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Our exploratory study analyzes the civic-mindedness of university graduates in an engaged university with emphasis on Central and Eastern Europe, particularly Slovakia. The research sample consisted of 452 graduates of the second level of university studies. To map and analyze civic-mindedness, we used the Civic-Minded Graduate Questionnaire (CMG; Steinberg et al., 2011). At the selected university, graduates scored the highest in skills and dispositions and the lowest in behavioral intentions. Furthermore, we found that those graduates who volunteered during their university studies had statistically significant greater development in the areas knowledge, skills, dispositions, and behavioral intentions, as well as in CMG scale overall, than those who did not participate in volunteering. Our study showed that the CMG concept is usable in countries with different contexts of the development of the university environment and the idea of citizenship and can help map the level of civic-mindedness among university graduates.

Abstract Multiple myeloma (MM) is the second most prevalent hematological malignancy, characterized by infiltration of the bone marrow by malignant plasma cells. Extramedullary disease (EMD) represents a more aggressive condition involving the migration of a subclone of plasma cells to paraskeletal or extraskeletal sites. Liquid biopsies could improve and speed diagnosis, as they can better capture the disease heterogeneity while lowering patients’ discomfort due to minimal invasiveness. Recent studies have confirmed alterations in the proteome across various malignancies, suggesting specific changes in protein classes. In this study, we show that MALDI-TOF mass spectrometry fingerprinting of peripheral blood can differentiate between MM and primary EMD patients. We constructed a predictive model using a supervised learning method, partial least squares-discriminant analysis (PLS-DA) and evaluated its generalization performance on a test dataset. The outcome of this analysis is a method that predicts specifically primary EMD with high sensitivity (86.4%), accuracy (78.4%), and specificity (72.4%). Given the simplicity of this approach and its minimally invasive character, this method provides rapid identification of primary EMD and could prove helpful in clinical practice.

Efficient reading is essential for societal participation, so reading proficiency is a central educational goal. Here, we use an individualized diagnostics and training framework to investigate processes in visual word recognition and evaluate its usefulness for detecting training responders. We (i) motivated a training procedure based on the Lexical Categorization Model (LCM) to introduce the framework. The LCM describes pre-lexical orthographic processing implemented in the left-ventral occipital cortex and is vital to reading. German language learners trained their lexical categorization abilities while we monitored reading speed change. In three studies, most language learners increased their reading skills. Next, we (ii) estimated, for each word, the LCM-based features and assessed each reader's lexical categorization capabilities. Finally, we (iii) explored machine learning procedures to find the optimal feature selection and regression model to predict the benefit of the lexical categorization training for each individual. The best-performing pipeline increased reading speed from 23% in the unselected group to 43% in the machine-selected group. This selection process strongly depended on parameters associated with the LCM. Thus, training in lexical categorization can increase reading skills, and accurate computational descriptions of brain functions that allow the motivation of a training procedure combined with machine learning can be powerful for individualized reading training procedures.

Abstract The generation and optimization of simulation data for electrical machines remain challenging, largely due to the complexities of magneto-static finite element analysis. Traditional methodologies are not only resource-intensive, but also time-consuming. Deep learning models can be used to shortcut these calculations. However, challenges arise when considering the unique parameter sets specific to each machine topology. Building on two recent studies (Parekh et al. in IEEE Trans. Magn. 58(9):1–4, 2022; Parekh et al., Deep learning based meta-modeling for multi-objective technology optimization of electrical machines, 2023, arXiv: 2306.09087 ), that utilized a variational autoencoder to cohesively map diverse topologies into a singular latent space for subsequent optimization, this paper proposes a refined architecture and optimization workflow. Our modifications aim to streamline and enhance the robustness of both the training and optimization processes, and compare the results with the variational autoencoder architecture proposed recently.

Silica refractories are promising materials for high-temperature thermal energy storage (HT-TES), because they exhibit excellent thermal cycling properties, unless cooled below a critical temperature (usually assumed to be 600 °C). When cooling down to room temperature severe damage can occur, which can be conveniently monitored via the impulse excitation technique (IET). This damage is most severe when the cycling maximum temperature is low. The question is whether this damage can be healed again. In this short contribution we show that severe damage in silica refractories, caused by heating from room temperature to 300 °C and back again to room temperature, can indeed be healed by thermal cycling to 1300 °C. The healed material is actually better than the pristine material.

