Biocompatibility of genipin-fixed porcine aorta as a possible esophageal prosthesis

Abstract: In this study, porcine aortic tissues were fixed with genipin to construct an esophageal prosthesis. In vitro characterization was performed and in vivo biocompatibility experiments were undertaken to evaluate the biological properties of genipin-fixed porcine aortic tissues. The porcine aortic tissues fixed with genipin at pH 4.0, 0.625g/100ml initial fixative concentrations for 7days showed more favorable characteristics. It was characterized by low antigenicity, good epithelial cellular compatibility, favorable biomechanical properties for an esophageal prosthesis, high resistance against enzymatic degradation and be readily available. The porcine aortic tissues fixed with genipin also showed good biocompatibility in vivo. The present study demonstrated that the genipin-fixed porcine aortic tissues could be a promising material for the construction of an esophageal prosthesis or fabricating scaffold for a tissue engineered esophagus. [Copyright &y& Elsevier]