （IDR） is 20%，which meets the requirements of the abradable sealing coating.

In order to expand the application of polypropylene (PP) in 3D printing technology and improve its mechanical properties, PP/polyethylene terephthalate (PET) blend filaments were prepared by extruding PP with PET using melt blending. The rheological behavior, crystallization structure, melting and crystallization behavior, microstructure, mechanical properties of the filament blends and 3D printed samples with different mixing ratios of PP, PET were analyzed. The results show that the viscosity of PP/PET blends decreases with the increase of shear rate and exhibits shear thinning behavior. PET acts as a nucleating agent and does not change the crystal shape of PP during PP crystallization. The melting temperature of PP is about 150 ℃, and the crystallization temperature increases with the increase of PET concentrations. The PP matrix and PET dispersed phase have obvious "sea-island" structure, in which PET is the "island" phase, with a diameter of 2-5 μm. With the increase of PET concentrations, the tensile strength of filament blends is improved. Simultaneously, the tensile strength and the impact strength of the 3D printed samples is improved. However, the mechanical properties of 3D printed samples are generally lower than those of filament blends due to interlayer interaction.

C16H20MnN10O8, monoclinic, P21/c (no. 14), a = 11.1178(7) Å, b = 12.7838(6) Å, c = 15.6649(10) Å, β = 105.692(6)°, V = 2143.4(2) Å3, Z = 4, Rgt(F) = 0.0638, ωRref(F2) = 0.2034, T = 293 K.

Background: To explore the optimal dosage of heparin in peripheral arteriovenous automatic synchronous exchange transfusion therapy for neonatal hyperbilirubinemia. Methods: A total of 185 neonates received peripheral arteriovenous synchronous blood exchange transfusion for hyperbilirubinemia were enrolled from pediatric department of the Ganzhou People's Hospital between January 2018 and June 2020, which were randomly divided into four groups. On the basis of exchange transfusion, different dose of heparin was pumping at the bleeding site of artery (A: no heparin; B: 100 U/h heparin; C: 200 U/h heparin; D: 300 U/h heparin). The indexes of exchange transfusion efficacy, including total bilirubin conversion rate, indirect bilirubin conversion rate, hemoglobin concentration, the platelet number and APTT value was measured before and after therapy. The sites of artery puncture, the sites and rate of vascular occlusion were counted and analyzed. Results: There was no significant difference for total bilirubin conversion rate, indirect bilirubin conversion rate, hemoglobin concentration platelet number and APTT value before and after exchange transfusion between the four groups (P >0.05). In addition, there was no statistical significance in artery puncture sites between the four groups (P > 0.05). Significant difference in total rate of vascular occlusion and rate of radial artery occlusion were found between the four groups (P< 0.05). Conclusion: Different dose of heparin using in peripheral arteriovenous synchronous blood exchange transfusion did not affect the therapeutic effect or coagulation function of patients, but could reduce the rate of occlusion depending by the site of arterial puncture.

Prolyl hydroxylase 3 (PHD3) hydroxylates HIFα in the presence of oxygen, leading to HIFα degradation. PHD3 inhibits tumorigenesis. However, the underlying mechanism is not well understood. Herein, we demonstrate that PHD3 inhibits the metastasis of colon cancer cells through the occludin-p38 MAPK pathway independent of its hydroxylase activity. We find that PHD3 inhibits colon cancer cell metastasis in the presence of the PHD inhibitor DMOG, and prolyl hydroxylase-deficient PHD3(H196A) suppresses cell metastasis as well. PHD3 controls the stability of the tight junction protein occludin in a hydroxylase-independent manner. We further find that PHD3-inhibited colon cancer cell metastasis is rescued by knockdown of occludin and that occludin acts as a negative regulator of cell metastasis, implying that PHD3 suppresses metastasis through occludin. Furthermore, knockdown of occludin induces phosphorylation of p38 MAPK, and the p38 inhibitor SB203580 impedes cell migration and invasion induced by occludin knockdown, indicating that occludin functions through p38. Moreover, knockdown of occludin enhances the expression of MKK3/6, the upstream kinase of p38, while overexpression of occludin decreases its expression. Our results suggest that PHD3 inhibits the metastasis of colon cancer cells through the occludin-p38 pathway independent of its hydroxylase activity. These findings reveal a previously undiscovered mechanism underlying the regulation of cancer cell metastasis by PHD3 and highlight a noncanonical hydroxylase-independent function of PHD3 in the suppression of cancer cells.