This article deals with cultural sustainability, authenticity and one of its basic pillars: the preservation of diversity. It looks at constructions around the world and how they have adapted to local geophysical circumstances, both in the construction methods and materials used. It analyses how diversity has been endangered by globalisation in the territory of Slovakia and elsewhere. Subsequently, the article focuses on monument care and preservation in Slovakia and other countries, comparing the legal instruments used in different countries. The article proposes a system for the categorisation of objects built before 1947 in Slovakia. This is key to better understanding the topic of protection of heritage buildings for professionals and government agencies dealing with monument protection. The proposed categorisation could help significantly in systematising the protection of tangible cultural heritage and building culture in Slovakia.

AimThe aim of this study was to develop a vancomycin population pharmacokinetic model in adult obese patients and propose covariate-based dosing individualization in order to maximize the achievement of the newly recommended PK/PD target, according to a revised consensus guideline from 2020.MethodsTherapeutic drug monitoring data from initial vancomycin therapy (first 3 days of treatment) in adult obese (BMI ≥ 30 kg/m2) patients from 2013 to 2022 were analyzed using a non-linear mixed-effects modeling method, and Monte Carlo simulations were then used to find the optimal dosage maximizing the PK/PD target attainment.ResultsA total of 147 vancomycin serum levels obtained from 138 patients were included in the analysis. Based on the covariate model diagnosis among all tested variables, no reliable predictor of vancomycin volume of distribution (Vd) was identified, while clearance (CL) was positively correlated with eGFR and lean body mass. Creatinine-based eGFR predicted vancomycin CL better than cystatin C-based eGFR. The median (interquartile range) value from conditional modes of individual estimates of Vd, CL, and elimination half-life in our population was 74.0 (70.5–75.4) L, 6.65 (4.95–8.42) L/h, and 7.7 (6.0–10.0) h, respectively.ConclusionWe proposed dosing individualization based on the covariate found in order to maximize the achievement of the newly recommended PK/PD target of the AUC/MIC ratio of 400–600. Clinical pharmacy/pharmacology interventions may lead to an improvement in vancomycin dosing with a reflection in PK/PD target attainment.

Targeted therapy with protein kinase inhibitors (PKIs) represents one of the important treatment options for non-small cell lung cancer (NSCLC). It has contributed to improve patients’ survival and quality of life significantly. These anticancer drugs are administrated orally in flat-fixed doses despite the well-known large interpatient pharmacokinetic variability and the possible need for dose individualization. To optimize and individualize dosing of PKIs, and thereby increasing the effectiveness and safety of the treatment, therapeutic drug monitoring (TDM) is the most frequently mentioned method. Unlike other areas of medicine, TDM has been rather exceptional in oncological practise since there is a little evidence or no data for concentration-effect relationships of PKIs. Therefore, the aim of this review is to summarize the pharmacokinetic characteristics of PKIs and provide the evidence supporting the use of TDM for personalised treatment of patients with NSCLC.

This study at a university in Slovakia asked two questions: (1) Does completion of a service-learning course lead to changes in students' social and personal responsibility and their attitudes toward community service? (2) Are personal and social responsibility and attitudes toward community service correlated? Service-learning and the third mission of the university are almost unknown in Slovak higher education, and likewise no relevant test instruments adapted to Slovak conditions currently exist. Consequently, we adopted a scale from abroad--Conrad and Hedin's (1981) Social and Personal Responsibility Scale (SPRS). We also used qualitative research methods. Compared to a control group, the service-learning students had statistically significant higher posttest scores in the Social Welfare, Duty, Performance of Responsible Acts, and Global Responsibility subscales. The research results revealed a need for new measuring tools that are sensitive to the specific context of service-learning implementation in Slovakia.

An analysis of the external pressure coefficient on the surface of a ruin in different flow directions is presented. The ruin has almost cube-like proportions with an open roof plane and a destroyed corner. Flow simulations were performed using 3D Time Steady RANS and compared with experimental results from the boundary layer wind tunnel at the Slovak University of Technology in Bratislava. The optimal turbulence model and internal mesh settings were selected based on their statistical evaluation. For an evaluation of the critical directions of the wind flow around the ruin, the values of the external wind pressure coefficient were obtained from the selected calculation model and settings.

