1. ZnO Tetrapods for Label-Free Optical Biosensing: Physicochemical Characterization and Functionalization Strategies
- Author
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Monica Terracciano, Simas Račkauskas, Andrea Patrizia Falanga, Sara Martino, Giovanna Chianese, Francesca Greco, Gennaro Piccialli, Guido Viscardi, Luca De Stefano, Giorgia Oliviero, Nicola Borbone, and Ilaria Rea
- Subjects
surface characterization ,quantum yield ,label-free detection ,zinc oxide nanostructure ,surface functionalization ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
In this study, we fabricated three different ZnO tetrapodal nanostructures (ZnO-Ts) by a combustion process and studied their physicochemical properties by different techniques to evaluate their potentiality for label-free biosensing purposes. Then, we explored the chemical reactivity of ZnO-Ts by quantifying the available functional hydroxyl groups (–OH) on the transducer surface necessary for biosensor development. The best ZnO-T sample was chemically modified and bioconjugated with biotin as a model bioprobe by a multi-step procedure based on silanization and carbodiimide chemistry. The results demonstrated that the ZnO-Ts could be easily and efficiently biomodified, and sensing experiments based on the streptavidin target detection confirmed these structures’ suitability for biosensing applications.
- Published
- 2023
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