1. Comparison of the diagnostic performance of bacterial culture of nasopharyngeal swab and bronchoalveolar lavage fluid samples obtained from calves with bovine respiratory disease
- Author
-
Keith D. DeDonder, Robert L. Larson, Dayna M. Harhay, Gennie Schuller, Michael D. Apley, Sarah F. Capik, Michael L. Clawson, Ted Kalbfleisch, Greg P. Harhay, Brad J. White, Carol G. Chitko-McKown, and Brian V. Lubbers
- Subjects
0301 basic medicine ,Male ,Veterinary medicine ,Microbiological culture ,Pasteurella multocida ,040301 veterinary sciences ,animal diseases ,Population ,Respiratory Tract Diseases ,Prevalence ,Bovine respiratory disease ,Cattle Diseases ,0403 veterinary science ,03 medical and health sciences ,Predictive Value of Tests ,Nasopharynx ,medicine ,Animals ,education ,Mannheimia haemolytica ,education.field_of_study ,General Veterinary ,medicine.diagnostic_test ,biology ,business.industry ,04 agricultural and veterinary sciences ,General Medicine ,respiratory system ,medicine.disease ,biology.organism_classification ,respiratory tract diseases ,030104 developmental biology ,Bronchoalveolar lavage ,Predictive value of tests ,Immunology ,Cattle ,Sample collection ,Macrolides ,Pasteurellaceae ,business ,Bronchoalveolar Lavage Fluid ,Genome, Bacterial - Abstract
OBJECTIVE To compare predictive values, extent of agreement, and gamithromycin susceptibility between bacterial culture results of nasopharyngeal swab (NPS) and bronchoalveolar lavage fluid (BALF) samples obtained from calves with bovine respiratory disease (BRD). ANIMALS 28 beef calves with clinical BRD. PROCEDURES Pooled bilateral NPS samples and BALF samples were obtained for bacterial culture from calves immediately before and at various times during the 5 days after gamithromycin (6 mg/kg, SC, once) administration. For each culture-positive sample, up to 12 Mannheimia haemolytica, 6 Pasteurella multocida, and 6 Histophilus somni colonies underwent gamithromycin susceptibility testing. Whole-genome sequencing was performed on all M haemolytica isolates. For paired NPS and BALF samples collected 5 days after gamithromycin administration, the positive and negative predictive values for culture results of NPS samples relative to those of BALF samples and the extent of agreement between the sampling methods were determined. RESULTS Positive and negative predictive values of NPS samples were 67% and 100% for M haemolytica, 75% and 100% for P multocida, and 100% and 96% for H somni. Extent of agreement between results for NPS and BALF samples was substantial for M haemolytica (κ, 0.71) and H somni (κ, 0.78) and almost perfect for P multocida (κ, 0.81). Gamithromycin susceptibility varied within the same sample and between paired NPS and BALF samples. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated culture results of NPS and BALF samples from calves with BRD should be interpreted cautiously considering disease prevalence within the population, sample collection relative to antimicrobial administration, and limitations of diagnostic testing methods.
- Published
- 2017