Aim of the study: The aim of this paper is to analyze and assess the land cover change in three transects in Slovakia by applying the coefficient of ecological stability and the coefficient of anthropogenic impact in four different time horizons (1950, 1990, 2000, and 2020). Based on the specific natural and socio-economic factors influencing landscape formation and change, we chose three transects from the territory of Slovakia as study areas. The study areas are located in western Slovakia (transect called Záhorie: Rudava – Buková), central Slovakia (transect called Turiec: Martin – Moškovec), and eastern Slovakia (transect called Poloniny: Stakčín – Ruské). Material and methods: Several methodological tools have been created to express the level of ecological stability of a given territory. In our study, we used the coefficient of ecological stability (CES). The intensity of changes in the landscape as a result of human activity was also described through the coefficient of anthropogenic influence (CAI). Results and conclusions: The results of CES and CAI indicate a similar situation in case of the Záhorie: Rudava – Buková transect and the Poloniny: Stakčín – Ruské transect. For the Poloniny: Stakčín – Ruské transect, the CES values are significantly higher than 1.21, which is a landscape with high ecological stability, and the CAI values are close to 0, which also represents a landscape with a minimal intensity of changes due to human activity. In the case of the Záhorie: Rudava – Buková transect it is similar, but the CES values do not reach such high values as in the case of the Poloniny: Stakčín – Ruské transect, which is mainly influenced by the fact that the Poloniny: Stakčín – Ruské transect passes through a protected area of the Poloniny National Park. In the case of the Turiec: Martin – Moškovec transect, there is no correspondence between the CAI and CES values, as the CAI values are less than 1 (the highest value is 0.46), which represents a landscape with a minimal intensity of changes due to human activity. However, the results of CES are values lower than 0.8, which represents a landscape with low ecological stability.

The main goal of the study, based on historical-geographical research, was to reconstruct the history of an anthropogenically created cultural landscape on the southern edge of the Muránska Planina National Park in the Western Carpathians (Slovakia) as a potential European ground squirrel habitat. Humans began to change the original forest landscape in the second half of the 13th century in connection with the construction of Muráň castle, which became the economic center of the study area. The first written mention of the existence of Muráň castle (castro Muran) dates to 1271. The original deciduous forests were gradually removed and transformed into agricultural land. At the turn of the 14th and 15th centuries, we can almost certainly assume the existence of an agricultural landscape in the territory called Biele Vody (part of the study area) on the right side of the Muránsky Potok valley in such spatial dimensions as it is at present. The landscape created in this way provided suitable ecological conditions for the successful survival of the European ground squirrel (Spermophilus citellus). The analysis and reconstruction of the origins and development of the agricultural landscape were carried out based on detailed archival and terrain research. Map outputs are also the result of the reconstructions. Whether the European ground squirrel was already present in the locality before its conservation translocation (773 individuals were released at the site in 2000–2007) is discussed in detail in this paper. The current ground squirrel colony is dependent on feeding, mainly sunflowers (since 2011), on active management and maintenance of the landscape provided by a herd of donkeys (March–December) and sheep (May–July). The ground squirrel locality Biele Vody is currently a center of ecotourism and ecological education.

The study presents fundamental theoretical and methodological information on individual sacral tourism as a suitable type of tourism during the COVID-19 pandemic. Individual (virtual) tourism on the example of the Koscelisko locality in Radoľa municipality (northwestern part of Slovakia) in the area of the extinct medieval sacral landscape, thanks to modern technologies, represents a relatively modern segment of the alternative - modern types of tourism. In the first step, a bibliometric method combined with content analysis of literary and archival sources was used to process the study. It was followed by a comprehensive method of historical-geographical research and field research, which generate a database of information to create a 3D model of the defunct church Koscelisko. The outputs processed in this way are available online to potential tourists via smartphones on the platform called Multimedia Guide to Geotourism (https://www.montanistika.eu).

Autoimmune adverse effects of immune checkpoint inhibitors (ICIs) are rare but may be life-threatening. We performed a retrospective analysis of the immune-related adverse effects (irAEs) of ICIs in the Masaryk Memorial Cancer Institute (MMCI) from 2011 to 2021. The ICIs were administered to 648 patients. Severe irAEs were pronounced in 83 patients (12.8 %). Further, we report a case of severe irAE after treatment with ipilimumab and nivolumab. Prednisone, mesalazine, octreotide, infliximab, vedolizumab, and mycophenolate mofetil were used to manage G4 colitis, with partial response. Early diagnosis and appropriate management of irAE are essential in the perspective of further oncological treatment.

