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[Features of epidemiology and diagnostics of toxoplasmosis during HIV-infection].
- Source :
-
Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2010 Mar-Apr (2), pp. 28-32. - Publication Year :
- 2010
-
Abstract
- Aim: Comparative assessment of effectiveness of serologic methods for toxoplasmosis diagnostics in patients with HIV-infection.<br />Materials and Methods: Sera and CSF samples from 166 patients with AIDS stage IIIB were tested for antibodies to Toxoplasma gondii by indirect immunofluorescence, ELISA and immunoblotting. Results of serological tests were compared with clinical, pathological data as well as with results of MRI and PCR.<br />Results: Clinical value of IgG detection in blood and CSF by ELISA was shown--high level of antibodies marked reactivation of the invasion. IgG antibodies in CSF were detected only if high levels of IgG were present in the blood. Detection of antigenic determinants with molecular mass 18 - 20 and 65 - 70 kDa in immunoblotting was proposed as a criterion of cerebral toxoplasmosis reactivation.<br />Conclusion: Complex laboratory serologic tests along with data obtained by MRI, PCR and microscopy of T. gondii cysts enhances the effectiveness of toxoplasmosis diagnostics. Knowledge of toxoplasmosis reactivation criteria in patients with AIDS will allow to develop the optimal protocol of toxoplasmosis diagnostics and substantiate measures for its prevention.
- Subjects :
- AIDS-Related Opportunistic Infections complications
Adult
Aged
Antibodies, Protozoan blood
Antibodies, Protozoan cerebrospinal fluid
Antigens, Protozoan analysis
Antigens, Protozoan immunology
Blotting, Western methods
Epitopes analysis
Epitopes immunology
Fluorescent Antibody Technique, Indirect
Humans
Immunoglobulin G analysis
Immunoglobulin G blood
Immunoglobulin G cerebrospinal fluid
Middle Aged
Russia epidemiology
Toxoplasma isolation & purification
Toxoplasmosis complications
Toxoplasmosis, Cerebral diagnosis
AIDS-Related Opportunistic Infections diagnosis
AIDS-Related Opportunistic Infections epidemiology
Antibodies, Protozoan analysis
HIV Infections complications
Toxoplasmosis diagnosis
Toxoplasmosis epidemiology
Subjects
Details
- Language :
- Russian
- ISSN :
- 0372-9311
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Zhurnal mikrobiologii, epidemiologii i immunobiologii
- Publication Type :
- Academic Journal
- Accession number :
- 20464997