This paper summarized the treatment and nursing measures for a patient with limb pain caused by arteriosclerosis obliterans. Based on the nursing risk assessment and routine nursing, the intradermal needling and digital acupoint pressure therapy were adopted to relieve the limb pain caused by arteriosclerosis obliterans, improve the patient’s quality of life and rehabilitation. (本文回顾1例闭塞性动脉硬化症患者肢体疼痛的治疗和护理体会。在完善护理评估的和常规护理的基础上, 运用中医特色护理技术皮内针联合手指点穴治疗, 能够减轻患者肢体疼痛, 提高生活质量, 促进康复。)

C6H11N3O3, monoclinic, P21/n (no. 14), a = 6.1802(3) Å, b = 12.6623(5) Å, c = 10.0897(4) Å, β = 93.328(2)°, V = 760.10(6) Å3, Z = 4, Rgt(F) = 0.0341, wRref(F2) = 0.0864, T = 150 K.

Shot peening (SP) process is a typical surface strengthening process for metal and metal matrix composites, which can significantly improve the fatigue life and strength. The traditional SP simulation model falls short as it only takes into account one or a few shots, proving insufficient for accurately simulating the entire impact process involving hundreds of shots. In this study, a random multiple shots simulation modeling methodology with hundreds of random shots is proposed to simulate the impact process of SP. In order to reduce the simulation error, the random function Rand of MATLAB is used to generate the shot distributions many times, and the shot distribution closest to the average number is selected and the three-dimension parametric explicit dynamics numerical simulation model is built using ABAQUS software. Orthogonal experiments are carried out to investigate the influences of shot diameter, incident impact velocity, and angle on the residual stress distribution, roughness, and specimen deformation. Results showed that the average relative errors of maximum residual compressive stress, roughness, and deformation of specimen between simulation model and experimental value are 30.99, 16.14, and 16.73%, respectively. The primary factors affecting residual stress and deformation is shot diameter, and the main factor affecting roughness is impact velocity.

Objective: This study aimed to realize rapid on-site screening of enrofloxacin in eggs. Methods: According to the requirements of the “Maximum residue limit of 41 veterinary drugs in the national food safety standard” and the “Opinions on regulating the use of rapid food testing” issued by the State Market Supervision and Administration in January 2023, the spiked samples at three concentration levels of enrofloxacin were prepared. Fifty blank samples and fifty spiked samples at a concentration level of 0.5 time the standard limit requirement (5 μg/kg) were used to evaluate the false positive rate of the rapid detection method. Fifty spiked samples at a concentration level of 1 time the standard limit requirement (10 μg/kg) were used to evaluate the false negative rate of the rapid detection method. Fifty real samples of eggs were detected with immunochromatographic test strips based on quantum dot microsphere and HPLC-MS/MS, respectively. Results: The immunochromatographic test strips based on quantum dot microsphere can meet the national limit requirements, with false negative rate of 0% and false positive rate of 0%. For the detection with real samples, the results of the immunochromatographic test strips based on quantum dot microsphere have a high coincidence rate with that of HPLC-MS/MS. Conclusion: With the help of a portable reader, quantum-dot microsphere immunochromatography can obtain digital and accurate results, which is suitable for rapid on-site screening of large samples of risk factors in eggs.

This paper summarized the nursing of integrated Traditional Chinese and western medicine for a patient with ankylosing spondylitis(AS) complicated with ecthyma. Based on the Traditional Chinese Medicine syndrome differentiation, TCM-characterized nursing technology such as Chinese Medicine collapse stains, moxibustion, acupoint massage, auricular acupoint sticking and pressing, dietary guidance were used jointly. Implementation of integrative nursing is potentially effective to relieve the pain and improve the ulcer healing. (本文总结1例强直性脊柱炎并发臁疮患者的中西医结合护理措施。在常规护理的基础上辨证施护, 合理应用中药塌渍、艾灸、穴位按摩、耳穴贴压、膳食指导等中医特色护理技术, 能有效缓解患者疼痛, 促进溃疡的早期愈合。)