The process of copper cementation has already been known since the period of antiquity in Europe. Nevertheless, the first historically relevant reports come from the 14th century from the mining town of Smolník in Upper Hungary (present-day Slovakia), which makes this site the oldest place of the commercial production of copper using cementation in Europe. It is one of the oldest known sites in the world after China, where this process has been used since the 11th century. The cementation copper from Smolník was considered to be a high-quality copper in the period between the 14th and 19th century and was an important export product of Hungary. The study processes the history of cementation and discusses the production process of the artificial cementation water, as well as its subsequent mining and sedimentation. A detailed description of the technological progress of cementation from the earliest times up to the first half of the 19th century is given. The study is based upon the historical works of medieval alchemists and the first miners and naturalists, which were published as early scientific books in Europe from the 16th to the 18th century. These findings are complemented by original archival research.

This work aims to present a customer satisfaction model using selected examples from the spa industry. Specifically, it is a presentation of the satisfaction of spa customers and visitors to the Slovak spa of Rajecké Teplice, in comparison with Piešťany and Brusno. Customer satisfaction is a multiple phenomenon which is evaluated mainly by qualitative methods. Our motivation is to draw attention to the difficulty and subjectivity of qualitative research concerning such satisfaction, especially at in-service facilities in selected Slovak spas. Customers ranked them and created a ‘top 12’, according to their importance in the subjective perception of spa environments, services, health services and attractions in terms of satisfaction. In addition, the result is a comparison of the individual satisfaction of spa customers in heterogeneous spa towns. Piešťany is the most important Slovak spa center, Trenčianske Teplice is a medium-sized spa town, and Brusno a small stagnant spa.

Niniejsza praca ma na celu zaprezentowanie modelu satysfakcji klienta na przykładzie wybranych sytuacji z branży uzdrowiskowej. Jest to w szczególności omówienie zagadnienia satysfakcji gości słowackich uzdrowisk w miejscowości Rajecké Teplice w porównaniu do zadowolenia z wizyt w kurortach w Pieszczanach i Brusnie. Satysfakcja klienta jest zagadnieniem wieloaspektowym, które można poddać ocenie głównie przy użyciu metod jakościowych. W artykule zamieszczono oryginalną ankietę satysfakcji klienta. Motywacją autorów było zwrócenie uwagi na subiektywną naturę badań jakościowych dotyczących satysfakcji klienta – szczególnie w obszarze obiektów usługowych, w tym wypadku wybranych słowackich uzdrowisk – oraz na związane z nimi trudności. Wynik badań stanowi jednocześnie porównanie granic indywidualnej satysfakcji klienta w heterogenicznych miejscowościach uzdrowiskowych. Pieszczany są bowiem najważniejszym ośrodkiem uzdrowiskowym na Słowacji, Rajecké Teplice – średniej wielkości miastem uzdrowiskowym, Brusno zaś – niewielkim, nieco gnuśnym uzdrowiskiem. Klienci mieli za zadanie ocenić je oraz stworzyć listę „top 12” – uszeregować spa na podstawie subiektywnego odczucia satysfakcji w odniesieniu do ich otoczenia, oferowanych usług, opieki zdrowotnej i atrakcji.

The cotton plant suffers from many pests and due to its economic importance, the use of efficient but ecologically friendly pesticides is highly desirable. The insecticide Sumi-Alpha with active compound Esfenvalerate is widely used for cotton treatment in Uzbekistan. This insecticide is considered as moderately hazardous for different organisms of ecosystems, while more collected data and relevant research is a necessity for judgement of their safety and/or potential risk. Therefore, a comparative analysis of cotton leaves was performed to study the protein profile changes upon treatment with this insecticide. Our data showed elevated accumulation of fructose bisphosphate aldolase and degradation of RuBisCo in the treated tissue. Besides, a protein belonging to P-loop containing nucleoside triphosphate hydrolases superfamily exerted lower accumulation. Our results indicate that the applied pyrethroid pesticide possibly can affect photosynthesis performance and cause accumulation of simple saccharides which in turn might contribute to enhanced colonisation of sucking insects. Further molecular research on Sumi-Alpha effects on cotton is necessary for its safer use in agricultural practice.

