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Centrioles are submicron-scale, barrel-shaped organelles typically found in pairs, and play important roles in ciliogenesis and bipolar spindle assembly. In general, successful execution of centriole-dependent processes is highly reliant on the ability of the cell to stringently control centriole number. This in turn is mainly achieved through the precise duplication of centrioles during each S phase. Aberrations in centriole duplication disrupt spindle assembly and cilia-based signaling and have been linked to cancer, primary microcephaly and a variety of growth disorders. Studies aimed at understanding how centriole duplication is controlled have mainly focused on the post-translational regulation of two key components of this pathway: the master regulatory kinase ZYG-1/Plk4 and the scaffold component SAS-6. In contrast, how transcriptional control mechanisms might contribute to this process have not been well explored. Here we show that the chromatin remodeling protein CHD-1 contributes to the regulation of centriole duplication in the C. elegans embryo. Specifically, we find that loss of CHD-1 or inactivation of its ATPase activity can restore embryonic viability and centriole duplication to a strain expressing insufficient ZYG-1 activity. Interestingly, loss of CHD-1 is associated with increases in the levels of two ZYG-1-binding partners: SPD-2, the centriole receptor for ZYG-1 and SAS-6. Finally, we explore transcriptional regulatory networks governing centriole duplication and find that CHD-1 and a second transcription factor, EFL-1/DPL-1 cooperate to down regulate expression of CDK-2, which in turn promotes SAS-6 protein levels. Disruption of this regulatory network results in the overexpression of SAS-6 and the production of extra centrioles., Competing Interests: The authors have declared that no competing interests exist.

Helminth infections within tissues tend to be subjected to a host response that can include encapsulation and melanization to isolate the parasite. The effectiveness of this response depends on the host species. During a survey of parasites of the native fish, Galaxias maculatus , we found conspicuous, strongly melanized exterior cysts located in the caudal peduncle of the fish. Dissection of these cysts exposed larval nematodes whose morphometrical features allowed their identification as Eustrongylides sp. Species of this genus are distributed worldwide. Galaxias maculatus was previously reported as second intermediate host to Eustrongylides sp. larvae, and the aquatic bird Podiceps major was cited as definitive host of Eustrongylides tubifex in Andean Patagonian lakes. The site of infection in the fish, and the host's response are unique among infections by larvae of Eustrongylides sp. in fishes, so the objective of this study was to describe the histological injury caused by larvae of the genus Eustrongylides parasitizing G. maculatus . Samples of fish were taken near the shore of Gutiérrez lake, in northwestern Patagonia. Some larval morphometric features were described to confirm the identity of larvae to generic level. Histopathological analysis (tissues sectioned at 5-7 μm, stained with Hematoxylin and Eosin, and Masson's Trichrome) of the caudal peduncle revealed almost complete disappearance of the epaxial musculature due to compressive atrophy and a chronic inflammatory response, associated mainly with a dense fibrotic capsule and an intense melanic deposit. This is the first description of the histopathology of an external cyst caused by Eustrongylides sp. larvae in fishes.

The pejerrey Odontesthes bonariensis is the most important freshwater fish of Argentina central region. The present study was conducted to describe the seasonal variation of parasitism in O. bonariensis from La Viña reservoir (31 degrees 17'S, 65 degrees 01'W), located in the province of Córdoba, Argentina. Between years 1999 and 2002, 573 fishes were caught, and their parasite infection and relative weight (Wr) were assesed. Three parasites were found, Lernaea cyprinacea (Crustacea), Contracaecum spp. larvae (Nematoda) and Cangatiella macdonaghi (Cestoda). Overall, Contracaecum spp. prevalence and intensity were very low. Lernaea cyprinacea and Cangatiella macdonaghi prevalences increased during summer and spring (P < 0.01), respectively. Lernaea cyprinacea infection showed also seasonal differences in intensity (P < 0.05), as well as C. macdonaghi regarding parasites density (P < 0.01). Cangatiella macdonaghi average parasites density was 32.0 (+/-22.5) cestodes per cm(3)of digestive content. No association between parasitism and fish relative weight was found. Besides the seasonality variations observed, the parasite burden found did not compromise fish body condition.