Despite the ongoing debate on the inconsistent and controversial effects of Tibetan diet on blood lipid levels at high altitude, this cross-sectional study was conducted to analyze the relationship between dietary practices and blood lipid levels among Jiarong Tibetan population. A total of 476 Jiarong Tibetan residents were included, in which basic demographic data, physical activity records, simplified food frequency questionnaire, and biochemical data were collected. Using multivariate logistic regression analysis, the potential associations between the variables were examined, and it was found that fat energy supply ratio increased with the elevation of altitude, while the lipid level showed an inverted U-shaped variation. However, the findings suggested that a diet rich in unsaturated fatty acids might balance the effects of the Tibetan diet on the risk of lipid metabolism disorders. Therefore, it is crucial to concentrate on the fat composition rather than the amount of fat E% intake on the plateau. The results highlighted the importance of investigating the interaction between environment and genes in lipid levels among plateau Tibetan population. However, further large-scale prospective studies are required for better understanding of the complexities involved in dietary practices and their influences on blood lipid levels.

Objective To investigate the effects of integrated Traditional Chinese and Western medicine nursing on treatment outcome, quality of life and prognosis of high-risk patients with diabetic foot, and provide evidence for more reasonable nursing intervention program. Methods From January 2021to January 2022, 200 patients with type 2 diabetes mellitus were screened; 40 high-risk patients with diabetic foot were eligible and randomly divided in to observation group and control group, with 20 cases in each group. The control group received routine care during the treatment, and those in the observation group received integrated traditional Chinese and Western medicine intervention based on routine care. The treatment outcome, patient quality of life and prognosis of disease were compared between two groups. Results The overall effective rate in the observation group was higher than that in the control group (P＜0. 05). According to the outcome of General Quality of Life Inventory-74(GQOL-74) assessment, the improvement rate in the observation group was 85. 00%(17/20), showing no significant difference compared with 65. 00%(13/20) in the control group (P＞0. 05). The incidences of foot infections and foot ulcers in the observation group were lower than those in the control group (P＜0. 05). Three cases of amputation were reported in the control group. Conclusion The integrated Traditional Chinese and Western medicine nursing showed positive effect on treatment outcome, patient quality of life and prognosis of disease, and the effect of integrative nursing intervention was superior to routine nursing. (目的 观察中西医结合护理干预高危糖尿病足患者的效果, 及对患者生活质量及预后的影响, 为高危糖尿病足患者采取更好的护理策略、制定更为合理的护理干预方案提供依据。方法 从医院2021年1月—2022年1月收治的200例2型糖尿病患者中筛查出40例高危糖尿病足患者, 随机分为现察组与对照组, 各20例。对照组采取常规护理干预, 观察组在常规护理基础上, 给予中西医结合护理干预。评价两组干预效果, 以及对患者生活质量以及足部状况影响。结果 观察组干预效果优于对照组, 差异有统计学意义(P＜0. 05); 观察组生活质量评定问卷(GQOL-74)评分改善率85. 00%(17/20), 高于对照组的65. 00%(13/20), 但差异无统计学意义(P＞0. 05); 观察组足部感染和足部溃疡发生率低于对照组, 差异有统计学意义(P＜0. 05), 观察组无1例患者截肢, 对照组3例患者截肢。结论 中西医结合护理干预能进一步提高高危糖尿病足患者的治疗效果, 改善患者生活质量及预后, 干预效果要优于常规护理干预。)

