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Comparison of the diagnostic performance of bacterial culture of nasopharyngeal swab and bronchoalveolar lavage fluid samples obtained from calves with bovine respiratory disease
- Source :
- American journal of veterinary research. 78(3)
- Publication Year :
- 2017
-
Abstract
- OBJECTIVE To compare predictive values, extent of agreement, and gamithromycin susceptibility between bacterial culture results of nasopharyngeal swab (NPS) and bronchoalveolar lavage fluid (BALF) samples obtained from calves with bovine respiratory disease (BRD). ANIMALS 28 beef calves with clinical BRD. PROCEDURES Pooled bilateral NPS samples and BALF samples were obtained for bacterial culture from calves immediately before and at various times during the 5 days after gamithromycin (6 mg/kg, SC, once) administration. For each culture-positive sample, up to 12 Mannheimia haemolytica, 6 Pasteurella multocida, and 6 Histophilus somni colonies underwent gamithromycin susceptibility testing. Whole-genome sequencing was performed on all M haemolytica isolates. For paired NPS and BALF samples collected 5 days after gamithromycin administration, the positive and negative predictive values for culture results of NPS samples relative to those of BALF samples and the extent of agreement between the sampling methods were determined. RESULTS Positive and negative predictive values of NPS samples were 67% and 100% for M haemolytica, 75% and 100% for P multocida, and 100% and 96% for H somni. Extent of agreement between results for NPS and BALF samples was substantial for M haemolytica (κ, 0.71) and H somni (κ, 0.78) and almost perfect for P multocida (κ, 0.81). Gamithromycin susceptibility varied within the same sample and between paired NPS and BALF samples. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated culture results of NPS and BALF samples from calves with BRD should be interpreted cautiously considering disease prevalence within the population, sample collection relative to antimicrobial administration, and limitations of diagnostic testing methods.
- Subjects :
- 0301 basic medicine
Male
Veterinary medicine
Microbiological culture
Pasteurella multocida
040301 veterinary sciences
animal diseases
Population
Respiratory Tract Diseases
Prevalence
Bovine respiratory disease
Cattle Diseases
0403 veterinary science
03 medical and health sciences
Predictive Value of Tests
Nasopharynx
medicine
Animals
education
Mannheimia haemolytica
education.field_of_study
General Veterinary
medicine.diagnostic_test
biology
business.industry
04 agricultural and veterinary sciences
General Medicine
respiratory system
medicine.disease
biology.organism_classification
respiratory tract diseases
030104 developmental biology
Bronchoalveolar lavage
Predictive value of tests
Immunology
Cattle
Sample collection
Macrolides
Pasteurellaceae
business
Bronchoalveolar Lavage Fluid
Genome, Bacterial
Subjects
Details
- ISSN :
- 19435681
- Volume :
- 78
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- American journal of veterinary research
- Accession number :
- edsair.doi.dedup.....ed3d9b60afe786c47ee53618c86871df