This study analysed the history of anthropogenically created alpine pastures from the 15th century to the present, as landscape resources for the chamois reintroduction in the second half of the 20th century in the Western Carpathians mountain arc (Slovakia), using the example of the Ďumbier Tatras (the second highest mountain range of the arc). Analysis and reconstructions were carried out on the basis of detailed archival and field research, which showed that grazing herds in the mountain peaks from the Middle Ages to the 20th century created anthropogenically suitable and sufficiently extensive grassy habitats for the chamois reintroduction and the survival of its population. The native chamois population became extinct in the Ďumbier Tatras at the end of the last ice age (about 10,000 years ago). However, anthropogenic deforestation has once again created suitable conditions for its distribution. In the 20th century, a new factor emerged, namely nature conservation and the proclamation of a national park, which meant the end of grazing in the alpine environment and the onset of succession. In the second half of the 20th century, modern tourism became another negative factor for the relocated Alpine chamois population, from the High Tatras back to its quasi-original environment. Tourism development was related to the construction of extensive infrastructure and superstructure in the chamois habitats in the Chopok and Ďumbier massifs. At present, therefore, the preservation of these ‘anthropogenic’ habitats requires active conservation and landscape management.

Extramedullary multiple myeloma (EMD) is an aggressive disease; malignant plasma cells lose their dependence in the bone marrow microenvironment and migrate into tissues. EMD is a negative prognostic factor of survival. Using flow cytometry and next-generation sequencing, we aimed to identify antigens and microRNAs (miRNAs) involved in EMD pathogenesis. Flow cytometry analysis revealed significant differences in the level of clonal plasma cells between MM and EMD patients, while the expression of CD markers was comparable between these two groups. Further, miR-26a-5p and miR-30e-5p were found to be significantly down-regulated in EMD compared to MM. Based on the expression of miR-26a-5p, we were able to distinguish these two groups of patients with high sensitivity and specificity. In addition, the involvement of deregulated miRNAs in cell cycle regulation, ubiquitin-mediated proteolysis and signaling pathways associated with infections or neurological disorders was observed using GO and KEGG pathways enrichment analysis. Subsequently, a correlation between the expression of analyzed miRNAs and the levels of CD molecules was observed. Finally, clinicopathological characteristics as well as CD antigens associated with the prognosis of MM and EMD patients were identified. Altogether, we identified several molecules possibly involved in the transformation of MM into EMD.

The majority of timber bridges for ordinary road traffic are often made as timber-concrete composite structures. Concrete slab is something like as umbrella for timber. The reason for problems in timber-concrete bridges is nearly always poor detailing for durability and neglected maintenance. Mainly all aspects of timber protection and maintenance should therefore be considered even during the planning phase of bridge. Paper deals with evaluation of timber properties, system of opto-fiber sensors for monitoring and diagnostics of mechanical stress of timber-concrete bridges, and degradation of timber due to UV radiation, atmospheric conditions and biological agents after application of photocatalytic materials on surface.

As a proxy of community-engaged teaching and learning pedagogy in higher education institutions (HEIs), service-learning (SL) has just recently become a phenomenon of research and policy interest in the European Higher Education Area (EHEA). This study explores the first-ever service-learning experience of 246 students coming from three universities at Central and Eastern Europe (Slovakia, Romania and Croatia). The quantitative part of the survey was used to compare the differences between countries, whereas the qualitative part of the survey was a summative assessment, i.e., reflections on the satisfaction with SL experiences were collected through the open-ended questions of the survey, by treating the three countries as one dataset. Research findings speak in favour of students’ satisfaction with their first-ever SL experience, regardless of the country of their residence. Students from all three countries highly value their SL experience as it provided them with possibilities to learn a lot about the academic field and community as well, by facilitating their personal and professional contribution to the community. The legacy of SL courses relates with paradigmatic shifts in various academic aspects, like students and professors changing roles, students being placed in the centre of the learning process, connectedness of the curricula with the real-life setting, better understanding and appreciation of subjects studied. As for the non-academic related aspects, students’ reflection disclose their acknowledgment of personal growth and changes related with cognitive, emotional and behavioural dimensions. Our findings reveal that the unique experience students had with the SL courses shaped their narratives into ones of satisfaction and change.