Objective To explore the effect of lncRNA FGD5-AS1 on oxidized low-density lipoprotein (ox-LDL)-induced vascular endothelial cell damage and its regulatory effect on miR-106a-5p. Methods Ox-LDL was used to induce human umbilical vein endothelial cells(HUVECs) to establish a cellular injury model. pcDNA-FGD5-AS1, anti-miR-106a-5p and its negative control were transfected into HUVECs and then added to ox-LDL-treated cells. pcDNA-FGD5-AS1 was co-transfected with miR-NC and miR-106a-5p mimics to HUVECs, then added ox-LDL to treat cells. RT-qPCR was used to detect the expression of FGD5-AS1 and miR-106a-5p. The level of MDA, SOD, and CAT was tested with commercially available kit. Flow cytometry was used to detect the apoptosis rate. The dual luciferase reporter gene experiment was used to detect the targeting relationship between FGD5-AS1 and miR-106a-5p. Western blot was used to detect the protein expression of cleaved caspase-3 and cleaved caspase-9. Results The expression of FGD5-AS1 mRNA in HUVECs induced by ox-LDL decreased (P＜0.05) while the expression of miR-106a-5p mRNA increased (P＜0.05). Both transfection of pcDNA-FGD5-AS1 and transfection of anti-miR-106a-5p reduced the level of MDA, apoptosis rate and protein level of cleaved caspase-3, cleaved caspase-9 in HUVECs induced by ox-LDL(P＜0.05),enhanced the activity of SOD and CAT (P＜0.05). FGD5-AS1 could target at miR-106a-5p. Co-transfection of pcDNA-FGD5-AS1 and miR-106a-5p mimics could reverse the effect of pcDNA-FGD5-AS1 on ox-LDL-induced HUVECs apoptosis and oxidative stress. Conclusions Over-expression of FGD5-AS1 may inhibit oxidative stress and apoptosis through targeted regulation of miR-106a-5p, thereby reducing ox-LDL-induced vascular endothelial cell damage.

There is a problem about microstructure evolution and properties degradation for the superalloy turbine blades in long term service conditions. DZ406 alloy samples were pre-loaded to simulate the high temperature service environment of turbine blades. The thermodynamic coupling simulation conditions were 980 ℃/70 MPa, 980 ℃/110 MPa, 980 ℃/140 MPa and 980 ℃/180 MPa respectively. And then the samples were subjected to stress rupture property test at 980 ℃/275 MPa. The microstructure and 980 ℃/275 MPa rupture life of the samples under different service loading conditions were observed and analyzed. The results show that the heat treatment microstructure of DZ406 alloy is composed of carbides, residual γ +γ´ eutectic and regular γ´ phase. The morphology and size of carbides and eutectic have no obvious change with the increase of loading stress under simulated service conditions. The γ´ phase of the sample parallel to [001] direction presents different degrees of rafting, and the size of γ´ phase perpendicular to [001] direction obviously increases. The residual stress rupture life of the sample declines rapidly with the increase of service stress.

This paper explore the construction and practice of nursing service system in the Traditional Chinese Medicine (TCM) appropriate technology clinic. TCM appropriate technology clinic, as an independent nursing unit, explores the management mode, hardware facilities and equipment configuration and environment construction, nursing personnel configuration, service items, quality control, and cooperative departments, and develops the nursing service system. The construction and application of TCM appropriate technology clinic nursing service system has expanded the service scope for nursing personnel to implement TCM appropriate technology, met the needs of outpatients for TCM appropriate technology, promoted the improvement of TCM nursing service capacity, and provided reference for the construction of TCM nursing outpatient service system. (本文探讨了中医适宜技术门诊护理服务体系的构建及应用效果。中医适宜技术门诊作为独立的护理单元, 从管理模式、诊室硬件设施设备配置及环境建设、护理人员配置、服务项目、质量监控、合作科室6个方面进行探索, 制定中医适宜技术门诊护理服务体系。医院中医适宜技术门诊护理服务体系的构建和应用, 拓展了护理人员实施中医适宜技术的服务范围, 满足了门诊患者对中医适宜技术的需求, 促进了中医护理服务能力的提升, 可为中医护理门诊服务体系建设提供参考。)

Objective To observe the effect of moxibustion on pain relief in patients with lower extremity arteriosclerosis obliterans (ASO)of cold coagulation and blood stasis type. Methods Totally 60 patients with lower extremity ASO of cold coagulation and blood stasis type were randomly divided into the control group and the observation group, with 30 cases in each group. Patients in both groups received routine care during the hospitalization, and those in the observation group were given moxibustion by Traditional Chinese Medicine (TCM) syndrome differentiation. The Visual Analogue Scale (VAS)was used to assess the degree of pain. Results The VAS score of the two groups decreased after nursing intervention, and VAS score in the observation group was lower than that in the control group (P＜0. 01). Conclusion The moxibustion by TCM syndrome differentiation is effective to relieve the pain of patients with lower extremity ASO of cold coagulation and blood stasis type. (目的 观察艾灸缓解下肢动脉硬化性闭塞症寒凝血瘀型患者疼痛的效果。方法 60例下肢动脉硬化性闭塞症寒凝血瘀型患者随机分为对照组和观察组, 各30例。对照组给予常规护理, 观察组在常规护理基础上增加中医护理干预, 根据中医辨证分型实施艾灸。采用视觉模拟评分(VAS)评估治疗前后患者疼痛程度。结果 干预后两组VAS评分均较干预前下降, 且观察组VAS评分低于对照组, 差异有统计学意义(P＜0. 01)。结论 基于中医辨证分型实施艾灸, 能进一步缓解下肢动脉硬化性闭塞症寒凝血瘀型患者疼痛。)

