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6 results on '"Gubareva EV"'

1. [Use of immunological and molecular biological methods to diagnose cerebral toxoplasmosis in HIV infection].

Author

Gubareva EV, Goncharov DB, Domonova ÉA, Sil'veĭstrova OIu, Peregudova AB, Tishkevich OA, Ievleva ES, Kobets NV, and Shipulina OIu

Subjects
Adult, Antibodies, Protozoan cerebrospinal fluid, Brain parasitology, Brain virology, Coinfection, DNA, Protozoan cerebrospinal fluid, Disease Progression, Female, HIV Infections blood, HIV Infections cerebrospinal fluid, HIV Infections virology, Humans, Immunoassay, Immunoglobulin A blood, Immunoglobulin A cerebrospinal fluid, Immunoglobulin G blood, Immunoglobulin G cerebrospinal fluid, Immunoglobulin M blood, Immunoglobulin M cerebrospinal fluid, Magnetic Resonance Imaging, Male, Middle Aged, Polymerase Chain Reaction, Toxoplasma immunology, Toxoplasmosis, Cerebral blood, Toxoplasmosis, Cerebral cerebrospinal fluid, Toxoplasmosis, Cerebral parasitology, Antibodies, Protozoan blood, Brain pathology, DNA, Protozoan blood, HIV, HIV Infections pathology, Toxoplasma isolation & purification, Toxoplasmosis, Cerebral diagnosis
Abstract

Cerebral toxoplasmosis is one of the leading causes of neurologic diseases with high mortality rates in patients with HIV infection. Invasion was difficult to diagnose for a number of objective reasons. The objective of the investigation was to determine the clinical sensitivity of different laboratory techniques as both a single study and their various combinations to verify the diagnosis of cerebral toxoplasmosis in HIV-infected patients. Blood and cerebrospinal fluid were tested in 51 patients with Stage 4B HIV infection (AIDS) with the verified diagnosis of cerebral toxoplasmosis. Separate determination of specific antibodies of IgG, IgM, IgA and toxoplasma DNA in the blood and cerebrospinal fluid was shown to have an insufficient clinical sensitivity (37.3-68.6%). The benefits of various combinations of immunological and molecular biological assays enhancing the diagnostic efficiency up to 76.5-96.1% are demonstrated.

Published
2013

2. [Cell mechanisms that control Toxoplasma gondii dissemination in parenteral and oral infection].

Author

Kobets NV, Domonova ÉA, Goncharov DB, Mezentseva MV, Sil'veĭstrova Oi, Gubareva EV, Shapoval IM, and Ievleva ES

Subjects
Administration, Oral, Animals, Antigens, CD genetics, Antigens, CD immunology, B-Lymphocytes immunology, B-Lymphocytes pathology, Cell Movement, Cytokines blood, Cytokines immunology, Dendritic Cells immunology, Dendritic Cells pathology, Flow Cytometry, Genetic Predisposition to Disease, Immunophenotyping, Injections, Intraperitoneal, Macrophages immunology, Macrophages pathology, Mice, Mice, Inbred BALB C, Mice, Transgenic, Neutrophils immunology, Neutrophils pathology, Survival Rate, T-Lymphocytes immunology, T-Lymphocytes pathology, Toxoplasma pathogenicity, Toxoplasmosis, Animal genetics, Toxoplasmosis, Animal mortality, Toxoplasmosis, Animal pathology, DNA, Protozoan blood, Host-Parasite Interactions, Toxoplasma physiology, Toxoplasmosis, Animal immunology
Published
2012

3. [Toxoplasmosis in HIV infection: invasion reactivation criteria].

Author

Goncharov DB, Gubareva EV, Kobets NV, Domonova EA, and Ievleva ES

Subjects
Antibodies, Protozoan immunology, Biomarkers analysis, CD4 Lymphocyte Count, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes pathology, Coinfection, HIV physiology, HIV Infections blood, HIV Infections immunology, Humans, Immunoglobulin G blood, Immunoglobulin G immunology, Magnetic Resonance Imaging, Recurrence, Toxoplasma physiology, Toxoplasmosis, Cerebral blood, Toxoplasmosis, Cerebral immunology, Antibodies, Protozoan blood, HIV Infections virology, RNA, Protozoan blood, Toxoplasma pathogenicity, Toxoplasmosis, Cerebral diagnosis, Toxoplasmosis, Cerebral parasitology
Abstract

Contemporary representation of toxoplasmosis reactivation criteria in HIV infection is generalized. Significance of the issue is justified: toxoplasmosis is a leading neurological pathology in AIDS with a high lethality percentage due to complexity of clinical confirmation and difficulties of laboratory confirmation of the start of reactivation. Clinical, instrumental, immunologic, molecular genetic invasion reactivation criteria are discussed in the article and analysis of their effectiveness is performed; their most feasible combinations are justified. Further system analysis of the cerebral toxoplasmosis reactivation criteria specified in the article in combination with search of new pathogen dissemination markers will allow to obtain important information that has both fundamental interest and important practical significance.

