1. In vitro faecal fermentation metabolites of 2′-fucosyllactose protect against intestinal epithelial injury: Infant enterotype effects.
- Author
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Gu, Zhipeng, Xu, Shiqi, Zheng, Yuxing, Lane, Jonathan A., Dhital, Sushil, Huang, Qiang, Hu, Ruibiao, Liu, Feitong, and Zhang, Bin
- Subjects
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INTESTINAL injuries , *BIFIDOBACTERIUM longum , *FERMENTATION , *BUTYRATES , *INFANTS , *TIGHT junctions - Abstract
In this study, we conducted in vitro fermentation with infant faecal inocula dominated by Bifidobacterium longum , Bifidobacterium breve , and Bacteroides enterotypes, and assessed the efficacy of the metabolome associated with 2′-fucosyllactose (2′-FL) fermentation on lipopolysaccharide-induced epithelial cell barrier damage on Caco-2 cells. The results revealed that propionate and butyrate were more prominent in the Bacteroides -dominated group, whereas the Bifidobacterium -dominated group was associated with an increased molar fraction of lactate (∼16 m m). Additionally, our study demonstrated that 2′-FL faecal metabolites (FMs) decreased the production of inflammatory cytokines, and promoted tight junction gene expression by inhibiting the TLR4/NF-κB/MLCK signalling pathway and activating the Nrf2/HO-1 pathway. The 2′-FL FMs from Bacteroides -dominated enterotypes had a unique advantage in regulating the TLR4/NF-κB/MLCK signalling pathway, which can be attributed to the effects of propionate and butyrate. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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