Morchella is a genus of fungi with the ability to concentrate Cd both in the fruit-body and mycelium. However, the molecular mechanisms conferring resistance to Cd stress in Morchella are unknown. Here, RNA-based transcriptomic sequencing was used to identify the genes and pathways involved in Cd tolerance in Morchella spongiola. 7444 differentially expressed genes (DEGs) were identified by cultivating M. spongiola in media containing 0.15, 0.90, or 1.50 mg/L Cd2+. The DEGs were divided into six sub-clusters based on their global expression profiles. GO enrichment analysis indicated that numerous DEGs were associated with catalytic activity, cell cycle control, and the ribosome. KEGG enrichment analysis showed that the main pathways under Cd stress were MAPK signaling, oxidative phosphorylation, pyruvate metabolism, and propanoate metabolism. In addition, several DEGs encoding ion transporters, enzymatic/non-enzymatic antioxidants, and transcription factors were identified. Based on these results, a preliminary gene regulatory network was firstly proposed to illustrate the molecular mechanisms of Cd detoxification in M. spongiola. These results provide valuable insights into the Cd tolerance mechanism of M. spongiola and constitute a robust foundation for further studies on detoxification mechanisms in macrofungi that could potentially lead to the development of new and improved fungal bioremediation strategies.

Pit-1 (POU1F1) is a POU-homeodomain transcription factor, and it is one of the most important tissue-specific transcription factors in pituitary development. Cyclin-dependent kinase 5 (CDK5) is a protein kinase that can phosphorylate many key transcription factors, but the mechanism under which CDK5 phosphorylates Pit-1 is unclear. To investigate whether CDK5 can regulate cell proliferation and promote hormone secretion through phosphorylation of Ser126-Pit-1 in prolactinomas, we generated an antibody that specifically recognizes phosphorylated serine at position 126 of Pit-1 (Ser126-Pit-1). We used western blotting to detect the level of Pit-1 phosphorylation and observed the proliferation and apoptosis of GH3 cells with different levels of Pit-1 phosphorylation by clone formation experiments, cell viability assays, and flow cytometry. ELISA was used to measure the level of PRL in the supernatant of GH3 cells. Tissue microarrays and immunohistochemistry were used to evaluate the expression of the phosphorylation level of Ser126-Pit-1 (pSer126-Pit-1) in prolactinomas. Our data indicated that Ser126-Pit-1 is specifically phosphorylated by CDK5 and high-level pSer-126-Pit-1 can promote cell proliferation and PRL secretion. In addition, a higher level of pSer-126-Pit-1 correlates with a worse prognosis in patients with prolactinoma. Our results show that CDK5 mediated Ser126-Pit-1 phosphorylation and regulated prolactinoma progression and PRL secretion.

[Purpose/significance] There is a rather complete library service network and talent cultivating system in India, and its experience and practice in the construction of reading service courses would be of great reference value for our work in this field. [Method/process] This paperfirstly visited the websites of the institutions engaged in library and information science education in India, then did case study on the courses of reading service. [Result/conclusion] It is found that there are two universities in India that systematically offer three reading service courses, which cover two representative service groups of library, i.e., children and adults. The content of each course is comprehensive, by studying it students will receive a systematic training in the library reading service theory and methods. There are three features with India's reading service courses: highly targeted, emphasizing the cultivation of students' practical ability and providing flexible online education, which can better meet the large demand for reading service talents in India and is worthy of reference in China. Three lessons can be drawn from the experience of India: to design curriculum system that cover the primary reading service librarian and target audience; to design and implement effective modular internship programs; to offer certificate courses over the Internet.