Published
2012

4. [Features of epidemiology and diagnostics of toxoplasmosis during HIV-infection].

Author

Gubareva EV, Goncharov DB, Peregudova AB, Parkhomenko IuG, Tishkevich IM, Tishkevich OA, and Kobets NV

Subjects
AIDS-Related Opportunistic Infections complications, Adult, Aged, Antibodies, Protozoan blood, Antibodies, Protozoan cerebrospinal fluid, Antigens, Protozoan analysis, Antigens, Protozoan immunology, Blotting, Western methods, Epitopes analysis, Epitopes immunology, Fluorescent Antibody Technique, Indirect, Humans, Immunoglobulin G analysis, Immunoglobulin G blood, Immunoglobulin G cerebrospinal fluid, Middle Aged, Russia epidemiology, Toxoplasma isolation & purification, Toxoplasmosis complications, Toxoplasmosis, Cerebral diagnosis, AIDS-Related Opportunistic Infections diagnosis, AIDS-Related Opportunistic Infections epidemiology, Antibodies, Protozoan analysis, HIV Infections complications, Toxoplasmosis diagnosis, Toxoplasmosis epidemiology
Abstract

Aim: Comparative assessment of effectiveness of serologic methods for toxoplasmosis diagnostics in patients with HIV-infection., Materials and Methods: Sera and CSF samples from 166 patients with AIDS stage IIIB were tested for antibodies to Toxoplasma gondii by indirect immunofluorescence, ELISA and immunoblotting. Results of serological tests were compared with clinical, pathological data as well as with results of MRI and PCR., Results: Clinical value of IgG detection in blood and CSF by ELISA was shown--high level of antibodies marked reactivation of the invasion. IgG antibodies in CSF were detected only if high levels of IgG were present in the blood. Detection of antigenic determinants with molecular mass 18 - 20 and 65 - 70 kDa in immunoblotting was proposed as a criterion of cerebral toxoplasmosis reactivation., Conclusion: Complex laboratory serologic tests along with data obtained by MRI, PCR and microscopy of T. gondii cysts enhances the effectiveness of toxoplasmosis diagnostics. Knowledge of toxoplasmosis reactivation criteria in patients with AIDS will allow to develop the optimal protocol of toxoplasmosis diagnostics and substantiate measures for its prevention.

Published
2010

5. [Immune response in toxoplasmosis after mucosal immunization with Toxoplasma gondii antigens].

Author

Kobets NV, Zaĭtseva LG, Goncharov DB, Gubareva EV, Mezentseva MV, Kireeva IV, Surgucheva IM, and Samoĭlenko II

Subjects
Adjuvants, Immunologic administration & dosage, Administration, Oral, Animals, Antibodies, Protozoan blood, Antibody Specificity, Antigens, Protozoan administration & dosage, Cells, Cultured, Cytotoxins analysis, Cytotoxins genetics, Hyaluronic Acid administration & dosage, Macrophages immunology, Mice, Mice, Inbred DBA, Mouth Mucosa, Protozoan Vaccines administration & dosage, RNA, Messenger analysis, Reverse Transcriptase Polymerase Chain Reaction, Spleen immunology, Toxoplasmosis blood, Toxoplasmosis immunology, Antigens, Protozoan immunology, Protozoan Vaccines immunology, Toxoplasma immunology, Toxoplasmosis prevention & control, Vaccination
Published
2010

6. Evaluation of the resistance of blood plasma to oxidative stress by oxidizability of proteins and lipids during metal-catalyzed oxidation.

Author

Bekman EM, Baranova OA, Gubareva EV, Shulenina LV, Moskvina SN, Danilogorskaya YA, and Azizova OA

Subjects
Buffers, Catalysis, Chelating Agents pharmacology, Copper metabolism, Copper pharmacology, Cross-Linking Reagents metabolism, Deferoxamine pharmacology, Dose-Response Relationship, Drug, Evaluation Studies as Topic, Fibrinogen metabolism, Hydrogen-Ion Concentration, Iron metabolism, Iron pharmacology, Metals metabolism, Oxidation-Reduction, Protein Carbonylation drug effects, Sodium Chloride chemistry, Spectrometry, Fluorescence, Time Factors, Lipid Metabolism, Malondialdehyde blood, Metals pharmacology, Oxidative Stress, Plasma metabolism, Proteins metabolism
Abstract

A new approach for the evaluation of oxidizability of proteins and lipids in the same sample of blood plasma was proposed. We tested a method for evaluation of metal-catalyzed oxidation of fibrinogen by the formation of bityrosine cross-links during oxidation detected by the increase in fluorescence at 415 nm. A correlation was revealed between parameters of oxidizability estimated by this marker and carbonyl derivatives (dinitrophenylhydrazine method). Oxidizability of total proteins from whole plasma was compared with oxidizability of plasma lipids (marker malonic dialdehyde). Study of these parameters in patients with coronary heart disease showed that the proposed experimental approach allows us to divide the sample into several subgroups differing in the resistance to oxidative stress. These data can be used for diagnostic and prognostic purposes.

Published
2006
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