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Insight into the interaction of inhaled corticosteroids with human serum albumin: A spectroscopic-based study
- Source :
- Journal of Pharmaceutical Analysis, Journal of Pharmaceutical Analysis, Vol 8, Iss 1, Pp 37-44 (2018)
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- It is well known that the safety and efficacy profile of an inhaled cortocosteroid (ICS) is influenced by the pharmacokinetic properties and associated pharmacodynamic effects of the drug. Freely circulating, protein unbound, and active ICS can cause systemic adverse effects. Therefore, a detailed investigation of drug-protein interaction could be of great interest to understand the pharmacokinetic behaviour of corticosteroids and for the design of new analogues with effective pharmacological properties. In the present work, the interaction between some corticosteroids and human serum albumin (HSA) has been studied by spectroscopic approaches. UV–Vis spectroscopy confirmed that all the investigated corticosteroids can bind to HSA forming a protein-drug complex. The intrinsic fluorescence of HSA was quenched by all the investigated drugs, which was rationalized in terms of a static quenching mechanism. The thermodynamic parameters determined by the Van’t Hoff analysis of the binding constants (negative ΔH and ΔS values) clearly indicate thathydrogen bonds and van der Waals forces play a major role in the binding process between albumin and betamethasone, flunisolide and prednisolone, while hydrophobic forces may play a major role in stabilizing albumin-triamcinolone complexes.
- Subjects :
- Drug
Stereochemistry
media_common.quotation_subject
Pharmaceutical Science
02 engineering and technology
Pharmacy
010402 general chemistry
01 natural sciences
Analytical Chemistry
Hydrophobic effect
symbols.namesake
Pharmacokinetics
Drug Discovery
Electrochemistry
medicine
Flunisolide
Fluorescence resonance energy transfer (FRET)
Fluorescence spectroscopy
Human serum albumin
Inhaled corticosteroids
Spectroscopy
media_common
Chemistry
lcsh:RM1-950
Albumin
021001 nanoscience & nanotechnology
0104 chemical sciences
lcsh:Therapeutics. Pharmacology
Pharmacodynamics
symbols
Biophysics
Original Article
van der Waals force
0210 nano-technology
medicine.drug
Subjects
Details
- ISSN :
- 20951779
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical Analysis
- Accession number :
- edsair.doi.dedup.....2d14a46f7afb5e8e40947c0e3cad7982