Polylactic acid (PLA) is a widely used biopolymer material. However, there are disadvantages such as poor toughness, poor hydrophilicity, and poor biological activity in the application process. It was modified with polyethylene glycol (PEG) and hydroxyapatite (HA).3D printing filaments of PLA/PEG/HA with different mass ratios were prepared by melt blending. And by analyzing the mechanical properties, crystallization properties, thermal properties, rheological properties of PLA/PEG/HA filaments, the more suitable filaments for fused deposition modeling of 3D printing (FDM) were screened, and then the high precision mechanical samples and bioporous scaffolds with good biocompatibility, cell value-added and differentiation were 3D printed. The results show that the addition of PEG improves the toughness of PLA and lowers the melting point of PLA. The addition of HA increases the elastic modulus and cold crystallization temperature of PLA/PEG/HA composites, and HA can also improve the flowability of PLA/PEG/HA composites. SEM and fluorescent labeling results show that the porous scaffold has good biocompatibility. The successful cultivation of bioscaffolds in vitro cells provides potential for further exploration of bioporous scaffolds in animals, biomedicine, and customized applications.

It is a common challenge for the surgeon to detect pathological tissues and determine the resection margin during a minimally invasive surgery. In this study, we present a drop-in sensor probe based on the electrical bioimpedance spectroscopic technology, which can be grasped by a laparoscopic forceps and controlled by the surgeon to inspect suspicious tissue area conveniently. The probe is designed with an optimized electrode and a suitable shape specifically for Minimally Invasive Surgery (MIS). Subsequently, a series of ex vivo experiments are carried out with porcine liver tissue for feasibility validation. During the experiments, impedance measured at frequencies from 1 kHz to 2 MHz are collected on both normal tissues and water soaked tissue. In addition, classifiers based on discriminant analysis are developed. The result of the experiment indicate that the sensor probe can be used to measure the impedance of the tissue easily and the developed tissue classifier achieved accuracy of 80% and 100% respectively.

Objective To explore the effect of Traditional Chinese Medicine (TCM) nursing management for rheumatoid arthritis patients with damp-heat arthralgia spasm syndrome. Methods A total of 398 hospitalized patients with rheumatoid arthritis of damp-heat arthritis syndrome were selected. In addition to routine care, TCM nursing interventions such as Chinese herbal bathing, auricular point pressing therapy and cupping therapy were carried out. The symptoms such as joint swelling and pain, morning stiffness, fatigue and weakness were scored by digital scoring method on the day of hospitalization and the day before discharge. Results T scores of morning stiffness, joint swelling and pain, fatigue and weakness on the day before discharge were significantly lower than those on the day of admission, and the difference was statistically significant (P

Objective To screen the genes associated with fluconazole resistance in Trichosporon asahii to facilitate mechanistic studies of fluconazole resistance and the development of new antifungal agents. Methods Three clinical isolates of fluconazole-sensitive Trichosporon asahii were cultured in the presence of increasing concentrations of fluconazole until the drug concentration reached 128 μg/mL. After further culture for 30 generations in the absence of fluconazole, the MIC value of the induced bacteria was measured. The total RNA was extracted from both the sensitive and resistant strains of the bacteria for construction of the stranded RNA-Seq libraries and transcriptome sequencing to identify the differentially expressed genes (DEGs) in the resistant strains. The genomes of the 3 sensitive and resistant strains were sequenced for comparison of the sequence of the differentially expressed ERG11 gene. Results We successfully obtained stable Trichosporon asahii strains with fluconazole resistance. Transcriptome sequencing data showed that the genes associated with drug transport, ergosterol synthesis and antioxidation were up-regulated in the resistant strains, and among them ERG11 gene, the target gene of fluconazole, was up-regulated by 3.7 folds. Genomic sequencing proved no mutations in the sequence of ERG11 gene in the 3 resistant strains. Conclusion The molecular mechanism for drug resistance in Trichosporon asahii is complex. The upregulation of ergosterol synthesis pathway, especially ERG11 gene, along with other genes associated with efflux transfer and oxidative stress, is one of the main mechanisms for drug resistance in Trichosporon asahii.