Diffuse pulmonary meningotheliomatosis (DPM) is reported as a diffuse parenchymal lung disease characterized by disseminating small asymptomatic nodules. These lesions are often detected incidentally as microscopic findings in lung specimens or autopsies examined by a pathologist. We report a case of a 60-year-old male asymptomatic patient presenting with multiple bilateral pulmonary nodules on high-resolution computed tomography and diagnosed by videothoracoscopic surgery. Differential diagnosis of patients presenting with diffuse indeterminate nodules is very important. Definitive diagnosis of DPM requires histopathology and most often videothoracoscopic lung biopsy.

Long non-coding RNAs (lncRNAs) are functional RNAs longer than 200 nucleotides. Due to modern genomic techniques, the involvement of lncRNAs in tumorigenesis has been revealed; however, information concerning lncRNA interplay in multiple myeloma (MM) and plasma cell leukemia (PCL) is virtually absent. Herein, we aimed to identify the lncRNAs involved in MM to PCL progression. We investigated representative datasets of MM and PCL patients using next-generation sequencing. In total, 13 deregulated lncRNAs (p

Cadmium is a serious environmental pollutant and its uptake by plant represents a serious health risk. Uptake, accumulation as well as sensitivity of soybean plants to metals have been shown to vary with genotype, while the dynamics of this uptake has rarely been studied. Here we studied the uptake and accumulation of Cd2+ ions in different parts of soybean plants of four cultivars Moravians, Gallec, Kent and Cardiff. The plants at early developmental stage were immersed in Hoagland nutrient solution in the presence or absence of 50 mg.L−1 and the isotope of 109Cd2+ to monitor its accumulation continuously at 24 h intervals for 10 days. Our results showed that the uptake rate varied among the cultivars, being the highest in roots of the cv. Moravians and the lowest in the cv. Gallec. We also observed a non-even distribution of radioactivity within the entire plants of individual cultivars. The most of Cd2+ isotope was translocated into primary leaves and leaves in the cvs. Kent and Moravians; on the contrary, relatively less in the cvs. Cardiff and Gallec. The results were fitted with genetic potential, growth as well as defense parameters such as proline accumulation. Combining uptake dynamics and biochemical data are indicative for different tolerance strategies of soybeans.

Study objectives: Establishing standardized and controlled system of work at a clinical pharmacy department and establishing effective recording of activities of a group of four clinical pharmacist when providing clinical pharmaceutical care (CPC) in a hospital. Methods: The duration of evaluated period is 5.5 years. The first part was defining the purpose, methods and activities of clinical pharmaceutical care, the next part was designing the software for recording patient’s data and CPC activities. To verify the functionality of our system the third part was conducted (from January 1, 2015 to June 30, 2015). Results: CPC activities were defined precisely. During the 6 months period, 3946 patients were reviewed (17% of patients admitted), in this group, 41% patients was labeled as risk (these patients had one or more risk factor). 1722 repeated reviews were performed, 884 drug therapy recommendations were recorded. The calculated average time necessary for one CPC activity is 28 min. Conclusion: During the 5 year period, standardized system of work in clinical pharmacy department was established. This system is based on clearly defined activities and it enables external control. Our results supply data for negotiations with health insurance companies.

Defense components such as chitinases (EC 3.2.1.14) are crucial for plants to cope diseases. Despite of that the pattern and activities of these enzymes in agronomically important Triticale is unexplored. This work is aimed to study chitinase activities in the leaves of plants of early developmental stages in two diploids (Aegilops tauschii Coss., Triticum monococcum L.), four tetraploids (Ae. cylindrical Host, Ae. triuncialis L., T. araraticum Jakubyz, T. dicoccum Schrank) and two hexaploids (T. aestivum L., T. spelta L.). The leaves were subjected to quantitative and qualitative activity assays using synthetic 4-methylumbelliferyl-β-D-N,N´,N´´-triacetylchitotrioside and glycolchitin as substrates, respectively. Our results showed that the activities of chitinases with specificity towards short oligomers were variable and genotype dependent. The enzyme activities in the tetra- and hexaploid genotypes were significantly higher than in diplod counterparts. In the gel detection assays were revealed up to four fractions (~20, 30, 42 and 95 kDa) of proteins with the chitinase activity towards long chain polymers. The isoform of ~30 kDa was identified in all analyzed genotypes. Among the seven acidic and three basic chitinase fractions identified, three acidic (ChiA, ChiB, ChiC) and two (ChiH, ChiI) fractions were present in all genotypes. None of the isoforms can be assigned as specific with respect to ploidy.