Enolases are enzymes in the glycolytic pathway, which catalyse the reversible conversion of D-2-phosphoglycerate into phosphoenol pyruvate in the second half of the pathway. In this research, the effects of α-enolase (ENO1) on steroid reproductive-related hormone receptor expression and on hormone synthesis of primary granulosa cells from goose F1 follicles were studied.

Zn-Al-Mg alloy coating, the most promising protective steel coating of the 21st century, is widely employed in construction, automotive, and other fields, due to its high surface and edge corrosion. In recent years, with the increasing demand for Zn-Al-Mg coating, a series of basic studies on Zn-Al-Mg coating materials has been carried out by foreign scholars, making significant progress and achievements. Simultaneously, the gap in the galvanizing industry between domestic and international has been expanding year by year. In order to gradually reduce gradually this gap with foreign countries, it is necessary to summarize and review the research achievements of foreign researchers. In this paper, the research progress into high corrosion resistant Zn-Al-Mg hot dip coatings was reviewed from the perspective of interfacial reactions in pots, coating structures, corrosion mechanisms of surface and cut edges, as well as corrosion product types of Zn-Al-Mg coatings. According to the range of Al content, laboratory and commercial Zn-Al-Mg coatings are divided into three types: "low-aluminum, " "medium-aluminum, " and "high-aluminum" coatings. There are differences in these coatings, including growth kinetics in the intermetallic compound layers of the different types of coating. In order to control the thickness of the coating, reasonable immersion time and temperature should be controlled. There are also differences in the solidification structures of the three types. Primary Al or Zn crystal, Zn/MgZn2 binary eutectics, and Zn/MgZn2/Al ternary eutectics would form in "low-aluminum" and "medium-aluminum, " while Al-rich dendrites, an intergranular Zn-rich phase, a Mg2Si phase, and a MgZn2 phase would occur with "high-aluminum" coatings. During surface corrosion in "low-aluminum" or "medium-aluminum, " the MgZn2 phase is ionized first, giving rise to a dense corrosion product to inhibit corrosion, such as basic zinc salt (BZS) or layered double hydroxide (LDH). Meanwhile, in the cut edge, a self-healing phenomenon occurs; the proposed explanation in this paper for this is Mg-containing corrosion product flowing or pH changing. However, there are some disputed aspects that need further study. In the hot dipping process, the intermetallic compound thickness should be controlled by the interfacial reaction at the steel/liquid melts through changing the molten bath temperature and holding time. The influence of Mg2Zn11 phase and MgZn2 on the corrosion resistance of Zn-Al-Mg coating is also controversial, so that the microstructure of Zn-Al-Mg coating needs further investigation for corrosion. Furthermore, a kinetic model of the corrosion process should be established to discover the controlling factors in the corrosion reaction, so that the life of the coating can be extended.

BACKGROUND The Xichuan turquoise mining area in Henan Province belongs to the east extension of the turquoise metallogenic belt in the junction of Hubei and Shaanxi Provinces. A lot of turquoise artifacts have been excavated in many early archaeological sites near Xichuan. Therefore, the distribution of the Xichuan turquoises has attracted wide attention of many archaeologists. OBJECTIVES To study mineralogical characteristics of Xichuan turquoise in order to improve the mineralogical study of turquoise in different mining areas in China and provide important data support for tracing the mineral resources of early turquoise artifacts in China. METHODS Technologies including polarizing micro-scope, scanning electron microscope, X-ray diffraction were applied to study the texture and structure of turquoise. Electron probe microanalysis and back scattered electron imaging were applied to study the mineral assemblage of Xichuan turquoise. RESULTS Xichuan turquoise, ring-like, nodular and patchy granular was primarily composed of fine scaly, short columnar and lamellar microcrystalline aggregates. By comparing the microcrystalline structures of different samples, it was noted that the sizes, shapes and distributions could affect the densification and color of Xichuan turquoise. Quartz, chalcedony and iron oxide were common in the Xichuan turquoise aggregate. Svanbergite could also be observed in some samples. CONCLUSIONS By comparing the mineral assemblages of turquoise in other mining areas, it was found that Xichuan turquoise was similar to the turquoise in Hubei Province with quartz and limonite as main mineral impurities, different from the turquoise in Anhui Province with kaolinite as main mineral impurities.

A developed and verified thermodynamic model based on the atom and molecule coexistence theory (AMCT) is employed to predict activities relative to pure liquids in standard state in Mg-Al, Mg-Zn, Al-Zn and Mg-Al-Zn melts through the calculated mass action concentrations of structural units, i.e., Ni. According to AMCT, Ni can be extrapolated and calculated by the chemical equilibrium constant of a structural molecule, i.e., Ki, in the Mg-Al-Zn ternary system and binary subsystems. In this paper, the standard Gibbs free energy function, for reported activities and mixing thermodynamic properties in Mg-Al, Mg-Zn and Al-Zn melts, was regressed and optimized. The results showed that Ki and Ni were deduced by Gibbs free energy function at the studied temperature. The results of calculating thermodynamic properties in the full composition range for liquid Mg-Al-Zn from 880 to 1100 K, as well as Mg-Al from 923 to 1073 K, Mg-Zn from 880 to 973 K and Al-Zn from 1000 to 1073 K, are presented in the paper by coupling with Ni and AMCT. An excellent agreement is noticed between the calculated values of this study and measured thermodynamic data from the references, suggesting that the AMCT can be well applied to describe and predict the activities of the Mg-Al-Zn system and its subsystems.

Inconel 718, a precipitation strengthening nickel-based superalloy, is widely used in aerospace and other high-temperature process industries because of its adequate strength, ductility, and good workability.The increasing demand for alloys with remarkable comprehensive performance necessitates the development of alloys with highly uniform microstructures and highly homogeneous composition;therefore, electroslag remelting (ESR) has attracted much attention in metallurgical circles.However, strong chemical reactions, such as (Al2O3) +[Ti]= (Ti O2) +[Al], usually occur at the electrode-slag interface (ESI) or the droplet-slag interface (DSI) and the metal pool-slag interface (MSI) ;therefore, the concentrations of Al and Ti cannot be maintained within specifications, or the elements cannot be uniformly distributed along the height of the ingots during the ESR process.Consequently, mechanical property deteriorates and the production cost of nickel-based alloy increases.To overcome these issues, in this study, a thermodynamic model for calculating equilibrium content of Al and Ti in a nickel-based alloy during ESR was developed based on the ion and molecule coexistence theory (IMCT) .The chemical composition of slags plays a key role in the physicochemical properties of slags and controls the loss of alloying element during ESR process.Therefore, the respective relations between the activity and activity ratio of individual slag components with chemical composition of Ca O-Si O2-MgO-Fe O-Al2O3-Ti O2-Ca F2slag were studied.Furthermore, effect of the slag composition on the equilibrium content of Al and Ti in Inconel 718 superalloy under various metallurgical temperatures during ESR was investigated.The results indicate that the equilibrium content of Al increases with increasing temperature, however, the equilibrium content of Ti decreases.MgO and Ca F2have little influence on the control of the loss of active alloy elements.

The development of Long-term Care Insurance (LTCI), which matches that people get old before becoming rich lag behind. The mega-urban agglomeration formed in the metropolitan area under the process of urbanization intensifies the demand for insurance driven by long-term care needs. This paper constructs a theoretical model to sort out the micro-action mechanism of LTCI products entering the production field through the consumption of corporate security services, and at the same time entering the consumption field in the form of security services demand from families, individuals and government agencies, it reveals that the differences in the micro-paths of LTCI products entering the enterprise production field and the household consumption field under different regional economic and social conditions. Then, using the GM (1,1) model to analyze the demand for LTCI under the aging population trend of Shanghai, a super large city in China, we found that the situation and characteristics of Shanghai and Japan are similar, and the number of people who need care under the distribution of the elderly population in Shanghai from 2017 to 2027 is much lower than the number of people who need LTCI. Meanwhile, the current long-term nursing service has a large labor gap and high professional skills requirements, but the current salary is low. Besides, the share of elderly care institutions is small, which is not enough to support the current occupancy needs of severely disabled people. The actual development has a greater potential impact on public health expenditures, especially the increase in public health expenditures after the outbreak of the COVID-19 has brought uncertainty. Based on the above findings, we propose to introduce the successful mode of American political and commercial cooperation and the Japanese social security model to alleviate the rapid growth in the demand for LTCI accompanied by the aging trend in the mega-urban clusters formed in the urbanization process.